Lsi01G011650 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G011650
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cysteine proteinase inhibitor
Location	chr01: 9802787 .. 9804337 (-)
RNA-Seq Expression	Lsi01G011650
Synteny	Lsi01G011650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAACTAATTGTTAATTAAGTTTAAAGAAGGAATGGAATAAATTGGAGTTGTATAAATTTGAAAGGAAGCACACAAAGCAAAAGCATTCCATAAGTAATAACTTTGTGGGAAAAAGTTTGTGTATTGAAAGTAAGTTAAGAAAATGTGTTCTCAGTTGCTCGGCAGTTATAGTCCATGTGAAGATCCCGAAGGCCCGCACGTAAAAGAAGTAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTCATGTGTTAAGTTGTGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTAGTTAAGGATGAAAAAAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGAAGCTCATTTACTTTAAACCTTACAAGGTATGCTTTTTCTTATAGCACTTACATGCTTTCTTTTGCAACCCTTTTCAAATTTGTTATTTGTATCTATATTTGGTTACTTAATGAAGTGTTCTCTAACTGATGAAACAAAATAAAAATCATAAATTGAGTTTGGTAAAGTTGAGTCTATTTTATTCAAATTAAAAATAGGATGATATATATTAGTTTGTACTCGGATAGTAGAAGCATGCATGACATATATAGTTGAATTTTGGGATTCAAAATTTCTTTTGTCTTAATAAAGCTATTTTTCAGTCTTAATGATCATTTAAGAAATTAGTACGCAAAAAGTGGAATAAAAATTGTAGTAGTACAAATTTGAAAATCATTCCATATTTGGTTTAACTTTTCAAATGTTTACTTTTGAAATAAGTTATTTTAAGAATAATTCAAATATTTGGTAACGGCTTAAAATAGCTTTTGAAATACTCTCAAACTATGTTTCAAACATTTTTTTTTAATCAAAAAAGAGTTTAAATAATAATGATTTTTTTTAAAAAAAATATTTTTTCTAGACAATCCAAATGTCTAAGAGGCTGTTTGGGGGACAGTAATTGGATGGGTTTAGGTTGTAACGTAATTTAAAAATCTACGTTTGAATTAAACATTTTAGGCTCGATTTGTAATACTAAACTCATTCCGTTCTCACAGTTCTCAACCTAACTCTCTTTCCCACCGTTTTCATGCTTCACGATCTCATTTTCGTTATGTCATAAATTCCTCACACATTCCAATACCCCTCAGATTTCTAATAATTTGATAACATTTCAGCCCCAAACAATCCCTAATTGTGTATATTGAAAGTAAGGAAAATGCGTTCTCAGGTAGTTGGCGGTTATGGTCCATGTAAGGATCCTGAAGGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGCTAAGGATGATAAAAATCATGAGGGAAAATATGAGGCTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

mRNA sequence

AAAAAACTAATTGTTAATTAAGTTTAAAGAAGGAATGGAATAAATTGGAGTTGTATAAATTTGAAAGGAAGCACACAAAGCAAAAGCATTCCATAAGTAATAACTTTGTGGGAAAAAGTTTGTGTATTGAAAGTAAGTTAAGAAAATGTGTTCTCAGTTGCTCGGCAGTTATAGTCCATGTGAAGATCCCGAAGGCCCGCACGTAAAAGAAGTAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTCATGTGTTAAGTTGTGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTAGTTAAGGATGAAAAAAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGAAGCTCATTTACTTTAAACCTTACAAGGATCCTGAAGGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGCTAAGGATGATAAAAATCATGAGGGAAAATATGAGGCTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

Coding sequence (CDS)

ATGTGTTCTCAGTTGCTCGGCAGTTATAGTCCATGTGAAGATCCCGAAGGCCCGCACGTAAAAGAAGTAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTCATGTGTTAAGTTGTGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTAGTTAAGGATGAAAAAAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGAAGCTCATTTACTTTAAACCTTACAAGGATCCTGAAGGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCGCAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGCTAAGGATGATAAAAATCATGAGGGAAAATATGAGGCTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

Protein sequence

MCSQLLGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHCLTLIHVLSCESQVVAGVNYRLVLLVKDEKNHEKVYETVVWEKIWENFRKLIYFKPYKDPEGPHVKEIAEWAVAEYNKGGHCLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLISFKPLLLC

Homology

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BLAST of Lsi01G011650 vs. ExPASy Swiss-Prot
Match: P37842 (Multicystatin OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 8.9e-21
Identity = 58/185 (31.35%), Postives = 95/185 (51.35%), Query Frame = 0


Query: 3   SQLLGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLL 62
           S + G      +P     +E+A +A+ +YNK  +  L  +  L+ + QVVAG+ Y + L 
Sbjct: 95  STMPGGIVNVPNPNNTKFQELARFAIQDYNKKQNAHLEFVENLNVKEQVVAGIMYYITLA 154

Query: 63  VKDEKNHEKVYETVVWEKIWENFRKLIYFKPYKD-------------PEGPHVKEIAEWA 122
             D+   +K+Y+  +W K WE+F+K++ FK   D             P  P  +++A +A
Sbjct: 155 ATDDAGKKKIYKAKIWVKEWEDFKKVVEFKLVGDDIAKLGGITDVPFPNNPEFQDLARFA 214

Query: 123 VAEYNKGGHC-LTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRK 173
           +  YNK  +  L  +  L  + QVVAG+ Y  + LA  D   +  YE  +W K WE F+K
Sbjct: 215 IQVYNKKENVHLEFVENLNVKQQVVAGMMY-YITLAAIDAGKKKIYETKIWVKEWEDFKK 274

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BLAST of Lsi01G011650 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 4.4e-12
Identity = 39/84 (46.43%), Postives = 57/84 (67.86%), Query Frame = 0


Query: 90  FKPYKDPEGPHVKEIAEWAVAEYNKGGHC-LTLISVLKCESQVVAGVNYRLVLLAKDDKN 149
           ++P ++     V+++A++AV+E+NK  +  L   SV++  +QVVAG NYRLV+ AKD   
Sbjct: 32  WRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAKDGA- 91

Query: 150 HEGKYEAVVWEKIWEHFRKLISFK 173
             G YEAVVW+K W HFR L SF+
Sbjct: 92  VVGNYEAVVWDKPWMHFRNLTSFR 114

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BLAST of Lsi01G011650 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.4e-12
Identity = 42/92 (45.65%), Postives = 60/92 (65.22%), Query Frame = 0


Query: 85  RKLIY---FKPYKDPEGPHVKEIAEWAVAEYNKGGHC-LTLISVLKCESQVVAGVNYRLV 144
           RKL+    ++P +      V+++A++AV+E+NK  +  L   SV++  +QVVAG NYRLV
Sbjct: 24  RKLVAAGGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLV 83

Query: 145 LLAKDDKNHEGKYEAVVWEKIWEHFRKLISFK 173
           + AKD     G YEAVVW+K W HFR L SF+
Sbjct: 84  IAAKDGA-VVGNYEAVVWDKPWMHFRNLTSFR 114

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BLAST of Lsi01G011650 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 3.7e-11
Identity = 37/88 (42.05%), Postives = 50/88 (56.82%), Query Frame = 0


Query: 6   LGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKD 65
           +G +SP  +   P V E+ E+AV+EYNK     L    V+S E+QVV+G NYRL +   D
Sbjct: 28  VGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAAND 87

Query: 66  EKNHEKVYETVVWEKIWENFRKLIYFKP 93
                K Y  +VW+K W  FR L  F+P
Sbjct: 88  GDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

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BLAST of Lsi01G011650 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-11
Identity = 40/83 (48.19%), Postives = 52/83 (62.65%), Query Frame = 0


Query: 95  DPEGPHVKEIAEWAVAEYNKG--GHCLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGK 154
           D   PHV+E+  WAVAE ++G     LT   V   E QVVAGVNYRL L A      +G+
Sbjct: 34  DINDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGR 93

Query: 155 YEAVVWEK-IWEHFRKLISFKPL 175
           YEAVV+E+    + RKL+SF+P+
Sbjct: 94  YEAVVYEQDPRSNARKLVSFEPI 116

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BLAST of Lsi01G011650 vs. ExPASy TrEMBL
Match: A0A4S8KBT4 (Uncharacterized protein OS=Musa balbisiana OX=52838 GN=C4D60_Mb04t13670 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.6e-36
Identity = 95/180 (52.78%), Postives = 118/180 (65.56%), Query Frame = 0


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E+NK     LTL  V   E+QVVAG NYRLVL V+D 
Sbjct: 33  GGWTAIEDLKDPHVREIAEFAVSEHNKLEKTNLTLRKVEGGETQVVAGTNYRLVLEVRDG 92

Query: 67  KNHEKVYETVVWEKIWENFRKLIYF---------KPYKDPEGPHVKEIAEWAVAEYN-KG 126
                 YE VVWEK WENF++L  F          P K+   P V EIAE+A+AE+N + 
Sbjct: 93  SGASARYEAVVWEKPWENFKQLASFHKLVIVGGWTPIKNISDPDVGEIAEFAIAEHNHEA 152

Query: 127 GHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISFKPL 175
           G  LTL  V+K E+QVVAG+NY+LVL AKD       +YEAVVWEK WEHFR+L SFK L
Sbjct: 153 GSNLTLCKVVKGETQVVAGINYKLVLEAKDGGVGVMSEYEAVVWEKTWEHFRRLTSFKLL 212

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BLAST of Lsi01G011650 vs. ExPASy TrEMBL
Match: A0A426WY30 (Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00051059 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 6.6e-35
Identity = 94/177 (53.11%), Postives = 116/177 (65.54%), Query Frame = 0


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E+NK     LTL  V   ESQVVAG+NYRLVL V+D 
Sbjct: 54  GGWTAIEDLKDPHVREIAEFAVSEHNKLEKTNLTLRKVEGGESQVVAGMNYRLVLEVRDG 113

Query: 67  KNHEKVYETVVWEKIWENFRKLIYFK---------PYKDPEGPHVKEIAEWAVAEYN-KG 126
                 YE VVWEK WE+FR+L  F+         P ++   P V EIAE+AVAE+N + 
Sbjct: 114 SGASVRYEAVVWEKTWEHFRELTSFRKLVIVGGWTPIENIGDPDVAEIAEFAVAEHNHEA 173

Query: 127 GHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISF 172
           G  L L  V+K E+QVVAG NY+LVL AKD       +YEAVVWEK WEHFRKL SF
Sbjct: 174 GSNLKLCKVVKGETQVVAGTNYKLVLGAKDGGAGVVSEYEAVVWEKTWEHFRKLTSF 230

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BLAST of Lsi01G011650 vs. ExPASy TrEMBL
Match: A0A444FRQ3 (Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=GW17_00010346 PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 8.6e-35
Identity = 94/177 (53.11%), Postives = 116/177 (65.54%), Query Frame = 0


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E+NK     LTL  V   ESQVVAG+NYRLVL V+D 
Sbjct: 54  GGWTAIEDLKDPHVREIAEFAVSEHNKLEKTNLTLRKVEGGESQVVAGMNYRLVLEVRDG 113

Query: 67  KNHEKVYETVVWEKIWENFRKLIYFK---------PYKDPEGPHVKEIAEWAVAEYN-KG 126
                 YE VVWEK WE+FR+L  F+         P ++   P V EIAE+AVAE+N + 
Sbjct: 114 SGASVRYEAVVWEKTWEHFRELTSFRKLVIVGGWTPIENIGDPDVAEIAEFAVAEHNHEA 173

Query: 127 GHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISF 172
           G  L L  V+K E+QVVAG NY+LVL AKD       +YEAVVWEK WEHFRKL SF
Sbjct: 174 GTNLKLCKVVKGETQVVAGTNYKLVLGAKDGGAGVVSEYEAVVWEKTWEHFRKLTSF 230

[Close]

BLAST of Lsi01G011650 vs. ExPASy TrEMBL
Match: A0A426Z5E1 (Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00014443 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 1.1e-34
Identity = 94/181 (51.93%), Postives = 117/181 (64.64%), Query Frame = 0


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E+N      LTL  V   ESQVVAG+NYRLVL V+D 
Sbjct: 70  GGWTAIEDLKDPHVREIAEFAVSEHNMLEKTNLTLRKVEGGESQVVAGMNYRLVLEVRDG 129

Query: 67  KNHEKVYETVVWEKIWENFRKLIYFK----------PYKDPEGPHVKEIAEWAVAEYN-K 126
                 YE VVWEK WE+FRKL  ++          P K+   P V EIAE+AVAE+N +
Sbjct: 130 SGASARYEAVVWEKTWEHFRKLTSYRKLVLAVGGWTPIKNISDPDVGEIAEFAVAEHNHE 189

Query: 127 GGHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISFKP 175
               LTL  V+K E+QVVAG+NY+LVL AKD       +YEAVVWEK WEH RKL SF+ 
Sbjct: 190 AATNLTLCKVVKGETQVVAGINYKLVLEAKDGGVGVVSEYEAVVWEKAWEHLRKLTSFEL 249

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BLAST of Lsi01G011650 vs. ExPASy TrEMBL
Match: V4T622 (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10002632mg PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.5e-34
Identity = 88/182 (48.35%), Postives = 117/182 (64.29%), Query Frame = 0


Query: 5   LLGSYSPCEDPEGPHVKEVAEWAVAEYNK-GGHCLTLIHVLSCESQVVAGVNYRLVLLVK 64
           +LG + P EDP+  HV E+ ++AV EYNK     L    V   E+QVV+G NYRL+L+VK
Sbjct: 1   MLGGWKPIEDPKEKHVMEIGQFAVTEYNKRSKSALKFESVEKGETQVVSGTNYRLILVVK 60

Query: 65  DEKNHEKVYETVVWEKIWENFRKLIYF---------KPYKDPEGPHVKEIAEWAVAEYNK 124
           D  + +K +E VV EK WE+F+ L  F         KP +DP+  HV EI ++AV EYNK
Sbjct: 61  DGPSTKK-FEAVVLEKPWEHFKSLTSFKPMPMLGGWKPIEDPKEKHVMEIGQFAVTEYNK 120

Query: 125 GGH-CLTLISVLKCESQVVAGVNYRLVLLAKDDKNHEGKYEAVVWEKIWEHFRKLISFKP 176
                L   SV K E+QVV+G NYRL+L+ KD  + + K+EAVVWEK WEHF+ L SFKP
Sbjct: 121 RSKGALKFESVEKGETQVVSGTNYRLILVVKDGPSTK-KFEAVVWEKPWEHFKSLTSFKP 180

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BLAST of Lsi01G011650 vs. NCBI nr
Match: KAE8647458.1 (hypothetical protein Csa_004041 [Cucumis sativus])

HSP 1 Score: 167.2 bits (422), Expect = 1.3e-37
Identity = 87/178 (48.88%), Postives = 119/178 (66.85%), Query Frame = 0


Query: 9   YSPCEDPEGPHVKEVAEWAVAEYNKG-GHCLTLIHVLSCESQVVAGVNYRLVLLVKDEKN 68
           YSP EDP+   +KE+AEW VAE+NK  G  L  I +  CE Q+V GVN+R  L  KDE +
Sbjct: 10  YSPVEDPQSQRMKELAEWIVAEHNKNEGTHLKFIRIWKCEVQIVNGVNHRFTLTAKDEND 69

Query: 69  HEKVYETVVWEKIWENFRKLIY------FKPYKDPEGPHVKEIAEWAVAEYN-KGGHCLT 128
           +E  Y  VV E+ W++ ++L+       + P KDP+ P +KE+AEWAVAE+N K G  L 
Sbjct: 70  YEAAYMAVVLEQQWKHLKELMASSEPGGYTPVKDPQSPRMKELAEWAVAEHNKKEGTHLR 129

Query: 129 LISVLKCESQVVAGVNYRLVLLAKDDKNH--EGKYEAVVWEKIWEHFRKLISFKPLLL 177
            IS+LKCESQ+V GVNYR  L AKD+ ++   G Y A+V+E++ EH ++L+ FK LLL
Sbjct: 130 FISILKCESQIVNGVNYRFTLTAKDESDNCLPGNYMAIVYEQLSEHLKELVFFKQLLL 187

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: THU72580.1 (hypothetical protein C4D60_Mb04t13670 [Musa balbisiana])

HSP 1 Score: 161.0 bits (406), Expect = 9.4e-36
Identity = 95/180 (52.78%), Postives = 118/180 (65.56%), Query Frame = 0


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E+NK     LTL  V   E+QVVAG NYRLVL V+D 
Sbjct: 33  GGWTAIEDLKDPHVREIAEFAVSEHNKLEKTNLTLRKVEGGETQVVAGTNYRLVLEVRDG 92

Query: 67  KNHEKVYETVVWEKIWENFRKLIYF---------KPYKDPEGPHVKEIAEWAVAEYN-KG 126
                 YE VVWEK WENF++L  F          P K+   P V EIAE+A+AE+N + 
Sbjct: 93  SGASARYEAVVWEKPWENFKQLASFHKLVIVGGWTPIKNISDPDVGEIAEFAIAEHNHEA 152

Query: 127 GHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISFKPL 175
           G  LTL  V+K E+QVVAG+NY+LVL AKD       +YEAVVWEK WEHFR+L SFK L
Sbjct: 153 GSNLTLCKVVKGETQVVAGINYKLVLEAKDGGVGVMSEYEAVVWEKTWEHFRRLTSFKLL 212

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: RRT32118.1 (hypothetical protein B296_00051059 [Ensete ventricosum])

HSP 1 Score: 157.1 bits (396), Expect = 1.4e-34
Identity = 94/177 (53.11%), Postives = 116/177 (65.54%), Query Frame = 0


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E+NK     LTL  V   ESQVVAG+NYRLVL V+D 
Sbjct: 54  GGWTAIEDLKDPHVREIAEFAVSEHNKLEKTNLTLRKVEGGESQVVAGMNYRLVLEVRDG 113

Query: 67  KNHEKVYETVVWEKIWENFRKLIYFK---------PYKDPEGPHVKEIAEWAVAEYN-KG 126
                 YE VVWEK WE+FR+L  F+         P ++   P V EIAE+AVAE+N + 
Sbjct: 114 SGASVRYEAVVWEKTWEHFRELTSFRKLVIVGGWTPIENIGDPDVAEIAEFAVAEHNHEA 173

Query: 127 GHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISF 172
           G  L L  V+K E+QVVAG NY+LVL AKD       +YEAVVWEK WEHFRKL SF
Sbjct: 174 GSNLKLCKVVKGETQVVAGTNYKLVLGAKDGGAGVVSEYEAVVWEKTWEHFRKLTSF 230

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: RWW25321.1 (hypothetical protein GW17_00010346 [Ensete ventricosum] >RWW36179.1 hypothetical protein BHE74_00058818 [Ensete ventricosum] >RZS28419.1 hypothetical protein BHM03_00062003 [Ensete ventricosum])

HSP 1 Score: 156.8 bits (395), Expect = 1.8e-34
Identity = 94/177 (53.11%), Postives = 116/177 (65.54%), Query Frame = 0


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E+NK     LTL  V   ESQVVAG+NYRLVL V+D 
Sbjct: 54  GGWTAIEDLKDPHVREIAEFAVSEHNKLEKTNLTLRKVEGGESQVVAGMNYRLVLEVRDG 113

Query: 67  KNHEKVYETVVWEKIWENFRKLIYFK---------PYKDPEGPHVKEIAEWAVAEYN-KG 126
                 YE VVWEK WE+FR+L  F+         P ++   P V EIAE+AVAE+N + 
Sbjct: 114 SGASVRYEAVVWEKTWEHFRELTSFRKLVIVGGWTPIENIGDPDVAEIAEFAVAEHNHEA 173

Query: 127 GHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISF 172
           G  L L  V+K E+QVVAG NY+LVL AKD       +YEAVVWEK WEHFRKL SF
Sbjct: 174 GTNLKLCKVVKGETQVVAGTNYKLVLGAKDGGAGVVSEYEAVVWEKTWEHFRKLTSF 230

[Close]

BLAST of Lsi01G011650 vs. NCBI nr
Match: XP_009396392.1 (PREDICTED: cysteine proteinase inhibitor 1-like [Musa acuminata subsp. malaccensis] >CAG1842117.1 unnamed protein product [Musa acuminata subsp. malaccensis])

HSP 1 Score: 156.8 bits (395), Expect = 1.8e-34
Identity = 94/180 (52.22%), Postives = 117/180 (65.00%), Query Frame = 0


Query: 7   GSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDE 66
           G ++  ED + PHV+E+AE+AV+E NK     LTL  V   E+QVVAG NYRLVL V+D 
Sbjct: 33  GGWTAIEDLKDPHVREIAEFAVSENNKLEKTNLTLRKVEGGETQVVAGTNYRLVLEVRDG 92

Query: 67  KNHEKVYETVVWEKIWENFRKLIYF---------KPYKDPEGPHVKEIAEWAVAEYN-KG 126
                 YE VVWEK WE+F++L  F          P K+   P V EIAE+AVAE+N + 
Sbjct: 93  SGASARYEAVVWEKPWESFKQLASFHKLVIVGGWTPIKNISDPDVGEIAEFAVAEHNHEA 152

Query: 127 GHCLTLISVLKCESQVVAGVNYRLVLLAKD-DKNHEGKYEAVVWEKIWEHFRKLISFKPL 175
           G  LTL  V+K E+QVVAG+NY+LV+ AKD       +YEAVVWEK WEHFR+L SFK L
Sbjct: 153 GTNLTLCKVVKGETQVVAGINYKLVIEAKDGGVGVVSEYEAVVWEKTWEHFRRLTSFKLL 212

[Close]

BLAST of Lsi01G011650 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 2.7e-12
Identity = 37/88 (42.05%), Postives = 50/88 (56.82%), Query Frame = 0


Query: 6   LGSYSPCEDPEGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKD 65
           +G +SP  +   P V E+ E+AV+EYNK     L    V+S E+QVV+G NYRL +   D
Sbjct: 28  VGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAAND 87

Query: 66  EKNHEKVYETVVWEKIWENFRKLIYFKP 93
                K Y  +VW+K W  FR L  F+P
Sbjct: 88  GDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Lsi01G011650 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-10
Identity = 35/85 (41.18%), Postives = 49/85 (57.65%), Query Frame = 0


Query: 91  KPYKDPEGPHVKEIAEWAVAEYNK-GGHCLTLISVLKCESQVVAGVNYRLVLLAKDDKNH 150
           KP K+   P V  +A++A+ E+NK     L  + V++  +QVV+G  Y L + AKD    
Sbjct: 33  KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGK 92

Query: 151 EGKYEAVVWEKIWEHFRKLISFKPL 175
              YEAVV EK+W H + L SFK L
Sbjct: 93  IKNYEAVVVEKLWLHSKSLESFKAL 117

[Close]

BLAST of Lsi01G011650 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 55.1 bits (131), Expect = 6.8e-08
Identity = 36/89 (40.45%), Postives = 50/89 (56.18%), Query Frame = 0


Query: 6   LGSYSPCEDP--EGPHVKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLV 65
           LG +S  ++    G  + ++A +AV E+N+  +  L L  VL    QVVAG  YRL L V
Sbjct: 45  LGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEV 104

Query: 66  KDEKNHEKVYETVVWEKIWENFRKLIYFK 92
             E   +K+YE  VW K W NF++L  FK
Sbjct: 105 -IEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]

BLAST of Lsi01G011650 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 52.0 bits (123), Expect = 5.7e-07
Identity = 31/77 (40.26%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 20  VKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDEKNHEKVYETVVWE 79
           V+ +A +AV E+NK  +  L    V+  + QVVAG  + L L +  E   +K+YE  VW 
Sbjct: 52  VESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL-EAGQKKLYEAKVWV 111

Query: 80  KIWENFRKLIYFKPYKD 96
           K W NF++L  FKP  D
Sbjct: 112 KPWLNFKELQEFKPASD 127

[Close]

BLAST of Lsi01G011650 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 52.0 bits (123), Expect = 5.7e-07
Identity = 31/77 (40.26%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 20 VKEVAEWAVAEYNKGGHC-LTLIHVLSCESQVVAGVNYRLVLLVKDEKNHEKVYETVVWE 79
          V+ +A +AV E+NK  +  L    V+  + QVVAG  + L L +  E   +K+YE  VW 
Sbjct: 19 VESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL-EAGQKKLYEAKVWV 78

Query: 80 KIWENFRKLIYFKPYKD 96
          K W NF++L  FKP  D
Sbjct: 79 KPWLNFKELQEFKPASD 94

The following BLAST results are available for this feature:

BLAST of Lsi01G011650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P37842	8.9e-21	31.35	Multicystatin OS=Solanum tuberosum OX=4113 PE=1 SV=1	[more]
P86472	4.4e-12	46.43	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q6TPK4	4.4e-12	45.65	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
Q41916	3.7e-11	42.05	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
Q10Q47	4.9e-11	48.19	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]

BLAST of Lsi01G011650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A4S8KBT4	4.6e-36	52.78	Uncharacterized protein OS=Musa balbisiana OX=52838 GN=C4D60_Mb04t13670 PE=3 SV=...	[more]
A0A426WY30	6.6e-35	53.11	Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00051059 PE=3 SV=1	[more]
A0A444FRQ3	8.6e-35	53.11	Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=GW17_00010346 PE=3 SV=1	[more]
A0A426Z5E1	1.1e-34	51.93	Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00014443 PE=3 SV=1	[more]
V4T622	1.5e-34	48.35	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10002632mg PE=4 ...	[more]

BLAST of Lsi01G011650 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647458.1	1.3e-37	48.88	hypothetical protein Csa_004041 [Cucumis sativus]	[more]
THU72580.1	9.4e-36	52.78	hypothetical protein C4D60_Mb04t13670 [Musa balbisiana]	[more]
RRT32118.1	1.4e-34	53.11	hypothetical protein B296_00051059 [Ensete ventricosum]	[more]
RWW25321.1	1.8e-34	53.11	hypothetical protein GW17_00010346 [Ensete ventricosum] >RWW36179.1 hypothetical...	[more]
XP_009396392.1	1.8e-34	52.22	PREDICTED: cysteine proteinase inhibitor 1-like [Musa acuminata subsp. malaccens...	[more]

BLAST of Lsi01G011650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	2.7e-12	42.05	Cystatin/monellin superfamily protein	[more]
AT4G16500.1	1.9e-10	41.18	Cystatin/monellin superfamily protein	[more]
AT5G05110.1	6.8e-08	40.45	Cystatin/monellin family protein	[more]
AT3G12490.2	5.7e-07	40.26	cystatin B	[more]
AT3G12490.1	5.7e-07	40.26	cystatin B	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 4..93 e-value: 5.3E-9 score: 45.9 coord: 96..174 e-value: 1.4E-5 score: 34.5
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 94..173 e-value: 1.8E-27 score: 95.5 coord: 16..92 e-value: 2.9E-24 score: 85.2
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 94..173 e-value: 1.26928E-13 score: 61.7212
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 11..92 e-value: 1.45637E-13 score: 61.7212
None	No IPR available	GENE3D	3.10.450.10		coord: 95..175 e-value: 1.9E-25 score: 90.9
None	No IPR available	GENE3D	3.10.450.10		coord: 2..94 e-value: 2.8E-26 score: 93.6
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 90..174 coord: 4..92
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 4..92
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 90..174
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 4..95
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 97..174

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G011650.1	Lsi01G011650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity