Lcy04g007110 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g007110
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionSec14p-like phosphatidylinositol transfer family protein
LocationChr04: 37283930 .. 37287900 (-)
RNA-Seq ExpressionLcy04g007110
SyntenyLcy04g007110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCACTAAAAAGCCGAGAGGTAATGCAACGGAGAAGGTTTTGTCGTCTGAAGAACAGCAGGCCAAGGTGAGGTTGCAGGTTTAATTTGTTAAAAGTATTTTAATAGAACAAGAAAATTGGATCTCTCTTATTCCCAATTTGGTACATGGTGTAAATGTGCTATAAACCTAAAAATGATTTCAAAAGGAGATGCCACCTAAGATATTTTTAGGGCATGTTTTGGAGGGATTATTCAAAGTGATTTTAACCGATTCAAAATCGCTCCCAAACATGTAGTTTTCTAGTAATGATTTGTGGGGTTTAATATTAAGTGTATTCTCTTTTTCAGATAAATGAAGTAAAAAAATTGATGGGTCCAATTGCTGATAAGCTTCCTGTTTTGTGCTCGGATGCATCAATTCTGAGGTACCTCAAAGCAAGAAACTGGAATACAAAGAAGGCCAATAAAATGTTGAAGGAAACTTTGAAATGGAGGCTAGAATACAAGCCAGAGAAGATAAGATGGGTATGGTTTTCTGCAAACTTCACCATTTAATTGGCACAATTCTAAATTTTAATCTCTCAGGAAAATGGAAAATGGGGCTAAATACTGAACTGAGGGGGAAGAAAAGCACTTAATTTCTAGAATGGGCATAAACTTTAGAAGATAGAGTTGCATCAAATGGAGGATGCTAACAAGCATTGAGATTAGCCTTTTTTGATTTAAAGAAAAAAAATCGTTTCAAAAAATGATTTAAAGTAACAAGTAATAACAAAAATATTACAGAGTACATACCAGCAGTAATAACAAAAAGAAGTAACCATTTTCTCTGTATTACAATGTTTTCAAACTTACCTTCAAATGAGATTTTCTTTGCTTCATTATGATCTTGATTCTATGTGAAGAATTCTCATTCCATTGAGAATATAATTTGCTGATTATAAGGCATGAGAGATTCAAGATTGTATAAATATAATATGCACTCCTCCATTCTTTTTAACTTTTTGAATGTAGAACAACTGGAAGTGGAAACTTTCTCTGCTAAAATGATTTTAGTTATGATATTATCTATATTTCTTCCCCTTCCCTTCGGACAACAGACACTTCGTATATCTTTACATTGGTATGATATTGTCCACTTTGGACATAAGCCCTCATGGCTTTGCTTTTGGTTCCATCCAAAAGGCCTCATACTAATGGAAATACTTGTCTTCTCTTATATACCCATGATCATCCCCTTGTCTAGCCAATGTGGAACTTTGGTCGCACTCCCAACAGGAACAAGCAAATCTCTAAAGCTAATTTTAAGAATGTGAAATTGTAGTAGTAAATTTTACTTGATTATGGTAGGTCACTGTTTTTTTAATTTTTGCTTGAAAGGACCGTATTGATCTTAGGACTGGATCCTATTGTATGCGTTTTTTACTAAACAATGTCTTGTTCGTGCTTCAAGGAAGACATTGCTCTGGAAGCCAAGACTGGAAAAATATATAGAGCTAATTACACTGACAAACAAGGAAGGACCGTTCTGGTAATGACACCAAGTTGTCAGGTATTTCAATTGCACGACAAACACACGTTACGTTCTTTAGTAGTTTCTACTTGCCTCTTTTATGTTGTGTTATATTAAACATATTCAACAAATGACTACAATCTTACTTATATCATATATGTTTTCGGTTAAATTATTAGTTCTGAAATTCAACTCTTAAAATTGTTATTTTAGCATATGTAATTTAAAAACCTTCTAATAGATGACCAAACTTATAAGGACATACCAAATTAATCCTATACTTCAATAATATTTTAATAGGTTATGTTATTGAACTTTCATTTTCGAGTGTAACAGATTCATATCATGAACTTCATTAAGTTATGCTTATGTCACCCAAATATCTAGGCTTGCTTGAAATTAGCCCTTCATACCTAATCCTTAAAGTGCATCAGATTATTGAAATCTACGGTGTAAATGTGTAATCTATTAGATACAAAAGTAAGAGTCGAAGACATATCAAAAGTTTTAAAATGCAAAAACAAATATACATAACCTTCAGATTTTAGGAATCTTTAAGAAACCTTTATATACTAATTGAACGTTGACTAAATAGACTCAAACTTCAATGAGAATACAGAGTTGTAAAGTATGAGTGAGAACTCTTCCTTGACGTGTTCGTTTTTTTTTCCTTACATAACTAATCTTGGTAAAGTACAATCGACTTTTCTTATAACAATGCCAGAATACAGAATCAACAAGTGGACAGATCCGATACTTAGTTTACTGCATGGAGAATGCGATCTTGAGTTCAAATTCAAATGATGGATACATGGTGTGGCTAATTGATTTTCATGGGTGGAGCACATCTTGTTTGTCCATGAAGGTTACTCGAGAAACAGCTAACGTCCTGCAAAATCATTATCCTGAAAGATTGGGCCTTGCTATTCTCTACAACCCACCGAAGGTTTTTGAGTCCTTTTGGGTGGTAAGCTATCATATCACTTCCATATTCTGTTGAATTATTGCCTTCACATTGATTGCTTTTCATATGTTATGAAATTTCCTAAAAAGTTTGGGTTCCCCACTGAGATGAATTTGTATGCGCCTAATCTTATCTATCCTTCTAAATCACCATGCATCTCATCTCCTCTGGACAGTTGAATTAAGATTTTTAAGGTCCCATTTGATAATCATTTTATTTTTGGTTTTTGAAAATTAAGCTTATAAACACTAGCTCCACCTATAAGTTTCTTTGTTTTCTTATCGATCTTCTTATCATATCTTCGAAAACCAAGCCAAGTTTTAAAAACTAAAAAAATTAGGTCCCGTTTGATAACCATTTGGTTTTTGGTTTTTGGTTTTTGAAAATTAAGCTTATACACACCTACTTCCACCTGTAACTTTCTTTGTTTTAGTATCAATCTTCTTCCTTGTTTTAAAAAACCAAGTCCAATTTTGAAAACAAAAAAAAAAAGTTTTCAAAAACTTGTTTTTGTTTTTAGAATTTGGCTAGGAATTCAAATGGATCTTTAACAAGAATGAAAGTTATGGTAGGAAATTTAGGAGAAAACAAGCATATTTTTCAAAAATAAAAAACAAAAAACCAAATGGTTATCAAACGGGGTCTTAGTTTTCAAAAACTTGTTTTTGTTTTTAGAATTTGGCTAAGAATTTAACAAAGATGAAAACCATTGCAAGCAAATTTGGAAAAAACAAGCATAATTTTAAAAAACCAAAAACAAAAAACCAAATGGTTATTAAACGAGACCTTAATCTTTTTACTTCACCTATTTATTAGTGTCGTAGCATCTTATAACGAAATGGACGTTTTGAAAGGTTTAACCAAAACGAACCACATGGATGACAATAATACAACTTTTAAAATCACATGAGAAAATGAAATCAAGCTGAAACTATGTTGGCACATTTGCCTGTTCTGACGTGTGTCTTGCTTGAGATAATGCAGATGGTAAGACCATTCCTAGAGTCTAAGACATACAAGAAAGTTAAGTTTGTGTACTCCAACAACCCAGAGAGCCAGAAGATAATGGAAGAGAACTTCGACAGAGACAAGTTGGAAACTTCATTTGGTGGGAAAAATCCAGTTGGGTTTAACTACGAAGCTTATGCACAAAGAATGATGGAAGATGACAAGAAAATGACTGATTTTATTGACTCTGGATGCTCTTCTCCAAATTACAGAGCTATTTTATCCAAATCACAGCCGCCGGACTCGGCAGCGTCCGTTGTCGACTCTCAGGCTTCCGATGAAGATATCGACCACAAAATCTCTTTAAATTTGGAGCTTCCAAATGAAAAATTGCGAGAAAATTGATTCGCGTAAGTTGCTTGTCACAGGGAAAATTTCTAGTACTATGGTAGTGTGGAGAAATTTTGGTACTACCTCCATTCATTATTTGTCATGAAAGAAGCATTCAAGAATGTGGAAGACCTATGGAATAAAATATTATATCATTAAATGACGTGACAAAT

mRNA sequence

ATGTCCACTAAAAAGCCGAGAGGTAATGCAACGGAGAAGGTTTTGTCGTCTGAAGAACAGCAGGCCAAGATAAATGAAGTAAAAAAATTGATGGGTCCAATTGCTGATAAGCTTCCTGTTTTGTGCTCGGATGCATCAATTCTGAGGTACCTCAAAGCAAGAAACTGGAATACAAAGAAGGCCAATAAAATGTTGAAGGAAACTTTGAAATGGAGGCTAGAATACAAGCCAGAGAAGATAAGATGGGAAGACATTGCTCTGGAAGCCAAGACTGGAAAAATATATAGAGCTAATTACACTGACAAACAAGGAAGGACCGTTCTGGTAATGACACCAAGTTGTCAGAATACAGAATCAACAAGTGGACAGATCCGATACTTAGTTTACTGCATGGAGAATGCGATCTTGAGTTCAAATTCAAATGATGGATACATGGTGTGGCTAATTGATTTTCATGGGTGGAGCACATCTTGTTTGTCCATGAAGGTTACTCGAGAAACAGCTAACGTCCTGCAAAATCATTATCCTGAAAGATTGGGCCTTGCTATTCTCTACAACCCACCGAAGGTTTTTGAGTCCTTTTGGGTGATGGTAAGACCATTCCTAGAGTCTAAGACATACAAGAAAGTTAAGTTTGTGTACTCCAACAACCCAGAGAGCCAGAAGATAATGGAAGAGAACTTCGACAGAGACAAGTTGGAAACTTCATTTGGTGGGAAAAATCCAGTTGGGTTTAACTACGAAGCTTATGCACAAAGAATGATGGAAGATGACAAGAAAATGACTGATTTTATTGACTCTGGATGCTCTTCTCCAAATTACAGAGCTATTTTATCCAAATCACAGCCGCCGGACTCGGCAGCGTCCGTTGTCGACTCTCAGGCTTCCGATGAAGATATCGACCACAAAATCTCTTTAAATTTGGAGCTTCCAAATGAAAAATTGCGAGAAAATTGATTCGCGTAAGTTGCTTGTCACAGGGAAAATTTCTAGTACTATGGTAGTGTGGAGAAATTTTGGTACTACCTCCATTCATTATTTGTCATGAAAGAAGCATTCAAGAATGTGGAAGACCTATGGAATAAAATATTATATCATTAAATGACGTGACAAAT

Coding sequence (CDS)

ATGTCCACTAAAAAGCCGAGAGGTAATGCAACGGAGAAGGTTTTGTCGTCTGAAGAACAGCAGGCCAAGATAAATGAAGTAAAAAAATTGATGGGTCCAATTGCTGATAAGCTTCCTGTTTTGTGCTCGGATGCATCAATTCTGAGGTACCTCAAAGCAAGAAACTGGAATACAAAGAAGGCCAATAAAATGTTGAAGGAAACTTTGAAATGGAGGCTAGAATACAAGCCAGAGAAGATAAGATGGGAAGACATTGCTCTGGAAGCCAAGACTGGAAAAATATATAGAGCTAATTACACTGACAAACAAGGAAGGACCGTTCTGGTAATGACACCAAGTTGTCAGAATACAGAATCAACAAGTGGACAGATCCGATACTTAGTTTACTGCATGGAGAATGCGATCTTGAGTTCAAATTCAAATGATGGATACATGGTGTGGCTAATTGATTTTCATGGGTGGAGCACATCTTGTTTGTCCATGAAGGTTACTCGAGAAACAGCTAACGTCCTGCAAAATCATTATCCTGAAAGATTGGGCCTTGCTATTCTCTACAACCCACCGAAGGTTTTTGAGTCCTTTTGGGTGATGGTAAGACCATTCCTAGAGTCTAAGACATACAAGAAAGTTAAGTTTGTGTACTCCAACAACCCAGAGAGCCAGAAGATAATGGAAGAGAACTTCGACAGAGACAAGTTGGAAACTTCATTTGGTGGGAAAAATCCAGTTGGGTTTAACTACGAAGCTTATGCACAAAGAATGATGGAAGATGACAAGAAAATGACTGATTTTATTGACTCTGGATGCTCTTCTCCAAATTACAGAGCTATTTTATCCAAATCACAGCCGCCGGACTCGGCAGCGTCCGTTGTCGACTCTCAGGCTTCCGATGAAGATATCGACCACAAAATCTCTTTAAATTTGGAGCTTCCAAATGAAAAATTGCGAGAAAATTGA

Protein sequence

MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKKANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTESTSGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLGLAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGKNPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDIDHKISLNLELPNEKLREN
Homology
BLAST of Lcy04g007110 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.3e-25
Identity = 69/243 (28.40%), Postives = 124/243 (51.03%), Query Frame = 0

Query: 17  SEEQQAKINEVKKLMGPIADKLPV-LCSDASILRYLKARNWNTKKANKMLKETLKWRLEY 76
           +E+Q     E+K     + D   +  C+D   LRYL+ARN+   K+ KML++TL+WR ++
Sbjct: 45  NEKQLEAFKEIKSNFSDLTDPTDIAFCTDMCFLRYLRARNYIVSKSEKMLRDTLEWRKKF 104

Query: 77  KPEKIR-WEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTESTSGQIRY--LVYCME 136
           +P+ I+   DI      G +Y  N  DK+GR ++   P     ++   ++++  LVY +E
Sbjct: 105 RPQDIQLGGDIREIGSAGCVY-VNKRDKKGRPIIFAVPRNDTLKNVPSELKFKNLVYWLE 164

Query: 137 NAILSSNSNDG--YMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLGLAILYNPPKV 196
                 +   G     +++D+  + +  + MK   E  + L +H PER+G ++  +PP +
Sbjct: 165 QGFSRMDEPKGIEQFCFIVDYKDFGSGNMDMKTNLEAMHFLLDHCPERMGQSLFLDPPAL 224

Query: 197 FESFWVMVRPFLESKTYKKVKFVYSNNPESQKI---MEENFDRDKLETSFGGKNPVGFNY 251
           F   W ++ PFL   T  KV+F+ S   + ++    + E  D + LE + GG     +N 
Sbjct: 225 FWFAWKIISPFLNEVTLSKVRFINSKKVDGKRTFAELLEYVDIENLEQNLGGNLDYNYNI 284

BLAST of Lcy04g007110 vs. ExPASy Swiss-Prot
Match: Q9UU99 (CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC23B6.04c PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.5e-23
Identity = 72/211 (34.12%), Postives = 115/211 (54.50%), Query Frame = 0

Query: 47  ILRYLKARNWNTKKANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRT 106
           ILRYL+A  W+   A K + +TL WR  +    +  ++I  E  TGK     Y DK GR 
Sbjct: 638 ILRYLRATKWHVSNAKKRIVDTLVWRRHFGVNNMDPDEIQEENATGKQVLLGY-DKDGRP 697

Query: 107 VLVMTPSCQNTESTSGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWST-SCLSMKVTR 166
            L + P+ QNT+++  QIR+LV+ +E AI         +  LI+F   S  S  S+   +
Sbjct: 698 CLYLYPARQNTKTSPLQIRHLVFSLECAIDLMPPGVETLALLINFKSSSNRSNPSVGQGK 757

Query: 167 ETANVLQNHYPERLGLAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIME 226
           E  N+LQ HY ERLG A++ N P     F+ ++ PF++  T +K+KF   N P     ++
Sbjct: 758 EVLNILQTHYCERLGRALVINIPWAVWGFFKLISPFIDPITREKLKF---NEP-----LD 817

Query: 227 ENFDRDKLETSFGGKNPVGFNYEAYAQRMME 257
               +D+L+++FGG     +++E Y  +++E
Sbjct: 818 RYVPKDQLDSNFGGSLHFEYHHEKYWPQLVE 839

BLAST of Lcy04g007110 vs. ExPASy Swiss-Prot
Match: P53844 (Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR17 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.7e-18
Identity = 74/247 (29.96%), Postives = 114/247 (46.15%), Query Frame = 0

Query: 33  PIADKLPVLCSDASILRYLKARNWNTKKANKMLKETLKWRLEY-----KPEK--IRWEDI 92
           P++D      S    LRYL+A  WNT  A K L +TL WR E      K +K  +  + +
Sbjct: 81  PLSDWEKFWLSRECFLRYLRANKWNTANAIKGLTKTLVWRREIGLTHGKEDKDPLTADKV 140

Query: 93  ALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTESTSGQIRYLVYCMENAILSSNSNDGYM 152
           A+E +TGK     + D   R +  M    QNTES+  Q++ LVY ME A   +      +
Sbjct: 141 AVENETGKQVILGF-DNAKRPLYYMKNGRQNTESSFRQVQELVYMMETATTVAPQGVEKI 200

Query: 153 VWLIDFHGW------STSCLSMKVTRETANVLQNHYPERLGLAILYNPPKVFESFWVMVR 212
             L+DF  +      +     + + R   NV+Q+HYPERL   +L N P    +F  M+ 
Sbjct: 201 TVLVDFKSYKEPGIITDKAPPISIARMCLNVMQDHYPERLAKCVLINIPWFAWAFLKMMY 260

Query: 213 PFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGKNPVGFNYEAYAQRMME--D 265
           PFL+  T  K  F         +  E + +  +L+  + G     + +E Y   M++  D
Sbjct: 261 PFLDPATKAKAIF--------DEPFENHIEPSQLDALYNGLLDFKYKHEVYWPDMVKKVD 318

BLAST of Lcy04g007110 vs. ExPASy Swiss-Prot
Match: P53860 (Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR16 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.4e-18
Identity = 69/271 (25.46%), Postives = 131/271 (48.34%), Query Frame = 0

Query: 9   NATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKKANKMLKET 68
           N   KV +SE+     N+ +  + P+ ++     +    LRYL+A  W  K     +  T
Sbjct: 57  NPDLKVYTSEK-----NKSEDDLKPLEEEEKAWLTRECFLRYLRATKWVLKDCIDRITMT 116

Query: 69  LKWRLEY--------KPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 128
           L WR E+          +KI  + +A+E ++GK     Y +   R +L + P  QNT+++
Sbjct: 117 LAWRREFGISHLGEEHGDKITADLVAVENESGKQVILGY-ENDARPILYLKPGRQNTKTS 176

Query: 129 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGW--------STSCLSMKVTRETANVLQ 188
             Q+++LV+ +E  I    +    +  LIDF  +        ++    + V +E  ++LQ
Sbjct: 177 HRQVQHLVFMLERVIDFMPAGQDSLALLIDFKDYPDVPKVPGNSKIPPIGVGKEVLHILQ 236

Query: 189 NHYPERLGLAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDK 248
            HYPERLG A+L N P +  +F  ++ PF++  T +K+ F         +   +   +++
Sbjct: 237 THYPERLGKALLTNIPWLAWTFLKLIHPFIDPLTREKLVF--------DEPFVKYVPKNE 296

Query: 249 LETSFGGKNPVGFNYEAYAQRMMEDDKKMTD 264
           L++ +GG     +N++ Y   ++E  ++  D
Sbjct: 297 LDSLYGGDLKFKYNHDVYWPALVETAREKRD 313

BLAST of Lcy04g007110 vs. ExPASy Swiss-Prot
Match: P24859 (SEC14 cytosolic factor OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 2.5e-13
Identity = 69/245 (28.16%), Postives = 119/245 (48.57%), Query Frame = 0

Query: 19  EQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKKANKMLKETLKWRLEYKPE 78
           EQ+AK+ E ++L+  +  K  +   D+++LR+L+AR ++ + +  M +   KWR E+  +
Sbjct: 30  EQEAKLKEFRELLESLGYKERL--DDSTLLRFLRARKFDLEASKIMYENCEKWRKEFGVD 89

Query: 79  KIRWEDIALEAK--TGKIYRANY--TDKQGRTVLVMTPSCQN------TESTSGQIRYLV 138
            I +ED   E K    K Y   Y  TD  GR V +      N        +    ++ LV
Sbjct: 90  TI-FEDFHYEEKPLVAKYYPQYYHKTDNDGRPVYIEELGSVNLTQMYKITTQERMLKNLV 149

Query: 139 YCMENAIL----SSNSNDGYMV----WLIDFHGWSTSCLS--MKVTRETANVLQNHYPER 198
           +  E  +     + +   GY+V     ++D  G S S  +  +   RE +N+ QN+YPER
Sbjct: 150 WEYEAFVRYRLPACSRKAGYLVETSCTILDLKGISISSAAQVLSYVREASNIGQNYYPER 209

Query: 199 LGLAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFG 244
           +G   L N P  F + + + +PFL+  T  K+  + S+    QK + +    + L   FG
Sbjct: 210 MGKFYLINAPFGFSTAFRLFKPFLDPVTVSKIFILGSS---YQKDLLKQIPAENLPKKFG 268

BLAST of Lcy04g007110 vs. ExPASy TrEMBL
Match: A0A0A0L1I2 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G594430 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 7.2e-149
Identity = 271/318 (85.22%), Postives = 289/318 (90.88%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +K R NATEKV S EEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK
Sbjct: 1   MSARKSRANATEKVWSHEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           ANKMLKETLKWRLEYKPEKI WEDIA EAKTGKIYRA YTDKQGRTVLVM PS QNTEST
Sbjct: 61  ANKMLKETLKWRLEYKPEKISWEDIAQEAKTGKIYRATYTDKQGRTVLVMRPSSQNTEST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
           +GQIRYLVYCMENAILSSNS DGYMVWLIDFHGW+TSCLSMKVTR+TA+VLQNHYPERLG
Sbjct: 121 TGQIRYLVYCMENAILSSNSTDGYMVWLIDFHGWNTSCLSMKVTRDTAHVLQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPK+FESFWVMVRPFLESKT KKVKFVYSNNPES KIMEENFD DKLE+SFGG+
Sbjct: 181 LAILYNPPKLFESFWVMVRPFLESKTSKKVKFVYSNNPESLKIMEENFDADKLESSFGGR 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDI 300
           NP+GFNYE Y+QRMMEDDKKMT FIDS CSSP Y+A+LSKSQ  DSAA  VDSQASD++ 
Sbjct: 241 NPIGFNYEDYSQRMMEDDKKMTHFIDSRCSSPTYKALLSKSQMLDSAAFDVDSQASDDES 300

Query: 301 D-HKISLNLELPNEKLRE 318
           D  +I  NL  P++KL+E
Sbjct: 301 DTDEIPSNLRRPDDKLQE 318

BLAST of Lcy04g007110 vs. ExPASy TrEMBL
Match: A0A1S3B5J6 (random slug protein 5-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486028 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.2e-148
Identity = 269/318 (84.59%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +K R NATEKVLS++EQQA+INEVKK MGPIA KLPVLCSDASILRYLKARNWNTKK
Sbjct: 1   MSARKSRANATEKVLSNQEQQARINEVKKFMGPIAHKLPVLCSDASILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           ANKMLKETLKWRLEYKPEKI WEDIA EAKTGKIYRANYTD+QGRTVLVM PS QNTEST
Sbjct: 61  ANKMLKETLKWRLEYKPEKISWEDIAQEAKTGKIYRANYTDRQGRTVLVMRPSSQNTEST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
           +GQIRYLVYCME+AILSSNS DGYMVWLIDFHGW+TSCLS+KVTR+TA+VLQNHYPERLG
Sbjct: 121 TGQIRYLVYCMEHAILSSNSTDGYMVWLIDFHGWNTSCLSLKVTRDTAHVLQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPKVFESFWVMVRPFLESKT KKVKFVYSNNPES KIM +NFD DKLETSFGGK
Sbjct: 181 LAILYNPPKVFESFWVMVRPFLESKTSKKVKFVYSNNPESLKIMADNFDADKLETSFGGK 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDI 300
           NP+GFNYE Y+QRMMEDDKKMT FIDSGCSSP Y+A+LSKSQ  DSAAS  DSQASD++ 
Sbjct: 241 NPIGFNYEDYSQRMMEDDKKMTRFIDSGCSSPTYKALLSKSQLLDSAASDFDSQASDDES 300

Query: 301 D-HKISLNLELPNEKLRE 318
           D  +I  NL LP++KL+E
Sbjct: 301 DTDEIPSNLGLPDDKLQE 318

BLAST of Lcy04g007110 vs. ExPASy TrEMBL
Match: A0A5D3C075 (Random slug protein 5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G00440 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.2e-148
Identity = 269/318 (84.59%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +K R NATEKVLS++EQQA+INEVKK MGPIA KLPVLCSDASILRYLKARNWNTKK
Sbjct: 1   MSARKSRANATEKVLSNQEQQARINEVKKFMGPIAHKLPVLCSDASILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           ANKMLKETLKWRLEYKPEKI WEDIA EAKTGKIYRANYTD+QGRTVLVM PS QNTEST
Sbjct: 61  ANKMLKETLKWRLEYKPEKISWEDIAQEAKTGKIYRANYTDRQGRTVLVMRPSSQNTEST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
           +GQIRYLVYCME+AILSSNS DGYMVWLIDFHGW+TSCLS+KVTR+TA+VLQNHYPERLG
Sbjct: 121 TGQIRYLVYCMEHAILSSNSTDGYMVWLIDFHGWNTSCLSLKVTRDTAHVLQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPKVFESFWVMVRPFLESKT KKVKFVYSNNPES KIM +NFD DKLETSFGGK
Sbjct: 181 LAILYNPPKVFESFWVMVRPFLESKTSKKVKFVYSNNPESLKIMADNFDADKLETSFGGK 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDI 300
           NP+GFNYE Y+QRMMEDDKKMT FIDSGCSSP Y+A+LSKSQ  DSAAS  DSQASD++ 
Sbjct: 241 NPIGFNYEDYSQRMMEDDKKMTRFIDSGCSSPTYKALLSKSQLLDSAASDFDSQASDDES 300

Query: 301 D-HKISLNLELPNEKLRE 318
           D  +I  NL LP++KL+E
Sbjct: 301 DTDEIPSNLGLPDDKLQE 318

BLAST of Lcy04g007110 vs. ExPASy TrEMBL
Match: A0A6J1DKE0 (random slug protein 5-like OS=Momordica charantia OX=3673 GN=LOC111021741 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.6e-146
Identity = 270/316 (85.44%), Postives = 288/316 (91.14%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +KPRGNATEKVLS EEQQAKI+EVKKLMGPIADKLPVLCS+ SILRYLKARNWNTKK
Sbjct: 1   MSFRKPRGNATEKVLSPEEQQAKIDEVKKLMGPIADKLPVLCSNPSILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           A+KMLKETLKWRLEYKPEKI WEDIA EAKTGKIY+ANYTDKQGRTVLVMTPS QNT+ST
Sbjct: 61  ASKMLKETLKWRLEYKPEKISWEDIADEAKTGKIYKANYTDKQGRTVLVMTPSNQNTDST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
            GQIRYLVY MENAILSSNSNDGYMVWLIDFHGW+TS LS+K TRETA VLQNHYPERLG
Sbjct: 121 IGQIRYLVYSMENAILSSNSNDGYMVWLIDFHGWNTSSLSVKTTRETAYVLQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFD +KLETSFGGK
Sbjct: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDEEKLETSFGGK 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDS-QASD-E 300
           +P+GFNYE YA+RMMEDDKKMT FID+GCSSP YR ILS SQ PDSAAS  DS +ASD E
Sbjct: 241 SPIGFNYEDYAKRMMEDDKKMTHFIDTGCSSPIYRTILSTSQLPDSAASDADSDEASDHE 300

Query: 301 DIDHKISLNLELPNEK 315
             D +I  NL+ P++K
Sbjct: 301 HSDDEIPSNLKTPDDK 316

BLAST of Lcy04g007110 vs. ExPASy TrEMBL
Match: A0A6J1GFS4 (phosphatidylinositol transfer protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111453549 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.3e-146
Identity = 260/318 (81.76%), Postives = 293/318 (92.14%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +K RGN +EKVLSSEEQQAKINEVKKLMGP+ +KLPVLCSDASILRYLKARNWNTKK
Sbjct: 1   MSARKSRGNGSEKVLSSEEQQAKINEVKKLMGPLTEKLPVLCSDASILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           ANKML+ETLKW+LEYKPEKI WEDIALEAKTGKIYRANYTDKQGRTVLVM PS QNTEST
Sbjct: 61  ANKMLRETLKWKLEYKPEKISWEDIALEAKTGKIYRANYTDKQGRTVLVMRPSSQNTEST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
           +GQIRYLVYCMENAIL++NS DGYMVWLIDFHGW+TSCLS+KVTR+TAN+LQNHYPERLG
Sbjct: 121 TGQIRYLVYCMENAILNTNSTDGYMVWLIDFHGWNTSCLSLKVTRDTANILQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPKVFESFWVMVRPFLESKT+KKVKFVYSNNPESQKIME+NF+ ++LETSFGGK
Sbjct: 181 LAILYNPPKVFESFWVMVRPFLESKTFKKVKFVYSNNPESQKIMEDNFNVEELETSFGGK 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDI 300
           NPVGFNYE YA+RM+E DKK T+F+DSG SSP  ++ILSKSQ PDSA S  +SQ SD++ 
Sbjct: 241 NPVGFNYEDYARRMVEGDKKKTEFVDSGFSSPTSKSILSKSQLPDSAVSDANSQDSDDES 300

Query: 301 DHKIS-LNLELPNEKLRE 318
           D+++   NL LP++KL+E
Sbjct: 301 DNEMQPSNLALPDDKLQE 318

BLAST of Lcy04g007110 vs. NCBI nr
Match: XP_038881392.1 (phosphatidylinositol transfer protein 3-like [Benincasa hispida])

HSP 1 Score: 543.5 bits (1399), Expect = 1.2e-150
Identity = 273/317 (86.12%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +K RGNATEKVLS EEQQ KINEVKKLMGPIADKLPVLCS+ASILRYLKARNWNTKK
Sbjct: 1   MSARKSRGNATEKVLSHEEQQVKINEVKKLMGPIADKLPVLCSEASILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           ANKMLKETLKWRL+YKPEKI WEDIA EAKTGKIYRANYTDKQGRTVLVM PS QNTEST
Sbjct: 61  ANKMLKETLKWRLDYKPEKIIWEDIAQEAKTGKIYRANYTDKQGRTVLVMRPSSQNTEST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
           +GQIRYLVYCME+AILSSNS DGYMVWLIDFHGW+TSCLS+KVTR+TA+VLQNHYPERLG
Sbjct: 121 TGQIRYLVYCMEHAILSSNSTDGYMVWLIDFHGWNTSCLSLKVTRDTAHVLQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPKVFESFWVMVRPFLESKT+KKVKFVYSNNPES+KIME+NFD D+LETSFGGK
Sbjct: 181 LAILYNPPKVFESFWVMVRPFLESKTFKKVKFVYSNNPESRKIMEDNFDADELETSFGGK 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDI 300
           NPVGFNYE Y+QRMMEDDKKMT FIDSGCSSP Y+AILSKSQ  DSAAS VDSQ SDE+ 
Sbjct: 241 NPVGFNYEDYSQRMMEDDKKMTHFIDSGCSSPIYKAILSKSQLLDSAASDVDSQTSDEES 300

Query: 301 DH-KISLNLELPNEKLR 317
           ++ +I L+LE P++KL+
Sbjct: 301 NNDEIPLSLEHPDDKLQ 317

BLAST of Lcy04g007110 vs. NCBI nr
Match: XP_011653957.1 (phosphatidylinositol transfer protein 3 isoform X1 [Cucumis sativus] >XP_031739558.1 phosphatidylinositol transfer protein 3 isoform X1 [Cucumis sativus] >XP_031739559.1 phosphatidylinositol transfer protein 3 isoform X1 [Cucumis sativus] >XP_031739560.1 phosphatidylinositol transfer protein 3 isoform X1 [Cucumis sativus] >KGN54909.1 hypothetical protein Csa_012764 [Cucumis sativus])

HSP 1 Score: 536.6 bits (1381), Expect = 1.5e-148
Identity = 271/318 (85.22%), Postives = 289/318 (90.88%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +K R NATEKV S EEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK
Sbjct: 1   MSARKSRANATEKVWSHEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           ANKMLKETLKWRLEYKPEKI WEDIA EAKTGKIYRA YTDKQGRTVLVM PS QNTEST
Sbjct: 61  ANKMLKETLKWRLEYKPEKISWEDIAQEAKTGKIYRATYTDKQGRTVLVMRPSSQNTEST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
           +GQIRYLVYCMENAILSSNS DGYMVWLIDFHGW+TSCLSMKVTR+TA+VLQNHYPERLG
Sbjct: 121 TGQIRYLVYCMENAILSSNSTDGYMVWLIDFHGWNTSCLSMKVTRDTAHVLQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPK+FESFWVMVRPFLESKT KKVKFVYSNNPES KIMEENFD DKLE+SFGG+
Sbjct: 181 LAILYNPPKLFESFWVMVRPFLESKTSKKVKFVYSNNPESLKIMEENFDADKLESSFGGR 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDI 300
           NP+GFNYE Y+QRMMEDDKKMT FIDS CSSP Y+A+LSKSQ  DSAA  VDSQASD++ 
Sbjct: 241 NPIGFNYEDYSQRMMEDDKKMTHFIDSRCSSPTYKALLSKSQMLDSAAFDVDSQASDDES 300

Query: 301 D-HKISLNLELPNEKLRE 318
           D  +I  NL  P++KL+E
Sbjct: 301 DTDEIPSNLRRPDDKLQE 318

BLAST of Lcy04g007110 vs. NCBI nr
Match: XP_008442047.1 (PREDICTED: random slug protein 5-like isoform X1 [Cucumis melo] >XP_008442048.1 PREDICTED: random slug protein 5-like isoform X1 [Cucumis melo] >KAA0041850.1 random slug protein 5-like isoform X1 [Cucumis melo var. makuwa] >TYK05373.1 random slug protein 5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 535.8 bits (1379), Expect = 2.5e-148
Identity = 269/318 (84.59%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +K R NATEKVLS++EQQA+INEVKK MGPIA KLPVLCSDASILRYLKARNWNTKK
Sbjct: 1   MSARKSRANATEKVLSNQEQQARINEVKKFMGPIAHKLPVLCSDASILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           ANKMLKETLKWRLEYKPEKI WEDIA EAKTGKIYRANYTD+QGRTVLVM PS QNTEST
Sbjct: 61  ANKMLKETLKWRLEYKPEKISWEDIAQEAKTGKIYRANYTDRQGRTVLVMRPSSQNTEST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
           +GQIRYLVYCME+AILSSNS DGYMVWLIDFHGW+TSCLS+KVTR+TA+VLQNHYPERLG
Sbjct: 121 TGQIRYLVYCMEHAILSSNSTDGYMVWLIDFHGWNTSCLSLKVTRDTAHVLQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPKVFESFWVMVRPFLESKT KKVKFVYSNNPES KIM +NFD DKLETSFGGK
Sbjct: 181 LAILYNPPKVFESFWVMVRPFLESKTSKKVKFVYSNNPESLKIMADNFDADKLETSFGGK 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDI 300
           NP+GFNYE Y+QRMMEDDKKMT FIDSGCSSP Y+A+LSKSQ  DSAAS  DSQASD++ 
Sbjct: 241 NPIGFNYEDYSQRMMEDDKKMTRFIDSGCSSPTYKALLSKSQLLDSAASDFDSQASDDES 300

Query: 301 D-HKISLNLELPNEKLRE 318
           D  +I  NL LP++KL+E
Sbjct: 301 DTDEIPSNLGLPDDKLQE 318

BLAST of Lcy04g007110 vs. NCBI nr
Match: XP_023543469.1 (phosphatidylinositol transfer protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 529.3 bits (1362), Expect = 2.4e-146
Identity = 261/318 (82.08%), Postives = 294/318 (92.45%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +K RGN +EKVLSSEEQQAKINEVKKLMGP+ +KLPVLCSDASILRYLKARNWNTKK
Sbjct: 1   MSARKSRGNGSEKVLSSEEQQAKINEVKKLMGPLTEKLPVLCSDASILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           ANKML+ETLKW+LEYKPEKI WEDIALEAKTGKIYRANYTDKQGRTVLVM PS QNTEST
Sbjct: 61  ANKMLRETLKWKLEYKPEKISWEDIALEAKTGKIYRANYTDKQGRTVLVMRPSSQNTEST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
           +GQIRYLVYCMENAIL++NS DGYMVWLIDFHGW+TSCLS+KVTR+TAN+LQNHYPERLG
Sbjct: 121 TGQIRYLVYCMENAILNTNSTDGYMVWLIDFHGWNTSCLSLKVTRDTANILQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPKVFESFWVMVRPFLESKT+KKVKFVYSNNPESQKIME+NF+ ++LETSFGGK
Sbjct: 181 LAILYNPPKVFESFWVMVRPFLESKTFKKVKFVYSNNPESQKIMEDNFNVEELETSFGGK 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDI 300
           NPVGFNYE YA+RM+E DKK T+F+DSG SSP  ++ILSKSQ PDSA S V+SQ SD++ 
Sbjct: 241 NPVGFNYEDYARRMVEGDKKKTEFVDSGFSSPTSKSILSKSQLPDSAVSDVNSQDSDDES 300

Query: 301 DHKIS-LNLELPNEKLRE 318
           D+++   NL LP++KL+E
Sbjct: 301 DNEMQPSNLALPDDKLQE 318

BLAST of Lcy04g007110 vs. NCBI nr
Match: XP_022154468.1 (random slug protein 5-like [Momordica charantia])

HSP 1 Score: 528.1 bits (1359), Expect = 5.3e-146
Identity = 270/316 (85.44%), Postives = 288/316 (91.14%), Query Frame = 0

Query: 1   MSTKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKK 60
           MS +KPRGNATEKVLS EEQQAKI+EVKKLMGPIADKLPVLCS+ SILRYLKARNWNTKK
Sbjct: 1   MSFRKPRGNATEKVLSPEEQQAKIDEVKKLMGPIADKLPVLCSNPSILRYLKARNWNTKK 60

Query: 61  ANKMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTEST 120
           A+KMLKETLKWRLEYKPEKI WEDIA EAKTGKIY+ANYTDKQGRTVLVMTPS QNT+ST
Sbjct: 61  ASKMLKETLKWRLEYKPEKISWEDIADEAKTGKIYKANYTDKQGRTVLVMTPSNQNTDST 120

Query: 121 SGQIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLG 180
            GQIRYLVY MENAILSSNSNDGYMVWLIDFHGW+TS LS+K TRETA VLQNHYPERLG
Sbjct: 121 IGQIRYLVYSMENAILSSNSNDGYMVWLIDFHGWNTSSLSVKTTRETAYVLQNHYPERLG 180

Query: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGK 240
           LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFD +KLETSFGGK
Sbjct: 181 LAILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDEEKLETSFGGK 240

Query: 241 NPVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDS-QASD-E 300
           +P+GFNYE YA+RMMEDDKKMT FID+GCSSP YR ILS SQ PDSAAS  DS +ASD E
Sbjct: 241 SPIGFNYEDYAKRMMEDDKKMTHFIDTGCSSPIYRTILSTSQLPDSAASDADSDEASDHE 300

Query: 301 DIDHKISLNLELPNEK 315
             D +I  NL+ P++K
Sbjct: 301 HSDDEIPSNLKTPDDK 316

BLAST of Lcy04g007110 vs. TAIR 10
Match: AT1G22180.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 338.2 bits (866), Expect = 7.2e-93
Identity = 179/301 (59.47%), Postives = 223/301 (74.09%), Query Frame = 0

Query: 3   TKKPRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKKAN 62
           + K   N  EK L+ EE   KINEV+ L+GP+ +K    CSDA+I RYL ARN + KKA 
Sbjct: 5   SNKSSSNGFEKSLTPEEYLNKINEVRTLLGPLTEKSSEFCSDAAITRYLAARNGHVKKAT 64

Query: 63  KMLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTESTSG 122
           KMLKETLKWR +YKPE+IRWE+IA EA+TGKIYRAN TDK GRTVLVM PSCQNT+S  G
Sbjct: 65  KMLKETLKWRAQYKPEEIRWEEIAREAETGKIYRANCTDKYGRTVLVMRPSCQNTKSYKG 124

Query: 123 QIRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLGLA 182
           QIR LVYCMENAIL+   N   MVWLIDFHG++ S +S+KV+RETA+VLQ HYPERLGLA
Sbjct: 125 QIRILVYCMENAILNLPDNQEQMVWLIDFHGFNMSHISLKVSRETAHVLQEHYPERLGLA 184

Query: 183 ILYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGKN- 242
           I+YNPPK+FESF+ MV+PFLE KT  KVKFVYS++  S K++E+ FD ++LE +FGGKN 
Sbjct: 185 IVYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSDDNLSNKLLEDLFDMEQLEVAFGGKNS 244

Query: 243 PVGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDID 302
             GFN+E YA+RM EDD K   + ++  SS +     S S+  DS    ++ +  DE I+
Sbjct: 245 DAGFNFEKYAERMREDDLKF--YGNTTVSSTSAHLTNSDSEVSDSEMKYLEDK-EDETIE 302

BLAST of Lcy04g007110 vs. TAIR 10
Match: AT4G08690.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 326.6 bits (836), Expect = 2.2e-89
Identity = 162/292 (55.48%), Postives = 213/292 (72.95%), Query Frame = 0

Query: 6   PRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKKANKML 65
           P  N   K + +EE+QAKI EV+KL+GP+ +KL   CSD ++LRYL+ARNW+ KKA KML
Sbjct: 5   PVTNGFVKPVPTEEEQAKIEEVRKLLGPLPEKLSSFCSDDAVLRYLRARNWHVKKATKML 64

Query: 66  KETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTESTSGQIR 125
           KETLKWR++YKPE+I WE++A EA+TGKIYR++  DK GR VL+M PS +N++S  GQIR
Sbjct: 65  KETLKWRVQYKPEEICWEEVAGEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQIR 124

Query: 126 YLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLGLAILY 185
           YLVYCMENA+ +    +  MVW+IDFHG+S + +S++ T+ETA+VLQ HYPERL  A+LY
Sbjct: 125 YLVYCMENAVQNLPPGEEQMVWMIDFHGYSLANVSLRTTKETAHVLQEHYPERLAFAVLY 184

Query: 186 NPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGKNPVGF 245
           NPPK FE FW + RPFLE KT  KVKFVYS++P ++ IMEENFD +K+E +FGG +  GF
Sbjct: 185 NPPKFFEPFWKVARPFLEPKTRNKVKFVYSDDPNTKVIMEENFDMEKMELAFGGNDDSGF 244

Query: 246 NYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASD 298
           N E +++RM EDDKK    ++          I+S S    S  SV D  AS+
Sbjct: 245 NIEKHSERMKEDDKKRLASLE---------GIVSASLDSLSILSVSDGTASN 287

BLAST of Lcy04g007110 vs. TAIR 10
Match: AT4G08690.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 326.6 bits (836), Expect = 2.2e-89
Identity = 162/292 (55.48%), Postives = 213/292 (72.95%), Query Frame = 0

Query: 6   PRGNATEKVLSSEEQQAKINEVKKLMGPIADKLPVLCSDASILRYLKARNWNTKKANKML 65
           P  N   K + +EE+QAKI EV+KL+GP+ +KL   CSD ++LRYL+ARNW+ KKA KML
Sbjct: 5   PVTNGFVKPVPTEEEQAKIEEVRKLLGPLPEKLSSFCSDDAVLRYLRARNWHVKKATKML 64

Query: 66  KETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTESTSGQIR 125
           KETLKWR++YKPE+I WE++A EA+TGKIYR++  DK GR VL+M PS +N++S  GQIR
Sbjct: 65  KETLKWRVQYKPEEICWEEVAGEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQIR 124

Query: 126 YLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLGLAILY 185
           YLVYCMENA+ +    +  MVW+IDFHG+S + +S++ T+ETA+VLQ HYPERL  A+LY
Sbjct: 125 YLVYCMENAVQNLPPGEEQMVWMIDFHGYSLANVSLRTTKETAHVLQEHYPERLAFAVLY 184

Query: 186 NPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGKNPVGF 245
           NPPK FE FW + RPFLE KT  KVKFVYS++P ++ IMEENFD +K+E +FGG +  GF
Sbjct: 185 NPPKFFEPFWKVARPFLEPKTRNKVKFVYSDDPNTKVIMEENFDMEKMELAFGGNDDSGF 244

Query: 246 NYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASD 298
           N E +++RM EDDKK    ++          I+S S    S  SV D  AS+
Sbjct: 245 NIEKHSERMKEDDKKRLASLE---------GIVSASLDSLSILSVSDGTASN 287

BLAST of Lcy04g007110 vs. TAIR 10
Match: AT1G22180.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 284.6 bits (727), Expect = 9.5e-77
Identity = 148/240 (61.67%), Postives = 184/240 (76.67%), Query Frame = 0

Query: 64  MLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTESTSGQ 123
           MLKETLKWR +YKPE+IRWE+IA EA+TGKIYRAN TDK GRTVLVM PSCQNT+S  GQ
Sbjct: 1   MLKETLKWRAQYKPEEIRWEEIAREAETGKIYRANCTDKYGRTVLVMRPSCQNTKSYKGQ 60

Query: 124 IRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLGLAI 183
           IR LVYCMENAIL+   N   MVWLIDFHG++ S +S+KV+RETA+VLQ HYPERLGLAI
Sbjct: 61  IRILVYCMENAILNLPDNQEQMVWLIDFHGFNMSHISLKVSRETAHVLQEHYPERLGLAI 120

Query: 184 LYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGKN-P 243
           +YNPPK+FESF+ MV+PFLE KT  KVKFVYS++  S K++E+ FD ++LE +FGGKN  
Sbjct: 121 VYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSDDNLSNKLLEDLFDMEQLEVAFGGKNSD 180

Query: 244 VGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDIDH 303
            GFN+E YA+RM EDD K   + ++  SS +     S S+  DS    ++ +  DE I++
Sbjct: 181 AGFNFEKYAERMREDDLKF--YGNTTVSSTSAHLTNSDSEVSDSEMKYLEDK-EDETIEN 237

BLAST of Lcy04g007110 vs. TAIR 10
Match: AT1G22180.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 284.6 bits (727), Expect = 9.5e-77
Identity = 148/240 (61.67%), Postives = 184/240 (76.67%), Query Frame = 0

Query: 64  MLKETLKWRLEYKPEKIRWEDIALEAKTGKIYRANYTDKQGRTVLVMTPSCQNTESTSGQ 123
           MLKETLKWR +YKPE+IRWE+IA EA+TGKIYRAN TDK GRTVLVM PSCQNT+S  GQ
Sbjct: 1   MLKETLKWRAQYKPEEIRWEEIAREAETGKIYRANCTDKYGRTVLVMRPSCQNTKSYKGQ 60

Query: 124 IRYLVYCMENAILSSNSNDGYMVWLIDFHGWSTSCLSMKVTRETANVLQNHYPERLGLAI 183
           IR LVYCMENAIL+   N   MVWLIDFHG++ S +S+KV+RETA+VLQ HYPERLGLAI
Sbjct: 61  IRILVYCMENAILNLPDNQEQMVWLIDFHGFNMSHISLKVSRETAHVLQEHYPERLGLAI 120

Query: 184 LYNPPKVFESFWVMVRPFLESKTYKKVKFVYSNNPESQKIMEENFDRDKLETSFGGKN-P 243
           +YNPPK+FESF+ MV+PFLE KT  KVKFVYS++  S K++E+ FD ++LE +FGGKN  
Sbjct: 121 VYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSDDNLSNKLLEDLFDMEQLEVAFGGKNSD 180

Query: 244 VGFNYEAYAQRMMEDDKKMTDFIDSGCSSPNYRAILSKSQPPDSAASVVDSQASDEDIDH 303
            GFN+E YA+RM EDD K   + ++  SS +     S S+  DS    ++ +  DE I++
Sbjct: 181 AGFNFEKYAERMREDDLKF--YGNTTVSSTSAHLTNSDSEVSDSEMKYLEDK-EDETIEN 237

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q55CU81.3e-2528.40Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=... [more]
Q9UU994.5e-2334.12CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (stra... [more]
P538445.7e-1829.96Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ... [more]
P538607.4e-1825.46Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ... [more]
P248592.5e-1328.16SEC14 cytosolic factor OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DS... [more]
Match NameE-valueIdentityDescription
A0A0A0L1I27.2e-14985.22CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G594430 P... [more]
A0A1S3B5J61.2e-14884.59random slug protein 5-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486028 PE... [more]
A0A5D3C0751.2e-14884.59Random slug protein 5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1DKE02.6e-14685.44random slug protein 5-like OS=Momordica charantia OX=3673 GN=LOC111021741 PE=4 S... [more]
A0A6J1GFS43.3e-14681.76phosphatidylinositol transfer protein 3-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_038881392.11.2e-15086.12phosphatidylinositol transfer protein 3-like [Benincasa hispida][more]
XP_011653957.11.5e-14885.22phosphatidylinositol transfer protein 3 isoform X1 [Cucumis sativus] >XP_0317395... [more]
XP_008442047.12.5e-14884.59PREDICTED: random slug protein 5-like isoform X1 [Cucumis melo] >XP_008442048.1 ... [more]
XP_023543469.12.4e-14682.08phosphatidylinositol transfer protein 3-like [Cucurbita pepo subsp. pepo][more]
XP_022154468.15.3e-14685.44random slug protein 5-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G22180.27.2e-9359.47Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G08690.22.2e-8955.48Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G08690.12.2e-8955.48Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G22180.19.5e-7761.67Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G22180.39.5e-7761.67Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011074CRAL/TRIO, N-terminal domainSMARTSM01100CRAL_TRIO_N_2coord: 43..68
e-value: 3.3E-5
score: 33.3
IPR011074CRAL/TRIO, N-terminal domainPFAMPF03765CRAL_TRIO_Ncoord: 43..67
e-value: 1.8E-6
score: 28.0
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 88..242
e-value: 1.3E-37
score: 140.9
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 89..240
e-value: 1.1E-31
score: 109.7
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 82..245
score: 15.875284
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 90..240
e-value: 2.86695E-34
score: 120.953
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 2..270
e-value: 6.8E-74
score: 250.8
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 82..260
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 275..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 275..297
NoneNo IPR availablePANTHERPTHR45824:SF18OS01G0264700 PROTEINcoord: 1..298
NoneNo IPR availablePANTHERPTHR45824GH16843Pcoord: 1..298
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 17..84

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g007110.1Lcy04g007110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0120009 intermembrane lipid transfer
biological_process GO:0015914 phospholipid transport
cellular_component GO:0016020 membrane
molecular_function GO:0008526 phosphatidylinositol transfer activity