Lsi04G009750 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G009750
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGDSL esterase/lipase
Locationchr04: 10348112 .. 10355510 (+)
RNA-Seq ExpressionLsi04G009750
SyntenyLsi04G009750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGAAGAATTTAAGAATAAATGTGGTGTTTTTAAAAACAAAATTTTAACAAACAGTTTGTAGGGGAAAGGTAGGAATGTGTAGCGGTGGGAGGTAAAGAAATGGCAAAACGCAACAAAATCAGCGGTTTATAAAATGAAGCGAAATGAATGCTTTTAGAGGCAAAAAGCTACAACTGCAAGAAACCCCTTTAAGGCCTTTTCAAATTTCATTTTCAATTTGGTCTTCGTTTCTCTCTCTCTTGAGGTTGTGGTTGCAGCCTTAACTTCATCCGAGCAAGAGGGAGTGAAAATCGGCGGTTCGTCCTTTTTTTTGTTTTTCCCATTTCTTCCTTTTTGATGTATCTGTTTGATTGAGTTACAACAGACATGGTAGGACCTTCGAGACCTCAGTTTGTTCTTTTTGGATCCTCCATTACTCAGTTCAGCTTCTCCAATGGCGGTTGGGGTGCTATTCTTGCTGATGTTTATGCTCGCAAAGTAACCCTTCTTTCTCTCTCTCTGTTTTTTTATTTTTTCCCCTGGTTTTGTTTTGTTGTTTTTGAAATTTTTGACCCATATGCTAATCTTTGAATGCCCACATGGGAAACCCCACTAATTTGATTCTTTTTTGTTTTTGTTGCTCTGTGATCTGTTGAATACCTGAAATGAAGGAAGATTGTACATGGAAGCCCTTTTACTTCATTTGGATTTTGATGGTTTCTCTGAAAACATAATTTGTTTTCTGTTTTTCTGTTTTTATTATTATTATTATTATTATTATTATTTTGTTGGGTTGGTATAGTGGCGACGGCCAACCGGTTAGAGTAAATGAGTTGGCTACCAAATGTGGTAGGCACTGTTCTCTCGTGAGAATAGTTAGGCTGGACATTCCAGGACATCAAAATCAAAGAGTTTTCTGTTGATTTTTGGTTAAAAACACGTTCCTAAACTTTCTTGATGATAGCACTTTAGTCACTAAATTTTGGTTTGTAGTGATTTATTAGTCTATGTATTTTCAATTTTATAACAATTTGGTCTATATATTTTTCAAATATTTAACAATTTAAACTCTGCAGTAATTTTTCTTTTAGTCAAAATTAAATTTCAATTTTTATAATTTAACAATGTAGATTTTGTAGCTTATAAACATACTGGGTCCCTAATTAGTTCATTGGTCTAATCTATGCGAGAAAACTCATTAAATCTTAATACCAATTTTCTTGATAGGGACTAAATTGTTACAACTTTTAGAGTATAAGGGTCAAATTGTTGTAAAGTTTAAATTTAAGGACTAAATTATTACAAAATTGGAATTATAGGGACTAAATCATTACAAACTAAAGTTCAGAGGCCGAAATGTTTCTTTCACAAAAGTTCGGGACCAAAAGTGTTTTTTAGTCCATCTTTTTTTCTTTTTTCCTTTTTTCCTTTCTGGTTGTAGGGAATTGGATTTATGGTCTGATTCTTTTGTTCATTGTAATGGTATTTTGCAGCCTTCTTACAAGGTTCATTGAGTGTCTAACATAGTCATTTCAAACTCATCTTAAATATCAGGACAGTCTGAATCTTGAGAATTCGTTGTTAACTTTTCTTGCTTTCTCACTTCATAGAGGTGGGATTTTCTCAACCTGCAGAACGTTCTTCCTTTTGTTGATGTATTTTAGAAAGAAAAAAAAGGGTGCAACTTTTCTAACTTAAAACTCTTTATACTGAAAATTGCTTTGTGCAAGTCTTGGTCAATATTGATTTTTCCAAAATAGGATTGGAGATTCGAACCATTGGACCTTTTTGTCGAGAATATAATACCTTAACTAGTTGAGCTATGCTCAAGTTGGCAATGTAAATTATCATTTTTCTAACACAAATGTCTTGATTATGAAATTGTGCAAGTCTTGGTCAATGTTGCTGCTGCTCTATTTATTAAAAAAGTAAAGAACTTTAGGATAAAAGTTATAAGATTGATACCACATTCATCAAAAAGGTATGGACATCTCTTTTAGGTTGCACGCCACTTTGGATGGTATATTTGTGCTTGAAAAGACTCCACCAGTCTACTCTGTTTGTGCTTGAAAAGACTCCATCAGGCTAGGACACTCTGATGGAATTAAAAAGTTCCAAATTACCTATTTTATGCAATTTTATACAGTTTCTTATTTTGTTGGGTTGGGTTTCATTCTGTTAAGGCTTGTTTTAAAAGCCCATATAACTTGAGACCTGATGTAAACCTGTTGTGTTGGGCCCTTATGCAAAGGGAAGACTTTTCCTTTCATTTATATACCATTTTGACTAAATATTTACAAATTTATTTGCCATAATGAAATATTTACATATCTGTTGTCTTTCAGTATTTTATTCTATATTTTTCCCCCTTATTGTCCATGGTATGATTAGATTTTTATTTTTGAAATATGGTTGGTTTCTTTCCTAAAATGTCATATTTAACGAATAGAATCTTTTTCTTCTTACTTTTTCCACTGTGAAAAAAATTCTCTGTATAGAAGCCAACATTGTCGTCGCTTTCAAAAGTATTCTCAGTTCACACCAAAGATTAAAGAACCATCTGTATTCTCCTGCACTAATTACTTCCTTTTGTAGAGTAGAATTTTAAATTCTAAATAAAGTTAAAATATTATATTTTCCCTATAATCTTTTCTTATTTATTTTCTGCTTCCATACAAGATATTTCTATTTCTATATAAAAAATTAGAATCTCCAGATTCTCTAACACTAGTTTCCCACAGTCCCTCCCAATTCAAGTGGTATATGTGTGTTATAGTTTTGAAGTGCATGTAGATTTGTAGCTATTTGGTACGATCTGGTATACTTTTGTAAATATTCCTTTCTTTTATTGAGTTCAATAATATTTAGATCTTAGTAAGTGGATTACGTTAAAGTTTAACCTCCCATAGATCAAATCATGAGCTCTCTTAGATCCTTAGTTTCACTTGATTAAGTCAGATTTTTGCCCTCCCCTGATGAAGAGAGTTATTATTTATGTTACATTAATCACCTTTTCCAATAAAGCCTATCAAATACTCCTTTGTCATTGCCTCTTTTTTCATTTGCATTTTAAATTTTAAATGGGTAGAAATCCTTAATCTCTAGTAATAGAAGTGAGATGATCTAAGATGGGGAATGAGCATGAAATGATGGCAGTTGAAAAGGCTTTCAGACAGAAAGCAGAGGTGTATGTTTCAGACTGAAATTAAATTACTTCTATTTTTAAGTTGAATTTGTTGGTCAGTTTGAATTCTATACGTGCCTTGTTGCATGATTTGTGACAATGTTATATTGAATCTGTTTTCTTCAGGCAGATATTCTTCTACGAGGTTACTTAAGTTGGAATTCTCGGCGAGCGGTACAGGTTCTTGATCAGATTTTTCCAAAGGTATGGTGTCAAGTGTTTTAGAATTTGGATGTCTGTTTTGGTTTTAGTGTTTGCTGGATATCTTTATCCTTTAGAATGAAGTATGTGGTTGTCTGCCTTTTTCCTCTTGTATAAATCACTAAAAGTTGTGCTTATGACTTATATATTATTAAAAGTTATAGTAAAATTGTTTGTCTATTTATTGTGTTATATAATAAATAAAGTTTAATATCATGTGAATAACATACGAATTTAGTATTTATATGACTGATTTTGAATAATGTTATCTGAGTTAACATATCTTAATTTTTTGGGATGTTTTAAAATATATAGTTATATGATTATAACACCAAAGGTACAGGTTTGACAATAAAATTCTTTTATCTGATAGGAATGCTGCAAAAGCAAAACCACTTTTCTTTGTTGGAATAAATGATAGAGATGGATGTATATGTGAATGTTGCTCGGTTACACATGTATGCATAAATTCATGCATATATATATATATATATATATATACATGCATGCGCGCACACACATACACACATACATATTTAAGGTTTAAAATGTTGATGTTGATGAAAATGTTAAGGTTTCAATTTTATAGAAATGTCGATGAAAATATTGATTTTGATGGATAATCTTGAAAAAGTAATAAAAACATAAAGATTTAAAAGAATTATTTAAACTAGTAAATAAACATTTTACATTTTATTGATGAGTTAAAATGTTTATTATTTATATTATATTCACATTTTGTTGCTTTTTCTTATGTTGCATGAATTTGTTTTTGCAATATAATGGATCTACATGTCGATTGAAATTTAATACCTTGTGTGTGTGTGTGACAAACAAGAAATTTTCATTAAATTTAGAAAGGATATATAAAAGATAAATAATAAAATATAAAAGAAGGAAGTAGGAAGGAAAAAACAAAAGAGGGATTACAAAAAGGCCCTCAAATTGTTCAAAAGTAAGAAAGAGAATAGTTTAAAAAAAAAACTTTGGATAAGAGACTCCAGCTAAAGTGGAAATGGATTACAACCCAAGAGAAGTCAAAATTCCTTCCACTATTTTGGAACGTTCCTTTATTCTTCTCCATCAAAAGCTCCCATAGAACAGAAAAAGAAAAAAGAAAAATAAAAAATAGGATTAACCTATAAAACTGAAGACATGGTTACTAATGGTATTCTTCTGCTTAAATACTTAGAATGCTACTTATTTTTTCCTTTCTTTTTCCTTGAAATCATAAATATTTGGGGCAACTTATGTATACCTCGACTATTCTCACTGAAAACTGTTTGAAATTATATTTGATTGTAAGAGAAGTGGTAGGATTTTTAATATCCCAAGTAGATGCCTATGTGGGAGATTATATTTTCATGTATATTGTCTCAAAGAAAACTTTGCTACATTCAACTATTTAGGCAATGGTACAGCTAGAACTTGTTGCGTTTGTAAAAGTCCTAGACTTGTCATTGTGATTATTTTGGCAAGAATCAAGATACTGGAAAAAAATGTACAAAAATTATAACCCTCACTTATTGATCTACACAATAACCCATGTCAAGAAAAGAAAAAAGGGGAAAGGAGATTTATAAATATTTGATAATATCCCCAGGAAGGTGCGGGTGTGGAATGAACAAAAGAGAACTGGCCCCAATAATATCACAACCATACCTCCTAATCACAATTCCTAGCTCCATTAGCTGGATGGAAATGCCAAAAGCTAGGACACTTTCATAGGGGATGGCATTTCACAATCAGTTCTTATACTCATTAATGATTTGCTTGTGTTGCCATTGAATATACATTATCCAGTGGGCAGAGTTCTGCAAGTAGATAAATTGGAACTGTAAATAATCTGTAGAAAATCTCAGACTGATAGAACTTGATATTATTTATCAACAGAGTACAAACTCAGGGAGAATTAGAAAGCTCCCCCTTCTCTCCTAGACTTGACTATAGGAGGTGTGTTTGGATTGACTTTTTAAGTGTTTAAATAAGTGTTTCTAAGTACAAAAAAAAAAACGTTTATAAACACTTAGAAAGTCAATCCAAACAGGCCCCACCTCAGTTCACTAAGGCGCACCTTCTTTTCTTGCTTGCATCCTCCAAATACCTCTTAAGTCACATAGTGACAGCTTTCGTTTCAATTCTTATTAAGGAATTTGAATTATCTAGGTCTGAGGCTGATCTGTGTATTCTAGATATGTTAGCTTGATGTCAATATCTGTATATACGTTCTATTTTTACATTCTTTGCGACCAGTAAGTTCATTAGCTAGACGTTATGCTGTACAGGACAAATGAGGAGTATTAGAAATATGCATCAACAGTAATCTATATTTGTATGATTGTATCTATGGAATCTATACAACGTTCTTTTCATTTTCACCCCTCTTCCCATCACCATATTCTTATTTTCAATCTACTTGTTCTTTTCCGTTTTCTTCTTTTTACGGTCTTCGTGTTATTATTGTTGCTGTCATTTTATTTATTTACTTTAATCTCAGGATGCTGCTGTACAGCCTTCTTTGGTAATAGTTTATTTTGGTGGTAATGATTCAATGGGGCCCCATCCATCCGGCCTTGGTCCTCATGTACCGCTTCCTGAATACATTGATAACATGAGAAAAATTGCAACTCATATCCAGGTATTTCAAACAGAAACTTCATATTAACACCAGGCTACTAAATTGTTCCTTAAATGACTCTTGAAAGTGAAATTAGTAGGTAAAAAGATCTGCCTGTCCTTTGTTGAGTGTAACGTAGCTTCTAGCTAACGTACTGACTCGTTGTGAAAAGAAGAAATTCAATCATACTCTTCATTTTCTTATGTTAACTATTGTTGATGCATATACTTACATAAATCCTTAAAAAAACAGAGCCTTTCAGATAAAACGCGCCTTATCTTTTTAACGTGTCCTCCGGTGAACGAGAACAAAGTTCGTGAAAGTCAAAGGTGCTCAACACAATTTAACGCATTGTTTCAATGTTCTTGTTTATTTTGTGTTTTCATTCTAAGATGGTTCATTTGTGACTAAGCAGCAAGTTCCTCAGTGAACTGGTGAGAACAAACGAATTGTGCAAAGCTTATGCCCAAGCATGTATAAAGTTGTGCCAAGAAATGGGTATAAAAGTTATTGATCTTTTTTCTGCCATCCAAAAAAGAGACGATTGGACAAACGTTTGCTTCACGTAAGTTCGTTTCCATTGCCATTTTGAATATTGATTGTAAATTCCATTATCTTGTCTGTAAGATGCTACGAGTTTTAGGAAATCGAAAGACGTTGGAAGTCTTCTTTTCGGTTGTTTTTTTAATAGTTTGAAGCGATTTTATTTCCCCATTTTGCAGAGATGGGATTCATTTGTCAGCTGAAGGAAGCAAAGTATTGGTAGAGGAGATTATGAAAGTTCTCAAAGAAGCTGAATGGCAGCCATCTCTGTTCTGGAAGTCCCTTCCAACAGAGTTTGCAGAAGACTCGCCTTACGATCTCATTTCAGCTGATGGCACGAAAACTCTCAACGGGTCCGATTGGATCATTCACCGAGATATTATGTGGGATTAGTCTTCTCATCAACCTCCATTTCGTCGCACCCATCTTACAGAAATCATTTGCTCAACAATGGCAGACATGGAAGAACTGCATAGAAGGCAGAAAGAATGTAAGAGAAGTATGCAGTTCTGATCTTTCATTGTTGCAATTACAAGCAGCAGAAAATTTTAGGATTAAGTGGATAGAATGTGCAACGTGGTATTAGAAATTAGTAATAAGATCTTCATAAACTTCTTAGAAATTAATAATATCATTTGTGCTGCTTTATTTCTGTATTGATCTCCATGCAAAGCCAGTAAGCAAGCTCAGTTGATGTAGTCAAAAGAAGAAATTTTCAGTTTTGTTTTTTGTTTCTTATGTTTATATAGCAAATAGATTTGATTTGAGAGATTTTAATTATGGAAAAGATGAATACTTGTGAAATGTTTGTGTTTCAATCTAATTTAGAACCAAAGGTATGAAGAAGTGCTACTTTATCAAAGTATTTTG

mRNA sequence

AAAAAAGAAGAATTTAAGAATAAATGTGGTGTTTTTAAAAACAAAATTTTAACAAACAGTTTGTAGGGGAAAGGTAGGAATGTGTAGCGGTGGGAGGTAAAGAAATGGCAAAACGCAACAAAATCAGCGGTTTATAAAATGAAGCGAAATGAATGCTTTTAGAGGCAAAAAGCTACAACTGCAAGAAACCCCTTTAAGGCCTTTTCAAATTTCATTTTCAATTTGGTCTTCGTTTCTCTCTCTCTTGAGGTTGTGGTTGCAGCCTTAACTTCATCCGAGCAAGAGGGAGTGAAAATCGGCGACATGGTAGGACCTTCGAGACCTCAGTTTGTTCTTTTTGGATCCTCCATTACTCAGTTCAGCTTCTCCAATGGCGGTTGGGGTGCTATTCTTGCTGATGTTTATGCTCGCAAAGCAGATATTCTTCTACGAGGTTACTTAAGTTGGAATTCTCGGCGAGCGGTACAGGTTCTTGATCAGATTTTTCCAAAGGTATGGTGTCAAAATGAAGATGCTGCTGTACAGCCTTCTTTGGTAATAGTTTATTTTGGTGGTAATGATTCAATGGGGCCCCATCCATCCGGCCTTGGTCCTCATGTACCGCTTCCTGAATACATTGATAACATGAGAAAAATTGCAACTCATATCCAGAGCCTTTCAGATAAAACGCGCCTTATCTTTTTAACGTGTCCTCCGGTGAACGAGAACAAAGTTCGTGAAAGTCAAAGCAAGTTCCTCAGTGAACTGGTGAGAACAAACGAATTGTGCAAAGCTTATGCCCAAGCATGTATAAAGTTGTGCCAAGAAATGGGTATAAAAGTTATTGATCTTTTTTCTGCCATCCAAAAAAGAGACGATTGGACAAACGTTTGCTTCACAGATGGGATTCATTTGTCAGCTGAAGGAAGCAAAGTATTGGTAGAGGAGATTATGAAAGTTCTCAAAGAAGCTGAATGGCAGCCATCTCTGTTCTGGAAGTCCCTTCCAACAGAGTTTGCAGAAGACTCGCCTTACGATCTCATTTCAGCTGATGGCACGAAAACTCTCAACGGGTCCGATTGGATCATTCACCGAGATATTATGTGGGATTAGTCTTCTCATCAACCTCCATTTCGTCGCACCCATCTTACAGAAATCATTTGCTCAACAATGGCAGACATGGAAGAACTGCATAGAAGGCAGAAAGAATGTAAGAGAAGTATGCAGTTCTGATCTTTCATTGTTGCAATTACAAGCAGCAGAAAATTTTAGGATTAAGTGGATAGAATGTGCAACGTGGTATTAGAAATTAGTAATAAGATCTTCATAAACTTCTTAGAAATTAATAATATCATTTGTGCTGCTTTATTTCTGTATTGATCTCCATGCAAAGCCAGTAAGCAAGCTCAGTTGATGTAGTCAAAAGAAGAAATTTTCAGTTTTGTTTTTTGTTTCTTATGTTTATATAGCAAATAGATTTGATTTGAGAGATTTTAATTATGGAAAAGATGAATACTTGTGAAATGTTTGTGTTTCAATCTAATTTAGAACCAAAGGTATGAAGAAGTGCTACTTTATCAAAGTATTTTG

Coding sequence (CDS)

ATGGTAGGACCTTCGAGACCTCAGTTTGTTCTTTTTGGATCCTCCATTACTCAGTTCAGCTTCTCCAATGGCGGTTGGGGTGCTATTCTTGCTGATGTTTATGCTCGCAAAGCAGATATTCTTCTACGAGGTTACTTAAGTTGGAATTCTCGGCGAGCGGTACAGGTTCTTGATCAGATTTTTCCAAAGGTATGGTGTCAAAATGAAGATGCTGCTGTACAGCCTTCTTTGGTAATAGTTTATTTTGGTGGTAATGATTCAATGGGGCCCCATCCATCCGGCCTTGGTCCTCATGTACCGCTTCCTGAATACATTGATAACATGAGAAAAATTGCAACTCATATCCAGAGCCTTTCAGATAAAACGCGCCTTATCTTTTTAACGTGTCCTCCGGTGAACGAGAACAAAGTTCGTGAAAGTCAAAGCAAGTTCCTCAGTGAACTGGTGAGAACAAACGAATTGTGCAAAGCTTATGCCCAAGCATGTATAAAGTTGTGCCAAGAAATGGGTATAAAAGTTATTGATCTTTTTTCTGCCATCCAAAAAAGAGACGATTGGACAAACGTTTGCTTCACAGATGGGATTCATTTGTCAGCTGAAGGAAGCAAAGTATTGGTAGAGGAGATTATGAAAGTTCTCAAAGAAGCTGAATGGCAGCCATCTCTGTTCTGGAAGTCCCTTCCAACAGAGTTTGCAGAAGACTCGCCTTACGATCTCATTTCAGCTGATGGCACGAAAACTCTCAACGGGTCCGATTGGATCATTCACCGAGATATTATGTGGGATTAG

Protein sequence

MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQIFPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSDKTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAIQKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLISADGTKTLNGSDWIIHRDIMWD
Homology
BLAST of Lsi04G009750 vs. ExPASy Swiss-Prot
Match: Q9SRM5 (GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.0e-110
Identity = 181/262 (69.08%), Postives = 222/262 (84.73%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGP+RPQ VLFGSSI Q SF +GGWGAIL++VYARKADI+LRGY  WNS RA++V+DQ+
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSM PH SGLGPHVPL EY+DNM+KIA H+QSLSD
Sbjct: 61  FPK------DAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
            TR+IFL+ PPV+E KVR++QS +LSE++RTN+LCK Y+ AC++LCQE+G++V+DLFS  
Sbjct: 121 FTRIIFLSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTF 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QK DDW  VCFTDGIHLSA+GSK++  EI++V+KEAEW PSL WKS+PTEFA+DSPYDL+
Sbjct: 181 QKADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFADDSPYDLV 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           SADG +T+N S+W    +  WD
Sbjct: 241 SADGKQTVNSSEWTYFWEEQWD 256

BLAST of Lsi04G009750 vs. ExPASy Swiss-Prot
Match: O80443 (GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 3.9e-81
Identity = 142/234 (60.68%), Postives = 183/234 (78.21%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGP RPQ VLFGSSI Q+SF++ GWGA LAD+Y+R ADI+LRGY  WNSR A++VL Q+
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DA +QPSLVIVYFGGNDS  PHPSG GPHVPL E+I+NMRKI  H+ SLSD
Sbjct: 61  FPK------DAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTR+IFLT PP+NE ++       +    R+NELC+ YA+  + LC+E+ +K ID+++AI
Sbjct: 121 KTRVIFLTPPPMNEKQIEIVFGDAIKG--RSNELCRPYAEELLNLCREINVKGIDIWTAI 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAED 235
           Q++DDW N CFTDGIH +A+ S+++V+EI+KVL+ A+W+PSL+WKSLP EF  D
Sbjct: 181 QQQDDWLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFPFD 226

BLAST of Lsi04G009750 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 172.2 bits (435), Expect = 7.9e-42
Identity = 90/238 (37.82%), Postives = 140/238 (58.82%), Query Frame = 0

Query: 6   RPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQIFPKVW 65
           RP+ VLFG SIT  SF +GGWG+ LAD Y+RKAD+++RGY  +N+R A+ +L  IFP   
Sbjct: 2   RPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPL-- 61

Query: 66  CQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSDKTRLI 125
                ++  P    ++FG ND+     +    HVP+ EY DN+RKI  H++  S    ++
Sbjct: 62  ----GSSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIV 121

Query: 126 FLTCPPVNE---NKVRES--QSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 185
            +T PP++E       ES    K + E  RTNE    YAQ C+ L +E+G++ ++L+S +
Sbjct: 122 LITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKM 181

Query: 186 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYD 239
           Q+ +DW     +DG+HL+ EG+ V+ +E+ +V +EA   P    + +P +F   S  D
Sbjct: 182 QETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSP----EEMPFDFPHHSHID 229

BLAST of Lsi04G009750 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.8e-41
Identity = 91/212 (42.92%), Postives = 134/212 (63.21%), Query Frame = 0

Query: 6   RPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQIFPKVW 65
           R +  LFG SIT+ SFS+GGWGA LAD+  RKAD++LRGY  +N+R A++V++++FP   
Sbjct: 3   RRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFP--- 62

Query: 66  CQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSDKTRLI 125
              ED    P+ V V+FG ND+  P       HVPL EY  N+R I + +++   +T +I
Sbjct: 63  VAEEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAII 122

Query: 126 FLTCPPVNEN-KVRESQSKFLSEL-VRTNELCKAYAQACIKLCQEMGIKVIDLFSAIQKR 185
            +T PP++E  ++R    +  + L  RTNE+   YA+ACI + +E  I V DL+S +Q+ 
Sbjct: 123 LITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQI 182

Query: 186 DDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKE 216
            +W   C  DG+HLS  G+KV+ EE+ K LKE
Sbjct: 183 PNWQTECLWDGLHLSRVGNKVVFEEVAKKLKE 211

BLAST of Lsi04G009750 vs. ExPASy Swiss-Prot
Match: Q3SZ16 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.0e-29
Identity = 81/242 (33.47%), Postives = 131/242 (54.13%), Query Frame = 0

Query: 7   PQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQIFPKVWC 66
           P+ +LFG SITQFSF  GGWGA LAD+  RK D+L RG+  +N+R A  +L ++  K   
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGASLADMLVRKCDVLNRGFSGYNTRWAKIILPRLVRK--- 75

Query: 67  QNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLS-DKTRLI 126
                   P  V ++FG NDS          HVPL E++ N+R +  +++S+   + RLI
Sbjct: 76  --GSGLDSPVAVTIFFGANDS-ALKDENPKQHVPLEEFVANLRSMVRYLRSVDVPEGRLI 135

Query: 127 FLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAIQKRDD 186
            +T PP+ E    +   +   +L R N +   YA+AC+++ Q+ G   +DL+S +QK   
Sbjct: 136 LITPPPLCEAAWAQECLQQGCKLNRLNSVVGEYARACLQVAQDCGADALDLWSLMQKDGQ 195

Query: 187 WTNVCFTDGIHLSAEGSKVLVEEIMKVL-KEAEWQPSL--FWKSLPTEFAEDSPYDLISA 245
             +   +DG+HLS +G++ +   +  ++ K+    P L  +W+    + AE  P   +  
Sbjct: 196 DFSSYLSDGLHLSPKGNEFVFSHLWPLIEKKVSSLPFLLPYWR----DIAEARPELSLLG 247

BLAST of Lsi04G009750 vs. ExPASy TrEMBL
Match: A0A1S3CIS5 (GDSL esterase/lipase CPRD49 OS=Cucumis melo OX=3656 GN=LOC103501439 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 244/262 (93.13%), Postives = 253/262 (96.56%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSF+NGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQ+
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFANGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEY+DNMRKIATHIQSLSD
Sbjct: 61  FPK------DAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYVDNMRKIATHIQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVRESQSKFLSELVRTNELCK YAQACIKLCQE+GIKV+DLF+AI
Sbjct: 121 KTRLIFLTCPPVNETKVRESQSKFLSELVRTNELCKVYAQACIKLCQEIGIKVVDLFTAI 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW NVCFTDGIHLSAEGSKV+VEEIMKVLKEAEW+PSLFWKSLPTEFAEDSPYDLI
Sbjct: 181 QKRDDWMNVCFTDGIHLSAEGSKVVVEEIMKVLKEAEWKPSLFWKSLPTEFAEDSPYDLI 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADGTKTLNGSDWIIHRDIMWD
Sbjct: 241 AADGTKTLNGSDWIIHRDIMWD 256

BLAST of Lsi04G009750 vs. ExPASy TrEMBL
Match: A0A0A0KQH5 (SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G202360 PE=4 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 5.5e-139
Identity = 240/262 (91.60%), Postives = 251/262 (95.80%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSF+NGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQ+
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFANGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      D  VQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD
Sbjct: 61  FPK------DTVVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVR SQSKFLSELVRTNELCKAYAQACIKLCQE+G+KV+DLF+AI
Sbjct: 121 KTRLIFLTCPPVNETKVRGSQSKFLSELVRTNELCKAYAQACIKLCQEIGVKVVDLFTAI 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW NVCFTDGIHLSAEGSKV+VEEIMKVLKEAEW+PSL+WKSLPTEFAEDSPYDL+
Sbjct: 181 QKRDDWMNVCFTDGIHLSAEGSKVVVEEIMKVLKEAEWKPSLYWKSLPTEFAEDSPYDLV 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADGTKTLNGSDWIIHRDIMWD
Sbjct: 241 TADGTKTLNGSDWIIHRDIMWD 256

BLAST of Lsi04G009750 vs. ExPASy TrEMBL
Match: A0A6J1GWK0 (GDSL esterase/lipase CPRD49-like OS=Cucurbita moschata OX=3662 GN=LOC111458117 PE=4 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.1e-138
Identity = 239/262 (91.22%), Postives = 249/262 (95.04%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADI LRGYLSWNSRRAVQVLDQ+
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADIFLRGYLSWNSRRAVQVLDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSMGPH SGLGPHVPLPEY+DNMRKIATHIQSLSD
Sbjct: 61  FPK------DAAVQPSLVIVYFGGNDSMGPHASGLGPHVPLPEYVDNMRKIATHIQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVRES SKFLSELVRTNELCKAYAQAC+KLCQEMG+KV+DLFSA 
Sbjct: 121 KTRLIFLTCPPVNETKVRESSSKFLSELVRTNELCKAYAQACVKLCQEMGVKVVDLFSAF 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW NVC TDGIHLSAEGSKV+VEEIMKVLKEAEW+PSLFWKSLPTEFAEDSPYDL+
Sbjct: 181 QKRDDWMNVCLTDGIHLSAEGSKVVVEEIMKVLKEAEWKPSLFWKSLPTEFAEDSPYDLV 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADG+KTLNGSDWIIHRDIMWD
Sbjct: 241 AADGSKTLNGSDWIIHRDIMWD 256

BLAST of Lsi04G009750 vs. ExPASy TrEMBL
Match: A0A6J1JQZ8 (GDSL esterase/lipase CPRD49-like OS=Cucurbita maxima OX=3661 GN=LOC111488114 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 6.8e-137
Identity = 236/262 (90.08%), Postives = 247/262 (94.27%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADI LRGYLSWNSRRAVQVLDQ+
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADIFLRGYLSWNSRRAVQVLDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSMGPH SGLGPHVPLPEY+DNMRKIATHIQ LSD
Sbjct: 61  FPK------DAAVQPSLVIVYFGGNDSMGPHASGLGPHVPLPEYVDNMRKIATHIQGLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVRES SKFLSELVRTNELCK YAQAC+KLCQEMG+KV+DLFSA 
Sbjct: 121 KTRLIFLTCPPVNETKVRESSSKFLSELVRTNELCKGYAQACVKLCQEMGVKVVDLFSAF 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW NVC TDGIHLSAEGSKV+VEEIMKVLKEAEW+PSLFWKSLPTEFAEDSPYDL+
Sbjct: 181 QKRDDWMNVCLTDGIHLSAEGSKVVVEEIMKVLKEAEWKPSLFWKSLPTEFAEDSPYDLV 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +A+G+KTLNGSDWIIHRDIMWD
Sbjct: 241 AANGSKTLNGSDWIIHRDIMWD 256

BLAST of Lsi04G009750 vs. ExPASy TrEMBL
Match: A0A6J1FTH8 (GDSL esterase/lipase CPRD49-like OS=Cucurbita moschata OX=3662 GN=LOC111447982 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 8.9e-137
Identity = 235/262 (89.69%), Postives = 249/262 (95.04%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSFSNGGWGA LADVYARKADILLRGYLSWNSRRA+QVLDQI
Sbjct: 11  MVGPSRPQFVLFGSSITQFSFSNGGWGATLADVYARKADILLRGYLSWNSRRAIQVLDQI 70

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSMGPH SGLGPHVPLPEY+DNMRKIATHIQSLSD
Sbjct: 71  FPK------DAAVQPSLVIVYFGGNDSMGPHASGLGPHVPLPEYVDNMRKIATHIQSLSD 130

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           +TRLIFLTCPPVNE KVRES SKFLSELVRTNELCKAY+QACIKLCQEM IKV+DLF+AI
Sbjct: 131 ETRLIFLTCPPVNETKVRESHSKFLSELVRTNELCKAYSQACIKLCQEMDIKVVDLFTAI 190

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           Q+RDDW NVCFTDGIHLSAEGSKV+VEE+MK+LKEAEW+PSLFWKSLPTEFAEDSPYDL+
Sbjct: 191 QQRDDWMNVCFTDGIHLSAEGSKVVVEEVMKILKEAEWKPSLFWKSLPTEFAEDSPYDLV 250

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADGTKTLNGSDW+IHRD MWD
Sbjct: 251 TADGTKTLNGSDWVIHRDTMWD 266

BLAST of Lsi04G009750 vs. NCBI nr
Match: XP_008463235.1 (PREDICTED: GDSL esterase/lipase CPRD49 [Cucumis melo])

HSP 1 Score: 509.2 bits (1310), Expect = 2.1e-140
Identity = 244/262 (93.13%), Postives = 253/262 (96.56%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSF+NGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQ+
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFANGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEY+DNMRKIATHIQSLSD
Sbjct: 61  FPK------DAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYVDNMRKIATHIQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVRESQSKFLSELVRTNELCK YAQACIKLCQE+GIKV+DLF+AI
Sbjct: 121 KTRLIFLTCPPVNETKVRESQSKFLSELVRTNELCKVYAQACIKLCQEIGIKVVDLFTAI 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW NVCFTDGIHLSAEGSKV+VEEIMKVLKEAEW+PSLFWKSLPTEFAEDSPYDLI
Sbjct: 181 QKRDDWMNVCFTDGIHLSAEGSKVVVEEIMKVLKEAEWKPSLFWKSLPTEFAEDSPYDLI 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADGTKTLNGSDWIIHRDIMWD
Sbjct: 241 AADGTKTLNGSDWIIHRDIMWD 256

BLAST of Lsi04G009750 vs. NCBI nr
Match: XP_004149235.1 (GDSL esterase/lipase CPRD49 [Cucumis sativus] >KGN50652.1 hypothetical protein Csa_005811 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 1.1e-138
Identity = 240/262 (91.60%), Postives = 251/262 (95.80%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSF+NGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQ+
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFANGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      D  VQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD
Sbjct: 61  FPK------DTVVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVR SQSKFLSELVRTNELCKAYAQACIKLCQE+G+KV+DLF+AI
Sbjct: 121 KTRLIFLTCPPVNETKVRGSQSKFLSELVRTNELCKAYAQACIKLCQEIGVKVVDLFTAI 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW NVCFTDGIHLSAEGSKV+VEEIMKVLKEAEW+PSL+WKSLPTEFAEDSPYDL+
Sbjct: 181 QKRDDWMNVCFTDGIHLSAEGSKVVVEEIMKVLKEAEWKPSLYWKSLPTEFAEDSPYDLV 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADGTKTLNGSDWIIHRDIMWD
Sbjct: 241 TADGTKTLNGSDWIIHRDIMWD 256

BLAST of Lsi04G009750 vs. NCBI nr
Match: XP_038891485.1 (GDSL esterase/lipase CPRD49 [Benincasa hispida])

HSP 1 Score: 502.7 bits (1293), Expect = 2.0e-138
Identity = 239/262 (91.22%), Postives = 251/262 (95.80%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRA+QVLDQI
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRALQVLDQI 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAA+QPSLVIVYFGGNDSMGPHPSGLGPHVPLPEY+DNMRKIATHIQSLSD
Sbjct: 61  FPK------DAAIQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYVDNMRKIATHIQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVRE QSKFLSELVRTNELCKAYAQACIKLCQEMG+KV+DLF+AI
Sbjct: 121 KTRLIFLTCPPVNETKVREGQSKFLSELVRTNELCKAYAQACIKLCQEMGVKVVDLFTAI 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW N+CFTDGIHLSAEGSKV+VEEIMK+LKEAEW+PSLFWKSLPTEFAEDSPYDLI
Sbjct: 181 QKRDDWMNLCFTDGIHLSAEGSKVVVEEIMKILKEAEWKPSLFWKSLPTEFAEDSPYDLI 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADG KTLNGSDWIIHRD +WD
Sbjct: 241 AADGMKTLNGSDWIIHRDSIWD 256

BLAST of Lsi04G009750 vs. NCBI nr
Match: XP_022956352.1 (GDSL esterase/lipase CPRD49-like [Cucurbita moschata] >XP_022956353.1 GDSL esterase/lipase CPRD49-like [Cucurbita moschata] >KAG6600930.1 GDSL esterase/lipase CPRD49, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031566.1 GDSL esterase/lipase CPRD49 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501.5 bits (1290), Expect = 4.4e-138
Identity = 239/262 (91.22%), Postives = 249/262 (95.04%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADI LRGYLSWNSRRAVQVLDQ+
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADIFLRGYLSWNSRRAVQVLDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSMGPH SGLGPHVPLPEY+DNMRKIATHIQSLSD
Sbjct: 61  FPK------DAAVQPSLVIVYFGGNDSMGPHASGLGPHVPLPEYVDNMRKIATHIQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVRES SKFLSELVRTNELCKAYAQAC+KLCQEMG+KV+DLFSA 
Sbjct: 121 KTRLIFLTCPPVNETKVRESSSKFLSELVRTNELCKAYAQACVKLCQEMGVKVVDLFSAF 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW NVC TDGIHLSAEGSKV+VEEIMKVLKEAEW+PSLFWKSLPTEFAEDSPYDL+
Sbjct: 181 QKRDDWMNVCLTDGIHLSAEGSKVVVEEIMKVLKEAEWKPSLFWKSLPTEFAEDSPYDLV 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADG+KTLNGSDWIIHRDIMWD
Sbjct: 241 AADGSKTLNGSDWIIHRDIMWD 256

BLAST of Lsi04G009750 vs. NCBI nr
Match: XP_023523632.1 (GDSL esterase/lipase CPRD49-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500.7 bits (1288), Expect = 7.4e-138
Identity = 239/262 (91.22%), Postives = 249/262 (95.04%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGPSRPQFVLFGSSITQFSFSNGGWGAIL+DVYARKADI LRGYLSWNSRRAVQVLDQ+
Sbjct: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILSDVYARKADIFLRGYLSWNSRRAVQVLDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSMGPH SGLGPHVPLPEYIDNMRKIATHIQSLSD
Sbjct: 61  FPK------DAAVQPSLVIVYFGGNDSMGPHASGLGPHVPLPEYIDNMRKIATHIQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTRLIFLTCPPVNE KVRES SKFLSELVRTNELCKAYAQAC+KLCQEMG+KV+DLFSA 
Sbjct: 121 KTRLIFLTCPPVNETKVRESSSKFLSELVRTNELCKAYAQACVKLCQEMGVKVVDLFSAF 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QKRDDW NVC TDGIHLSAEGSKV+VEEIMKVLKEAEW+PSLFWKSLPTEFAEDSPYDL+
Sbjct: 181 QKRDDWMNVCLTDGIHLSAEGSKVVVEEIMKVLKEAEWKPSLFWKSLPTEFAEDSPYDLV 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           +ADG+KTLNGSDWIIHRDIMWD
Sbjct: 241 AADGSKTLNGSDWIIHRDIMWD 256

BLAST of Lsi04G009750 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 401.0 bits (1029), Expect = 7.5e-112
Identity = 181/262 (69.08%), Postives = 222/262 (84.73%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGP+RPQ VLFGSSI Q SF +GGWGAIL++VYARKADI+LRGY  WNS RA++V+DQ+
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DAAVQPSLVIVYFGGNDSM PH SGLGPHVPL EY+DNM+KIA H+QSLSD
Sbjct: 61  FPK------DAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
            TR+IFL+ PPV+E KVR++QS +LSE++RTN+LCK Y+ AC++LCQE+G++V+DLFS  
Sbjct: 121 FTRIIFLSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTF 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYDLI 240
           QK DDW  VCFTDGIHLSA+GSK++  EI++V+KEAEW PSL WKS+PTEFA+DSPYDL+
Sbjct: 181 QKADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFADDSPYDLV 240

Query: 241 SADGTKTLNGSDWIIHRDIMWD 263
           SADG +T+N S+W    +  WD
Sbjct: 241 SADGKQTVNSSEWTYFWEEQWD 256

BLAST of Lsi04G009750 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 2.8e-82
Identity = 142/234 (60.68%), Postives = 183/234 (78.21%), Query Frame = 0

Query: 1   MVGPSRPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQI 60
           MVGP RPQ VLFGSSI Q+SF++ GWGA LAD+Y+R ADI+LRGY  WNSR A++VL Q+
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 61  FPKVWCQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSD 120
           FPK      DA +QPSLVIVYFGGNDS  PHPSG GPHVPL E+I+NMRKI  H+ SLSD
Sbjct: 61  FPK------DAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSD 120

Query: 121 KTRLIFLTCPPVNENKVRESQSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 180
           KTR+IFLT PP+NE ++       +    R+NELC+ YA+  + LC+E+ +K ID+++AI
Sbjct: 121 KTRVIFLTPPPMNEKQIEIVFGDAIKG--RSNELCRPYAEELLNLCREINVKGIDIWTAI 180

Query: 181 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAED 235
           Q++DDW N CFTDGIH +A+ S+++V+EI+KVL+ A+W+PSL+WKSLP EF  D
Sbjct: 181 QQQDDWLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFPFD 226

BLAST of Lsi04G009750 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 5.6e-43
Identity = 90/238 (37.82%), Postives = 140/238 (58.82%), Query Frame = 0

Query: 6   RPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQIFPKVW 65
           RP+ VLFG SIT  SF +GGWG+ LAD Y+RKAD+++RGY  +N+R A+ +L  IFP   
Sbjct: 2   RPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPL-- 61

Query: 66  CQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSDKTRLI 125
                ++  P    ++FG ND+     +    HVP+ EY DN+RKI  H++  S    ++
Sbjct: 62  ----GSSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIV 121

Query: 126 FLTCPPVNE---NKVRES--QSKFLSELVRTNELCKAYAQACIKLCQEMGIKVIDLFSAI 185
            +T PP++E       ES    K + E  RTNE    YAQ C+ L +E+G++ ++L+S +
Sbjct: 122 LITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKM 181

Query: 186 QKRDDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKEAEWQPSLFWKSLPTEFAEDSPYD 239
           Q+ +DW     +DG+HL+ EG+ V+ +E+ +V +EA   P    + +P +F   S  D
Sbjct: 182 QETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSP----EEMPFDFPHHSHID 229

BLAST of Lsi04G009750 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.3e-42
Identity = 91/212 (42.92%), Postives = 134/212 (63.21%), Query Frame = 0

Query: 6   RPQFVLFGSSITQFSFSNGGWGAILADVYARKADILLRGYLSWNSRRAVQVLDQIFPKVW 65
           R +  LFG SIT+ SFS+GGWGA LAD+  RKAD++LRGY  +N+R A++V++++FP   
Sbjct: 3   RRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFP--- 62

Query: 66  CQNEDAAVQPSLVIVYFGGNDSMGPHPSGLGPHVPLPEYIDNMRKIATHIQSLSDKTRLI 125
              ED    P+ V V+FG ND+  P       HVPL EY  N+R I + +++   +T +I
Sbjct: 63  VAEEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAII 122

Query: 126 FLTCPPVNEN-KVRESQSKFLSEL-VRTNELCKAYAQACIKLCQEMGIKVIDLFSAIQKR 185
            +T PP++E  ++R    +  + L  RTNE+   YA+ACI + +E  I V DL+S +Q+ 
Sbjct: 123 LITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQI 182

Query: 186 DDWTNVCFTDGIHLSAEGSKVLVEEIMKVLKE 216
            +W   C  DG+HLS  G+KV+ EE+ K LKE
Sbjct: 183 PNWQTECLWDGLHLSRVGNKVVFEEVAKKLKE 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRM51.0e-11069.08GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1[more]
O804433.9e-8160.68GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2... [more]
Q9FM047.9e-4237.82GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2... [more]
Q6NMR91.8e-4142.92GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2... [more]
Q3SZ162.0e-2933.47Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S3CIS51.0e-14093.13GDSL esterase/lipase CPRD49 OS=Cucumis melo OX=3656 GN=LOC103501439 PE=4 SV=1[more]
A0A0A0KQH55.5e-13991.60SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G202360 ... [more]
A0A6J1GWK02.1e-13891.22GDSL esterase/lipase CPRD49-like OS=Cucurbita moschata OX=3662 GN=LOC111458117 P... [more]
A0A6J1JQZ86.8e-13790.08GDSL esterase/lipase CPRD49-like OS=Cucurbita maxima OX=3661 GN=LOC111488114 PE=... [more]
A0A6J1FTH88.9e-13789.69GDSL esterase/lipase CPRD49-like OS=Cucurbita moschata OX=3662 GN=LOC111447982 P... [more]
Match NameE-valueIdentityDescription
XP_008463235.12.1e-14093.13PREDICTED: GDSL esterase/lipase CPRD49 [Cucumis melo][more]
XP_004149235.11.1e-13891.60GDSL esterase/lipase CPRD49 [Cucumis sativus] >KGN50652.1 hypothetical protein C... [more]
XP_038891485.12.0e-13891.22GDSL esterase/lipase CPRD49 [Benincasa hispida][more]
XP_022956352.14.4e-13891.22GDSL esterase/lipase CPRD49-like [Cucurbita moschata] >XP_022956353.1 GDSL ester... [more]
XP_023523632.17.4e-13891.22GDSL esterase/lipase CPRD49-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G11210.17.5e-11269.08SGNH hydrolase-type esterase superfamily protein [more]
AT2G38180.12.8e-8260.68SGNH hydrolase-type esterase superfamily protein [more]
AT5G62930.15.6e-4337.82SGNH hydrolase-type esterase superfamily protein [more]
AT5G45920.11.3e-4242.92SGNH hydrolase-type esterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 5..241
e-value: 2.0E-62
score: 212.6
IPR013830SGNH hydrolase-type esterase domainPFAMPF13472Lipase_GDSL_2coord: 11..203
e-value: 6.9E-22
score: 78.7
IPR045136Isoamyl acetate-hydrolyzing esterase Iah1-likePANTHERPTHR14209ISOAMYL ACETATE-HYDROLYZING ESTERASE 1coord: 1..259
NoneNo IPR availablePANTHERPTHR14209:SF28GDSL ESTERASE/LIPASE CPRD49-LIKEcoord: 1..259
NoneNo IPR availableCDDcd01838Isoamyl_acetate_hydrolase_likecoord: 8..213
e-value: 4.7006E-91
score: 265.655
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 4..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G009750.1Lsi04G009750.1mRNA