Lag0032917 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032917
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Autophagy-related protein
Location	chr11: 38864832 .. 38866647 (-)
RNA-Seq Expression	Lag0032917
Synteny	Lag0032917

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAAGAGCTACTTCAAGCAAGAGCATGATCTTGGTAGTTTTCAACTCCACGTTTCTAATATGCTTTTCTTTTTCCCTAGAGATTTTATCAAGTCCATTTTGTAAGACTAGAACTGATAAGATTTCCTTGTTTGTTTTACCAGAAAAAAGAAAAGCAGAGGCTGCAAGGATTAGGGAGAAATACCCTGATAGAATTCCAGTAAGAAATGATTTTATTTTGGAACCTTGATTTGATAGGCTATTGATTATCATCACCTTGATGTCAAGATATTGGTTTAACAAAGCTTGGATGTTTTTGTATTAAACTCCTCACTCTTAAGTAACAAGTCAAATTTGTTCATGTCAGCATCCTTTTCTGTCTGTCATTTGGCAAGGAAGCAATCAGTTCTTGATAATGTAGTCTTTGATTTCTTACACGAAACCTGGATAGTGTGTTTTAAGAATGAAATGTCTCAAAATGAAGGCAGTTCATCTGTTGTATTTTTGTTTGTGCACATGCTTCAACATTCTGTTGGTCACCACTTGTTTATAATTTTGCTTTCATGGTTTCAAGGTTATCGTGGAAAAGGCGGAGAGAAGTGACGTCCCTAGCATTGATAAGAAGAAGTAAGAATCACCCACAAGAGTTATACATTTGTTACATTTCTTCCTTTCGTAGTTCTCTGTTGGATTTACGATTCTTTTCTATTATATTAAAGGTGGAGTTGATTTTGTATAAAAATTTGGGGAAAGTATTCTGTGTTTATTTCTTTTCGACAAAGAAAATAGTAGACCAACTTAAGTATCACTCAACTGGTTAAGACATTATATCCATGGCCAAAAGATCACATATTTGAATCTCCACCCCTACTTGTGGTTGAACTCAAAAAAAAAAAAAAAAAAAAAACTACTGCTAGCTGATCAACTAAAATCTTCTTGCTTCTGTGTTGGAGTTTATATCCAATGACTACATTTCCAGAGATTTTTTCTCTTTTTAATTGATGCTGATGAGCTGTATATACACACAAACATTCGTTGCTGCTGAATATGGTCTTCATTTAATTTTTCAAAATTATCTTTCTGATCTTCATGGTTCTGTTGTCAATTTGCTTTATGCATATGATTCATCTCAAAATCACTAGGAGAGAATGGGGCAATTGGCAGTTTCCAATGCCTTTCACTTCTTTCTCTTTTTCTAGCTAAGATTTTGATGCAACAACTGCAGGTACCTAGTTCCAGCGGACCTTACCGTGGGACAGTTTGTTTATGTCATCCGTAAAAGGATTAAATTGAGCCCAGAGAAAGCGATCTTCATATTCGTGGACGATGTCCTCCCTCCTACAGGTACACCATCTTTGTCTATAATATATATCTGGTACATGTCTAGGACCACTTTTGAAAATGAAAGTCCATCTCATAGACAAGTTCATTTAACAGATTTTGAAAACCATCAGTTAGTTTCCTTGTCTGTTTCTTGGAATAAGTCTGACATGTTCGACTTATGATTACGATCTATCAATAAATGCCACGCAGTTACATGTTAACGTTAGTTTTGACATAATTGGTACACATAGCACTCACATGTACCCATAATTTAACAAAAGGGTATGTTGAAAATTTCGACAACTATATCGGTGTTTAAAACCAAAACTGAAACACGAGTATGATATGCAAGAATGAAGAACTATAACTTGTTATTGTCCAACTTGGCTGAACAAATCACTTATTTTTTACTTCTTAAGGAGCAATCATGTCTGCAATATATGAAGAGAAGAAGGATGAAGATGGATTTCTGTATGTTACTTACAGTGGAGAGAACACATTTGGATGGTAG

mRNA sequence

ATGGCTAAGAGCTACTTCAAGCAAGAGCATGATCTTGAAAAAAGAAAAGCAGAGGCTGCAAGGATTAGGGAGAAATACCCTGATAGAATTCCAGTTATCGTGGAAAAGGCGGAGAGAAGTGACGTCCCTAGCATTGATAAGAAGAAGTACCTAGTTCCAGCGGACCTTACCGTGGGACAGTTTGTTTATGTCATCCGTAAAAGGATTAAATTGAGCCCAGAGAAAGCGATCTTCATATTCGTGGACGATGTCCTCCCTCCTACAGGAGCAATCATGTCTGCAATATATGAAGAGAAGAAGGATGAAGATGGATTTCTGTATGTTACTTACAGTGGAGAGAACACATTTGGATGGTAG

Coding sequence (CDS)

ATGGCTAAGAGCTACTTCAAGCAAGAGCATGATCTTGAAAAAAGAAAAGCAGAGGCTGCAAGGATTAGGGAGAAATACCCTGATAGAATTCCAGTTATCGTGGAAAAGGCGGAGAGAAGTGACGTCCCTAGCATTGATAAGAAGAAGTACCTAGTTCCAGCGGACCTTACCGTGGGACAGTTTGTTTATGTCATCCGTAAAAGGATTAAATTGAGCCCAGAGAAAGCGATCTTCATATTCGTGGACGATGTCCTCCCTCCTACAGGAGCAATCATGTCTGCAATATATGAAGAGAAGAAGGATGAAGATGGATTTCTGTATGTTACTTACAGTGGAGAGAACACATTTGGATGGTAG

Protein sequence

MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQFVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW

Homology

[Close]

BLAST of Lag0032917 vs. NCBI nr
Match: XP_004152407.1 (autophagy-related protein 8f [Cucumis sativus] >XP_004152408.1 autophagy-related protein 8f [Cucumis sativus] >XP_008437098.1 PREDICTED: autophagy-related protein 8f [Cucumis melo] >XP_031742328.1 autophagy-related protein 8f [Cucumis sativus] >XP_038876330.1 autophagy-related protein 8f isoform X1 [Benincasa hispida] >XP_038876331.1 autophagy-related protein 8f isoform X1 [Benincasa hispida] >XP_038876332.1 autophagy-related protein 8f isoform X1 [Benincasa hispida] >KAA0039709.1 autophagy-related protein 8f [Cucumis melo var. makuwa] >KGN50246.1 hypothetical protein Csa_000352 [Cucumis sativus])

HSP 1 Score: 232.6 bits (592), Expect = 1.7e-57
Identity = 116/118 (98.31%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSD+PSIDKKKYLVPADLTVGQ
Sbjct: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDIPSIDKKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW 119
           FVYVIRKRIKLSPEKAIFIFVD+VLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW
Sbjct: 61  FVYVIRKRIKLSPEKAIFIFVDNVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW 118

[Close]

BLAST of Lag0032917 vs. NCBI nr
Match: XP_022154834.1 (autophagy-related protein 8f [Momordica charantia] >XP_022154835.1 autophagy-related protein 8f [Momordica charantia])

HSP 1 Score: 228.8 bits (582), Expect = 2.5e-56
Identity = 115/118 (97.46%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSD+PSIDKKKYLVPADLTVGQ
Sbjct: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDIPSIDKKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW 119
           FVYVIRKRIKLSPEKAIFIFVD+VLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFG+
Sbjct: 61  FVYVIRKRIKLSPEKAIFIFVDNVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGF 118

[Close]

BLAST of Lag0032917 vs. NCBI nr
Match: XP_022969729.1 (autophagy-related protein 8f [Cucurbita maxima])

HSP 1 Score: 228.8 bits (582), Expect = 2.5e-56
Identity = 115/118 (97.46%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSD+PSIDKKKYLVPADLTVGQ
Sbjct: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDIPSIDKKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW 119
           FVYVIRKRIKLSPEKAIFIFVD+VLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFG+
Sbjct: 61  FVYVIRKRIKLSPEKAIFIFVDNVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGF 118

[Close]

BLAST of Lag0032917 vs. NCBI nr
Match: XP_022922412.1 (autophagy-related protein 8f [Cucurbita moschata] >XP_023549494.1 autophagy-related protein 8f [Cucurbita pepo subsp. pepo] >KAG7016894.1 Autophagy-related protein 8f [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 228.8 bits (582), Expect = 2.5e-56
Identity = 115/118 (97.46%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSD+PSIDKKKYLVPADLTVGQ
Sbjct: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDIPSIDKKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW 119
           FVYVIRKRIKLSPEKAIFIFVD+VLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFG+
Sbjct: 61  FVYVIRKRIKLSPEKAIFIFVDNVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGF 118

[Close]

BLAST of Lag0032917 vs. NCBI nr
Match: KAG6579417.1 (Autophagy-related protein 8f, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 227.6 bits (579), Expect = 5.5e-56
Identity = 114/118 (96.61%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSD+PSIDKKKYLVPADLTVGQ
Sbjct: 35  MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDIPSIDKKKYLVPADLTVGQ 94

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW 119
           FVYVIR+RIKLSPEKAIFIFVD+VLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFG+
Sbjct: 95  FVYVIRRRIKLSPEKAIFIFVDNVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGF 152

[Close]

BLAST of Lag0032917 vs. ExPASy Swiss-Prot
Match: Q8VYK7 (Autophagy-related protein 8f OS=Arabidopsis thaliana OX=3702 GN=ATG8F PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.4e-54
Identity = 105/118 (88.98%), Postives = 115/118 (97.46%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKS FKQEHDLEKR+AEAARIREKYPDRIPVIVEKAE+SD+P+IDKKKYLVPADLTVGQ
Sbjct: 1   MAKSSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFGW 119
           FVYVIRKRIKLS EKAIFIFVD+VLPP GA+MS++YEEKKD+DGFLYVTYSGENTFG+
Sbjct: 61  FVYVIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFGF 118

[Close]

BLAST of Lag0032917 vs. ExPASy Swiss-Prot
Match: M1C146 (Autophagy-related protein 8C-like OS=Solanum tuberosum OX=4113 GN=ATG8CL PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 4.2e-51
Identity = 101/117 (86.32%), Postives = 109/117 (93.16%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKS FK EH LE+R+AEAARIREKYPDRIPVIVEKAERSD+P IDKKKYLVPADLTVGQ
Sbjct: 1   MAKSSFKLEHPLERRQAEAARIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFG 118
           FVYV+RKRIKLS EKAIFIFV ++LPPT A+MSAIYEE KDEDGFLY+TYSGENTFG
Sbjct: 61  FVYVVRKRIKLSAEKAIFIFVKNILPPTAAMMSAIYEEHKDEDGFLYMTYSGENTFG 117

[Close]

BLAST of Lag0032917 vs. ExPASy Swiss-Prot
Match: Q9SL04 (Autophagy-related protein 8d OS=Arabidopsis thaliana OX=3702 GN=ATG8D PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.2e-50
Identity = 100/117 (85.47%), Postives = 109/117 (93.16%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MA S FK EH LEKR+AEAARIREKYPDRIPVIVE+AE+SDVP ID+KKYLVPADLTVGQ
Sbjct: 1   MAISSFKHEHPLEKRQAEAARIREKYPDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFG 118
           FVYV+RKRIKLSPEKAIFIFV ++LPPT AIMSAIYEE KDEDGFLY++YSGENTFG
Sbjct: 61  FVYVVRKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 117

[Close]

BLAST of Lag0032917 vs. ExPASy Swiss-Prot
Match: A2XXR7 (Autophagy-related protein 8B OS=Oryza sativa subsp. indica OX=39946 GN=ATG8B PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 6.1e-50
Identity = 99/117 (84.62%), Postives = 107/117 (91.45%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKS FK +H LE+R+AEA RIREKY DRIPVIVEKAERSD+P IDKKKYLVPADLTVGQ
Sbjct: 1   MAKSSFKLDHTLERRQAEANRIREKYSDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFG 118
           FVYV+RKRIKLSPEKAIFIFV + LPPT A+MSAIYEE KDEDGFLY+TYSGENTFG
Sbjct: 61  FVYVVRKRIKLSPEKAIFIFVKNTLPPTAALMSAIYEENKDEDGFLYMTYSGENTFG 117

[Close]

BLAST of Lag0032917 vs. ExPASy Swiss-Prot
Match: Q7XPR1 (Autophagy-related protein 8B OS=Oryza sativa subsp. japonica OX=39947 GN=ATG8B PE=3 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 6.1e-50
Identity = 99/117 (84.62%), Postives = 107/117 (91.45%), Query Frame = 0


Query: 1   MAKSYFKQEHDLEKRKAEAARIREKYPDRIPVIVEKAERSDVPSIDKKKYLVPADLTVGQ 60
           MAKS FK +H LE+R+AEA RIREKY DRIPVIVEKAERSD+P IDKKKYLVPADLTVGQ
Sbjct: 1   MAKSSFKLDHTLERRQAEANRIREKYSDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQ 60

Query: 61  FVYVIRKRIKLSPEKAIFIFVDDVLPPTGAIMSAIYEEKKDEDGFLYVTYSGENTFG 118
           FVYV+RKRIKLSPEKAIFIFV + LPPT A+MSAIYEE KDEDGFLY+TYSGENTFG
Sbjct: 61  FVYVVRKRIKLSPEKAIFIFVKNTLPPTAALMSAIYEENKDEDGFLYMTYSGENTFG 117

The following BLAST results are available for this feature:

BLAST of Lag0032917 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152407.1	1.7e-57	98.31	autophagy-related protein 8f [Cucumis sativus] >XP_004152408.1 autophagy-related...	[more]
XP_022154834.1	2.5e-56	97.46	autophagy-related protein 8f [Momordica charantia] >XP_022154835.1 autophagy-rel...	[more]
XP_022969729.1	2.5e-56	97.46	autophagy-related protein 8f [Cucurbita maxima]	[more]
XP_022922412.1	2.5e-56	97.46	autophagy-related protein 8f [Cucurbita moschata] >XP_023549494.1 autophagy-rela...	[more]
KAG6579417.1	5.5e-56	96.61	Autophagy-related protein 8f, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0032917 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYK7	1.4e-54	88.98	Autophagy-related protein 8f OS=Arabidopsis thaliana OX=3702 GN=ATG8F PE=1 SV=1	[more]
M1C146	4.2e-51	86.32	Autophagy-related protein 8C-like OS=Solanum tuberosum OX=4113 GN=ATG8CL PE=1 SV...	[more]
Q9SL04	1.2e-50	85.47	Autophagy-related protein 8d OS=Arabidopsis thaliana OX=3702 GN=ATG8D PE=1 SV=1	[more]
A2XXR7	6.1e-50	84.62	Autophagy-related protein 8B OS=Oryza sativa subsp. indica OX=39946 GN=ATG8B PE=...	[more]
Q7XPR1	6.1e-50	84.62	Autophagy-related protein 8B OS=Oryza sativa subsp. japonica OX=39947 GN=ATG8B P...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004241	Autophagy protein Atg8 ubiquitin-like	PFAM	PF02991	Atg8	coord: 14..117 e-value: 5.4E-51 score: 171.1
IPR004241	Autophagy protein Atg8 ubiquitin-like	PANTHER	PTHR10969	MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED	coord: 1..118
None	No IPR available	GENE3D	3.10.20.90		coord: 1..117 e-value: 7.2E-59 score: 199.2
None	No IPR available	PANTHER	PTHR10969:SF77	AUTOPHAGY-RELATED PROTEIN	coord: 1..118
None	No IPR available	CDD	cd16128	Ubl_ATG8	coord: 12..114 e-value: 9.41931E-71 score: 204.617
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 3..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032917.1	Lag0032917.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006914	autophagy
biological_process	GO:0006995	cellular response to nitrogen starvation
cellular_component	GO:0016020	membrane
cellular_component	GO:0005874	microtubule