Sed0019920 (gene) Chayote v1

Overview


Name	Sed0019920
Type	gene
Organism	Sechium edule (Chayote v1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	LG01: 5118547 .. 5120453 (+)
RNA-Seq Expression	Sed0019920
Synteny	Sed0019920

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAATTATATCCTGAGGGTCACCCTTATTTTTTTCAGAACTTCAAAGCCTAAAAAAAATGTCGAATGATTCAGAAAACCTGTTGGAGGAGAAAGATAAGCAAACATTACAACATATGGAAGATATCACGAGCAACGCCGACGAAATCCAACGACAAATTCTAGTCGAAATTTTGTCAACAAATGGCCATGTTGAGTACTTGCAGCAACATGGCCTTAACGGATCTACTGATGCTTCAACTTTCAAGAACCTTATACCTGTCGTATCCTATGAACAACTGAAACCTTATATAGATCGTATTGCGTACGGTGATGATTCTCCAATCCTATGCGCACAACCCATCACACAGTTCTTTATCAGGTGAACCTTTTTTCTAAACCATAATCTTGTAAACTTATAATTAGTTTAATTTACTTGCCATCATGGATTTGTGTAACTTTTGTAGATCCTTATTTTTTTAGATAGCCTTAATGTGTGCAATATTAAAATTAAAAATGTATTTTTCTTGTAGCTCTGGAACATCGGGGGGAGAACAAAAGCTAATTCCAATGTTTGAACAAGAAGTTGAGAGAATTTTCTCTCCTATAATGAAGTATGAGATCCCAAAGATGAAACAATTGTTTCCAAATTCGCACAAAGGCAAAGAAATGTTCTTTCTTTTCAACAGGCCTCAGGCAAGAACTAAATCAGGAATTATTGCTCAATCTGTATCTACAAGTTTTTGCAAGAAACCACACTTATTTAACAATATACTGACTAGAGGTTGCACAAGCCCTAACGGCGTCCTTTTATGCACAGACACATACCAAAGCACATATTCTCAGTTACTTTGTGGGCTCTACCAAAACGAGTCAGTTTTTATATTGGGTTCTCTTTTTATCTCCACTCTAGCTTATGTTCTTAAGTTCCTCGAAAATCATTGGATTCTTTTGGCTAATGATATACGAACAGGAATCATAAACTCCAAAATTACAGATTCATTAGTGAGAGAATCAGTTATGAATATTCTTGAACCTAATCCCCACCTTGCAGATTTTATTGAAACAGAATGTAGCAAAGGAACATGGGAAGGAATCATAACAAGATTGTGGCCAAATGCCAAATACATTAAAGCTATTGCGACAGGAAGCATGTCACAATACATTCTCCAGCTAAATTACTACAGCAACAACCTCCCTCTTTGCTTCCTTGGCTTAAACCTAGATCCATTATGCAAACCAGAGGAAATCAGCTACACTTTAATCCCCACTATGGCATATTTTGAGTTTTTACCAATTGAAAGGGAAAAACCTAATGGGGAATCAAGACAAGATTTAGTGGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTCGTCGTCACCACTTACGCGGGGCTCTACCGTTATTGCATGGGTGACATTCTTCGTGTAACAGGGTTTAAAAATAAAGCCCCGAACTTTAGTTTTGTATGTAGGAGGAACATAATTCTTAGCCTGGATTTAGAGAAAACAGAGGAAGGTGAGCTTCACAATGCTATACAAGAAGGTGCTCGTCTTTTAGGTCCATTTGGAGCTGTTGTTGCTGACTACACAAGCTATGCAGATAACAGAACAATTCCTGGACACTATGTATTGTATTGGGAAATTCGTATTAAGGAAGAAAGTTCCATTATCCCATCTTCGATCTATGAGGATTGTTGCCCCAAATGGGATCCCAGTAGTCGACACCATCTTTTTTCTGCTTAGTTGATGAGAGAAAATTAATTATCTAGCAAGTGTTTTTAAAGTTGTTGTGATGCATTTGCAGTATTGTTTGTTGTGGTTACTTTTGTGTAATAGATGACTTTAACCTCTCATTTTCGTTTATTTGGTTTTTTTTACTCTTGATATGGTCAAACAAAATCTTATCATAAAGTGGTTTACA

mRNA sequence

CAAATTATATCCTGAGGGTCACCCTTATTTTTTTCAGAACTTCAAAGCCTAAAAAAAATGTCGAATGATTCAGAAAACCTGTTGGAGGAGAAAGATAAGCAAACATTACAACATATGGAAGATATCACGAGCAACGCCGACGAAATCCAACGACAAATTCTAGTCGAAATTTTGTCAACAAATGGCCATGTTGAGTACTTGCAGCAACATGGCCTTAACGGATCTACTGATGCTTCAACTTTCAAGAACCTTATACCTGTCGTATCCTATGAACAACTGAAACCTTATATAGATCGTATTGCGTACGGTGATGATTCTCCAATCCTATGCGCACAACCCATCACACAGTTCTTTATCAGCTCTGGAACATCGGGGGGAGAACAAAAGCTAATTCCAATGTTTGAACAAGAAGTTGAGAGAATTTTCTCTCCTATAATGAAGTATGAGATCCCAAAGATGAAACAATTGTTTCCAAATTCGCACAAAGGCAAAGAAATGTTCTTTCTTTTCAACAGGCCTCAGGCAAGAACTAAATCAGGAATTATTGCTCAATCTGTATCTACAAGTTTTTGCAAGAAACCACACTTATTTAACAATATACTGACTAGAGGTTGCACAAGCCCTAACGGCGTCCTTTTATGCACAGACACATACCAAAGCACATATTCTCAGTTACTTTGTGGGCTCTACCAAAACGAGTCAGTTTTTATATTGGGTTCTCTTTTTATCTCCACTCTAGCTTATGTTCTTAAGTTCCTCGAAAATCATTGGATTCTTTTGGCTAATGATATACGAACAGGAATCATAAACTCCAAAATTACAGATTCATTAGTGAGAGAATCAGTTATGAATATTCTTGAACCTAATCCCCACCTTGCAGATTTTATTGAAACAGAATGTAGCAAAGGAACATGGGAAGGAATCATAACAAGATTGTGGCCAAATGCCAAATACATTAAAGCTATTGCGACAGGAAGCATGTCACAATACATTCTCCAGCTAAATTACTACAGCAACAACCTCCCTCTTTGCTTCCTTGGCTTAAACCTAGATCCATTATGCAAACCAGAGGAAATCAGCTACACTTTAATCCCCACTATGGCATATTTTGAGTTTTTACCAATTGAAAGGGAAAAACCTAATGGGGAATCAAGACAAGATTTAGTGGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTCGTCGTCACCACTTACGCGGGGCTCTACCGTTATTGCATGGGTGACATTCTTCGTGTAACAGGGTTTAAAAATAAAGCCCCGAACTTTAGTTTTGTATGTAGGAGGAACATAATTCTTAGCCTGGATTTAGAGAAAACAGAGGAAGGTGAGCTTCACAATGCTATACAAGAAGGTGCTCGTCTTTTAGGTCCATTTGGAGCTGTTGTTGCTGACTACACAAGCTATGCAGATAACAGAACAATTCCTGGACACTATGTATTGTATTGGGAAATTCGTATTAAGGAAGAAAGTTCCATTATCCCATCTTCGATCTATGAGGATTGTTGCCCCAAATGGGATCCCAGTAGTCGACACCATCTTTTTTCTGCTTAGTTGATGAGAGAAAATTAATTATCTAGCAAGTGTTTTTAAAGTTGTTGTGATGCATTTGCAGTATTGTTTGTTGTGGTTACTTTTGTGTAATAGATGACTTTAACCTCTCATTTTCGTTTATTTGGTTTTTTTTACTCTTGATATGGTCAAACAAAATCTTATCATAAAGTGGTTTACA

Coding sequence (CDS)

ATGTCGAATGATTCAGAAAACCTGTTGGAGGAGAAAGATAAGCAAACATTACAACATATGGAAGATATCACGAGCAACGCCGACGAAATCCAACGACAAATTCTAGTCGAAATTTTGTCAACAAATGGCCATGTTGAGTACTTGCAGCAACATGGCCTTAACGGATCTACTGATGCTTCAACTTTCAAGAACCTTATACCTGTCGTATCCTATGAACAACTGAAACCTTATATAGATCGTATTGCGTACGGTGATGATTCTCCAATCCTATGCGCACAACCCATCACACAGTTCTTTATCAGCTCTGGAACATCGGGGGGAGAACAAAAGCTAATTCCAATGTTTGAACAAGAAGTTGAGAGAATTTTCTCTCCTATAATGAAGTATGAGATCCCAAAGATGAAACAATTGTTTCCAAATTCGCACAAAGGCAAAGAAATGTTCTTTCTTTTCAACAGGCCTCAGGCAAGAACTAAATCAGGAATTATTGCTCAATCTGTATCTACAAGTTTTTGCAAGAAACCACACTTATTTAACAATATACTGACTAGAGGTTGCACAAGCCCTAACGGCGTCCTTTTATGCACAGACACATACCAAAGCACATATTCTCAGTTACTTTGTGGGCTCTACCAAAACGAGTCAGTTTTTATATTGGGTTCTCTTTTTATCTCCACTCTAGCTTATGTTCTTAAGTTCCTCGAAAATCATTGGATTCTTTTGGCTAATGATATACGAACAGGAATCATAAACTCCAAAATTACAGATTCATTAGTGAGAGAATCAGTTATGAATATTCTTGAACCTAATCCCCACCTTGCAGATTTTATTGAAACAGAATGTAGCAAAGGAACATGGGAAGGAATCATAACAAGATTGTGGCCAAATGCCAAATACATTAAAGCTATTGCGACAGGAAGCATGTCACAATACATTCTCCAGCTAAATTACTACAGCAACAACCTCCCTCTTTGCTTCCTTGGCTTAAACCTAGATCCATTATGCAAACCAGAGGAAATCAGCTACACTTTAATCCCCACTATGGCATATTTTGAGTTTTTACCAATTGAAAGGGAAAAACCTAATGGGGAATCAAGACAAGATTTAGTGGATCTTGTGGATGTGAAGTTAGGACAAGAATATGAGCTCGTCGTCACCACTTACGCGGGGCTCTACCGTTATTGCATGGGTGACATTCTTCGTGTAACAGGGTTTAAAAATAAAGCCCCGAACTTTAGTTTTGTATGTAGGAGGAACATAATTCTTAGCCTGGATTTAGAGAAAACAGAGGAAGGTGAGCTTCACAATGCTATACAAGAAGGTGCTCGTCTTTTAGGTCCATTTGGAGCTGTTGTTGCTGACTACACAAGCTATGCAGATAACAGAACAATTCCTGGACACTATGTATTGTATTGGGAAATTCGTATTAAGGAAGAAAGTTCCATTATCCCATCTTCGATCTATGAGGATTGTTGCCCCAAATGGGATCCCAGTAGTCGACACCATCTTTTTTCTGCTTAG

Protein sequence

MSNDSENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFSPIMKYEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILTRGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDIRTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIATGSMSQYILQLNYYSNNLPLCFLGLNLDPLCKPEEISYTLIPTMAYFEFLPIEREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYVLYWEIRIKEESSIIPSSIYEDCCPKWDPSSRHHLFSA

Homology

[Close]

BLAST of Sed0019920 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 723.4 bits (1866), Expect = 1.4e-204
Identity = 350/493 (70.99%), Postives = 400/493 (81.14%), Query Frame = 0


Query: 9   LEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPV 68
           L+EKDKQ LQ +ED+T  ADEIQR++L EILSTN HVEYLQQHGLNGSTDASTFKNLIP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  VSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFSPIMK 128
           VSYE LKPYIDRI  GDDSPILCA PIT+F+ S+GTSGGEQKLIP  EQE  R  S I  
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLS-ISN 123

Query: 129 YEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILTRGCTS 188
           Y  P+MKQLFP  H GKEMFF F + ++ TK+GI+A+SVSTSF K PHL N  +    TS
Sbjct: 124 YMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 183

Query: 189 PNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDIRTG 248
           P  +L CTD YQS Y QLLCGL QN+ V  +GSLF STL Y+ KFLENHW+LLANDIRTG
Sbjct: 184 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 243

Query: 249 IINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIATGSM 308
            IN+KI DS VR+SVMNIL+P+P LADFIE+ECSKGTWEGI+TRLWPN K+I A+ TGSM
Sbjct: 244 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 303

Query: 309 SQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFLPIERE 368
           S YI QLNYY  +LPL         CF G+NLDPLCKPEEISYT IPT+AYFEFLP++R 
Sbjct: 304 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 363

Query: 369 KPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFSFVCRRN 428
             +GE  Q+ VDLVDVKLGQEYE+VVTTYAGLYRYC+GDI+RVTGFKNKAPNF FV R+N
Sbjct: 364 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 423

Query: 429 IILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYVLYWEIRIKEE 488
           +ILSLD+EKT+E ELHNA+ +   LL  FGA++ DYTSYAD+ T PGHYVLYWEI+ KEE
Sbjct: 424 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 483

Query: 489 SSIIPSSIYEDCC 493
            SIIPSSIYEDCC
Sbjct: 484 ISIIPSSIYEDCC 495

[Close]

BLAST of Sed0019920 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 697.6 bits (1799), Expect = 8.0e-197
Identity = 344/503 (68.39%), Postives = 407/503 (80.91%), Query Frame = 0


Query: 1   MSNDSENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDAS 60
           M++  E   +E+DKQ LQH+ED+T +A +IQRQIL +ILSTN  VEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVE 120
           TFKNLIP++SYEQL+PYIDRIA GDDSPILC  PIT+FFISSGTSGGE KLIPM+E+E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RIFSPIMKYEIPKMKQLFPNS--HKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLF 180
           R  S    Y +P+MKQLFPNS   KGK M F F + + +T+SGI+A + +TS CK+ +L 
Sbjct: 121 RRLS-FFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN 180

Query: 181 NNILTRGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHW 240
           N       TSP  ++LCTDT QS Y QLLCGL QN+ VF +G+LF STL +VLKFLE+HW
Sbjct: 181 N-------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHW 240

Query: 241 ILLANDIRTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAK 300
           ++LA+DIR G +N+KITD  VRE+VMNIL PN  LADFIE ECSKGTWEGIITRLWPNAK
Sbjct: 241 VVLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAK 300

Query: 301 YIKAIATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMA 360
           YI AI TGSM+QY+ +LNYYSN+LP+         CFLGLNLDPLC P+  SYTLIPTMA
Sbjct: 301 YINAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMA 360

Query: 361 YFEFLPIEREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKA 420
           YFEFLPI+R K + E  ++LVDLVDVKLG EYELV+TTYAGLYRYCMGDILRVTGFKNKA
Sbjct: 361 YFEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKA 420

Query: 421 PNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYV 480
           PNFSFVCR+N++LSLD+EKT+E ELH+A+QE ARLL PFGA +ADYTS+AD+  IPGHYV
Sbjct: 421 PNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYV 480

Query: 481 LYWEIRIKEESSIIPSSIYEDCC 493
           LYWEI +K   S IPSSIYEDCC
Sbjct: 481 LYWEIHVKGGIS-IPSSIYEDCC 494

[Close]

BLAST of Sed0019920 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 690.3 bits (1780), Expect = 1.3e-194
Identity = 342/503 (67.99%), Postives = 405/503 (80.52%), Query Frame = 0


Query: 1   MSNDSENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDAS 60
           M++  E   +E+DKQ LQH+ED+T +A +IQRQIL +ILSTN  VEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVE 120
           TFKNLIP++SYEQL+PYIDRIA GDDSPILC  PIT+FFISSGTSGGE KLIPM+E+E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RIFSPIMKYEIPKMKQLFPNS--HKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLF 180
           R  S    Y +P+MKQLFPNS   KGK M F F + + +T+SGI+A + +TS CK+ +L 
Sbjct: 121 RRLS-FFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN 180

Query: 181 NNILTRGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHW 240
           N       TSP  ++LCTDT QS Y QLLCGL QN+ VF +G+LF STL +VLKFLE+HW
Sbjct: 181 N-------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHW 240

Query: 241 ILLANDIRTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAK 300
           ++LA+DIR G +N+KITD  VRE+VMNIL PN  LADFIE ECSKGTWEGIITRLWPNAK
Sbjct: 241 VVLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAK 300

Query: 301 YIKAIATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMA 360
           YI AI TGSM+QY+ +LNYYSN+LP+         CFLGLNLDPLC P+  SYTLIPTMA
Sbjct: 301 YINAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMA 360

Query: 361 YFEFLPIEREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKA 420
           YFEFLPI+R K + E  ++LVDLVDVKLG EYELV+TTYAGLYRY MGDIL VTGFKNKA
Sbjct: 361 YFEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKA 420

Query: 421 PNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYV 480
           PNFSFVCR+N++LSLD+EKT+E ELH+A+QE ARLL PFGA +ADYTS+AD+  IPGHYV
Sbjct: 421 PNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYV 480

Query: 481 LYWEIRIKEESSIIPSSIYEDCC 493
           LYWEI +K   S IPSSIYEDCC
Sbjct: 481 LYWEIHVKGGIS-IPSSIYEDCC 494

[Close]

BLAST of Sed0019920 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 675.6 bits (1742), Expect = 3.3e-190
Identity = 332/499 (66.53%), Postives = 394/499 (78.96%), Query Frame = 0


Query: 5   SENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKN 64
           S+++L+ KDKQ L H+ED TSNADEIQR+++ EILS N HVEYL+Q GL+GSTD STFK 
Sbjct: 2   SDDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKK 61

Query: 65  LIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFS 124
           LIPVVSYEQLKPYIDRIA GDDSPILCA  IT F IS+GTSGG QKLIPMF+ E+ R  +
Sbjct: 62  LIPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLA 121

Query: 125 PIMKYEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNI-LT 184
               Y IP MKQLFP    GKEMFF F R + +TK G++AQ +S S   K  LF+++ + 
Sbjct: 122 -TNYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLS-SCLSKCSLFSSVEVL 181

Query: 185 RGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLAN 244
           R  TSP  ++ CTD YQS Y QLLCGL QN+ V  +G+LF STL Y+ KFLENHW LL N
Sbjct: 182 RAYTSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVN 241

Query: 245 DIRTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAI 304
           DIRTG +++KI D LVRESVM++L+PNP LADFIE+ECSKG WEGI+TRLWPN KYI  +
Sbjct: 242 DIRTGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTV 301

Query: 305 ATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFL 364
            TGSMSQYI QLN+Y    P+         CF G+NLDPLCKPEEISYT +PTMAYFEFL
Sbjct: 302 VTGSMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFL 361

Query: 365 PIER-EKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFS 424
           P++R    NGE  Q+LVDLVDVKLGQEYE+VVTTYAGLYRYCMGDILRVTGFKNK PNFS
Sbjct: 362 PVDRANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFS 421

Query: 425 FVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYVLYWE 484
           F CR+N+ILSLD+EKT+E +LH+A+ E + LL P GA++ DYTSYAD+ T PGHYVLYWE
Sbjct: 422 FACRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWE 481

Query: 485 IRIKEESSIIPSSIYEDCC 493
           I+ +EE+S IPS++YEDCC
Sbjct: 482 IQCREETSTIPSTVYEDCC 497

[Close]

BLAST of Sed0019920 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 668.7 bits (1724), Expect = 4.0e-188
Identity = 336/509 (66.01%), Postives = 398/509 (78.19%), Query Frame = 0


Query: 1   MSNDSENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDAS 60
           MS+  E+ L+EKDK+ LQH+ED+TS ADEIQRQIL EILSTN +VEYLQQHGL+GSTDAS
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 61  TFKNLIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVE 120
           TFK LIP++SYEQL PY+  IA+ DDSPILC+ PIT FF SSGTSGGE KLIPM+E+E  
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 121 RIFSPIMKYEIPKMKQLFPNSH--KGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLF 180
           R  S    Y +P+MKQLFPN +  KGK M F F +P+ +TK+GII +S+ TS  K+    
Sbjct: 121 RRLS-FFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRSSNL 180

Query: 181 NNILTRGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHW 240
            +      TSP+ ++LCTD++QS Y QLLCGLYQN+ VF LG+LF STL +V KFLENHW
Sbjct: 181 KSKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENHW 240

Query: 241 ILLANDIRTGIINSKITDSLVRESVMNI-LEPNPHLADFIETECSKGTWEGIITRLWPNA 300
           ++LANDI TG IN KIT+  VRESVM I ++PNP +AD IE ECSKG+WEGII RLWPN 
Sbjct: 241 VVLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNT 300

Query: 301 KYIKAIATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTM 360
           KYI AI TGSMSQYI  LN+Y+NNLP+         CFLGLNLDPLC P EISYTLIPTM
Sbjct: 301 KYINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTM 360

Query: 361 AYFEFLPIEREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNK 420
           AYFEFLPI+R  PNGE  Q LVDLVDVKLGQEYELV+TT+AGLYRY MGDI+RVTGF  K
Sbjct: 361 AYFEFLPIDRTNPNGEVTQQLVDLVDVKLGQEYELVITTFAGLYRYRMGDIIRVTGFTKK 420

Query: 421 APNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHY 480
           APNFSFVCR+N++LSL+ EKT+E  LH A++EG  +L PFGA + DYTSYAD+ TIPGHY
Sbjct: 421 APNFSFVCRKNVVLSLNNEKTDEAGLHKAVEEGGHVLKPFGATIVDYTSYADSSTIPGHY 480

Query: 481 VLYWEIRIKEE-----SSIIPSSIYEDCC 493
           VLY+E+   E      S+ IPSS+YEDCC
Sbjct: 481 VLYFELIFDEHNKAQISNSIPSSVYEDCC 508

[Close]

BLAST of Sed0019920 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.7e-157
Identity = 276/508 (54.33%), Postives = 366/508 (72.05%), Query Frame = 0


Query: 9   LEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPV 68
           L++K+KQ LQ +E++TSNAD++QRQ+L EIL+ N  VEYL++H LNG TD  TFKN++PV
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  VSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFSPIMK 128
           ++YE ++P I+RIA GD SPIL ++PI++F  SSGTSGGE+KL+P  E+E++R  S +  
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDR-RSLLYS 136

Query: 129 YEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILT--RGC 188
             +P M Q  P    GK M+FLF + +++T  G+ A+ V TS+ K  H            
Sbjct: 137 LLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 196

Query: 189 TSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDIR 248
           TSPN  +LC+D+YQS YSQ+LCGL Q++ V  +G++F S     +KFLE HWI L  DIR
Sbjct: 197 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 256

Query: 249 TGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIATG 308
           TG ++S ITD  VRE+V  IL+P+P LADF+E EC K +W+GIITRLWPN KY+  I TG
Sbjct: 257 TGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTG 316

Query: 309 SMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFLPIE 368
           +MSQYI  L+YYSN LPL         C+ G+NL PLCKP E+SYTLIP+MAYFEFLP+ 
Sbjct: 317 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVH 376

Query: 369 REKPNG-------------ESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTG 428
           R   NG             + +Q+LVDLVDVKLGQEYELVVTTYAGL RY +GD+LRVTG
Sbjct: 377 RN--NGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTG 436

Query: 429 FKNKAPNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTI 488
           FKNKAP FSF+CR+N++LS+D +KT+E EL NA++     L PF A +++YTSYAD  +I
Sbjct: 437 FKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSI 496

Query: 489 PGHYVLYWEIRIKEESSIIPSSIYEDCC 493
           PGHYVL+WE+ + + ++ IP S++EDCC
Sbjct: 497 PGHYVLFWELCL-DGNTPIPPSVFEDCC 520

[Close]

BLAST of Sed0019920 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.2e-157
Identity = 275/510 (53.92%), Postives = 363/510 (71.18%), Query Frame = 0


Query: 5   SENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKN 64
           S+  L EK+K  LQ +ED+T+NAD++QR++L EILS N  VEYL++HGL G TD  TFK+
Sbjct: 13  SDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKH 72

Query: 65  LIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFS 124
           ++PVV+YE ++P I+RIA GD S +LC+ PI++F  SSGTSGGE+KL+P  E+E++R  S
Sbjct: 73  IMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDR-RS 132

Query: 125 PIMKYEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILT- 184
            +    +P M Q  P   KGK M+FLF + +++T  G+ A+ V TS+ K  H  N     
Sbjct: 133 LLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDP 192

Query: 185 -RGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLA 244
               TSPN  +LC+D+YQS YSQ+LCGL Q++ V  +G++F S     +KFLE HW  LA
Sbjct: 193 YTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELA 252

Query: 245 NDIRTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKA 304
            DIRTG ++S+ITDS VRE+V  IL+P+P LADF+E+EC K +W+GIITRLWPN KY+  
Sbjct: 253 RDIRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDV 312

Query: 305 IATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEF 364
           I TG+MSQYI  L+YYSN LPL         C+ G+NL PLCKP E+SYTLIP MAYFEF
Sbjct: 313 IVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEF 372

Query: 365 LPIEREKP-----------NGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRV 424
           LP+ R                + +Q+LVDLVDVKLGQEYELVVTTYAGLYRY +GD+L V
Sbjct: 373 LPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSV 432

Query: 425 TGFKNKAPNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNR 484
            GFKN AP FSF+CR+N++LS+D +KT+E EL NA++     L PF A +++YTSYAD  
Sbjct: 433 AGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTS 492

Query: 485 TIPGHYVLYWEIRIKEESSIIPSSIYEDCC 493
           +IPGHYVL+WE+ +   +  IP S++EDCC
Sbjct: 493 SIPGHYVLFWELCLNGNTP-IPPSVFEDCC 520

[Close]

BLAST of Sed0019920 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 2.8e-136
Identity = 244/491 (49.69%), Postives = 331/491 (67.41%), Query Frame = 0


Query: 15  QTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTD-ASTFKNLIPVVSYEQ 74
           + L+ +E +T+NA ++QR++L EIL+ N   EYL+++G+ GS D    F+ L+P+V+YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 75  LKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFSPIMKYEIPK 134
           L+P I RIA GD SPI   +PI++F  SSGTSGGE+KL+P    E+ R  S +    +P 
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNR-RSLLYSLLMPV 141

Query: 135 MKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILT--RGCTSPNG 194
           M Q      KGK M+ LF + ++RT  G+ A+ V TS+ +     +         TSP+ 
Sbjct: 142 MSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDE 201

Query: 195 VLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDIRTGIIN 254
            +LC D+YQS Y+QLLCGL     V  +G++F S     + FLE HW  L +DIRTG ++
Sbjct: 202 AILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELD 261

Query: 255 SKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIATGSMSQY 314
            +ITD +VR++V  +L  +P LAD IE EC++ +WEGII RLWP  KYI  I TG+MSQY
Sbjct: 262 PEITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQY 321

Query: 315 ILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFLPIEREKPN 374
           I  L +Y   LPL         C+ GLNL+P+CKP +++YTLIPTM Y+EFLP+      
Sbjct: 322 IPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNAT 381

Query: 375 GE-SRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFSFVCRRNII 434
            E S +DLVDLVDVKLG EYELVVTTY+GLYRY +GD+LRV GFKNKAP FSFV R+N+ 
Sbjct: 382 AEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVA 441

Query: 435 LSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYVLYWEIRIKEESS 493
           LS+D +KT+E ELH A+    + L PFGA + +YTSYAD  TIPGHYVL+WE+R    S+
Sbjct: 442 LSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELR--AGST 501

[Close]

BLAST of Sed0019920 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 4.3e-129
Identity = 239/503 (47.51%), Postives = 328/503 (65.21%), Query Frame = 0


Query: 14  KQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDAS--TFKNLIPVVSY 73
           ++ L+H+E +T NA + QR++L EIL+ N   EYL++ G+ G    +   F+ L P+V+Y
Sbjct: 24  REKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLVTY 83

Query: 74  EQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFSPIMKYEI 133
           E + P + RIA GD SPIL  +P+++F  SSGTSGGE+KL+P  E+E+ER  S +    +
Sbjct: 84  EDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMER-RSGLYSLLM 143

Query: 134 PKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTS------FCKKPHLFNNILTRG 193
           P M +  P   KGK M+  F + + RT  G+ A+ V TS      F ++PH    +    
Sbjct: 144 PVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVY--- 203

Query: 194 CTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDI 253
            TSP+  +LC D YQS Y+QL+CGL     V  +G++F S     ++FLE HW  L  DI
Sbjct: 204 -TSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDI 263

Query: 254 RTGIINSKITDSLVRESVMNILE-PNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIA 313
           R G ++  +TD  VR +V  +L   +P LAD IE EC++ +W+GII R+WP+ KYI  I 
Sbjct: 264 RAGELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIV 323

Query: 314 TGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFLP 373
           TG+M+QYI  L +Y   LPL         C+ GLNL+P+CKP E++YTLIPTM YFEFLP
Sbjct: 324 TGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLP 383

Query: 374 I-----EREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAP 433
           +     +   P  + R  LVDLVDVKLG EYELVVTTY+GLYRY +GD+LRV GFKN AP
Sbjct: 384 VNSGANDVAAPEPDHR-GLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAP 443

Query: 434 NFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADN-RTIPGHYV 493
            F+FV R+N+ LS+D +KT+E ELH A+ E  + L PFGA + +YTSYAD   TIPGHYV
Sbjct: 444 MFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYV 503

[Close]

BLAST of Sed0019920 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 7.4e-129
Identity = 236/497 (47.48%), Postives = 324/497 (65.19%), Query Frame = 0


Query: 11  EKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPVVS 70
           EKD + L+ +E++T NAD +Q  +L EIL+ N   EYL++  L G+TD  TFK  IPV++
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  YEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVER---IFSPIM 130
           YE L+P I RIA GD SPIL A PI++F  SSGTS GE+KL+P  ++E++R   ++S +M
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 KYEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILT--RG 190
               P M    P   KGK M+FLF + + +T  G+ A+ V TS+ K  H  +        
Sbjct: 137 ----PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNV 196

Query: 191 CTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDI 250
            TSPN  +LC D++QS Y+Q+LCGL    SV  +G++F S L   ++FL+ HW   A+DI
Sbjct: 197 YTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDI 256

Query: 251 RTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIAT 310
             G ++S+ITD  +R+ +  IL+P+P LA+FI  EC    WE IITR+WPN KY+  I T
Sbjct: 257 ELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVT 316

Query: 311 GSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFLPI 370
           G+M+QYI  L YYS  LP+         C+ GLNL+P+ KP E+SYT++P MAYFEF+P+
Sbjct: 317 GAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPL 376

Query: 371 EREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFSFVC 430
              K         V+LVDV +G+EYELVVTTYAGL RY +GDILRVTGF N AP F FV 
Sbjct: 377 GGTK--------AVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVR 436

Query: 431 RRNIILSLDLEKTEEGELHNAIQEGARLL-GPFGAVVADYTSYADNRTIPGHYVLYWEIR 490
           R+N++LS+D +KT+E EL  A++  + +L    G+ VA+YTSYAD  TIPGHYVLYWE+ 
Sbjct: 437 RKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELL 496

Query: 491 IKEESSIIPSSIYEDCC 493
           +++ +          CC
Sbjct: 497 VRDGARQPSHETLTRCC 501

[Close]

BLAST of Sed0019920 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 6.6e-205
Identity = 350/493 (70.99%), Postives = 400/493 (81.14%), Query Frame = 0


Query: 9   LEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPV 68
           L+EKDKQ LQ +ED+T  ADEIQR++L EILSTN HVEYLQQHGLNGSTDASTFKNLIP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  VSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFSPIMK 128
           VSYE LKPYIDRI  GDDSPILCA PIT+F+ S+GTSGGEQKLIP  EQE  R  S I  
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLS-ISN 123

Query: 129 YEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILTRGCTS 188
           Y  P+MKQLFP  H GKEMFF F + ++ TK+GI+A+SVSTSF K PHL N  +    TS
Sbjct: 124 YMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 183

Query: 189 PNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDIRTG 248
           P  +L CTD YQS Y QLLCGL QN+ V  +GSLF STL Y+ KFLENHW+LLANDIRTG
Sbjct: 184 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 243

Query: 249 IINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIATGSM 308
            IN+KI DS VR+SVMNIL+P+P LADFIE+ECSKGTWEGI+TRLWPN K+I A+ TGSM
Sbjct: 244 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 303

Query: 309 SQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFLPIERE 368
           S YI QLNYY  +LPL         CF G+NLDPLCKPEEISYT IPT+AYFEFLP++R 
Sbjct: 304 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 363

Query: 369 KPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFSFVCRRN 428
             +GE  Q+ VDLVDVKLGQEYE+VVTTYAGLYRYC+GDI+RVTGFKNKAPNF FV R+N
Sbjct: 364 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 423

Query: 429 IILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYVLYWEIRIKEE 488
           +ILSLD+EKT+E ELHNA+ +   LL  FGA++ DYTSYAD+ T PGHYVLYWEI+ KEE
Sbjct: 424 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 483

Query: 489 SSIIPSSIYEDCC 493
            SIIPSSIYEDCC
Sbjct: 484 ISIIPSSIYEDCC 495

[Close]

BLAST of Sed0019920 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 6.2e-195
Identity = 342/503 (67.99%), Postives = 405/503 (80.52%), Query Frame = 0


Query: 1   MSNDSENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDAS 60
           M++  E   +E+DKQ LQH+ED+T +A +IQRQIL +ILSTN  VEYLQQHGL+GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKNLIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVE 120
           TFKNLIP++SYEQL+PYIDRIA GDDSPILC  PIT+FFISSGTSGGE KLIPM+E+E  
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RIFSPIMKYEIPKMKQLFPNS--HKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLF 180
           R  S    Y +P+MKQLFPNS   KGK M F F + + +T+SGI+A + +TS CK+ +L 
Sbjct: 121 RRLS-FFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLN 180

Query: 181 NNILTRGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHW 240
           N       TSP  ++LCTDT QS Y QLLCGL QN+ VF +G+LF STL +VLKFLE+HW
Sbjct: 181 N-------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHW 240

Query: 241 ILLANDIRTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAK 300
           ++LA+DIR G +N+KITD  VRE+VMNIL PN  LADFIE ECSKGTWEGIITRLWPNAK
Sbjct: 241 VVLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAK 300

Query: 301 YIKAIATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMA 360
           YI AI TGSM+QY+ +LNYYSN+LP+         CFLGLNLDPLC P+  SYTLIPTMA
Sbjct: 301 YINAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMA 360

Query: 361 YFEFLPIEREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKA 420
           YFEFLPI+R K + E  ++LVDLVDVKLG EYELV+TTYAGLYRY MGDIL VTGFKNKA
Sbjct: 361 YFEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKA 420

Query: 421 PNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYV 480
           PNFSFVCR+N++LSLD+EKT+E ELH+A+QE ARLL PFGA +ADYTS+AD+  IPGHYV
Sbjct: 421 PNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYV 480

Query: 481 LYWEIRIKEESSIIPSSIYEDCC 493
           LYWEI +K   S IPSSIYEDCC
Sbjct: 481 LYWEIHVKGGIS-IPSSIYEDCC 494

[Close]

BLAST of Sed0019920 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.6e-190
Identity = 332/499 (66.53%), Postives = 394/499 (78.96%), Query Frame = 0


Query: 5   SENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKN 64
           S+++L+ KDKQ L H+ED TSNADEIQR+++ EILS N HVEYL+Q GL+GSTD STFK 
Sbjct: 2   SDDILDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKK 61

Query: 65  LIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFS 124
           LIPVVSYEQLKPYIDRIA GDDSPILCA  IT F IS+GTSGG QKLIPMF+ E+ R  +
Sbjct: 62  LIPVVSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLA 121

Query: 125 PIMKYEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNI-LT 184
               Y IP MKQLFP    GKEMFF F R + +TK G++AQ +S S   K  LF+++ + 
Sbjct: 122 -TNYYAIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLS-SCLSKCSLFSSVEVL 181

Query: 185 RGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLAN 244
           R  TSP  ++ CTD YQS Y QLLCGL QN+ V  +G+LF STL Y+ KFLENHW LL N
Sbjct: 182 RAYTSPIDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVN 241

Query: 245 DIRTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAI 304
           DIRTG +++KI D LVRESVM++L+PNP LADFIE+ECSKG WEGI+TRLWPN KYI  +
Sbjct: 242 DIRTGTVDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTV 301

Query: 305 ATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFL 364
            TGSMSQYI QLN+Y    P+         CF G+NLDPLCKPEEISYT +PTMAYFEFL
Sbjct: 302 VTGSMSQYIPQLNFYC-KCPIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFL 361

Query: 365 PIER-EKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFS 424
           P++R    NGE  Q+LVDLVDVKLGQEYE+VVTTYAGLYRYCMGDILRVTGFKNK PNFS
Sbjct: 362 PVDRANSNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFS 421

Query: 425 FVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYVLYWE 484
           F CR+N+ILSLD+EKT+E +LH+A+ E + LL P GA++ DYTSYAD+ T PGHYVLYWE
Sbjct: 422 FACRKNVILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWE 481

Query: 485 IRIKEESSIIPSSIYEDCC 493
           I+ +EE+S IPS++YEDCC
Sbjct: 482 IQCREETSTIPSTVYEDCC 497

[Close]

BLAST of Sed0019920 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 1.0e-181
Identity = 323/508 (63.58%), Postives = 391/508 (76.97%), Query Frame = 0


Query: 1   MSNDSENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDAS 60
           MSN  E  L+EKDK+ LQ++EDIT+ ADEIQRQIL EILS N +VEYLQ+HGL+ STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFKNLIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVE 120
           TFK LIP+V Y+QL+PYI RIA GDDS ILC+ PIT+FF SSGTSG E KLIPM+EQE  
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 RIFSPIMKYEIPKMKQLFPNS--HKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLF 180
           R  S    Y  P+MKQLFPN   HK K + F F +P+ +TK GI+ +S+ T+  K+    
Sbjct: 121 RRLS-FFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNL 180

Query: 181 NNILTRGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHW 240
            ++ +   TSP+ ++LCTD+YQS Y QLLCGLYQN+ VF + +LF STL +V KFLENHW
Sbjct: 181 RSMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHW 240

Query: 241 ILLANDIRTGIINSKITDSLVRESVMNI-LEPNPHLADFIETECSKGTWEGIITRLWPNA 300
           I LA DIRT  IN KIT S VRESVM I ++PNP +AD IE EC KG WEGIITRLWPNA
Sbjct: 241 IDLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNA 300

Query: 301 KYIKAIATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTM 360
           KYI A+ TGSMSQYI  LNYY+NNLP+         CFLGLNLDPLC P+E+SYTLIPTM
Sbjct: 301 KYINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTM 360

Query: 361 AYFEFLPIEREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNK 420
           AYFEFLPI+   P GE  Q LVDLVDVKLG EYELV+TT+AGLYRYCMGD++RVTGFKNK
Sbjct: 361 AYFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNK 420

Query: 421 APNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHY 480
           APNFSFV R+N++L++  EKT+E  LH A++EG R++  FGA + DYT+YAD  TIPGHY
Sbjct: 421 APNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHY 480

Query: 481 VLYWEIR---IKEES-SIIPSSIYEDCC 493
           VLYWE+    +KE++ + IP S++EDCC
Sbjct: 481 VLYWELTLDDLKEQNLNYIPCSVFEDCC 507

[Close]

BLAST of Sed0019920 vs. ExPASy TrEMBL
Match: A0A1S3BJT8 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC103490488 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 1.0e-181
Identity = 323/508 (63.58%), Postives = 391/508 (76.97%), Query Frame = 0


Query: 1   MSNDSENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDAS 60
           MSN  E  L+EKDK+ LQ++EDIT+ ADEIQRQIL EILS N +VEYLQ+HGL+ STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFKNLIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVE 120
           TFK LIP+V Y+QL+PYI RIA GDDS ILC+ PIT+FF SSGTSG E KLIPM+EQE  
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 RIFSPIMKYEIPKMKQLFPNS--HKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLF 180
           R  S    Y  P+MKQLFPN   HK K + F F +P+ +TK GI+ +S+ T+  K+    
Sbjct: 121 RRLS-FFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNL 180

Query: 181 NNILTRGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHW 240
            ++ +   TSP+ ++LCTD+YQS Y QLLCGLYQN+ VF + +LF STL +V KFLENHW
Sbjct: 181 RSMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHW 240

Query: 241 ILLANDIRTGIINSKITDSLVRESVMNI-LEPNPHLADFIETECSKGTWEGIITRLWPNA 300
           I LA DIRT  IN KIT S VRESVM I ++PNP +AD IE EC KG WEGIITRLWPNA
Sbjct: 241 IDLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNA 300

Query: 301 KYIKAIATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTM 360
           KYI A+ TGSMSQYI  LNYY+NNLP+         CFLGLNLDPLC P+E+SYTLIPTM
Sbjct: 301 KYINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTM 360

Query: 361 AYFEFLPIEREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNK 420
           AYFEFLPI+   P GE  Q LVDLVDVKLG EYELV+TT+AGLYRYCMGD++RVTGFKNK
Sbjct: 361 AYFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNK 420

Query: 421 APNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHY 480
           APNFSFV R+N++L++  EKT+E  LH A++EG R++  FGA + DYT+YAD  TIPGHY
Sbjct: 421 APNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHY 480

Query: 481 VLYWEIR---IKEES-SIIPSSIYEDCC 493
           VLYWE+    +KE++ + IP S++EDCC
Sbjct: 481 VLYWELTLDDLKEQNLNYIPCSVFEDCC 507

[Close]

BLAST of Sed0019920 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 557.4 bits (1435), Expect = 1.2e-158
Identity = 276/508 (54.33%), Postives = 366/508 (72.05%), Query Frame = 0


Query: 9   LEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPV 68
           L++K+KQ LQ +E++TSNAD++QRQ+L EIL+ N  VEYL++H LNG TD  TFKN++PV
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  VSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFSPIMK 128
           ++YE ++P I+RIA GD SPIL ++PI++F  SSGTSGGE+KL+P  E+E++R  S +  
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDR-RSLLYS 136

Query: 129 YEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILT--RGC 188
             +P M Q  P    GK M+FLF + +++T  G+ A+ V TS+ K  H            
Sbjct: 137 LLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 196

Query: 189 TSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDIR 248
           TSPN  +LC+D+YQS YSQ+LCGL Q++ V  +G++F S     +KFLE HWI L  DIR
Sbjct: 197 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 256

Query: 249 TGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIATG 308
           TG ++S ITD  VRE+V  IL+P+P LADF+E EC K +W+GIITRLWPN KY+  I TG
Sbjct: 257 TGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTG 316

Query: 309 SMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFLPIE 368
           +MSQYI  L+YYSN LPL         C+ G+NL PLCKP E+SYTLIP+MAYFEFLP+ 
Sbjct: 317 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVH 376

Query: 369 REKPNG-------------ESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTG 428
           R   NG             + +Q+LVDLVDVKLGQEYELVVTTYAGL RY +GD+LRVTG
Sbjct: 377 RN--NGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTG 436

Query: 429 FKNKAPNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTI 488
           FKNKAP FSF+CR+N++LS+D +KT+E EL NA++     L PF A +++YTSYAD  +I
Sbjct: 437 FKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSI 496

Query: 489 PGHYVLYWEIRIKEESSIIPSSIYEDCC 493
           PGHYVL+WE+ + + ++ IP S++EDCC
Sbjct: 497 PGHYVLFWELCL-DGNTPIPPSVFEDCC 520

[Close]

BLAST of Sed0019920 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 557.0 bits (1434), Expect = 1.6e-158
Identity = 275/510 (53.92%), Postives = 363/510 (71.18%), Query Frame = 0


Query: 5   SENLLEEKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKN 64
           S+  L EK+K  LQ +ED+T+NAD++QR++L EILS N  VEYL++HGL G TD  TFK+
Sbjct: 13  SDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKH 72

Query: 65  LIPVVSYEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFS 124
           ++PVV+YE ++P I+RIA GD S +LC+ PI++F  SSGTSGGE+KL+P  E+E++R  S
Sbjct: 73  IMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDR-RS 132

Query: 125 PIMKYEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILT- 184
            +    +P M Q  P   KGK M+FLF + +++T  G+ A+ V TS+ K  H  N     
Sbjct: 133 LLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDP 192

Query: 185 -RGCTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLA 244
               TSPN  +LC+D+YQS YSQ+LCGL Q++ V  +G++F S     +KFLE HW  LA
Sbjct: 193 YTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELA 252

Query: 245 NDIRTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKA 304
            DIRTG ++S+ITDS VRE+V  IL+P+P LADF+E+EC K +W+GIITRLWPN KY+  
Sbjct: 253 RDIRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDV 312

Query: 305 IATGSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEF 364
           I TG+MSQYI  L+YYSN LPL         C+ G+NL PLCKP E+SYTLIP MAYFEF
Sbjct: 313 IVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEF 372

Query: 365 LPIEREKP-----------NGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRV 424
           LP+ R                + +Q+LVDLVDVKLGQEYELVVTTYAGLYRY +GD+L V
Sbjct: 373 LPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSV 432

Query: 425 TGFKNKAPNFSFVCRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNR 484
            GFKN AP FSF+CR+N++LS+D +KT+E EL NA++     L PF A +++YTSYAD  
Sbjct: 433 AGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTS 492

Query: 485 TIPGHYVLYWEIRIKEESSIIPSSIYEDCC 493
           +IPGHYVL+WE+ +   +  IP S++EDCC
Sbjct: 493 SIPGHYVLFWELCLNGNTP-IPPSVFEDCC 520

[Close]

BLAST of Sed0019920 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 462.2 bits (1188), Expect = 5.3e-130
Identity = 236/497 (47.48%), Postives = 324/497 (65.19%), Query Frame = 0


Query: 11  EKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPVVS 70
           EKD + L+ +E++T NAD +Q  +L EIL+ N   EYL++  L G+TD  TFK  IPV++
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  YEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVER---IFSPIM 130
           YE L+P I RIA GD SPIL A PI++F  SSGTS GE+KL+P  ++E++R   ++S +M
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 KYEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILT--RG 190
               P M    P   KGK M+FLF + + +T  G+ A+ V TS+ K  H  +        
Sbjct: 137 ----PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNV 196

Query: 191 CTSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDI 250
            TSPN  +LC D++QS Y+Q+LCGL    SV  +G++F S L   ++FL+ HW   A+DI
Sbjct: 197 YTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDI 256

Query: 251 RTGIINSKITDSLVRESVMNILEPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIAT 310
             G ++S+ITD  +R+ +  IL+P+P LA+FI  EC    WE IITR+WPN KY+  I T
Sbjct: 257 ELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVT 316

Query: 311 GSMSQYILQLNYYSNNLPL---------CFLGLNLDPLCKPEEISYTLIPTMAYFEFLPI 370
           G+M+QYI  L YYS  LP+         C+ GLNL+P+ KP E+SYT++P MAYFEF+P+
Sbjct: 317 GAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPL 376

Query: 371 EREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFSFVC 430
              K         V+LVDV +G+EYELVVTTYAGL RY +GDILRVTGF N AP F FV 
Sbjct: 377 GGTK--------AVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVR 436

Query: 431 RRNIILSLDLEKTEEGELHNAIQEGARLL-GPFGAVVADYTSYADNRTIPGHYVLYWEIR 490
           R+N++LS+D +KT+E EL  A++  + +L    G+ VA+YTSYAD  TIPGHYVLYWE+ 
Sbjct: 437 RKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELL 496

Query: 491 IKEESSIIPSSIYEDCC 493
           +++ +          CC
Sbjct: 497 VRDGARQPSHETLTRCC 501

[Close]

BLAST of Sed0019920 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 458.4 bits (1178), Expect = 7.6e-129
Identity = 226/499 (45.29%), Postives = 324/499 (64.93%), Query Frame = 0


Query: 11  EKDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPVVS 70
           E + + L+ +E+IT N D +Q ++L EILS N + EYL++  LNG+ D  +FK+ +PVV 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 71  YEQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVERIFSPIMKYE 130
           YE LK  I RI+ GD SPIL + PIT+F  SSGTS GE+KL+P  E+++ R    +    
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINR-RQLLGNLL 137

Query: 131 IPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFNNILTRGCTSPN 190
           +P M    P   KGK ++FLF + ++ T  G+ A+   TS+ K  +   +      TSP 
Sbjct: 138 MPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPK 197

Query: 191 GVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDIRTGII 250
             +LC D+ QS Y+Q+LCGL     V  LG++F S L   + FL+N+W  L+ DI TG +
Sbjct: 198 EAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTL 257

Query: 251 NSKITDSLVRESVMNIL-EPNPHLADFIETECSKGTWEGIITRLWPNAKYIKAIATGSMS 310
           +SKI D  ++  + NIL +P+  LA+F+   CS+  WEGIIT++WPN KY+  I TG+M+
Sbjct: 258 SSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMA 317

Query: 311 QYILQLNYYSNNLPLC---------FLGLNLDPLCKPEEISYTLIPTMAYFEFLPIEREK 370
           +YI  L YYS  LP+          + G+NL+P+CKP E+SYT+ P MAYFEFLP   + 
Sbjct: 318 EYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDG 377

Query: 371 PNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFSFVCRRNI 430
             G     LV+L DV++G+EYELV+TTYAGLYRY +GDILRVTGF N AP F F+ R N+
Sbjct: 378 DGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENV 437

Query: 431 ILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYVLYWEIRIKEES 490
           +LS++ +KT+E +L  A++  +RLL   G  V +YTSYAD +TIPGHYV+YWE+  +++S
Sbjct: 438 LLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQS 497

Query: 491 SIIPS-SIYEDCCPKWDPS 499
           + +PS  +   CC + + S
Sbjct: 498 NALPSDEVMAKCCLEMEES 515

[Close]

BLAST of Sed0019920 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 455.3 bits (1170), Expect = 6.4e-128
Identity = 232/503 (46.12%), Postives = 334/503 (66.40%), Query Frame = 0


Query: 12  KDKQTLQHMEDITSNADEIQRQILVEILSTNGHVEYLQQHGLNGSTDASTFKNLIPVVSY 71
           KD + L+ +E++T N D +Q++++ EILS N   EYL++ GL G TD  TFK  +PVV Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 72  EQLKPYIDRIAYGDDSPILCAQPITQFFISSGTSGGEQKLIPMFEQEVER---IFSPIMK 131
           + LKP I RIA GD S IL + PIT+F  SSGTS GE+KL+P  +++++R   ++S +M 
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLM- 137

Query: 132 YEIPKMKQLFPNSHKGKEMFFLFNRPQARTKSGIIAQSVSTSFCKKPHLFN--NILTRGC 191
              P M    P   KGK ++FLF + +++T  G+ A+ V TS+ K        N      
Sbjct: 138 ---PVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVY 197

Query: 192 TSPNGVLLCTDTYQSTYSQLLCGLYQNESVFILGSLFISTLAYVLKFLENHWILLANDIR 251
           TSPN  +LC D+ QS Y+Q+LCGL     V  LG++F S L   + FL+ +W  LA+DI 
Sbjct: 198 TSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDIS 257

Query: 252 TGIINSKITDSLVRESVMNIL-EPNPHLADFIETECSK-GTWEGIITRLWPNAKYIKAIA 311
           TG ++S+I+D  ++ES+  IL +P+  LADFI + C +  +WEGIIT++WPN KY+  I 
Sbjct: 258 TGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIV 317

Query: 312 TGSMSQYILQLNYYSNNLPLC---------FLGLNLDPLCKPEEISYTLIPTMAYFEFLP 371
           TG+M+QYI  L YYS  LP+          + G+NL P+CKP E+SYT++P MAYFEFLP
Sbjct: 318 TGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP 377

Query: 372 IEREKPNGESRQDLVDLVDVKLGQEYELVVTTYAGLYRYCMGDILRVTGFKNKAPNFSFV 431
              E P  +S  +LV+L DV++G+EYELV+TTYAGL RY +GDIL+VTGF N AP F FV
Sbjct: 378 -HHEVPTEKS--ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFV 437

Query: 432 CRRNIILSLDLEKTEEGELHNAIQEGARLLGPFGAVVADYTSYADNRTIPGHYVLYWEIR 491
            R+N++LS++ +KT+E EL +A++  + LLG  G  V +YTSYA+ +TIPGHYV+YWE+ 
Sbjct: 438 RRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELL 497

Query: 492 IKEESSIIPSSIYEDCCPKWDPS 499
           +K++++     +   CC + + S
Sbjct: 498 VKDQTNPPNDEVMARCCLEMEES 513

The following BLAST results are available for this feature:

BLAST of Sed0019920 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140778.1	1.4e-204	70.99	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
KAG6570863.1	8.0e-197	68.39	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022944596.1	1.3e-194	67.99	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_022140368.1	3.3e-190	66.53	indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia]	[more]
XP_038902067.1	4.0e-188	66.01	indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida]	[more]

BLAST of Sed0019920 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81829	1.7e-157	54.33	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	2.2e-157	53.92	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	2.8e-136	49.69	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	4.3e-129	47.51	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
O82333	7.4e-129	47.48	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Sed0019920 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIS9	6.6e-205	70.99	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1FUU5	6.2e-195	67.99	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1CHU9	1.6e-190	66.53	indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ...	[more]
A0A5A7V3L5	1.0e-181	63.58	Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BJT8	1.0e-181	63.58	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Sed0019920 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27260.1	1.2e-158	54.33	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	1.6e-158	53.92	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	5.3e-130	47.48	Auxin-responsive GH3 family protein	[more]
AT1G59500.1	7.6e-129	45.29	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	6.4e-128	46.12	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 20..496 e-value: 1.1E-149 score: 499.8
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 9..493
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 9..493

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0019920.1	Sed0019920.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity