Lag0012061 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012061
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr1: 36978704 .. 36979117 (-)
RNA-Seq ExpressionLag0012061
SyntenyLag0012061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGGAGATTGGGTTTTAGCTTGGTCATCTCATATTTGCACAATCCCTATTTGTGCTGAAGCAGCGACGGTTCTGGAAGGATTACGTTTCGTGCGTCGTCAAAATGTAGAAAGACGGACGGTTATGTCTGATTCTCTTGCCTTGGTAAAGGCTATCAGGGGTGGTGGAATCCCGGATTCCTGTATAATTATTTCAATCATGTGGGATATCCGTAGTCTTATTGCTTCTTTTCATAGGGTTAATTTTGTGCATGTTAATCGTCAGTTTAATAGGGTGTCTCATGAAGTGGCCCAGTTTGGCCTACGATCGAGTCCACATGAGTGGACTTCAAATTTTCCTGATTGGCTTTCTCTGTTGGTTAGTCAAGAATGCCATCTCTTTGTATCCCCTAGGGGAGATTTTCTCGTTTAA

mRNA sequence

ATGGTTGGAGATTGGGTTTTAGCTTGGTCATCTCATATTTGCACAATCCCTATTTGTGCTGAAGCAGCGACGGTTCTGGAAGGATTACGTTTCGTGCGTCGTCAAAATGTAGAAAGACGGACGGTTATGTCTGATTCTCTTGCCTTGGTAAAGGCTATCAGGGGTGGTGGAATCCCGGATTCCTGTATAATTATTTCAATCATGTGGGATATCCGTAGTCTTATTGCTTCTTTTCATAGGGTTAATTTTGTGCATGTTAATCGTCAGTTTAATAGGGTGTCTCATGAAGTGGCCCAGTTTGGCCTACGATCGAGTCCACATGAGTGGACTTCAAATTTTCCTGATTGGCTTTCTCTGTTGGTTAGTCAAGAATGCCATCTCTTTGTATCCCCTAGGGGAGATTTTCTCGTTTAA

Coding sequence (CDS)

ATGGTTGGAGATTGGGTTTTAGCTTGGTCATCTCATATTTGCACAATCCCTATTTGTGCTGAAGCAGCGACGGTTCTGGAAGGATTACGTTTCGTGCGTCGTCAAAATGTAGAAAGACGGACGGTTATGTCTGATTCTCTTGCCTTGGTAAAGGCTATCAGGGGTGGTGGAATCCCGGATTCCTGTATAATTATTTCAATCATGTGGGATATCCGTAGTCTTATTGCTTCTTTTCATAGGGTTAATTTTGTGCATGTTAATCGTCAGTTTAATAGGGTGTCTCATGAAGTGGCCCAGTTTGGCCTACGATCGAGTCCACATGAGTGGACTTCAAATTTTCCTGATTGGCTTTCTCTGTTGGTTAGTCAAGAATGCCATCTCTTTGTATCCCCTAGGGGAGATTTTCTCGTTTAA

Protein sequence

MVGDWVLAWSSHICTIPICAEAATVLEGLRFVRRQNVERRTVMSDSLALVKAIRGGGIPDSCIIISIMWDIRSLIASFHRVNFVHVNRQFNRVSHEVAQFGLRSSPHEWTSNFPDWLSLLVSQECHLFVSPRGDFLV
Homology
BLAST of Lag0012061 vs. NCBI nr
Match: KAF7086882.1 (hypothetical protein CFC21_090137 [Triticum aestivum])

HSP 1 Score: 60.5 bits (145), Expect = 1.3e-05
Identity = 38/119 (31.93%), Postives = 57/119 (47.90%), Query Frame = 0

Query: 12  HICTIPICAEAATVLEGLRFVRRQNVERRTVMSDSLALVKAIRGGGIPDSCIIISIMWDI 71
           ++C     AEA  V  GL         +  V SDS+ +V A+  G    S +  SI+ D 
Sbjct: 2   YLCFDSFTAEAIAVRFGLNLANTAGCSKNEVNSDSMEVVNALSQG--YSSSVASSIIDDC 61

Query: 72  RSLIASFHRVNFVHVNRQFNRVSHEVAQFGLRSSPHEWTSNFPDWLSLLVSQECHLFVS 131
             +   F  V + H NR+ NRV+HE+A+    SSP  W  N PD +  L+  +  L ++
Sbjct: 62  YFMSLGFSHVIYDHCNRERNRVAHELARLARFSSPSVWMDNAPDEVIPLLINDATLLMN 118

BLAST of Lag0012061 vs. NCBI nr
Match: OVA14796.1 (hypothetical protein BVC80_1143g10 [Macleaya cordata])

HSP 1 Score: 59.7 bits (143), Expect = 2.3e-05
Identity = 31/89 (34.83%), Postives = 49/89 (55.06%), Query Frame = 0

Query: 17  PICAEAATVLEGLRFVRRQNVERRTVMSDSLALVKAIRGGGIPDSCIIISIMWDIRSLIA 76
           PI AEA   L G++   + N     +  D + ++  I    +     I +I+ DIRSL++
Sbjct: 79  PIEAEALAFLLGIKLALKMNFFDCVIEGDVINIIHFINDNSLNIPWKIRAIILDIRSLVS 138

Query: 77  SFHRVNFVHVNRQFNRVSHEVAQFGLRSS 106
           SFH + FVH+ R  N+ +H +A+F L SS
Sbjct: 139 SFHSIGFVHITRACNKAAHNLAKFALNSS 167

BLAST of Lag0012061 vs. NCBI nr
Match: KAF7100170.1 (hypothetical protein CFC21_101709 [Triticum aestivum])

HSP 1 Score: 58.5 bits (140), Expect = 5.1e-05
Identity = 39/118 (33.05%), Postives = 56/118 (47.46%), Query Frame = 0

Query: 13  ICTIPICAEAATVLEGLRFVRRQNVERRTVMSDSLALVKAIRGGGIPDSCIIISIMWDIR 72
           IC     AEA  V  GL         +  V SDS+ +V A+  G    S +  SI+ D  
Sbjct: 3   ICFDSFTAEAIAVRFGLNLANTVGCSKIEVNSDSVEVVNALSQG--YSSSVASSIIDDCY 62

Query: 73  SLIASFHRVNFVHVNRQFNRVSHEVAQFGLRSSPHEWTSNFPDWLSLLVSQECHLFVS 131
            +   F  V + H NR+ NRV+HE+A+    SSP  W  N PD +  L+  +  L ++
Sbjct: 63  FMSLGFSHVIYDHCNRERNRVAHELAKLARFSSPRVWMDNAPDEVIPLLVNDATLLMN 118

BLAST of Lag0012061 vs. NCBI nr
Match: XP_027109537.1 (uncharacterized protein LOC113729441 [Coffea arabica])

HSP 1 Score: 58.5 bits (140), Expect = 5.1e-05
Identity = 36/118 (30.51%), Postives = 59/118 (50.00%), Query Frame = 0

Query: 3   GDWVLAWS--SHICTIPICAEAATVLEGLRFVRRQNVERRTVMSDSLALVKAIRGGGIPD 62
           G  V  W+  S  C++P+  EA  +   +     +  ER  + SD   ++  I+G    D
Sbjct: 134 GKLVATWACPSFTCSVPVLEEALAIRTAMVKAALEGWERIIIESDCKVVIDRIQGDS--D 193

Query: 63  SCIIISIMWDIRSLIASFHRVNFVHVNRQFNRVSHEVAQFGLR-SSPHEWTSNFPDWL 118
             +I +++ DI+ L  +F    F  V+R+FN VSH  A++ L   S  +W + FP WL
Sbjct: 194 DVLISTVLQDIKLLKQNFKECCFSFVHREFNSVSHTFARYALNLGSIVDWKACFPVWL 249

BLAST of Lag0012061 vs. NCBI nr
Match: BBH00303.1 (Ribonuclease H-like superfamily protein [Prunus dulcis])

HSP 1 Score: 57.8 bits (138), Expect = 8.7e-05
Identity = 34/105 (32.38%), Postives = 52/105 (49.52%), Query Frame = 0

Query: 14  CTIPICAEAATVLEGLRFVRRQNVERRTVMSDSLALVKAIRGGGIPDSCIIISIMWDIRS 73
           CT  + AEA  V E L +      ++  V SDSL+L+K +    + D   I  I++D+  
Sbjct: 489 CTSALMAEAEAVREALTWCLELGFDKVAVESDSLSLIKMLNKEDVVD-LEIEGILFDVHC 548

Query: 74  LIASFHRVNFVHVNRQFNRVSHEVAQFGLR-SSPHEWTSNFPDWL 118
           L    H V F++  R+ N+ +H VA +  R    H W    P+WL
Sbjct: 549 LSHRAHHVEFLYAPRKCNQAAHNVAAYAFRIGGRHSWDIVCPEWL 592

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAF7086882.11.3e-0531.93hypothetical protein CFC21_090137 [Triticum aestivum][more]
OVA14796.12.3e-0534.83hypothetical protein BVC80_1143g10 [Macleaya cordata][more]
KAF7100170.15.1e-0533.05hypothetical protein CFC21_101709 [Triticum aestivum][more]
XP_027109537.15.1e-0530.51uncharacterized protein LOC113729441 [Coffea arabica][more]
BBH00303.18.7e-0532.38Ribonuclease H-like superfamily protein [Prunus dulcis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 10..106
e-value: 2.9E-10
score: 42.2
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 15..100
e-value: 4.3E-14
score: 52.4
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 17..121
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 20..98
e-value: 1.22607E-10
score: 53.0868
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 19..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012061.1Lag0012061.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity