Lag0030711 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0030711
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionheavy metal-associated isoprenylated plant protein 39-like
Locationchr11: 644037 .. 645172 (+)
RNA-Seq ExpressionLag0030711
SyntenyLag0030711
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGTACGACATGTCGACTTCTTTGTCTTTCTGGTTTTTTCTTTGGGGGGTTCTGTTTTTCTGATCTTTGATCTTTTTTTTTTTTTTTTTTTTTGCAGAAGGTGATTGTGAAATTGGATGTTCAAGATGATAAATCTAAGCAAAAAGCTATGAGCACTGTGTCTAGTCTATCAGGTACTCAATTGAATTTCTGAATTTGTCCAAATCAGAATTTATTTTCATTCTTGATGGATCATTGAAATAACCCATCATTTATTAGAGAAACAAAATCAACCCCATCTCTTAAAACCTGTCACTTGAGTCTCTCTCTGTTTTTCTCCAAACCAAATGAATTTGCTTTTTTCATTTTTCATGGAAAATTGAAAGAACCCATTATTTTACAAGCAAAACAAAAACAAACCCATGTCTTAAAATCAGTCATCTCTCTCTCTCTCTCTTTTTCTGTCTTGTTTGGCTTATGAGAAAACAGAAGAAAAGAAAACACAAAAACCAAAAAAGTTTCCTTTTTCCCTCCTTTTCATGGGAAAATCAAAAGGACCCATTATTTCACAAGAAAAACAAAAACAAACCCATGTCTTGATTCTTAAAATCAGCCATCTTTCTGTCTCTCTATCCTGTTTGGCTTCAGAGAAAACAGAAGAAAAGAAAACCAAAACCAAAAAAATTCCCCCTTTTCCCCTTTTCATGGAAAATCAAAAGGACCCATTACTTCACAAGAAAAACAAAAACAAACCCATGTCTAAAAATCATCCATCTTTGTCCTGTTTGGCTTAAAATTGATTGGGTAAATTCTTGCAGGAGTGAACTCAATCTCAATGGACATGAAAGAGAAGAAACTGACAGTAACAGGGGATGTAGATCCAGTGGTGATAGTGAGCAAACTGAGGAAAATTTGTCACACAGTGATAGTATCAGTTGGACCAGAGAAAGAAGAGAAGAAACCAGAGCCAAAGAAAGATGAGCCTAAGAAGGAAGATCCCAAAAAGGCAGCTGAGGAAAAGAAGAAAGAAGAACAAAGAATTGCTGAGTATATCAAGGCTATGGAAGCCTATCGTAAACTCTATCCACCAATGTATTATCCTCCTAGAACCATAAGCATTGAAGAAGATCCAAATGGTTGTGTTATTTGTTAA

mRNA sequence

ATGAAGAAGGTGATTGTGAAATTGGATGTTCAAGATGATAAATCTAAGCAAAAAGCTATGAGCACTGTGTCTAGTCTATCAGGAGTGAACTCAATCTCAATGGACATGAAAGAGAAGAAACTGACAGTAACAGGGGATGTAGATCCAGTGGTGATAGTGAGCAAACTGAGGAAAATTTGTCACACAGTGATAGTATCAGTTGGACCAGAGAAAGAAGAGAAGAAACCAGAGCCAAAGAAAGATGAGCCTAAGAAGGAAGATCCCAAAAAGGCAGCTGAGGAAAAGAAGAAAGAAGAACAAAGAATTGCTGAGTATATCAAGGCTATGGAAGCCTATCGTAAACTCTATCCACCAATGTATTATCCTCCTAGAACCATAAGCATTGAAGAAGATCCAAATGGTTGTGTTATTTGTTAA

Coding sequence (CDS)

ATGAAGAAGGTGATTGTGAAATTGGATGTTCAAGATGATAAATCTAAGCAAAAAGCTATGAGCACTGTGTCTAGTCTATCAGGAGTGAACTCAATCTCAATGGACATGAAAGAGAAGAAACTGACAGTAACAGGGGATGTAGATCCAGTGGTGATAGTGAGCAAACTGAGGAAAATTTGTCACACAGTGATAGTATCAGTTGGACCAGAGAAAGAAGAGAAGAAACCAGAGCCAAAGAAAGATGAGCCTAAGAAGGAAGATCCCAAAAAGGCAGCTGAGGAAAAGAAGAAAGAAGAACAAAGAATTGCTGAGTATATCAAGGCTATGGAAGCCTATCGTAAACTCTATCCACCAATGTATTATCCTCCTAGAACCATAAGCATTGAAGAAGATCCAAATGGTTGTGTTATTTGTTAA

Protein sequence

MKKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKICHTVIVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQRIAEYIKAMEAYRKLYPPMYYPPRTISIEEDPNGCVIC
Homology
BLAST of Lag0030711 vs. NCBI nr
Match: XP_008437534.1 (PREDICTED: FK506-binding protein 4-like [Cucumis melo] >ADN33773.1 metal ion binding protein [Cucumis melo subsp. melo] >KAA0042592.1 FK506-binding protein 4-like [Cucumis melo var. makuwa] >TYK05995.1 FK506-binding protein 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 223.0 bits (567), Expect = 1.6e-54
Identity = 124/141 (87.94%), Postives = 131/141 (92.91%), Query Frame = 0

Query: 1   MKKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60
           MKKVIVKLDV D+KSKQKAMS VSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC
Sbjct: 1   MKKVIVKLDVSDEKSKQKAMSVVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60

Query: 61  HTVIVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQRIAEYIKAMEAYRKLY---P 120
           HT IVSVGPEKEEKKPEPKKDEPKKEDPKK AEEKKKEEQR+AE+IKA +AY  LY   P
Sbjct: 61  HTTIVSVGPEKEEKKPEPKKDEPKKEDPKKVAEEKKKEEQRLAEFIKACQAY-NLYNNPP 120

Query: 121 PMYYPPRTISIEEDPNGCVIC 139
           P++YPPR+ISIEEDPNGCVIC
Sbjct: 121 PVFYPPRSISIEEDPNGCVIC 140

BLAST of Lag0030711 vs. NCBI nr
Match: XP_022922258.1 (heavy metal-associated isoprenylated plant protein 39-like [Cucurbita moschata] >XP_023549811.1 heavy metal-associated isoprenylated plant protein 39-like [Cucurbita pepo subsp. pepo] >KAG6579367.1 Heavy metal-associated isoprenylated plant protein 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 212.6 bits (540), Expect = 2.1e-51
Identity = 116/139 (83.45%), Postives = 129/139 (92.81%), Query Frame = 0

Query: 1   MKKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60
           MKKVI+KLDV D+KSKQKAMS+VSSL+GVNSISMDMKEKKLTVTGDVDPV+IVSKLRKIC
Sbjct: 1   MKKVILKLDVSDEKSKQKAMSSVSSLAGVNSISMDMKEKKLTVTGDVDPVMIVSKLRKIC 60

Query: 61  HTVIVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQRIAEYIKAMEAYRK--LYPP 120
           HTVIVSVGPEKEEKKPEPKK+EPKKEDPKKAAEEKKKEE+++AE +KA  A      YPP
Sbjct: 61  HTVIVSVGPEKEEKKPEPKKEEPKKEDPKKAAEEKKKEEEKLAEMLKAWHARNNPCFYPP 120

Query: 121 MYYPPRTISIEEDPNGCVI 138
           ++YPPR+ISIEEDPNGCVI
Sbjct: 121 VFYPPRSISIEEDPNGCVI 139

BLAST of Lag0030711 vs. NCBI nr
Match: XP_004145901.1 (heavy metal-associated isoprenylated plant protein 39 [Cucumis sativus] >KGN49919.1 hypothetical protein Csa_000191 [Cucumis sativus])

HSP 1 Score: 211.5 bits (537), Expect = 4.7e-51
Identity = 122/144 (84.72%), Postives = 128/144 (88.89%), Query Frame = 0

Query: 1   MKKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60
           MKKVIVKLDV D+KSKQKAMS VSSLSGVNSISMDMKEKKLTVTGDVDPVVIV KLRKIC
Sbjct: 1   MKKVIVKLDVSDEKSKQKAMSVVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVGKLRKIC 60

Query: 61  HTVIVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQRIAEYIKA---MEAYRKLY- 120
           HT IVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQ++AE IKA    +AY  LY 
Sbjct: 61  HTTIVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQKLAELIKACQQYQAYNYLYC 120

Query: 121 -PPMYY-PPRTISIEEDPNGCVIC 139
            PP++Y PPR ISIEEDPN CVIC
Sbjct: 121 NPPVFYPPPRCISIEEDPNACVIC 144

BLAST of Lag0030711 vs. NCBI nr
Match: XP_022973069.1 (heavy metal-associated isoprenylated plant protein 39-like [Cucurbita maxima])

HSP 1 Score: 210.7 bits (535), Expect = 8.1e-51
Identity = 116/139 (83.45%), Postives = 128/139 (92.09%), Query Frame = 0

Query: 1   MKKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60
           MKKVI+KLDV D+KSKQKAMS+VSSL+GVNSISMDMKEKKLTVTGDVDPV+IVSKLRKIC
Sbjct: 1   MKKVILKLDVSDEKSKQKAMSSVSSLAGVNSISMDMKEKKLTVTGDVDPVMIVSKLRKIC 60

Query: 61  HTVIVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQRIAEYIKAMEAYRK--LYPP 120
           HTVIVSVGPEKEEKKPEPKK+EPKKEDPKKAAEEKKKEE+++AE +KA  A      YPP
Sbjct: 61  HTVIVSVGPEKEEKKPEPKKEEPKKEDPKKAAEEKKKEEEKLAEMLKAWHARNNPCFYPP 120

Query: 121 MYYPPRTISIEEDPNGCVI 138
           + YPPR+ISIEEDPNGCVI
Sbjct: 121 ICYPPRSISIEEDPNGCVI 139

BLAST of Lag0030711 vs. NCBI nr
Match: XP_038906483.1 (heavy metal-associated isoprenylated plant protein 39-like [Benincasa hispida])

HSP 1 Score: 210.3 bits (534), Expect = 1.1e-50
Identity = 116/141 (82.27%), Postives = 130/141 (92.20%), Query Frame = 0

Query: 1   MKKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60
           M+KV++KLDV ++K+KQKAMS VSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC
Sbjct: 1   MRKVVMKLDVSEEKTKQKAMSVVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60

Query: 61  HTVIVSVGPEKEEKKPEP-KKDEPKKEDPKKAAEEKKKEEQRIAEYIKAMEAYRKLY--P 120
           HTVIVSVGPEKEEKKPEP KK+ PKK+DPKKAAEEKKKEEQ++AE+IKA EAY  L+  P
Sbjct: 61  HTVIVSVGPEKEEKKPEPAKKEPPKKDDPKKAAEEKKKEEQKLAEFIKACEAYNNLHYNP 120

Query: 121 PMYYPPRTISIEEDPNGCVIC 139
            ++YPPR ISIEEDPNGCVIC
Sbjct: 121 SIFYPPRCISIEEDPNGCVIC 141

BLAST of Lag0030711 vs. ExPASy Swiss-Prot
Match: O03982 (Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX=3702 GN=HIPP39 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 4.8e-22
Identity = 82/180 (45.56%), Postives = 102/180 (56.67%), Query Frame = 0

Query: 1   MKKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60
           MKK+++KLD+ DD++KQKA+ TVS+L G++SI+MDMKEKKLTV G VDPV +VSKLRK  
Sbjct: 1   MKKIVLKLDLHDDRAKQKALKTVSTLPGIDSIAMDMKEKKLTVIGTVDPVNVVSKLRKYW 60

Query: 61  -HTVIVSVGPEKE---EKKPEPKKD----------EPKKEDPKKAAEEKKKEEQR----- 120
             T IV VGP KE   EKK EPKK+          E  KE+ KK  E  KKEE++     
Sbjct: 61  PMTDIVLVGPAKEPEKEKKEEPKKEGGGEPPKKEGEAPKEEGKKEGEAPKKEEEKKEGGD 120

Query: 121 -----------------------IAEYIKAMEAYRKLYPPMYYPPRTISIEEDPNGCVIC 139
                                  + E +KA +AY       YY     SIEE+PN CVIC
Sbjct: 121 KKEGEKKDQPQAQPQPVVPPPDHVLELVKAYKAYNPHLTTYYYAQ---SIEENPNACVIC 177

BLAST of Lag0030711 vs. ExPASy Swiss-Prot
Match: Q9LTE3 (Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX=3702 GN=HIPP12 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-16
Identity = 62/136 (45.59%), Postives = 81/136 (59.56%), Query Frame = 0

Query: 1   MKKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKIC 60
           M+ V++KLDV  +K+KQKAMSTV  LSGVNS+  ++K+ KLTVTG++D  +IV KL+KIC
Sbjct: 1   MQVVVLKLDVHCEKTKQKAMSTVCCLSGVNSV--EVKDGKLTVTGEIDAYMIVKKLKKIC 60

Query: 61  HTVIVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQRIAEYIKAMEAYRKLYPPMY 120
           HT  +SVGP KE +K +P  D+PKK +         K    I  Y+           P Y
Sbjct: 61  HTEFISVGPVKEPEKKKP--DDPKKPE--------TKPPDVIYHYVPPC--------PPY 116

Query: 121 YPPRTISIEEDPNGCV 137
           Y        EDPN CV
Sbjct: 121 YHNFNGCYNEDPNACV 116

BLAST of Lag0030711 vs. ExPASy Swiss-Prot
Match: Q9LTE2 (Heavy metal-associated isoprenylated plant protein 13 OS=Arabidopsis thaliana OX=3702 GN=HIPP13 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.5e-10
Identity = 55/138 (39.86%), Postives = 77/138 (55.80%), Query Frame = 0

Query: 3   KVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKICHT 62
           K +++L + +++ ++KA  TVS   GV SI+MD K  K+TV G+VD  VIV KLRK+C+T
Sbjct: 5   KAVLQLSIHEERIRKKAFVTVSRCPGVTSITMDDKTGKMTVVGEVDVPVIVMKLRKLCNT 64

Query: 63  VIVSVGPEKEEKKPEPKKDEPKKEDPKKAAEEKKKEEQRIAEYIKAMEAYR--KLYPPMY 122
            +VSV    E  KP  KK EP+K  P K A    K  + +A  ++    Y+    Y   Y
Sbjct: 65  ELVSV----EVVKPPEKKPEPEKPAPPKPAPAPAKPAEIVAWPVQMNNPYQYNPAYANSY 124

Query: 123 YPPRTIS-IEEDPNGCVI 138
           Y P   S    D + CVI
Sbjct: 125 YQPYGNSRFVTDESNCVI 138

BLAST of Lag0030711 vs. ExPASy Swiss-Prot
Match: Q9LTE1 (Heavy metal-associated isoprenylated plant protein 14 OS=Arabidopsis thaliana OX=3702 GN=HIPP14 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.1e-09
Identity = 38/84 (45.24%), Postives = 59/84 (70.24%), Query Frame = 0

Query: 2  KKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKICH 61
          K  +++L + ++++++KA+ TVS  SGV SI+MD K  K+T+ G+VD   +V KLRK+C+
Sbjct: 4  KNAVLQLSIHEERTRKKALVTVSRFSGVTSITMD-KSGKMTIVGEVDVPAVVMKLRKLCN 63

Query: 62 TVIVSVGPEKEEKKPEPKKDEPKK 86
          T IVSV    ++ KP  KK EP+K
Sbjct: 64 TEIVSV----DDVKPPVKKPEPEK 82

BLAST of Lag0030711 vs. ExPASy Swiss-Prot
Match: Q8GWS3 (Heavy metal-associated isoprenylated plant protein 2 OS=Arabidopsis thaliana OX=3702 GN=HIPP02 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.4e-08
Identity = 36/82 (43.90%), Postives = 55/82 (67.07%), Query Frame = 0

Query: 2  KKVIVKLDVQDDKSKQKAMSTVSSLSGVNSISMDMKEKKLTVTGDVDPVVIVSKLRKI-C 61
          KK+ +K+D+  +K K   M  V+ L GVN +S+D ++  LTV G +DPV +  +L+KI  
Sbjct: 4  KKIEIKVDIDCEKCKHAIMEAVTELEGVNIVSLDQEKSILTVVGTMDPVCVAEQLKKINK 63

Query: 62 HTVIVSVG-PEKEEKKPEPKKD 82
            V++SVG P+K + KP+PKKD
Sbjct: 64 KPVVISVGPPKKPDPKPDPKKD 85

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008437534.11.6e-5487.94PREDICTED: FK506-binding protein 4-like [Cucumis melo] >ADN33773.1 metal ion bin... [more]
XP_022922258.12.1e-5183.45heavy metal-associated isoprenylated plant protein 39-like [Cucurbita moschata] ... [more]
XP_004145901.14.7e-5184.72heavy metal-associated isoprenylated plant protein 39 [Cucumis sativus] >KGN4991... [more]
XP_022973069.18.1e-5183.45heavy metal-associated isoprenylated plant protein 39-like [Cucurbita maxima][more]
XP_038906483.11.1e-5082.27heavy metal-associated isoprenylated plant protein 39-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O039824.8e-2245.56Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX... [more]
Q9LTE31.4e-1645.59Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX... [more]
Q9LTE22.5e-1039.86Heavy metal-associated isoprenylated plant protein 13 OS=Arabidopsis thaliana OX... [more]
Q9LTE16.1e-0945.24Heavy metal-associated isoprenylated plant protein 14 OS=Arabidopsis thaliana OX... [more]
Q8GWS31.4e-0843.90Heavy metal-associated isoprenylated plant protein 2 OS=Arabidopsis thaliana OX=... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 95..115
NoneNo IPR availableGENE3D3.30.70.100coord: 1..73
e-value: 2.7E-21
score: 77.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..99
NoneNo IPR availablePANTHERPTHR45811:SF60HEAVY METAL-ASSOCIATED DOMAIN PROTEINcoord: 1..138
NoneNo IPR availablePANTHERPTHR45811COPPER TRANSPORT PROTEIN FAMILY-RELATEDcoord: 1..138
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 9..58
e-value: 7.4E-9
score: 35.9
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 2..64

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0030711.1Lag0030711.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding