PI0001227 (gene) Melon (PI 482460) v1

BLAST of PI0001227 vs. ExPASy Swiss-Prot
Match: P40340 (Tat-binding homolog 7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YTA7 PE=1 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 1.3e-26
Identity = 130/518 (25.10%), Postives = 211/518 (40.73%), Query Frame = 0

Query: 202 DSNQEDDQQEMQPIFIGGTATHSNSNNNKKEELVMG-----------GWESVAGLQNVIQ 261
           DS+  DD  E+ P+ +       N+   KK++  +             ++ + GL N I 
Sbjct: 366 DSDSSDD--EILPLGVTPKTKKENTQKKKKKKPEIADLDPLGVDMNVNFDDIGGLDNYID 425

Query: 262 CMKEAVVLPLLYPELFRGLAITH----------------VVRALVASCARANKIVAYFSR 321
            +KE V LPLLYPEL++   IT                 + RAL ASC+   + + +F R
Sbjct: 426 QLKEMVALPLLYPELYQNFNITPPRGVLFHGPPGTGKTLMARALAASCSSDERKITFFMR 485

Query: 322 KGADCLGKNVNS------------------------------------------------ 381
           KGAD L K V                                                  
Sbjct: 486 KGADILSKWVGEAERQLRLLFEEAKKHQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLL 545

Query: 382 ---------------------NVIDPALRRPGRFDREIYFPLPSFEDRISILSLYTQKWP 441
                                + +DPALRRPGRFDRE YFPLP  + R  IL + T+KW 
Sbjct: 546 ALMDGMDNRGQVIVIGATNRPDAVDPALRRPGRFDREFYFPLPDVKARFKILQIQTRKWS 605

Query: 442 KTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINALKRTFPLKQVLSATCDPHPTQLPV 501
             L++N   +  +A  T G+ GADL++LCT+ A+ +++R+FP     +      P+++ V
Sbjct: 606 SPLSTN--FIDKLAFLTKGYGGADLRSLCTEAALISIQRSFPQIYRSNDKLLVDPSKIKV 665

Query: 502 VVEDKDWLEALSTCPPPCSRREALAALNETASSPLPIHLIPCLIQPLSTLLVSLYFEESI 561
            V   D++ AL    P  +R         T SSP P   +P LI+PL             
Sbjct: 666 KV--SDFMLALKKIVPSSAR--------STGSSPQP---LPELIKPL------------- 725

Query: 562 CLPKTLLKALERKSTKWWFHLNNFNQDSEIRIKRKWQGSGANILEHSSSVNSGKFISEED 614
                L   L     K  + LN         IK        ++L+  + ++  ++  EE+
Sbjct: 726 -----LADQLNNLKNKLDYMLN---------IKDTTFQRNTSLLQ--NFIDYEEYSGEEE 785

BLAST of PI0001227 vs. ExPASy Swiss-Prot
Match: O14114 (Uncharacterized AAA domain-containing protein C31G5.19 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC31G5.19 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 8.1e-26
Identity = 115/438 (26.26%), Postives = 174/438 (39.73%), Query Frame = 0

Query: 239 WESVAGLQNVIQCMKEAVVLPLLYPELFR----------------GLAITHVVRALVASC 298
           +ESV GL N I  +KE V+LPLLYPE+F+                G   T + RAL A+C
Sbjct: 265 FESVGGLDNYINQLKEMVMLPLLYPEIFQRFNMQPPRGVLFHGPPGTGKTLMARALAAAC 324

Query: 299 ARANKIVAYFSRKGADCLGKNVNS------------------------------------ 358
           +  NK V+++ RKGADCL K V                                      
Sbjct: 325 SSENKKVSFYMRKGADCLSKWVGEAERQLRLLFEEAKSTQPSIIFFDEIDGLAPVRSSKQ 384

Query: 359 ---------------------------------NVIDPALRRPGRFDREIYFPLPSFEDR 418
                                            + +DPALRRPGRFDRE YFPLP  + R
Sbjct: 385 EQIHASIVSTLLALMDGMESRGQVIIIGATNRPDAVDPALRRPGRFDREFYFPLPDRDAR 444

Query: 419 ISILSLYTQKWPKTLNSNQPLLHW----IAKKTSGFAGADLQALCTQTAINALKRTFPLK 478
             I+ ++T+ W      + P+  W    +A+K+ G+ GADL+ALCT+ A+N++KRT+P  
Sbjct: 445 KKIIEIHTRNW------DPPVPEWLCSMLAEKSKGYGGADLRALCTEAALNSIKRTYP-- 504

Query: 479 QVLSATCDPHPTQLPVVVEDKDWLEALSTCPPPCSRREALAALNETASSPLPIHLIPCLI 538
           Q+  +T         + V+ KD++ ++    P   R         + S PL   L P   
Sbjct: 505 QLYRSTKRLQIDPKTIKVKVKDFVMSMKRMIPSSERSSI------SPSKPLSPELKPL-- 564

Query: 539 QPLSTLLVSLYFEESICLPKTLLKALERKSTKWWFHLNNFNQDSEIRIKRKWQGSGANIL 587
                                               LN   QD E  +++        ++
Sbjct: 565 ------------------------------------LNEAFQDIEKTLQK--------LM 624

BLAST of PI0001227 vs. ExPASy Swiss-Prot
Match: Q9C0W2 (Uncharacterized AAA domain-containing protein P22H7.05c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pi026 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 2.2e-23
Identity = 76/234 (32.48%), Postives = 109/234 (46.58%), Query Frame = 0

Query: 239 WESVAGLQNVIQCMKEAVVLPLLYPELFRGLAITH----------------VVRALVASC 298
           + S+ GL+++I  +KE V+LPLLYPE+F  L IT                 + R L A+C
Sbjct: 371 FNSIGGLEDIILQLKEMVMLPLLYPEVFLHLHITPPRGVLFHGPPGTGKTLMARVLAANC 430

Query: 299 ARANKIVAYFSRKGADCLGKNVNS------------------------------------ 358
           +  N+ +++F RKG+DCL K V                                      
Sbjct: 431 STKNQKISFFLRKGSDCLSKWVGEAERQLRLLFEEARRVQPSIIFFDEIDGLAPIRSSKQ 490

Query: 359 ---------------------------------NVIDPALRRPGRFDREIYFPLPSFEDR 388
                                            N +DPALRRPGRFDRE YFPLP+ + R
Sbjct: 491 EQTHSSIVSTLLALMDGLDTRGQVVVIGATNRPNDLDPALRRPGRFDREFYFPLPNKQAR 550

BLAST of PI0001227 vs. ExPASy Swiss-Prot
Match: Q9ULI0 (ATPase family AAA domain-containing protein 2B OS=Homo sapiens OX=9606 GN=ATAD2B PE=1 SV=3)

HSP 1 Score: 105.1 bits (261), Expect = 4.6e-21
Identity = 130/537 (24.21%), Postives = 218/537 (40.60%), Query Frame = 0

Query: 168 IFQLETHLNSPEKIEFKMGKQYSKEDHKCIDKDDDSNQEDDQQEMQPIFIGGTATHSNSN 227
           I   +T  +  E+ E +  K  ++  ++C+  +  +         + + +G +    +  
Sbjct: 332 IHSSDTTSSDEERFERRKSKSMARARNRCLPMNFRAEDLASGILRERVKVGASLADVDPM 391

Query: 228 NNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELFR----------------GLAI 287
           N  K       ++S+ GL + I  +KE VV PLLYPE+F                 G   
Sbjct: 392 NIDKSV----RFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGK 451

Query: 288 THVVRALVASCARANKIVAYFSRKGADCLGK----------------------------- 347
           T V RAL   C++ +K VA+F RKGADCL K                             
Sbjct: 452 TLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEI 511

Query: 348 ------------NVNSNV----------------------------IDPALRRPGRFDRE 407
                        ++S++                            IDPALRRPGRFDRE
Sbjct: 512 DGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDRE 571

Query: 408 IYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINAL 467
             F LP  + R  IL ++T+ W   L  +   L  +A+K  G+ GAD++ALCT+ A+ AL
Sbjct: 572 FLFNLPDQKARKHILQIHTRDWNPKL--SDAFLGELAEKCVGYCGADIKALCTEAALIAL 631

Query: 468 KRTFPLKQVLSATCDPHPTQLPV---VVEDKDWLEALSTCPPPCSRREALAALNETASSP 527
           +R +P  Q+ +++   H  QL V   V+  +D+  A+     P S+R  +++        
Sbjct: 632 RRRYP--QIYASS---HKLQLDVSSIVLSAQDFYHAMQNI-VPASQRAVMSS-------- 691

Query: 528 LPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLKALERKSTKWWFHLNNFNQDSEIRIKR 587
              H +  +I+PL         E S      +L  L++         ++  +D E  I  
Sbjct: 692 --GHALSPIIRPL--------LERSF---NNILAVLQKVFPHAEISQSDKKEDIETLILE 751

Query: 588 KWQGSGA-NILEHSSSVNSGKFISEEDTSTTDHENSNQTSFDLRNSFGFRIMIAGNPRSG 614
             +   A +I E +    S K  S        + +   + +    S+  R++++G   SG
Sbjct: 752 DSEDENALSIFETNCHSGSPKKQSSSAAIHKPYLHFTMSPYHQPTSYRPRLLLSGERGSG 811

BLAST of PI0001227 vs. ExPASy Swiss-Prot
Match: A8X0L9 (Tat-binding homolog 7 OS=Caenorhabditis briggsae OX=6238 GN=lex-1 PE=3 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 2.3e-20
Identity = 73/235 (31.06%), Postives = 95/235 (40.43%), Query Frame = 0

Query: 238 GWESVAGLQNVIQCMKEAVVLPLLYPELFR----------------GLAITHVVRALVAS 297
           G++ V GL + IQ +KE V+ P+LYPE+F                 G   T V RAL   
Sbjct: 403 GFDQVGGLSHHIQSLKEVVLFPMLYPEVFAKFKINPPKGVVFYGPPGTGKTLVARALANE 462

Query: 298 CARANKIVAYFSRKGADCLGKNVNS----------------------------------- 357
           C R    VA+F RKGADCL K V                                     
Sbjct: 463 CRRGANKVAFFMRKGADCLSKWVGESERQLRLLFDQAYAMRPSIIFFDEIDGLAPVRSSK 522

Query: 358 ----------------------------------NVIDPALRRPGRFDREIYFPLPSFED 388
                                             + +DPALRRPGRFDRE+ F LP    
Sbjct: 523 QDQIHASIVSTLLALMDGLDGRGEVVVIGATNRLDSLDPALRRPGRFDRELRFSLPDLNA 582

BLAST of PI0001227 vs. ExPASy TrEMBL
Match: A0A6J1DS84 (uncharacterized protein LOC111023817 OS=Momordica charantia OX=3673 GN=LOC111023817 PE=4 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 2.0e-253
Identity = 526/1054 (49.91%), Postives = 630/1054 (59.77%), Query Frame = 0

Query: 2    MKWNHKKEGVSDDSE--------GEKLSVKRRKGMENENKKLDDE---WRHCELCGGGSD 61
            MK  HK    SDD E        G K  +K ++  + +N++++ E   WRHC LCGG + 
Sbjct: 321  MKRKHK----SDDREESRGALKLGSKRKMKTKRSEKEQNEEVEAEESSWRHCVLCGGKAK 380

Query: 62   GKPPILMEHN-----NNDDEFGDEDGWLGHLVGPIIDHNVIPRKWLHYQCVIWSPVVHFT 121
                    HN     N++D FGDEDGWLG L+GP  D N IPR W+HYQC+IWSP V+  
Sbjct: 381  LAANSHHNHNHDHDDNDNDGFGDEDGWLGRLLGPFTDSNSIPRLWVHYQCIIWSPEVYVD 440

Query: 122  ESVSLKNVQAALKRGKSLKCTHCKRRGATLGCRLEQCQKTYHL--VRS---KFDHKQFLM 181
            E+  L++V+AAL RGKSLKC+HCKRRGATLGCR+++CQKTYHL   RS   KF HKQFL+
Sbjct: 441  ETGRLRDVKAALNRGKSLKCSHCKRRGATLGCRIKECQKTYHLACARSNGCKFYHKQFLI 500

Query: 182  ACTDHGHIFQLETHLNSPEKIEFKMGKQYSKED----HKCIDKDDDSNQED----DQQEM 241
            AC DH HIF      ++ +KI+ +  K+  +      H+    DD    E+    + ++ 
Sbjct: 501  ACPDHLHIFHHPRDDSNSDKIKLRARKEKLETSNHARHEDTQTDDGGGNEEFSKQNSKKS 560

Query: 242  QPIFIGGTATHSNSNNNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELFRGLAI- 301
               F   T T+      K+E  +  GWESVAGLQ+VI+CMKE VVLPLLYPE F GL I 
Sbjct: 561  NQEFRKITPTYIGGEGEKEESHMSAGWESVAGLQHVIECMKEVVVLPLLYPEFFEGLGIV 620

Query: 302  ---------------THVVRALVASCARANKIVAYFSRKGADCLGKNVNS---------- 361
                           THVVRALV SCAR NK +AYFSRKGADCLGK V            
Sbjct: 621  PPRGVLLHGYPGTGKTHVVRALVGSCARGNKXIAYFSRKGADCLGKYVGDSEQLLRRLFQ 680

Query: 362  -----------------------------------------------------------N 421
                                                                       +
Sbjct: 681  VAQDCQPSIIFFDEIDGLAPCRTRKQDQTHTSVVSTLLALLDGLKSRGSXVVIGATNRPD 740

Query: 422  VIDPALRRPGRFDREIYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAG 481
             IDPALRRPGRFDREIYFPLPS EDRISIL LYT KWPKTL SN PLL WIA+KT GFAG
Sbjct: 741  AIDPALRRPGRFDREIYFPLPSLEDRISILKLYTHKWPKTLESNGPLLQWIARKTLGFAG 800

Query: 482  ADLQALCTQTAINALKRTFPLKQVLSATCDPH---PTQL-PVVVEDKDWLEALSTCPPPC 541
            ADLQALCTQTAI ALKRTFPLKQVL A+ D     PT L PV+VE++DWLEALS+CPPPC
Sbjct: 801  ADLQALCTQTAIIALKRTFPLKQVLHASKDTKYGPPTHLPPVIVEERDWLEALSSCPPPC 860

Query: 542  SRREALAALNETASSPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTL-----------L 601
            SRREA  A N+  SSPLP HLIPCLI+PLS LLVSLY E+ ICLP+TL           L
Sbjct: 861  SRREATMAANDVVSSPLPSHLIPCLIRPLSILLVSLYLEKCICLPQTLSKAAAIIENVML 920

Query: 602  KALERK---STKWWFHLNNFNQDSEI--RIKRKWQGSGANILEHSSSVNSGKFISEEDTS 661
             ALERK   S KWW H+++F Q+ +I   I+RKWQ    NILEHS   NS + I E    
Sbjct: 921  SALERKTXNSNKWWSHVDDFIQEDDIADEIERKWQDCRTNILEHSCLANSYESIPE---- 980

Query: 662  TTDHENSNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQG 721
                +N+  TSFDL NS GFRIMIAGNP  GQRHLASCILNCFV HV IRKVDMAT SQG
Sbjct: 981  ----DNAKYTSFDLGNSQGFRIMIAGNPSLGQRHLASCILNCFVGHVEIRKVDMATTSQG 1040

Query: 722  YATLGQGIAYAFDNTFLRESCLIFMPR--------------------------------- 781
            Y  L QGI++   N   R  CLIFMPR                                 
Sbjct: 1041 YNNLEQGISHTLANCLSRGPCLIFMPRIDLWALETHEDQTLEHDNTHPTDEQCIRKAIVH 1100

Query: 782  -------------------------ATSEIPFLQLPREIRHFFQNDVSNCKMLGARAHKI 841
                                     ATSE+PFL LP+EIR FF ND+++CK L A  HKI
Sbjct: 1101 RASSAWTLFIYQVESLSLCTPXIFLATSEVPFLLLPQEIRLFFLNDLTDCKRLVASKHKI 1160

Query: 842  PRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKLLVLLIHQNNHVRDTNACNSYNPPSDFI 863
            PRFSIQID+  T +H+L+I++SAA LS D++KLL LLIHQ  HV      +     S++ 
Sbjct: 1161 PRFSIQIDE--TFNHDLVIDRSAATLSKDMVKLLALLIHQKYHVNXNEXHSEXTRSSNYQ 1220

BLAST of PI0001227 vs. ExPASy TrEMBL
Match: A0A5A7UUP2 (Tat-binding-7-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold803G00320 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 4.7e-202
Identity = 505/1443 (35.00%), Postives = 679/1443 (47.05%), Query Frame = 0

Query: 38   EWRHCELCGGGSDGKPPILMEHNNND--------------------DEFGDEDGWLGHLV 97
            E R C LCGGG DGKPP  M  ++ +                    D FGDE GWLG L+
Sbjct: 411  EGRRCGLCGGGIDGKPPKKMAQDSGESGNEACSGSSASEEPNYDKWDGFGDEPGWLGRLL 470

Query: 98   GPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLKCTHCKRRGATLGC 157
            GPI D   I   W+H  C +WSP V+F     LKNV+AAL RG++LKCT C R GAT+GC
Sbjct: 471  GPINDRYGIAGIWVHQHCAVWSPEVYFAGLGCLKNVRAALCRGRALKCTRCGRPGATIGC 530

Query: 158  RLEQCQKTYHLVRSK-----FDHKQFLMACTDHGHIFQLETHLN---------SPEKIEF 217
            R+++C KTYHL  ++     FDH++FL+ACTDH HIFQ   H N           +K++ 
Sbjct: 531  RVDRCPKTYHLPCARANGCIFDHRKFLIACTDHRHIFQ--PHGNQYLARIKRLKAKKMKL 590

Query: 218  KMGKQYSKEDHKCIDKDDD--SNQEDDQQ--------------EMQPIFIGGTATHSNSN 277
            ++ KQ +    + I+ ++    N  +D++               + P++IGG        
Sbjct: 591  EIKKQSNDAWRRDIEAEEKWLENCGEDEEFLKRESKRLHRDLVRIAPVYIGG-------- 650

Query: 278  NNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELF--------RGLAI-------- 337
            +N + E +  GWESVAGLQ VIQCMKE V LPLLYPELF        RG+ +        
Sbjct: 651  SNSEGENLFHGWESVAGLQGVIQCMKEVVFLPLLYPELFDRFGITPPRGVLLHGYPGTGK 710

Query: 338  THVVRALVASCARANKIVAYFSRKGADCLGKNVNS------------------------- 397
            THVVRAL+ SCAR +K +AYF+RKGADCLGK V                           
Sbjct: 711  THVVRALIGSCARGDKRIAYFARKGADCLGKYVGDAERQLRLLFQVAERCQPSIIFFDEI 770

Query: 398  --------------------------------------------NVIDPALRRPGRFDRE 457
                                                          +DPALRRPGRFDRE
Sbjct: 771  DGLAPCRTRQQDQTHNSVVSTLLALLDGLKSRGSVVVIGATNRPEAVDPALRRPGRFDRE 830

Query: 458  IYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINAL 517
            IYFPLPS EDR +ILSL+TQKWPK ++   PLL WIA++T+GFAGADLQALCTQ A++AL
Sbjct: 831  IYFPLPSVEDRAAILSLHTQKWPKPIDG--PLLQWIARRTAGFAGADLQALCTQAAMSAL 890

Query: 518  KRTFPLKQVLSATCDP-----HPTQLPVVVEDKDWLEALSTCPPPCSRREALAALNETAS 577
            KR FPLK+VLSA+ +       P    ++VE++DWLEAL   PPPCSRREA  A N+ AS
Sbjct: 891  KRNFPLKEVLSASGEQVLRVNRPPLPSILVEERDWLEALLYSPPPCSRREAGMAANDVAS 950

Query: 578  SPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLK-----------ALERK---STKWW 637
            SPLP HLIPCL+QPLSTLLVSLY +E I LP  LLK           AL+ K   ++ WW
Sbjct: 951  SPLPFHLIPCLLQPLSTLLVSLYLDERITLPTNLLKAATSIKSVIVSALDGKNIVTSCWW 1010

Query: 638  FHLNNFNQDSEI--RIKRKWQGSGANILEHSSSVNSGKFISEEDTSTTDHEN-------- 697
             H+++F QD++I   I+ K QGSG  ++E S+  +SG    +    ++  EN        
Sbjct: 1011 SHVHDFVQDADIANEIEIKLQGSGV-LVEDSTFGSSGVLNIDTGNESSKFENLGHCGGRP 1070

Query: 698  ---SNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-GYA 757
                  TSF L N  GFRI+IAGN RSG RHLASC+++C+++HV +RKVD+ATISQ G+ 
Sbjct: 1071 ATMVEHTSFTLGNKSGFRILIAGNTRSGPRHLASCLIHCYIQHVEVRKVDIATISQEGHG 1130

Query: 758  TLGQGIAYAFDNTFLRESCLIFMPR----------------------------------- 817
             L  GI+    N     +CL+FMPR                                   
Sbjct: 1131 DLVHGISQILLNCSSMGACLVFMPRIDLWAIETQSQTSEECGFYLNEDEHVEDGIIVKDE 1190

Query: 818  ----------------------------------------------------ATSEIPFL 877
                                                                ATSE+PFL
Sbjct: 1191 GQLGERDDCRYPDQSKSSERTGLQDECLSSASYAWGSFVEQVESLSTPLMILATSEVPFL 1250

Query: 878  QLPREIRHFFQNDVSNCKMLGARAHKIPRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKL 937
             LP+EIR FF+ND+S C+   +  H +PRFS+QI  DG  DH+++INQSAA+LS D++KL
Sbjct: 1251 LLPQEIREFFRNDLSMCRPTTSE-HSVPRFSVQI--DGVFDHDMVINQSAAELSRDIVKL 1310

Query: 938  LVLLIHQNNHVRDTNACNSYNPP----------SDFIEDQAPKHK--------------- 997
            LV LIHQ +H + T+ C  Y  P              ++ A +H                
Sbjct: 1311 LVHLIHQKSHTK-TSTCTKYQIPVIQGENNAENQQIDKETASEHNGEMKSPDVSSLRIAP 1370

Query: 998  ---------HTNLNSEISNVGYKILQNPQYSNLCWVTSNFKEGPSANLSKSQWQDWKFNP 999
                      +NL S IS  G++IL+ P ++ LCWVTS  KEGP A++S   W+ W FN 
Sbjct: 1371 LPGSRTMKVKSNLISVISTFGHQILRYPHFAELCWVTSKLKEGPYADVS-GPWKGWPFNS 1430

BLAST of PI0001227 vs. ExPASy TrEMBL
Match: A0A1S3C2T2 (uncharacterized protein LOC103496212 OS=Cucumis melo OX=3656 GN=LOC103496212 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 4.7e-202
Identity = 505/1443 (35.00%), Postives = 679/1443 (47.05%), Query Frame = 0

Query: 38   EWRHCELCGGGSDGKPPILMEHNNND--------------------DEFGDEDGWLGHLV 97
            E R C LCGGG DGKPP  M  ++ +                    D FGDE GWLG L+
Sbjct: 476  EGRRCGLCGGGIDGKPPKKMAQDSGESGNEACSGSSASEEPNYDKWDGFGDEPGWLGRLL 535

Query: 98   GPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLKCTHCKRRGATLGC 157
            GPI D   I   W+H  C +WSP V+F     LKNV+AAL RG++LKCT C R GAT+GC
Sbjct: 536  GPINDRYGIAGIWVHQHCAVWSPEVYFAGLGCLKNVRAALCRGRALKCTRCGRPGATIGC 595

Query: 158  RLEQCQKTYHLVRSK-----FDHKQFLMACTDHGHIFQLETHLN---------SPEKIEF 217
            R+++C KTYHL  ++     FDH++FL+ACTDH HIFQ   H N           +K++ 
Sbjct: 596  RVDRCPKTYHLPCARANGCIFDHRKFLIACTDHRHIFQ--PHGNQYLARIKRLKAKKMKL 655

Query: 218  KMGKQYSKEDHKCIDKDDD--SNQEDDQQ--------------EMQPIFIGGTATHSNSN 277
            ++ KQ +    + I+ ++    N  +D++               + P++IGG        
Sbjct: 656  EIKKQSNDAWRRDIEAEEKWLENCGEDEEFLKRESKRLHRDLVRIAPVYIGG-------- 715

Query: 278  NNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELF--------RGLAI-------- 337
            +N + E +  GWESVAGLQ VIQCMKE V LPLLYPELF        RG+ +        
Sbjct: 716  SNSEGENLFHGWESVAGLQGVIQCMKEVVFLPLLYPELFDRFGITPPRGVLLHGYPGTGK 775

Query: 338  THVVRALVASCARANKIVAYFSRKGADCLGKNVNS------------------------- 397
            THVVRAL+ SCAR +K +AYF+RKGADCLGK V                           
Sbjct: 776  THVVRALIGSCARGDKRIAYFARKGADCLGKYVGDAERQLRLLFQVAERCQPSIIFFDEI 835

Query: 398  --------------------------------------------NVIDPALRRPGRFDRE 457
                                                          +DPALRRPGRFDRE
Sbjct: 836  DGLAPCRTRQQDQTHNSVVSTLLALLDGLKSRGSVVVIGATNRPEAVDPALRRPGRFDRE 895

Query: 458  IYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINAL 517
            IYFPLPS EDR +ILSL+TQKWPK ++   PLL WIA++T+GFAGADLQALCTQ A++AL
Sbjct: 896  IYFPLPSVEDRAAILSLHTQKWPKPIDG--PLLQWIARRTAGFAGADLQALCTQAAMSAL 955

Query: 518  KRTFPLKQVLSATCDP-----HPTQLPVVVEDKDWLEALSTCPPPCSRREALAALNETAS 577
            KR FPLK+VLSA+ +       P    ++VE++DWLEAL   PPPCSRREA  A N+ AS
Sbjct: 956  KRNFPLKEVLSASGEQVLRVNRPPLPSILVEERDWLEALLYSPPPCSRREAGMAANDVAS 1015

Query: 578  SPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLK-----------ALERK---STKWW 637
            SPLP HLIPCL+QPLSTLLVSLY +E I LP  LLK           AL+ K   ++ WW
Sbjct: 1016 SPLPFHLIPCLLQPLSTLLVSLYLDERITLPTNLLKAATSIKSVIVSALDGKNIVTSCWW 1075

Query: 638  FHLNNFNQDSEI--RIKRKWQGSGANILEHSSSVNSGKFISEEDTSTTDHEN-------- 697
             H+++F QD++I   I+ K QGSG  ++E S+  +SG    +    ++  EN        
Sbjct: 1076 SHVHDFVQDADIANEIEIKLQGSGV-LVEDSTFGSSGVLNIDTGNESSKFENLGHCGGRP 1135

Query: 698  ---SNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-GYA 757
                  TSF L N  GFRI+IAGN RSG RHLASC+++C+++HV +RKVD+ATISQ G+ 
Sbjct: 1136 ATMVEHTSFTLGNKSGFRILIAGNTRSGPRHLASCLIHCYIQHVEVRKVDIATISQEGHG 1195

Query: 758  TLGQGIAYAFDNTFLRESCLIFMPR----------------------------------- 817
             L  GI+    N     +CL+FMPR                                   
Sbjct: 1196 DLVHGISQILLNCSSMGACLVFMPRIDLWAIETQSQTSEECGFYLNEDEHVEDGIIVKDE 1255

Query: 818  ----------------------------------------------------ATSEIPFL 877
                                                                ATSE+PFL
Sbjct: 1256 GQLGERDDCRYPDQSKSSERTGLQDECLSSASYAWGSFVEQVESLSTPLMILATSEVPFL 1315

Query: 878  QLPREIRHFFQNDVSNCKMLGARAHKIPRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKL 937
             LP+EIR FF+ND+S C+   +  H +PRFS+QI  DG  DH+++INQSAA+LS D++KL
Sbjct: 1316 LLPQEIREFFRNDLSMCRPTTSE-HSVPRFSVQI--DGVFDHDMVINQSAAELSRDIVKL 1375

Query: 938  LVLLIHQNNHVRDTNACNSYNPP----------SDFIEDQAPKHK--------------- 997
            LV LIHQ +H + T+ C  Y  P              ++ A +H                
Sbjct: 1376 LVHLIHQKSHTK-TSTCTKYQIPVIQGENNAENQQIDKETASEHNGEMKSPDVSSLRIAP 1435

Query: 998  ---------HTNLNSEISNVGYKILQNPQYSNLCWVTSNFKEGPSANLSKSQWQDWKFNP 999
                      +NL S IS  G++IL+ P ++ LCWVTS  KEGP A++S   W+ W FN 
Sbjct: 1436 LPGSRTMKVKSNLISVISTFGHQILRYPHFAELCWVTSKLKEGPYADVS-GPWKGWPFNS 1495

BLAST of PI0001227 vs. ExPASy TrEMBL
Match: A0A0A0L9H9 (PHD-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G236020 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 6.2e-202
Identity = 516/1480 (34.86%), Postives = 690/1480 (46.62%), Query Frame = 0

Query: 1    MMKWNHKKEGVSDDSEGEKLSVKRRKGMENENKKLDDEWRHCELCGGGSDGKPP------ 60
            M K+  K     DD   +K +   R  +     K   E R C LCGGG DGKPP      
Sbjct: 430  MGKFTEKSREHGDDLNLKKFTDSSRGMLGKARIK---EGRRCGLCGGGIDGKPPKKTAQD 489

Query: 61   ------------ILMEHNNND--DEFGDEDGWLGHLVGPIIDHNVIPRKWLHYQCVIWSP 120
                           E  N D  D FGDE GWLG L+GPI D   I   W+H  C +WSP
Sbjct: 490  SGESGNEACSGSSASEEPNYDKWDGFGDEPGWLGRLLGPINDRYGIAGIWVHQHCAVWSP 549

Query: 121  VVHFTESVSLKNVQAALKRGKSLKCTHCKRRGATLGCRLEQCQKTYHLVRSK-----FDH 180
             V+F     LKNV+AAL RG++LKCT C R GAT+GCR+++C +TYHL  ++     FDH
Sbjct: 550  EVYFAGLGCLKNVRAALCRGRALKCTRCGRPGATIGCRVDRCPRTYHLPCARANGCIFDH 609

Query: 181  KQFLMACTDHGHIFQLETHLN---------SPEKIEFKMGKQYSKEDHKCIDKDDD--SN 240
            ++FL+ACTDH HIFQ   H N           +K++ ++ KQ +    + I+ ++    N
Sbjct: 610  RKFLIACTDHRHIFQ--PHGNQYLARIKRLKAKKMKLEIKKQSNDAWRRDIEAEEKWLEN 669

Query: 241  QEDDQQ--------------EMQPIFIGGTATHSNSNNNKKEELVMGGWESVAGLQNVIQ 300
              +D++               + P++IGG        +N + E +  GWESVAGLQ VIQ
Sbjct: 670  CGEDEEFLKRESKRLHRDLVRIAPVYIGG--------SNSEGENLFHGWESVAGLQGVIQ 729

Query: 301  CMKEAVVLPLLYPELF--------RGLAI--------THVVRALVASCARANKIVAYFSR 360
            CMKE V LPLLYPELF        RG+ +        THVVRAL+ SCAR +K +AYF+R
Sbjct: 730  CMKEVVFLPLLYPELFDRFGITPPRGVLLHGYPGTGKTHVVRALIGSCARGDKRIAYFAR 789

Query: 361  KGADCLGKNVNS------------------------------------------------ 420
            KGADCLGK V                                                  
Sbjct: 790  KGADCLGKYVGDAERQLRLLFQVAEKCQPSIIFFDEIDGLAPCRTRQQDQTHNSVVSTLL 849

Query: 421  ---------------------NVIDPALRRPGRFDREIYFPLPSFEDRISILSLYTQKWP 480
                                   +DPALRRPGRFDREIYFPLPS EDR +ILSL+TQKWP
Sbjct: 850  ALLDGLKSRGSVVVIGATNRPEAVDPALRRPGRFDREIYFPLPSVEDRAAILSLHTQKWP 909

Query: 481  KTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINALKRTFPLKQVLSATCDP-----HP 540
            K ++   PLL WIA++T+GFAGADLQALCTQ A++ALKR FPLK+VLSA+ +       P
Sbjct: 910  KPIDG--PLLQWIARRTAGFAGADLQALCTQAAMSALKRNFPLKEVLSASGEQVSRVNRP 969

Query: 541  TQLPVVVEDKDWLEALSTCPPPCSRREALAALNETASSPLPIHLIPCLIQPLSTLLVSLY 600
                ++VE++DWLEAL   PPPCSRREA  A N+  SSPLP HLIPCL+QPLSTLLVSLY
Sbjct: 970  PLPSILVEERDWLEALLYSPPPCSRREAGMAANDVPSSPLPFHLIPCLLQPLSTLLVSLY 1029

Query: 601  FEESICLPKTLLK-----------ALERK---STKWWFHLNNFNQDSEI--RIKRKWQGS 660
             +E I LP  LLK           AL+ K   ++ WW H+++F QD++I   I+ K QGS
Sbjct: 1030 LDERITLPTNLLKAATLIKSVIVSALDGKKIVTSCWWSHVHDFVQDADIANEIEIKLQGS 1089

Query: 661  GANILEHSSSVNSGKFISEEDTSTTDHEN-----------SNQTSFDLRNSFGFRIMIAG 720
            G  ++E S+  +SG    +    ++  EN              +SF L N  GFRI+IAG
Sbjct: 1090 GV-LVEDSTFGSSGVLNVDTSNESSKFENLGHCGGRPSTMVEHSSFTLGNKSGFRILIAG 1149

Query: 721  NPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-GYATLGQGIAYAFDNTFLRESCLIFM 780
            NPRSG RHLASC+++C+++HV +RKVD+ATISQ G+  L QGI+    N     SCL+FM
Sbjct: 1150 NPRSGPRHLASCLIHCYIQHVEVRKVDIATISQEGHGDLVQGISQILLNCSSMGSCLVFM 1209

Query: 781  PR---------------------------------------------------------- 840
            PR                                                          
Sbjct: 1210 PRIDLWAIETQSQTSEECGLYLNEDQYLEDGTIVNDDDQLGERENRCYSDQSKSTERTGL 1269

Query: 841  -----------------------------ATSEIPFLQLPREIRHFFQNDVSNCKMLGAR 900
                                         ATSE+PFL LP+EIR FF+ND+S C+   + 
Sbjct: 1270 QDECLSSASYAWSSFVEQVESLSTPLMILATSEVPFLLLPQEIRQFFRNDLSMCRPTTSE 1329

Query: 901  AHKIPRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKLLVLLIHQNNHVRDTNACNSYNPP 960
             H +PRFS+QI  DG  DH+++INQSAA+LS D++KLLV LIHQ +H R T  C  Y  P
Sbjct: 1330 -HSVPRFSVQI--DGVFDHDMVINQSAAELSRDIVKLLVHLIHQKSHTR-TLTCTKYQIP 1389

Query: 961  ----------SDFIEDQAPKHK------------------------HTNLNSEISNVGYK 999
                          ++ A +H                          +NL S IS  G++
Sbjct: 1390 VIQDENNAENQQIDKETASEHNGEMKSPDVSSLRIAPLPGSRTMKVKSNLISVISTFGHQ 1449

BLAST of PI0001227 vs. ExPASy TrEMBL
Match: A0A5D3CIS0 (Tat-binding-7-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001060 PE=4 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 1.4e-201
Identity = 504/1442 (34.95%), Postives = 678/1442 (47.02%), Query Frame = 0

Query: 38   EWRHCELCGGGSDGKPPILMEHNNND--------------------DEFGDEDGWLGHLV 97
            E R C LCGGG DGKPP  M  ++ +                    D FGDE GWLG L+
Sbjct: 415  EGRRCGLCGGGIDGKPPKKMAQDSGESGNEACSGSSASEEPNYDKWDGFGDEPGWLGRLL 474

Query: 98   GPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLKCTHCKRRGATLGC 157
            GPI D   I   W+H  C +WSP V+F     LKNV+AAL RG++LKCT C R GAT+GC
Sbjct: 475  GPINDRYGIAGIWVHQHCAVWSPEVYFAGLGCLKNVRAALCRGRALKCTRCGRPGATIGC 534

Query: 158  RLEQCQKTYHLVRSK-----FDHKQFLMACTDHGHIFQLETHLN---------SPEKIEF 217
            R+++C KTYHL  ++     FDH++FL+ACTDH HIFQ   H N           +K++ 
Sbjct: 535  RVDRCPKTYHLPCARANGCIFDHRKFLIACTDHRHIFQ--PHGNQYLARIKRLKAKKMKL 594

Query: 218  KMGKQYSKEDHKCIDKDDD--SNQEDDQQ--------------EMQPIFIGGTATHSNSN 277
            ++ KQ +    + I+ ++    N  +D++               + P++IGG        
Sbjct: 595  EIKKQSNDAWRRDIEAEEKWLENCGEDEEFLKRESKRLHRDLVRIAPVYIGG-------- 654

Query: 278  NNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELF--------RGLAI-------- 337
            +N + E +  GWESVAGLQ VIQCMKE V LPLLYPELF        RG+ +        
Sbjct: 655  SNSEGENLFHGWESVAGLQGVIQCMKEVVFLPLLYPELFDRFGITPPRGVLLHGYPGTGK 714

Query: 338  THVVRALVASCARANKIVAYFSRKGADCLGKNVNS------------------------- 397
            THVVRAL+ SCAR +K +AYF+RKGADCLGK V                           
Sbjct: 715  THVVRALIGSCARGDKRIAYFARKGADCLGKYVGDAERQLRLLFQVAERCQPSIIFFDEI 774

Query: 398  --------------------------------------------NVIDPALRRPGRFDRE 457
                                                          +DPALRRPGRFDRE
Sbjct: 775  DGLAPCRTRQQDQTHNSVVSTLLALLDGLKSRGSVVVIGATNRPEAVDPALRRPGRFDRE 834

Query: 458  IYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINAL 517
            IYFPLPS EDR +ILSL+TQKWPK ++   PLL WIA++T+GFAGADLQALCTQ A++AL
Sbjct: 835  IYFPLPSVEDRAAILSLHTQKWPKPIDG--PLLQWIARRTAGFAGADLQALCTQAAMSAL 894

Query: 518  KRTFPLKQVLSATCDP-----HPTQLPVVVEDKDWLEALSTCPPPCSRREALAALNETAS 577
            KR FPLK+VLSA+ +       P    ++VE++DWLEAL   PPPCSRREA  A N+ AS
Sbjct: 895  KRNFPLKEVLSASGEQVLRVNRPPLPSILVEERDWLEALLYSPPPCSRREAGMAANDVAS 954

Query: 578  SPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLK-----------ALERK---STKWW 637
            SPLP HLIPCL+QPLSTLLVSLY +E I LP  LLK           AL+ K   ++ WW
Sbjct: 955  SPLPFHLIPCLLQPLSTLLVSLYLDERITLPTNLLKAATSIKSVIVSALDGKNIVTSCWW 1014

Query: 638  FHLNNFNQDSEI--RIKRKWQGSGANILEHSSSVNSGKFISEEDTSTTDHEN-------- 697
             H+++F QD++I   I+ K QGSG  ++E S+  +SG    +    ++  EN        
Sbjct: 1015 SHVHDFVQDADIANEIEIKLQGSGV-LVEDSTFGSSGVLNIDTGNESSKFENLGHCGGRP 1074

Query: 698  ---SNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-GYA 757
                  TSF L N  GFRI+IAGN RSG RHLASC+++C+++HV +RKVD+ATISQ G+ 
Sbjct: 1075 ATMVEHTSFTLGNKSGFRILIAGNTRSGPRHLASCLIHCYIQHVEVRKVDIATISQEGHG 1134

Query: 758  TLGQGIAYAFDNTFLRESCLIFMPR----------------------------------- 817
             L  GI+    N     +CL+FMPR                                   
Sbjct: 1135 DLVHGISQILLNCSSMGACLVFMPRIDLWAIETQSQTSEECGFYLNEDEHVEDGIIVKDE 1194

Query: 818  ----------------------------------------------------ATSEIPFL 877
                                                                ATSE+PFL
Sbjct: 1195 GQLGERDDCRYPDQSKSSERTGLQDECLSSASYAWSSFVEQVESLSTPLMILATSEVPFL 1254

Query: 878  QLPREIRHFFQNDVSNCKMLGARAHKIPRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKL 937
             LP+EIR FF+ND+S  +   +  H +PRFS+QI  DG  DH+++INQSAA+LS D++KL
Sbjct: 1255 LLPQEIREFFRNDLSMFRPTTSE-HSVPRFSVQI--DGVFDHDMVINQSAAELSRDIVKL 1314

Query: 938  LVLLIHQNNHVRDTNACNSYNPP----------SDFIEDQAPKHK--------------- 997
            LV LIHQ +H + T+ C  Y  P              ++ A +H                
Sbjct: 1315 LVHLIHQKSHTK-TSTCTKYQIPVIQGENNAENQQIDKETASEHNGEMKSPDVSSLRIAP 1374

Query: 998  ---------HTNLNSEISNVGYKILQNPQYSNLCWVTSNFKEGPSANLSKSQWQDWKFNP 999
                      +NL S IS  G++IL+ P ++ LCWVTS  KEGP A++S   W+ W FN 
Sbjct: 1375 LPGSRTMKVKSNLISVISTFGHQILRYPHFAELCWVTSKLKEGPYADVS-GPWKGWPFNS 1434

BLAST of PI0001227 vs. NCBI nr
Match: XP_022156988.1 (uncharacterized protein LOC111023817 [Momordica charantia])

HSP 1 Score: 885.6 bits (2287), Expect = 4.2e-253
Identity = 526/1054 (49.91%), Postives = 630/1054 (59.77%), Query Frame = 0

Query: 2    MKWNHKKEGVSDDSE--------GEKLSVKRRKGMENENKKLDDE---WRHCELCGGGSD 61
            MK  HK    SDD E        G K  +K ++  + +N++++ E   WRHC LCGG + 
Sbjct: 321  MKRKHK----SDDREESRGALKLGSKRKMKTKRSEKEQNEEVEAEESSWRHCVLCGGKAK 380

Query: 62   GKPPILMEHN-----NNDDEFGDEDGWLGHLVGPIIDHNVIPRKWLHYQCVIWSPVVHFT 121
                    HN     N++D FGDEDGWLG L+GP  D N IPR W+HYQC+IWSP V+  
Sbjct: 381  LAANSHHNHNHDHDDNDNDGFGDEDGWLGRLLGPFTDSNSIPRLWVHYQCIIWSPEVYVD 440

Query: 122  ESVSLKNVQAALKRGKSLKCTHCKRRGATLGCRLEQCQKTYHL--VRS---KFDHKQFLM 181
            E+  L++V+AAL RGKSLKC+HCKRRGATLGCR+++CQKTYHL   RS   KF HKQFL+
Sbjct: 441  ETGRLRDVKAALNRGKSLKCSHCKRRGATLGCRIKECQKTYHLACARSNGCKFYHKQFLI 500

Query: 182  ACTDHGHIFQLETHLNSPEKIEFKMGKQYSKED----HKCIDKDDDSNQED----DQQEM 241
            AC DH HIF      ++ +KI+ +  K+  +      H+    DD    E+    + ++ 
Sbjct: 501  ACPDHLHIFHHPRDDSNSDKIKLRARKEKLETSNHARHEDTQTDDGGGNEEFSKQNSKKS 560

Query: 242  QPIFIGGTATHSNSNNNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELFRGLAI- 301
               F   T T+      K+E  +  GWESVAGLQ+VI+CMKE VVLPLLYPE F GL I 
Sbjct: 561  NQEFRKITPTYIGGEGEKEESHMSAGWESVAGLQHVIECMKEVVVLPLLYPEFFEGLGIV 620

Query: 302  ---------------THVVRALVASCARANKIVAYFSRKGADCLGKNVNS---------- 361
                           THVVRALV SCAR NK +AYFSRKGADCLGK V            
Sbjct: 621  PPRGVLLHGYPGTGKTHVVRALVGSCARGNKXIAYFSRKGADCLGKYVGDSEQLLRRLFQ 680

Query: 362  -----------------------------------------------------------N 421
                                                                       +
Sbjct: 681  VAQDCQPSIIFFDEIDGLAPCRTRKQDQTHTSVVSTLLALLDGLKSRGSXVVIGATNRPD 740

Query: 422  VIDPALRRPGRFDREIYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAG 481
             IDPALRRPGRFDREIYFPLPS EDRISIL LYT KWPKTL SN PLL WIA+KT GFAG
Sbjct: 741  AIDPALRRPGRFDREIYFPLPSLEDRISILKLYTHKWPKTLESNGPLLQWIARKTLGFAG 800

Query: 482  ADLQALCTQTAINALKRTFPLKQVLSATCDPH---PTQL-PVVVEDKDWLEALSTCPPPC 541
            ADLQALCTQTAI ALKRTFPLKQVL A+ D     PT L PV+VE++DWLEALS+CPPPC
Sbjct: 801  ADLQALCTQTAIIALKRTFPLKQVLHASKDTKYGPPTHLPPVIVEERDWLEALSSCPPPC 860

Query: 542  SRREALAALNETASSPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTL-----------L 601
            SRREA  A N+  SSPLP HLIPCLI+PLS LLVSLY E+ ICLP+TL           L
Sbjct: 861  SRREATMAANDVVSSPLPSHLIPCLIRPLSILLVSLYLEKCICLPQTLSKAAAIIENVML 920

Query: 602  KALERK---STKWWFHLNNFNQDSEI--RIKRKWQGSGANILEHSSSVNSGKFISEEDTS 661
             ALERK   S KWW H+++F Q+ +I   I+RKWQ    NILEHS   NS + I E    
Sbjct: 921  SALERKTXNSNKWWSHVDDFIQEDDIADEIERKWQDCRTNILEHSCLANSYESIPE---- 980

Query: 662  TTDHENSNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQG 721
                +N+  TSFDL NS GFRIMIAGNP  GQRHLASCILNCFV HV IRKVDMAT SQG
Sbjct: 981  ----DNAKYTSFDLGNSQGFRIMIAGNPSLGQRHLASCILNCFVGHVEIRKVDMATTSQG 1040

Query: 722  YATLGQGIAYAFDNTFLRESCLIFMPR--------------------------------- 781
            Y  L QGI++   N   R  CLIFMPR                                 
Sbjct: 1041 YNNLEQGISHTLANCLSRGPCLIFMPRIDLWALETHEDQTLEHDNTHPTDEQCIRKAIVH 1100

Query: 782  -------------------------ATSEIPFLQLPREIRHFFQNDVSNCKMLGARAHKI 841
                                     ATSE+PFL LP+EIR FF ND+++CK L A  HKI
Sbjct: 1101 RASSAWTLFIYQVESLSLCTPXIFLATSEVPFLLLPQEIRLFFLNDLTDCKRLVASKHKI 1160

Query: 842  PRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKLLVLLIHQNNHVRDTNACNSYNPPSDFI 863
            PRFSIQID+  T +H+L+I++SAA LS D++KLL LLIHQ  HV      +     S++ 
Sbjct: 1161 PRFSIQIDE--TFNHDLVIDRSAATLSKDMVKLLALLIHQKYHVNXNEXHSEXTRSSNYQ 1220

BLAST of PI0001227 vs. NCBI nr
Match: XP_038898386.1 (uncharacterized protein LOC120086038 [Benincasa hispida])

HSP 1 Score: 723.0 bits (1865), Expect = 3.6e-204
Identity = 507/1440 (35.21%), Postives = 682/1440 (47.36%), Query Frame = 0

Query: 38   EWRHCELCGGGSDGKPPILMEHNNNDDE--------------------FGDEDGWLGHLV 97
            E R C LCGGG DGKPP  +  ++ + E                    FGDE GWLG L+
Sbjct: 421  EGRRCGLCGGGIDGKPPKKLVQDSGESEHEACSGSSASEEPNYDKWDGFGDEPGWLGRLL 480

Query: 98   GPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLKCTHCKRRGATLGC 157
            GPI D   I   W+H  C +WSP V+F     LKNV+AAL RG++LKCT C R GAT+GC
Sbjct: 481  GPINDRYGIAGIWVHQHCAVWSPEVYFAGLGCLKNVRAALCRGRALKCTRCGRPGATIGC 540

Query: 158  RLEQCQKTYHLVRSK-----FDHKQFLMACTDHGHIFQLETHLN---------SPEKIEF 217
            R+++C KTYHL  ++     FDH++FL+ACTDH HIFQ   H N           +K++ 
Sbjct: 541  RVDRCPKTYHLPCARANGCIFDHRKFLIACTDHRHIFQ--PHGNQYLARIKRLKAKKMKL 600

Query: 218  KMGKQYSKEDHKCIDKDDD--SNQEDDQQ--------------EMQPIFIGGTATHSNSN 277
            ++ KQ +    + I+ ++    N  +D++               + P++IGG        
Sbjct: 601  EIKKQSNDAWRRDIEAEEKWLENCGEDEEFLKRESKRLHRDLVRIAPVYIGG-------- 660

Query: 278  NNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELF--------RGLAI-------- 337
            +N + E +  GWESVAGLQ VIQCMKE V LPLLYPELF        RG+ +        
Sbjct: 661  SNSEGENLFHGWESVAGLQGVIQCMKEVVFLPLLYPELFDRFGITPPRGVLLHGYPGTGK 720

Query: 338  THVVRALVASCARANKIVAYFSRKGADCLGKNVNS------------------------- 397
            THVVRAL+ SCAR +K +AYF+RKGADCLGK V                           
Sbjct: 721  THVVRALIGSCARGDKRIAYFARKGADCLGKYVGDAERQLRLLFQVAERCQPSIIFFDEI 780

Query: 398  --------------------------------------------NVIDPALRRPGRFDRE 457
                                                          +DPALRRPGRFDRE
Sbjct: 781  DGLAPCRTRQQDQTHNSVVSTLLALLDGLKSRGCVVVIGATNRPEAVDPALRRPGRFDRE 840

Query: 458  IYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINAL 517
            IYFPLPS EDR +ILSL+TQKWPK ++   PLL WIA++T+GFAGADLQALCTQ A++AL
Sbjct: 841  IYFPLPSVEDRAAILSLHTQKWPKPVDG--PLLQWIARRTAGFAGADLQALCTQAAMSAL 900

Query: 518  KRTFPLKQVLSA-----TCDPHPTQLPVVVEDKDWLEALSTCPPPCSRREALAALNETAS 577
            KR FPLK+VLSA     + D  P    ++VE++DWLEAL   PPPCSRREA  A N+ AS
Sbjct: 901  KRNFPLKEVLSASGEQVSRDNRPPLPSILVEERDWLEALLYSPPPCSRREAGMAANDVAS 960

Query: 578  SPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLK-----------ALERK---STKWW 637
            SPLP+HLIPCL++PLSTLL+SLY +E I LP  L K           AL+ +   ++ WW
Sbjct: 961  SPLPLHLIPCLLRPLSTLLLSLYLDERIRLPTNLFKAATLIKSVIVSALDGRRIVTSCWW 1020

Query: 638  FHLNNFNQDSEI--RIKRKWQGS----------GANILEHSSSVNSGKFISEEDTSTTDH 697
             H+ +F Q+++I   I+ K QGS          G+ +L + +S  S KF +    S    
Sbjct: 1021 SHVRDFVQEADIANEIETKLQGSGVLLEDSTFGGSVVLNNDTSNESSKFENVGHRSGPPT 1080

Query: 698  ENSNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-GYAT 757
                QTSF L N  GFRI+IAGNPRSG RHLASC+L+C+++HV IRKVD+ATI Q G+  
Sbjct: 1081 TMVEQTSFTLGNKSGFRILIAGNPRSGPRHLASCLLHCYIQHVEIRKVDIATILQEGHGD 1140

Query: 758  LGQGIAYAFDNTFLRESCLIFMPR------------------------------------ 817
            L QGI+    N     SCL+FMPR                                    
Sbjct: 1141 LVQGISQILLNCSSMGSCLVFMPRIDLWAIETQSQTSEECGFYPNGDQYPEDGIIVKDDG 1200

Query: 818  ---------------------------------------------------ATSEIPFLQ 877
                                                               ATSE+PFL 
Sbjct: 1201 HLGGRENHRYSDQSKSTERTGLQDESLSSSSYAWSSFVEQVESLSTPSMILATSEVPFLL 1260

Query: 878  LPREIRHFFQNDVSNCKMLGARAHKIPRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKLL 937
            LP+EIR FF+ND+S C+   +  H +PRFS+QI  DG  DH+++INQSAA+LS D++KLL
Sbjct: 1261 LPQEIRQFFRNDLSICRPTTSE-HSVPRFSVQI--DGVFDHDMVINQSAAELSRDIVKLL 1320

Query: 938  VLLIHQNNHVR--------------DTNACNSYNPPSDFIEDQAPKHKH----------- 997
            V LIHQ +H +              ++NA N      +  E    K  H           
Sbjct: 1321 VHLIHQKSHTKSSTCTKNLISVVQNESNAANQQIDKENASEHNGEKKSHDVSSVRIAPLP 1380

BLAST of PI0001227 vs. NCBI nr
Match: KAG6577006.1 (hypothetical protein SDJN03_24580, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 721.8 bits (1862), Expect = 8.0e-204
Identity = 455/1047 (43.46%), Postives = 596/1047 (56.92%), Query Frame = 0

Query: 38   EWRHCELCGGGSDGKPPILMEHNNNDDE--------------------FGDEDGWLGHLV 97
            E R C LCGGG DGKPP  +  ++ + E                    FGDE GWLG L+
Sbjct: 399  EGRRCGLCGGGIDGKPPKKLVQDSGESENEACSGSSASEEPNYDKWDGFGDEPGWLGRLL 458

Query: 98   GPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLKCTHCKRRGATLGC 157
            GPI D   I   W+H  C +WSP V+F     LKNV+AAL RG++LKCT C R GAT+GC
Sbjct: 459  GPINDRYGIAGIWVHQHCAVWSPEVYFAGLGCLKNVRAALCRGRALKCTRCGRPGATIGC 518

Query: 158  RLEQCQKTYHLVRSK-----FDHKQFLMACTDHGHIFQLETHLN---------SPEKIEF 217
            R+++C +TYHL  ++     FDH++FL+ACTDH HIFQ   H N           +K++ 
Sbjct: 519  RVDRCPRTYHLPCARANGCIFDHRKFLIACTDHRHIFQ--PHGNQYLARIKRLKAKKMKL 578

Query: 218  KMGKQYSKEDHKCIDKDDD--SNQEDDQQ--------------EMQPIFIGGTATHSNSN 277
            ++ KQ +    + I+ ++    N  +D++               + P++IGG        
Sbjct: 579  EIKKQSNDAWRRDIEAEEKWLENCGEDEEFLKRESKRLHRDLVRIAPVYIGG-------- 638

Query: 278  NNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELF--------RGLAI-------- 337
            +N + E +  GWESVAGLQ VIQCMKE V LPLLYPELF        RG+ +        
Sbjct: 639  SNSEGENLYRGWESVAGLQGVIQCMKEVVFLPLLYPELFDRFGITPPRGVLLHGYPGTGK 698

Query: 338  THVVRALVASCARANKIVAYFSRKGADCLGKNVNS------------------------- 397
            THVVRAL+ SCAR +K +AYF+RKGADCLGK V                           
Sbjct: 699  THVVRALIGSCARGDKRIAYFARKGADCLGKYVGDAERQLRLLFQVAEKCQPSIIFFDEI 758

Query: 398  --------------------------------------------NVIDPALRRPGRFDRE 457
                                                          +DPALRRPGRFDRE
Sbjct: 759  DGLAPCRTRQQDQTHNSVVSTLLALLDGLKSRGSVVVIGATNRPEAVDPALRRPGRFDRE 818

Query: 458  IYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINAL 517
            IYFPLPS EDR +ILSL+TQ+WPK +    PLL WIA++T+GFAGADLQALCTQ A++AL
Sbjct: 819  IYFPLPSVEDRAAILSLHTQRWPKPVEG--PLLQWIARRTAGFAGADLQALCTQAAMSAL 878

Query: 518  KRTFPLKQVLSAT--------CDPHPTQLPVVVEDKDWLEALSTCPPPCSRREALAALNE 577
            KR FPLK+VLSA+        C P P+   ++VE++DWLEAL   PPPCSRREA  A N+
Sbjct: 879  KRNFPLKKVLSASGEQASRDNCPPLPS---ILVEERDWLEALLYSPPPCSRREAGMAAND 938

Query: 578  TASSPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLKALE------------RK--ST 637
             ASSPLP HLIPCL+QPLS LLVSLY +E I LP  LLKA              RK  ++
Sbjct: 939  VASSPLPSHLIPCLLQPLSALLVSLYLDERISLPANLLKAATLIKSVIVTALDGRKIVTS 998

Query: 638  KWWFHLNNFNQDSEI--RIKRKWQGSGANILEHSSSVNSGKFISEEDTSTTDHENSN--- 697
             WW ++++F QD++I   I+ K QGSG  +LE S+ V SG   +E    ++  E+     
Sbjct: 999  CWWSYVHDFLQDADIANEIEIKLQGSGV-LLEESTFVGSGVLNNETGNDSSKFESVGHRG 1058

Query: 698  -------QTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-G 757
                   ++ F L N  GFRI+IAGNPRSG RHLASC+L+C+++HV IRKVD+ATISQ G
Sbjct: 1059 GPPTTMVESPFTLENKSGFRILIAGNPRSGPRHLASCLLHCYIQHVEIRKVDIATISQEG 1118

Query: 758  YATLGQGIAYAFDNTFLR------ESCLIFMPRATSEIPFLQLPREIRHFFQNDVSNCKM 817
            +  L QGI+       +       ES +  +  ATSE+PFL LP+EIR FF+ND+S C+ 
Sbjct: 1119 HGDLVQGISQILRLDLVALVLSYGESVMYLL--ATSEVPFLLLPQEIRRFFRNDLSICRP 1178

Query: 818  LGARAHKIPRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKLLVLLIHQNNHVR------- 870
                 H +PRFS+QI  DG  DH+++INQSA +LS D+LKLLV LIHQ  H +       
Sbjct: 1179 T-TTEHSVPRFSVQI--DGGFDHDMVINQSATELSRDILKLLVHLIHQKAHTKTSICTKN 1238

BLAST of PI0001227 vs. NCBI nr
Match: KAG6577006.1 (hypothetical protein SDJN03_24580, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 84.0 bits (206), Expect = 8.4e-12
Identity = 42/121 (34.71%), Postives = 70/121 (57.85%), Query Frame = 0

Query: 877  HGTFAKIDISSLPTTSTCSINCCRDCLNILHIVAKEIVRHEFGSKRFDWSTESIHETVMS 936
            H + ++ D+S+  +   CSI CC  CLNIL+   + I+++E GS   +W+ E +H+ V +
Sbjct: 1617 HSSPSRSDLST-SSAQVCSIRCCTGCLNILYGATRTILQNELGSNWNNWTAEDVHDIVAA 1676

Query: 937  MSVNLLTGVRKLFVDGKGCHSFDDIERRENYDKLLESYSLASCSCKISRNVIVMPLECSY 996
            +SV+LL  VR+ F+DG     F+D  RR+  D         +C CK  ++++ M +EC  
Sbjct: 1677 LSVDLLAAVRRAFLDGNDTRVFND--RRKASDS-------RTCDCKSWKDMVFMAVECIC 1727

Query: 997  H 998
            H
Sbjct: 1737 H 1727

HSP 2 Score: 714.9 bits (1844), Expect = 9.7e-202
Identity = 505/1443 (35.00%), Postives = 679/1443 (47.05%), Query Frame = 0

Query: 38   EWRHCELCGGGSDGKPPILMEHNNND--------------------DEFGDEDGWLGHLV 97
            E R C LCGGG DGKPP  M  ++ +                    D FGDE GWLG L+
Sbjct: 411  EGRRCGLCGGGIDGKPPKKMAQDSGESGNEACSGSSASEEPNYDKWDGFGDEPGWLGRLL 470

Query: 98   GPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLKCTHCKRRGATLGC 157
            GPI D   I   W+H  C +WSP V+F     LKNV+AAL RG++LKCT C R GAT+GC
Sbjct: 471  GPINDRYGIAGIWVHQHCAVWSPEVYFAGLGCLKNVRAALCRGRALKCTRCGRPGATIGC 530

Query: 158  RLEQCQKTYHLVRSK-----FDHKQFLMACTDHGHIFQLETHLN---------SPEKIEF 217
            R+++C KTYHL  ++     FDH++FL+ACTDH HIFQ   H N           +K++ 
Sbjct: 531  RVDRCPKTYHLPCARANGCIFDHRKFLIACTDHRHIFQ--PHGNQYLARIKRLKAKKMKL 590

Query: 218  KMGKQYSKEDHKCIDKDDD--SNQEDDQQ--------------EMQPIFIGGTATHSNSN 277
            ++ KQ +    + I+ ++    N  +D++               + P++IGG        
Sbjct: 591  EIKKQSNDAWRRDIEAEEKWLENCGEDEEFLKRESKRLHRDLVRIAPVYIGG-------- 650

Query: 278  NNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELF--------RGLAI-------- 337
            +N + E +  GWESVAGLQ VIQCMKE V LPLLYPELF        RG+ +        
Sbjct: 651  SNSEGENLFHGWESVAGLQGVIQCMKEVVFLPLLYPELFDRFGITPPRGVLLHGYPGTGK 710

Query: 338  THVVRALVASCARANKIVAYFSRKGADCLGKNVNS------------------------- 397
            THVVRAL+ SCAR +K +AYF+RKGADCLGK V                           
Sbjct: 711  THVVRALIGSCARGDKRIAYFARKGADCLGKYVGDAERQLRLLFQVAERCQPSIIFFDEI 770

Query: 398  --------------------------------------------NVIDPALRRPGRFDRE 457
                                                          +DPALRRPGRFDRE
Sbjct: 771  DGLAPCRTRQQDQTHNSVVSTLLALLDGLKSRGSVVVIGATNRPEAVDPALRRPGRFDRE 830

Query: 458  IYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINAL 517
            IYFPLPS EDR +ILSL+TQKWPK ++   PLL WIA++T+GFAGADLQALCTQ A++AL
Sbjct: 831  IYFPLPSVEDRAAILSLHTQKWPKPIDG--PLLQWIARRTAGFAGADLQALCTQAAMSAL 890

Query: 518  KRTFPLKQVLSATCDP-----HPTQLPVVVEDKDWLEALSTCPPPCSRREALAALNETAS 577
            KR FPLK+VLSA+ +       P    ++VE++DWLEAL   PPPCSRREA  A N+ AS
Sbjct: 891  KRNFPLKEVLSASGEQVLRVNRPPLPSILVEERDWLEALLYSPPPCSRREAGMAANDVAS 950

Query: 578  SPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLK-----------ALERK---STKWW 637
            SPLP HLIPCL+QPLSTLLVSLY +E I LP  LLK           AL+ K   ++ WW
Sbjct: 951  SPLPFHLIPCLLQPLSTLLVSLYLDERITLPTNLLKAATSIKSVIVSALDGKNIVTSCWW 1010

Query: 638  FHLNNFNQDSEI--RIKRKWQGSGANILEHSSSVNSGKFISEEDTSTTDHEN-------- 697
             H+++F QD++I   I+ K QGSG  ++E S+  +SG    +    ++  EN        
Sbjct: 1011 SHVHDFVQDADIANEIEIKLQGSGV-LVEDSTFGSSGVLNIDTGNESSKFENLGHCGGRP 1070

Query: 698  ---SNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-GYA 757
                  TSF L N  GFRI+IAGN RSG RHLASC+++C+++HV +RKVD+ATISQ G+ 
Sbjct: 1071 ATMVEHTSFTLGNKSGFRILIAGNTRSGPRHLASCLIHCYIQHVEVRKVDIATISQEGHG 1130

Query: 758  TLGQGIAYAFDNTFLRESCLIFMPR----------------------------------- 817
             L  GI+    N     +CL+FMPR                                   
Sbjct: 1131 DLVHGISQILLNCSSMGACLVFMPRIDLWAIETQSQTSEECGFYLNEDEHVEDGIIVKDE 1190

Query: 818  ----------------------------------------------------ATSEIPFL 877
                                                                ATSE+PFL
Sbjct: 1191 GQLGERDDCRYPDQSKSSERTGLQDECLSSASYAWGSFVEQVESLSTPLMILATSEVPFL 1250

Query: 878  QLPREIRHFFQNDVSNCKMLGARAHKIPRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKL 937
             LP+EIR FF+ND+S C+   +  H +PRFS+QI  DG  DH+++INQSAA+LS D++KL
Sbjct: 1251 LLPQEIREFFRNDLSMCRPTTSE-HSVPRFSVQI--DGVFDHDMVINQSAAELSRDIVKL 1310

Query: 938  LVLLIHQNNHVRDTNACNSYNPP----------SDFIEDQAPKHK--------------- 997
            LV LIHQ +H + T+ C  Y  P              ++ A +H                
Sbjct: 1311 LVHLIHQKSHTK-TSTCTKYQIPVIQGENNAENQQIDKETASEHNGEMKSPDVSSLRIAP 1370

Query: 998  ---------HTNLNSEISNVGYKILQNPQYSNLCWVTSNFKEGPSANLSKSQWQDWKFNP 999
                      +NL S IS  G++IL+ P ++ LCWVTS  KEGP A++S   W+ W FN 
Sbjct: 1371 LPGSRTMKVKSNLISVISTFGHQILRYPHFAELCWVTSKLKEGPYADVS-GPWKGWPFNS 1430

BLAST of PI0001227 vs. NCBI nr
Match: XP_008456208.1 (PREDICTED: uncharacterized protein LOC103496212 [Cucumis melo])

HSP 1 Score: 714.9 bits (1844), Expect = 9.7e-202
Identity = 505/1443 (35.00%), Postives = 679/1443 (47.05%), Query Frame = 0

Query: 38   EWRHCELCGGGSDGKPPILMEHNNND--------------------DEFGDEDGWLGHLV 97
            E R C LCGGG DGKPP  M  ++ +                    D FGDE GWLG L+
Sbjct: 476  EGRRCGLCGGGIDGKPPKKMAQDSGESGNEACSGSSASEEPNYDKWDGFGDEPGWLGRLL 535

Query: 98   GPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLKCTHCKRRGATLGC 157
            GPI D   I   W+H  C +WSP V+F     LKNV+AAL RG++LKCT C R GAT+GC
Sbjct: 536  GPINDRYGIAGIWVHQHCAVWSPEVYFAGLGCLKNVRAALCRGRALKCTRCGRPGATIGC 595

Query: 158  RLEQCQKTYHLVRSK-----FDHKQFLMACTDHGHIFQLETHLN---------SPEKIEF 217
            R+++C KTYHL  ++     FDH++FL+ACTDH HIFQ   H N           +K++ 
Sbjct: 596  RVDRCPKTYHLPCARANGCIFDHRKFLIACTDHRHIFQ--PHGNQYLARIKRLKAKKMKL 655

Query: 218  KMGKQYSKEDHKCIDKDDD--SNQEDDQQ--------------EMQPIFIGGTATHSNSN 277
            ++ KQ +    + I+ ++    N  +D++               + P++IGG        
Sbjct: 656  EIKKQSNDAWRRDIEAEEKWLENCGEDEEFLKRESKRLHRDLVRIAPVYIGG-------- 715

Query: 278  NNKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELF--------RGLAI-------- 337
            +N + E +  GWESVAGLQ VIQCMKE V LPLLYPELF        RG+ +        
Sbjct: 716  SNSEGENLFHGWESVAGLQGVIQCMKEVVFLPLLYPELFDRFGITPPRGVLLHGYPGTGK 775

Query: 338  THVVRALVASCARANKIVAYFSRKGADCLGKNVNS------------------------- 397
            THVVRAL+ SCAR +K +AYF+RKGADCLGK V                           
Sbjct: 776  THVVRALIGSCARGDKRIAYFARKGADCLGKYVGDAERQLRLLFQVAERCQPSIIFFDEI 835

Query: 398  --------------------------------------------NVIDPALRRPGRFDRE 457
                                                          +DPALRRPGRFDRE
Sbjct: 836  DGLAPCRTRQQDQTHNSVVSTLLALLDGLKSRGSVVVIGATNRPEAVDPALRRPGRFDRE 895

Query: 458  IYFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINAL 517
            IYFPLPS EDR +ILSL+TQKWPK ++   PLL WIA++T+GFAGADLQALCTQ A++AL
Sbjct: 896  IYFPLPSVEDRAAILSLHTQKWPKPIDG--PLLQWIARRTAGFAGADLQALCTQAAMSAL 955

Query: 518  KRTFPLKQVLSATCDP-----HPTQLPVVVEDKDWLEALSTCPPPCSRREALAALNETAS 577
            KR FPLK+VLSA+ +       P    ++VE++DWLEAL   PPPCSRREA  A N+ AS
Sbjct: 956  KRNFPLKEVLSASGEQVLRVNRPPLPSILVEERDWLEALLYSPPPCSRREAGMAANDVAS 1015

Query: 578  SPLPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLK-----------ALERK---STKWW 637
            SPLP HLIPCL+QPLSTLLVSLY +E I LP  LLK           AL+ K   ++ WW
Sbjct: 1016 SPLPFHLIPCLLQPLSTLLVSLYLDERITLPTNLLKAATSIKSVIVSALDGKNIVTSCWW 1075

Query: 638  FHLNNFNQDSEI--RIKRKWQGSGANILEHSSSVNSGKFISEEDTSTTDHEN-------- 697
             H+++F QD++I   I+ K QGSG  ++E S+  +SG    +    ++  EN        
Sbjct: 1076 SHVHDFVQDADIANEIEIKLQGSGV-LVEDSTFGSSGVLNIDTGNESSKFENLGHCGGRP 1135

Query: 698  ---SNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-GYA 757
                  TSF L N  GFRI+IAGN RSG RHLASC+++C+++HV +RKVD+ATISQ G+ 
Sbjct: 1136 ATMVEHTSFTLGNKSGFRILIAGNTRSGPRHLASCLIHCYIQHVEVRKVDIATISQEGHG 1195

Query: 758  TLGQGIAYAFDNTFLRESCLIFMPR----------------------------------- 817
             L  GI+    N     +CL+FMPR                                   
Sbjct: 1196 DLVHGISQILLNCSSMGACLVFMPRIDLWAIETQSQTSEECGFYLNEDEHVEDGIIVKDE 1255

Query: 818  ----------------------------------------------------ATSEIPFL 877
                                                                ATSE+PFL
Sbjct: 1256 GQLGERDDCRYPDQSKSSERTGLQDECLSSASYAWGSFVEQVESLSTPLMILATSEVPFL 1315

Query: 878  QLPREIRHFFQNDVSNCKMLGARAHKIPRFSIQIDDDGTSDHNLLINQSAAKLSNDLLKL 937
             LP+EIR FF+ND+S C+   +  H +PRFS+QI  DG  DH+++INQSAA+LS D++KL
Sbjct: 1316 LLPQEIREFFRNDLSMCRPTTSE-HSVPRFSVQI--DGVFDHDMVINQSAAELSRDIVKL 1375

Query: 938  LVLLIHQNNHVRDTNACNSYNPP----------SDFIEDQAPKHK--------------- 997
            LV LIHQ +H + T+ C  Y  P              ++ A +H                
Sbjct: 1376 LVHLIHQKSHTK-TSTCTKYQIPVIQGENNAENQQIDKETASEHNGEMKSPDVSSLRIAP 1435

Query: 998  ---------HTNLNSEISNVGYKILQNPQYSNLCWVTSNFKEGPSANLSKSQWQDWKFNP 999
                      +NL S IS  G++IL+ P ++ LCWVTS  KEGP A++S   W+ W FN 
Sbjct: 1436 LPGSRTMKVKSNLISVISTFGHQILRYPHFAELCWVTSKLKEGPYADVS-GPWKGWPFNS 1495

BLAST of PI0001227 vs. TAIR 10
Match: AT3G15120.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 534.3 bits (1375), Expect = 2.2e-151
Identity = 380/1107 (34.33%), Postives = 537/1107 (48.51%), Query Frame = 0

Query: 33   KKLDDEWRHCELCGGGSDGKPPILMEHNNND--------------------DEFGDEDGW 92
            K L  + R C LCG G+DGK P  +  +N D                    D FGD+ GW
Sbjct: 482  KPLFKQTRRCGLCGVGTDGKLPKKLMQDNGDSDVEAPSGSSSSEEQKYDILDGFGDDPGW 541

Query: 93   LGHLVGPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLKCTHCKRRG 152
            LG L+GPI D   I   W+H  C +WSP V+F     LKN++AAL RG+SLKCT C R G
Sbjct: 542  LGRLLGPINDRYGISGTWVHQNCAVWSPEVYFAGVGCLKNIRAALFRGRSLKCTRCDRPG 601

Query: 153  ATLGCRLEQCQKTYHLVRSKFDHKQFLMACTDHGHIFQ---------------------L 212
            AT GCR   C +    +   FDH++FL+ACTDH H FQ                     +
Sbjct: 602  ATTGCR--PCARANGCI---FDHRKFLIACTDHRHHFQPHGRQCQVRMTKMKTKRMRLEM 661

Query: 213  ETHLNSPEKIEFKMGKQYSK---EDHKCIDKDDDSNQEDDQQEMQPIFIGGTATHSNSNN 272
            + H N   + + +  +++ +   ED + + K +      D   + P +IGG    S+S +
Sbjct: 662  KKHSNDAWRKDVEAEEKWFEKCGEDEEFL-KRESKRLHRDLLRVAPEYIGG----SDSES 721

Query: 273  NKKEELVMGGWESVAGLQNVIQCMKEAVVLPLLYPELFRGLAITH--------------- 332
             K  E    GW+SVAGL+ V QCMKE V++PLLYPE F  L +T                
Sbjct: 722  GKAFE----GWDSVAGLEGVTQCMKEVVLIPLLYPEFFDNLGLTPPRGILLHGHPGTGKT 781

Query: 333  -VVRALVASCARANKIVAYFSRKGADCLGKNVNS-------------------------- 392
             VVRAL+ S AR N+ +AYF+RKGADCLGK V                            
Sbjct: 782  LVVRALIGSLARGNRRIAYFARKGADCLGKYVGDAERQLRLLFQVAEKCQPSIIFFDEID 841

Query: 393  -------------------------------------------NVIDPALRRPGRFDREI 452
                                                       + IDPALRRPGRFDREI
Sbjct: 842  GLAPKRSRQQDQTHSSVVSTLLALLDGLKSRGSVVVIGATNYPDAIDPALRRPGRFDREI 901

Query: 453  YFPLPSFEDRISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINALK 512
            YFPLPS +DR +I+SL+T+KWPK ++    LL WIAK+T+GFAGAD+QALCTQ A+ AL 
Sbjct: 902  YFPLPSVDDRAAIISLHTRKWPKPVSGY--LLKWIAKETAGFAGADIQALCTQAAMIALN 961

Query: 513  RTFPLKQVLSA----TCDPHPTQLP-VVVEDKDWLEALSTCPPPCSRREALAALNETASS 572
            R+FPL++ L+A        +   LP   VE++DWLEALS  PPPCSRR A  A ++  SS
Sbjct: 962  RSFPLQESLAAAELGVSSSNRAALPSFSVEERDWLEALSRSPPPCSRRGAGIAASDIFSS 1021

Query: 573  PLPIHLIPCLIQPLSTLLVSLYFEESICLPKTLLKAL------------ERKSTK--WWF 632
            PLP +L+P L+ PL +LLV+L+ +E I LP  L KA             ++K T+  WW 
Sbjct: 1022 PLPTYLVPSLLPPLCSLLVALHLDERIFLPPLLSKAAVDVQNVIRSALSDKKITEGCWWS 1081

Query: 633  HLNNFNQDSEIRIKRKWQGSGANILE---------------HSSSVNSGKFISEEDTSTT 692
            H++    + ++      + S   IL+                  S+ S KF+        
Sbjct: 1082 HVDTLLHEVDVVKDIVQRLSCTGILDGGCDLVGSVASIPGTGDCSLGSAKFMVPR---VC 1141

Query: 693  DHEN-SNQTSFDLRNSFGFRIMIAGNPRSGQRHLASCILNCFVEHVVIRKVDMATISQ-G 752
             H       S +  +  GF+++IAG P+SGQRHLASC+L+CF+ +  + K+D ATISQ G
Sbjct: 1142 RHPGVLGNASVESTSKSGFQLLIAGGPKSGQRHLASCVLHCFIGNAEMLKIDTATISQEG 1201

Query: 753  YATLGQGIAYAFDNTFLRESCLIFMPR--------------------------------- 812
               L  G+ +       ++SC++FMPR                                 
Sbjct: 1202 NGDLVLGVTHLLIKCASKKSCVVFMPRVDLWAVKTETPLNEEVECDDDSVQENCSEMGEE 1261

Query: 813  --------------------------------ATSEIPFLQLPREIRHFFQNDVS-NCKM 864
                                            ATS +P+  LP +I+ FF+ D+S  C+ 
Sbjct: 1262 KALQNGVRVSHAWNTFFEQVETLRVSTKMMILATSGMPYKLLPPKIQQFFKTDLSKECQP 1321

HSP 2 Score: 51.6 bits (122), Expect = 4.3e-06
Identity = 33/113 (29.20%), Postives = 54/113 (47.79%), Query Frame = 0

Query: 894  CSINCCRDCLNILHIVAKEIVRHEFGSKRFDWSTESIHETVMSMSVNLLTGVRKLFVDGK 953
            C   CC  C++IL     ++V  E    R   +TE IH+ V S+SV L++ VRK F+  K
Sbjct: 1790 CLYRCCSQCVSILQDSMHKLVTRELRLGRSSITTEGIHDAVSSLSVELISAVRK-FISVK 1849

Query: 954  GCHSFDDIERRENYDKLLESYSLASCSCKISRNVIVMPLECSYH---LKGHLD 1004
               +  + + +++     E     +C CK      +  +EC  H   ++G LD
Sbjct: 1850 NNGTMQEAKVKDHE----ECPENEACFCKRLSGNFLASVECCSHSAEMQGSLD 1897

BLAST of PI0001227 vs. TAIR 10
Match: AT1G05910.1 (cell division cycle protein 48-related / CDC48-related )

HSP 1 Score: 95.5 bits (236), Expect = 2.6e-19
Identity = 89/314 (28.34%), Postives = 126/314 (40.13%), Query Frame = 0

Query: 239 WESVAGLQNVIQCMKEAVVLPLLYPELFRGLAITH----------------VVRALVASC 298
           ++ + GL   I  +KE V  PLLYPE F   +IT                 + RAL  + 
Sbjct: 380 FDDIGGLSEYINDLKEMVFFPLLYPEFFASYSITPPRGVLLCGPPGTGKTLIARALACAA 439

Query: 299 ARANKIVAYFSRKGADCLGKNVNS------------------------------------ 358
           ++A + V+++ RKGAD L K V                                      
Sbjct: 440 SKAGQKVSFYMRKGADVLSKWVGEAERQLKLLFEEAQRNQPSIIFFDEIDGLAPVRSSKQ 499

Query: 359 ---------------------------------NVIDPALRRPGRFDREIYFPLPSFEDR 418
                                            + ID ALRRPGRFDRE  F LP  E R
Sbjct: 500 EQIHNSIVSTLLALMDGLDSRGQVVLIGATNRVDAIDGALRRPGRFDREFNFSLPGCEAR 559

Query: 419 ISILSLYTQKWPKTLNSNQPLLHWIAKKTSGFAGADLQALCTQTAINALKRTFPLKQVLS 465
             IL ++T+KW       + L   +A    G+ GADL+ALCT+ AI A +  +P  QV +
Sbjct: 560 AEILDIHTRKWKHP--PTRELKEELAATCVGYCGADLKALCTEAAIRAFREKYP--QVYT 619

BLAST of PI0001227 vs. TAIR 10
Match: AT1G04020.2 (breast cancer associated RING 1 )

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 49/170 (28.82%), Postives = 79/170 (46.47%), Query Frame = 0

Query: 5   NHKKEGVSDDSEGEKLSVKRRKGMENENKKLDDEWRHCELCGGGSDGKPPILMEHNNNDD 64
           N K    SDD   EK+S    K +E  +  +      C  C      +    M H +   
Sbjct: 304 NGKCSANSDDQLSEKIS----KALEQTSSNI----TICGFCQSARVSEATGEMLHYSRGR 363

Query: 65  EFGDEDGWLGHLVGPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLK 124
               +D         I   NVI    +H  C+ W+P V++ E  ++KN++A L RG  +K
Sbjct: 364 PVDGDD---------IFRSNVI---HVHSACIEWAPQVYY-EGDTVKNLKAELARGMKIK 423

Query: 125 CTHCKRRGATLGCRLEQCQKTYH------LVRSKFDHKQFLMACTDHGHI 169
           CT C  +GA LGC ++ C+++YH      + R ++D++ FL+ C  H  +
Sbjct: 424 CTKCSLKGAALGCFVKSCRRSYHVPCAREISRCRWDYEDFLLLCPAHSSV 452

BLAST of PI0001227 vs. TAIR 10
Match: AT1G04020.1 (breast cancer associated RING 1 )

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 49/170 (28.82%), Postives = 79/170 (46.47%), Query Frame = 0

Query: 5   NHKKEGVSDDSEGEKLSVKRRKGMENENKKLDDEWRHCELCGGGSDGKPPILMEHNNNDD 64
           N K    SDD   EK+S    K +E  +  +      C  C      +    M H +   
Sbjct: 305 NGKCSANSDDQLSEKIS----KALEQTSSNI----TICGFCQSARVSEATGEMLHYSRGR 364

Query: 65  EFGDEDGWLGHLVGPIIDHNVIPRKWLHYQCVIWSPVVHFTESVSLKNVQAALKRGKSLK 124
               +D         I   NVI    +H  C+ W+P V++ E  ++KN++A L RG  +K
Sbjct: 365 PVDGDD---------IFRSNVI---HVHSACIEWAPQVYY-EGDTVKNLKAELARGMKIK 424

Query: 125 CTHCKRRGATLGCRLEQCQKTYH------LVRSKFDHKQFLMACTDHGHI 169
           CT C  +GA LGC ++ C+++YH      + R ++D++ FL+ C  H  +
Sbjct: 425 CTKCSLKGAALGCFVKSCRRSYHVPCAREISRCRWDYEDFLLLCPAHSSV 453

BLAST of PI0001227 vs. TAIR 10
Match: AT2G03670.1 (cell division cycle 48B )

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 37/80 (46.25%), Postives = 51/80 (63.75%), Query Frame = 0

Query: 307 NVIDPALRRPGRFDREIYFPLPSFEDRISILSLYTQKWPKTLNSNQPL-LHWIAKKTSGF 366
           + IDPALRR GRFD  +    P+ EDR+ IL LYT+K    +N +  + L  IA   +G+
Sbjct: 175 DAIDPALRRAGRFDALVEVSTPNEEDRLKILQLYTKK----VNLDPSVDLQAIAISCNGY 234

Query: 367 AGADLQALCTQTAINALKRT 386
            GADL+ALC +  I+A KR+
Sbjct: 235 VGADLEALCREATISASKRS 250