PI0016253 (gene) Melon (PI 482460) v1

BLAST of PI0016253 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 4.2e-261
Identity = 468/760 (61.58%), Postives = 575/760 (75.66%), Query Frame = 0

Query: 1   MVFLPFFCICFFLNSHGYVAMKTELSSTSNTNLQT-----YIVHVKQPELGNLGDAIDLQ 60
           M +  F  + F L  H + A  +EL +T+  + +T     YI+HV  PE   L ++ DL+
Sbjct: 1   MDYFLFIALTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLE 60

Query: 61  NWYTSFLPETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILN 120
           +WY SFLP T+  SS EQ R++YSY++V+ GF+A LT+E++ A+E+K+GFISA P+ +L+
Sbjct: 61  SWYHSFLPPTL-MSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLH 120

Query: 121 LHTTRTPEYLGLNQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRC 180
             TT TP++LGL Q  GVWK SNFGKGVIIGVLD+GI P HPSF+D G+  PP KWKGRC
Sbjct: 121 RQTTHTPKFLGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRC 180

Query: 181 EFGASICNNKLIGARTFNLANNVLMGK---SPNDENGHGTHTASTAAGTFVKGAEALGNA 240
           +   + CNNKLIGAR FNLA   + GK   +P DE+GHGTHTASTAAG FV  AE LGNA
Sbjct: 181 DLNVTACNNKLIGARAFNLAAEAMNGKKAEAPIDEDGHGTHTASTAAGAFVNYAEVLGNA 240

Query: 241 RGKAVGMAPLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLG-APSIPFFKDA 300
           +G A GMAP AH+A+YKVC  + C  SDILAALDAA++DGVDV+S+SLG +   PFF D+
Sbjct: 241 KGTAAGMAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDS 300

Query: 301 IAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVY 360
            AIGAFAA++KGIFVSC+AGNSGP  S++ N APWILTVGASTIDR+IVA AKLGNG+ +
Sbjct: 301 TAIGAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEF 360

Query: 361 TGESLFQPRDFPSKFLPLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAK 420
            GES+FQP  F    LPL YAGK+G E S FC  GSL+     GKVV+CERGGGI+RIAK
Sbjct: 361 DGESVFQPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAK 420

Query: 421 GLVVKNGGGAAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISF 480
           G  VK  GGAAMIL+N + + FS  A+ H LP TH+SY  G++IKAYINS+  P A+I F
Sbjct: 421 GEEVKRAGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILF 480

Query: 481 KGTLFGNRAATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTKST 540
           KGT+ GN   + +PA+ASFSSRGP   SPGILKPDI GPGVNILAAWPFPL+N+T++K T
Sbjct: 481 KGTVIGN---SLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLT 540

Query: 541 FNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPAN 600
           FN+ SGTSMSCPHLSGIAAL+KS HP+WSPAAIKSAIMTSAD  N   K IVD+ L+P +
Sbjct: 541 FNIESGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTD 600

Query: 601 FFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVLRIRE 660
            FA GSGHVNPS+A +PGLVYDIQPDDY+PYLC L Y++ +V II  R++ CS    I E
Sbjct: 601 LFATGSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCSA--SIPE 660

Query: 661 GDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEK 720
           G+LNYPSF+V LG+ S+TF RTVTNVG+A+S Y  IV AP G+ V+V P  L FS+VN+K
Sbjct: 661 GELNYPSFSVELGS-SKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQK 720

Query: 721 VTYSVTFSRNDFVRTTSEFSEGYLIWVSNKHMVRSPISVK 751
            TYSVTFSR      T E+++G+L WVS KH VRSPISVK
Sbjct: 721 ETYSVTFSRTGLGNKTQEYAQGFLKWVSTKHTVRSPISVK 753

BLAST of PI0016253 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 1.2e-244
Identity = 447/754 (59.28%), Postives = 551/754 (73.08%), Query Frame = 0

Query: 1   MVFLPFFCICFFLNSHGYVAMKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTS 60
           M  +  F I F L+ H   A +       +T LQTYIVHV +P+   L ++ DL+++Y S
Sbjct: 1   MTCICIFSIAFLLSFHLTTAQR-------STGLQTYIVHVDKPDAQVLANSADLESYYNS 60

Query: 61  FLPETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTR 120
           FLP T+ + S   SR+++SY HV +GF+A+L+ E+VK ME+K GF+SA  E +L LHTT 
Sbjct: 61  FLPATV-SGSEVPSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTH 120

Query: 121 TPEYLGLNQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF-GA 180
           TP +LGL Q+ G W+ SN+GKGVIIG+LDTGI P HPSF+D  M SPPAKWKG+CEF G 
Sbjct: 121 TPNFLGLYQNMGFWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGN 180

Query: 181 SICNNKLIGARTFNLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGM 240
           + CN K+IGAR F   +    G  P DE GHGTHTASTAAG FV  A   GNA G AVGM
Sbjct: 181 ATCNKKIIGARNFISGS----GVPPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGM 240

Query: 241 APLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAA 300
           APLAHIAMYKVCS+ GCS +DILAALDAAIDDGVDVLSLSLG  S PF+ D IAIGAFAA
Sbjct: 241 APLAHIAMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAA 300

Query: 301 IKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQP 360
           I+KGIFVS SAGN GP  STL+NEAPWILTVGAST DRKIVA A LGNG+ Y GES FQP
Sbjct: 301 IRKGIFVSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQP 360

Query: 361 RDFPSKFLPLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGG 420
            DFP   LPLVY G S  E + FC  GSL+K +V GKVVVC+RGG ++R+ K   VK+ G
Sbjct: 361 ADFPHTLLPLVYPGTSD-EEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAG 420

Query: 421 GAAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNR 480
           GAAMIL N + DG  T A+AHVLP TH+ Y  G  IK+YINS+  P A I FKGT+ G +
Sbjct: 421 GAAMILANLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFK 480

Query: 481 AATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTKSTFNVISGTS 540
           +   SP+++SFSSRGP  ASPGI+KPDI GPGVNILAAWP  + N T T  TFN+ISGTS
Sbjct: 481 S---SPSVSSFSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTS 540

Query: 541 MSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGH 600
           MSCPHLSGI AL+KS HP+WSPAAIKSAIMTSAD  N +G+PI+D+   PA+ FA G+GH
Sbjct: 541 MSCPHLSGIVALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGH 600

Query: 601 VNPSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVLRIREGDLNYPSF 660
           VNPSKA++PGL+YDIQ +DY+ YLC L Y +  + +IV+  V C +   I E +LNYPSF
Sbjct: 601 VNPSKASDPGLIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKCES--SISEAELNYPSF 660

Query: 661 AVSLGAGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFS 720
           ++ LG  +Q + RTVTNVGDA+S Y+  +    G+ + V P  L F+K+ ++ TY+V+F+
Sbjct: 661 SIILGPKTQNYTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFT 720

Query: 721 RN-DFVRTTSEFSEGYLIWVSNKHMVRSPISVKL 752
           ++ DF   T  F +G + W SN+++VRSPISVKL
Sbjct: 721 QSGDF---TDRFVQGAISWSSNEYVVRSPISVKL 733

BLAST of PI0016253 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 2.2e-238
Identity = 428/729 (58.71%), Postives = 538/729 (73.80%), Query Frame = 0

Query: 23  TELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLPETIEASSNEQSRLLYSYQH 82
           TEL     +NL TYIVHV++P+   +  + DL  +Y S LPE+ + ++    R++++Y++
Sbjct: 32  TELEDEDQSNLSTYIVHVRKPQ---VIQSDDLHTFYYSLLPESTKTTN---QRIVFTYRN 91

Query: 83  VMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLNQHFGVWKNSNFGKG 142
           V++GF+ +LT E+ KA+++ +  +SA PE IL+LHTT TP +LGL Q  G+WK SN GKG
Sbjct: 92  VVNGFAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKG 151

Query: 143 VIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF-GASICNNKLIGARTFNLANNVLMG 202
           VIIG+LDTGI P HPSF+DEGM SPPAKW G CEF G   CNNK+IGAR F    N+ + 
Sbjct: 152 VIIGILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLTL- 211

Query: 203 KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSQKGCSSSDI 262
             P D+ GHGTHTASTAAG  V+GA   GNA G AVGMAP AHIAMYKVC   GCS S I
Sbjct: 212 --PFDDVGHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAI 271

Query: 263 LAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFVSCSAGNSGPSKSTLA 322
           LA +D A+DDGVDVLSLSLG PS PFF+D IA+GAF AI+KGIFVSCSA NSGP+ S+L+
Sbjct: 272 LAGMDTAVDDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLS 331

Query: 323 NEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFLPLVYAGKSGIEG-S 382
           NEAPWILTVGAS+IDR I+A AKLGNGK Y G+S+FQP+DF    LPLVYAG +G    S
Sbjct: 332 NEAPWILTVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFS 391

Query: 383 EFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILINQKPDGFSTLAEAH 442
            FC   SL + +V GKVV+CE GG + R+ KG  VK+ GGAAMIL+N   + F+ +A+ H
Sbjct: 392 VFCAPESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVH 451

Query: 443 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAMASFSSRGPCQASP 502
           VLP  H+SYE GL +K YINS+  P A+I F+GT+ GN  A   P + SFSSRGP +ASP
Sbjct: 452 VLPAVHISYEAGLALKEYINSTSTPTATILFEGTVIGNLLA---PQVTSFSSRGPSKASP 511

Query: 503 GILKPDITGPGVNILAAWPFPLNNNTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWS 562
           GILKPDI GPG+NILAAWP  L+N+T     FN+ISGTSMSCPHLSGIAAL+K+ HP+WS
Sbjct: 512 GILKPDIIGPGLNILAAWPVSLDNSTT--PPFNIISGTSMSCPHLSGIAALLKNSHPDWS 571

Query: 563 PAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYV 622
           PAAIKSAIMT+A   N  G PI+DQ L PA+ FA G+GHVNP KA +PGLVYDI+P+DY+
Sbjct: 572 PAAIKSAIMTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYI 631

Query: 623 PYLCHL-YTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDA 682
           PYLC L YTD +V +I++++V CS V  I E +LNYPSF++ LG  +Q + RTV NVG A
Sbjct: 632 PYLCGLNYTDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPA 691

Query: 683 NSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFSRNDFVRTTSEFSEGYLIWVSN 742
           NS Y+A +  PVG+ + ++P  L F++V +K+TYSV+F      R    F++G L WVS 
Sbjct: 692 NSTYTAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSG 746

Query: 743 KHMVRSPIS 749
           K+ VRSPIS
Sbjct: 752 KYSVRSPIS 746

BLAST of PI0016253 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 6.7e-235
Identity = 429/725 (59.17%), Postives = 535/725 (73.79%), Query Frame = 0

Query: 32  NLQTYIVHVKQPEL-GNLGDAIDLQNWYTSFLPETIEASSNEQSRLLYSYQHVMSGFSAR 91
           NL TYIVHVK+ E+ G L    +L  W+ SFLPET     + + R+++SY++V SGF+ R
Sbjct: 37  NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPET-----SNKDRMVFSYRNVASGFAVR 96

Query: 92  LTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLNQHFGVWKNSNFGKGVIIGVLDT 151
           LT E+  A++EK+  +S  PE  L+LHTT TP +LGL Q  G+W +SN GKGVIIGV+DT
Sbjct: 97  LTPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDT 156

Query: 152 GIHPNHPSFNDEGMSSPPAKWKGRCEF-GASICNNKLIGARTFNLANNVLMGKSPNDENG 211
           GI+P H SFNDEGM  PPAKWKG CEF G S+CNNKLIGAR  NL  + +  + P ++  
Sbjct: 157 GIYPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-EPPYEDFF 216

Query: 212 HGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSQK---GCSSSDILAALD 271
           HGTHTA+ AAG FV+GA   GNARG A GMAP AH+A+YKVCS K    C  S ILAA+D
Sbjct: 217 HGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMD 276

Query: 272 AAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEAPW 331
            AI+DGVDVLSLSLG  S+PFF+D IAIGAFAA +KGIFVSCSA NSGP  S+L+NEAPW
Sbjct: 277 IAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPW 336

Query: 332 ILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFLPLVYAGKSGIEGSEFCVQG 391
           ILTVGASTIDRKI A AKLGNG  Y GE+LFQP+DF S+ LPLVYA       S  C  G
Sbjct: 337 ILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPG 396

Query: 392 SLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILINQKPDGFSTLAEAHVLPTTH 451
           SL  +NV GKVVVC+ GGGI  IAKG  V + GG+AMIL N +  GF+TLA AHVLP  H
Sbjct: 397 SLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVH 456

Query: 452 LSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAMASFSSRGPCQASPGILKPD 511
           +SY   L IKAYINS++ P A++ F+GT+ G+   + +P++A+FSSRGP Q SPGILKPD
Sbjct: 457 VSYAASLAIKAYINSTYTPTATVLFQGTIIGD---SLAPSVAAFSSRGPSQQSPGILKPD 516

Query: 512 ITGPGVNILAAWPFPLNNNTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKS 571
           I GPGVNILAAW   ++N       F++ISGTSMSCPHLSGIAAL+KS HP+WSPAAIKS
Sbjct: 517 IIGPGVNILAAWAVSVDNKI---PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKS 576

Query: 572 AIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHL 631
           AIMT+A+  N +G PI+DQ L+PA+ FA G+GHVNP +A +PGLVYDIQP+DYVPYLC L
Sbjct: 577 AIMTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGL 636

Query: 632 -YTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYSA 691
            Y+D +V+IIV+R V C  V  I + +LNYPSF++ LG+ SQ + RT+TNVG ANS Y+ 
Sbjct: 637 GYSDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTV 696

Query: 692 IVKAPVGISVRVTPRNLKFSKVNEKVTYSVTF-SRNDFVRTTSEFSEGYLIWVSNKHMVR 750
            +  P+ + + V+P  + F++VN+KV Y V F  +    R    F++G + WVS+KH+VR
Sbjct: 697 KIDVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVR 747

BLAST of PI0016253 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 1.8e-227
Identity = 421/749 (56.21%), Postives = 531/749 (70.89%), Query Frame = 0

Query: 9   ICFFLNSHGYVAMKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLPETIEA 68
           + F L S   +A +  L      NL TYIVHVK+ E      + DL +WY SFLP+T   
Sbjct: 12  LIFILCSISMLAAEENLEH-DQINLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFP- 71

Query: 69  SSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLN 128
               + R+++SY+ V SGF+ +LT E+ K+++EK   +SA PE  L LHTT TP +LGL 
Sbjct: 72  ---HKERMVFSYRKVASGFAVKLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLK 131

Query: 129 QHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF-GASICNNKLI 188
           Q  G+W + N GKGVIIG++DTGI P HPSFNDEGM  PPAKWKG CEF G  +CNNKLI
Sbjct: 132 QGQGLWSDDNLGKGVIIGIIDTGIFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLI 191

Query: 189 GARTFNLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAM 248
           GAR  NL  + +  + P +   HGTHTA+ AAG F++ A   GNA+G A GMAP AH+A+
Sbjct: 192 GAR--NLVKSAIQ-EPPFENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAI 251

Query: 249 YKVCSQK-GCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFV 308
           YKVC+ K GC+ S ILAA+D AI+DGVDVLSLSLG  S+PFF+D IAIGAFAA + G+FV
Sbjct: 252 YKVCNDKIGCTESAILAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFV 311

Query: 309 SCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKF 368
           SCSA NSGP  STL+NEAPWILTVGASTIDRKIVA AKLGNG+ Y GE+LFQP+DF  + 
Sbjct: 312 SCSAANSGPGYSTLSNEAPWILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQL 371

Query: 369 LPLVYAGKSG----IEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAA 428
           LPLVY G  G     +    C+ GSL+ ++++GKVV+C+  G +S I KG  V N GG A
Sbjct: 372 LPLVYPGSFGYGNQTQNQSLCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIA 431

Query: 429 MILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAAT 488
           MIL N +  GFST A AHVLP   +SY  GL IK+YI S++NP A++ FKGT+ G+   +
Sbjct: 432 MILANSEALGFSTFAIAHVLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGD---S 491

Query: 489 FSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTKSTFNVISGTSMSC 548
            +P++  FSSRGP Q SPGILKPDI GPGVNILAAW   ++N       F+++SGTSMSC
Sbjct: 492 LAPSVVYFSSRGPSQESPGILKPDIIGPGVNILAAWAVSVDNKI---PAFDIVSGTSMSC 551

Query: 549 PHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNP 608
           PHLSGIAALIKS HP+WSPAAIKSAIMT+A+  N  G PI+DQ L PA+ FA G+GHVNP
Sbjct: 552 PHLSGIAALIKSSHPDWSPAAIKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNP 611

Query: 609 SKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVS 668
            KA +PGLVYDI+P+DYVPYLC L Y+D ++ +IV+ +V CS V  I E  LNYPSF++ 
Sbjct: 612 VKANDPGLVYDIEPEDYVPYLCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSIL 671

Query: 669 LGAGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTF-SRN 728
           LG+ SQ + RT+TNVG ANS Y   ++ P+ + + V P  + F++VNEKV++SV F  + 
Sbjct: 672 LGSDSQYYTRTLTNVGFANSTYKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQI 731

Query: 729 DFVRTTSEFSEGYLIWVSNKHMVRSPISV 750
              R    F +G L WVS++H VR PISV
Sbjct: 732 KENRRNHTFGQGSLTWVSDRHAVRIPISV 745

BLAST of PI0016253 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 1399.4 bits (3621), Expect = 0.0e+00
Identity = 702/754 (93.10%), Postives = 725/754 (96.15%), Query Frame = 0

Query: 1   MVFLPFFCICFFLNSHGYVAMKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTS 60
           MVFLPF CI FFLN HGYVAMKTELSSTSNTNLQTYIVHVKQP L  LGD IDLQNWYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60

Query: 61  FLPETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTR 120
           FLPETIEASS+EQ RLLYSY+HVMSGFSARLTKEQVKAMEEKDGFISA PET LNLHTT 
Sbjct: 61  FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH 120

Query: 121 TPEYLGLNQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180
           TPEYLGLN+HFG+WKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS
Sbjct: 121 TPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180

Query: 181 ICNNKLIGARTFNLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240
           ICNNKLIGARTFNLANN ++GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA
Sbjct: 181 ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240

Query: 241 PLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAI 300
           PLAHIAMYKVCS  GCSSSDILAALDAAIDDGVDVLSLSLGAPS PFF+D IA+GAFAAI
Sbjct: 241 PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAI 300

Query: 301 KKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPR 360
           KKGIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDRKIVALAKLG+G+VYTGESLFQPR
Sbjct: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR 360

Query: 361 DFPSKFLPLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGG 420
           +FPSKFLPLVYAGKSGIEGSE+C+QGSLEKLNVTGKVVVCERGGGISR+AKGLVVKNGGG
Sbjct: 361 NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG 420

Query: 421 AAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRA 480
           AAMILINQKP+GFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTL GNRA
Sbjct: 421 AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA 480

Query: 481 ATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNN--NTNTKSTFNVISGT 540
            TFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNN  NTNTKSTFNVISGT
Sbjct: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540

Query: 541 SMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSG 600
           SMSCPHLSGIAALIKS HPNWSPAAIKSAIMTSADVRNPQGKPI+DQDLKPANFFAMGSG
Sbjct: 541 SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG 600

Query: 601 HVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPSF 660
           HVNPSKAANPGLVYDIQPDDY+PYLCHLYTDAQVSIIVR+QVTCSTV RIREGDLNYPSF
Sbjct: 601 HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF 660

Query: 661 AVSLGAGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFS 720
           AVSLGAGSQTFNRTVTNVGDANSVY  IV+AP G+SV+VTP NLKFSK+NEKVTYSVTFS
Sbjct: 661 AVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS 720

Query: 721 RNDFVRTTSEFSEGYLIWVSNKHMVRSPISVKLT 753
           R DFVRTTSEFSEGYLIWVS K MVRSPISVKLT
Sbjct: 721 RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT 754

BLAST of PI0016253 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 1364.7 bits (3531), Expect = 0.0e+00
Identity = 685/734 (93.32%), Postives = 704/734 (95.91%), Query Frame = 0

Query: 21  MKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLPETIEASSNEQSRLLYSY 80
           MKTEL S SNTNLQTYIVHVKQPE+  LGD IDLQNWYTSFLPETIEASSNEQSRLLYSY
Sbjct: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60

Query: 81  QHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLNQHFGVWKNSNFG 140
           +HV+SGFSARLTKEQVK MEEKDGFISA PET LNLHTT TPEYLGLNQHFG+WKNSNFG
Sbjct: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120

Query: 141 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNVLM 200
           KGVIIGVLDTGIHPNHPSFNDEGM SPPAKWKGRCEFGASICNNKLIGARTFNLANNV +
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180

Query: 201 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSQKGCSSSD 260
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIA+YKVCS KGCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240

Query: 261 ILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFVSCSAGNSGPSKSTL 320
           ILAALDAAIDDGVDVLSLSLGAPS PFFKD IA+GAFAAIKKGIFVSCSAGNSGPSK+TL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 321 ANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFLPLVYAGKSGIEGS 380
           ANEAPWILTVGASTIDRKIVALAKL +GKV+TGESLFQPRDF SKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360

Query: 381 EFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILINQKPDGFSTLAEAH 440
           E+CV+GSLEKLNVTGK+VVCERGGGI RIAKGLVVKNGGGAAMIL+NQKPDGFSTLAEAH
Sbjct: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420

Query: 441 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAMASFSSRGPCQASP 500
           VLPTTHLSYEDGLKIK YINSSHNPKASISF+GTL GNRA TFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 501 GILKPDITGPGVNILAAWPFPLNN--NTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPN 560
           GILKPDITGPGVNILAAWPFPLNN  NTNTKSTFNVISGTSMSCPHLSGIAALIKS HPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540

Query: 561 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 620
           WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 621 YVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGD 680
           YVPYLCHLYTDAQVSIIVRRQVTCSTV RIREGDLNYPSFAVSLGA SQ FNRTVTNVGD
Sbjct: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660

Query: 681 ANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFSRNDFVRTTSEFSEGYLIWVS 740
           ANSVY AIVKAP G+SVRVTPRNLKFSK+NEK+TYSVTFSR DFVRT SEFSEGYLIWVS
Sbjct: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720

Query: 741 NKHMVRSPISVKLT 753
           NKH+VRSPISVKLT
Sbjct: 721 NKHIVRSPISVKLT 734

BLAST of PI0016253 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 1364.4 bits (3530), Expect = 0.0e+00
Identity = 685/734 (93.32%), Postives = 706/734 (96.19%), Query Frame = 0

Query: 21  MKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLPETIEASSNEQSRLLYSY 80
           MKTELSSTSNTNLQTYIVHVKQP L  LGD IDLQNWYTSFLPETIEASS+EQ RLLYSY
Sbjct: 1   MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 60

Query: 81  QHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLNQHFGVWKNSNFG 140
           +HVMSGFSARLTKEQVKAMEEKDGFISA PET LNLHTT TPEYLGLN HFG+WKNSNFG
Sbjct: 61  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG 120

Query: 141 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNVLM 200
           KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANN ++
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 180

Query: 201 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSQKGCSSSD 260
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCS  GCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 240

Query: 261 ILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFVSCSAGNSGPSKSTL 320
           ILAALDAAIDDGVDVLSLSLGAPS PFF+D IAIGAFAAIKKGIFVSCSAGNSGPSK+TL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 321 ANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFLPLVYAGKSGIEGS 380
           ANEAPWILTVGASTIDRKIVALAKLG+G+VYTGESLFQPR+FPSKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 360

Query: 381 EFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILINQKPDGFSTLAEAH 440
           E+C+QGSLEKLNVTGKVVVCERGGGISR+AKGLVVKNGGGAAMILINQKP+GFSTLAEAH
Sbjct: 361 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 420

Query: 441 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAMASFSSRGPCQASP 500
           VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTL GNRA TFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 501 GILKPDITGPGVNILAAWPFPLNN--NTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPN 560
           GILKPDITGPGVNILAAWPFPLNN  NTNTKSTFNVISGTSMSCPHLSGIAALIKS HPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 540

Query: 561 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 620
           WSPAAIKSAIMTSADVRNPQGKPI+DQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 621 YVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGD 680
           Y+PYLCHLYTDAQVSIIVR+QVTCSTV RIREGDLNYPSFAVSL AGSQTFNRTVTNVGD
Sbjct: 601 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD 660

Query: 681 ANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFSRNDFVRTTSEFSEGYLIWVS 740
           ANSVY  IV+AP G+SV+VTP NLKFSK+NEKVTYSVTFSR DFVRTTSEFSEGYLIWVS
Sbjct: 661 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 720

Query: 741 NKHMVRSPISVKLT 753
            K MVRSPISVKLT
Sbjct: 721 KKLMVRSPISVKLT 734

BLAST of PI0016253 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 547/755 (72.45%), Postives = 643/755 (85.17%), Query Frame = 0

Query: 1   MVFLPFFCICFFLNSHGYVAMKTELSSTS-NTNLQTYIVHVKQPELGNLGDAID--LQNW 60
           MV L F  I F LN   +VA K EL +T   + LQTYIVHV+QP++  +G++ +  +++W
Sbjct: 1   MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60

Query: 61  YTSFLPETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLH 120
           Y SF+P++ E ++ EQ +LLYSY++VMSGFSARLT EQVKAME+KDGFISA PETI++LH
Sbjct: 61  YMSFIPKSTE-TTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLH 120

Query: 121 TTRTPEYLGLNQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF 180
           TT TPEYLGLNQ FG+WKNSNFGKGVI+GVLDTGIHPNHPSFNDEGMS PPAKWKG+CEF
Sbjct: 121 TTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEF 180

Query: 181 GASICNNKLIGARTFNLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAV 240
            +S+CNNKLIGARTFNL NN LM +SPNDE GHGTHTASTAAG FV+ AEALGNA+GKA 
Sbjct: 181 NSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAA 240

Query: 241 GMAPLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAF 300
           G+APLAH+A+YKVCS K C SSD+ A +DAAIDDGVDVLS+SLG+ S+PFFKD IA+  F
Sbjct: 241 GIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATF 300

Query: 301 AAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLF 360
            AI+KGIFVS SAGNSGP  STL+N+APWILTVGASTI+R+IVA+AKLGNG+ Y GESL+
Sbjct: 301 GAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLY 360

Query: 361 QPRDFPSKFLPLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKN 420
           QP DFPSKFLPLVYAG    +   FC +GSLE ++V GKVVVCE  GG+ R+AKGLVVKN
Sbjct: 361 QPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKN 420

Query: 421 GGGAAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFG 480
            GGAAMILINQ+ DGFSTL+EAHVLP TH+SY+ G+ IK+YINSS NP ASISFKGT+ G
Sbjct: 421 AGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIG 480

Query: 481 NRAATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTKSTFNVISG 540
           +     +P+MASFSSRGPC  SPGILKPDITGPGVNILAAWPFPL+N+TNTKSTFNVISG
Sbjct: 481 DGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISG 540

Query: 541 TSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGS 600
           TSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT+A+++ PQG+PIVDQDL+PANFFAMG+
Sbjct: 541 TSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAMGA 600

Query: 601 GHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPS 660
           GHVNPSKAA+PGLVYDIQPDDY+PYLC LY D +VSIIV R V C  V  IREGDLNYPS
Sbjct: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNYPS 660

Query: 661 FAVSLGAGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTF 720
           F+V+LG G QTF RTVTNVG+ANSVY+AIV+AP+G+S+ V PR L FS+VN+ +T++VTF
Sbjct: 661 FSVALG-GLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTF 720

Query: 721 SRNDFVRTTSEFSEGYLIWV--SNKHMVRSPISVK 751
           +R  +V+   EF EGYL WV  S K++VRSP+SVK
Sbjct: 721 NRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVK 753

BLAST of PI0016253 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 542/745 (72.75%), Postives = 638/745 (85.64%), Query Frame = 0

Query: 11  FFLNSHGYVAMKTELSSTS-NTNLQTYIVHVKQPELGNLGDAID--LQNWYTSFLPETIE 70
           F LN   +VA K EL +T   + LQTYIVHV+QP++  +G++ +  +++WY SF+P++ E
Sbjct: 20  FLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTE 79

Query: 71  ASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGL 130
            ++ EQ +LLYSY++VMSGFSARLT EQVKAME+KDGFISA PETI++LHTT TPEYLGL
Sbjct: 80  -TTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGL 139

Query: 131 NQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLI 190
           NQ FG+WKNSNFGKGVI+GVLDTGIHPNHPSFNDEGMS PPAKWKG+CEF +S+CNNKLI
Sbjct: 140 NQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLI 199

Query: 191 GARTFNLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAM 250
           GARTFNL NN LM +SPNDE GHGTHTASTAAG FV+ AEALGNA+GKA G+APLAH+A+
Sbjct: 200 GARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAI 259

Query: 251 YKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFVS 310
           YKVCS K C SSD+ A +DAAIDDGVDVLS+SLG+ S+PFFKD IA+  F AI+KGIFVS
Sbjct: 260 YKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVS 319

Query: 311 CSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFL 370
            SAGNSGP  STL+N+APWILTVGASTI+R+IVA+AKLGNG+ Y GESL+QP DFPSKFL
Sbjct: 320 SSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFL 379

Query: 371 PLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILIN 430
           PLVYAG    +   FC +GSLE ++V GKVVVCE  GG+ R+AKGLVVKN GGAAMILIN
Sbjct: 380 PLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILIN 439

Query: 431 QKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAM 490
           Q+ DGFSTL+EAHVLP TH+SY+ G+ IK+YINSS NP ASISFKGT+ G+     +P+M
Sbjct: 440 QEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSM 499

Query: 491 ASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTKSTFNVISGTSMSCPHLSG 550
           ASFSSRGPC  SPGILKPDITGPGVNILAAWPFPL+N+TNTKSTFNVISGTSMSCPHLSG
Sbjct: 500 ASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSG 559

Query: 551 IAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAAN 610
           IAALIKSVHPNWSPAAIKSAIMT+A+++ PQG+PI DQDL+PANFFAMG+GHVNPSKAA+
Sbjct: 560 IAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAAD 619

Query: 611 PGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVSLGAGSQ 670
           PGLVYDIQPDDY+PYLC LY D +VSIIV R V C  VL IREGDLNYPSF+V+LG G Q
Sbjct: 620 PGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVLSIREGDLNYPSFSVALG-GLQ 679

Query: 671 TFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFSRNDFVRTTS 730
           TF RTVTNVG+ANSVY+AIV+AP+G+S+ V PR L FS+VN+ +T++VTF+R  +V+   
Sbjct: 680 TFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVG 739

Query: 731 EFSEGYLIWV--SNKHMVRSPISVK 751
           EF EGYL WV  S K++VRSP+SVK
Sbjct: 740 EFGEGYLKWVSKSKKYVVRSPVSVK 762

BLAST of PI0016253 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 1399.4 bits (3621), Expect = 0.0e+00
Identity = 702/754 (93.10%), Postives = 725/754 (96.15%), Query Frame = 0

Query: 1   MVFLPFFCICFFLNSHGYVAMKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTS 60
           MVFLPF CI FFLN HGYVAMKTELSSTSNTNLQTYIVHVKQP L  LGD IDLQNWYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60

Query: 61  FLPETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTR 120
           FLPETIEASS+EQ RLLYSY+HVMSGFSARLTKEQVKAMEEKDGFISA PET LNLHTT 
Sbjct: 61  FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH 120

Query: 121 TPEYLGLNQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180
           TPEYLGLN+HFG+WKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS
Sbjct: 121 TPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180

Query: 181 ICNNKLIGARTFNLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240
           ICNNKLIGARTFNLANN ++GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA
Sbjct: 181 ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240

Query: 241 PLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAI 300
           PLAHIAMYKVCS  GCSSSDILAALDAAIDDGVDVLSLSLGAPS PFF+D IA+GAFAAI
Sbjct: 241 PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAI 300

Query: 301 KKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPR 360
           KKGIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDRKIVALAKLG+G+VYTGESLFQPR
Sbjct: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR 360

Query: 361 DFPSKFLPLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGG 420
           +FPSKFLPLVYAGKSGIEGSE+C+QGSLEKLNVTGKVVVCERGGGISR+AKGLVVKNGGG
Sbjct: 361 NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG 420

Query: 421 AAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRA 480
           AAMILINQKP+GFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTL GNRA
Sbjct: 421 AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA 480

Query: 481 ATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNN--NTNTKSTFNVISGT 540
            TFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNN  NTNTKSTFNVISGT
Sbjct: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540

Query: 541 SMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSG 600
           SMSCPHLSGIAALIKS HPNWSPAAIKSAIMTSADVRNPQGKPI+DQDLKPANFFAMGSG
Sbjct: 541 SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG 600

Query: 601 HVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPSF 660
           HVNPSKAANPGLVYDIQPDDY+PYLCHLYTDAQVSIIVR+QVTCSTV RIREGDLNYPSF
Sbjct: 601 HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF 660

Query: 661 AVSLGAGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFS 720
           AVSLGAGSQTFNRTVTNVGDANSVY  IV+AP G+SV+VTP NLKFSK+NEKVTYSVTFS
Sbjct: 661 AVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS 720

Query: 721 RNDFVRTTSEFSEGYLIWVSNKHMVRSPISVKLT 753
           R DFVRTTSEFSEGYLIWVS K MVRSPISVKLT
Sbjct: 721 RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT 754

BLAST of PI0016253 vs. NCBI nr
Match: XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])

HSP 1 Score: 1397.1 bits (3615), Expect = 0.0e+00
Identity = 702/754 (93.10%), Postives = 721/754 (95.62%), Query Frame = 0

Query: 1   MVFLPFFCICFFLNSHGYVAMKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTS 60
           MVFLPF CI FFLN HGYVAMKTEL S SNTNLQTYIVHVKQPE+  LGD IDLQNWYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTS 60

Query: 61  FLPETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTR 120
           FLPETIEASSNEQSRLLYSY+HV+SGFSARLTKEQVK MEEKDGFISA PET LNLHTT 
Sbjct: 61  FLPETIEASSNEQSRLLYSYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTH 120

Query: 121 TPEYLGLNQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180
           TPEYLGLNQHFG+WKNSNFGKGVIIGVLDTGIHPNHPSFNDEGM SPPAKWKGRCEFGAS
Sbjct: 121 TPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGAS 180

Query: 181 ICNNKLIGARTFNLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240
           ICNNKLIGARTFNLANNV +GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA
Sbjct: 181 ICNNKLIGARTFNLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240

Query: 241 PLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAI 300
           PLAHIA+YKVCS KGCSSSDILAALDAAIDDGVDVLSLSLGAPS PFFKD IA+GAFAAI
Sbjct: 241 PLAHIAVYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAI 300

Query: 301 KKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPR 360
           KKGIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDRKIVALAKL +GKV+TGESLFQPR
Sbjct: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPR 360

Query: 361 DFPSKFLPLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGG 420
           DF SKFLPLVYAGKSGIEGSE+CV+GSLEKLNVTGK+VVCERGGGI RIAKGLVVKNGGG
Sbjct: 361 DFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGG 420

Query: 421 AAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRA 480
           AAMIL+NQKPDGFSTLAEAHVLPTTHLSYEDGLKIK YINSSHNPKASISF+GTL GNRA
Sbjct: 421 AAMILVNQKPDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRA 480

Query: 481 ATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNN--NTNTKSTFNVISGT 540
            TFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNN  NTNTKSTFNVISGT
Sbjct: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540

Query: 541 SMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSG 600
           SMSCPHLSGIAALIKS HPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSG
Sbjct: 541 SMSCPHLSGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSG 600

Query: 601 HVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPSF 660
           HVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTV RIREGDLNYPSF
Sbjct: 601 HVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSF 660

Query: 661 AVSLGAGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFS 720
           AVSLGA SQ FNRTVTNVGDANSVY AIVKAP G+SVRVTPRNLKFSK+NEK+TYSVTFS
Sbjct: 661 AVSLGADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFS 720

Query: 721 RNDFVRTTSEFSEGYLIWVSNKHMVRSPISVKLT 753
           R DFVRT SEFSEGYLIWVSNKH+VRSPISVKLT
Sbjct: 721 RIDFVRTRSEFSEGYLIWVSNKHIVRSPISVKLT 754

BLAST of PI0016253 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1364.4 bits (3530), Expect = 0.0e+00
Identity = 685/734 (93.32%), Postives = 706/734 (96.19%), Query Frame = 0

Query: 21  MKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLPETIEASSNEQSRLLYSY 80
           MKTELSSTSNTNLQTYIVHVKQP L  LGD IDLQNWYTSFLPETIEASS+EQ RLLYSY
Sbjct: 1   MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 60

Query: 81  QHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLNQHFGVWKNSNFG 140
           +HVMSGFSARLTKEQVKAMEEKDGFISA PET LNLHTT TPEYLGLN HFG+WKNSNFG
Sbjct: 61  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG 120

Query: 141 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNVLM 200
           KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANN ++
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 180

Query: 201 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSQKGCSSSD 260
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCS  GCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 240

Query: 261 ILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFVSCSAGNSGPSKSTL 320
           ILAALDAAIDDGVDVLSLSLGAPS PFF+D IAIGAFAAIKKGIFVSCSAGNSGPSK+TL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 321 ANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFLPLVYAGKSGIEGS 380
           ANEAPWILTVGASTIDRKIVALAKLG+G+VYTGESLFQPR+FPSKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 360

Query: 381 EFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILINQKPDGFSTLAEAH 440
           E+C+QGSLEKLNVTGKVVVCERGGGISR+AKGLVVKNGGGAAMILINQKP+GFSTLAEAH
Sbjct: 361 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 420

Query: 441 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAMASFSSRGPCQASP 500
           VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTL GNRA TFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 501 GILKPDITGPGVNILAAWPFPLNN--NTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPN 560
           GILKPDITGPGVNILAAWPFPLNN  NTNTKSTFNVISGTSMSCPHLSGIAALIKS HPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 540

Query: 561 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 620
           WSPAAIKSAIMTSADVRNPQGKPI+DQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 621 YVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGD 680
           Y+PYLCHLYTDAQVSIIVR+QVTCSTV RIREGDLNYPSFAVSL AGSQTFNRTVTNVGD
Sbjct: 601 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD 660

Query: 681 ANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFSRNDFVRTTSEFSEGYLIWVS 740
           ANSVY  IV+AP G+SV+VTP NLKFSK+NEKVTYSVTFSR DFVRTTSEFSEGYLIWVS
Sbjct: 661 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 720

Query: 741 NKHMVRSPISVKLT 753
            K MVRSPISVKLT
Sbjct: 721 KKLMVRSPISVKLT 734

BLAST of PI0016253 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 1239.9 bits (3207), Expect = 0.0e+00
Identity = 628/732 (85.79%), Postives = 677/732 (92.49%), Query Frame = 0

Query: 21  MKTELSSTSN-TNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLPETIEASSNEQSRLLYS 80
           MK+ELS TSN   LQTYIVHVK+ EL  LGDAIDL++WYTSFLPETI+ +SNEQSRLLYS
Sbjct: 1   MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIK-TSNEQSRLLYS 60

Query: 81  YQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLNQHFGVWKNSNF 140
           +++VMSGFSARLT E+VKAMEEK+GFISA PETILNLHTT TPEYLGLNQ FG+WK+SNF
Sbjct: 61  FRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 120

Query: 141 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNVL 200
           GKGVIIGVLDTGI P+HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFN AN+ L
Sbjct: 121 GKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSAL 180

Query: 201 MGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSQKGCSSS 260
           MG+SP DENGHGTHTASTAAGTFV+GAEALGNARGKAVGMAPLAH+AMYKVCS KGCSSS
Sbjct: 181 MGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSS 240

Query: 261 DILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFVSCSAGNSGPSKST 320
           DILAALDAAI DGVDVLSLSLGAPS PF+ D IAIGAFAAIK GIFVSCSAGNSGPSKST
Sbjct: 241 DILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKST 300

Query: 321 LANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFLPLVYAGKSGIEG 380
           LANEAPWILTVGASTIDR I+ALA+LGNG+V+ GESLFQPRDFPS FLPLVYAG SG++G
Sbjct: 301 LANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKG 360

Query: 381 SEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILINQKPDGFSTLAEA 440
           SEFCVQGSL  LNV GK+VVCERGGGI RIAKG+VVKN GGAAMI+INQK D FSTLAEA
Sbjct: 361 SEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEA 420

Query: 441 HVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAMASFSSRGPCQAS 500
           H+LP TH+SYE GLKIK+YI SS NP ASISF+GTL G+RA TFSPAMASFSSRGPC  S
Sbjct: 421 HILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPS 480

Query: 501 PGILKPDITGPGVNILAAWPFPLNNNTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNW 560
           PGILKPDITGPGVNILAAWPF L+NNTN KSTFNVISGTSMSCPHLSGIAALIKSVHPNW
Sbjct: 481 PGILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNW 540

Query: 561 SPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDY 620
           SPAAIKSAIMTSADVRN QG+PIVDQDLKPANFFAMGSGHVNPSKAA+PGLVYDIQPDDY
Sbjct: 541 SPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDY 600

Query: 621 VPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDA 680
           +PYLC LY+DAQVSIIVRR+VTCST+ RIREGDLNYPSFAV+LG  SQ FNRTVTNVGDA
Sbjct: 601 IPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGP-SQMFNRTVTNVGDA 660

Query: 681 NSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFSRNDFVRTTSEFSEGYLIWVSN 740
           NS+YSAIV+AP+G+SV+VTP NLKFS+V EKVTYSVTFSR+D VRTTSEFSEGYLIWVSN
Sbjct: 661 NSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSN 720

Query: 741 KHMVRSPISVKL 752
           KHMVRSPISVKL
Sbjct: 721 KHMVRSPISVKL 729

BLAST of PI0016253 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 547/755 (72.45%), Postives = 643/755 (85.17%), Query Frame = 0

Query: 1   MVFLPFFCICFFLNSHGYVAMKTELSSTS-NTNLQTYIVHVKQPELGNLGDAID--LQNW 60
           MV L F  I F LN   +VA K EL +T   + LQTYIVHV+QP++  +G++ +  +++W
Sbjct: 1   MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60

Query: 61  YTSFLPETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLH 120
           Y SF+P++ E ++ EQ +LLYSY++VMSGFSARLT EQVKAME+KDGFISA PETI++LH
Sbjct: 61  YMSFIPKSTE-TTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLH 120

Query: 121 TTRTPEYLGLNQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF 180
           TT TPEYLGLNQ FG+WKNSNFGKGVI+GVLDTGIHPNHPSFNDEGMS PPAKWKG+CEF
Sbjct: 121 TTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEF 180

Query: 181 GASICNNKLIGARTFNLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAV 240
            +S+CNNKLIGARTFNL NN LM +SPNDE GHGTHTASTAAG FV+ AEALGNA+GKA 
Sbjct: 181 NSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAA 240

Query: 241 GMAPLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAF 300
           G+APLAH+A+YKVCS K C SSD+ A +DAAIDDGVDVLS+SLG+ S+PFFKD IA+  F
Sbjct: 241 GIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATF 300

Query: 301 AAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLF 360
            AI+KGIFVS SAGNSGP  STL+N+APWILTVGASTI+R+IVA+AKLGNG+ Y GESL+
Sbjct: 301 GAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLY 360

Query: 361 QPRDFPSKFLPLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKN 420
           QP DFPSKFLPLVYAG    +   FC +GSLE ++V GKVVVCE  GG+ R+AKGLVVKN
Sbjct: 361 QPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKN 420

Query: 421 GGGAAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFG 480
            GGAAMILINQ+ DGFSTL+EAHVLP TH+SY+ G+ IK+YINSS NP ASISFKGT+ G
Sbjct: 421 AGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIG 480

Query: 481 NRAATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTKSTFNVISG 540
           +     +P+MASFSSRGPC  SPGILKPDITGPGVNILAAWPFPL+N+TNTKSTFNVISG
Sbjct: 481 DGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISG 540

Query: 541 TSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGS 600
           TSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT+A+++ PQG+PIVDQDL+PANFFAMG+
Sbjct: 541 TSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAMGA 600

Query: 601 GHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVLRIREGDLNYPS 660
           GHVNPSKAA+PGLVYDIQPDDY+PYLC LY D +VSIIV R V C  V  IREGDLNYPS
Sbjct: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNYPS 660

Query: 661 FAVSLGAGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTF 720
           F+V+LG G QTF RTVTNVG+ANSVY+AIV+AP+G+S+ V PR L FS+VN+ +T++VTF
Sbjct: 661 FSVALG-GLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTF 720

Query: 721 SRNDFVRTTSEFSEGYLIWV--SNKHMVRSPISVK 751
           +R  +V+   EF EGYL WV  S K++VRSP+SVK
Sbjct: 721 NRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVK 753

BLAST of PI0016253 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 621.7 bits (1602), Expect = 7.7e-178
Identity = 352/749 (47.00%), Postives = 478/749 (63.82%), Query Frame = 0

Query: 27  STSNTNLQTYIVHVKQPELGNLGDAIDL-QNWYTSFLPETIEASSNEQSRLLYSYQHVMS 86
           S+S+++  TYIVH+ + ++ +   + DL  NWY S L      S ++ + LLY+Y++ + 
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPS---SFDLHSNWYDSSL-----RSISDSAELLYTYENAIH 82

Query: 87  GFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLNQHFG-VWKNSNFGKGVI 146
           GFS RLT+E+  ++  + G IS  PE    LHTTRTP +LGL++H   ++  +     V+
Sbjct: 83  GFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVV 142

Query: 147 IGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCE----FGASICNNKLIGARTFNLANNVLM 206
           +GVLDTG+ P   S++DEG    P+ WKG CE    F AS+CN KLIGAR F       M
Sbjct: 143 VGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTM 202

Query: 207 G--------KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCS 266
           G        +SP D++GHGTHT+STAAG+ V+GA  LG A G A GMAP A +A+YKVC 
Sbjct: 203 GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW 262

Query: 267 QKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFKDAIAIGAFAAIKKGIFVSCSAGN 326
             GC SSDILAA+D AI D V+VLS+SLG     +++D +AIGAFAA+++GI VSCSAGN
Sbjct: 263 LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGN 322

Query: 327 SGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFLPLVYA 386
           +GPS S+L+N APWI TVGA T+DR   ALA LGNGK +TG SLF+    P K LP +YA
Sbjct: 323 AGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA 382

Query: 387 GK-SGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILINQKPD 446
           G  S       C+ G+L    V GK+V+C+RG   +R+ KG VVK  GG  MIL N   +
Sbjct: 383 GNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAAN 442

Query: 447 GFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAMASFS 506
           G   +A+AH+LP T +  + G  I+ Y+ +  NP ASIS  GT+ G +    SP +A+FS
Sbjct: 443 GEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP---SPVVAAFS 502

Query: 507 SRGPCQASPGILKPDITGPGVNILAAW-----PFPLNNNTNTKSTFNVISGTSMSCPHLS 566
           SRGP   +P ILKPD+  PGVNILAAW     P  L +++  +  FN+ISGTSMSCPH+S
Sbjct: 503 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR-RVEFNIISGTSMSCPHVS 562

Query: 567 GIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKA 626
           G+AAL+KSVHP WSPAAI+SA+MT+A      GKP++D    KP+  F  G+GHV+P+ A
Sbjct: 563 GLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTA 622

Query: 627 ANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVLRIREGDLNYPSFAVSL-G 686
            NPGL+YD+  +DY+ +LC L YT  Q+  + RR  TC         DLNYPSFAV++ G
Sbjct: 623 TNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDG 682

Query: 687 AGSQTFNRTVTNVGDANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSVTFSRNDFV 746
            G+  + RTVT+VG A +    +     G+ + V P  L F + NEK +Y+VTF+  D  
Sbjct: 683 VGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSS 742

Query: 747 RTTSEFSEGYLIWVSNKHMVRSPISVKLT 753
           + +   S G + W   KH+V SP+++  T
Sbjct: 743 KPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of PI0016253 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 616.3 bits (1588), Expect = 3.2e-176
Identity = 365/784 (46.56%), Postives = 482/784 (61.48%), Query Frame = 0

Query: 5   PFF-CICFFLNSHGYVAMKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLP 64
           PFF CI F L            SS+     QTYIV +  P            +W+ SFL 
Sbjct: 5   PFFLCIIFLLFCS---------SSSEILQKQTYIVQL-HPNSETAKTFASKFDWHLSFLQ 64

Query: 65  ETI----EASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTT 124
           E +    E      SRLLYSY   + GF+A+LT+ + + +      ++  P+ +L + TT
Sbjct: 65  EAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTT 124

Query: 125 RTPEYLGLN--QHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCE- 184
            + ++LGL+   + GVW  S FG+G IIGVLDTG+ P  PSF+D GM S P KWKG C+ 
Sbjct: 125 YSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQE 184

Query: 185 ---FGASICNNKLIGARTFNLANNVLMG--KSPN---------DENGHGTHTASTAAGTF 244
              F +S CN KLIGAR F   + V     +SPN         D  GHGTHTAST  G+ 
Sbjct: 185 GESFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSS 244

Query: 245 VKGAEALGNARGKAVGMAPLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGA 304
           V  A  LGN  G A GMAP AHIA+YKVC   GC SSDILAA+D AI D VDVLSLSLG 
Sbjct: 245 VSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGG 304

Query: 305 PSIPFFKDAIAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVAL 364
             IP + D IAIG F A+++GI V C+AGN+GP +S++AN APW+ T+GA T+DR+  A+
Sbjct: 305 FPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAV 364

Query: 365 AKLGNGKVYTGESLFQPRDFPS--KFLPLVYAGKSGIEGSEFCVQGSLEKLNVTGKVVVC 424
            +L NGK+  GESL+  +   +  + + ++Y    G +GSEFC++GSL +  + GK+V+C
Sbjct: 365 VRLANGKLLYGESLYPGKGIKNAGREVEVIYV-TGGDKGSEFCLRGSLPREEIRGKMVIC 424

Query: 425 ERGGGISRIAKGLVVKNGGGAAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYIN 484
           +RG    R  KG  VK  GG AMIL N + +      + H+LP T + Y + + +KAY+N
Sbjct: 425 DRGVN-GRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVN 484

Query: 485 SSHNPKASISFKGTLFGNRAATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPF 544
           ++  PKA I F GT+ G   A   P +A FS+RGP  A+P ILKPD+  PGVNI+AAWP 
Sbjct: 485 ATVKPKARIIFGGTVIGRSRA---PEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQ 544

Query: 545 PLNNN----TNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRN 604
            L        + +  F V+SGTSMSCPH+SGI ALI+S +PNWSPAAIKSA+MT+AD+ +
Sbjct: 545 NLGPTGLPYDSRRVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYD 604

Query: 605 PQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSII 664
            QGK I D + KPA  FA+G+GHVNP KA NPGLVY+IQP DY+ YLC L +T + +  I
Sbjct: 605 RQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAI 664

Query: 665 VRRQVTCSTVLRIREG-DLNYPSFAVSLGAGSQT--FNRTVTNVGDANSVYSAIVKAPVG 724
             + V+C+ +LR   G  LNYPS AV    G  T    R VTNVG  NS+YS  VKAP G
Sbjct: 665 THKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEG 724

Query: 725 ISVRVTPRNLKFSKVNEKVTYSVTF--SRNDFVRTTSEFSEGYLIWVSNKHM---VRSPI 752
           I V V P+ L F  V++ ++Y V F   + +     + F++G L WV++ ++   VRSPI
Sbjct: 725 IKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPI 772

BLAST of PI0016253 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 590.9 bits (1522), Expect = 1.5e-168
Identity = 350/758 (46.17%), Postives = 460/758 (60.69%), Query Frame = 0

Query: 26  SSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLPETIEASSNEQSRLLYSYQHVMS 85
           SS+S+  L++YIVHV++    +L  + +  NW+ S L      SS + + LLYSY   + 
Sbjct: 23  SSSSSDGLESYIVHVQRSHKPSLFSSHN--NWHVSLLRSL--PSSPQPATLLYSYSRAVH 82

Query: 86  GFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPEYLGLNQHFGVWKNSNFGKGVII 145
           GFSARL+  Q  A+      IS  P+    +HTT TP +LG +Q+ G+W NSN+G+ VI+
Sbjct: 83  GFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIV 142

Query: 146 GVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFG----ASICNNKLIGARTF--------- 205
           GVLDTGI P HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F         
Sbjct: 143 GVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRN 202

Query: 206 -NLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVC 265
               +     +SP D  GHGTHTASTAAG+ V  A     ARG A GMA  A IA YK+C
Sbjct: 203 GTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKIC 262

Query: 266 SQKGCSSSDILAALDAAIDDGVDVLSLSLGAP-SIP-FFKDAIAIGAFAAIKKGIFVSCS 325
              GC  SDILAA+D A+ DGV V+SLS+GA  S P +  D+IAIGAF A + GI VSCS
Sbjct: 263 WTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCS 322

Query: 326 AGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGKVYTGESLFQPRDFPSKFLPL 385
           AGNSGP+  T  N APWILTVGAST+DR+  A A  G+GKV+TG SL+     P   L L
Sbjct: 323 AGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSL 382

Query: 386 VYAGKSGIEGSEFCVQGSLEKLNVTGKVVVCERGGGISRIAKGLVVKNGGGAAMILINQK 445
           VY+G     GS  C  G L    V GK+V+C+RGG  +R+ KG  VK  GGA MIL N  
Sbjct: 383 VYSGDC---GSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTA 442

Query: 446 PDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLFGNRAATFSPAMAS 505
             G    A++H++P T +  + G +I+ YI +S +P A ISF GTL G   +  SP +A+
Sbjct: 443 ESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG--PSPPSPRVAA 502

Query: 506 FSSRGPCQASPGILKPDITGPGVNILAAW-----PFPLNNNTNTKSTFNVISGTSMSCPH 565
           FSSRGP   +P ILKPD+  PGVNILA W     P  L+ +   +  FN+ISGTSMSCPH
Sbjct: 503 FSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPH 562

Query: 566 LSGIAALIKSVHPNWSPAAIKSAIMTSA-DVRNPQGKPIVDQDL-KPANFFAMGSGHVNP 625
           +SG+AAL++  HP+WSPAAIKSA++T+A DV N  G+PI D    K +N F  G+GHV+P
Sbjct: 563 VSGLAALLRKAHPDWSPAAIKSALVTTAYDVEN-SGEPIEDLATGKSSNSFIHGAGHVDP 622

Query: 626 SKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVT----CSTVLRIREGDLNYPSF 685
           +KA NPGLVYDI+  +YV +LC +  +    ++  +  T    C T      GDLNYPSF
Sbjct: 623 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 682

Query: 686 AVSLGAGSQT--FNRTVTNVG-DANSVYSAIVKAPVGISVRVTPRNLKFSKVNEKVTYSV 745
           +V   +  +   + R V NVG + ++VY   VK+P  + + V+P  L FSK    + Y V
Sbjct: 683 SVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEV 742

Query: 746 TFSR---NDFVRTTSEFSEGYLIWVSNKHMVRSPISVK 751
           TF        V +      G + W   +H+V+SP++V+
Sbjct: 743 TFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQ 768

BLAST of PI0016253 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 588.6 bits (1516), Expect = 7.2e-168
Identity = 344/776 (44.33%), Postives = 471/776 (60.70%), Query Frame = 0

Query: 1   MVFLPFFCICFFLNSHGYVAMKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTS 60
           M+F   F + FFL            +S  ++  QTY++H       ++     + + + S
Sbjct: 18  MMFFRSFIVFFFL---------IFFASNVSSRKQTYVIHTVTTSTKHI-----VTSLFNS 77

Query: 61  FLPETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTR 120
              E I         + Y Y++ MSGFSA LT +Q+  ++   GFISA P+ +L+LHTT 
Sbjct: 78  LQTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTY 137

Query: 121 TPEYLGLNQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCE---- 180
           + E+LGL    G+W  ++    VIIG++DTGI P H SF D  M+  P++W+G C+    
Sbjct: 138 SHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTN 197

Query: 181 FGASICNNKLIGARTFNLANNVLMGK--------SPNDENGHGTHTASTAAGTFVKGAEA 240
           F +S CN K+IGA  F      ++GK        S  D  GHGTHTASTAAG  V  A  
Sbjct: 198 FSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANY 257

Query: 241 LGNARGKAVGMAPLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFF 300
            G A+G A GM   + IA YK C   GC+S+D++AA+D AI DGVDV+SLSLG  S PF+
Sbjct: 258 FGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFY 317

Query: 301 KDAIAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNG 360
            D IAI  F A++K IFVSCSAGNSGP+ ST++N APW++TV AS  DR   A+ ++GN 
Sbjct: 318 VDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNR 377

Query: 361 KVYTGESLFQPRDFPSKFLPLVYAGKSGIE-GSEFCVQGSLEKLNVTGKVVVCERGGGIS 420
           K   G SL++ +    K LPL +   +G E G+ FC++ SL++  V GK+V+C RG    
Sbjct: 378 KSLVGSSLYKGKSL--KNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS-G 437

Query: 421 RIAKGLVVKNGGGAAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKA 480
           R AKG  VK  GGAAM+L++ + +G   LA+ HVLP   L + DG  +  Y+  + N  A
Sbjct: 438 RTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATA 497

Query: 481 SISFKGTLFGNRAATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAW-PF---PLN 540
           S+ F+GT +G  A    P +A+FSSRGP  A P I KPDI  PG+NILA W PF    L 
Sbjct: 498 SVRFRGTAYGATA----PMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLL 557

Query: 541 NNTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNPQGKPIV 600
            +   +  FN+ISGTSM+CPH+SGIAALIKSVH +WSPA IKSAIMT+A + + + +PI 
Sbjct: 558 RSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIG 617

Query: 601 DQDL----KPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRR 660
           D+        A  FA G+G+V+P++A +PGLVYD    DY+ YLC L YT  ++ +    
Sbjct: 618 DRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGT 677

Query: 661 QVTC-STVLRIREGDLNYPSFAVSL--GAGSQT--FNRTVTNVGDANSVYSAIVKAPVGI 720
             TC S  + +  GDLNYPSFAV+L  GA  +T  + RTVTNVG     Y   V+ P G+
Sbjct: 678 NYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGV 737

Query: 721 SVRVTPRNLKFSKVNEKVTYSVTFSRNDFVRTTSEFSEGYLIWVSNKHMVRSPISV 750
            VRV P+ LKF K  E+++Y+VT+   +  R +S  S G L+W+ +K+ VRSPI+V
Sbjct: 738 KVRVEPKVLKFQKARERLSYTVTYDA-EASRNSSSSSFGVLVWICDKYNVRSPIAV 771

BLAST of PI0016253 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 583.9 bits (1504), Expect = 1.8e-166
Identity = 344/786 (43.77%), Postives = 472/786 (60.05%), Query Frame = 0

Query: 4   LPFFCICFFLNSHGYVAMKTELSSTSNTNLQTYIVHVKQPELGNLGDAIDLQNWYTSFLP 63
           + FF   FFL     + + +  SS S++N  TYIVHV      ++       +WYTS   
Sbjct: 1   MAFFFYFFFL-----LTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPT--HFHWYTS--- 60

Query: 64  ETIEASSNEQSRLLYSYQHVMSGFSARLTKEQVKAMEEKDGFISATPETILNLHTTRTPE 123
            ++ + ++    ++++Y  V  GFSARLT +    + +    IS  PE + +LHTTR+PE
Sbjct: 61  -SLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPE 120

Query: 124 YLGL--NQHFGVWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRC----EF 183
           +LGL      G+ + S+FG  ++IGV+DTG+ P  PSF+D G+   P KWKG+C    +F
Sbjct: 121 FLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDF 180

Query: 184 GASICNNKLIGARTF--------NLANNVLMGKSPNDENGHGTHTASTAAGTFVKGAEAL 243
             S CN KL+GAR F           N     +SP D +GHGTHTAS +AG +V  A  L
Sbjct: 181 PESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTL 240

Query: 244 GNARGKAVGMAPLAHIAMYKVCSQKGCSSSDILAALDAAIDDGVDVLSLSLGAPSIPFFK 303
           G A G A GMAP A +A YKVC   GC  SDILAA D A+ DGVDV+SLS+G   +P++ 
Sbjct: 241 GYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYL 300

Query: 304 DAIAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRKIVALAKLGNGK 363
           DAIAIGAF AI +GIFVS SAGN GP   T+ N APW+ TVGA TIDR   A  KLGNGK
Sbjct: 301 DAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGK 360

Query: 364 VYTGESLF-QPRDFPSKFLPLVYAGK-SGIEG--SEFCVQGSLEKLNVTGKVVVCERGGG 423
           + +G S++  P   P +  PLVY G   G +G  S  C++GSL+   V GK+V+C+RG  
Sbjct: 361 MISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGIN 420

Query: 424 ISRIAKGLVVKNGGGAAMILINQKPDGFSTLAEAHVLPTTHLSYEDGLKIKAYIN----- 483
            SR  KG +V+  GG  MI+ N   DG   +A+ HVLP T +    G +I+ YI+     
Sbjct: 421 -SRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKS 480

Query: 484 -SSHNPKASISFKGTLFGNRAATFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWP 543
            SS +P A+I FKGT  G R A   P +ASFS+RGP   +P ILKPD+  PG+NILAAWP
Sbjct: 481 RSSKHPTATIVFKGTRLGIRPA---PVVASFSARGPNPETPEILKPDVIAPGLNILAAWP 540

Query: 544 FPLN----NNTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVR 603
             +      + N ++ FN++SGTSM+CPH+SG+AAL+K+ HP+WSPAAI+SA++T+A   
Sbjct: 541 DRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTV 600

Query: 604 NPQGKPIVDQDL-KPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVS 663
           +  G+P++D+     ++    GSGHV+P+KA +PGLVYDI   DY+ +LC+  YT   + 
Sbjct: 601 DNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIV 660

Query: 664 IIVRRQVTCSTVLRI-REGDLNYPSFAVSL-----GAGSQTFNRTVTNVGDANSVYSAIV 723
            I RRQ  C    R    G+LNYPSF+V          S  F RTVTNVGD++SVY   +
Sbjct: 661 TITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKI 720

Query: 724 KAPVGISVRVTPRNLKFSKVNEKVTYSVTFSRNDFVRT--TSEFSEGYLIWVSNKHMVRS 752
           + P G +V V P  L F +V +K+++ V     +   +   +    G+++W   K  V S
Sbjct: 721 RPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTS 771