Sed0025323 (gene) Chayote v1

BLAST of Sed0025323 vs. NCBI nr
Match: XP_022959608.1 (uncharacterized protein LOC111460633 [Cucurbita moschata])

HSP 1 Score: 1554.7 bits (4024), Expect = 0.0e+00
Identity = 785/1000 (78.50%), Postives = 875/1000 (87.50%), Query Frame = 0

Query: 1   MAEYFRVNPAEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLC 60
           MA+YFR +P EFSL STV+LQKAWYL TVLLDIG PASVEE+ VRC LFSVTPDFVRYLC
Sbjct: 1   MADYFRFDPDEFSLQSTVELQKAWYLFTVLLDIGHPASVEEIVVRCELFSVTPDFVRYLC 60

Query: 61  QIPDSPISLVDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYF 120
           +IP+SPISL DN+LV IS+VA+S+VGRYFSK TNG D  RR+FGVLE NR  GS+VKTYF
Sbjct: 61  EIPNSPISLADNSLVFISVVAISSVGRYFSKATNGWDSLRRHFGVLEPNRLWGSDVKTYF 120

Query: 121 RKRRRSMVDSGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTL 180
           RKR+RS++DSGVPF AKRMLTSTS  GNGS LSMT+RIS N  EVPAD +TL S NSLT+
Sbjct: 121 RKRKRSILDSGVPFPAKRMLTSTSGIGNGSCLSMTRRISQNFDEVPADYLTLTSSNSLTV 180

Query: 181 DLPFENPKMGHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKD------ 240
           +LPFE+ +MGHLDV  D  PF L YADFP+ LM  DG+MG +N  Q+SKI+IKD      
Sbjct: 181 NLPFEHLEMGHLDVKIDEEPFPLDYADFPNFLMSHDGRMGIDNDAQASKILIKDDAGLLS 240

Query: 241 ----DCHIHEVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYK 300
               DCHI+EVQKDS   N+ H D+GM CNLDLSLGPPCSS+NTL C KILD NNKLA +
Sbjct: 241 ENGHDCHINEVQKDSSIPNVRHADRGMLCNLDLSLGPPCSSTNTLPCDKILDNNNKLAIR 300

Query: 301 SGNTSVLCLGNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTK 360
           SGNTSV CLG DM P VQP EV+N + +++LSNI EWKLEDEKNKICE++N S++T+TT+
Sbjct: 301 SGNTSVSCLGGDMQPRVQPSEVENGICTKHLSNISEWKLEDEKNKICEMKNCSENTKTTE 360

Query: 361 NHLVHLEGHEITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVK 420
           NHLVHLEGH +TGEA ++FL LRNPV IS MD DDI RKG T+I CSLPDHL +Q+PSVK
Sbjct: 361 NHLVHLEGHAVTGEAGRNFLDLRNPVTISNMDRDDISRKGNTEIPCSLPDHLFDQQPSVK 420

Query: 421 TFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQ 480
           TF  LDGSQKC L  E+V MESAV+KEVSQ +EQQ+R N+  K  SK+QKFKK+SNENV 
Sbjct: 421 TFGELDGSQKCTLVPEKVLMESAVSKEVSQTLEQQNRYNSVHKPTSKVQKFKKSSNENVH 480

Query: 481 IKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVN 540
           IKENPLD+ TSS+KL++TSFPQFESF+IEEEEGSGGYGTVYRARRKND K IAIKCPHVN
Sbjct: 481 IKENPLDSPTSSLKLEKTSFPQFESFIIEEEEGSGGYGTVYRARRKNDGKRIAIKCPHVN 540

Query: 541 AHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRL 600
           AHK+++NNELKMLERFGGRNFIIK EG+FSS  + ECL+LEHVEHDRPEVLKKEIDIVRL
Sbjct: 541 AHKHNVNNELKMLERFGGRNFIIKCEGSFSSG-NSECLMLEHVEHDRPEVLKKEIDIVRL 600

Query: 601 QWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSN 660
           QWYGYCLFKAL  LHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+DLQ+KYSIASKSN
Sbjct: 601 QWYGYCLFKALACLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDLQQKYSIASKSN 660

Query: 661 TSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLK 720
           TS+HASSS +  PHS+SGS VKD+  FRGFA L+KGE GKSKQ  EHD+ LKKRA++PLK
Sbjct: 661 TSHHASSSHVTAPHSLSGSSVKDQN-FRGFAYLSKGETGKSKQACEHDQTLKKRAYIPLK 720

Query: 721 KYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRN 780
           KYPDMGGGSAVRSQGADGS ITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNA RN
Sbjct: 721 KYPDMGGGSAVRSQGADGSGITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNALRN 780

Query: 781 ADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEG 840
           +DH  QNSSDLRRKRIAAPPGKED+KIIHPSPIL+HCSGI VAGSR+ KAKGDA RKKEG
Sbjct: 781 SDHVTQNSSDLRRKRIAAPPGKEDNKIIHPSPILVHCSGISVAGSRVLKAKGDAKRKKEG 840

Query: 841 SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGS 900
           SCAGTKGFRAPEVLFRSLHQGPQVD+WSAGVTLLYLM+GRSPFTGDPEQN+KDIAKLRGS
Sbjct: 841 SCAGTKGFRAPEVLFRSLHQGPQVDIWSAGVTLLYLMVGRSPFTGDPEQNLKDIAKLRGS 900

Query: 901 EDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCL 960
           EDLWEVAKLHDRESAFP ELF+ITSFPA DLQSW+ +HTKRPDFLKLIP SLF+LVDKCL
Sbjct: 901 EDLWEVAKLHDRESAFPRELFDITSFPARDLQSWVKAHTKRPDFLKLIPTSLFNLVDKCL 960

Query: 961 TVNPRQRISAEEALKHEFFNSCHERLRKQRLLRRGSSSNS 990
           TVNPRQRI+AEEALKHEFFNSCH+RLRKQR+L RGS  NS
Sbjct: 961 TVNPRQRITAEEALKHEFFNSCHDRLRKQRMLLRGSRFNS 998

BLAST of Sed0025323 vs. NCBI nr
Match: KAG7025348.1 (Cell division cycle 7-related protein kinase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1553.9 bits (4022), Expect = 0.0e+00
Identity = 784/1000 (78.40%), Postives = 873/1000 (87.30%), Query Frame = 0

Query: 1   MAEYFRVNPAEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLC 60
           MA+YFR +P EFSL STV+LQKAWYL TVLLDIG PASVEE+ VRC LFSVTPDFVRYLC
Sbjct: 1   MADYFRFDPDEFSLQSTVELQKAWYLFTVLLDIGHPASVEEIVVRCELFSVTPDFVRYLC 60

Query: 61  QIPDSPISLVDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYF 120
           +IP+SPISL DN+LV IS+VA+S+VGRYFSK TNG D  RR+FGVLE NR  GS+VKTYF
Sbjct: 61  EIPNSPISLADNSLVFISVVAISSVGRYFSKATNGWDSLRRHFGVLEPNRLWGSDVKTYF 120

Query: 121 RKRRRSMVDSGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTL 180
           RKR+RS++DSGVPF AKRMLTSTS  GNGS LSMT+RIS N  EVPAD +TL S NSLT+
Sbjct: 121 RKRKRSILDSGVPFPAKRMLTSTSGIGNGSCLSMTRRISQNFDEVPADYLTLTSSNSLTV 180

Query: 181 DLPFENPKMGHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKD------ 240
           +LPFE+ +MGHLDV  D  PF L YADFP+ LM  DG+MG +N  Q+SKI+IKD      
Sbjct: 181 NLPFEHLEMGHLDVKIDEEPFPLDYADFPNFLMSHDGRMGIDNDAQASKILIKDDAGLLS 240

Query: 241 ----DCHIHEVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYK 300
               DCHI+EVQKDS   N+ H D+GM CNLDLSLGPPCSS+NTL C KILD NNKLAY+
Sbjct: 241 ENGHDCHINEVQKDSSIPNVRHADRGMLCNLDLSLGPPCSSTNTLPCDKILDNNNKLAYR 300

Query: 301 SGNTSVLCLGNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTK 360
           SGNTSV CLG DM P VQP EV+N + + +LSNI EWKLEDEKNKICE++N S++T+TT+
Sbjct: 301 SGNTSVSCLGGDMQPRVQPSEVENGICTMHLSNISEWKLEDEKNKICEMKNCSENTKTTE 360

Query: 361 NHLVHLEGHEITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVK 420
           NHLVHLEGH +TGEA ++FL LRNPV IS MD DDI RKG TQI CSLPDHL +Q+PSVK
Sbjct: 361 NHLVHLEGHAVTGEAGRNFLDLRNPVTISNMDRDDISRKGNTQIPCSLPDHLFDQQPSVK 420

Query: 421 TFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQ 480
           TF  LDGSQKC L  E+V MESAV+KEVSQP+EQQ+R N+  K  SK+QKFKK+SNENV 
Sbjct: 421 TFGELDGSQKCTLVPEKVLMESAVSKEVSQPLEQQNRYNSVHKPTSKVQKFKKSSNENVH 480

Query: 481 IKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVN 540
           IKENPLD+ TSS+KL++TS PQFESF+IEEEEGSGGYGTVYRARRKND K IAIKCPHVN
Sbjct: 481 IKENPLDSPTSSLKLEKTSLPQFESFIIEEEEGSGGYGTVYRARRKNDGKRIAIKCPHVN 540

Query: 541 AHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRL 600
           AHK+++NNELKMLERFGGRNFIIK EG+FSS  + ECL+LEHVEHDRPEVLKKEIDIVRL
Sbjct: 541 AHKHNVNNELKMLERFGGRNFIIKCEGSFSSG-NSECLMLEHVEHDRPEVLKKEIDIVRL 600

Query: 601 QWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSN 660
           QWYGYCLFKAL  LHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+DLQ+KYSIASKSN
Sbjct: 601 QWYGYCLFKALACLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDLQQKYSIASKSN 660

Query: 661 TSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLK 720
           TS+HASSS +  PHS+SGS VKD+  FRGFA L+KGE GK+KQ  EHD+ LKKRA++PLK
Sbjct: 661 TSHHASSSHVTAPHSLSGSSVKDQN-FRGFAYLSKGETGKAKQACEHDQTLKKRAYIPLK 720

Query: 721 KYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRN 780
           KYPDMGGGSAVRSQGADGS ITSAKDVTSVRTLS ENMREPLPCQGRKELLSLVQNA RN
Sbjct: 721 KYPDMGGGSAVRSQGADGSGITSAKDVTSVRTLSAENMREPLPCQGRKELLSLVQNALRN 780

Query: 781 ADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEG 840
           +DH  QNSSDLRRKRIAAPPGKED+KIIHPSPIL+HCSGI VAGSR+ KAKGDA RKKEG
Sbjct: 781 SDHVTQNSSDLRRKRIAAPPGKEDNKIIHPSPILVHCSGISVAGSRVLKAKGDAKRKKEG 840

Query: 841 SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGS 900
           SCAGTKGFRAPEVLFRSLHQGPQVD+WSAGVTLLYLM+GRSPFTGDPEQN+KDIAKLRGS
Sbjct: 841 SCAGTKGFRAPEVLFRSLHQGPQVDIWSAGVTLLYLMVGRSPFTGDPEQNLKDIAKLRGS 900

Query: 901 EDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCL 960
           EDLWEVAKLHDRESAFP ELF+ITSFPA DLQSW+ +HTKRPDF KLIP SLF+LVDKCL
Sbjct: 901 EDLWEVAKLHDRESAFPRELFDITSFPARDLQSWVKAHTKRPDFFKLIPTSLFNLVDKCL 960

Query: 961 TVNPRQRISAEEALKHEFFNSCHERLRKQRLLRRGSSSNS 990
           TVNPRQRI+AEEALKHEFFNSCH+RLRKQR+L RGS  NS
Sbjct: 961 TVNPRQRITAEEALKHEFFNSCHDRLRKQRMLLRGSRFNS 998

BLAST of Sed0025323 vs. NCBI nr
Match: XP_023004763.1 (uncharacterized protein LOC111497968 [Cucurbita maxima])

HSP 1 Score: 1540.0 bits (3986), Expect = 0.0e+00
Identity = 777/1000 (77.70%), Postives = 873/1000 (87.30%), Query Frame = 0

Query: 1   MAEYFRVNPAEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLC 60
           MA+YFR  P EFSLHSTV+LQKAWYL TVLLDIG PASVEE+ VRC LFSVTPDFVRYLC
Sbjct: 1   MADYFRFYPDEFSLHSTVELQKAWYLFTVLLDIGHPASVEEIVVRCELFSVTPDFVRYLC 60

Query: 61  QIPDSPISLVDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYF 120
           +IP+SPISL D++LV IS+VA+S+VGRYFSK TNG D  RR+F VLE NR  GS+VKTYF
Sbjct: 61  KIPNSPISLADSSLVFISVVAISSVGRYFSKATNGWDSLRRHFEVLEPNRLWGSDVKTYF 120

Query: 121 RKRRRSMVDSGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTL 180
           RKR+RS++DSGVPF AKRMLTSTS  GNGS LSMT+RISHN  EVPAD +TL S NSLT+
Sbjct: 121 RKRKRSILDSGVPFPAKRMLTSTSGIGNGSCLSMTRRISHNFDEVPADYLTLTSSNSLTV 180

Query: 181 DLPFENPKMGHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKD------ 240
           +LPFE+ + GHLDV  D  PF L YADFP+ LM  DG+MG +NAVQ+SKI+IKD      
Sbjct: 181 NLPFEHLETGHLDVKIDEEPFPLDYADFPNFLMSHDGRMGIDNAVQASKILIKDDAGFFS 240

Query: 241 ----DCHIHEVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYK 300
               DCHI+EVQK S   N+ H D+GM CNLDL+LGPPCSS+NTLSC KIL+ +NKLAY+
Sbjct: 241 ENGHDCHINEVQKVSSIPNVRHADRGMLCNLDLALGPPCSSTNTLSCDKILENHNKLAYR 300

Query: 301 SGNTSVLCLGNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTK 360
            GNTSV CLG DM P V+P EV+N + + +LSNI EWKLEDEKNKICE++N S++TRTT+
Sbjct: 301 CGNTSVSCLGGDMQPRVRPSEVENGICTMHLSNISEWKLEDEKNKICEMKNCSENTRTTE 360

Query: 361 NHLVHLEGHEITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVK 420
           NHLVHLEGHE+TGEA ++FL LRNPV  S MD DDI RKG+TQI CSLPDHL +Q+PSVK
Sbjct: 361 NHLVHLEGHEVTGEAGRNFLDLRNPVTFSNMDRDDISRKGKTQIPCSLPDHLFDQQPSVK 420

Query: 421 TFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQ 480
           TF  LDGSQKC L  +++ MESAV+KEVSQP+E Q++ N+  K  SK+QKFKKNSNENV 
Sbjct: 421 TFGELDGSQKCTLVPKKILMESAVSKEVSQPLEPQNQYNSVHKPTSKVQKFKKNSNENVH 480

Query: 481 IKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVN 540
           IKENPLD+ TSS+KL++TSFPQFESF+IEEEEGSGGYGTVYRARRKND K IAIKCPHVN
Sbjct: 481 IKENPLDSPTSSLKLEKTSFPQFESFIIEEEEGSGGYGTVYRARRKNDGKRIAIKCPHVN 540

Query: 541 AHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRL 600
           AHK+++NNELKMLERFGGRNFIIK EG+FSS  + ECL+LEHVEHDRPEVLKKEIDIVRL
Sbjct: 541 AHKHNVNNELKMLERFGGRNFIIKCEGSFSSG-NSECLMLEHVEHDRPEVLKKEIDIVRL 600

Query: 601 QWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSN 660
           QWYGYCLFKAL  LHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+DL +KYSIASKSN
Sbjct: 601 QWYGYCLFKALACLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDLHQKYSIASKSN 660

Query: 661 TSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLK 720
           TS+HASSS + VPHS+SGS VKD+  FRGFA L+KGE G SKQ  EHDK LKKRA++PLK
Sbjct: 661 TSHHASSSHVTVPHSLSGSSVKDQN-FRGFAYLSKGETGISKQACEHDKTLKKRAYIPLK 720

Query: 721 KYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRN 780
           KYPDMGGGSAVRSQGADGS ITSAKDVTSVRTLSTENMREPLPCQGRKELLSLV+NA RN
Sbjct: 721 KYPDMGGGSAVRSQGADGSGITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVKNALRN 780

Query: 781 ADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEG 840
           +DH  QNSSDLRRKRIAAPPGKED+KIIHPSPIL+HCSGI VAGSR+ KAKGDA RKKEG
Sbjct: 781 SDHVTQNSSDLRRKRIAAPPGKEDNKIIHPSPILVHCSGISVAGSRVLKAKGDAKRKKEG 840

Query: 841 SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGS 900
           SCAGTKGFRAPEVLFRSLHQGPQVD+WSAGVTLLYLM+GRSPF GDPEQN+KDIAKLRGS
Sbjct: 841 SCAGTKGFRAPEVLFRSLHQGPQVDIWSAGVTLLYLMVGRSPFIGDPEQNLKDIAKLRGS 900

Query: 901 EDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCL 960
           EDLWEVAKLHDRESAFP ELF+ITSFPA DLQSW+ +HTKRPDF KLIP SLF+LVDKCL
Sbjct: 901 EDLWEVAKLHDRESAFPRELFDITSFPARDLQSWVKAHTKRPDFFKLIPTSLFNLVDKCL 960

Query: 961 TVNPRQRISAEEALKHEFFNSCHERLRKQRLLRRGSSSNS 990
           TVNPRQRI+AEEALKHEFF+SCH+RLRKQR+L +GS  NS
Sbjct: 961 TVNPRQRITAEEALKHEFFSSCHDRLRKQRMLLQGSRFNS 998

BLAST of Sed0025323 vs. NCBI nr
Match: XP_023515313.1 (serine/threonine-protein kinase RIM15-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1536.2 bits (3976), Expect = 0.0e+00
Identity = 778/1000 (77.80%), Postives = 869/1000 (86.90%), Query Frame = 0

Query: 1   MAEYFRVNPAEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLC 60
           MA+YFR +P EFSLHSTV+LQKAWYL TVLLDIG PASVEE+ VRC LFSVTP+FVRYLC
Sbjct: 1   MADYFRFDPDEFSLHSTVELQKAWYLFTVLLDIGHPASVEEIVVRCELFSVTPEFVRYLC 60

Query: 61  QIPDSPISLVDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYF 120
           +IP+SPISL DN+LV IS+VA+S+VGRYFSK TNG D  RR+FGVLE NR   S+VKTYF
Sbjct: 61  KIPNSPISLADNSLVFISVVAISSVGRYFSKATNGWDSLRRHFGVLEPNRLWSSDVKTYF 120

Query: 121 RKRRRSMVDSGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTL 180
           RKR+RS++DSGVPF AKRMLTSTS  GNGS LSMT+RIS N  EVPAD +TL S NSLT+
Sbjct: 121 RKRKRSILDSGVPFPAKRMLTSTSGIGNGSCLSMTRRISQNFDEVPADYLTLTSSNSLTV 180

Query: 181 DLPFENPKMGHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKD------ 240
           +LPFE+ +MGHLDV  D  PF L YADFP+ LM  DG+MG +N VQ+SKI+IKD      
Sbjct: 181 NLPFEHLEMGHLDVKIDEEPFPLDYADFPNFLMSHDGRMGIDNDVQASKILIKDDAGFLS 240

Query: 241 ----DCHIHEVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYK 300
               DCHI+EV+KDS   N+ H D+GM CNLDLSLGPPCSS+NTL C KIL+ NNKLAY+
Sbjct: 241 ENGHDCHINEVRKDSSIPNVRHADRGMLCNLDLSLGPPCSSTNTLPCDKILENNNKLAYR 300

Query: 301 SGNTSVLCLGNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTK 360
           SGNT V CLG DM P VQP EV+N +++ +LSNI EWKLEDEKNKICE++N S++TRTT+
Sbjct: 301 SGNTPVSCLGGDMQPRVQPSEVENGIYTMHLSNITEWKLEDEKNKICEMKNCSENTRTTE 360

Query: 361 NHLVHLEGHEITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVK 420
           NHLVHLEGH +TGEA ++FL LRNPV IS MD DDI RKG TQI CSLPDHL +Q+PSVK
Sbjct: 361 NHLVHLEGHAVTGEAGRNFLDLRNPVTISNMDKDDISRKGNTQIPCSLPDHLFDQQPSVK 420

Query: 421 TFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQ 480
           TF  LDGSQKC L  E+V MESAV+KEVSQP+EQQ+R N+  K  SK+QKFKKNSNENV 
Sbjct: 421 TFGELDGSQKCTLVPEKVLMESAVSKEVSQPLEQQNRYNSVHKPTSKVQKFKKNSNENVH 480

Query: 481 IKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVN 540
           IKENPLD+ TSS+KL++TSFPQ ESF+IEEEEGSGGYGTVYRARRKND K IAIKCPHVN
Sbjct: 481 IKENPLDSPTSSLKLEKTSFPQLESFIIEEEEGSGGYGTVYRARRKNDGKRIAIKCPHVN 540

Query: 541 AHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRL 600
           AHK+++NNELKMLERFGGRNFIIK EG+FSS  + ECL+LEHVEHDRPEVLKK+IDIVRL
Sbjct: 541 AHKHNVNNELKMLERFGGRNFIIKCEGSFSSG-NSECLMLEHVEHDRPEVLKKDIDIVRL 600

Query: 601 QWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSN 660
           QWYGYCLFKAL  LHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+DLQ+KYSIASKSN
Sbjct: 601 QWYGYCLFKALACLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDLQQKYSIASKSN 660

Query: 661 TSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLK 720
           TS+HASSS + VPHS+SGS VKD+  FRGFA L+KGE GKSKQ  EHDK LKKRA++PLK
Sbjct: 661 TSHHASSSHVAVPHSLSGSSVKDQN-FRGFAYLSKGETGKSKQACEHDKTLKKRAYIPLK 720

Query: 721 KYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRN 780
           KYPDMGGGSAVRSQGADGS ITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNA RN
Sbjct: 721 KYPDMGGGSAVRSQGADGSGITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNALRN 780

Query: 781 ADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEG 840
           +DH  QNSSDLRRKRIAAPP KED+KIIHPSPIL+HCSGI VAGSR+ KAKGDA RKKEG
Sbjct: 781 SDHVTQNSSDLRRKRIAAPPRKEDNKIIHPSPILVHCSGISVAGSRVLKAKGDAKRKKEG 840

Query: 841 SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGS 900
           SCAGTKGFRAPEVLFRSLHQGPQVD+WSAGVTLLYLM+G  PF G   +N+KDIAKLRGS
Sbjct: 841 SCAGTKGFRAPEVLFRSLHQGPQVDIWSAGVTLLYLMVGXKPFHG---RNLKDIAKLRGS 900

Query: 901 EDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCL 960
           EDLWEVAKLHDRESAFP ELF+ITSFPA DLQSW+ +HTKRPDF KLIP SLF+LVDKCL
Sbjct: 901 EDLWEVAKLHDRESAFPRELFDITSFPARDLQSWVKAHTKRPDFFKLIPTSLFNLVDKCL 960

Query: 961 TVNPRQRISAEEALKHEFFNSCHERLRKQRLLRRGSSSNS 990
           TVNPRQRI+AEEALKHEFFNSCH+RLRKQR+L RGS  NS
Sbjct: 961 TVNPRQRITAEEALKHEFFNSCHDRLRKQRMLLRGSRFNS 995

BLAST of Sed0025323 vs. NCBI nr
Match: XP_038876183.1 (uncharacterized protein LOC120068469 [Benincasa hispida])

HSP 1 Score: 1477.6 bits (3824), Expect = 0.0e+00
Identity = 765/1019 (75.07%), Postives = 849/1019 (83.32%), Query Frame = 0

Query: 1    MAEYFRVNPAEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLC 60
            MA+YFR N AE+SLHSTV+LQK+WYL T+LLDIGRPASVEELAVRC LFSVTP  VRYLC
Sbjct: 1    MADYFRFNSAEYSLHSTVELQKSWYLFTILLDIGRPASVEELAVRCELFSVTPYIVRYLC 60

Query: 61   QIPDSPISLVDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYF 120
            + P SPI L D+ LV IS+VA+SAVGRYF+K T+G DF RR FGV E +RFCG ++KTY 
Sbjct: 61   ENPGSPICLGDDGLVYISIVAISAVGRYFTKTTSGWDFLRRGFGVTEPDRFCGRDIKTYC 120

Query: 121  RKRRRSMVDSGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTL 180
            RKRRRS++DSG  F  KRMLTSTS  GN S LS+T+RISHN AEVPAD VTL SLNS T+
Sbjct: 121  RKRRRSVLDSGGRFDGKRMLTSTSGIGNESCLSITRRISHNYAEVPADYVTLTSLNSFTV 180

Query: 181  DLPFENPKMGHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKD------ 240
            DL FE   MGHLDV FD MP SL Y D P  LM   GQMGA+ +VQ+SK MIKD      
Sbjct: 181  DLLFEKLSMGHLDVKFDEMPCSLDYPDSPYFLMSHAGQMGADISVQASKTMIKDDAGPLS 240

Query: 241  ----DCHIHEVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYK 300
                DCH+HEVQKDS   NL HTD+ MSCN D SLG  CSS+NTL    + DKN KLAYK
Sbjct: 241  ENGHDCHMHEVQKDSSMPNLRHTDREMSCNFDFSLGASCSSTNTL----VYDKNCKLAYK 300

Query: 301  SGNTSVLCLGNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTK 360
             GN S LC G+DM P VQP EVDN + +R+L NILEWKLEDEKNKICE QN S+ TRTT+
Sbjct: 301  LGNISALCHGDDMQPYVQPLEVDNGICTRHLPNILEWKLEDEKNKICETQNCSEYTRTTE 360

Query: 361  NHLVHLEGHEITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVK 420
            N   HLEGH + GEA ++FL LRNPV  + MDG+DI RKGETQI CS+PD   N+EPSVK
Sbjct: 361  NR-AHLEGHIVIGEAERNFLDLRNPVT-TYMDGEDISRKGETQIPCSIPDQPCNEEPSVK 420

Query: 421  TFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQ 480
            T   +DGSQKC  S EEV MESAV KEVS PV+QQ+  NN  KLMSK++K KKNSN NV 
Sbjct: 421  TLGEVDGSQKCTPSPEEVLMESAVYKEVSHPVKQQNCYNNVNKLMSKVKKVKKNSNGNVH 480

Query: 481  IKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVN 540
            IKENPLD T+ SMKL++TSFPQFESF+IEEEEGSGGYGTVYRARRKND K IAIKCPHVN
Sbjct: 481  IKENPLDPTSPSMKLEKTSFPQFESFIIEEEEGSGGYGTVYRARRKNDGKRIAIKCPHVN 540

Query: 541  AHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRL 600
            AHK+++NNELKMLERFGGRNFIIKYEG+ SS  + ECLILEHVEHDRPEVLKKEIDIVRL
Sbjct: 541  AHKHNVNNELKMLERFGGRNFIIKYEGSISSG-NSECLILEHVEHDRPEVLKKEIDIVRL 600

Query: 601  QWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSN 660
            QWYG+CLF+AL  LHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+DLQ+KYSIASKSN
Sbjct: 601  QWYGFCLFRALACLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDLQQKYSIASKSN 660

Query: 661  TSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLK 720
            +S+HA    +PVPHSVSGS VK+KK  RGF S+++GE GKSKQ  E  KNLKKR +VPLK
Sbjct: 661  SSHHAFLDHVPVPHSVSGSAVKEKKNLRGFVSVSRGETGKSKQPGELKKNLKKRVYVPLK 720

Query: 721  KYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRN 780
            KYPDM GGSAVRSQGADGS ITSAKDVTS RT STENMREPLPCQGRKELLSLVQNA RN
Sbjct: 721  KYPDMHGGSAVRSQGADGSGITSAKDVTSARTFSTENMREPLPCQGRKELLSLVQNALRN 780

Query: 781  ADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEG 840
            ADHA QNSSDLRRKRIAAPP KED+ IIHPSP+L+HC+GI +AGSR+ + KGDA RK+EG
Sbjct: 781  ADHAPQNSSDLRRKRIAAPPDKEDNNIIHPSPMLVHCTGISLAGSRVLRTKGDARRKREG 840

Query: 841  SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGS 900
            SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLM+G+SPFTGDPEQNIKDIAKLRGS
Sbjct: 841  SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMVGKSPFTGDPEQNIKDIAKLRGS 900

Query: 901  EDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCL 960
            EDLWEVAKLHDRESAFPGELFNI SFP MDLQSW+ +HTKRPDF+KLIPRSLFDLVDKCL
Sbjct: 901  EDLWEVAKLHDRESAFPGELFNIKSFPPMDLQSWVKAHTKRPDFVKLIPRSLFDLVDKCL 960

Query: 961  TVNPRQRISAEEALKHEFFNSCHERLRKQRLLRRGSSSN---------SVQGQSFEILR 1000
            TVNPRQRI+AEEALKHEFFN C+ERLRKQR+LRRGSSSN         +V GQS ++ R
Sbjct: 961  TVNPRQRITAEEALKHEFFNPCYERLRKQRMLRRGSSSNTTDVLSECEAVLGQSIKLSR 1012

BLAST of Sed0025323 vs. ExPASy Swiss-Prot
Match: Q54DK3 (Probable serine/threonine-protein kinase cdc7 OS=Dictyostelium discoideum OX=44689 GN=cdc7 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 5.5e-27
Identity = 135/572 (23.60%), Postives = 242/572 (42.31%), Query Frame = 0

Query: 402  NNQEPSVKTFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFK 461
            +NQ+ +V    N + +   N +       +  N   +   +QQ + +ND KL+ K     
Sbjct: 536  SNQKLTVNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTIQQQQQQQSNDIKLLLK-NSIY 595

Query: 462  KNSNENVQIKENPLDTTTSSMKLKETSFPQF-ESFVIEEEEGSGGYGTVYRA---RRKND 521
             + N+  ++   P + T       ++ +P+    + I E+ G G +  VY++      N 
Sbjct: 596  SSRNDLKELLSQPTEYT-------DSEYPEIVGKYRILEKIGQGTFSGVYKSVCIDGPNI 655

Query: 522  DKHIAIKCPHVNAHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRP 581
               +A+K     +    I NE+  L R GG   +    GA    + +  LIL   EHD  
Sbjct: 656  GLIVALKRVAPTSSPARILNEIHSLLRVGGHYNVSALFGALRYKD-QVTLILPFFEHDSF 715

Query: 582  EVLKKEIDIVRLQWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVD 641
            +    ++    ++ Y Y LF +L ++H+  + HRDVKP NFL+S K N   LIDF LA +
Sbjct: 716  KDYFFQMSNENIKHYLYALFDSLRHIHQNNICHRDVKPTNFLYSIKNNSFLLIDFGLAQE 775

Query: 642  LQKKYSIASKSNTSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHD 701
            +       S SN++ +++S+     +S S S   +        + N      S   F + 
Sbjct: 776  MPN-----SNSNSNSNSNSNSNSNSNSNSNSNSNNNNNNNNNNTNNNFNGNNSNNDFNNF 835

Query: 702  KNLKKRAFVPLKKYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRK 761
             N+                              +++ +  +  + +  N           
Sbjct: 836  INMNN----------------------------SNSNNNNNNNSNNNNNNNNN------- 895

Query: 762  ELLSLVQNARRNADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIP 821
                   N   N +    N+S+  ++ I  P   E+      +    + +    + S   
Sbjct: 896  ------NNNNNNNNSNNNNNSNNSQEEIILPSTNENGTTTSNASSTSNTTSSSSSSSNKS 955

Query: 822  KAKGDAMRKKEGSCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPE 881
            K   +  + +    AGT+GFRAPEVL +   Q   +D+WS GV LL ++ GR PF   P+
Sbjct: 956  KNLRNDPKPQPAPRAGTRGFRAPEVLLKYNKQTTAIDIWSVGVILLCMISGRYPFFISPD 1015

Query: 882  Q--NIKDIAKLRGSEDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLK 941
               ++ +I  + G++ + ++A L +++ +      +I   P  DL   + S +      +
Sbjct: 1016 DMTSLAEIVSIIGTKKIVDIAHLLEKKISIS---HSIPPTPWRDLSRRLRSESSCDK--Q 1047

Query: 942  LIPRSLFDLVDKCLTVNPRQRISAEEALKHEF 968
             +P  L+DL+++CL  NP  RI+A EAL H F
Sbjct: 1076 DVPVELYDLLERCLDPNPLTRITASEALLHPF 1047

BLAST of Sed0025323 vs. ExPASy Swiss-Prot
Match: O00311 (Cell division cycle 7-related protein kinase OS=Homo sapiens OX=9606 GN=CDC7 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.8e-23
Identity = 148/625 (23.68%), Postives = 244/625 (39.04%), Query Frame = 0

Query: 429 MESAVNKEVSQPV---EQQDRSNNDPKLMSKIQKFKKNSNENVQIKENPLDTTTSSMKLK 488
           ME+++  ++ +P+    Q+DR   +  L    Q FK             L      ++  
Sbjct: 1   MEASLGIQMDEPMAFSPQRDRFQAEGSLKKNEQNFK-------------LAGVKKDIEKL 60

Query: 489 ETSFPQFES-FVIEEEEGSGGYGTVYRARRK---NDDKHIAIKCPHVNAHKNSINNELKM 548
             + PQ  + F IE++ G G + +VY A  +     ++ IA+K     +H   I  EL+ 
Sbjct: 61  YEAVPQLSNVFKIEDKIGEGTFSSVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQC 120

Query: 549 LERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFKALT 608
           L   GG++ ++  +  F  ++H   + + ++EH+    +   +    ++ Y   LFKAL 
Sbjct: 121 LTVAGGQDNVMGVKYCFRKNDH-VVIAMPYLEHESFLDILNSLSFQEVREYMLNLFKALK 180

Query: 609 YLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA-------VDLQKKYSIASK-----SN 668
            +H+ G++HRDVKP NFL++R+  K  L+DF LA       ++L K     ++      N
Sbjct: 181 RIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIELLKFVQSEAQQERCSQN 240

Query: 669 TSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLK 728
            S+  + ++IP    +SG V K              E  +   T    K     A + +K
Sbjct: 241 KSHIITGNKIP----LSGPVPK--------------ELDQQSTTKASVKRPYTNAQIQIK 300

Query: 729 KYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRK-ELLSLVQNARR 788
           +  D   GS   S       +   ++     ++S E+    L  Q +  ++LS     ++
Sbjct: 301 QGKDGKEGSVGLSVQRS---VFGERNFNIHSSISHESPAVKLMKQSKTVDVLSRKLATKK 360

Query: 789 NADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKE 848
            A      +S + RK  ++ P            +   C                + R++ 
Sbjct: 361 KAISTKVMNSAVMRKTASSCPASLTCDCYATDKVCSICL---------------SRRQQV 420

Query: 849 GSCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPF--TGDPEQNIKDIAKL 908
              AGT GFRAPEVL +  +Q   +D+WSAGV  L L+ GR PF    D    +  I  +
Sbjct: 421 APRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTI 480

Query: 909 RGSEDLWEVAKLHDRESAFPGELFNITSFPAMDLQ-----------------SWIMSHTK 968
           RGS +  + AK       F   +      PA DL+                 S I  H  
Sbjct: 481 RGSRETIQAAK------TFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQGHAS 540

BLAST of Sed0025323 vs. ExPASy Swiss-Prot
Match: Q9Z0H0 (Cell division cycle 7-related protein kinase OS=Mus musculus OX=10090 GN=Cdc7 PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 3.1e-22
Identity = 133/560 (23.75%), Postives = 216/560 (38.57%), Query Frame = 0

Query: 495 FVIEEEEGSGGYGTVYRARRKNDDKH---IAIKCPHVNAHKNSINNELKMLERFGGRNFI 554
           F I+++ G G + +VY A  +  + H   IA+K     +H   I  EL+ L   GG++ +
Sbjct: 52  FKIKDKIGEGTFSSVYLATAQLQEGHEEKIALKHLIPTSHPMRIAAELQCLTVAGGQDNV 111

Query: 555 IKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFKALTYLHKQGVMHR 614
           +  +  F  ++H   + + ++EH+    +   +    ++ Y Y LF AL  +H+ G++HR
Sbjct: 112 MGLKYCFRKNDH-VVIAMPYLEHESFLDILNSLSFQEVREYMYNLFVALKRIHQFGIVHR 171

Query: 615 DVKPGNFLFSRKQNKGYLIDFNLA-------VDLQKKYSIASK----SNTSYHASSSR-- 674
           DVKP NFL++R+  K  L+DF LA       ++L K     ++    S   YH       
Sbjct: 172 DVKPSNFLYNRRLKKYALVDFGLAQGTRDTKIELLKFVQSEAQQEDCSRNKYHGVVGHKG 231

Query: 675 ---IPVPHSVSGSVVKDKKIFRGFAS--LNKGEAGKSKQTFEHDKNLKKRAFVPLKKYPD 734
               P P +V         + R +    + +G+ GK +                      
Sbjct: 232 LLSRPAPKTVDQQCTPKTSVKRSYTQVHIKQGKDGKERSV-------------------- 291

Query: 735 MGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRK-ELLSLVQNARRNADH 794
             G S  RS       +   ++     ++S E+  E L  Q +  +++S     ++ A  
Sbjct: 292 --GLSVQRS-------VFGERNFNIHSSISHESPAEKLIKQSKTVDIISRKLATKKTAIS 351

Query: 795 AIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEGSCA 854
               +S +R             +     P ++ C      GS    +   + R++    A
Sbjct: 352 TKAMNSVMR-------------ETARSCPAVLTCD---CYGSDRVCSVCLSRRQQVAPRA 411

Query: 855 GTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPF--TGDPEQNIKDIAKLRGSE 914
           GT GFRAPEVL +   Q   +D+WSAGV  L L+ GR PF    D    +  I  +RGS 
Sbjct: 412 GTPGFRAPEVLTKCPDQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTIRGSR 471

Query: 915 DLWEVAKLHDRESAFPGELFNITSFPAMDLQS---------------------------- 969
           +  + AK      AF   +      PA DL++                            
Sbjct: 472 ETIQAAK------AFGKSVLCSKEVPAQDLRALCERLRGLDSTTPRSASGPPGNASYDPA 531

BLAST of Sed0025323 vs. ExPASy Swiss-Prot
Match: Q9UQY9 (Cell cycle protein kinase spo4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spo4 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 2.6e-16
Identity = 60/162 (37.04%), Postives = 84/162 (51.85%), Query Frame = 0

Query: 822 DAMRKKEGSCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPF--TGDPEQN 881
           D    K    AGT+GFRAPEVLFR  +Q   +DVWS GV LL  +  R PF    D    
Sbjct: 252 DPRPTKRADRAGTRGFRAPEVLFRCQNQTSSIDVWSVGVILLCFLTHRYPFFRCEDDIDA 311

Query: 882 IKDIAKLRGSEDLWEVAKLHDRESAFPGELFNITSFPAMDLQ-SW---IMSHTKRPDFLK 941
           I ++A + G   +   A LH       G++++      +D + +W   I S TK  + L 
Sbjct: 312 IVELAHIFGRNGMSNCALLH-------GQIWSDNIPTLLDQKHNWLDLIASITKNDENLI 371

Query: 942 LIPRS------LFDLVDKCLTVNPRQRISAEEALKHEFFNSC 972
           L   S        DL+DK L ++P +R+ A+ AL+HEFFN+C
Sbjct: 372 LETSSDYQVALAIDLLDKLLELHPSKRVKAKTALQHEFFNAC 406

HSP 2 Score: 50.1 bits (118), Expect = 1.7e-04
Identity = 41/197 (20.81%), Postives = 89/197 (45.18%), Query Frame = 0

Query: 441 VEQQDRSNNDPKLMSKIQKFKKNSNENVQIKENPLDTTTSSMKLKETSFPQFES--FVIE 500
           V+++DR    P++   +Q F     +   +K     + +S  K  +  F  +++  ++  
Sbjct: 18  VDREDR----PEIAKLVQAFPTIEEDYHVVKLVGAGSFSSVFKAVDRHFDSYDNSYWISS 77

Query: 501 EEEGSGGYGTVYRARRKNDDKHIAIKCPHVNAHKNSINNELKMLERFGGRNFIIKYEGAF 560
           + E    +    R +    +  +A+K  +     + I  EL+ML    G + ++    A 
Sbjct: 78  QIEDQMPHDKTKRPK----NHFVALKRIYATVLPSRIQTELEMLHELRGSDCVLNMITAV 137

Query: 561 SSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFKALTYLHKQGVMHRDVKPGNF 620
              + +  ++L  ++H        +  +  +  Y   L K L ++  +G++HRD+KPGNF
Sbjct: 138 RHQD-QVLIVLPFIQHAEFRDFYMKYSLPEIGAYLRDLLKGLAHIDAKGIIHRDIKPGNF 197

Query: 621 LFSRKQNKGYLIDFNLA 636
            ++    +G ++DF LA
Sbjct: 198 AWNPYTQRGVILDFGLA 205

BLAST of Sed0025323 vs. ExPASy Swiss-Prot
Match: P06243 (Cell division control protein 7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CDC7 PE=1 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 3.4e-16
Identity = 113/542 (20.85%), Postives = 199/542 (36.72%), Query Frame = 0

Query: 502 GSGGYGTVYRARR--------------KNDDKHIAIKCPHVNAHKNSINNELKMLERFGG 561
           G G + +VY+A+                    ++A+K  +V +    I NEL +L    G
Sbjct: 40  GEGTFSSVYKAKDITGKITKKFASHFWNYGSNYVALKKIYVTSSPQRIYNELNLLYIMTG 99

Query: 562 RNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFKALTYLHKQG 621
            + +     A      +   +L +  H+      +++ I  ++ Y + L +AL ++H +G
Sbjct: 100 SSRVAPLCDA-KRVRDQVIAVLPYYPHEEFRTFYRDLPIKGIKKYIWELLRALKFVHSKG 159

Query: 622 VMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSNTSYHASSSRIPVPHSVSG 681
           ++HRD+KP NFLF+ +  +G L+DF LA + Q  Y                         
Sbjct: 160 IIHRDIKPTNFLFNLELGRGVLVDFGLA-EAQMDYK------------------------ 219

Query: 682 SVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLKKYPDMGGGSAVRSQGADG 741
           S++  +  +  +A+ N            HD                  GG ++R      
Sbjct: 220 SMISSQNDYDNYANTN------------HD------------------GGYSMR------ 279

Query: 742 SVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRNADHAIQNSSDLRRKRIAA 801
                             N  +  PC  R +      N                      
Sbjct: 280 ------------------NHEQFCPCIMRNQYSPNSHN--------------------QT 339

Query: 802 PP--GKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEGSCAGTKGFRAPEVLFR 861
           PP    ++ K++H    L + +G+ +        K +  R K  + AGT+GFRAPEVL +
Sbjct: 340 PPMVTIQNGKVVH----LNNVNGVDLTKG---YPKNETRRIKRANRAGTRGFRAPEVLMK 399

Query: 862 SLHQGPQVDVWSAGVTLLYLMIGRSPF--TGDPEQNIKDIAKLRGSEDLWEVAKLH---- 921
              Q  ++D+WS GV LL L+  R P   + D   ++ ++  + G ++L + A LH    
Sbjct: 400 CGAQSTKIDIWSVGVILLSLLGRRFPMFQSLDDADSLLELCTIFGWKELRKCAALHGLGF 459

Query: 922 -------DRESAFPGEL-----------------------FNITSFPAMDLQSWIMSHTK 974
                  D+ + +   L                       F    F   +L   +    +
Sbjct: 460 EASGLIWDKPNGYSNGLKEFVYDLLNKECTIGTFPEYSVAFETFGFLQQELHDRMSIEPQ 474

BLAST of Sed0025323 vs. ExPASy TrEMBL
Match: A0A6J1H6F7 (uncharacterized protein LOC111460633 OS=Cucurbita moschata OX=3662 GN=LOC111460633 PE=4 SV=1)

HSP 1 Score: 1554.7 bits (4024), Expect = 0.0e+00
Identity = 785/1000 (78.50%), Postives = 875/1000 (87.50%), Query Frame = 0

Query: 1   MAEYFRVNPAEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLC 60
           MA+YFR +P EFSL STV+LQKAWYL TVLLDIG PASVEE+ VRC LFSVTPDFVRYLC
Sbjct: 1   MADYFRFDPDEFSLQSTVELQKAWYLFTVLLDIGHPASVEEIVVRCELFSVTPDFVRYLC 60

Query: 61  QIPDSPISLVDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYF 120
           +IP+SPISL DN+LV IS+VA+S+VGRYFSK TNG D  RR+FGVLE NR  GS+VKTYF
Sbjct: 61  EIPNSPISLADNSLVFISVVAISSVGRYFSKATNGWDSLRRHFGVLEPNRLWGSDVKTYF 120

Query: 121 RKRRRSMVDSGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTL 180
           RKR+RS++DSGVPF AKRMLTSTS  GNGS LSMT+RIS N  EVPAD +TL S NSLT+
Sbjct: 121 RKRKRSILDSGVPFPAKRMLTSTSGIGNGSCLSMTRRISQNFDEVPADYLTLTSSNSLTV 180

Query: 181 DLPFENPKMGHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKD------ 240
           +LPFE+ +MGHLDV  D  PF L YADFP+ LM  DG+MG +N  Q+SKI+IKD      
Sbjct: 181 NLPFEHLEMGHLDVKIDEEPFPLDYADFPNFLMSHDGRMGIDNDAQASKILIKDDAGLLS 240

Query: 241 ----DCHIHEVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYK 300
               DCHI+EVQKDS   N+ H D+GM CNLDLSLGPPCSS+NTL C KILD NNKLA +
Sbjct: 241 ENGHDCHINEVQKDSSIPNVRHADRGMLCNLDLSLGPPCSSTNTLPCDKILDNNNKLAIR 300

Query: 301 SGNTSVLCLGNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTK 360
           SGNTSV CLG DM P VQP EV+N + +++LSNI EWKLEDEKNKICE++N S++T+TT+
Sbjct: 301 SGNTSVSCLGGDMQPRVQPSEVENGICTKHLSNISEWKLEDEKNKICEMKNCSENTKTTE 360

Query: 361 NHLVHLEGHEITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVK 420
           NHLVHLEGH +TGEA ++FL LRNPV IS MD DDI RKG T+I CSLPDHL +Q+PSVK
Sbjct: 361 NHLVHLEGHAVTGEAGRNFLDLRNPVTISNMDRDDISRKGNTEIPCSLPDHLFDQQPSVK 420

Query: 421 TFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQ 480
           TF  LDGSQKC L  E+V MESAV+KEVSQ +EQQ+R N+  K  SK+QKFKK+SNENV 
Sbjct: 421 TFGELDGSQKCTLVPEKVLMESAVSKEVSQTLEQQNRYNSVHKPTSKVQKFKKSSNENVH 480

Query: 481 IKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVN 540
           IKENPLD+ TSS+KL++TSFPQFESF+IEEEEGSGGYGTVYRARRKND K IAIKCPHVN
Sbjct: 481 IKENPLDSPTSSLKLEKTSFPQFESFIIEEEEGSGGYGTVYRARRKNDGKRIAIKCPHVN 540

Query: 541 AHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRL 600
           AHK+++NNELKMLERFGGRNFIIK EG+FSS  + ECL+LEHVEHDRPEVLKKEIDIVRL
Sbjct: 541 AHKHNVNNELKMLERFGGRNFIIKCEGSFSSG-NSECLMLEHVEHDRPEVLKKEIDIVRL 600

Query: 601 QWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSN 660
           QWYGYCLFKAL  LHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+DLQ+KYSIASKSN
Sbjct: 601 QWYGYCLFKALACLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDLQQKYSIASKSN 660

Query: 661 TSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLK 720
           TS+HASSS +  PHS+SGS VKD+  FRGFA L+KGE GKSKQ  EHD+ LKKRA++PLK
Sbjct: 661 TSHHASSSHVTAPHSLSGSSVKDQN-FRGFAYLSKGETGKSKQACEHDQTLKKRAYIPLK 720

Query: 721 KYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRN 780
           KYPDMGGGSAVRSQGADGS ITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNA RN
Sbjct: 721 KYPDMGGGSAVRSQGADGSGITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNALRN 780

Query: 781 ADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEG 840
           +DH  QNSSDLRRKRIAAPPGKED+KIIHPSPIL+HCSGI VAGSR+ KAKGDA RKKEG
Sbjct: 781 SDHVTQNSSDLRRKRIAAPPGKEDNKIIHPSPILVHCSGISVAGSRVLKAKGDAKRKKEG 840

Query: 841 SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGS 900
           SCAGTKGFRAPEVLFRSLHQGPQVD+WSAGVTLLYLM+GRSPFTGDPEQN+KDIAKLRGS
Sbjct: 841 SCAGTKGFRAPEVLFRSLHQGPQVDIWSAGVTLLYLMVGRSPFTGDPEQNLKDIAKLRGS 900

Query: 901 EDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCL 960
           EDLWEVAKLHDRESAFP ELF+ITSFPA DLQSW+ +HTKRPDFLKLIP SLF+LVDKCL
Sbjct: 901 EDLWEVAKLHDRESAFPRELFDITSFPARDLQSWVKAHTKRPDFLKLIPTSLFNLVDKCL 960

Query: 961 TVNPRQRISAEEALKHEFFNSCHERLRKQRLLRRGSSSNS 990
           TVNPRQRI+AEEALKHEFFNSCH+RLRKQR+L RGS  NS
Sbjct: 961 TVNPRQRITAEEALKHEFFNSCHDRLRKQRMLLRGSRFNS 998

BLAST of Sed0025323 vs. ExPASy TrEMBL
Match: A0A6J1L0H4 (uncharacterized protein LOC111497968 OS=Cucurbita maxima OX=3661 GN=LOC111497968 PE=4 SV=1)

HSP 1 Score: 1540.0 bits (3986), Expect = 0.0e+00
Identity = 777/1000 (77.70%), Postives = 873/1000 (87.30%), Query Frame = 0

Query: 1   MAEYFRVNPAEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLC 60
           MA+YFR  P EFSLHSTV+LQKAWYL TVLLDIG PASVEE+ VRC LFSVTPDFVRYLC
Sbjct: 1   MADYFRFYPDEFSLHSTVELQKAWYLFTVLLDIGHPASVEEIVVRCELFSVTPDFVRYLC 60

Query: 61  QIPDSPISLVDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYF 120
           +IP+SPISL D++LV IS+VA+S+VGRYFSK TNG D  RR+F VLE NR  GS+VKTYF
Sbjct: 61  KIPNSPISLADSSLVFISVVAISSVGRYFSKATNGWDSLRRHFEVLEPNRLWGSDVKTYF 120

Query: 121 RKRRRSMVDSGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTL 180
           RKR+RS++DSGVPF AKRMLTSTS  GNGS LSMT+RISHN  EVPAD +TL S NSLT+
Sbjct: 121 RKRKRSILDSGVPFPAKRMLTSTSGIGNGSCLSMTRRISHNFDEVPADYLTLTSSNSLTV 180

Query: 181 DLPFENPKMGHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKD------ 240
           +LPFE+ + GHLDV  D  PF L YADFP+ LM  DG+MG +NAVQ+SKI+IKD      
Sbjct: 181 NLPFEHLETGHLDVKIDEEPFPLDYADFPNFLMSHDGRMGIDNAVQASKILIKDDAGFFS 240

Query: 241 ----DCHIHEVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYK 300
               DCHI+EVQK S   N+ H D+GM CNLDL+LGPPCSS+NTLSC KIL+ +NKLAY+
Sbjct: 241 ENGHDCHINEVQKVSSIPNVRHADRGMLCNLDLALGPPCSSTNTLSCDKILENHNKLAYR 300

Query: 301 SGNTSVLCLGNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTK 360
            GNTSV CLG DM P V+P EV+N + + +LSNI EWKLEDEKNKICE++N S++TRTT+
Sbjct: 301 CGNTSVSCLGGDMQPRVRPSEVENGICTMHLSNISEWKLEDEKNKICEMKNCSENTRTTE 360

Query: 361 NHLVHLEGHEITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVK 420
           NHLVHLEGHE+TGEA ++FL LRNPV  S MD DDI RKG+TQI CSLPDHL +Q+PSVK
Sbjct: 361 NHLVHLEGHEVTGEAGRNFLDLRNPVTFSNMDRDDISRKGKTQIPCSLPDHLFDQQPSVK 420

Query: 421 TFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQ 480
           TF  LDGSQKC L  +++ MESAV+KEVSQP+E Q++ N+  K  SK+QKFKKNSNENV 
Sbjct: 421 TFGELDGSQKCTLVPKKILMESAVSKEVSQPLEPQNQYNSVHKPTSKVQKFKKNSNENVH 480

Query: 481 IKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVN 540
           IKENPLD+ TSS+KL++TSFPQFESF+IEEEEGSGGYGTVYRARRKND K IAIKCPHVN
Sbjct: 481 IKENPLDSPTSSLKLEKTSFPQFESFIIEEEEGSGGYGTVYRARRKNDGKRIAIKCPHVN 540

Query: 541 AHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRL 600
           AHK+++NNELKMLERFGGRNFIIK EG+FSS  + ECL+LEHVEHDRPEVLKKEIDIVRL
Sbjct: 541 AHKHNVNNELKMLERFGGRNFIIKCEGSFSSG-NSECLMLEHVEHDRPEVLKKEIDIVRL 600

Query: 601 QWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSN 660
           QWYGYCLFKAL  LHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+DL +KYSIASKSN
Sbjct: 601 QWYGYCLFKALACLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDLHQKYSIASKSN 660

Query: 661 TSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLK 720
           TS+HASSS + VPHS+SGS VKD+  FRGFA L+KGE G SKQ  EHDK LKKRA++PLK
Sbjct: 661 TSHHASSSHVTVPHSLSGSSVKDQN-FRGFAYLSKGETGISKQACEHDKTLKKRAYIPLK 720

Query: 721 KYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRN 780
           KYPDMGGGSAVRSQGADGS ITSAKDVTSVRTLSTENMREPLPCQGRKELLSLV+NA RN
Sbjct: 721 KYPDMGGGSAVRSQGADGSGITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVKNALRN 780

Query: 781 ADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEG 840
           +DH  QNSSDLRRKRIAAPPGKED+KIIHPSPIL+HCSGI VAGSR+ KAKGDA RKKEG
Sbjct: 781 SDHVTQNSSDLRRKRIAAPPGKEDNKIIHPSPILVHCSGISVAGSRVLKAKGDAKRKKEG 840

Query: 841 SCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGS 900
           SCAGTKGFRAPEVLFRSLHQGPQVD+WSAGVTLLYLM+GRSPF GDPEQN+KDIAKLRGS
Sbjct: 841 SCAGTKGFRAPEVLFRSLHQGPQVDIWSAGVTLLYLMVGRSPFIGDPEQNLKDIAKLRGS 900

Query: 901 EDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCL 960
           EDLWEVAKLHDRESAFP ELF+ITSFPA DLQSW+ +HTKRPDF KLIP SLF+LVDKCL
Sbjct: 901 EDLWEVAKLHDRESAFPRELFDITSFPARDLQSWVKAHTKRPDFFKLIPTSLFNLVDKCL 960

Query: 961 TVNPRQRISAEEALKHEFFNSCHERLRKQRLLRRGSSSNS 990
           TVNPRQRI+AEEALKHEFF+SCH+RLRKQR+L +GS  NS
Sbjct: 961 TVNPRQRITAEEALKHEFFSSCHDRLRKQRMLLQGSRFNS 998

BLAST of Sed0025323 vs. ExPASy TrEMBL
Match: A0A6J1EYM1 (serine/threonine-protein kinase RIM15-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111439726 PE=4 SV=1)

HSP 1 Score: 1452.2 bits (3758), Expect = 0.0e+00
Identity = 745/1010 (73.76%), Postives = 844/1010 (83.56%), Query Frame = 0

Query: 10   AEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLCQIPDSPISL 69
            A +SLH+TV+LQKAWYL T+LLDIGRPASVEELAVRC LF  TPDFVRYLCQI  SPI +
Sbjct: 2    AHYSLHTTVELQKAWYLFTILLDIGRPASVEELAVRCELFRATPDFVRYLCQISASPICI 61

Query: 70   VDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYFRKRRRSMVD 129
            VD+ALV IS++A+SAVGRYFSK TNG  F RR+FGVLE NRF G +VKTYFRKR+RS++D
Sbjct: 62   VDDALVFISIIAVSAVGRYFSKATNGWGFLRRDFGVLEPNRFWGRDVKTYFRKRKRSVLD 121

Query: 130  SGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTLDLPFENPKM 189
            S   F  KR+LTSTS  GN   LS+T+RISHN AEVP   VTL S NSLT+DLPFE  K 
Sbjct: 122  SERHFHGKRILTSTSGIGNEICLSVTRRISHNYAEVPPSYVTLTSSNSLTIDLPFEKLKT 181

Query: 190  GHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKDDC----------HIH 249
            GH DV  D +PFSLGYAD P++LM    QMG++ AVQ+SK +IKDD           H+H
Sbjct: 182  GHFDVKIDEVPFSLGYADSPNLLMSHTRQMGSDIAVQASKTLIKDDAGPLRDNEHDRHMH 241

Query: 250  EVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYKSGNTSVLCL 309
            EVQKDS   NL H+D+GMSCNL LSL PPCSS+NTLS     DKN+K + K GN S LC 
Sbjct: 242  EVQKDSNMPNLRHSDRGMSCNLVLSLSPPCSSTNTLS----YDKNHKFSCKLGNASALCR 301

Query: 310  GNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTKNHLVHLEGH 369
            G+DM P VQP EVD+   +R+LSNI EW+LE+EKNKICE +NFSK TRTT+N LVHLEGH
Sbjct: 302  GDDMLPYVQPHEVDDRTCTRHLSNISEWELENEKNKICETRNFSKYTRTTENDLVHLEGH 361

Query: 370  EITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVKTFENLDGSQ 429
             +TGE   +FL LRNPV +S MDGD I RK  TQI CS+ D    +EP VKT   +DGSQ
Sbjct: 362  AVTGEVQINFLDLRNPVTMSNMDGDKISRKNLTQISCSIHDQPFIEEPPVKTLGEVDGSQ 421

Query: 430  KCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQIKENPLDTT 489
            KC  S E+V M SAVN+EVS P++QQ+R +N  KL SK+QKFKK SN NV IK+ PLD+T
Sbjct: 422  KCTSSPEKVLMGSAVNREVSHPLKQQNRYDNVHKLTSKVQKFKKISNGNVHIKKPPLDST 481

Query: 490  TSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVNAHKNSINNE 549
            + SM+L++T FPQFESF+IEEEEGSGGYGTVYRA+RK+D    AIKCPHVNAHK+++NNE
Sbjct: 482  SPSMELEKTLFPQFESFIIEEEEGSGGYGTVYRAQRKSDGIRKAIKCPHVNAHKHNVNNE 541

Query: 550  LKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFK 609
            LKMLERFGGRNFIIKYEG+FSS  + ECLILEHVEHDRPEVLKK+IDIV+LQWYGYCLFK
Sbjct: 542  LKMLERFGGRNFIIKYEGSFSSG-NNECLILEHVEHDRPEVLKKDIDIVQLQWYGYCLFK 601

Query: 610  ALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSNTSYHASSSR 669
            AL YLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+D+++KYSIASKSN+S+HASS  
Sbjct: 602  ALAYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDVRQKYSIASKSNSSHHASSDH 661

Query: 670  IPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLKKYPDMGGGS 729
            +PVPH VSGS VKD+  FR  ASL+K E GKSKQT+EH+KNLKKR +VPLKKYPDM GGS
Sbjct: 662  VPVPHPVSGSAVKDENNFRDLASLSKRETGKSKQTWEHNKNLKKRVYVPLKKYPDMRGGS 721

Query: 730  AVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRNADHAIQNSS 789
            A+RSQGADGS ITS KD TSVRT +TENMREPLPCQGRKELLSLVQNA RNAD A QNSS
Sbjct: 722  AIRSQGADGSGITSTKDATSVRTFATENMREPLPCQGRKELLSLVQNALRNADQATQNSS 781

Query: 790  DLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEGSCAGTKGFR 849
            DLRRKRIAAPPGKED+KIIHPSP+L+HC+GI VAGSR+ K+KGDA RK+EGSCAGTKGFR
Sbjct: 782  DLRRKRIAAPPGKEDNKIIHPSPMLVHCTGISVAGSRVLKSKGDAKRKREGSCAGTKGFR 841

Query: 850  APEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGSEDLWEVAKL 909
            APEVLFRSLHQGPQVDVWSAGVTLLYLM+GRSPFTGDPEQNIKDIA+LRGSEDLWEVAKL
Sbjct: 842  APEVLFRSLHQGPQVDVWSAGVTLLYLMVGRSPFTGDPEQNIKDIAQLRGSEDLWEVAKL 901

Query: 910  HDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCLTVNPRQRIS 969
            HDRESAFPGELFNI SFPAMDLQSW+  HTKR DF KLIPRSLFDLVDKCLTVNPRQRI+
Sbjct: 902  HDRESAFPGELFNIKSFPAMDLQSWVKVHTKRLDFFKLIPRSLFDLVDKCLTVNPRQRIT 961

Query: 970  AEEALKHEFFNSCHERLRKQRLLRRGSSSNS---------VQGQSFEILR 1000
            A+EAL+HEFFNSCH++LRKQR+LRRGSS NS         VQGQSFE+LR
Sbjct: 962  ADEALRHEFFNSCHDKLRKQRILRRGSSLNSADVLGESKAVQGQSFELLR 1006

BLAST of Sed0025323 vs. ExPASy TrEMBL
Match: A0A6J1F3T6 (serine/threonine-protein kinase RIM15-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439726 PE=4 SV=1)

HSP 1 Score: 1444.5 bits (3738), Expect = 0.0e+00
Identity = 745/1020 (73.04%), Postives = 844/1020 (82.75%), Query Frame = 0

Query: 10   AEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLCQIPDSPISL 69
            A +SLH+TV+LQKAWYL T+LLDIGRPASVEELAVRC LF  TPDFVRYLCQI  SPI +
Sbjct: 2    AHYSLHTTVELQKAWYLFTILLDIGRPASVEELAVRCELFRATPDFVRYLCQISASPICI 61

Query: 70   VDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYFRKRRRSMVD 129
            VD+ALV IS++A+SAVGRYFSK TNG  F RR+FGVLE NRF G +VKTYFRKR+RS++D
Sbjct: 62   VDDALVFISIIAVSAVGRYFSKATNGWGFLRRDFGVLEPNRFWGRDVKTYFRKRKRSVLD 121

Query: 130  SGVPFAAKRMLTSTS----------ETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLT 189
            S   F  KR+LTSTS            GN   LS+T+RISHN AEVP   VTL S NSLT
Sbjct: 122  SERHFHGKRILTSTSGIVRPKMIDGVVGNEICLSVTRRISHNYAEVPPSYVTLTSSNSLT 181

Query: 190  LDLPFENPKMGHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKDDC--- 249
            +DLPFE  K GH DV  D +PFSLGYAD P++LM    QMG++ AVQ+SK +IKDD    
Sbjct: 182  IDLPFEKLKTGHFDVKIDEVPFSLGYADSPNLLMSHTRQMGSDIAVQASKTLIKDDAGPL 241

Query: 250  -------HIHEVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAY 309
                   H+HEVQKDS   NL H+D+GMSCNL LSL PPCSS+NTLS     DKN+K + 
Sbjct: 242  RDNEHDRHMHEVQKDSNMPNLRHSDRGMSCNLVLSLSPPCSSTNTLS----YDKNHKFSC 301

Query: 310  KSGNTSVLCLGNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTT 369
            K GN S LC G+DM P VQP EVD+   +R+LSNI EW+LE+EKNKICE +NFSK TRTT
Sbjct: 302  KLGNASALCRGDDMLPYVQPHEVDDRTCTRHLSNISEWELENEKNKICETRNFSKYTRTT 361

Query: 370  KNHLVHLEGHEITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSV 429
            +N LVHLEGH +TGE   +FL LRNPV +S MDGD I RK  TQI CS+ D    +EP V
Sbjct: 362  ENDLVHLEGHAVTGEVQINFLDLRNPVTMSNMDGDKISRKNLTQISCSIHDQPFIEEPPV 421

Query: 430  KTFENLDGSQKCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENV 489
            KT   +DGSQKC  S E+V M SAVN+EVS P++QQ+R +N  KL SK+QKFKK SN NV
Sbjct: 422  KTLGEVDGSQKCTSSPEKVLMGSAVNREVSHPLKQQNRYDNVHKLTSKVQKFKKISNGNV 481

Query: 490  QIKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHV 549
             IK+ PLD+T+ SM+L++T FPQFESF+IEEEEGSGGYGTVYRA+RK+D    AIKCPHV
Sbjct: 482  HIKKPPLDSTSPSMELEKTLFPQFESFIIEEEEGSGGYGTVYRAQRKSDGIRKAIKCPHV 541

Query: 550  NAHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVR 609
            NAHK+++NNELKMLERFGGRNFIIKYEG+FSS  + ECLILEHVEHDRPEVLKK+IDIV+
Sbjct: 542  NAHKHNVNNELKMLERFGGRNFIIKYEGSFSSG-NNECLILEHVEHDRPEVLKKDIDIVQ 601

Query: 610  LQWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKS 669
            LQWYGYCLFKAL YLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+D+++KYSIASKS
Sbjct: 602  LQWYGYCLFKALAYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDVRQKYSIASKS 661

Query: 670  NTSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPL 729
            N+S+HASS  +PVPH VSGS VKD+  FR  ASL+K E GKSKQT+EH+KNLKKR +VPL
Sbjct: 662  NSSHHASSDHVPVPHPVSGSAVKDENNFRDLASLSKRETGKSKQTWEHNKNLKKRVYVPL 721

Query: 730  KKYPDMGGGSAVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARR 789
            KKYPDM GGSA+RSQGADGS ITS KD TSVRT +TENMREPLPCQGRKELLSLVQNA R
Sbjct: 722  KKYPDMRGGSAIRSQGADGSGITSTKDATSVRTFATENMREPLPCQGRKELLSLVQNALR 781

Query: 790  NADHAIQNSSDLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKE 849
            NAD A QNSSDLRRKRIAAPPGKED+KIIHPSP+L+HC+GI VAGSR+ K+KGDA RK+E
Sbjct: 782  NADQATQNSSDLRRKRIAAPPGKEDNKIIHPSPMLVHCTGISVAGSRVLKSKGDAKRKRE 841

Query: 850  GSCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRG 909
            GSCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLM+GRSPFTGDPEQNIKDIA+LRG
Sbjct: 842  GSCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMVGRSPFTGDPEQNIKDIAQLRG 901

Query: 910  SEDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKC 969
            SEDLWEVAKLHDRESAFPGELFNI SFPAMDLQSW+  HTKR DF KLIPRSLFDLVDKC
Sbjct: 902  SEDLWEVAKLHDRESAFPGELFNIKSFPAMDLQSWVKVHTKRLDFFKLIPRSLFDLVDKC 961

Query: 970  LTVNPRQRISAEEALKHEFFNSCHERLRKQRLLRRGSSSNS---------VQGQSFEILR 1000
            LTVNPRQRI+A+EAL+HEFFNSCH++LRKQR+LRRGSS NS         VQGQSFE+LR
Sbjct: 962  LTVNPRQRITADEALRHEFFNSCHDKLRKQRILRRGSSLNSADVLGESKAVQGQSFELLR 1016

BLAST of Sed0025323 vs. ExPASy TrEMBL
Match: A0A6J1ICH5 (uncharacterized protein LOC111472476 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111472476 PE=4 SV=1)

HSP 1 Score: 1442.6 bits (3733), Expect = 0.0e+00
Identity = 744/1010 (73.66%), Postives = 839/1010 (83.07%), Query Frame = 0

Query: 10   AEFSLHSTVDLQKAWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLCQIPDSPISL 69
            A++SLHSTV+LQKAWYL T+LLDIGRPASVEELAVRC LF  TPDFVRYLCQI  SPI +
Sbjct: 2    ADYSLHSTVELQKAWYLFTILLDIGRPASVEELAVRCELFRATPDFVRYLCQISGSPICI 61

Query: 70   VDNALVCISMVALSAVGRYFSKMTNGCDFSRRNFGVLESNRFCGSEVKTYFRKRRRSMVD 129
            VD+ALV IS++A+SAVGRYFSK TNG  F RR+FGVLE NRF G +VKTYFRKR+RS++D
Sbjct: 62   VDDALVFISIIAVSAVGRYFSKATNGWGFLRRDFGVLEPNRFWGRDVKTYFRKRKRSVLD 121

Query: 130  SGVPFAAKRMLTSTSETGNGSHLSMTKRISHNLAEVPADNVTLMSLNSLTLDLPFENPKM 189
            S   F  KR+LTSTS  GN S LS T+RISHN AEVPA  VTL S NSLT+DLPFE  K 
Sbjct: 122  SEGHFHGKRILTSTSGIGNESCLSTTRRISHNYAEVPASYVTLTSSNSLTVDLPFEKLKT 181

Query: 190  GHLDVNFD-MPFSLGYADFPSVLMRQDGQMGAENAVQSSKIMIKDDC----------HIH 249
            GH DV  D +PFSLGYAD P++LM    QMG++ AVQ+ K + KDD           H+H
Sbjct: 182  GHFDVKIDEVPFSLGYADSPNLLMSHTRQMGSDIAVQAIKTLTKDDAGPLRDNEHDRHMH 241

Query: 250  EVQKDSRTQNLCHTDKGMSCNLDLSLGPPCSSSNTLSCHKILDKNNKLAYKSGNTSVLCL 309
            EVQKDS   NL ++D+GMSCNL LSL PPCSS+NTLS     DKN+K + K GN S LC 
Sbjct: 242  EVQKDSNMPNLRYSDRGMSCNLVLSLSPPCSSTNTLS----YDKNHKFSCKLGNASALCR 301

Query: 310  GNDMPPSVQPPEVDNVVFSRNLSNILEWKLEDEKNKICEVQNFSKSTRTTKNHLVHLEGH 369
            G+DM P VQP EVD+   +R+LSNI EW+LEDEKNKICE +NFSK TRTT N LVHLEGH
Sbjct: 302  GDDMLPYVQPHEVDDGTCTRHLSNISEWELEDEKNKICETRNFSKYTRTTGNDLVHLEGH 361

Query: 370  EITGEATKDFLGLRNPVNISKMDGDDIPRKGETQILCSLPDHLNNQEPSVKTFENLDGSQ 429
             +TGE   +FL LRNPV IS MDGD I RK  TQI CS+PD     E  VK    +DGSQ
Sbjct: 362  AVTGEVQINFLELRNPVTISNMDGDKISRKNVTQISCSIPDQPFIVELPVKILGEVDGSQ 421

Query: 430  KCNLSLEEVFMESAVNKEVSQPVEQQDRSNNDPKLMSKIQKFKKNSNENVQIKENPLDTT 489
            KC  S E+V M SAVN+EVS P++QQ+R +N  KL  K QKFKK SN NV IK+ P+D+T
Sbjct: 422  KCTSSPEKVLMASAVNREVSHPLKQQNRYDNVHKLTPKAQKFKKISNGNVHIKKPPVDST 481

Query: 490  TSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVNAHKNSINNE 549
            + SM+L++T FPQFESF+IEEEEGSGGYGTVYRA+RK+D    AIKCPHVNAHK+++NNE
Sbjct: 482  SPSMELEKTLFPQFESFIIEEEEGSGGYGTVYRAQRKSDGIRKAIKCPHVNAHKHNVNNE 541

Query: 550  LKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFK 609
            LKMLERFGGRNFIIKYEG+FSS  + ECLILEHVEHDRPEVLKK+ID+V+LQWYGYCLFK
Sbjct: 542  LKMLERFGGRNFIIKYEGSFSSG-NNECLILEHVEHDRPEVLKKDIDVVQLQWYGYCLFK 601

Query: 610  ALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAVDLQKKYSIASKSNTSYHASSSR 669
            AL YLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLA+DL++KYSIASKSN+S+HASS  
Sbjct: 602  ALAYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAMDLRQKYSIASKSNSSHHASSDH 661

Query: 670  IPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEHDKNLKKRAFVPLKKYPDMGGGS 729
            +PVPH VSGS VKD+  FR  ASL+K E GKSKQT+EH+KNLKKR +VPLKKYPDM GGS
Sbjct: 662  VPVPHPVSGSAVKDENNFRDLASLSKRETGKSKQTWEHNKNLKKRVYVPLKKYPDMRGGS 721

Query: 730  AVRSQGADGSVITSAKDVTSVRTLSTENMREPLPCQGRKELLSLVQNARRNADHAIQNSS 789
            A+RSQGADGS ITSAKDVTSVRT +TENMREPLPCQGRKELLSLVQNA +NAD A QNSS
Sbjct: 722  AIRSQGADGSGITSAKDVTSVRTFATENMREPLPCQGRKELLSLVQNALQNADQATQNSS 781

Query: 790  DLRRKRIAAPPGKEDSKIIHPSPILMHCSGIPVAGSRIPKAKGDAMRKKEGSCAGTKGFR 849
            DLRRKRIAAPPGKED+KIIHPSP+L+HC+ I VAGSR+ K+KGDA RK+EGSCAGTKGFR
Sbjct: 782  DLRRKRIAAPPGKEDNKIIHPSPMLVHCTDISVAGSRVLKSKGDAKRKREGSCAGTKGFR 841

Query: 850  APEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPFTGDPEQNIKDIAKLRGSEDLWEVAKL 909
            APEVLFRSLHQGPQVDVWSAGVTLLYLM+GRSPFTGDPEQNIKDIA+LRGSEDLWEVAKL
Sbjct: 842  APEVLFRSLHQGPQVDVWSAGVTLLYLMVGRSPFTGDPEQNIKDIAQLRGSEDLWEVAKL 901

Query: 910  HDRESAFPGELFNITSFPAMDLQSWIMSHTKRPDFLKLIPRSLFDLVDKCLTVNPRQRIS 969
            HDRESAFPGELFNI SFPAMDLQSW+  HTKR DF KLIPRSLFDLVDKCLTVNPRQRI+
Sbjct: 902  HDRESAFPGELFNIKSFPAMDLQSWVKVHTKRLDFFKLIPRSLFDLVDKCLTVNPRQRIT 961

Query: 970  AEEALKHEFFNSCHERLRKQRLLRRGSSSNS---------VQGQSFEILR 1000
            A+EAL+HEFFNSCHE+LRK R+LRRGSS NS         VQGQSFE+LR
Sbjct: 962  ADEALRHEFFNSCHEKLRKLRILRRGSSLNSADVLGVSKAVQGQSFELLR 1006

BLAST of Sed0025323 vs. TAIR 10
Match: AT4G16970.1 (Protein kinase superfamily protein )

HSP 1 Score: 529.6 bits (1363), Expect = 5.3e-150
Identity = 286/531 (53.86%), Postives = 368/531 (69.30%), Query Frame = 0

Query: 457 IQKFKKNSNENVQIKENPLDTTTSSMKLKETSFPQFESFVIEEEEGSGGYGTVYRARRKN 516
           IQK K+N N  V++K+          + K  + P F+S+ I EEEGSGGYG VY+A RK 
Sbjct: 371 IQKRKQNFN-TVRLKD-------QKDQAKHNTIPDFDSYTIVEEEGSGGYGIVYKATRKT 430

Query: 517 DDKHIAIKCPHVNAHKNSINNELKMLERFGGRNFIIKYEGAFSSSEHRECLILEHVEHDR 576
           D    AIKCPHV A K  +NNE++MLERFGG+N IIK+EG   + +  +C+ILEH+EHDR
Sbjct: 431 DGTEFAIKCPHVGAQKYYVNNEIRMLERFGGKNCIIKHEGCLKNGD-SDCIILEHLEHDR 490

Query: 577 PEVLKKEIDIVRLQWYGYCLFKALTYLHKQGVMHRDVKPGNFLFSRKQNKGYLIDFNLAV 636
           P+ LK+EID+ +LQWYGYC+FKAL+ LHKQGV+HRDVKPGNFLFSRK NKGYLIDFNLA+
Sbjct: 491 PDSLKREIDVYQLQWYGYCMFKALSSLHKQGVVHRDVKPGNFLFSRKTNKGYLIDFNLAM 550

Query: 637 DLQKKYSIASKSNTSYHASSSRIPVPHSVSGSVVKDKKIFRGFASLNKGEAGKSKQTFEH 696
           DL +KY  A KS      ++S +P       ++VK         ++N+G    S++T   
Sbjct: 551 DLHQKYRRADKSK-----AASGLPTASKKHHTLVK------SLDAVNRGTNKPSQKTLA- 610

Query: 697 DKNLKKRAFVPLKKYPDMGGGSAVRSQGADGSVITSAKDVTSVR-TLSTENMREPLPCQG 756
             N  K+A    +   DM     + SQGA+GS +TSAKDVTS R   S E  REPLPC G
Sbjct: 611 -PNSIKKAAGKTRARNDMTRWERLNSQGAEGSGLTSAKDVTSTRNNPSGEKRREPLPCHG 670

Query: 757 RKELLSLVQNARR--NADHAIQNSSDL-RRKRIAAPPGKEDSKIIHPSPILMHCSGIPVA 816
           RK LL  +Q        +H + + +    RKR+AA PGK + ++++ +P+ +  +G P A
Sbjct: 671 RKALLDFLQETMSVPIPNHEVSSKAPTSMRKRVAALPGKAEKELLYLTPMPLCSNGRPEA 730

Query: 817 GSRIPKAKGDAMRKKEGSCAGTKGFRAPEVLFRSLHQGPQVDVWSAGVTLLYLMIGRSPF 876
                   GD + KK+G C+GTKGFRAPEV FRSLHQGP++DVWSAGVTLLYL++GR+PF
Sbjct: 731 --------GDVIEKKDGPCSGTKGFRAPEVCFRSLHQGPKIDVWSAGVTLLYLIMGRTPF 790

Query: 877 TGDPEQNIKDIAKLRGSEDLWEVAKLHDRESAFPGELFNITSFPAMDLQSWIMSHTKRPD 936
           TGDPEQNIKDIA+LRGSE+LWEVAKLH+RES+FP EL+       M+L+ W   +TKR +
Sbjct: 791 TGDPEQNIKDIAQLRGSEELWEVAKLHNRESSFPKELYESRYLKGMELRKWCELNTKRRE 850

Query: 937 FLKLIPRSLFDLVDKCLTVNPRQRISAEEALKHEFFNSCHERLRKQRLLRR 984
           FL +IP SL DLVDKCLTVNPR+RISAE+ALKH+FF+  HE LR Q LL++
Sbjct: 851 FLDVIPLSLLDLVDKCLTVNPRRRISAEDALKHDFFHPVHETLRNQMLLKQ 871

HSP 2 Score: 50.4 bits (119), Expect = 9.4e-06
Identity = 23/50 (46.00%), Postives = 34/50 (68.00%), Query Frame = 0

Query: 23 AWYLVTVLLDIGRPASVEELAVRCGLFSVTPDFVRYLCQIPDSPISLVDN 73
          A+ L  +LL  G P SV +L+ +C  F  +P+ V+YLC IP+SPISL ++
Sbjct: 39 AFELSRLLLSSGHPESVIDLSSKCTYFQGSPNLVKYLCSIPNSPISLAED 88

BLAST of Sed0025323 vs. TAIR 10
Match: AT2G23070.1 (Protein kinase superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 8.8e-12
Identity = 44/144 (30.56%), Postives = 69/144 (47.92%), Query Frame = 0

Query: 493 ESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVNAHKNSINNELKMLERF-GGRNFI 552
           + + +  + G G Y  V+      D++   IK       K  I  E+K+L+   GG N +
Sbjct: 130 DDYEVVRKVGRGKYSEVFEGIHATDNEKCVIKILK-PVKKKKIKREIKILQNLCGGPNIV 189

Query: 553 IKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFKALTYLHKQGVMHR 612
              +           LI EHV +   +VL   +    +++Y + L KAL + H +G+MHR
Sbjct: 190 KLLDIVRDQQSKTPSLIFEHVNNKDFKVLYPTLSDYDVRYYIFELLKALDFCHSRGIMHR 249

Query: 613 DVKPGNFLFSRKQNKGYLIDFNLA 636
           DVKP N +   +Q K  LID+ LA
Sbjct: 250 DVKPHNVMIDHEQRKLRLIDWGLA 272

BLAST of Sed0025323 vs. TAIR 10
Match: AT2G23080.2 (Protein kinase superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 1.5e-11
Identity = 44/144 (30.56%), Postives = 68/144 (47.22%), Query Frame = 0

Query: 493 ESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVNAHKNSINNELKMLERF-GGRNFI 552
           + + +  + G G Y  V+  +  N ++   IK       K  I  E+K+L+   GG N +
Sbjct: 32  DDYEVVRKVGRGKYSEVFEGKNVNTNERCVIKILK-PVKKKKIKREIKILQNLCGGPNIV 91

Query: 553 IKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFKALTYLHKQGVMHR 612
             Y+           L+ E V     +VL   +    +++Y Y L KAL + H QG+MHR
Sbjct: 92  KLYDIVRDEHSKTPSLVFEFVNSVDFKVLYPTLTDYDIRYYIYELLKALDFCHSQGIMHR 151

Query: 613 DVKPGNFLFSRKQNKGYLIDFNLA 636
           DVKP N +   +  K  LID+ LA
Sbjct: 152 DVKPHNVMIDHQLRKLRLIDWGLA 174

BLAST of Sed0025323 vs. TAIR 10
Match: AT2G23080.1 (Protein kinase superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 1.5e-11
Identity = 44/144 (30.56%), Postives = 68/144 (47.22%), Query Frame = 0

Query: 493 ESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVNAHKNSINNELKMLERF-GGRNFI 552
           + + +  + G G Y  V+  +  N ++   IK       K  I  E+K+L+   GG N +
Sbjct: 32  DDYEVVRKVGRGKYSEVFEGKNVNTNERCVIKILK-PVKKKKIKREIKILQNLCGGPNIV 91

Query: 553 IKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFKALTYLHKQGVMHR 612
             Y+           L+ E V     +VL   +    +++Y Y L KAL + H QG+MHR
Sbjct: 92  KLYDIVRDEHSKTPSLVFEFVNSVDFKVLYPTLTDYDIRYYIYELLKALDFCHSQGIMHR 151

Query: 613 DVKPGNFLFSRKQNKGYLIDFNLA 636
           DVKP N +   +  K  LID+ LA
Sbjct: 152 DVKPHNVMIDHQLRKLRLIDWGLA 174

BLAST of Sed0025323 vs. TAIR 10
Match: AT3G50000.1 (casein kinase II, alpha chain 2 )

HSP 1 Score: 67.8 bits (164), Expect = 5.7e-11
Identity = 44/144 (30.56%), Postives = 68/144 (47.22%), Query Frame = 0

Query: 493 ESFVIEEEEGSGGYGTVYRARRKNDDKHIAIKCPHVNAHKNSINNELKMLERF-GGRNFI 552
           + + +  + G G Y  V+     N+++   IK       K  I  E+K+L+   GG N +
Sbjct: 102 DDYEVVRKVGRGKYSEVFEGINMNNNEKCIIKILK-PVKKKKIRREIKILQNLCGGPNIV 161

Query: 553 IKYEGAFSSSEHRECLILEHVEHDRPEVLKKEIDIVRLQWYGYCLFKALTYLHKQGVMHR 612
              +           LI E+V     +VL   +    +++Y Y L KAL + H QG+MHR
Sbjct: 162 KLLDVVRDQHSKTPSLIFEYVNSTDFKVLYPTLTDYDIRYYIYELLKALDFCHSQGIMHR 221

Query: 613 DVKPGNFLFSRKQNKGYLIDFNLA 636
           DVKP N +   +  K  LID+ LA
Sbjct: 222 DVKPHNVMIDHELRKLRLIDWGLA 244