Tan0017080 (gene) Snake gourd v1

Overview
NameTan0017080
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionthermospermine synthase ACAULIS5-like
LocationLG10: 7115063 .. 7118573 (+)
RNA-Seq ExpressionTan0017080
SyntenyTan0017080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTTCCCATTGCACGCCCTTCTCTCTCTACACTTTCACTCTTTCTCTCTCTCTAACCAAAATTTTGAAACACATTCTTGAAGAAATGAATAACCTGGATTTTCGGTAATTGTCACTCTTTCAATCATCATCTTTTATCCTTTTCTGTTGAACAAAAAAAAGAAAAAGAAAAAAAAAAAAGAAAATTTGAATATGATATGCATTTGGACAAAGCAATCTTCTTCCCTTTTTTTTTTCACCAATTTTCCATTTATTTTCTTCATATCCCAATTACCCACTGGTTTATTTGAGAATTTCAACAACTGGGCTGTTTGATTCTTAAGAAAAGTTTGAATCTTTACCAAATTTATGAAGTTTTTAAGTGAATTTGATTCTTCTTTGTCCATAAATTCTTTAATATGCATAAGAAACGTGTAATGATTGTCATTTATTAGATTGGATACAAGGAAGAAAAGAAGAAGAAGAAGACAGGAAATGAAACCACGCGACCAAATCATCCTTAGAACATCACAAACATTGGTCTCTGAAACTTTACGAAAATCCAAAAGGATTTTTTTTTTCTCTTTTCTTTTGGAGATTACTTTTCAACTCTCTCTCTCTCTCCTTTTCCTTTCCCCCTTCTTCCTTAAATACCCCCTTCTTCCTTCCTTTCTTCTCCATCAAGATTTCTAACCCTTCTCTGTTCTTCTCTCCTCTGAGTTTCTTCTCTCTGCAACAACAATGGCTGATGTTTCCTCTGCCAATTTGGTTAACAATGGCAACGAACTCATTGAGAAGGGTCATTCCCTAAATGGGTATCGAAAGAGCTGTTGGTACGAGGAGGAGATTGAAGAGAATTTAAGATGGTCGTTTGCTCTTAATAGGTACTTGTTTTTGTTACATTTTAAATCAACTTTGTCCCAAAAAAAAAAAAAACTGTCCTGTTTTCTGAATCCTCTGTTCATTTTCATCAATGTTTTTCAAGTTCATTCAGCTTTGTTTTCTAAAATTTTTGCCTTTTGGTTTGTACAGTATTCATTAAAAGTGTCTTAAGGTAAAAGTGTCATTGTTTTTTTGATTATTTTAAGTGGGGTTCTTACATTTGGCTGTTTTGCTAGTTGAAATGGGGTTGAAATCTTTAAAAAGTAAAAAGCTTTTTTCCCTTCCCACTTCAAATCAAGCTCAATAAAATAATCCATTGGTCTTGGAAAAGATAGACAAGAAACCTTAAAACTAAAAAAAGGAGTTTAGTTTGGGGATTGTTCTTCACTTCTTGTCTGCCAAAATCTACATACTTCGTGATGAACTTTTTCTTCTTTTGTTATAATCTTGTAGCATTCTGCATACTGGAGCTTCTCAGTATCAAGACATCGCGCTACTCGACACGAAGCCATTCGGGAAGGCGTTGGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAAGTACTTAGAAAGCCTAATGACTAATTTTCTTTGAACAATTTAAGTATACAAATGTTAATAACTTCCTTTTTGTAATGTTTTAAACTCAGTCCAAAGACAATCTTTATCATGGGAGGAGGAGAAGGCTCCACTGCAAGAGAGCTACTTAGACACAAGACTGTGGAGAAAGTTGTAATGTGCGACATCGATGAGGTTCGAATTACTTTCGTTTTTCTTCTTATATACGATACGAAAAAGCTCACTAAAACCATGATGGGTAACCAATGAGCAGGAAGTTGTTGATTTCTGCAAGTCATACCTGATAGTAAACAAAGAAGCTTTCTGTGATCCAAGACTCGAACTTATCATTAACGATGCCAGGTTCGATTCCGATTAGCGCATAAACAATTCGTCCTACGTTATGATTGCTATTTTGGTTGCTATAAGTTCTGGTGGGCTTGTTGCAGAGCGGAGCTTGAGAACCGAATGGAACAATATGACGTGATCGTGGGCGATCTTGCAGACCCGATTGAGGGTGGTCCTTGTTATAAACTCTATACCAAATCCTTCTATGAGTATAGTGTAAAGCCTAGATTGAAACAAGATGGAATATTTATCACTCAGGTTTGTGTAAGAGATGAAAAATTAAAGAAGTTTGTGAATTTATATGAGTTTTGGAACTCAAGTTGTTGTTGAAATCTATGCAGGCTGGTCCAGCTGGAATTTTCAGCCACACAGAAGTGTTTTCTTGCATTTACAATACTCTAAAGCAAGTTTTTAAATGTGAGCAAAACTTGAGACTATTATTGTTAATGGTTTTAGCCTAAGTTACTTTTAAGAATTTAATGGATTGACTGTGATTGTTGTTCTGTTTTTTCTTTCTAATTTGCAGATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGTAAGAAAAATGATAACTTTTCATGTTTTTGGGCTGGAAATTATGGCAAAAAATGACAAATAGAATGAGATAAAAGATCTAAAGGTTGTTCGGGTGCGTGGATCCGTTTTAAATCGAATAGATTTTCAAGGTTGTTTTTACAGTCAGTTGTGTTTGTACGTATGCACTCGAAATCACTATTTATCAGTAAGAAACAGTTCCTCCATGTGCAGAAAAGTAGGGGAAACGATAATCCGAACATGTTTTTTTCACTAAAACGATAACGACATCGGTTTCGAGTGTTTGTATGCACTCGAAATCACAATTTATCAGTAAGAAACAGTTCCTCCATGTGAACAAAAGTAGGGGAAATGATAATCCGAACATGTTTTTTTCACTAAAACGAAAACGACATCGGTTTCGAGTGTTTGTATGCACTCGAAATCACAATTTATCAGTAAGAAACAGTTCCTCCATGTGAACAAAGTAGGGGAAACGATAATCCGAACATGTTTTTTTCACTAAAACGAAAACGACATCGGTTTCTAAACTCAACTTTCAAATGGGGCTCAAAACTTTTGGAATGTTGAATTTTAAGCAGAATTTCTTGAAACTTGCAGGCCTCAGACACTCCTTTTGTCCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAAACATTCTCATCTGCCTCGACATTAAGCAAAGCGGTTCGGAAGTCGTAAGTAGACGTTTTTTTTCCTCTCCTTGTTCTTCTGATTTATTTTCAAATTTGGGAGATGTTTTAAGATTGACAAGGATTTTCGCAGGCTCGACAACGAGACGCACGTGTACACGGAAGGAACGGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTTTGATAGTTTTTGAATTAAGAAAAAGAGAAGAAGAAAGGGCCCCTAAATAAGAGAATATAGAAATCCATCATGTCAGCCTAGAAATGAAACAAATCTCCTCTGGGCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTCTTATTGTTTGTGTAGTGACTTTAAAGTTACAAATATAATAGTAATGAAAAAAG

mRNA sequence

GGTTCCCATTGCACGCCCTTCTCTCTCTACACTTTCACTCTTTCTCTCTCTCTAACCAAAATTTTGAAACACATTCTTGAAGAAATGAATAACCTGGATTTTCGATTGGATACAAGGAAGAAAAGAAGAAGAAGAAGACAGGAAATGAAACCACGCGACCAAATCATCCTTAGAACATCACAAACATTGGTCTCTGAAACTTTACGAAAATCCAAAAGGATTTTTTTTTTCTCTTTTCTTTTGGAGATTACTTTTCAACTCTCTCTCTCTCTCCTTTTCCTTTCCCCCTTCTTCCTTAAATACCCCCTTCTTCCTTCCTTTCTTCTCCATCAAGATTTCTAACCCTTCTCTGTTCTTCTCTCCTCTGAGTTTCTTCTCTCTGCAACAACAATGGCTGATGTTTCCTCTGCCAATTTGGTTAACAATGGCAACGAACTCATTGAGAAGGGTCATTCCCTAAATGGGTATCGAAAGAGCTGTTGGTACGAGGAGGAGATTGAAGAGAATTTAAGATGGTCGTTTGCTCTTAATAGCATTCTGCATACTGGAGCTTCTCAGTATCAAGACATCGCGCTACTCGACACGAAGCCATTCGGGAAGGCGTTGGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAATCCAAAGACAATCTTTATCATGGGAGGAGGAGAAGGCTCCACTGCAAGAGAGCTACTTAGACACAAGACTGTGGAGAAAGTTGTAATGTGCGACATCGATGAGGAAGTTGTTGATTTCTGCAAGTCATACCTGATAGTAAACAAAGAAGCTTTCTGTGATCCAAGACTCGAACTTATCATTAACGATGCCAGAGCGGAGCTTGAGAACCGAATGGAACAATATGACGTGATCGTGGGCGATCTTGCAGACCCGATTGAGGGTGGTCCTTGTTATAAACTCTATACCAAATCCTTCTATGAGTATAGTGTAAAGCCTAGATTGAAACAAGATGGAATATTTATCACTCAGGCTGGTCCAGCTGGAATTTTCAGCCACACAGAAGTGTTTTCTTGCATTTACAATACTCTAAAGCAAGTTTTTAAATATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGCCTCAGACACTCCTTTTGTCCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAAACATTCTCATCTGCCTCGACATTAAGCAAAGCGGTTCGGAAGTCGCTCGACAACGAGACGCACGTGTACACGGAAGGAACGGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTTTGATAGTTTTTGAATTAAGAAAAAGAGAAGAAGAAAGGGCCCCTAAATAAGAGAATATAGAAATCCATCATGTCAGCCTAGAAATGAAACAAATCTCCTCTGGGCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTCTTATTGTTTGTGTAGTGACTTTAAAGTTACAAATATAATAGTAATGAAAAAAG

Coding sequence (CDS)

ATGGCTGATGTTTCCTCTGCCAATTTGGTTAACAATGGCAACGAACTCATTGAGAAGGGTCATTCCCTAAATGGGTATCGAAAGAGCTGTTGGTACGAGGAGGAGATTGAAGAGAATTTAAGATGGTCGTTTGCTCTTAATAGCATTCTGCATACTGGAGCTTCTCAGTATCAAGACATCGCGCTACTCGACACGAAGCCATTCGGGAAGGCGTTGGTCATCGATGGAAAGCTTCAAAGTGCAGAAACTGATGAATTCATTTACCATGAATGCCTTGTTCACCCTGCACTTCTCCATCATCCCAATCCAAAGACAATCTTTATCATGGGAGGAGGAGAAGGCTCCACTGCAAGAGAGCTACTTAGACACAAGACTGTGGAGAAAGTTGTAATGTGCGACATCGATGAGGAAGTTGTTGATTTCTGCAAGTCATACCTGATAGTAAACAAAGAAGCTTTCTGTGATCCAAGACTCGAACTTATCATTAACGATGCCAGAGCGGAGCTTGAGAACCGAATGGAACAATATGACGTGATCGTGGGCGATCTTGCAGACCCGATTGAGGGTGGTCCTTGTTATAAACTCTATACCAAATCCTTCTATGAGTATAGTGTAAAGCCTAGATTGAAACAAGATGGAATATTTATCACTCAGGCTGGTCCAGCTGGAATTTTCAGCCACACAGAAGTGTTTTCTTGCATTTACAATACTCTAAAGCAAGTTTTTAAATATGTGGTGCCTTATTCTGCCCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGCCTCAGACACTCCTTTTGTCCTGAATGCTGATGATTTGGACCAGAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAAACATTCTCATCTGCCTCGACATTAAGCAAAGCGGTTCGGAAGTCGCTCGACAACGAGACGCACGTGTACACGGAAGGAACGGCGAGGTTCATATATGGATATGGAGCTGCAGCCTGCAAGCTCAACCAAGTTTGA

Protein sequence

MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIVGDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV
Homology
BLAST of Tan0017080 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 1.7e-129
Identity = 212/328 (64.63%), Postives = 268/328 (81.71%), Query Frame = 0

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 8   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 67

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 68  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 127

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E++D+IVGDL
Sbjct: 128 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 187

Query: 189 ADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 188 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 247

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 248 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 307

Query: 309 KAVRKSLDNETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 308 KTISLALEKETEVYSEENARFIHGHGVA 335

BLAST of Tan0017080 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.6e-58
Identity = 120/277 (43.32%), Postives = 174/277 (62.82%), Query Frame = 0

Query: 45  ALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK 104
           A+  ++    + YQ   L++   FGKAL++DGK+QS  TDEFIYHE LVHP LL   NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 TIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIIN 164
            I I+GGGEG+T RE+L+HKT++ V M DID  V+DF K YL   ++ AF +P+ +L+I 
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DARAELENRMEQYDVIVGDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAG 224
           D    ++   E++D +V DL DPI+  P   LYTK FYE  VK R+ + GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYE-EVK-RISKWGIVTQATSPS- 199

Query: 225 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASD--TPFVLNADDLDQRIK 284
            FS  E FS IYNT+K VFK V     ++P+F   WG+V ASD   P   + ++++ RIK
Sbjct: 200 -FS-LETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIK 259

Query: 285 QRIKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYT 319
           +RI G  +++DG+T  +  ++ K +R+ L+ E  + T
Sbjct: 260 ERIDGSLRFYDGETHITMFSIPKHIREVLEKENKIST 291

BLAST of Tan0017080 vs. ExPASy Swiss-Prot
Match: A2BJU2 (Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5) OX=415426 GN=speE2 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 120/272 (44.12%), Postives = 170/272 (62.50%), Query Frame = 0

Query: 49  ILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFI 108
           I   GA++YQ+  + +    GKALV+DGK+QS+  DE  YHE LVHP LL H  P+ + +
Sbjct: 27  IYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQCPRKVLV 86

Query: 109 MGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIINDARA 168
           +GGGEG+T RE+LRH  VE V M DIDEE+V+  K +L   ++ AF   +LEL+I D R 
Sbjct: 87  IGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRR 146

Query: 169 ELENRMEQYDVIVGDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSH 228
            +EN   +YD I+ DL DP+EGGP  +LYT  FY  +VK  L+  G  +TQA    +   
Sbjct: 147 YVENCHRKYDAIILDLVDPMEGGPAARLYTLEFYR-AVKGCLRPGGAVVTQATSPTL--S 206

Query: 229 TEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDT--PFVLNADDLDQRIKQRIK 288
             V++ I NTL +VF  V PY +++ S+   WG+V ASDT  P  L+A ++D+ I  RI+
Sbjct: 207 PRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAASDTVDPAKLSAKEVDELIAARIR 266

Query: 289 GENKYFDGKTFSSASTLSKAVRKSLDNETHVY 318
           G+ +++DG+T      L   VR+ L +ET  Y
Sbjct: 267 GQLRFYDGETHEWMFRLPLPVRQVL-SETRDY 294

BLAST of Tan0017080 vs. ExPASy Swiss-Prot
Match: Q9UXE4 (Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=speE PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 112/276 (40.58%), Postives = 165/276 (59.78%), Query Frame = 0

Query: 46  LNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN-PK 105
           +  +L    + YQ + L++   FGK L+IDGK+QS   DE IYHE LVHP LL     PK
Sbjct: 21  IEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK 80

Query: 106 TIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFCDPRLELIIN 165
           ++ I+GGGEG+T RE+L++K+VEK VM DIDE+V++F K YL   ++ AF D R  L+I 
Sbjct: 81  SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVIT 140

Query: 166 DARAELENRMEQYDVIVGDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAG 225
           D    ++   E+YD I+ DL DPI+    Y LYTK FYE  ++  L + G  +TQA    
Sbjct: 141 DGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYE-KLREILNERGGIVTQATSPS 200

Query: 226 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQR 285
                EV+  IYNT+K+VFK       ++ SF   WG+V     P +L+ D++D +I +R
Sbjct: 201 F--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSKINER 260

Query: 286 IKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYTE 320
           I G+ +++DG +   + +L K ++      T + TE
Sbjct: 261 IDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTE 293

BLAST of Tan0017080 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 5.2e-54
Identity = 114/305 (37.38%), Postives = 186/305 (60.98%), Query Frame = 0

Query: 20  GHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQ 79
           G   + + K  W+ E +  +      +  ++  G S++Q+IA++     GK LV+DGK Q
Sbjct: 4   GSEKSVFLKWSWFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQ 63

Query: 80  SAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVV 139
           S+E+DEF+YHE LVHPA++ H +P+ + I+GGGEG+T RE+L+H++VEK VM DIDE VV
Sbjct: 64  SSESDEFMYHEALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVV 123

Query: 140 DFCKSYLIV-NKEAFCDPRLELIINDARAELENRMEQ-YDVIVGDLADPIEGGPCYKLYT 199
           +  + +L   ++ AF DPR E++I+DA   + ++ E  +DV++ DL DP+E GP  +LY+
Sbjct: 124 NVAREHLREWHRGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYS 183

Query: 200 KSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFAD 259
           + +Y   VK  +   G+F+TQA    I   TE  + I NT+ +VFK+V  Y  ++PSF  
Sbjct: 184 EEYYR-MVKDVMNPGGVFVTQA--VSISHLTEYHAIIRNTVARVFKHVESYGVYVPSFDS 243

Query: 260 TWGWVMASD--TPFVL-NADDLDQRIKQRIKG-ENKYFDGKTFSSASTLSKAVRKSLDNE 319
            WG+V+ASD   P +L +    + R+  +++G E ++ D  +      + K  R+++  E
Sbjct: 244 MWGFVVASDDKDPRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIARE 303

BLAST of Tan0017080 vs. NCBI nr
Match: XP_038880186.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 691.8 bits (1784), Expect = 2.9e-195
Identity = 333/338 (98.52%), Postives = 334/338 (98.82%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENR E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEY+VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
            LSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 338

BLAST of Tan0017080 vs. NCBI nr
Match: XP_022138299.1 (thermospermine synthase ACAULIS5-like [Momordica charantia])

HSP 1 Score: 690.6 bits (1781), Expect = 6.5e-195
Identity = 331/338 (97.93%), Postives = 335/338 (99.11%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEY+VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
           TLSKAVRKSLD ETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 338

BLAST of Tan0017080 vs. NCBI nr
Match: KAG6597450.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 688.3 bits (1775), Expect = 3.2e-194
Identity = 333/339 (98.23%), Postives = 336/339 (99.12%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENR EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEY+VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

BLAST of Tan0017080 vs. NCBI nr
Match: XP_023539341.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687.2 bits (1772), Expect = 7.2e-194
Identity = 332/339 (97.94%), Postives = 336/339 (99.12%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNP+TIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPRTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENR EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRSEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEY+VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

BLAST of Tan0017080 vs. NCBI nr
Match: XP_022934423.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 686.4 bits (1770), Expect = 1.2e-193
Identity = 332/339 (97.94%), Postives = 336/339 (99.12%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENR EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYE++VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEHTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

BLAST of Tan0017080 vs. ExPASy TrEMBL
Match: A0A6J1C9S2 (thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC111009509 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.2e-195
Identity = 331/338 (97.93%), Postives = 335/338 (99.11%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLEL+INDARAELE+R EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEY+VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
           TLSKAVRKSLD ETHVYTEGTARFIYGYGAAACKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAACKLNQV 338

BLAST of Tan0017080 vs. ExPASy TrEMBL
Match: A0A6J1F7M2 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441602 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.0e-194
Identity = 332/339 (97.94%), Postives = 336/339 (99.12%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENR EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYE++VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEHTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

BLAST of Tan0017080 vs. ExPASy TrEMBL
Match: A0A6J1IJL1 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111474176 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.3e-193
Identity = 332/339 (97.94%), Postives = 335/339 (98.82%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENR EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEY+VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIK ENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKEENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYG-AAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYG AAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

BLAST of Tan0017080 vs. ExPASy TrEMBL
Match: A0A0A0L7M9 (Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.5e-192
Identity = 329/338 (97.34%), Postives = 331/338 (97.93%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENR E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEY+VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
           TLSKAVRKSLDNET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

BLAST of Tan0017080 vs. ExPASy TrEMBL
Match: A0A5D3D0P3 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00350 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 3.3e-192
Identity = 328/338 (97.04%), Postives = 331/338 (97.93%), Query Frame = 0

Query: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENR E YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEY+VKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAACKLNQV 339
           TLSKAVRKSLDNET VYTE TARFIYGYGA ACKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEACKLNQV 338

BLAST of Tan0017080 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 463.8 bits (1192), Expect = 1.2e-130
Identity = 212/328 (64.63%), Postives = 268/328 (81.71%), Query Frame = 0

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 8   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 67

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 68  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 127

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E++D+IVGDL
Sbjct: 128 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 187

Query: 189 ADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 188 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 247

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 248 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 307

Query: 309 KAVRKSLDNETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 308 KTISLALEKETEVYSEENARFIHGHGVA 335

BLAST of Tan0017080 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 463.8 bits (1192), Expect = 1.2e-130
Identity = 212/328 (64.63%), Postives = 268/328 (81.71%), Query Frame = 0

Query: 9   LVNNGNELIEKGHS----LNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALL 68
           +  NG   I K  S    L+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALL
Sbjct: 1   MFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALL 60

Query: 69  DTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTARELLRH 128
           DTK FGK LVIDGK+QSAE DEFIYHECL+HPALL HPNPKT+FIMGGGEGS ARE+L+H
Sbjct: 61  DTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKH 120

Query: 129 KTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRMEQYDVIVGDL 188
            T+EKVVMCDID+EVVDFC+ +L VN +AFC+ +LEL+I DA+AELE R E++D+IVGDL
Sbjct: 121 TTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDL 180

Query: 189 ADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFK 248
           ADP+EGGPCY+LYTKSFY+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFK
Sbjct: 181 ADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFK 240

Query: 249 YVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLS 308
           YV  Y+AH+PSFADTWGWVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+
Sbjct: 241 YVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLN 300

Query: 309 KAVRKSLDNETHVYTEGTARFIYGYGAA 332
           K +  +L+ ET VY+E  ARFI+G+G A
Sbjct: 301 KTISLALEKETEVYSEENARFIHGHGVA 328

BLAST of Tan0017080 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 157.1 bits (396), Expect = 2.4e-38
Identity = 81/225 (36.00%), Postives = 128/225 (56.89%), Query Frame = 0

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ L+I
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELENRME-QYDVIVGDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F++ SV   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSFADTWG-WVMA 265
             ++ H ++   I +  +++FK  V Y+   +P++   WG W+ A
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

BLAST of Tan0017080 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 157.1 bits (396), Expect = 2.4e-38
Identity = 88/280 (31.43%), Postives = 153/280 (54.64%), Query Frame = 0

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ L+I
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELENRME-QYDVIVGDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F++ SV   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMAS----DTPFVLNADD 282
             ++ H ++   I +  +++FK  V Y+   +P++ +   G+++ S    D  F    + 
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNP 344

Query: 283 LDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETH 316
           +D+    +  G  K+++ +  S+A  L    +K ++++ +
Sbjct: 345 IDES-SSKSNGPLKFYNAEIHSAAFCLPSFAKKVIESKAN 378

BLAST of Tan0017080 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 153.3 bits (386), Expect = 3.5e-37
Identity = 94/283 (33.22%), Postives = 151/283 (53.36%), Query Frame = 0

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPN 102
           S  +  IL  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELII 162
           PK + ++GGG+G   RE+ RH +VE++ +C+ID+ VVD  K Y       + DPR+ LII
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 163 NDARAELENRME-QYDVIVGDLADPIEGGPCYKLYTKSFYEYSVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F+E SV   L+  G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFE-SVNRALRPGGVVCTQA-- 244

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMAS------DTPFVLNA 282
             ++ H ++   I +  + +FK  V Y+   +P++ +   G+++ S      D    ++ 
Sbjct: 245 ESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFKKPVSL 304

Query: 283 DDLDQ-RIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETH 316
            D D+  IK       KY++ +  S+A  L    +K +D++ +
Sbjct: 305 IDTDESSIKSHC--PLKYYNAEIHSAAFCLPSFAKKVIDSKAN 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7X61.7e-12964.63Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... [more]
Q975S54.6e-5843.32Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ... [more]
A2BJU21.9e-5644.12Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / ... [more]
Q9UXE41.8e-5440.58Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 3509... [more]
Q9YE025.2e-5437.38Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1... [more]
Match NameE-valueIdentityDescription
XP_038880186.12.9e-19598.52thermospermine synthase ACAULIS5-like [Benincasa hispida][more]
XP_022138299.16.5e-19597.93thermospermine synthase ACAULIS5-like [Momordica charantia][more]
KAG6597450.13.2e-19498.23Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023539341.17.2e-19497.94thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo][more]
XP_022934423.11.2e-19397.94thermospermine synthase ACAULIS5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1C9S23.2e-19597.93thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6J1F7M26.0e-19497.94thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441... [more]
A0A6J1IJL11.3e-19397.94thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11147417... [more]
A0A0A0L7M91.5e-19297.34Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1[more]
A0A5D3D0P33.3e-19297.04Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
AT5G19530.11.2e-13064.63S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G19530.21.2e-13064.63S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23820.22.4e-3836.00spermidine synthase 1 [more]
AT1G23820.12.4e-3831.43spermidine synthase 1 [more]
AT1G70310.13.5e-3733.22spermidine synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 82..334
e-value: 3.6E-77
score: 261.3
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 85..262
e-value: 4.3E-42
score: 143.6
NoneNo IPR availablePANTHERPTHR43317THERMOSPERMINE SYNTHASE ACAULIS5coord: 1..331
NoneNo IPR availablePANTHERPTHR43317:SF9SPERMINE SYNTHASEcoord: 1..331
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 110..218
e-value: 2.61312E-7
score: 46.6543
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 29..81
e-value: 7.1E-15
score: 56.8
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 31..82
e-value: 2.5E-13
score: 49.7
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 29..266
score: 96.876915
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 30..311
score: 31.074093
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 29..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0017080.1Tan0017080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006596 polyamine biosynthetic process
molecular_function GO:0010487 thermospermine synthase activity
molecular_function GO:0003824 catalytic activity