Lsi01G016180.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G016180.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	protein FATTY ACID EXPORT 3, chloroplastic
Location	chr01: 14554740 .. 14556933 (+)
Sequence length	1118
RNA-Seq Expression	Lsi01G016180.1
Synteny	Lsi01G016180.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAACTTTGACGAAGGAACCACCAAAAAACATTTGATTGATAGGTACATGATTCTTCCTACCAAACCCAAAACCCAAAACGCCAAAAAGATCAAAACTTTCTCATTGTATTTCACACAAAATCTCTCCTTCACCTCTCTTCTCTCTCCATCTCCAACCCCCATTTCTATAAAACCCTAATCCTTTCCTCTTCTGAGCCTCGAATCCCTCGCCATATCCCTTCATCCCTTACCCCTTTCCCTTTCTCTCTCTTTCTCTGCTTCAATTTCTGCTAATGGAGTCGCTTTTGGTTCTCAATTCCACCCCTTGCTCCGGCGACTCGTCCCCTTTGAAGCTCAACAAGTTTGCTGCTGTTCCTCTCCGCTCCCGCCCCTCTTCGTCTCTGCGGATCGATCCCTTGATGGGTTTTGGTGCTTGTAAGGTTTCCTTTGTCGCTAACACTTCTCAGCGTAATGGGTTTCTCTCGCCGTATCGAAAGTGTTCGTTGGGTCGACGGGTTCTGGCGTTTGCCGCGTCGCATGAAGAGTCGGTATGTCGAATTCTGTTCACTTCTGTCACTGCGTTGAATTGTGGGTGCTGATTGGGATAGGGATTAGGGTTAAGCATTCTGTAGAATTCAGGATAATTGTTTATCGGCTTGAGAGTGTGGGATTTTGTTTCTCTGATTATGACTTGGGTGGTTATTGAATGTTACTTGGAGAATGATGAGGTCTGTGGTTTGGTAATTCTTATGTGTATTATGAATTACACTATCGAGCAGTTAAATTAATGTCTTGGGAATTTATTTTTATTTGTTTTCTCTCTATGACATGTGATCGGTAATCTAGCAATAGCTACATTGTCCAGCCAAGCCTTGTTGAATAATGATACTTTTTTTGGCACTCAAGGTTGTTTTTTCTAGGCCAAAATTTTCATGAAAGATATGGTATTTCATTTATTTGAAAGGAGACGTTATCCTTTATCTCTTATGATGATAATAATATGTCTACATACAAGTTTTGAGCTCGATAGATGAAGATTTTTATATGGTGTATTCTTTCATTTCTCTTGACGAAAGCTCGGTTTCTTACCAGAAATAATAATTGTCAATAATTGCCTGACTGGTTTTTGATATTCAGGTTTTAGTATCACGTTAGCTGAAAATGCTGGGTGCTTATTTTAGTTATAAAATTGTGATCTCATGCTAAACGTGGTTACTGAATTTTCAAGGTGGTATAGTTGGTTATATGCATTGAATGTAGATTGATAGGCCCTTTTGTTTTCCTATTTGTATAACTAGCATTCAGAAACTCAAGGGGAGAAAGATGGAAAAGATTTCAATTTTGATGTGGAAAAGGCAGAAGAGTTGTGGAAAAATGCTTTAGATTCCTTCAAAGAACAAGCTCTGAAGATGAAAGCTATTTCAAAGGATGCGTATGATGAATATTCTGAGAAAGCATTAGTTGCTCTGAATGAAACTTCAAAGCTTTTGAAAATTCAAGCTGATAAGGCAAAGGATGATTTGGCTTTAATAGTGCAAGAATTCAGTGAAGAAGGCCAAGAATATATTGCCACTGTCGCTGAGAATTGTCCTGAAGAAGTCAAGGAGATTGTGGAAACATTTACTTCTCCAACTGATGACTTAACTGATATTTCAAGAGTTCATGAATTCTATTATGGGATACCATATGGTATGTATTACCAAACTTTATGTGAGGTTTGAATGGAGGAGGGTCAGCTTTGCTTTAGTACAACCATTTATTTCTGCTACTACTTGAGTACCCATCCAAGTCAAATTTGAATTTGGAGGGAAAGAAACCTTCAGATAGGATAATTGGAAGGAATTTAATATCTGTATATCCTGCAACATTGGCCTTTCAATATTCTATCTATGACTCATTTAGTGGATATTGTTTTTGCGTCAATTTATCTAAGTCCTGTGGGCTTATAAATCTCCTGCTGACCCTTTTTTTATTATTTATTTTTTTCCTTACTTTTTATTACTGATGATCTACAGGACTGGTCCTTTCTTTTGGTGGTTTTCTTTCCTTCATGCTAACGGGAAGTCTTGCTGCAATTAGATTTGGAGTGATACTTGGTGGTGCTCTGTTGGCCTTGAGTATTTTAAGCTTGCGGGCATACAAAAGAGGACAATCACTCCCACTTGCCCTGAAGGGGCAGTCTGGTCAGTCTGCCATTAATTCAATAACATAA

mRNA sequence

AAAAAACTTTGACGAAGGAACCACCAAAAAACATTTGATTGATAGGTACATGATTCTTCCTACCAAACCCAAAACCCAAAACGCCAAAAAGATCAAAACTTTCTCATTGTATTTCACACAAAATCTCTCCTTCACCTCTCTTCTCTCTCCATCTCCAACCCCCATTTCTATAAAACCCTAATCCTTTCCTCTTCTGAGCCTCGAATCCCTCGCCATATCCCTTCATCCCTTACCCCTTTCCCTTTCTCTCTCTTTCTCTGCTTCAATTTCTGCTAATGGAGTCGCTTTTGGTTCTCAATTCCACCCCTTGCTCCGGCGACTCGTCCCCTTTGAAGCTCAACAAGTTTGCTGCTGTTCCTCTCCGCTCCCGCCCCTCTTCGTCTCTGCGGATCGATCCCTTGATGGGTTTTGGTGCTTGTAAGGTTTCCTTTGTCGCTAACACTTCTCAGCGTAATGGGTTTCTCTCGCCGTATCGAAAGTGTTCGTTGGGTCGACGGGTTCTGGCGTTTGCCGCGTCGCATGAAGAGTCGCATTCAGAAACTCAAGGGGAGAAAGATGGAAAAGATTTCAATTTTGATGTGGAAAAGGCAGAAGAGTTGTGGAAAAATGCTTTAGATTCCTTCAAAGAACAAGCTCTGAAGATGAAAGCTATTTCAAAGGATGCGTATGATGAATATTCTGAGAAAGCATTAGTTGCTCTGAATGAAACTTCAAAGCTTTTGAAAATTCAAGCTGATAAGGCAAAGGATGATTTGGCTTTAATAGTGCAAGAATTCAGTGAAGAAGGCCAAGAATATATTGCCACTGTCGCTGAGAATTGTCCTGAAGAAGTCAAGGAGATTGTGGAAACATTTACTTCTCCAACTGATGACTTAACTGATATTTCAAGAGTTCATGAATTCTATTATGGGATACCATATGGACTGGTCCTTTCTTTTGGTGGTTTTCTTTCCTTCATGCTAACGGGAAGTCTTGCTGCAATTAGATTTGGAGTGATACTTGGTGGTGCTCTGTTGGCCTTGAGTATTTTAAGCTTGCGGGCATACAAAAGAGGACAATCACTCCCACTTGCCCTGAAGGGGCAGTCTGGTCAGTCTGCCATTAATTCAATAACATAA

Coding sequence (CDS)

ATGGAGTCGCTTTTGGTTCTCAATTCCACCCCTTGCTCCGGCGACTCGTCCCCTTTGAAGCTCAACAAGTTTGCTGCTGTTCCTCTCCGCTCCCGCCCCTCTTCGTCTCTGCGGATCGATCCCTTGATGGGTTTTGGTGCTTGTAAGGTTTCCTTTGTCGCTAACACTTCTCAGCGTAATGGGTTTCTCTCGCCGTATCGAAAGTGTTCGTTGGGTCGACGGGTTCTGGCGTTTGCCGCGTCGCATGAAGAGTCGCATTCAGAAACTCAAGGGGAGAAAGATGGAAAAGATTTCAATTTTGATGTGGAAAAGGCAGAAGAGTTGTGGAAAAATGCTTTAGATTCCTTCAAAGAACAAGCTCTGAAGATGAAAGCTATTTCAAAGGATGCGTATGATGAATATTCTGAGAAAGCATTAGTTGCTCTGAATGAAACTTCAAAGCTTTTGAAAATTCAAGCTGATAAGGCAAAGGATGATTTGGCTTTAATAGTGCAAGAATTCAGTGAAGAAGGCCAAGAATATATTGCCACTGTCGCTGAGAATTGTCCTGAAGAAGTCAAGGAGATTGTGGAAACATTTACTTCTCCAACTGATGACTTAACTGATATTTCAAGAGTTCATGAATTCTATTATGGGATACCATATGGACTGGTCCTTTCTTTTGGTGGTTTTCTTTCCTTCATGCTAACGGGAAGTCTTGCTGCAATTAGATTTGGAGTGATACTTGGTGGTGCTCTGTTGGCCTTGAGTATTTTAAGCTTGCGGGCATACAAAAGAGGACAATCACTCCCACTTGCCCTGAAGGGGCAGTCTGGTCAGTCTGCCATTAATTCAATAACATAA

Protein sequence

MESLLVLNSTPCSGDSSPLKLNKFAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQRNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKEQALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATVAENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRFGVILGGALLALSILSLRAYKRGQSLPLALKGQSGQSAINSIT

Homology

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BLAST of Lsi01G016180.1 vs. ExPASy Swiss-Prot
Match: Q9ZVH7 (Protein FATTY ACID EXPORT 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAX3 PE=2 SV=2)

HSP 1 Score: 191.8 bits (486), Expect = 1.0e-47
Identity = 111/219 (50.68%), Postives = 155/219 (70.78%), Query Frame = 0


Query: 59  RNGFLSPYRKCSLGRRVLAFAASHEES-HSETQGEKDGKDFNFDVEKAEELWKNALDSFK 118
           R GF SP  +  L R V+AFAASHE+S  S  +  K+  D + + + ++E WK  L+SFK
Sbjct: 57  RFGFTSP--EVLLNRSVVAFAASHEDSGESGVEVGKEKSDIDVEDDTSKEAWKQTLESFK 116

Query: 119 EQALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIAT 178
           EQ  KM+++S +AY   S+KA+  L ETS+ L+IQA+KAK++L    +  SEEG+EYI  
Sbjct: 117 EQVSKMQSVSSEAYSVNSQKAMTVLKETSEQLRIQAEKAKEELGTKAKVVSEEGREYILK 176

Query: 179 VAENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIR 238
            AE  P +VKEIVE F S T+DL ++SR ++F+ GIPYGL+L  GGF++FM++GS+ AIR
Sbjct: 177 AAEESPSDVKEIVEAFAS-TEDLKNVSRANDFHVGIPYGLLLLVGGFINFMVSGSIPAIR 236

Query: 239 FGVILGGALLALSILSLRAYKRGQSLPLALKGQSGQSAI 277
           FGVILGGAL ALS+ SL+++++G+S    LKGQ    AI
Sbjct: 237 FGVILGGALFALSLASLKSHRKGESSTKFLKGQMAIVAI 272

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BLAST of Lsi01G016180.1 vs. ExPASy Swiss-Prot
Match: Q93V66 (Protein FATTY ACID EXPORT 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAX1 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.6e-06
Identity = 46/162 (28.40%), Postives = 82/162 (50.62%), Query Frame = 0


Query: 115 SFKEQALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEY 174
           SF    ++ ++    + D  S +   +L+ T+++ K   +K     + +         + 
Sbjct: 24  SFPCPMIRPQSFVVKSVDGNSSETPASLSYTAEVSKPVVEKTSKPYSTV---------DE 83

Query: 175 IATVAENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLA 234
            AT  E+  E V+E V T           +++H+F +GIPYG ++  GG L F  + +L 
Sbjct: 84  TATNKESITEPVEEDVAT------QPIRAAKIHDFCFGIPYGGLVVSGGLLGFAFSRNLT 143

Query: 235 AIRFGVILGGALLALSILSLRAYKRGQSLPLALKGQSGQSAI 277
           ++  GV+ GG LLALS LSL+ ++ G+S    + GQ+  SA+
Sbjct: 144 SLSTGVLYGGGLLALSTLSLKIWREGKSSFPYILGQAVLSAV 170

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BLAST of Lsi01G016180.1 vs. ExPASy TrEMBL
Match: A0A0A0L2W3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G334130 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.2e-124
Identity = 238/273 (87.18%), Postives = 255/273 (93.41%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNK--FAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQ 60
           MESLLVLNSTPC+GD+SPL L+K   AAVPLRSRPSSSLR+DPLMGFGACKVSFVANT Q
Sbjct: 1   MESLLVLNSTPCAGDASPLNLHKPAAAAVPLRSRPSSSLRLDPLMGFGACKVSFVANTFQ 60

Query: 61  RNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKE 120
           RNGFLSPYRKC+L RRVLAFAASHEESHSETQG+ +GKD  FD EKA+ELWKNALDSFKE
Sbjct: 61  RNGFLSPYRKCTLSRRVLAFAASHEESHSETQGKNEGKDLEFDAEKAQELWKNALDSFKE 120

Query: 121 QALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATV 180
           QA+KMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEE +EYIAT 
Sbjct: 121 QAVKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEESKEYIATA 180

Query: 181 AENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRF 240
            E  PEE+KEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGF+SFMLTGSLAAIRF
Sbjct: 181 TERYPEEIKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFVSFMLTGSLAAIRF 240

Query: 241 GVILGGALLALSILSLRAYKRGQSLPLALKGQS 272
           GVILGG LL LSILSL++YKRGQSLPLALKGQ+
Sbjct: 241 GVILGGGLLVLSILSLQSYKRGQSLPLALKGQA 273

[Close]

BLAST of Lsi01G016180.1 vs. ExPASy TrEMBL
Match: A0A5D3CEP9 (Protein FATTY ACID EXPORT 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00450 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 5.6e-121
Identity = 237/275 (86.18%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNK--FAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQ 60
           MESLLVLNSTPC   +SPLK +K   AAVPLRSRPSSSLR+DPLMGFGACKVSFVANT Q
Sbjct: 1   MESLLVLNSTPC---ASPLKPHKPIAAAVPLRSRPSSSLRLDPLMGFGACKVSFVANTFQ 60

Query: 61  RNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKE 120
           RNGFLS YRKC+L RRVLAFAASHEESHSETQG+K+GKD +FD EKA+ELWKNALDSFKE
Sbjct: 61  RNGFLSSYRKCTLSRRVLAFAASHEESHSETQGKKEGKDLDFDAEKAQELWKNALDSFKE 120

Query: 121 QALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATV 180
           QA+KMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEE +EYI + 
Sbjct: 121 QAVKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEESKEYITSA 180

Query: 181 AENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRF 240
            EN PEE+KEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGF+SFMLTGSLAAIRF
Sbjct: 181 TENYPEEIKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFVSFMLTGSLAAIRF 240

Query: 241 GVILGGALLALSILSLRAYKRGQSLPLALKGQSGQ 274
           GVILGGALL LSILSL++YKRGQSLPLALKGQ+ Q
Sbjct: 241 GVILGGALLVLSILSLQSYKRGQSLPLALKGQAVQ 272

[Close]

BLAST of Lsi01G016180.1 vs. ExPASy TrEMBL
Match: A0A1S3CIV0 (protein FATTY ACID EXPORT 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501466 PE=3 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 9.5e-121
Identity = 236/273 (86.45%), Postives = 254/273 (93.04%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNK--FAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQ 60
           MESLLVLNSTPC   +SPLK +K   AAVPLRSRPSSSLR+DPLMGFGACKVSFVANT Q
Sbjct: 1   MESLLVLNSTPC---ASPLKPHKPIAAAVPLRSRPSSSLRLDPLMGFGACKVSFVANTFQ 60

Query: 61  RNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKE 120
           RNGFLS YRKC+L RRVLAFAASHEESHSETQG+K+GKD +FD EKA+ELWKNALDSFKE
Sbjct: 61  RNGFLSSYRKCTLSRRVLAFAASHEESHSETQGKKEGKDLDFDAEKAQELWKNALDSFKE 120

Query: 121 QALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATV 180
           QA+KMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEE +EYI + 
Sbjct: 121 QAVKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEESKEYITSA 180

Query: 181 AENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRF 240
            EN PEE+KEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGF+SFMLTGSLAAIRF
Sbjct: 181 TENYPEEIKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFVSFMLTGSLAAIRF 240

Query: 241 GVILGGALLALSILSLRAYKRGQSLPLALKGQS 272
           GVILGGALL LSILSL++YKRGQSLPLALKGQ+
Sbjct: 241 GVILGGALLVLSILSLQSYKRGQSLPLALKGQA 270

[Close]

BLAST of Lsi01G016180.1 vs. ExPASy TrEMBL
Match: A0A5A7SGY8 (Protein FATTY ACID EXPORT 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1220G00490 PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.6e-120
Identity = 236/275 (85.82%), Postives = 254/275 (92.36%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNK--FAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQ 60
           MESLLVLNSTPC   +SPLK +K   AAVPLRSRPSSSLR+DPLMGFG CKVSFVANT Q
Sbjct: 1   MESLLVLNSTPC---ASPLKPHKPIAAAVPLRSRPSSSLRLDPLMGFGGCKVSFVANTFQ 60

Query: 61  RNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKE 120
           RNGFLS YRKC+L RRVLAFAASHEESHSETQG+K+GKD +FD EKA+ELWKNALDSFKE
Sbjct: 61  RNGFLSSYRKCTLSRRVLAFAASHEESHSETQGKKEGKDLDFDAEKAQELWKNALDSFKE 120

Query: 121 QALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATV 180
           QA+KMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEE +EYI + 
Sbjct: 121 QAVKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEESKEYITSA 180

Query: 181 AENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRF 240
            EN PEE+KEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGF+SFMLTGSLAAIRF
Sbjct: 181 TENYPEEIKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFVSFMLTGSLAAIRF 240

Query: 241 GVILGGALLALSILSLRAYKRGQSLPLALKGQSGQ 274
           GVILGGALL LSILSL++YKRGQSLPLALKGQ+ Q
Sbjct: 241 GVILGGALLVLSILSLQSYKRGQSLPLALKGQAVQ 272

[Close]

BLAST of Lsi01G016180.1 vs. ExPASy TrEMBL
Match: A0A6J1HP56 (protein FATTY ACID EXPORT 3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466053 PE=3 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 5.6e-113
Identity = 219/271 (80.81%), Postives = 242/271 (89.30%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNKFAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQRN 60
           MESLLVLN TPC  DSS LKL   AA P+RSRP SSLR DPL+ +G CKVSFVANT  RN
Sbjct: 1   MESLLVLNPTPCPADSSTLKLKNSAAFPVRSRPCSSLRFDPLISYGGCKVSFVANTLPRN 60

Query: 61  GFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKEQA 120
           GFLSP+R+CSL RRV+AFAASHEESHSE +GEK+ KDFNFD EKA+ELWKNALDSFKEQA
Sbjct: 61  GFLSPHRRCSLNRRVVAFAASHEESHSEIEGEKNEKDFNFDQEKAQELWKNALDSFKEQA 120

Query: 121 LKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATVAE 180
           LKMKAISK+AY EYS KALVALNETSK LKIQADKAK+DLA IVQEFSEE +EYIATVAE
Sbjct: 121 LKMKAISKEAYGEYSVKALVALNETSKHLKIQADKAKEDLASIVQEFSEESKEYIATVAE 180

Query: 181 NCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRFGV 240
           N P+EVKEIVETFTSP DDL DIS+V +FYYGIPYGLVLS GGFLSFM+TGSLAAIRFG+
Sbjct: 181 NYPDEVKEIVETFTSPDDDLRDISKVKDFYYGIPYGLVLSVGGFLSFMVTGSLAAIRFGM 240

Query: 241 ILGGALLALSILSLRAYKRGQSLPLALKGQS 272
           ILGGALLALS++SL++YKRG+S PLALKGQ+
Sbjct: 241 ILGGALLALSVMSLKSYKRGESRPLALKGQA 271

[Close]

BLAST of Lsi01G016180.1 vs. NCBI nr
Match: XP_038883685.1 (protein FATTY ACID EXPORT 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 474.9 bits (1221), Expect = 4.7e-130
Identity = 250/271 (92.25%), Postives = 259/271 (95.57%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNKFAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQRN 60
           MESLLVLN TPCSGDS PLKLN  A +PLRSRPSSSLR+DPLMGFGACKVSFVANT QRN
Sbjct: 1   MESLLVLNCTPCSGDSFPLKLNMSAVLPLRSRPSSSLRVDPLMGFGACKVSFVANTFQRN 60

Query: 61  GFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKEQA 120
           GFLS YRKCSL RRVLAFAASHEESHSETQGEKD KDFNFD EKA+ELWKNALDSFKEQA
Sbjct: 61  GFLSSYRKCSLSRRVLAFAASHEESHSETQGEKDEKDFNFDAEKAQELWKNALDSFKEQA 120

Query: 121 LKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATVAE 180
           LKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEG+EYIATVAE
Sbjct: 121 LKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGKEYIATVAE 180

Query: 181 NCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRFGV 240
           N PEEVKEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGFLSFMLTGSLAAIRFG+
Sbjct: 181 NYPEEVKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFLSFMLTGSLAAIRFGM 240

Query: 241 ILGGALLALSILSLRAYKRGQSLPLALKGQS 272
           ILGGALLALSILSL+AYKRGQSLPLALKGQ+
Sbjct: 241 ILGGALLALSILSLQAYKRGQSLPLALKGQA 271

[Close]

BLAST of Lsi01G016180.1 vs. NCBI nr
Match: XP_004142682.1 (protein FATTY ACID EXPORT 3, chloroplastic [Cucumis sativus] >KGN54451.1 hypothetical protein Csa_011941 [Cucumis sativus])

HSP 1 Score: 454.5 bits (1168), Expect = 6.5e-124
Identity = 238/273 (87.18%), Postives = 255/273 (93.41%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNK--FAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQ 60
           MESLLVLNSTPC+GD+SPL L+K   AAVPLRSRPSSSLR+DPLMGFGACKVSFVANT Q
Sbjct: 1   MESLLVLNSTPCAGDASPLNLHKPAAAAVPLRSRPSSSLRLDPLMGFGACKVSFVANTFQ 60

Query: 61  RNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKE 120
           RNGFLSPYRKC+L RRVLAFAASHEESHSETQG+ +GKD  FD EKA+ELWKNALDSFKE
Sbjct: 61  RNGFLSPYRKCTLSRRVLAFAASHEESHSETQGKNEGKDLEFDAEKAQELWKNALDSFKE 120

Query: 121 QALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATV 180
           QA+KMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEE +EYIAT 
Sbjct: 121 QAVKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEESKEYIATA 180

Query: 181 AENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRF 240
            E  PEE+KEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGF+SFMLTGSLAAIRF
Sbjct: 181 TERYPEEIKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFVSFMLTGSLAAIRF 240

Query: 241 GVILGGALLALSILSLRAYKRGQSLPLALKGQS 272
           GVILGG LL LSILSL++YKRGQSLPLALKGQ+
Sbjct: 241 GVILGGGLLVLSILSLQSYKRGQSLPLALKGQA 273

[Close]

BLAST of Lsi01G016180.1 vs. NCBI nr
Match: TYK09762.1 (protein FATTY ACID EXPORT 3 [Cucumis melo var. makuwa])

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-120
Identity = 237/275 (86.18%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNK--FAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQ 60
           MESLLVLNSTPC   +SPLK +K   AAVPLRSRPSSSLR+DPLMGFGACKVSFVANT Q
Sbjct: 1   MESLLVLNSTPC---ASPLKPHKPIAAAVPLRSRPSSSLRLDPLMGFGACKVSFVANTFQ 60

Query: 61  RNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKE 120
           RNGFLS YRKC+L RRVLAFAASHEESHSETQG+K+GKD +FD EKA+ELWKNALDSFKE
Sbjct: 61  RNGFLSSYRKCTLSRRVLAFAASHEESHSETQGKKEGKDLDFDAEKAQELWKNALDSFKE 120

Query: 121 QALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATV 180
           QA+KMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEE +EYI + 
Sbjct: 121 QAVKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEESKEYITSA 180

Query: 181 AENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRF 240
            EN PEE+KEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGF+SFMLTGSLAAIRF
Sbjct: 181 TENYPEEIKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFVSFMLTGSLAAIRF 240

Query: 241 GVILGGALLALSILSLRAYKRGQSLPLALKGQSGQ 274
           GVILGGALL LSILSL++YKRGQSLPLALKGQ+ Q
Sbjct: 241 GVILGGALLVLSILSLQSYKRGQSLPLALKGQAVQ 272

[Close]

BLAST of Lsi01G016180.1 vs. NCBI nr
Match: XP_008463262.1 (PREDICTED: protein FATTY ACID EXPORT 3, chloroplastic [Cucumis melo])

HSP 1 Score: 443.0 bits (1138), Expect = 2.0e-120
Identity = 236/273 (86.45%), Postives = 254/273 (93.04%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNK--FAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQ 60
           MESLLVLNSTPC   +SPLK +K   AAVPLRSRPSSSLR+DPLMGFGACKVSFVANT Q
Sbjct: 1   MESLLVLNSTPC---ASPLKPHKPIAAAVPLRSRPSSSLRLDPLMGFGACKVSFVANTFQ 60

Query: 61  RNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKE 120
           RNGFLS YRKC+L RRVLAFAASHEESHSETQG+K+GKD +FD EKA+ELWKNALDSFKE
Sbjct: 61  RNGFLSSYRKCTLSRRVLAFAASHEESHSETQGKKEGKDLDFDAEKAQELWKNALDSFKE 120

Query: 121 QALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATV 180
           QA+KMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEE +EYI + 
Sbjct: 121 QAVKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEESKEYITSA 180

Query: 181 AENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRF 240
            EN PEE+KEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGF+SFMLTGSLAAIRF
Sbjct: 181 TENYPEEIKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFVSFMLTGSLAAIRF 240

Query: 241 GVILGGALLALSILSLRAYKRGQSLPLALKGQS 272
           GVILGGALL LSILSL++YKRGQSLPLALKGQ+
Sbjct: 241 GVILGGALLVLSILSLQSYKRGQSLPLALKGQA 270

[Close]

BLAST of Lsi01G016180.1 vs. NCBI nr
Match: KAA0025410.1 (protein FATTY ACID EXPORT 3 [Cucumis melo var. makuwa])

HSP 1 Score: 442.2 bits (1136), Expect = 3.3e-120
Identity = 236/275 (85.82%), Postives = 254/275 (92.36%), Query Frame = 0


Query: 1   MESLLVLNSTPCSGDSSPLKLNK--FAAVPLRSRPSSSLRIDPLMGFGACKVSFVANTSQ 60
           MESLLVLNSTPC   +SPLK +K   AAVPLRSRPSSSLR+DPLMGFG CKVSFVANT Q
Sbjct: 1   MESLLVLNSTPC---ASPLKPHKPIAAAVPLRSRPSSSLRLDPLMGFGGCKVSFVANTFQ 60

Query: 61  RNGFLSPYRKCSLGRRVLAFAASHEESHSETQGEKDGKDFNFDVEKAEELWKNALDSFKE 120
           RNGFLS YRKC+L RRVLAFAASHEESHSETQG+K+GKD +FD EKA+ELWKNALDSFKE
Sbjct: 61  RNGFLSSYRKCTLSRRVLAFAASHEESHSETQGKKEGKDLDFDAEKAQELWKNALDSFKE 120

Query: 121 QALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIATV 180
           QA+KMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEE +EYI + 
Sbjct: 121 QAVKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEESKEYITSA 180

Query: 181 AENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIRF 240
            EN PEE+KEIVETFTSPTDDL+DIS+VHEFYYGIPYGLVLS GGF+SFMLTGSLAAIRF
Sbjct: 181 TENYPEEIKEIVETFTSPTDDLSDISKVHEFYYGIPYGLVLSVGGFVSFMLTGSLAAIRF 240

Query: 241 GVILGGALLALSILSLRAYKRGQSLPLALKGQSGQ 274
           GVILGGALL LSILSL++YKRGQSLPLALKGQ+ Q
Sbjct: 241 GVILGGALLVLSILSLQSYKRGQSLPLALKGQAVQ 272

[Close]

BLAST of Lsi01G016180.1 vs. TAIR 10
Match: AT2G38550.1 (Transmembrane proteins 14C )

HSP 1 Score: 191.8 bits (486), Expect = 7.3e-49
Identity = 111/219 (50.68%), Postives = 155/219 (70.78%), Query Frame = 0


Query: 59  RNGFLSPYRKCSLGRRVLAFAASHEES-HSETQGEKDGKDFNFDVEKAEELWKNALDSFK 118
           R GF SP  +  L R V+AFAASHE+S  S  +  K+  D + + + ++E WK  L+SFK
Sbjct: 57  RFGFTSP--EVLLNRSVVAFAASHEDSGESGVEVGKEKSDIDVEDDTSKEAWKQTLESFK 116

Query: 119 EQALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEYIAT 178
           EQ  KM+++S +AY   S+KA+  L ETS+ L+IQA+KAK++L    +  SEEG+EYI  
Sbjct: 117 EQVSKMQSVSSEAYSVNSQKAMTVLKETSEQLRIQAEKAKEELGTKAKVVSEEGREYILK 176

Query: 179 VAENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLAAIR 238
            AE  P +VKEIVE F S T+DL ++SR ++F+ GIPYGL+L  GGF++FM++GS+ AIR
Sbjct: 177 AAEESPSDVKEIVEAFAS-TEDLKNVSRANDFHVGIPYGLLLLVGGFINFMVSGSIPAIR 236

Query: 239 FGVILGGALLALSILSLRAYKRGQSLPLALKGQSGQSAI 277
           FGVILGGAL ALS+ SL+++++G+S    LKGQ    AI
Sbjct: 237 FGVILGGALFALSLASLKSHRKGESSTKFLKGQMAIVAI 272

[Close]

BLAST of Lsi01G016180.1 vs. TAIR 10
Match: AT3G57280.1 (Transmembrane proteins 14C )

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-07
Identity = 46/162 (28.40%), Postives = 82/162 (50.62%), Query Frame = 0


Query: 115 SFKEQALKMKAISKDAYDEYSEKALVALNETSKLLKIQADKAKDDLALIVQEFSEEGQEY 174
           SF    ++ ++    + D  S +   +L+ T+++ K   +K     + +         + 
Sbjct: 24  SFPCPMIRPQSFVVKSVDGNSSETPASLSYTAEVSKPVVEKTSKPYSTV---------DE 83

Query: 175 IATVAENCPEEVKEIVETFTSPTDDLTDISRVHEFYYGIPYGLVLSFGGFLSFMLTGSLA 234
            AT  E+  E V+E V T           +++H+F +GIPYG ++  GG L F  + +L 
Sbjct: 84  TATNKESITEPVEEDVAT------QPIRAAKIHDFCFGIPYGGLVVSGGLLGFAFSRNLT 143

Query: 235 AIRFGVILGGALLALSILSLRAYKRGQSLPLALKGQSGQSAI 277
           ++  GV+ GG LLALS LSL+ ++ G+S    + GQ+  SA+
Sbjct: 144 SLSTGVLYGGGLLALSTLSLKIWREGKSSFPYILGQAVLSAV 170

The following BLAST results are available for this feature:

BLAST of Lsi01G016180.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZVH7	1.0e-47	50.68	Protein FATTY ACID EXPORT 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FA...	[more]
Q93V66	2.6e-06	28.40	Protein FATTY ACID EXPORT 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FA...	[more]

BLAST of Lsi01G016180.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2W3	3.2e-124	87.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G334130 PE=3 SV=1	[more]
A0A5D3CEP9	5.6e-121	86.18	Protein FATTY ACID EXPORT 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3CIV0	9.5e-121	86.45	protein FATTY ACID EXPORT 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1035014...	[more]
A0A5A7SGY8	1.6e-120	85.82	Protein FATTY ACID EXPORT 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A6J1HP56	5.6e-113	80.81	protein FATTY ACID EXPORT 3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of Lsi01G016180.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883685.1	4.7e-130	92.25	protein FATTY ACID EXPORT 3, chloroplastic [Benincasa hispida]	[more]
XP_004142682.1	6.5e-124	87.18	protein FATTY ACID EXPORT 3, chloroplastic [Cucumis sativus] >KGN54451.1 hypothe...	[more]
TYK09762.1	1.2e-120	86.18	protein FATTY ACID EXPORT 3 [Cucumis melo var. makuwa]	[more]
XP_008463262.1	2.0e-120	86.45	PREDICTED: protein FATTY ACID EXPORT 3, chloroplastic [Cucumis melo]	[more]
KAA0025410.1	3.3e-120	85.82	protein FATTY ACID EXPORT 3 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi01G016180.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38550.1	7.3e-49	50.68	Transmembrane proteins 14C	[more]
AT3G57280.1	1.8e-07	28.40	Transmembrane proteins 14C	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044890	TMEM14 superfamily	GENE3D	1.10.10.1740		coord: 197..278 e-value: 6.4E-6 score: 27.8
IPR005349	TMEM14 family	PFAM	PF03647	Tmemb_14	coord: 211..253 e-value: 1.3E-7 score: 32.2
IPR005349	TMEM14 family	PANTHER	PTHR12668	TRANSMEMBRANE PROTEIN 14, 15	coord: 24..276
None	No IPR available	PANTHER	PTHR12668:SF43	TRANSMEMBRANE PROTEIN 14 HOMOLOG	coord: 24..276

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi01G016180	Lsi01G016180	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G016180.1.exon.1	Lsi01G016180.1.exon.1	exon
Lsi01G016180.1.exon.2	Lsi01G016180.1.exon.2	exon
Lsi01G016180.1.exon.3	Lsi01G016180.1.exon.3	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G016180.1.five_prime_UTR.1	Lsi01G016180.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G016180.1.CDS.1	Lsi01G016180.1.CDS.1	CDS
Lsi01G016180.1.CDS.2	Lsi01G016180.1.CDS.2	CDS
Lsi01G016180.1.CDS.3	Lsi01G016180.1.CDS.3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi01G016180.1	Lsi01G016180.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0015908	fatty acid transport
biological_process	GO:1905885	positive regulation of triglyceride transport
biological_process	GO:0019217	regulation of fatty acid metabolic process
cellular_component	GO:0016020	membrane
cellular_component	GO:0009706	chloroplast inner membrane
cellular_component	GO:0016021	integral component of membrane