MS018728 (gene) Bitter gourd (TR) v1

BLAST of MS018728 vs. NCBI nr
Match: XP_022146394.1 (DNA-directed RNA polymerase V subunit 1 [Momordica charantia])

HSP 1 Score: 3900.9 bits (10115), Expect = 0.0e+00
Identity = 1938/1947 (99.54%), Postives = 1939/1947 (99.59%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL
Sbjct: 89   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD
Sbjct: 149  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV
Sbjct: 209  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR
Sbjct: 449  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
            TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPA FDCHGDSYLIKN
Sbjct: 509  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPAYFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY
Sbjct: 569  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL
Sbjct: 689  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK
Sbjct: 809  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY
Sbjct: 869  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS
Sbjct: 929  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
            FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA
Sbjct: 989  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR
Sbjct: 1049 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEE TSACLGEEI+DLMVEDEY ELTLS
Sbjct: 1229 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEEFTSACLGEEIEDLMVEDEYRELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTG GQWENNENTKATNSSN
Sbjct: 1289 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGSGQWENNENTKATNSSN 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
            DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD
Sbjct: 1349 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFNKKI 1440
            SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAF KKI
Sbjct: 1409 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFIKKI 1468

Query: 1441 NSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNSGD 1500
            NSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNSGD
Sbjct: 1469 NSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNSGD 1528

Query: 1501 ADQSEACRNDGQASMDLETVADRWGSMATQRKDSKDNFPSKAVEHGDSPLINHSWNQHKS 1560
            ADQSEACRND QASMDLETVADRWGS ATQRKDSKDNFPSKAVEHGDSPLINHSWNQHKS
Sbjct: 1529 ADQSEACRNDDQASMDLETVADRWGSRATQRKDSKDNFPSKAVEHGDSPLINHSWNQHKS 1588

Query: 1561 SEVFRGESGNDFWGQRKSQDVIKPSQGWGSQVKSNEGSSQNTQVERLWSSQNESDQVASE 1620
            SEVFRGESGNDFWGQRKSQDVIKPSQGWGSQVKSNEGSSQNTQVERLWSSQNESDQVASE
Sbjct: 1589 SEVFRGESGNDFWGQRKSQDVIKPSQGWGSQVKSNEGSSQNTQVERLWSSQNESDQVASE 1648

Query: 1621 HKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSN 1680
            HKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSN
Sbjct: 1649 HKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSN 1708

Query: 1681 KQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSHSWGSHKESSELTTSHA 1740
            KQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSH WGSHKESSELTTSHA
Sbjct: 1709 KQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSHGWGSHKESSELTTSHA 1768

Query: 1741 WEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDIL 1800
            WEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDIL
Sbjct: 1769 WEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDIL 1828

Query: 1801 ADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRH 1860
            ADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRH
Sbjct: 1829 ADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRH 1888

Query: 1861 SSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRSGKPRDRNS 1920
            SSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRSGKPRDRNS
Sbjct: 1889 SSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRSGKPRDRNS 1948

Query: 1921 ASEENENKNVGKELTPIPEETENGNQQ 1948
            ASEENENKNVGKELTPIPEETENGNQQ
Sbjct: 1949 ASEENENKNVGKELTPIPEETENGNQQ 1975

BLAST of MS018728 vs. NCBI nr
Match: XP_038874337.1 (DNA-directed RNA polymerase V subunit 1 [Benincasa hispida])

HSP 1 Score: 3406.3 bits (8831), Expect = 0.0e+00
Identity = 1716/1992 (86.14%), Postives = 1801/1992 (90.41%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPI+HPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIYHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TELKKMLSLLCLKCLKMKK KFPSKNIGFAERLLS+CCEDASQVSIRE KKADGASYLQL
Sbjct: 89   TELKKMLSLLCLKCLKMKKTKFPSKNIGFAERLLSACCEDASQVSIREAKKADGASYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRT LREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNE RKKLAG+GY PQD
Sbjct: 149  KVPSRTSLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNETRKKLAGRGYCPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYILQYLPVPPNCLSVPEISDGVT+MSSDPAVSMLKKILKQVEII+GSRSGAPNFESHEV
Sbjct: 209  GYILQYLPVPPNCLSVPEISDGVTVMSSDPAVSMLKKILKQVEIIRGSRSGAPNFESHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNI YLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNIKYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQL  DSLLSLKMMFR
Sbjct: 449  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLANDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
             YFL KAAAQQLAMFVSS LP PA+LG RS S HWTALQILQTVLPACFDCHGDSYLIKN
Sbjct: 509  KYFLDKAAAQQLAMFVSSYLPPPALLGVRSGSLHWTALQILQTVLPACFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            SDFLKFDFDRDAMPSLINEI+TSIFFQ GPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY
Sbjct: 569  SDFLKFDFDRDAMPSLINEILTSIFFQKGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMA LQALQKNIQVISPLLYQLRSTFNELVELQLENHIR+VKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMAFLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRAVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKSDSA+NKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRY SDKIDYPSAEFGL
Sbjct: 689  FDSKSDSAVNKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYSSDKIDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYG+KAGMMKP++LFPPGEPVGVLAATAMSNPAYKAVLDSTPSS SSWDMMK
Sbjct: 809  CSNSIIQLEYGMKAGMMKPYSLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSNSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKVSFKNEPIDRRVILYLNNCACGRK+CNENAAY+VKSHLKKVTLKDA VDFMIEY
Sbjct: 869  EILLCKVSFKNEPIDRRVILYLNNCACGRKYCNENAAYVVKSHLKKVTLKDAAVDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQ T SG GPGLVGHVHLNKMLLKELKI+M +V RRC+ETISSFR KKKKK AHALRFS
Sbjct: 929  NRQPTPSGLGPGLVGHVHLNKMLLKELKIDMTEVLRRCQETISSFR-KKKKKIAHALRFS 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
             SE CSFHQ NGE+STDMPCLIFWHETRD HLERTAHI AD+VFPLLSETIIKGDPRIS+
Sbjct: 989  ISEQCSFHQWNGEESTDMPCLIFWHETRDVHLERTAHILADVVFPLLSETIIKGDPRISS 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            ANVIWISPDSTSWQ+NPSRWQDGELALD+CLEKSAVKQNGDAWRNV+DCCLPVIHLIDTR
Sbjct: 1049 ANVIWISPDSTSWQKNPSRWQDGELALDVCLEKSAVKQNGDAWRNVLDCCLPVIHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RS+PYAIKQVQ+LLGISCAFDQ +QRL+KSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSVPYAIKQVQDLLGISCAFDQMIQRLSKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPR+CFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRKCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            RFDILWDQKELGCKQDDV+DVYNFLHMVRS+KSEE TSACLGEEI+D+MVEDEYGELTLS
Sbjct: 1229 RFDILWDQKELGCKQDDVVDVYNFLHMVRSSKSEEPTSACLGEEIEDIMVEDEYGELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEP  TSEKPVFEDSAEFE+CLDNYPGESKWEKAP  GA STGGGQWENNEN KATNSS+
Sbjct: 1289 PEPL-TSEKPVFEDSAEFEHCLDNYPGESKWEKAPSLGAVSTGGGQWENNENGKATNSSD 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
            D+DWSGWGRK EPDV  T AQENTS S WD+TPSWGNKATN T+NDNDWSNS TKEVE D
Sbjct: 1349 DNDWSGWGRKAEPDVANTNAQENTSNSAWDTTPSWGNKATN-TSNDNDWSNSGTKEVERD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFNKKI 1440
            SF SME TPKSGGWDTA+TWGTK KDVD FKG+T PEK+N+WSG QN+KAETQDAF+KK+
Sbjct: 1409 SFTSMEKTPKSGGWDTASTWGTKTKDVDGFKGDTAPEKSNLWSGLQNEKAETQDAFHKKV 1468

Query: 1441 --NSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNS 1500
               S+S G EDKAWS G+SKT DNWS+QVKDKAES QVQVQEV SKTNGW SAG W+KNS
Sbjct: 1469 EMTSKSRGWEDKAWSRGSSKTEDNWSSQVKDKAESFQVQVQEVSSKTNGWGSAGSWRKNS 1528

Query: 1501 GDADQSEACRNDGQASMDLETVADRWGSMATQRK--------------DSKDNFPSKAVE 1560
            GD  QSEA  NDGQASMDL+ V+DRW S AT R               DSKD+FPSKAVE
Sbjct: 1529 GDDHQSEAGWNDGQASMDLDKVSDRWDSRATDRMESQRTSSWGSQTVCDSKDSFPSKAVE 1588

Query: 1561 HGDSPLINHSWNQHKSSEVFRGESGNDFWGQRKSQDVIKPS--------QGWGSQVKSNE 1620
            H D+ ++NHSW+QHKS E  +G  GND WGQ+KS++VIKPS        +GWGSQ++SNE
Sbjct: 1589 HSDA-VLNHSWDQHKSPEASQG-FGNDVWGQQKSREVIKPSHVNNESNQRGWGSQIESNE 1648

Query: 1621 GSSQNTQVERLWSSQNESDQVASEHKSSDSRGWDSQEKLNKPWDK--------------- 1680
            GS                DQV SEHKSSD+ GWDSQEK++KPWDK               
Sbjct: 1649 GSGHG------------FDQVTSEHKSSDTGGWDSQEKMDKPWDKQKSTEASQSWGSQEK 1708

Query: 1681 -------QKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSNKQFSQVQKSPEVSH 1740
                   QKS EASQSW SQNDS+GSWGQ QR +EEFS+GSQDDSN QFSQ+ K PE S 
Sbjct: 1709 MDKPWDTQKSTEASQSWGSQNDSLGSWGQPQRAAEEFSRGSQDDSNTQFSQL-KPPETSL 1768

Query: 1741 GWGSHKESSELTTSHGWGSHKESSELATSHSWGSHKESSELTTSHAWEKKNQGSKGWGAN 1800
            GW                   E      SH WGSHKESSE T+SH W+KKNQGSKGWG N
Sbjct: 1769 GW-------------------EQKSPEVSHGWGSHKESSEQTSSHGWDKKNQGSKGWGGN 1828

Query: 1801 VGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDILADIEPIMQSIRKIM 1860
             GEWKNRKNRPPKSPG+LNDD+ LRAI+TASGQRLDMFTTEEQDILADIEPIMQSIRK+M
Sbjct: 1829 AGEWKNRKNRPPKSPGVLNDDSNLRAIFTASGQRLDMFTTEEQDILADIEPIMQSIRKVM 1888

Query: 1861 HQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRHSSFQESRCFYVVST 1920
            HQSGYNDGDPLSAEDQSF+LQ+VFNFHPDKA KMGAGIDHFMVSRHSSFQESRCFYVV+T
Sbjct: 1889 HQSGYNDGDPLSAEDQSFVLQSVFNFHPDKAAKMGAGIDHFMVSRHSSFQESRCFYVVTT 1948

Query: 1921 DGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRSGKPRDRNSASEENENKNVGKEL 1947
            DGHKEDFSYRKCLDNF+KGKYPD+AE FVAKYFRKPR  + RDRNSASEENENKN+G EL
Sbjct: 1949 DGHKEDFSYRKCLDNFIKGKYPDLAEMFVAKYFRKPRPNRNRDRNSASEENENKNIGGEL 1983

BLAST of MS018728 vs. NCBI nr
Match: XP_011655250.1 (DNA-directed RNA polymerase V subunit 1 [Cucumis sativus] >XP_031741011.1 DNA-directed RNA polymerase V subunit 1 [Cucumis sativus] >XP_031741012.1 DNA-directed RNA polymerase V subunit 1 [Cucumis sativus] >XP_031741013.1 DNA-directed RNA polymerase V subunit 1 [Cucumis sativus] >XP_031741014.1 DNA-directed RNA polymerase V subunit 1 [Cucumis sativus] >KGN51090.1 hypothetical protein Csa_009187 [Cucumis sativus])

HSP 1 Score: 3345.8 bits (8674), Expect = 0.0e+00
Identity = 1683/1971 (85.39%), Postives = 1783/1971 (90.46%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPI+HPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIYHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TELKKMLSLLCLKCLKMKK KFPSKNIGFAERLLSSCCEDASQV+IRE KKADGASYLQL
Sbjct: 89   TELKKMLSLLCLKCLKMKKTKFPSKNIGFAERLLSSCCEDASQVTIREAKKADGASYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRT L+E FWDFLERYGFRYGDN TRTLLPCEVKEMLKKIPNE RKKLAG+GYYPQD
Sbjct: 149  KVPSRTSLQERFWDFLERYGFRYGDNFTRTLLPCEVKEMLKKIPNETRKKLAGRGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYILQYLPVPPNCLSVPEISDGVT+MSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV
Sbjct: 209  GYILQYLPVPPNCLSVPEISDGVTVMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQLAVDQYLQVRGTVKASRGIDAR+GVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQLAVDQYLQVRGTVKASRGIDARFGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNI YLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNIRYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDH VKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHVVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQL  DSLLSLKMMFR
Sbjct: 449  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLANDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
             YFLGKAAAQQLAMFVSS LP PA+LG RS S HWTALQILQTVLPA FDCHGDSYLIKN
Sbjct: 509  KYFLGKAAAQQLAMFVSSYLPPPALLGVRSGSLHWTALQILQTVLPASFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            S+FLKFDFDRDAMPSLINEI+TSIFFQ GPEEVL+FFDSLQPLLMEHIFSEGFSVGLDDY
Sbjct: 569  SNFLKFDFDRDAMPSLINEILTSIFFQKGPEEVLKFFDSLQPLLMEHIFSEGFSVGLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMA LQALQKNIQV+SPLLYQLRSTFNELVELQLENH+RSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMAFLQALQKNIQVLSPLLYQLRSTFNELVELQLENHLRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKS+SAINKVVQQIGFLGLQLSDKG+FYSK+LIEDVASLFHNRY SDKIDYPSAEFGL
Sbjct: 689  FDSKSESAINKVVQQIGFLGLQLSDKGRFYSKSLIEDVASLFHNRYSSDKIDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYG+KAGMM+P++LFPPGEPVGVLAATAMS PAYKAVLDSTPSS SSWDMMK
Sbjct: 809  CSNSIIQLEYGMKAGMMQPYSLFPPGEPVGVLAATAMSTPAYKAVLDSTPSSNSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKVSFKNEPIDRRVILYLNNCACGRK+CNENAAY+VKSHLKKVTLKDA +DFMIEY
Sbjct: 869  EILLCKVSFKNEPIDRRVILYLNNCACGRKYCNENAAYVVKSHLKKVTLKDAAMDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQ T SG GPGLVGHVHLN+MLLKEL I+M +V RRC+ET+SSF KKKKKK AHALRFS
Sbjct: 929  NRQPTPSGLGPGLVGHVHLNRMLLKELNIDMTEVLRRCQETMSSF-KKKKKKIAHALRFS 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
             SE+C+FHQ NGE+S DMPCLIFWH+TRD HLERTAHI ADIVFPLLSETIIKGDPRI +
Sbjct: 989  ISEHCAFHQWNGEESIDMPCLIFWHQTRDVHLERTAHILADIVFPLLSETIIKGDPRIKS 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            A+VIWISPDSTSWQ+NPSRWQDGELALD+CLEKSAVKQNGDAWRNV+DCCLPV+HLIDTR
Sbjct: 1049 ASVIWISPDSTSWQKNPSRWQDGELALDVCLEKSAVKQNGDAWRNVLDCCLPVLHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RS+PYAIKQVQELLGISCAFDQ +QRL+KSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSVPYAIKQVQELLGISCAFDQMIQRLSKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPR+CFE+AAEKCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRKCFEKAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            RFDILWDQKELGCKQDDV+DVYNFLHMVRS KSEE TSACLGEEI+D+MVEDEYGELTLS
Sbjct: 1229 RFDILWDQKELGCKQDDVVDVYNFLHMVRSGKSEEPTSACLGEEIEDIMVEDEYGELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEPFSTSEKPVFEDSAEFE+CLDNYPGESKWEKAP  GA STGGGQWE+NEN KATNSS+
Sbjct: 1289 PEPFSTSEKPVFEDSAEFEHCLDNYPGESKWEKAPSLGAVSTGGGQWESNENGKATNSSD 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
             +DWSGWGRK EPDV  T AQENTS S WD+T SWGNKATN ++NDNDWSN +TKEVE D
Sbjct: 1349 GNDWSGWGRKAEPDVTVTNAQENTSNSAWDTTSSWGNKATN-SSNDNDWSNCSTKEVERD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFNKK- 1440
            SF SME TPKSGGWD+A+TWGTK KD D+FK ET P+K++ WSG Q DKAETQDAF+KK 
Sbjct: 1409 SFTSMEKTPKSGGWDSASTWGTKTKD-DSFKRETAPKKSSQWSGLQKDKAETQDAFHKKA 1468

Query: 1441 -INSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNS 1500
             + S+S G EDKAWS GTSKT DNWS+QVKDKAES QVQVQEV SKTNGW S GGW KNS
Sbjct: 1469 EMASKSGGWEDKAWSRGTSKTEDNWSSQVKDKAESFQVQVQEVSSKTNGWGSTGGWTKNS 1528

Query: 1501 GDADQSEACRNDGQASMDLETVADRWGSMATQR--------------KDSKDNFPSKAVE 1560
            G   QSEA  NDGQASMD E V+DRW   ATQ+               DSKD+FPSKAV+
Sbjct: 1529 GGDHQSEAGWNDGQASMDREKVSDRWDRKATQKLESHQTSSWGSPTVGDSKDSFPSKAVD 1588

Query: 1561 HGDSPLINHSWNQHKSSEVFRGESGNDFWGQRKSQDVIKPS--------QGWGSQVKSNE 1620
            H DS ++NHSW++ KS E  +G  GND WGQ+KS+DVIKPS         GWGSQ++SNE
Sbjct: 1589 HSDS-VVNHSWDRQKSPEASQG-FGNDAWGQQKSRDVIKPSLANNESNLSGWGSQIESNE 1648

Query: 1621 GSSQNTQVERLWSSQNESDQVASEHKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQND 1680
            GS                DQV +E KSSD+RGWDSQEK +KPWDKQKSLEASQSW SQND
Sbjct: 1649 GSDHG------------FDQVTNEQKSSDTRGWDSQEKTDKPWDKQKSLEASQSWGSQND 1708

Query: 1681 SMGSWGQLQRESEEFSQGSQDDSNKQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKE 1740
            S+GSWGQ QR SEE S+ SQDDS+ QFSQ+ K PE S GW   K                
Sbjct: 1709 SLGSWGQPQRASEECSRESQDDSSTQFSQL-KPPETSLGWEQQKSPE------------- 1768

Query: 1741 SSELATSHSWGSHKESSELTTSHAWEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDA 1800
                  SH WGS+KESSE T+SH W+KKNQGSKGWG N GEWKNRKNRPPKSPG+ NDDA
Sbjct: 1769 -----VSHGWGSNKESSEQTSSHGWDKKNQGSKGWGGNAGEWKNRKNRPPKSPGMSNDDA 1828

Query: 1801 GLRAIYTASGQRLDMFTTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQN 1860
             LRA+YTASGQRLDMFT+EEQDILADIEPIMQSIRK+MHQSGYNDGDPLSAEDQSF+LQ+
Sbjct: 1829 NLRALYTASGQRLDMFTSEEQDILADIEPIMQSIRKVMHQSGYNDGDPLSAEDQSFVLQS 1888

Query: 1861 VFNFHPDKAVKMGAGIDHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYP 1920
            VFNFHPDKA KMGAGIDHFMVSRHSSFQESRCFYVV+TDGHKEDFSYRKCLDNF+KGKYP
Sbjct: 1889 VFNFHPDKAAKMGAGIDHFMVSRHSSFQESRCFYVVTTDGHKEDFSYRKCLDNFIKGKYP 1948

Query: 1921 DIAEPFVAKYFRKPRSGKPRDRNSASEENENKNVGKELTPIPEETENGNQQ 1948
            D+AE FVAKYFRKPR  + RDRN ASEENENK++G ELTPIPEE +NG+QQ
Sbjct: 1949 DLAEMFVAKYFRKPRPNRNRDRNPASEENENKSIGGELTPIPEEAQNGSQQ 1963

BLAST of MS018728 vs. NCBI nr
Match: XP_008465860.1 (PREDICTED: DNA-directed RNA polymerase V subunit 1 [Cucumis melo])

HSP 1 Score: 3343.1 bits (8667), Expect = 0.0e+00
Identity = 1681/1971 (85.29%), Postives = 1777/1971 (90.16%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPI+HPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIYHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TEL+KMLSLLCLKCLKMKK KFPSKNIGFAERLLSSCCEDASQV+IRE KKADGASYLQL
Sbjct: 89   TELRKMLSLLCLKCLKMKKTKFPSKNIGFAERLLSSCCEDASQVTIREAKKADGASYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRT L+E FWDFLERYGFRYGDN TRTLLPCEVKEMLKKIPNE RKKLAG+GYYPQD
Sbjct: 149  KVPSRTSLQERFWDFLERYGFRYGDNFTRTLLPCEVKEMLKKIPNETRKKLAGRGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYILQYLPVPPNCLSVPEISDGVT+MSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV
Sbjct: 209  GYILQYLPVPPNCLSVPEISDGVTVMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQLAVDQYLQVRGTVKASRGIDAR+GVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQLAVDQYLQVRGTVKASRGIDARFGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNI YLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNIRYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDH VKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHVVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
             LSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQL  DSLLSLKMMFR
Sbjct: 449  SLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLANDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
             YFLGKAAAQQLAMFVSS LP PA+LG RS S HWTALQILQTVLPACFDCHGDSYLIKN
Sbjct: 509  KYFLGKAAAQQLAMFVSSYLPPPALLGVRSGSLHWTALQILQTVLPACFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            S+FLKFDFD+DAMPSLINEI+TSIFFQ GPEEVL+FFDSLQPLLMEHIFSEGFSVGLDDY
Sbjct: 569  SNFLKFDFDKDAMPSLINEILTSIFFQKGPEEVLKFFDSLQPLLMEHIFSEGFSVGLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMA LQALQKNIQV+SPLLYQLRSTFNELVELQLENH+RSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMAFLQALQKNIQVLSPLLYQLRSTFNELVELQLENHLRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKS+SAINKVVQQIGFLGLQLSDKG+FYSK+LIEDVASLFHNRY SDKIDYPSAEFGL
Sbjct: 689  FDSKSESAINKVVQQIGFLGLQLSDKGRFYSKSLIEDVASLFHNRYSSDKIDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYG+KAGMM+P++LFPPGEPVGVLAATAMS PAYKAVLDSTPSS SSWDMMK
Sbjct: 809  CSNSIIQLEYGMKAGMMQPYSLFPPGEPVGVLAATAMSTPAYKAVLDSTPSSNSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKVSFKNEPIDRRVILYLNNCACGRK+CNENAAY+VKSHLKKVTLKD  VDFMIEY
Sbjct: 869  EILLCKVSFKNEPIDRRVILYLNNCACGRKYCNENAAYVVKSHLKKVTLKDVAVDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQ T SG GPGLVGHVHLN+MLLKEL INM +V RRC+ET+SSF KKKKKK AHALRF+
Sbjct: 929  NRQPTPSGLGPGLVGHVHLNRMLLKELNINMTEVLRRCQETMSSF-KKKKKKVAHALRFA 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
             SE+C+FHQ NG +S DMPCLIFWHETRD HLERTAHI ADIVFPLLSETIIKGDPRI +
Sbjct: 989  ISEHCAFHQWNGVESIDMPCLIFWHETRDVHLERTAHILADIVFPLLSETIIKGDPRIKS 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            A+VIWISPDSTSWQ+NPSRWQDGELALD+CLEKSA+KQNGDAWRNV+DCCLPV+HLIDTR
Sbjct: 1049 ASVIWISPDSTSWQKNPSRWQDGELALDVCLEKSALKQNGDAWRNVLDCCLPVLHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RS+PYAIKQVQELLGISCAFDQ +QRL+KSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSVPYAIKQVQELLGISCAFDQMIQRLSKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPR+CFE+AAEKCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRKCFEKAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            RFDILWDQKELGCKQDDV+DVYNFLHMVRS KSEE TSACLGEE++D+MVEDEYGELTLS
Sbjct: 1229 RFDILWDQKELGCKQDDVVDVYNFLHMVRSGKSEEPTSACLGEEVEDIMVEDEYGELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEPFSTSEKPVFEDSAEFE+CLDN PGESKWEKAP  GA STGGGQWE+N N KAT SS+
Sbjct: 1289 PEPFSTSEKPVFEDSAEFEHCLDNDPGESKWEKAPSLGAVSTGGGQWESNGNGKATKSSD 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
            D+DWSGWGRK EPDV  T AQENTS S WD+T SWGNKAT  T+NDNDWSN +TKEVE D
Sbjct: 1349 DNDWSGWGRKAEPDVTVTNAQENTSNSAWDTTSSWGNKAT-ITSNDNDWSNCSTKEVERD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFNKK- 1440
            SF SME TPKSGGWDTA+TWGTK KD D+F GET PEK+N WS  Q DKAETQDAF+KK 
Sbjct: 1409 SFTSMEKTPKSGGWDTASTWGTKTKD-DSFNGETAPEKSNQWSSLQKDKAETQDAFHKKA 1468

Query: 1441 -INSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNS 1500
             + S+S G EDKAWS GTSKT DNWS QVKDKAES QV VQ+V SKTNGW S GGW KNS
Sbjct: 1469 EMASKSSGWEDKAWSRGTSKTEDNWSGQVKDKAESFQVPVQKVSSKTNGWGSTGGWTKNS 1528

Query: 1501 GDADQSEACRNDGQASMDLETVADRWGSMATQRK--------------DSKDNFPSKAVE 1560
            G   Q+EA  NDGQASMD E  +DRW   ATQ+               DSKD+FPSKAV+
Sbjct: 1529 GGDHQAEAGWNDGQASMDREEASDRWDRKATQKLESHQTSSWGSPTVCDSKDSFPSKAVD 1588

Query: 1561 HGDSPLINHSWNQHKSSEVFRGESGNDFWGQRKSQDVIKPS--------QGWGSQVKSNE 1620
            HGDS ++NHSW++ KS E  +G  GND W Q+KSQDVIKPS         GWGSQ++SNE
Sbjct: 1589 HGDS-VVNHSWDRQKSPEASQG-FGNDAWQQQKSQDVIKPSHANNESNRSGWGSQIESNE 1648

Query: 1621 GSSQNTQVERLWSSQNESDQVASEHKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQND 1680
            GS                DQV SE KSSD+RGWDSQEK++KPWDKQKSLEASQSW SQND
Sbjct: 1649 GSDHG------------FDQVTSEQKSSDTRGWDSQEKMDKPWDKQKSLEASQSWGSQND 1708

Query: 1681 SMGSWGQLQRESEEFSQGSQDDSNKQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKE 1740
            S+GSWGQ QR SEEFS+GSQDDS+ QFSQ+ K PE S GW   K                
Sbjct: 1709 SLGSWGQPQRASEEFSRGSQDDSSTQFSQL-KPPETSLGWEQQKSPE------------- 1768

Query: 1741 SSELATSHSWGSHKESSELTTSHAWEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDA 1800
                  SH WGSHKESSE T+SH W+KKNQGSKGWG N GEWKNRKNRPPKSPG+ +DDA
Sbjct: 1769 -----VSHGWGSHKESSEQTSSHGWDKKNQGSKGWGGNAGEWKNRKNRPPKSPGMSSDDA 1828

Query: 1801 GLRAIYTASGQRLDMFTTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQN 1860
             LRA+YTASGQRLDMFTTEEQDILADIEPIMQSIRK+MHQSGYNDGDPLSAEDQSF+LQ+
Sbjct: 1829 NLRALYTASGQRLDMFTTEEQDILADIEPIMQSIRKVMHQSGYNDGDPLSAEDQSFVLQS 1888

Query: 1861 VFNFHPDKAVKMGAGIDHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYP 1920
            VFNFHPDKA KMGAGIDHFMVSRHSSFQESRCFYVV+TDGHKEDFSYRKCLDNF+KGKYP
Sbjct: 1889 VFNFHPDKAAKMGAGIDHFMVSRHSSFQESRCFYVVTTDGHKEDFSYRKCLDNFIKGKYP 1948

Query: 1921 DIAEPFVAKYFRKPRSGKPRDRNSASEENENKNVGKELTPIPEETENGNQQ 1948
            D+AE FVAKYFRKPR  + RDRN ASEENENK+VG ELTPIPEE +NG+QQ
Sbjct: 1949 DMAEMFVAKYFRKPRPNRNRDRNPASEENENKSVGGELTPIPEEAQNGSQQ 1963

BLAST of MS018728 vs. NCBI nr
Match: XP_022953816.1 (DNA-directed RNA polymerase V subunit 1 [Cucurbita moschata])

HSP 1 Score: 3229.9 bits (8373), Expect = 0.0e+00
Identity = 1647/2015 (81.74%), Postives = 1747/2015 (86.70%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIE+GKCESCGTSEPGKCEGHFGYIELPIPI+HPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEYGKCESCGTSEPGKCEGHFGYIELPIPIYHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TELKKMLSLLCLKCLKMKKNKFPSKN+GFAERLL SCCEDASQVSIRE KK+DGA+YLQL
Sbjct: 89   TELKKMLSLLCLKCLKMKKNKFPSKNVGFAERLL-SCCEDASQVSIREAKKSDGATYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRT LREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNE RKKLAGKGYYPQD
Sbjct: 149  KVPSRTSLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNETRKKLAGKGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GY+LQYLPVPPNCLSVPEISDGVTIMSSDPAV MLKK+LKQVEIIKGSRSGAPNFE+HEV
Sbjct: 209  GYVLQYLPVPPNCLSVPEISDGVTIMSSDPAVLMLKKVLKQVEIIKGSRSGAPNFEAHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQ+AVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQMAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNI YLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNIKYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PL ADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQL  DSLLSLKMMFR
Sbjct: 449  PLGADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLANDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
             YF GKAAAQQLAMFV+SSLP PA+LG RS+S HWTALQILQTVLP+CFDCHGDSYLIKN
Sbjct: 509  KYFFGKAAAQQLAMFVTSSLPPPALLGVRSNSLHWTALQILQTVLPSCFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            SDFLKFDFDRDAMPSLINEIVTSIFFQ GPEEV+RFFDSLQPLLMEH+FSEGFSV LDDY
Sbjct: 569  SDFLKFDFDRDAMPSLINEIVTSIFFQKGPEEVMRFFDSLQPLLMEHVFSEGFSVSLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMA LQALQKNIQVISPLLYQLRS+FNELVELQLENHIRSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMAFLQALQKNIQVISPLLYQLRSSFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKSD+AINKVVQQIGFLGLQLSDKGKFYSKTLI+DVASLFHNRY SDK DYPSAEFGL
Sbjct: 689  FDSKSDAAINKVVQQIGFLGLQLSDKGKFYSKTLIDDVASLFHNRYSSDKNDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYG+KAGMMKP+ LFPPGEPVGVLAATAMS PAYKAVLDSTPSS SSWDMMK
Sbjct: 809  CSNSIIQLEYGIKAGMMKPYGLFPPGEPVGVLAATAMSTPAYKAVLDSTPSSNSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKV FKNEP+DRRVILYLNNC CGRKHCNENAAY+VKSHLKKVTLKD  +DFMIEY
Sbjct: 869  EILLCKVGFKNEPVDRRVILYLNNCDCGRKHCNENAAYVVKSHLKKVTLKDVAMDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQ T S  GPGLVGHVHLN++LL+EL+INMADV RRC+ETISSF KKKKKK A ALRF 
Sbjct: 929  NRQPTPSALGPGLVGHVHLNQVLLEELRINMADVLRRCQETISSF-KKKKKKLAPALRFF 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
             SE+CSFHQ NGE+ TDMPCL FW ETRD HLERT+HI AD+VFPLLSETIIKGDPRIS+
Sbjct: 989  ISEHCSFHQRNGEERTDMPCLTFWLETRDVHLERTSHILADVVFPLLSETIIKGDPRISS 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            ANVIWIS DSTSW+RNPSRWQDGELALD+CLEKSAVK++GDAWRNV+DCCLP+IHLIDTR
Sbjct: 1049 ANVIWISSDSTSWERNPSRWQDGELALDVCLEKSAVKEDGDAWRNVLDCCLPIIHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RS+PYAIKQVQ+LLGISCAFDQT+QRL+KSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSVPYAIKQVQKLLGISCAFDQTIQRLSKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPRRCFERAA KCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRRCFERAATKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            +FDILWDQKELG KQ DV+DVYNFLHMVRS KSEE TSACLG EIDDLMVEDEYGELTLS
Sbjct: 1229 KFDILWDQKELGSKQADVVDVYNFLHMVRSGKSEESTSACLGVEIDDLMVEDEYGELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEPFSTSEKPVFEDSAEFE+CLDN+            GA S GGGQWE+NEN+K   +S 
Sbjct: 1289 PEPFSTSEKPVFEDSAEFEHCLDNH----------SLGAASAGGGQWESNENSK---TSQ 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
            D+DWSGWG KV+PDV        TSKSGWD+TPSWGNKAT   +NDN WS   TKEVE D
Sbjct: 1349 DNDWSGWGTKVDPDV-------TTSKSGWDTTPSWGNKATK-ASNDNGWS---TKEVERD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFK-GETEPEKANVWSGWQNDKAETQDAFNKK 1440
            SF S +NTPK+GGWD+AATWG K KDVD+FK GET PEK+NVWSG Q++KAETQDAF+KK
Sbjct: 1409 SFTSTKNTPKTGGWDSAATWGMKTKDVDSFKEGETAPEKSNVWSGLQSNKAETQDAFHKK 1468

Query: 1441 IN--SRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKN 1500
            +   S+S G +DKAWS GTSKT DNWS++ KDKAE     VQEV   +NGW SAGGW KN
Sbjct: 1469 VEIASKSGGWDDKAWSRGTSKTEDNWSSRAKDKAEPWLAHVQEVSPNSNGWGSAGGWGKN 1528

Query: 1501 SGDADQSEACRNDGQASMDLETVADRWGSMATQRK-DSKDNFPSKAVEHGDSPLINHSWN 1560
            +GD D+SEA RNDGQASMDLE V+DRW     QR  DSKDNF SK VEHGDS  INHSW+
Sbjct: 1529 AGDGDESEAGRNDGQASMDLEKVSDRWDGRDVQRTGDSKDNFQSKVVEHGDSVAINHSWD 1588

Query: 1561 QHKSSEVFRGESGNDFWGQRKSQDVIKPS--------QGWGSQVKSNEGSSQNTQVERLW 1620
            Q K  EV +GE GND WGQ+KS +V KPS         GWGS+++ NEG +         
Sbjct: 1589 QQKPPEVSQGEYGNDAWGQQKSWEVKKPSHVNNESNRHGWGSRIELNEGPN--------- 1648

Query: 1621 SSQNESDQVASEHKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQND------------ 1680
               +E DQV     ++DS GWDSQ++++KPW+KQKS EASQSW SQ D            
Sbjct: 1649 ---HECDQV-----TNDSGGWDSQKQMDKPWEKQKSTEASQSWGSQKDSQSWGSQKDSQS 1708

Query: 1681 --------------------------------------------SMGSWGQLQRESEEFS 1740
                                                        S GSWGQLQR  +EFS
Sbjct: 1709 WGSQKDSQSWGSQKDSQSWGTQKDSQSWGSQKDSQSWGSLKDSQSQGSWGQLQRTPKEFS 1768

Query: 1741 QGSQDDSNKQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSHSWGSHKES 1800
            Q SQDDSNK F   QK PE S GW   K       SHGWGSH +SS+  +SH W +    
Sbjct: 1769 QESQDDSNKHFDN-QKPPETSSGWEQQKSPE---VSHGWGSHIDSSDSTSSHGWDN---- 1828

Query: 1801 SELTTSHAWEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMF 1860
                      KKNQGSK WG NVGEWKNRKNRPPKSPG+ +DDA LR +YTASGQRLDMF
Sbjct: 1829 ----------KKNQGSKSWGGNVGEWKNRKNRPPKSPGMTSDDANLRGLYTASGQRLDMF 1888

Query: 1861 TTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGI 1920
            TTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQ+VFNFHPDKAVKMGAGI
Sbjct: 1889 TTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQSVFNFHPDKAVKMGAGI 1948

Query: 1921 DHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRS 1948
            DHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNF+KGKYPD+AE FVAKYFRKPRS
Sbjct: 1949 DHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNFIKGKYPDMAEMFVAKYFRKPRS 1982

BLAST of MS018728 vs. ExPASy Swiss-Prot
Match: Q5D869 (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 1798.1 bits (4656), Expect = 0.0e+00
Identity = 1005/1964 (51.17%), Postives = 1286/1964 (65.48%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CI +IS+  I H SQL+N FLGLP+EFGKCESCG +EP KCEGHFGYI+LP+PI+HP H+
Sbjct: 28   CIQSISESAINHPSQLTNAFLGLPLEFGKCESCGATEPDKCEGHFGYIQLPVPIYHPAHV 87

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
             ELK+MLSLLCLKCLK+KK K  S   G A+RLL  CCE+ASQ+SI++ + +DGASYL+L
Sbjct: 88   NELKQMLSLLCLKCLKIKKAKGTSG--GLADRLLGVCCEEASQISIKD-RASDGASYLEL 147

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            K+PSR+ L+ G W+FLERYG+RYG + TR LL  EVKE+L++IP E+RKKL  KG+ PQ+
Sbjct: 148  KLPSRSRLQPGCWNFLERYGYRYGSDYTRPLLAREVKEILRRIPEESRKKLTAKGHIPQE 207

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYIL+YLPVPPNCLSVPE SDG + MS DP+   LK +LK+V  IK SRSG  NFESH+ 
Sbjct: 208  GYILEYLPVPPNCLSVPEASDGFSTMSVDPSRIELKDVLKKVIAIKSSRSGETNFESHKA 267

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EA+++   VD YLQVRGT KA+R ID RYGV+K  +  S+KAW EKMRTLFIRKGSGFSS
Sbjct: 268  EASEMFRVVDTYLQVRGTAKAARNIDMRYGVSKISDSSSSKAWTEKMRTLFIRKGSGFSS 327

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAY+ VNE+G+P E+AQRITFEERVSVHN  YLQ+LVD KLCL+Y  GS+ YSL
Sbjct: 328  RSVITGDAYRHVNEVGIPIEIAQRITFEERVSVHNRGYLQKLVDDKLCLSYTQGSTTYSL 387

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            R+GS GHT LKPGQ+VHRR+MDGD+VFINRPPTTHKHSLQALRVY+H+D+TVKINPL+C 
Sbjct: 388  RDGSKGHTELKPGQVVHRRVMDGDVVFINRPPTTHKHSLQALRVYVHEDNTVKINPLMCS 447

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PLSADFDGDC+HLFYPQS++AKAEV+ LFSVEKQLLSSH+G L LQ+G+DSLLSL++M  
Sbjct: 448  PLSADFDGDCVHLFYPQSLSAKAEVMELFSVEKQLLSSHTGQLILQMGSDSLLSLRVMLE 507

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
              FL KA AQQLAM+ S SLP PA+  +    P WT  QILQ   P    C GD +L+  
Sbjct: 508  RVFLDKATAQQLAMYGSLSLPPPALRKSSKSGPAWTVFQILQLAFPERLSCKGDRFLVDG 567

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            SD LKFDF  DAM S+INEIVTSIF + GP+E L FFDSLQPLLME +F+EGFS+ L+D 
Sbjct: 568  SDLLKFDFGVDAMGSIINEIVTSIFLEKGPKETLGFFDSLQPLLMESLFAEGFSLSLEDL 627

Query: 601  SMPMALLQALQK-NIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGK 660
            SM  A +  +    I+ ISP++ +LR ++ +  ELQLEN I  VK    NF+LK  S+  
Sbjct: 628  SMSRADMDVIHNLIIREISPMVSRLRLSYRD--ELQLENSIHKVKEVAANFMLKSYSIRN 687

Query: 661  LFDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFG 720
            L D KS+SAI K+VQQ GFLGLQLSDK KFY+KTL+ED+A     +Y   +I   S +FG
Sbjct: 688  LIDIKSNSAITKLVQQTGFLGLQLSDKKKFYTKTLVEDMAIFCKRKY--GRIS-SSGDFG 747

Query: 721  LVKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRN 780
            +VKGCFFHGLDPYEEM HSI+ REV+VRSSRGL EPGTLFKNLMA+LRD+VI  DGTVRN
Sbjct: 748  IVKGCFFHGLDPYEEMAHSIAAREVIVRSSRGLAEPGTLFKNLMAVLRDIVITNDGTVRN 807

Query: 781  VCSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMM 840
             CSNS+IQ +YGV +       LF  GEPVGVLAATAMSNPAYKAVLDS+P+S SSW++M
Sbjct: 808  TCSNSVIQFKYGVDS-ERGHQGLFEAGEPVGVLAATAMSNPAYKAVLDSSPNSNSSWELM 867

Query: 841  KEILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIE 900
            KE+LLCKV+F+N   DRRVILYLN C CG++ C ENAA  V++ L KV+LKD  V+F++E
Sbjct: 868  KEVLLCKVNFQNTTNDRRVILYLNECHCGKRFCQENAACTVRNKLNKVSLKDTAVEFLVE 927

Query: 901  YNRQLTLS---GFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHA 960
            Y +Q T+S   G    L GH+HLNK LL++  I+M D+ ++CE+ I+S  +KKKKK    
Sbjct: 928  YRKQPTISEIFGIDSCLHGHIHLNKTLLQDWNISMQDIHQKCEDVINSLGQKKKKKATDD 987

Query: 961  LR---FSFSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIK 1020
             +    S SE CSF    G   +DMPCL F +   D  LERT  +  + V+P+L E +IK
Sbjct: 988  FKRTSLSVSECCSFRDPCGSKGSDMPCLTFSYNATDPDLERTLDVLCNTVYPVLLEIVIK 1047

Query: 1021 GDPRISAANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPV 1080
            GD RI +AN+IW S D T+W RN    + GE  LD+ +EKSAVKQ+GDAWR V+D CL V
Sbjct: 1048 GDSRICSANIIWNSSDMTTWIRNRHASRRGEWVLDVTVEKSAVKQSGDAWRVVIDSCLSV 1107

Query: 1081 IHLIDTRRSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCT 1140
            +HLIDT+RSIPY++KQVQELLG+SCAF+Q VQRL+ SV MVSKGVL +H+ILLAN+MTC+
Sbjct: 1108 LHLIDTKRSIPYSVKQVQELLGLSCAFEQAVQRLSASVRMVSKGVLKEHIILLANNMTCS 1167

Query: 1141 GNMIGFNSGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKH 1200
            G M+GFNSGGYKAL+R+LNI+ PFTEATL  PR+CFE+AAEKCH DSLS++V SCSWGK 
Sbjct: 1168 GTMLGFNSGGYKALTRSLNIKAPFTEATLIAPRKCFEKAAEKCHTDSLSTVVGSCSWGKR 1227

Query: 1201 VAVGTGSRFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDE 1260
            V VGTGS+F++LW+QKE G    +  DVY+FL MV S  + +   +  G ++     E+E
Sbjct: 1228 VDVGTGSQFELLWNQKETGLDDKEETDVYSFLQMVISTTNADAFVSSPGFDV----TEEE 1287

Query: 1261 YGELTLSPEPFSTSEKPVFEDSAEFENCLD-NYPGESKWEKAPPSGAGSTGGGQWENNEN 1320
              E   SPE  S   +P FEDSA+F+N  D   P  + WEK+     G +GG +W  +++
Sbjct: 1288 MAEWAESPERDSALGEPKFEDSADFQNLHDEGKPSGANWEKSSSWDNGCSGGSEWGVSKS 1347

Query: 1321 T-----------KATNSSNDHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATN 1380
            T           K TN   +  WS W         T K  + +SKS  DS  +WG K   
Sbjct: 1348 TGGEANPESNWEKTTNVEKEDAWSSWN--------TRKDAQESSKS--DSGGAWGIK--- 1407

Query: 1381 TTTNDNDWSNSATKEVEP---DSFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEP-- 1440
              T D D   +   E  P   DS     N P S  W   +    K+ D  N+  E+ P  
Sbjct: 1408 --TKDADADTTPNWETSPAPKDSIVPENNEPTSDVWGHKSV-SDKSWDKKNWGTESAPAA 1467

Query: 1441 ---EKANVWSGWQNDKAETQDAFNKKINSRSCGSEDK-------------AWSTGTSKTY 1500
                 A VW       +ET+       ++ + GS DK              W+  +S+T 
Sbjct: 1468 WGSTDAAVWGSSDKKNSETES------DAAAWGSRDKNNSDVGSGAGVLGPWNKKSSETE 1527

Query: 1501 DN---WSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNSGDADQSEACRNDGQASMDL 1560
             N   W +  K K+ +         +  N WD     +KN     +  A  + G+ + + 
Sbjct: 1528 SNGATWGSSDKTKSGA---------AAWNSWD-----KKNIETDSEPAAWGSQGKKNSET 1587

Query: 1561 ETVADRWGSMATQRKDSKDNFPSKAVEHGDSPLINHSWNQHKSSEVFRGESGNDFWGQRK 1620
            E+    WG+   ++ +++   P  A                K+SE   G +    W ++K
Sbjct: 1588 ESGPAAWGAWDKKKSETE---PGPA---------GWGMGDKKNSETELGPAAMGNWDKKK 1647

Query: 1621 SQDVIKPSQGWGSQVKSNEGSSQNTQVERLWSSQNESDQVASEHKSSDSRGWDSQEKLNK 1680
            S     P+  WGS   +  GSS         +S+ ESD  A          W S+ K   
Sbjct: 1648 SDTKSGPA-AWGSTDAAAWGSSDKN------NSETESDAAA----------WGSRNK--- 1707

Query: 1681 PWDKQKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSNKQFSQVQKSPEVSHGWG 1740
               K   +E+         + GSWGQ    +E+    ++DD N               W 
Sbjct: 1708 ---KTSEIESGAG------AWGSWGQPSPTAED-KDTNEDDRNP--------------WV 1767

Query: 1741 SHKESSELTTSHGWGSHKESSELATSHSWGSHKESSELTTSHAWEKKNQGSKGWGANVG- 1800
            S KE+            K+  E +    WG+              KK   S GW    G 
Sbjct: 1768 SLKETK--------SREKDDKERS---QWGNP------------AKKFPSSGGWSNGGGA 1827

Query: 1801 EWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDILADIEPIMQSIRKIMHQ 1860
            +WK  +N  P+ P     +  L  ++TA+ QRLD FT+EEQ++L+D+EP+M+++RKIMH 
Sbjct: 1828 DWKGNRNHTPRPP---RSEDNLAPMFTATRQRLDSFTSEEQELLSDVEPVMRTLRKIMHP 1860

Query: 1861 SGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRHSSFQESRCFYVVSTDG 1920
            S Y DGDP+S +D++F+L+ + NFHP K  K+G+G+D   V +H+ F +SRCF+VVSTDG
Sbjct: 1888 SAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFITVDKHTIFSDSRCFFVVSTDG 1860

BLAST of MS018728 vs. ExPASy Swiss-Prot
Match: Q9LQ02 (DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD1 PE=1 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 1.2e-102
Identity = 343/1312 (26.14%), Postives = 575/1312 (43.83%), Query Frame = 0

Query: 14   SQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHITELKKMLSLLCLK 73
            +Q+++  LGLP     C +CG+ +   CEGHFG I     I +P  + E+  +L+ +C  
Sbjct: 40   NQVTDSRLGLPNPDSVCRTCGSKDRKVCEGHFGVINFAYSIINPYFLKEVAALLNKICPG 99

Query: 74   CLKMKKNKFP------------SKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQLK 133
            C  ++K +F             + N G+         ++  + S   + + +  S ++LK
Sbjct: 100  CKYIRKKQFQITEDQPERCRYCTLNTGYPLMKFRVTTKEVFRRS-GIVVEVNEESLMKLK 159

Query: 134  VPSRTPLREGFWDFLERYGFRYGDNL---TRTLLPCEVKEMLKKIPNEARKKLAGKGYYP 193
                  L   +W FL +        L    R +   +V  +L  I     ++L  K    
Sbjct: 160  KRGVLTLPPDYWSFLPQDSNIDESCLKPTRRIITHAQVYALLLGID----QRLIKKDIPM 219

Query: 194  QDGYILQYLPVPPNCLSVPEI---SDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNF 253
             +   L   PV PN   V EI    +G  ++  D    + KK++               F
Sbjct: 220  FNSLGLTSFPVTPNGYRVTEIVHQFNGARLI-FDERTRIYKKLV--------------GF 279

Query: 254  ESHEVEANDLQLAVDQYLQV-RGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRK 313
            E + +E +   +   QY ++   TV +S+     Y   ++ +D      L  M+ + + K
Sbjct: 280  EGNTLELSSRVMECMQYSRLFSETVSSSKDSANPY---QKKSDTPKLCGLRFMKDVLLGK 339

Query: 314  GSGFSSRSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHN-----INYLQELVDKKLCL 373
             S  + R+V+ GD    +NEIG+P  +A+R+   E ++  N      +++  L+D K  +
Sbjct: 340  RSDHTFRTVVVGDPSLKLNEIGIPESIAKRLQVSEHLNQCNKERLVTSFVPTLLDNKE-M 399

Query: 374  TYRDGSSAYSLREGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRV-YLHD 433
              R G    +++        L+ G  + R +MDGD V +NRPP+ H+HSL A+ V  L  
Sbjct: 400  HVRRGDRLVAIQVND-----LQTGDKIFRSLMDGDTVLMNRPPSIHQHSLIAMTVRILPT 459

Query: 434  DHTVKINPLICGPLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLG 493
               V +NP+ C P   DFDGDC+H + PQSI AK E+  L +++KQL++  +G   L LG
Sbjct: 460  TSVVSLNPICCLPFRGDFDGDCLHGYVPQSIQAKVELDELVALDKQLINRQNGRNLLSLG 519

Query: 494  TDSLLS--LKMMFRTYFLGKAAAQQLAMFVSSSLPSPAILGA--RSDSPHWTALQILQTV 553
             DSL +  L  + +  +L +A  QQL M+    LP PAI+ A   S  P WT +Q+   +
Sbjct: 520  QDSLTAAYLVNVEKNCYLNRAQMQQLQMYCPFQLPPPAIIKASPSSTEPQWTGMQLFGML 579

Query: 554  LPACFDCHG--DSYLIKNSDFLKFD----FDRDAMPSLINEIVTSIFFQNGPEEVLRFFD 613
             P  FD     ++ ++ N + L F     + RD   + I  ++     ++   +VL    
Sbjct: 580  FPPGFDYTYPLNNVVVSNGELLSFSEGSAWLRDGEGNFIERLL-----KHDKGKVLDIIY 639

Query: 614  SLQPLLMEHIFSEGFSVGLDDYSMPMALLQALQKNIQVISPLLYQLR------------- 673
            S Q +L + +   G SV L D    + L   LQ    +   + Y LR             
Sbjct: 640  SAQEMLSQWLLMRGLSVSLAD----LYLSSDLQSRKNLTEEISYGLREAEQVCNKQQLMV 699

Query: 674  --------------------------------STFNELVELQLENHIRSVKVPFTNFILK 733
                                            +T +EL     ++  R V+     +  +
Sbjct: 700  ESWRDFLAVNGEDKEEDSVSDLARFCYERQKSATLSELAVSAFKDAYRDVQALAYRYGDQ 759

Query: 734  LSSLGKLFDSKSDSAINKVVQQIGFLGLQLSDKGKFY------SKTLIEDVASLFHNRYV 793
             +S   +  + S   I K+VQ    +GLQ S     +      +     D  S       
Sbjct: 760  SNSFLIMSKAGSKGNIGKLVQHSMCIGLQNSAVSLSFGFPRELTCAAWNDPNSPLRGAKG 819

Query: 794  SDKIDYPS-AEFGLVKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAIL 853
             D     S   +G+++  F  GL+P E  VHS+++R+     +  L  PGTL + LM  +
Sbjct: 820  KDSTTTESYVPYGVIENSFLTGLNPLESFVHSVTSRDSSFSGNADL--PGTLSRRLMFFM 879

Query: 854  RDVVICYDGTVRNVCSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVL 913
            RD+   YDGTVRN   N ++Q  Y     +         GE +G L+A A+S  AY A L
Sbjct: 880  RDIYAAYDGTVRNSFGNQLVQFTYETDGPVED-----ITGEALGSLSACALSEAAYSA-L 939

Query: 914  DSTPS--STSSWDMMKEILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHL 973
            D   S   TS    +K +L C    K    ++ + LYL+     +KH  E  +  +K+HL
Sbjct: 940  DQPISLLETSPLLNLKNVLEC--GSKKGQREQTMSLYLSEYLSKKKHGFEYGSLEIKNHL 999

Query: 974  KKVTLKDATVDFMIEY----NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEE 1033
            +K++  +     MI +    N ++ LS +    V H H+++ +LK  +++   V     E
Sbjct: 1000 EKLSFSEIVSTSMIIFSPSSNTKVPLSPW----VCHFHISEKVLKRKQLSAESVVSSLNE 1059

Query: 1034 TISSFRKKKKKKFAHALRFSFSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFA 1093
               S R ++ K     L    + +CS      +D  D  C+         H         
Sbjct: 1060 QYKS-RNRELKLDIVDLDIQNTNHCSSDDQAMKD--DNVCITVTVVEASKHSVLELDAIR 1119

Query: 1094 DIVFPLLSETIIKGDPRISAANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNG 1153
             ++ P L ++ +KGD  I   N++W   D     +       GEL L + +     K+N 
Sbjct: 1120 LVLIPFLLDSPVKGDQGIKKVNILW--TDRPKAPKRNGNHLAGELYLKVTMYGDRGKRN- 1179

Query: 1154 DAWRNVMDCCLPVIHLIDTRRSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLG 1213
              W  +++ CLP++ +ID  RS P  I+Q   + GI       V  L  +VS   K +L 
Sbjct: 1180 -CWTALLETCLPIMDMIDWGRSHPDNIRQCCSVYGIDAGRSIFVANLESAVSDTGKEILR 1239

Query: 1214 DHLILLANSMTCTGNMIGFNSGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDS 1233
            +HL+L+A+S++ TG  +  N+ G+    +  +   PFT+A   +P +CF +AA++  +D 
Sbjct: 1240 EHLLLVADSLSVTGEFVALNAKGWSKQRQVESTPAPFTQACFSSPSQCFLKAAKEGVRDD 1290

BLAST of MS018728 vs. ExPASy Swiss-Prot
Match: P36594 (DNA-directed RNA polymerase II subunit rpb1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpb1 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 5.9e-57
Identity = 222/843 (26.33%), Postives = 366/843 (43.42%), Query Frame = 0

Query: 16  LSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHITELKKMLSLLCLKCL 75
           L +P LG      KC++CG +    C GHFG+IEL  P+FH   ++++KK+L  +C  C 
Sbjct: 55  LLDPRLGTIDRQFKCQTCGET-MADCPGHFGHIELAKPVFHIGFLSKIKKILECVCWNCG 114

Query: 76  KMK----KNKF-PSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQLKVPSR----- 135
           K+K      KF  ++     +  L++         + +   + G+    L  PS      
Sbjct: 115 KLKIDSSNPKFNDTQRYRDPKNRLNAVWNVCKTKMVCDTGLSAGSDNFDLSNPSANMGHG 174

Query: 136 -------TPLREG--FWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLA-GKG 195
                  T  ++G   W   +R          R L P EV  +   I +E    L   + 
Sbjct: 175 GCGAAQPTIRKDGLRLWGSWKRGKDESDLPEKRLLSPLEVHTIFTHISSEDLAHLGLNEQ 234

Query: 196 YYPQDGYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILK-QVEIIKGSRSGAPN 255
           Y   D  I+  LPVPP  +  P IS   T    D     L  I+K    + +  + GAP 
Sbjct: 235 YARPDWMIITVLPVPPPSVR-PSISVDGTSRGEDDLTHKLSDIIKANANVRRCEQEGAPA 294

Query: 256 FESHEVEANDLQLAVDQYL--QVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFI 315
               E E   LQ  V  Y+  ++ G  +A +    + G   +      K    ++R   +
Sbjct: 295 HIVSEYE-QLLQFHVATYMDNEIAGQPQALQ----KSGRPLKSIRARLKGKEGRLRGNLM 354

Query: 316 RKGSGFSSRSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELV----DKKLC 375
            K   FS+R+VITGD    ++E+GVP  +A+ +T+ E V+ +NI  LQELV    D+   
Sbjct: 355 GKRVDFSARTVITGDPNLSLDELGVPRSIAKTLTYPETVTPYNIYQLQELVRNGPDEHPG 414

Query: 376 LTY--RDGSSAYSLR-EGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVY 435
             Y  RD      LR     G   L+ G  V R I DGD+V  NR P+ HK S+   R+ 
Sbjct: 415 AKYIIRDTGERIDLRYHKRAGDIPLRYGWRVERHIRDGDVVIFNRQPSLHKMSMMGHRIR 474

Query: 436 LHDDHTVKINPLICGPLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNL 495
           +    T ++N  +  P +ADFDGD +++  PQS   +AE+  +  V KQ++S  S    +
Sbjct: 475 VMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPVM 534

Query: 496 QLGTDSLLSL-KMMFRTYFLGKAAAQQLAMFV---SSSLPSPAILGARSDSPHWTALQIL 555
            +  D+L  + K   R  FL + A   + ++V      LP P IL        WT  QIL
Sbjct: 535 GIVQDTLAGVRKFSLRDNFLTRNAVMNIMLWVPDWDGILPPPVIL---KPKVLWTGKQIL 594

Query: 556 QTVLPACFDCHGD------------SYLIKNSDFLKFDFDRDAMPSLINEIVTSIFFQNG 615
             ++P   +   D              LI+N + +    D+  + +    +V +I+ + G
Sbjct: 595 SLIIPKGINLIRDDDKQSLSNPTDSGMLIENGEIIYGVVDKKTVGASQGGLVHTIWKEKG 654

Query: 616 PEEVLRFFDSLQPLLMEHIFSEGFSVGLDDYSMPMALLQALQKNIQVISPLLYQLRSTFN 675
           PE    FF+ +Q ++   +   GFS+G+ D       ++ + + ++       + R    
Sbjct: 655 PEICKGFFNGIQRVVNYWLLHNGFSIGIGDTIADADTMKEVTRTVK-------EARRQVA 714

Query: 676 ELVELQLENHIRSVKVPFTNFILKLS---SLGKLFDSKSDSA----------INKVVQQI 735
           E ++    N ++    P     L+ S    + ++ +   D+A           N V Q +
Sbjct: 715 ECIQDAQHNRLK----PEPGMTLRESFEAKVSRILNQARDNAGRSAEHSLKDSNNVKQMV 774

Query: 736 --GFLG--LQLSDKGKFYSKTLIEDVASLFHNRYVS----DKIDYPSAEFGLVKGCFFHG 792
             G  G  + +S       + ++E     F  +Y +     K D      G ++  +  G
Sbjct: 775 AAGSKGSFINISQMSACVGQQIVEGKRIPFGFKYRTLPHFPKDDDSPESRGFIENSYLRG 834

BLAST of MS018728 vs. ExPASy Swiss-Prot
Match: P11414 (DNA-directed RNA polymerase II subunit RPB1 OS=Cricetulus griseus OX=10029 GN=POLR2A PE=1 SV=2)

HSP 1 Score: 209.9 bits (533), Expect = 2.6e-52
Identity = 218/867 (25.14%), Postives = 359/867 (41.41%), Query Frame = 0

Query: 16  LSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHITELKKMLSLLCLKCL 75
           L +P  G+    G+C++C      +C GHFG+IEL  P+FH   + +  K+L  +C  C 
Sbjct: 57  LMDPRQGVIERTGRCQTC-AGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCS 116

Query: 76  KM-------KKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQLKVPSRTPL 135
           K+       K     +K+ G  ++ L+   +     +I      +G   +  K     P 
Sbjct: 117 KLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNI-----CEGGEEMDNKFGVEQP- 176

Query: 136 REGFWDFLERYGF----RYGDNLTRT---------------------LLPCEVKEMLKKI 195
            EG  D  +  G     RY   + R+                     L P  V E+ K+I
Sbjct: 177 -EGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRI 236

Query: 196 PNEARKKLAGKGYYPQ-DGYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQV 255
            +E    L  +  Y + +  I+  LPVPP  +    +  G      D    +  K+   V
Sbjct: 237 SDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDD----LTHKLADIV 296

Query: 256 EIIKGSRSGAPNFESHEVEANDLQL-------AVDQYLQ--VRGTVKASRGIDARYGVNK 315
           +I    R    N  +  V A D++L        VD  L    R   K+ R +       K
Sbjct: 297 KINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPL-------K 356

Query: 316 ELNDPSTKAWLEKMRTLFIRKGSGFSSRSVITGDAYKLVNEIGVPFEVAQRITFEERVSV 375
            L     K    ++R   + K   FS+R+VIT D    ++++GVP  +A  +TF E V+ 
Sbjct: 357 SLKQ-RLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTP 416

Query: 376 HNINYLQELVDK------KLCLTYRDGSSAYSLR-EGSMGHTYLKPGQIVHRRIMDGDIV 435
            NI+ LQELV +            RD      LR        +L+ G  V R + DGDIV
Sbjct: 417 FNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIV 476

Query: 436 FINRPPTTHKHSLQALRVYLHDDHTVKINPLICGPLSADFDGDCIHLFYPQSIAAKAEVL 495
             NR PT HK S+   RV +    T ++N  +  P +ADFDGD ++L  PQS+  +AE+ 
Sbjct: 477 IFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQ 536

Query: 496 GLFSVEKQLLSSHSGNLNLQLGTDSLLSL-KMMFRTYFLGKAAAQQLAMFVSS---SLPS 555
            L  V + +++  S    + +  D+L ++ K   R  FL +     L MF+S+    +P 
Sbjct: 537 ELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQ 596

Query: 556 PAILGARSDSPHWTALQILQTVLPACFDC------HGD---------------SYLIKNS 615
           PAIL  R   P WT  QI   ++P   +C      H D                 +++N 
Sbjct: 597 PAILKPR---PLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENG 656

Query: 616 DFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDYS 675
           + +     + ++ +    +V   + + G +    F+ ++Q ++   +  EG ++G+ D  
Sbjct: 657 ELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSI 716

Query: 676 MPMALLQALQKNI-QVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 735
                 Q +Q  I +    ++  +    N  +E    N +R     F N + ++  L   
Sbjct: 717 ADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQT---FENQVNRI--LNDA 776

Query: 736 FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSD----------- 792
            D    SA   + +   F  + +S  G   SK  I  V ++   + V             
Sbjct: 777 RDKTGSSAQKSLSEYNNFKSMVVS--GAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRT 836

BLAST of MS018728 vs. ExPASy Swiss-Prot
Match: P24928 (DNA-directed RNA polymerase II subunit RPB1 OS=Homo sapiens OX=9606 GN=POLR2A PE=1 SV=2)

HSP 1 Score: 209.9 bits (533), Expect = 2.6e-52
Identity = 218/867 (25.14%), Postives = 359/867 (41.41%), Query Frame = 0

Query: 16  LSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHITELKKMLSLLCLKCL 75
           L +P  G+    G+C++C      +C GHFG+IEL  P+FH   + +  K+L  +C  C 
Sbjct: 57  LMDPRQGVIERTGRCQTC-AGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCS 116

Query: 76  KM-------KKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQLKVPSRTPL 135
           K+       K     +K+ G  ++ L+   +     +I      +G   +  K     P 
Sbjct: 117 KLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNI-----CEGGEEMDNKFGVEQP- 176

Query: 136 REGFWDFLERYGF----RYGDNLTRT---------------------LLPCEVKEMLKKI 195
            EG  D  +  G     RY   + R+                     L P  V E+ K+I
Sbjct: 177 -EGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRI 236

Query: 196 PNEARKKLAGKGYYPQ-DGYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQV 255
            +E    L  +  Y + +  I+  LPVPP  +    +  G      D    +  K+   V
Sbjct: 237 SDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDD----LTHKLADIV 296

Query: 256 EIIKGSRSGAPNFESHEVEANDLQL-------AVDQYLQ--VRGTVKASRGIDARYGVNK 315
           +I    R    N  +  V A D++L        VD  L    R   K+ R +       K
Sbjct: 297 KINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPL-------K 356

Query: 316 ELNDPSTKAWLEKMRTLFIRKGSGFSSRSVITGDAYKLVNEIGVPFEVAQRITFEERVSV 375
            L     K    ++R   + K   FS+R+VIT D    ++++GVP  +A  +TF E V+ 
Sbjct: 357 SLKQ-RLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTP 416

Query: 376 HNINYLQELVDK------KLCLTYRDGSSAYSLR-EGSMGHTYLKPGQIVHRRIMDGDIV 435
            NI+ LQELV +            RD      LR        +L+ G  V R + DGDIV
Sbjct: 417 FNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIV 476

Query: 436 FINRPPTTHKHSLQALRVYLHDDHTVKINPLICGPLSADFDGDCIHLFYPQSIAAKAEVL 495
             NR PT HK S+   RV +    T ++N  +  P +ADFDGD ++L  PQS+  +AE+ 
Sbjct: 477 IFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQ 536

Query: 496 GLFSVEKQLLSSHSGNLNLQLGTDSLLSL-KMMFRTYFLGKAAAQQLAMFVSS---SLPS 555
            L  V + +++  S    + +  D+L ++ K   R  FL +     L MF+S+    +P 
Sbjct: 537 ELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQ 596

Query: 556 PAILGARSDSPHWTALQILQTVLPACFDC------HGD---------------SYLIKNS 615
           PAIL  R   P WT  QI   ++P   +C      H D                 +++N 
Sbjct: 597 PAILKPR---PLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENG 656

Query: 616 DFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDYS 675
           + +     + ++ +    +V   + + G +    F+ ++Q ++   +  EG ++G+ D  
Sbjct: 657 ELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSI 716

Query: 676 MPMALLQALQKNI-QVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 735
                 Q +Q  I +    ++  +    N  +E    N +R     F N + ++  L   
Sbjct: 717 ADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQT---FENQVNRI--LNDA 776

Query: 736 FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSD----------- 792
            D    SA   + +   F  + +S  G   SK  I  V ++   + V             
Sbjct: 777 RDKTGSSAQKSLSEYNNFKSMVVS--GAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRT 836

BLAST of MS018728 vs. ExPASy TrEMBL
Match: A0A6J1CY08 (DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC111015618 PE=3 SV=1)

HSP 1 Score: 3900.9 bits (10115), Expect = 0.0e+00
Identity = 1938/1947 (99.54%), Postives = 1939/1947 (99.59%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL
Sbjct: 89   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD
Sbjct: 149  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV
Sbjct: 209  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR
Sbjct: 449  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
            TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPA FDCHGDSYLIKN
Sbjct: 509  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPAYFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY
Sbjct: 569  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL
Sbjct: 689  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK
Sbjct: 809  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY
Sbjct: 869  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS
Sbjct: 929  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
            FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA
Sbjct: 989  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR
Sbjct: 1049 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEE TSACLGEEI+DLMVEDEY ELTLS
Sbjct: 1229 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEEFTSACLGEEIEDLMVEDEYRELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTG GQWENNENTKATNSSN
Sbjct: 1289 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGSGQWENNENTKATNSSN 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
            DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD
Sbjct: 1349 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFNKKI 1440
            SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAF KKI
Sbjct: 1409 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFIKKI 1468

Query: 1441 NSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNSGD 1500
            NSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNSGD
Sbjct: 1469 NSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNSGD 1528

Query: 1501 ADQSEACRNDGQASMDLETVADRWGSMATQRKDSKDNFPSKAVEHGDSPLINHSWNQHKS 1560
            ADQSEACRND QASMDLETVADRWGS ATQRKDSKDNFPSKAVEHGDSPLINHSWNQHKS
Sbjct: 1529 ADQSEACRNDDQASMDLETVADRWGSRATQRKDSKDNFPSKAVEHGDSPLINHSWNQHKS 1588

Query: 1561 SEVFRGESGNDFWGQRKSQDVIKPSQGWGSQVKSNEGSSQNTQVERLWSSQNESDQVASE 1620
            SEVFRGESGNDFWGQRKSQDVIKPSQGWGSQVKSNEGSSQNTQVERLWSSQNESDQVASE
Sbjct: 1589 SEVFRGESGNDFWGQRKSQDVIKPSQGWGSQVKSNEGSSQNTQVERLWSSQNESDQVASE 1648

Query: 1621 HKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSN 1680
            HKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSN
Sbjct: 1649 HKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSN 1708

Query: 1681 KQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSHSWGSHKESSELTTSHA 1740
            KQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSH WGSHKESSELTTSHA
Sbjct: 1709 KQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSHGWGSHKESSELTTSHA 1768

Query: 1741 WEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDIL 1800
            WEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDIL
Sbjct: 1769 WEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDIL 1828

Query: 1801 ADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRH 1860
            ADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRH
Sbjct: 1829 ADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRH 1888

Query: 1861 SSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRSGKPRDRNS 1920
            SSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRSGKPRDRNS
Sbjct: 1889 SSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRSGKPRDRNS 1948

Query: 1921 ASEENENKNVGKELTPIPEETENGNQQ 1948
            ASEENENKNVGKELTPIPEETENGNQQ
Sbjct: 1949 ASEENENKNVGKELTPIPEETENGNQQ 1975

BLAST of MS018728 vs. ExPASy TrEMBL
Match: A0A0A0KN85 (DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_5G435050 PE=3 SV=1)

HSP 1 Score: 3345.8 bits (8674), Expect = 0.0e+00
Identity = 1683/1971 (85.39%), Postives = 1783/1971 (90.46%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPI+HPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIYHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TELKKMLSLLCLKCLKMKK KFPSKNIGFAERLLSSCCEDASQV+IRE KKADGASYLQL
Sbjct: 89   TELKKMLSLLCLKCLKMKKTKFPSKNIGFAERLLSSCCEDASQVTIREAKKADGASYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRT L+E FWDFLERYGFRYGDN TRTLLPCEVKEMLKKIPNE RKKLAG+GYYPQD
Sbjct: 149  KVPSRTSLQERFWDFLERYGFRYGDNFTRTLLPCEVKEMLKKIPNETRKKLAGRGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYILQYLPVPPNCLSVPEISDGVT+MSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV
Sbjct: 209  GYILQYLPVPPNCLSVPEISDGVTVMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQLAVDQYLQVRGTVKASRGIDAR+GVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQLAVDQYLQVRGTVKASRGIDARFGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNI YLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNIRYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDH VKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHVVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQL  DSLLSLKMMFR
Sbjct: 449  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLANDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
             YFLGKAAAQQLAMFVSS LP PA+LG RS S HWTALQILQTVLPA FDCHGDSYLIKN
Sbjct: 509  KYFLGKAAAQQLAMFVSSYLPPPALLGVRSGSLHWTALQILQTVLPASFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            S+FLKFDFDRDAMPSLINEI+TSIFFQ GPEEVL+FFDSLQPLLMEHIFSEGFSVGLDDY
Sbjct: 569  SNFLKFDFDRDAMPSLINEILTSIFFQKGPEEVLKFFDSLQPLLMEHIFSEGFSVGLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMA LQALQKNIQV+SPLLYQLRSTFNELVELQLENH+RSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMAFLQALQKNIQVLSPLLYQLRSTFNELVELQLENHLRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKS+SAINKVVQQIGFLGLQLSDKG+FYSK+LIEDVASLFHNRY SDKIDYPSAEFGL
Sbjct: 689  FDSKSESAINKVVQQIGFLGLQLSDKGRFYSKSLIEDVASLFHNRYSSDKIDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYG+KAGMM+P++LFPPGEPVGVLAATAMS PAYKAVLDSTPSS SSWDMMK
Sbjct: 809  CSNSIIQLEYGMKAGMMQPYSLFPPGEPVGVLAATAMSTPAYKAVLDSTPSSNSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKVSFKNEPIDRRVILYLNNCACGRK+CNENAAY+VKSHLKKVTLKDA +DFMIEY
Sbjct: 869  EILLCKVSFKNEPIDRRVILYLNNCACGRKYCNENAAYVVKSHLKKVTLKDAAMDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQ T SG GPGLVGHVHLN+MLLKEL I+M +V RRC+ET+SSF KKKKKK AHALRFS
Sbjct: 929  NRQPTPSGLGPGLVGHVHLNRMLLKELNIDMTEVLRRCQETMSSF-KKKKKKIAHALRFS 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
             SE+C+FHQ NGE+S DMPCLIFWH+TRD HLERTAHI ADIVFPLLSETIIKGDPRI +
Sbjct: 989  ISEHCAFHQWNGEESIDMPCLIFWHQTRDVHLERTAHILADIVFPLLSETIIKGDPRIKS 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            A+VIWISPDSTSWQ+NPSRWQDGELALD+CLEKSAVKQNGDAWRNV+DCCLPV+HLIDTR
Sbjct: 1049 ASVIWISPDSTSWQKNPSRWQDGELALDVCLEKSAVKQNGDAWRNVLDCCLPVLHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RS+PYAIKQVQELLGISCAFDQ +QRL+KSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSVPYAIKQVQELLGISCAFDQMIQRLSKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPR+CFE+AAEKCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRKCFEKAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            RFDILWDQKELGCKQDDV+DVYNFLHMVRS KSEE TSACLGEEI+D+MVEDEYGELTLS
Sbjct: 1229 RFDILWDQKELGCKQDDVVDVYNFLHMVRSGKSEEPTSACLGEEIEDIMVEDEYGELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEPFSTSEKPVFEDSAEFE+CLDNYPGESKWEKAP  GA STGGGQWE+NEN KATNSS+
Sbjct: 1289 PEPFSTSEKPVFEDSAEFEHCLDNYPGESKWEKAPSLGAVSTGGGQWESNENGKATNSSD 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
             +DWSGWGRK EPDV  T AQENTS S WD+T SWGNKATN ++NDNDWSN +TKEVE D
Sbjct: 1349 GNDWSGWGRKAEPDVTVTNAQENTSNSAWDTTSSWGNKATN-SSNDNDWSNCSTKEVERD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFNKK- 1440
            SF SME TPKSGGWD+A+TWGTK KD D+FK ET P+K++ WSG Q DKAETQDAF+KK 
Sbjct: 1409 SFTSMEKTPKSGGWDSASTWGTKTKD-DSFKRETAPKKSSQWSGLQKDKAETQDAFHKKA 1468

Query: 1441 -INSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNS 1500
             + S+S G EDKAWS GTSKT DNWS+QVKDKAES QVQVQEV SKTNGW S GGW KNS
Sbjct: 1469 EMASKSGGWEDKAWSRGTSKTEDNWSSQVKDKAESFQVQVQEVSSKTNGWGSTGGWTKNS 1528

Query: 1501 GDADQSEACRNDGQASMDLETVADRWGSMATQR--------------KDSKDNFPSKAVE 1560
            G   QSEA  NDGQASMD E V+DRW   ATQ+               DSKD+FPSKAV+
Sbjct: 1529 GGDHQSEAGWNDGQASMDREKVSDRWDRKATQKLESHQTSSWGSPTVGDSKDSFPSKAVD 1588

Query: 1561 HGDSPLINHSWNQHKSSEVFRGESGNDFWGQRKSQDVIKPS--------QGWGSQVKSNE 1620
            H DS ++NHSW++ KS E  +G  GND WGQ+KS+DVIKPS         GWGSQ++SNE
Sbjct: 1589 HSDS-VVNHSWDRQKSPEASQG-FGNDAWGQQKSRDVIKPSLANNESNLSGWGSQIESNE 1648

Query: 1621 GSSQNTQVERLWSSQNESDQVASEHKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQND 1680
            GS                DQV +E KSSD+RGWDSQEK +KPWDKQKSLEASQSW SQND
Sbjct: 1649 GSDHG------------FDQVTNEQKSSDTRGWDSQEKTDKPWDKQKSLEASQSWGSQND 1708

Query: 1681 SMGSWGQLQRESEEFSQGSQDDSNKQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKE 1740
            S+GSWGQ QR SEE S+ SQDDS+ QFSQ+ K PE S GW   K                
Sbjct: 1709 SLGSWGQPQRASEECSRESQDDSSTQFSQL-KPPETSLGWEQQKSPE------------- 1768

Query: 1741 SSELATSHSWGSHKESSELTTSHAWEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDA 1800
                  SH WGS+KESSE T+SH W+KKNQGSKGWG N GEWKNRKNRPPKSPG+ NDDA
Sbjct: 1769 -----VSHGWGSNKESSEQTSSHGWDKKNQGSKGWGGNAGEWKNRKNRPPKSPGMSNDDA 1828

Query: 1801 GLRAIYTASGQRLDMFTTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQN 1860
             LRA+YTASGQRLDMFT+EEQDILADIEPIMQSIRK+MHQSGYNDGDPLSAEDQSF+LQ+
Sbjct: 1829 NLRALYTASGQRLDMFTSEEQDILADIEPIMQSIRKVMHQSGYNDGDPLSAEDQSFVLQS 1888

Query: 1861 VFNFHPDKAVKMGAGIDHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYP 1920
            VFNFHPDKA KMGAGIDHFMVSRHSSFQESRCFYVV+TDGHKEDFSYRKCLDNF+KGKYP
Sbjct: 1889 VFNFHPDKAAKMGAGIDHFMVSRHSSFQESRCFYVVTTDGHKEDFSYRKCLDNFIKGKYP 1948

Query: 1921 DIAEPFVAKYFRKPRSGKPRDRNSASEENENKNVGKELTPIPEETENGNQQ 1948
            D+AE FVAKYFRKPR  + RDRN ASEENENK++G ELTPIPEE +NG+QQ
Sbjct: 1949 DLAEMFVAKYFRKPRPNRNRDRNPASEENENKSIGGELTPIPEEAQNGSQQ 1963

BLAST of MS018728 vs. ExPASy TrEMBL
Match: A0A1S3CPU1 (DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103503449 PE=3 SV=1)

HSP 1 Score: 3343.1 bits (8667), Expect = 0.0e+00
Identity = 1681/1971 (85.29%), Postives = 1777/1971 (90.16%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPI+HPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIYHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TEL+KMLSLLCLKCLKMKK KFPSKNIGFAERLLSSCCEDASQV+IRE KKADGASYLQL
Sbjct: 89   TELRKMLSLLCLKCLKMKKTKFPSKNIGFAERLLSSCCEDASQVTIREAKKADGASYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRT L+E FWDFLERYGFRYGDN TRTLLPCEVKEMLKKIPNE RKKLAG+GYYPQD
Sbjct: 149  KVPSRTSLQERFWDFLERYGFRYGDNFTRTLLPCEVKEMLKKIPNETRKKLAGRGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYILQYLPVPPNCLSVPEISDGVT+MSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV
Sbjct: 209  GYILQYLPVPPNCLSVPEISDGVTVMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQLAVDQYLQVRGTVKASRGIDAR+GVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQLAVDQYLQVRGTVKASRGIDARFGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNI YLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNIRYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDH VKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHVVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
             LSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQL  DSLLSLKMMFR
Sbjct: 449  SLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLANDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
             YFLGKAAAQQLAMFVSS LP PA+LG RS S HWTALQILQTVLPACFDCHGDSYLIKN
Sbjct: 509  KYFLGKAAAQQLAMFVSSYLPPPALLGVRSGSLHWTALQILQTVLPACFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            S+FLKFDFD+DAMPSLINEI+TSIFFQ GPEEVL+FFDSLQPLLMEHIFSEGFSVGLDDY
Sbjct: 569  SNFLKFDFDKDAMPSLINEILTSIFFQKGPEEVLKFFDSLQPLLMEHIFSEGFSVGLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMA LQALQKNIQV+SPLLYQLRSTFNELVELQLENH+RSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMAFLQALQKNIQVLSPLLYQLRSTFNELVELQLENHLRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKS+SAINKVVQQIGFLGLQLSDKG+FYSK+LIEDVASLFHNRY SDKIDYPSAEFGL
Sbjct: 689  FDSKSESAINKVVQQIGFLGLQLSDKGRFYSKSLIEDVASLFHNRYSSDKIDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYG+KAGMM+P++LFPPGEPVGVLAATAMS PAYKAVLDSTPSS SSWDMMK
Sbjct: 809  CSNSIIQLEYGMKAGMMQPYSLFPPGEPVGVLAATAMSTPAYKAVLDSTPSSNSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKVSFKNEPIDRRVILYLNNCACGRK+CNENAAY+VKSHLKKVTLKD  VDFMIEY
Sbjct: 869  EILLCKVSFKNEPIDRRVILYLNNCACGRKYCNENAAYVVKSHLKKVTLKDVAVDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQ T SG GPGLVGHVHLN+MLLKEL INM +V RRC+ET+SSF KKKKKK AHALRF+
Sbjct: 929  NRQPTPSGLGPGLVGHVHLNRMLLKELNINMTEVLRRCQETMSSF-KKKKKKVAHALRFA 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
             SE+C+FHQ NG +S DMPCLIFWHETRD HLERTAHI ADIVFPLLSETIIKGDPRI +
Sbjct: 989  ISEHCAFHQWNGVESIDMPCLIFWHETRDVHLERTAHILADIVFPLLSETIIKGDPRIKS 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            A+VIWISPDSTSWQ+NPSRWQDGELALD+CLEKSA+KQNGDAWRNV+DCCLPV+HLIDTR
Sbjct: 1049 ASVIWISPDSTSWQKNPSRWQDGELALDVCLEKSALKQNGDAWRNVLDCCLPVLHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RS+PYAIKQVQELLGISCAFDQ +QRL+KSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSVPYAIKQVQELLGISCAFDQMIQRLSKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPR+CFE+AAEKCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRKCFEKAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            RFDILWDQKELGCKQDDV+DVYNFLHMVRS KSEE TSACLGEE++D+MVEDEYGELTLS
Sbjct: 1229 RFDILWDQKELGCKQDDVVDVYNFLHMVRSGKSEEPTSACLGEEVEDIMVEDEYGELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEPFSTSEKPVFEDSAEFE+CLDN PGESKWEKAP  GA STGGGQWE+N N KAT SS+
Sbjct: 1289 PEPFSTSEKPVFEDSAEFEHCLDNDPGESKWEKAPSLGAVSTGGGQWESNGNGKATKSSD 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
            D+DWSGWGRK EPDV  T AQENTS S WD+T SWGNKAT  T+NDNDWSN +TKEVE D
Sbjct: 1349 DNDWSGWGRKAEPDVTVTNAQENTSNSAWDTTSSWGNKAT-ITSNDNDWSNCSTKEVERD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEPEKANVWSGWQNDKAETQDAFNKK- 1440
            SF SME TPKSGGWDTA+TWGTK KD D+F GET PEK+N WS  Q DKAETQDAF+KK 
Sbjct: 1409 SFTSMEKTPKSGGWDTASTWGTKTKD-DSFNGETAPEKSNQWSSLQKDKAETQDAFHKKA 1468

Query: 1441 -INSRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNS 1500
             + S+S G EDKAWS GTSKT DNWS QVKDKAES QV VQ+V SKTNGW S GGW KNS
Sbjct: 1469 EMASKSSGWEDKAWSRGTSKTEDNWSGQVKDKAESFQVPVQKVSSKTNGWGSTGGWTKNS 1528

Query: 1501 GDADQSEACRNDGQASMDLETVADRWGSMATQRK--------------DSKDNFPSKAVE 1560
            G   Q+EA  NDGQASMD E  +DRW   ATQ+               DSKD+FPSKAV+
Sbjct: 1529 GGDHQAEAGWNDGQASMDREEASDRWDRKATQKLESHQTSSWGSPTVCDSKDSFPSKAVD 1588

Query: 1561 HGDSPLINHSWNQHKSSEVFRGESGNDFWGQRKSQDVIKPS--------QGWGSQVKSNE 1620
            HGDS ++NHSW++ KS E  +G  GND W Q+KSQDVIKPS         GWGSQ++SNE
Sbjct: 1589 HGDS-VVNHSWDRQKSPEASQG-FGNDAWQQQKSQDVIKPSHANNESNRSGWGSQIESNE 1648

Query: 1621 GSSQNTQVERLWSSQNESDQVASEHKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQND 1680
            GS                DQV SE KSSD+RGWDSQEK++KPWDKQKSLEASQSW SQND
Sbjct: 1649 GSDHG------------FDQVTSEQKSSDTRGWDSQEKMDKPWDKQKSLEASQSWGSQND 1708

Query: 1681 SMGSWGQLQRESEEFSQGSQDDSNKQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKE 1740
            S+GSWGQ QR SEEFS+GSQDDS+ QFSQ+ K PE S GW   K                
Sbjct: 1709 SLGSWGQPQRASEEFSRGSQDDSSTQFSQL-KPPETSLGWEQQKSPE------------- 1768

Query: 1741 SSELATSHSWGSHKESSELTTSHAWEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDA 1800
                  SH WGSHKESSE T+SH W+KKNQGSKGWG N GEWKNRKNRPPKSPG+ +DDA
Sbjct: 1769 -----VSHGWGSHKESSEQTSSHGWDKKNQGSKGWGGNAGEWKNRKNRPPKSPGMSSDDA 1828

Query: 1801 GLRAIYTASGQRLDMFTTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQN 1860
             LRA+YTASGQRLDMFTTEEQDILADIEPIMQSIRK+MHQSGYNDGDPLSAEDQSF+LQ+
Sbjct: 1829 NLRALYTASGQRLDMFTTEEQDILADIEPIMQSIRKVMHQSGYNDGDPLSAEDQSFVLQS 1888

Query: 1861 VFNFHPDKAVKMGAGIDHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYP 1920
            VFNFHPDKA KMGAGIDHFMVSRHSSFQESRCFYVV+TDGHKEDFSYRKCLDNF+KGKYP
Sbjct: 1889 VFNFHPDKAAKMGAGIDHFMVSRHSSFQESRCFYVVTTDGHKEDFSYRKCLDNFIKGKYP 1948

Query: 1921 DIAEPFVAKYFRKPRSGKPRDRNSASEENENKNVGKELTPIPEETENGNQQ 1948
            D+AE FVAKYFRKPR  + RDRN ASEENENK+VG ELTPIPEE +NG+QQ
Sbjct: 1949 DMAEMFVAKYFRKPRPNRNRDRNPASEENENKSVGGELTPIPEEAQNGSQQ 1963

BLAST of MS018728 vs. ExPASy TrEMBL
Match: A0A6J1GP51 (DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC111456230 PE=3 SV=1)

HSP 1 Score: 3229.9 bits (8373), Expect = 0.0e+00
Identity = 1647/2015 (81.74%), Postives = 1747/2015 (86.70%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIE+GKCESCGTSEPGKCEGHFGYIELPIPI+HPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEYGKCESCGTSEPGKCEGHFGYIELPIPIYHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TELKKMLSLLCLKCLKMKKNKFPSKN+GFAERLL SCCEDASQVSIRE KK+DGA+YLQL
Sbjct: 89   TELKKMLSLLCLKCLKMKKNKFPSKNVGFAERLL-SCCEDASQVSIREAKKSDGATYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRT LREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNE RKKLAGKGYYPQD
Sbjct: 149  KVPSRTSLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNETRKKLAGKGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GY+LQYLPVPPNCLSVPEISDGVTIMSSDPAV MLKK+LKQVEIIKGSRSGAPNFE+HEV
Sbjct: 209  GYVLQYLPVPPNCLSVPEISDGVTIMSSDPAVLMLKKVLKQVEIIKGSRSGAPNFEAHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQ+AVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQMAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNI YLQELVDKKLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNIKYLQELVDKKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PL ADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQL  DSLLSLKMMFR
Sbjct: 449  PLGADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLANDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
             YF GKAAAQQLAMFV+SSLP PA+LG RS+S HWTALQILQTVLP+CFDCHGDSYLIKN
Sbjct: 509  KYFFGKAAAQQLAMFVTSSLPPPALLGVRSNSLHWTALQILQTVLPSCFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            SDFLKFDFDRDAMPSLINEIVTSIFFQ GPEEV+RFFDSLQPLLMEH+FSEGFSV LDDY
Sbjct: 569  SDFLKFDFDRDAMPSLINEIVTSIFFQKGPEEVMRFFDSLQPLLMEHVFSEGFSVSLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMA LQALQKNIQVISPLLYQLRS+FNELVELQLENHIRSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMAFLQALQKNIQVISPLLYQLRSSFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKSD+AINKVVQQIGFLGLQLSDKGKFYSKTLI+DVASLFHNRY SDK DYPSAEFGL
Sbjct: 689  FDSKSDAAINKVVQQIGFLGLQLSDKGKFYSKTLIDDVASLFHNRYSSDKNDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYG+KAGMMKP+ LFPPGEPVGVLAATAMS PAYKAVLDSTPSS SSWDMMK
Sbjct: 809  CSNSIIQLEYGIKAGMMKPYGLFPPGEPVGVLAATAMSTPAYKAVLDSTPSSNSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKV FKNEP+DRRVILYLNNC CGRKHCNENAAY+VKSHLKKVTLKD  +DFMIEY
Sbjct: 869  EILLCKVGFKNEPVDRRVILYLNNCDCGRKHCNENAAYVVKSHLKKVTLKDVAMDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQ T S  GPGLVGHVHLN++LL+EL+INMADV RRC+ETISSF KKKKKK A ALRF 
Sbjct: 929  NRQPTPSALGPGLVGHVHLNQVLLEELRINMADVLRRCQETISSF-KKKKKKLAPALRFF 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
             SE+CSFHQ NGE+ TDMPCL FW ETRD HLERT+HI AD+VFPLLSETIIKGDPRIS+
Sbjct: 989  ISEHCSFHQRNGEERTDMPCLTFWLETRDVHLERTSHILADVVFPLLSETIIKGDPRISS 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            ANVIWIS DSTSW+RNPSRWQDGELALD+CLEKSAVK++GDAWRNV+DCCLP+IHLIDTR
Sbjct: 1049 ANVIWISSDSTSWERNPSRWQDGELALDVCLEKSAVKEDGDAWRNVLDCCLPIIHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RS+PYAIKQVQ+LLGISCAFDQT+QRL+KSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSVPYAIKQVQKLLGISCAFDQTIQRLSKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPRRCFERAA KCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRRCFERAATKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            +FDILWDQKELG KQ DV+DVYNFLHMVRS KSEE TSACLG EIDDLMVEDEYGELTLS
Sbjct: 1229 KFDILWDQKELGSKQADVVDVYNFLHMVRSGKSEESTSACLGVEIDDLMVEDEYGELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            PEPFSTSEKPVFEDSAEFE+CLDN+            GA S GGGQWE+NEN+K   +S 
Sbjct: 1289 PEPFSTSEKPVFEDSAEFEHCLDNH----------SLGAASAGGGQWESNENSK---TSQ 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
            D+DWSGWG KV+PDV        TSKSGWD+TPSWGNKAT   +NDN WS   TKEVE D
Sbjct: 1349 DNDWSGWGTKVDPDV-------TTSKSGWDTTPSWGNKATK-ASNDNGWS---TKEVERD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFK-GETEPEKANVWSGWQNDKAETQDAFNKK 1440
            SF S +NTPK+GGWD+AATWG K KDVD+FK GET PEK+NVWSG Q++KAETQDAF+KK
Sbjct: 1409 SFTSTKNTPKTGGWDSAATWGMKTKDVDSFKEGETAPEKSNVWSGLQSNKAETQDAFHKK 1468

Query: 1441 IN--SRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKN 1500
            +   S+S G +DKAWS GTSKT DNWS++ KDKAE     VQEV   +NGW SAGGW KN
Sbjct: 1469 VEIASKSGGWDDKAWSRGTSKTEDNWSSRAKDKAEPWLAHVQEVSPNSNGWGSAGGWGKN 1528

Query: 1501 SGDADQSEACRNDGQASMDLETVADRWGSMATQRK-DSKDNFPSKAVEHGDSPLINHSWN 1560
            +GD D+SEA RNDGQASMDLE V+DRW     QR  DSKDNF SK VEHGDS  INHSW+
Sbjct: 1529 AGDGDESEAGRNDGQASMDLEKVSDRWDGRDVQRTGDSKDNFQSKVVEHGDSVAINHSWD 1588

Query: 1561 QHKSSEVFRGESGNDFWGQRKSQDVIKPS--------QGWGSQVKSNEGSSQNTQVERLW 1620
            Q K  EV +GE GND WGQ+KS +V KPS         GWGS+++ NEG +         
Sbjct: 1589 QQKPPEVSQGEYGNDAWGQQKSWEVKKPSHVNNESNRHGWGSRIELNEGPN--------- 1648

Query: 1621 SSQNESDQVASEHKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQND------------ 1680
               +E DQV     ++DS GWDSQ++++KPW+KQKS EASQSW SQ D            
Sbjct: 1649 ---HECDQV-----TNDSGGWDSQKQMDKPWEKQKSTEASQSWGSQKDSQSWGSQKDSQS 1708

Query: 1681 --------------------------------------------SMGSWGQLQRESEEFS 1740
                                                        S GSWGQLQR  +EFS
Sbjct: 1709 WGSQKDSQSWGSQKDSQSWGTQKDSQSWGSQKDSQSWGSLKDSQSQGSWGQLQRTPKEFS 1768

Query: 1741 QGSQDDSNKQFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSHSWGSHKES 1800
            Q SQDDSNK F   QK PE S GW   K       SHGWGSH +SS+  +SH W +    
Sbjct: 1769 QESQDDSNKHFDN-QKPPETSSGWEQQKSPE---VSHGWGSHIDSSDSTSSHGWDN---- 1828

Query: 1801 SELTTSHAWEKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMF 1860
                      KKNQGSK WG NVGEWKNRKNRPPKSPG+ +DDA LR +YTASGQRLDMF
Sbjct: 1829 ----------KKNQGSKSWGGNVGEWKNRKNRPPKSPGMTSDDANLRGLYTASGQRLDMF 1888

Query: 1861 TTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGI 1920
            TTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQ+VFNFHPDKAVKMGAGI
Sbjct: 1889 TTEEQDILADIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQSVFNFHPDKAVKMGAGI 1948

Query: 1921 DHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRS 1948
            DHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNF+KGKYPD+AE FVAKYFRKPRS
Sbjct: 1949 DHFMVSRHSSFQESRCFYVVSTDGHKEDFSYRKCLDNFIKGKYPDMAEMFVAKYFRKPRS 1982

BLAST of MS018728 vs. ExPASy TrEMBL
Match: A0A6J1JRG8 (DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111486902 PE=3 SV=1)

HSP 1 Score: 3212.2 bits (8327), Expect = 0.0e+00
Identity = 1641/2006 (81.80%), Postives = 1740/2006 (86.74%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CIAAISDCPITHASQLSNPFLGLPIE+GKCESCGTSEPGKCEGHFGYIELPIPI+HPNHI
Sbjct: 29   CIAAISDCPITHASQLSNPFLGLPIEYGKCESCGTSEPGKCEGHFGYIELPIPIYHPNHI 88

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
            TELKKMLSLLCLKCLKMKKNKFPSKN+GFAERLL SCCEDASQVSIRE KK+DGASYLQL
Sbjct: 89   TELKKMLSLLCLKCLKMKKNKFPSKNVGFAERLL-SCCEDASQVSIREAKKSDGASYLQL 148

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            KVPSRT LREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNE RKKLAGKGYYPQD
Sbjct: 149  KVPSRTSLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNETRKKLAGKGYYPQD 208

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GY+LQYLPVPPNCLSVPEISDGVTIMSSDPAV MLKK+LKQVEIIKGSRSGAPNFE+HEV
Sbjct: 209  GYVLQYLPVPPNCLSVPEISDGVTIMSSDPAVLMLKKVLKQVEIIKGSRSGAPNFEAHEV 268

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EANDLQ+AVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS
Sbjct: 269  EANDLQMAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 328

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNI YLQELVD KLCLTYRDGSSAYSL
Sbjct: 329  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNIKYLQELVDNKLCLTYRDGSSAYSL 388

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG
Sbjct: 389  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 448

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PL ADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQL  DSLLSLKMMFR
Sbjct: 449  PLGADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLANDSLLSLKMMFR 508

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
             YFLGKAAAQQLAMFV+SSLP PA+LG RS++ HWTALQILQTVLPACFDCHGDSYLIKN
Sbjct: 509  KYFLGKAAAQQLAMFVTSSLPPPALLGVRSNTLHWTALQILQTVLPACFDCHGDSYLIKN 568

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            SDFLKFDFDRDAMPSLINEIVTSIFFQ G EEV+RFFDSLQPLLMEH+FSEGFSV LDDY
Sbjct: 569  SDFLKFDFDRDAMPSLINEIVTSIFFQKGSEEVMRFFDSLQPLLMEHVFSEGFSVSLDDY 628

Query: 601  SMPMALLQALQKNIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 660
            SMPMA LQALQKNIQVISPLLYQLRS+FNELVELQLENHIRSVKVPFTNFILKLSSLGKL
Sbjct: 629  SMPMAFLQALQKNIQVISPLLYQLRSSFNELVELQLENHIRSVKVPFTNFILKLSSLGKL 688

Query: 661  FDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFGL 720
            FDSKSD+AINKVVQQIGFLGLQLSDKGKFYSKTLI+DVASLFHNRY SDK DYPSAEFGL
Sbjct: 689  FDSKSDAAINKVVQQIGFLGLQLSDKGKFYSKTLIDDVASLFHNRYSSDKNDYPSAEFGL 748

Query: 721  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 780
            VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV
Sbjct: 749  VKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNV 808

Query: 781  CSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMMK 840
            CSNSIIQLEYG+KAGMMKP+ LFPPGEPVGVLAATAMS PAYKAVLDSTPSS SSWDMMK
Sbjct: 809  CSNSIIQLEYGIKAGMMKPYGLFPPGEPVGVLAATAMSTPAYKAVLDSTPSSNSSWDMMK 868

Query: 841  EILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIEY 900
            EILLCKV FKNEP+DRRVILYLNNC CGRKHCNENAAY+VKSHLKKVTLKD  +DFMIEY
Sbjct: 869  EILLCKVGFKNEPVDRRVILYLNNCDCGRKHCNENAAYVVKSHLKKVTLKDVAMDFMIEY 928

Query: 901  NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHALRFS 960
            NRQ T S  GPGLVGHVHLN++LL+EL+INMADV RRC+ETISSF KKKKKK A  LRF 
Sbjct: 929  NRQPTPSALGPGLVGHVHLNQVLLEELRINMADVLRRCQETISSF-KKKKKKLAPTLRFF 988

Query: 961  FSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIKGDPRISA 1020
             SE+CSFHQ NGE+ TDMPCL FW ETRD HLERT+HI AD+VFPLLSETIIKGDPRIS+
Sbjct: 989  ISEHCSFHQRNGEERTDMPCLTFWLETRDVHLERTSHILADVVFPLLSETIIKGDPRISS 1048

Query: 1021 ANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPVIHLIDTR 1080
            ANVIWIS DSTSW+RNPSRWQDGELALD+CLEKSAVK++GDAWRNV+DCCLP+IHLIDTR
Sbjct: 1049 ANVIWISSDSTSWERNPSRWQDGELALDVCLEKSAVKEDGDAWRNVLDCCLPIIHLIDTR 1108

Query: 1081 RSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1140
            RS+PYAIKQVQ+LLGISCAFDQT+QRL+KSVSMVSKGVLGDHLILLANSMTCTGNMIGFN
Sbjct: 1109 RSVPYAIKQVQKLLGISCAFDQTIQRLSKSVSMVSKGVLGDHLILLANSMTCTGNMIGFN 1168

Query: 1141 SGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKHVAVGTGS 1200
            SGGYKALSRALNIQVPFTEATLFTPRRCFERAA KCHKDSLSSIVASCSWGKHVAVGTGS
Sbjct: 1169 SGGYKALSRALNIQVPFTEATLFTPRRCFERAATKCHKDSLSSIVASCSWGKHVAVGTGS 1228

Query: 1201 RFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDEYGELTLS 1260
            +FDILWDQKELG KQ DV+DVYNFLHMVRS KSEE TSACLG EIDDLMVEDEYGELTLS
Sbjct: 1229 KFDILWDQKELGSKQADVVDVYNFLHMVRSGKSEESTSACLGVEIDDLMVEDEYGELTLS 1288

Query: 1261 PEPFSTSEKPVFEDSAEFENCLDNYPGESKWEKAPPSGAGSTGGGQWENNENTKATNSSN 1320
            P+PFSTSEKPVFEDSAEFE+CLDN+            GA S GGGQWE+NEN K   +S 
Sbjct: 1289 PDPFSTSEKPVFEDSAEFEHCLDNH----------SLGAASAGGGQWESNENCK---TSQ 1348

Query: 1321 DHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATNTTTNDNDWSNSATKEVEPD 1380
            D+DWSGWG KV+PDV        TSKSGWD+TPSWGNKAT   +NDN WS   +KEVE D
Sbjct: 1349 DNDWSGWGTKVDPDV-------TTSKSGWDTTPSWGNKATK-ASNDNGWS---SKEVEQD 1408

Query: 1381 SFNSMENTPKSGGWDTAATWGTKAKDVDNFK-GETEPEKANVWSGWQNDKAETQDAFNKK 1440
            SF S +NTPK+GGWD+AATWGTK KDVD+FK GET PEK+NVWSG Q++KAETQDAF+KK
Sbjct: 1409 SFTSTKNTPKTGGWDSAATWGTKTKDVDSFKEGETAPEKSNVWSGLQSNKAETQDAFHKK 1468

Query: 1441 IN--SRSCGSEDKAWSTGTSKTYDNWSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKN 1500
            +   S+S G +DKAWS GTSKT DNWS++ KDKAE  Q  VQEV   +NGW SAGGW KN
Sbjct: 1469 VEIASKSGGWDDKAWSRGTSKTEDNWSSRAKDKAEPWQAHVQEVSPNSNGWGSAGGWGKN 1528

Query: 1501 SGDADQSEACRNDGQASMDLETVADRWGSMATQRK-DSKDNFPSKAVEHGDSPLINHSWN 1560
            +GD  +S A  NDGQ SMDLE V+DRW     QR  DS+DNF SK VE GDS  INHSW+
Sbjct: 1529 AGDG-ESGAGWNDGQTSMDLEKVSDRWDGRDVQRTGDSEDNFQSKVVELGDSVAINHSWD 1588

Query: 1561 QHKSSEVFRGESGNDFWGQRKSQDVIKPS--------QGWGSQVKSNEGSSQNTQVERLW 1620
            Q K  EV +GE GND WGQ+KS +V KPS         GWGS+++ NEG +         
Sbjct: 1589 QQKPPEVSQGEYGNDAWGQQKSWEVKKPSHVNNESNRHGWGSRIELNEGPN--------- 1648

Query: 1621 SSQNESDQVASEHKSSDSRGWDSQEKLNKPWDKQKSLEASQSWSSQND------------ 1680
               +E DQV     +SDS GWDSQ+K++KPW+KQKS EASQSW SQ D            
Sbjct: 1649 ---HECDQV-----TSDSGGWDSQKKMDKPWEKQKSTEASQSWGSQKDSQSWGSQKDSQS 1708

Query: 1681 -----------------------------------SMGSWGQLQRESEEFSQGSQDDSNK 1740
                                               S GSWGQLQR  +EFSQ SQDDSNK
Sbjct: 1709 WGSQKDSQSWGSQKDSQSWGSQKDSQSWGSQKDSHSQGSWGQLQRTPKEFSQESQDDSNK 1768

Query: 1741 QFSQVQKSPEVSHGWGSHKESSELTTSHGWGSHKESSELATSHSWGSHKESSELTTSHAW 1800
             F   QK PE S GW   K       SHGWGSH +SS+  +SH W +             
Sbjct: 1769 HFDN-QKPPETSSGWEQQKSPE---VSHGWGSHIDSSDSTSSHGWDN------------- 1828

Query: 1801 EKKNQGSKGWGANVGEWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDILA 1860
             KKNQGSK WG NVGEWKNRKNRPPKSPG+ +DDA LR +YTASGQRLDMFTTEEQDILA
Sbjct: 1829 -KKNQGSKSWGGNVGEWKNRKNRPPKSPGMTSDDANLRGLYTASGQRLDMFTTEEQDILA 1888

Query: 1861 DIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRHS 1920
            DIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQ+VFNFHPDKAVKMGAGIDHFMVSRHS
Sbjct: 1889 DIEPIMQSIRKIMHQSGYNDGDPLSAEDQSFILQSVFNFHPDKAVKMGAGIDHFMVSRHS 1948

Query: 1921 SFQESRCFYVVSTDGHKEDFSYRKCLDNFVKGKYPDIAEPFVAKYFRKPRSGKPRDRNSA 1948
            SFQESRCFYVVSTDGHKEDFSYRKCLDNF+KGKYPD+AE FVAKYFRKPRS KPRDRN+A
Sbjct: 1949 SFQESRCFYVVSTDGHKEDFSYRKCLDNFIKGKYPDMAEMFVAKYFRKPRSSKPRDRNTA 1972

BLAST of MS018728 vs. TAIR 10
Match: AT2G40030.1 (nuclear RNA polymerase D1B )

HSP 1 Score: 1798.1 bits (4656), Expect = 0.0e+00
Identity = 1005/1964 (51.17%), Postives = 1286/1964 (65.48%), Query Frame = 0

Query: 1    CIAAISDCPITHASQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHI 60
            CI +IS+  I H SQL+N FLGLP+EFGKCESCG +EP KCEGHFGYI+LP+PI+HP H+
Sbjct: 28   CIQSISESAINHPSQLTNAFLGLPLEFGKCESCGATEPDKCEGHFGYIQLPVPIYHPAHV 87

Query: 61   TELKKMLSLLCLKCLKMKKNKFPSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 120
             ELK+MLSLLCLKCLK+KK K  S   G A+RLL  CCE+ASQ+SI++ + +DGASYL+L
Sbjct: 88   NELKQMLSLLCLKCLKIKKAKGTSG--GLADRLLGVCCEEASQISIKD-RASDGASYLEL 147

Query: 121  KVPSRTPLREGFWDFLERYGFRYGDNLTRTLLPCEVKEMLKKIPNEARKKLAGKGYYPQD 180
            K+PSR+ L+ G W+FLERYG+RYG + TR LL  EVKE+L++IP E+RKKL  KG+ PQ+
Sbjct: 148  KLPSRSRLQPGCWNFLERYGYRYGSDYTRPLLAREVKEILRRIPEESRKKLTAKGHIPQE 207

Query: 181  GYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNFESHEV 240
            GYIL+YLPVPPNCLSVPE SDG + MS DP+   LK +LK+V  IK SRSG  NFESH+ 
Sbjct: 208  GYILEYLPVPPNCLSVPEASDGFSTMSVDPSRIELKDVLKKVIAIKSSRSGETNFESHKA 267

Query: 241  EANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRKGSGFSS 300
            EA+++   VD YLQVRGT KA+R ID RYGV+K  +  S+KAW EKMRTLFIRKGSGFSS
Sbjct: 268  EASEMFRVVDTYLQVRGTAKAARNIDMRYGVSKISDSSSSKAWTEKMRTLFIRKGSGFSS 327

Query: 301  RSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDKKLCLTYRDGSSAYSL 360
            RSVITGDAY+ VNE+G+P E+AQRITFEERVSVHN  YLQ+LVD KLCL+Y  GS+ YSL
Sbjct: 328  RSVITGDAYRHVNEVGIPIEIAQRITFEERVSVHNRGYLQKLVDDKLCLSYTQGSTTYSL 387

Query: 361  REGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICG 420
            R+GS GHT LKPGQ+VHRR+MDGD+VFINRPPTTHKHSLQALRVY+H+D+TVKINPL+C 
Sbjct: 388  RDGSKGHTELKPGQVVHRRVMDGDVVFINRPPTTHKHSLQALRVYVHEDNTVKINPLMCS 447

Query: 421  PLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLGTDSLLSLKMMFR 480
            PLSADFDGDC+HLFYPQS++AKAEV+ LFSVEKQLLSSH+G L LQ+G+DSLLSL++M  
Sbjct: 448  PLSADFDGDCVHLFYPQSLSAKAEVMELFSVEKQLLSSHTGQLILQMGSDSLLSLRVMLE 507

Query: 481  TYFLGKAAAQQLAMFVSSSLPSPAILGARSDSPHWTALQILQTVLPACFDCHGDSYLIKN 540
              FL KA AQQLAM+ S SLP PA+  +    P WT  QILQ   P    C GD +L+  
Sbjct: 508  RVFLDKATAQQLAMYGSLSLPPPALRKSSKSGPAWTVFQILQLAFPERLSCKGDRFLVDG 567

Query: 541  SDFLKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDY 600
            SD LKFDF  DAM S+INEIVTSIF + GP+E L FFDSLQPLLME +F+EGFS+ L+D 
Sbjct: 568  SDLLKFDFGVDAMGSIINEIVTSIFLEKGPKETLGFFDSLQPLLMESLFAEGFSLSLEDL 627

Query: 601  SMPMALLQALQK-NIQVISPLLYQLRSTFNELVELQLENHIRSVKVPFTNFILKLSSLGK 660
            SM  A +  +    I+ ISP++ +LR ++ +  ELQLEN I  VK    NF+LK  S+  
Sbjct: 628  SMSRADMDVIHNLIIREISPMVSRLRLSYRD--ELQLENSIHKVKEVAANFMLKSYSIRN 687

Query: 661  LFDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRYVSDKIDYPSAEFG 720
            L D KS+SAI K+VQQ GFLGLQLSDK KFY+KTL+ED+A     +Y   +I   S +FG
Sbjct: 688  LIDIKSNSAITKLVQQTGFLGLQLSDKKKFYTKTLVEDMAIFCKRKY--GRIS-SSGDFG 747

Query: 721  LVKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRN 780
            +VKGCFFHGLDPYEEM HSI+ REV+VRSSRGL EPGTLFKNLMA+LRD+VI  DGTVRN
Sbjct: 748  IVKGCFFHGLDPYEEMAHSIAAREVIVRSSRGLAEPGTLFKNLMAVLRDIVITNDGTVRN 807

Query: 781  VCSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVLDSTPSSTSSWDMM 840
             CSNS+IQ +YGV +       LF  GEPVGVLAATAMSNPAYKAVLDS+P+S SSW++M
Sbjct: 808  TCSNSVIQFKYGVDS-ERGHQGLFEAGEPVGVLAATAMSNPAYKAVLDSSPNSNSSWELM 867

Query: 841  KEILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHLKKVTLKDATVDFMIE 900
            KE+LLCKV+F+N   DRRVILYLN C CG++ C ENAA  V++ L KV+LKD  V+F++E
Sbjct: 868  KEVLLCKVNFQNTTNDRRVILYLNECHCGKRFCQENAACTVRNKLNKVSLKDTAVEFLVE 927

Query: 901  YNRQLTLS---GFGPGLVGHVHLNKMLLKELKINMADVSRRCEETISSFRKKKKKKFAHA 960
            Y +Q T+S   G    L GH+HLNK LL++  I+M D+ ++CE+ I+S  +KKKKK    
Sbjct: 928  YRKQPTISEIFGIDSCLHGHIHLNKTLLQDWNISMQDIHQKCEDVINSLGQKKKKKATDD 987

Query: 961  LR---FSFSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFADIVFPLLSETIIK 1020
             +    S SE CSF    G   +DMPCL F +   D  LERT  +  + V+P+L E +IK
Sbjct: 988  FKRTSLSVSECCSFRDPCGSKGSDMPCLTFSYNATDPDLERTLDVLCNTVYPVLLEIVIK 1047

Query: 1021 GDPRISAANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNGDAWRNVMDCCLPV 1080
            GD RI +AN+IW S D T+W RN    + GE  LD+ +EKSAVKQ+GDAWR V+D CL V
Sbjct: 1048 GDSRICSANIIWNSSDMTTWIRNRHASRRGEWVLDVTVEKSAVKQSGDAWRVVIDSCLSV 1107

Query: 1081 IHLIDTRRSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLGDHLILLANSMTCT 1140
            +HLIDT+RSIPY++KQVQELLG+SCAF+Q VQRL+ SV MVSKGVL +H+ILLAN+MTC+
Sbjct: 1108 LHLIDTKRSIPYSVKQVQELLGLSCAFEQAVQRLSASVRMVSKGVLKEHIILLANNMTCS 1167

Query: 1141 GNMIGFNSGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDSLSSIVASCSWGKH 1200
            G M+GFNSGGYKAL+R+LNI+ PFTEATL  PR+CFE+AAEKCH DSLS++V SCSWGK 
Sbjct: 1168 GTMLGFNSGGYKALTRSLNIKAPFTEATLIAPRKCFEKAAEKCHTDSLSTVVGSCSWGKR 1227

Query: 1201 VAVGTGSRFDILWDQKELGCKQDDVLDVYNFLHMVRSAKSEELTSACLGEEIDDLMVEDE 1260
            V VGTGS+F++LW+QKE G    +  DVY+FL MV S  + +   +  G ++     E+E
Sbjct: 1228 VDVGTGSQFELLWNQKETGLDDKEETDVYSFLQMVISTTNADAFVSSPGFDV----TEEE 1287

Query: 1261 YGELTLSPEPFSTSEKPVFEDSAEFENCLD-NYPGESKWEKAPPSGAGSTGGGQWENNEN 1320
              E   SPE  S   +P FEDSA+F+N  D   P  + WEK+     G +GG +W  +++
Sbjct: 1288 MAEWAESPERDSALGEPKFEDSADFQNLHDEGKPSGANWEKSSSWDNGCSGGSEWGVSKS 1347

Query: 1321 T-----------KATNSSNDHDWSGWGRKVEPDVVTTKAQENTSKSGWDSTPSWGNKATN 1380
            T           K TN   +  WS W         T K  + +SKS  DS  +WG K   
Sbjct: 1348 TGGEANPESNWEKTTNVEKEDAWSSWN--------TRKDAQESSKS--DSGGAWGIK--- 1407

Query: 1381 TTTNDNDWSNSATKEVEP---DSFNSMENTPKSGGWDTAATWGTKAKDVDNFKGETEP-- 1440
              T D D   +   E  P   DS     N P S  W   +    K+ D  N+  E+ P  
Sbjct: 1408 --TKDADADTTPNWETSPAPKDSIVPENNEPTSDVWGHKSV-SDKSWDKKNWGTESAPAA 1467

Query: 1441 ---EKANVWSGWQNDKAETQDAFNKKINSRSCGSEDK-------------AWSTGTSKTY 1500
                 A VW       +ET+       ++ + GS DK              W+  +S+T 
Sbjct: 1468 WGSTDAAVWGSSDKKNSETES------DAAAWGSRDKNNSDVGSGAGVLGPWNKKSSETE 1527

Query: 1501 DN---WSNQVKDKAESCQVQVQEVPSKTNGWDSAGGWQKNSGDADQSEACRNDGQASMDL 1560
             N   W +  K K+ +         +  N WD     +KN     +  A  + G+ + + 
Sbjct: 1528 SNGATWGSSDKTKSGA---------AAWNSWD-----KKNIETDSEPAAWGSQGKKNSET 1587

Query: 1561 ETVADRWGSMATQRKDSKDNFPSKAVEHGDSPLINHSWNQHKSSEVFRGESGNDFWGQRK 1620
            E+    WG+   ++ +++   P  A                K+SE   G +    W ++K
Sbjct: 1588 ESGPAAWGAWDKKKSETE---PGPA---------GWGMGDKKNSETELGPAAMGNWDKKK 1647

Query: 1621 SQDVIKPSQGWGSQVKSNEGSSQNTQVERLWSSQNESDQVASEHKSSDSRGWDSQEKLNK 1680
            S     P+  WGS   +  GSS         +S+ ESD  A          W S+ K   
Sbjct: 1648 SDTKSGPA-AWGSTDAAAWGSSDKN------NSETESDAAA----------WGSRNK--- 1707

Query: 1681 PWDKQKSLEASQSWSSQNDSMGSWGQLQRESEEFSQGSQDDSNKQFSQVQKSPEVSHGWG 1740
               K   +E+         + GSWGQ    +E+    ++DD N               W 
Sbjct: 1708 ---KTSEIESGAG------AWGSWGQPSPTAED-KDTNEDDRNP--------------WV 1767

Query: 1741 SHKESSELTTSHGWGSHKESSELATSHSWGSHKESSELTTSHAWEKKNQGSKGWGANVG- 1800
            S KE+            K+  E +    WG+              KK   S GW    G 
Sbjct: 1768 SLKETK--------SREKDDKERS---QWGNP------------AKKFPSSGGWSNGGGA 1827

Query: 1801 EWKNRKNRPPKSPGILNDDAGLRAIYTASGQRLDMFTTEEQDILADIEPIMQSIRKIMHQ 1860
            +WK  +N  P+ P     +  L  ++TA+ QRLD FT+EEQ++L+D+EP+M+++RKIMH 
Sbjct: 1828 DWKGNRNHTPRPP---RSEDNLAPMFTATRQRLDSFTSEEQELLSDVEPVMRTLRKIMHP 1860

Query: 1861 SGYNDGDPLSAEDQSFILQNVFNFHPDKAVKMGAGIDHFMVSRHSSFQESRCFYVVSTDG 1920
            S Y DGDP+S +D++F+L+ + NFHP K  K+G+G+D   V +H+ F +SRCF+VVSTDG
Sbjct: 1888 SAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFITVDKHTIFSDSRCFFVVSTDG 1860

BLAST of MS018728 vs. TAIR 10
Match: AT1G63020.1 (nuclear RNA polymerase D1A )

HSP 1 Score: 377.1 bits (967), Expect = 8.6e-104
Identity = 343/1312 (26.14%), Postives = 575/1312 (43.83%), Query Frame = 0

Query: 14   SQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHITELKKMLSLLCLK 73
            +Q+++  LGLP     C +CG+ +   CEGHFG I     I +P  + E+  +L+ +C  
Sbjct: 40   NQVTDSRLGLPNPDSVCRTCGSKDRKVCEGHFGVINFAYSIINPYFLKEVAALLNKICPG 99

Query: 74   CLKMKKNKFP------------SKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQLK 133
            C  ++K +F             + N G+         ++  + S   + + +  S ++LK
Sbjct: 100  CKYIRKKQFQITEDQPERCRYCTLNTGYPLMKFRVTTKEVFRRS-GIVVEVNEESLMKLK 159

Query: 134  VPSRTPLREGFWDFLERYGFRYGDNL---TRTLLPCEVKEMLKKIPNEARKKLAGKGYYP 193
                  L   +W FL +        L    R +   +V  +L  I     ++L  K    
Sbjct: 160  KRGVLTLPPDYWSFLPQDSNIDESCLKPTRRIITHAQVYALLLGID----QRLIKKDIPM 219

Query: 194  QDGYILQYLPVPPNCLSVPEI---SDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNF 253
             +   L   PV PN   V EI    +G  ++  D    + KK++               F
Sbjct: 220  FNSLGLTSFPVTPNGYRVTEIVHQFNGARLI-FDERTRIYKKLV--------------GF 279

Query: 254  ESHEVEANDLQLAVDQYLQV-RGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRK 313
            E + +E +   +   QY ++   TV +S+     Y   ++ +D      L  M+ + + K
Sbjct: 280  EGNTLELSSRVMECMQYSRLFSETVSSSKDSANPY---QKKSDTPKLCGLRFMKDVLLGK 339

Query: 314  GSGFSSRSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHN-----INYLQELVDKKLCL 373
             S  + R+V+ GD    +NEIG+P  +A+R+   E ++  N      +++  L+D K  +
Sbjct: 340  RSDHTFRTVVVGDPSLKLNEIGIPESIAKRLQVSEHLNQCNKERLVTSFVPTLLDNKE-M 399

Query: 374  TYRDGSSAYSLREGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRV-YLHD 433
              R G    +++        L+ G  + R +MDGD V +NRPP+ H+HSL A+ V  L  
Sbjct: 400  HVRRGDRLVAIQVND-----LQTGDKIFRSLMDGDTVLMNRPPSIHQHSLIAMTVRILPT 459

Query: 434  DHTVKINPLICGPLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLG 493
               V +NP+ C P   DFDGDC+H + PQSI AK E+  L +++KQL++  +G   L LG
Sbjct: 460  TSVVSLNPICCLPFRGDFDGDCLHGYVPQSIQAKVELDELVALDKQLINRQNGRNLLSLG 519

Query: 494  TDSLLS--LKMMFRTYFLGKAAAQQLAMFVSSSLPSPAILGA--RSDSPHWTALQILQTV 553
             DSL +  L  + +  +L +A  QQL M+    LP PAI+ A   S  P WT +Q+   +
Sbjct: 520  QDSLTAAYLVNVEKNCYLNRAQMQQLQMYCPFQLPPPAIIKASPSSTEPQWTGMQLFGML 579

Query: 554  LPACFDCHG--DSYLIKNSDFLKFD----FDRDAMPSLINEIVTSIFFQNGPEEVLRFFD 613
             P  FD     ++ ++ N + L F     + RD   + I  ++     ++   +VL    
Sbjct: 580  FPPGFDYTYPLNNVVVSNGELLSFSEGSAWLRDGEGNFIERLL-----KHDKGKVLDIIY 639

Query: 614  SLQPLLMEHIFSEGFSVGLDDYSMPMALLQALQKNIQVISPLLYQLR------------- 673
            S Q +L + +   G SV L D    + L   LQ    +   + Y LR             
Sbjct: 640  SAQEMLSQWLLMRGLSVSLAD----LYLSSDLQSRKNLTEEISYGLREAEQVCNKQQLMV 699

Query: 674  --------------------------------STFNELVELQLENHIRSVKVPFTNFILK 733
                                            +T +EL     ++  R V+     +  +
Sbjct: 700  ESWRDFLAVNGEDKEEDSVSDLARFCYERQKSATLSELAVSAFKDAYRDVQALAYRYGDQ 759

Query: 734  LSSLGKLFDSKSDSAINKVVQQIGFLGLQLSDKGKFY------SKTLIEDVASLFHNRYV 793
             +S   +  + S   I K+VQ    +GLQ S     +      +     D  S       
Sbjct: 760  SNSFLIMSKAGSKGNIGKLVQHSMCIGLQNSAVSLSFGFPRELTCAAWNDPNSPLRGAKG 819

Query: 794  SDKIDYPS-AEFGLVKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAIL 853
             D     S   +G+++  F  GL+P E  VHS+++R+     +  L  PGTL + LM  +
Sbjct: 820  KDSTTTESYVPYGVIENSFLTGLNPLESFVHSVTSRDSSFSGNADL--PGTLSRRLMFFM 879

Query: 854  RDVVICYDGTVRNVCSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVL 913
            RD+   YDGTVRN   N ++Q  Y     +         GE +G L+A A+S  AY A L
Sbjct: 880  RDIYAAYDGTVRNSFGNQLVQFTYETDGPVED-----ITGEALGSLSACALSEAAYSA-L 939

Query: 914  DSTPS--STSSWDMMKEILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHL 973
            D   S   TS    +K +L C    K    ++ + LYL+     +KH  E  +  +K+HL
Sbjct: 940  DQPISLLETSPLLNLKNVLEC--GSKKGQREQTMSLYLSEYLSKKKHGFEYGSLEIKNHL 999

Query: 974  KKVTLKDATVDFMIEY----NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEE 1033
            +K++  +     MI +    N ++ LS +    V H H+++ +LK  +++   V     E
Sbjct: 1000 EKLSFSEIVSTSMIIFSPSSNTKVPLSPW----VCHFHISEKVLKRKQLSAESVVSSLNE 1059

Query: 1034 TISSFRKKKKKKFAHALRFSFSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFA 1093
               S R ++ K     L    + +CS      +D  D  C+         H         
Sbjct: 1060 QYKS-RNRELKLDIVDLDIQNTNHCSSDDQAMKD--DNVCITVTVVEASKHSVLELDAIR 1119

Query: 1094 DIVFPLLSETIIKGDPRISAANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNG 1153
             ++ P L ++ +KGD  I   N++W   D     +       GEL L + +     K+N 
Sbjct: 1120 LVLIPFLLDSPVKGDQGIKKVNILW--TDRPKAPKRNGNHLAGELYLKVTMYGDRGKRN- 1179

Query: 1154 DAWRNVMDCCLPVIHLIDTRRSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLG 1213
              W  +++ CLP++ +ID  RS P  I+Q   + GI       V  L  +VS   K +L 
Sbjct: 1180 -CWTALLETCLPIMDMIDWGRSHPDNIRQCCSVYGIDAGRSIFVANLESAVSDTGKEILR 1239

Query: 1214 DHLILLANSMTCTGNMIGFNSGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDS 1233
            +HL+L+A+S++ TG  +  N+ G+    +  +   PFT+A   +P +CF +AA++  +D 
Sbjct: 1240 EHLLLVADSLSVTGEFVALNAKGWSKQRQVESTPAPFTQACFSSPSQCFLKAAKEGVRDD 1290

BLAST of MS018728 vs. TAIR 10
Match: AT1G63020.2 (nuclear RNA polymerase D1A )

HSP 1 Score: 377.1 bits (967), Expect = 8.6e-104
Identity = 343/1312 (26.14%), Postives = 575/1312 (43.83%), Query Frame = 0

Query: 14   SQLSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHITELKKMLSLLCLK 73
            +Q+++  LGLP     C +CG+ +   CEGHFG I     I +P  + E+  +L+ +C  
Sbjct: 40   NQVTDSRLGLPNPDSVCRTCGSKDRKVCEGHFGVINFAYSIINPYFLKEVAALLNKICPG 99

Query: 74   CLKMKKNKFP------------SKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQLK 133
            C  ++K +F             + N G+         ++  + S   + + +  S ++LK
Sbjct: 100  CKYIRKKQFQITEDQPERCRYCTLNTGYPLMKFRVTTKEVFRRS-GIVVEVNEESLMKLK 159

Query: 134  VPSRTPLREGFWDFLERYGFRYGDNL---TRTLLPCEVKEMLKKIPNEARKKLAGKGYYP 193
                  L   +W FL +        L    R +   +V  +L  I     ++L  K    
Sbjct: 160  KRGVLTLPPDYWSFLPQDSNIDESCLKPTRRIITHAQVYALLLGID----QRLIKKDIPM 219

Query: 194  QDGYILQYLPVPPNCLSVPEI---SDGVTIMSSDPAVSMLKKILKQVEIIKGSRSGAPNF 253
             +   L   PV PN   V EI    +G  ++  D    + KK++               F
Sbjct: 220  FNSLGLTSFPVTPNGYRVTEIVHQFNGARLI-FDERTRIYKKLV--------------GF 279

Query: 254  ESHEVEANDLQLAVDQYLQV-RGTVKASRGIDARYGVNKELNDPSTKAWLEKMRTLFIRK 313
            E + +E +   +   QY ++   TV +S+     Y   ++ +D      L  M+ + + K
Sbjct: 280  EGNTLELSSRVMECMQYSRLFSETVSSSKDSANPY---QKKSDTPKLCGLRFMKDVLLGK 339

Query: 314  GSGFSSRSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHN-----INYLQELVDKKLCL 373
             S  + R+V+ GD    +NEIG+P  +A+R+   E ++  N      +++  L+D K  +
Sbjct: 340  RSDHTFRTVVVGDPSLKLNEIGIPESIAKRLQVSEHLNQCNKERLVTSFVPTLLDNKE-M 399

Query: 374  TYRDGSSAYSLREGSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKHSLQALRV-YLHD 433
              R G    +++        L+ G  + R +MDGD V +NRPP+ H+HSL A+ V  L  
Sbjct: 400  HVRRGDRLVAIQVND-----LQTGDKIFRSLMDGDTVLMNRPPSIHQHSLIAMTVRILPT 459

Query: 434  DHTVKINPLICGPLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLSSHSGNLNLQLG 493
               V +NP+ C P   DFDGDC+H + PQSI AK E+  L +++KQL++  +G   L LG
Sbjct: 460  TSVVSLNPICCLPFRGDFDGDCLHGYVPQSIQAKVELDELVALDKQLINRQNGRNLLSLG 519

Query: 494  TDSLLS--LKMMFRTYFLGKAAAQQLAMFVSSSLPSPAILGA--RSDSPHWTALQILQTV 553
             DSL +  L  + +  +L +A  QQL M+    LP PAI+ A   S  P WT +Q+   +
Sbjct: 520  QDSLTAAYLVNVEKNCYLNRAQMQQLQMYCPFQLPPPAIIKASPSSTEPQWTGMQLFGML 579

Query: 554  LPACFDCHG--DSYLIKNSDFLKFD----FDRDAMPSLINEIVTSIFFQNGPEEVLRFFD 613
             P  FD     ++ ++ N + L F     + RD   + I  ++     ++   +VL    
Sbjct: 580  FPPGFDYTYPLNNVVVSNGELLSFSEGSAWLRDGEGNFIERLL-----KHDKGKVLDIIY 639

Query: 614  SLQPLLMEHIFSEGFSVGLDDYSMPMALLQALQKNIQVISPLLYQLR------------- 673
            S Q +L + +   G SV L D    + L   LQ    +   + Y LR             
Sbjct: 640  SAQEMLSQWLLMRGLSVSLAD----LYLSSDLQSRKNLTEEISYGLREAEQVCNKQQLMV 699

Query: 674  --------------------------------STFNELVELQLENHIRSVKVPFTNFILK 733
                                            +T +EL     ++  R V+     +  +
Sbjct: 700  ESWRDFLAVNGEDKEEDSVSDLARFCYERQKSATLSELAVSAFKDAYRDVQALAYRYGDQ 759

Query: 734  LSSLGKLFDSKSDSAINKVVQQIGFLGLQLSDKGKFY------SKTLIEDVASLFHNRYV 793
             +S   +  + S   I K+VQ    +GLQ S     +      +     D  S       
Sbjct: 760  SNSFLIMSKAGSKGNIGKLVQHSMCIGLQNSAVSLSFGFPRELTCAAWNDPNSPLRGAKG 819

Query: 794  SDKIDYPS-AEFGLVKGCFFHGLDPYEEMVHSISTREVMVRSSRGLTEPGTLFKNLMAIL 853
             D     S   +G+++  F  GL+P E  VHS+++R+     +  L  PGTL + LM  +
Sbjct: 820  KDSTTTESYVPYGVIENSFLTGLNPLESFVHSVTSRDSSFSGNADL--PGTLSRRLMFFM 879

Query: 854  RDVVICYDGTVRNVCSNSIIQLEYGVKAGMMKPHNLFPPGEPVGVLAATAMSNPAYKAVL 913
            RD+   YDGTVRN   N ++Q  Y     +         GE +G L+A A+S  AY A L
Sbjct: 880  RDIYAAYDGTVRNSFGNQLVQFTYETDGPVED-----ITGEALGSLSACALSEAAYSA-L 939

Query: 914  DSTPS--STSSWDMMKEILLCKVSFKNEPIDRRVILYLNNCACGRKHCNENAAYLVKSHL 973
            D   S   TS    +K +L C    K    ++ + LYL+     +KH  E  +  +K+HL
Sbjct: 940  DQPISLLETSPLLNLKNVLEC--GSKKGQREQTMSLYLSEYLSKKKHGFEYGSLEIKNHL 999

Query: 974  KKVTLKDATVDFMIEY----NRQLTLSGFGPGLVGHVHLNKMLLKELKINMADVSRRCEE 1033
            +K++  +     MI +    N ++ LS +    V H H+++ +LK  +++   V     E
Sbjct: 1000 EKLSFSEIVSTSMIIFSPSSNTKVPLSPW----VCHFHISEKVLKRKQLSAESVVSSLNE 1059

Query: 1034 TISSFRKKKKKKFAHALRFSFSENCSFHQSNGEDSTDMPCLIFWHETRDSHLERTAHIFA 1093
               S R ++ K     L    + +CS      +D  D  C+         H         
Sbjct: 1060 QYKS-RNRELKLDIVDLDIQNTNHCSSDDQAMKD--DNVCITVTVVEASKHSVLELDAIR 1119

Query: 1094 DIVFPLLSETIIKGDPRISAANVIWISPDSTSWQRNPSRWQDGELALDICLEKSAVKQNG 1153
             ++ P L ++ +KGD  I   N++W   D     +       GEL L + +     K+N 
Sbjct: 1120 LVLIPFLLDSPVKGDQGIKKVNILW--TDRPKAPKRNGNHLAGELYLKVTMYGDRGKRN- 1179

Query: 1154 DAWRNVMDCCLPVIHLIDTRRSIPYAIKQVQELLGISCAFDQTVQRLAKSVSMVSKGVLG 1213
              W  +++ CLP++ +ID  RS P  I+Q   + GI       V  L  +VS   K +L 
Sbjct: 1180 -CWTALLETCLPIMDMIDWGRSHPDNIRQCCSVYGIDAGRSIFVANLESAVSDTGKEILR 1239

Query: 1214 DHLILLANSMTCTGNMIGFNSGGYKALSRALNIQVPFTEATLFTPRRCFERAAEKCHKDS 1233
            +HL+L+A+S++ TG  +  N+ G+    +  +   PFT+A   +P +CF +AA++  +D 
Sbjct: 1240 EHLLLVADSLSVTGEFVALNAKGWSKQRQVESTPAPFTQACFSSPSQCFLKAAKEGVRDD 1290

BLAST of MS018728 vs. TAIR 10
Match: AT4G35800.1 (RNA polymerase II large subunit )

HSP 1 Score: 206.5 bits (524), Expect = 2.0e-52
Identity = 219/865 (25.32%), Postives = 368/865 (42.54%), Query Frame = 0

Query: 16  LSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHITELKKMLSLLCLKCL 75
           LS+  LG      KCE+C  +   +C GHFGY+EL  P++H   +  +  ++  +C  C 
Sbjct: 52  LSDTRLGTIDRKVKCETC-MANMAECPGHFGYLELAKPMYHVGFMKTVLSIMRCVCFNCS 111

Query: 76  KM----KKNKFPS----KN-IGFAERLLSSC-----CEDASQVS----------IREMKK 135
           K+    +++KF      KN     +++L +C     C+    +           +++ + 
Sbjct: 112 KILADEEEHKFKQAMKIKNPKNRLKKILDACKNKTKCDGGDDIDDVQSHSTDEPVKKSRG 171

Query: 136 ADGASYLQLKVPSRTPLREGFWDFLERYGFRYGDNL------TRTLLPCEVKEMLKKIPN 195
             GA   +L +     + E     ++R      D L       +TL    V  +LK+I +
Sbjct: 172 GCGAQQPKLTIEGMKMIAE---YKIQRKKNDEPDQLPEPAERKQTLGADRVLSVLKRISD 231

Query: 196 EARKKLAGKGYYPQ----DGYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQ 255
              + L   G+ P+    D  IL+ LP+PP  +  P +    T  S D     L  I++ 
Sbjct: 232 ADCQLL---GFNPKFARPDWMILEVLPIPPPPVR-PSVMMDATSRSEDDLTHQLAMIIRH 291

Query: 256 VEIIK-GSRSGAPNFESHEVEANDLQLAVDQYL------QVRGTVKASRGIDARYGVNKE 315
            E +K   ++GAP     E     LQ  +  Y       Q R T K+ R I +       
Sbjct: 292 NENLKRQEKNGAPAHIISEF-TQLLQFHIATYFDNELPGQPRATQKSGRPIKSICS---- 351

Query: 316 LNDPSTKAWLEKMRTLFIRKGSGFSSRSVITGDAYKLVNEIGVPFEVAQRITFEERVSVH 375
                 KA   ++R   + K   FS+R+VIT D    ++E+GVP+ +A  +T+ E V+ +
Sbjct: 352 ----RLKAKEGRIRGNLMGKRVDFSARTVITPDPTINIDELGVPWSIALNLTYPETVTPY 411

Query: 376 NINYLQELVD-------KKLCLTY--RDGSSAYSLRE-GSMGHTYLKPGQIVHRRIMDGD 435
           NI  L+ELVD        K    Y  RD      LR        +L+ G  V R + DGD
Sbjct: 412 NIERLKELVDYGPHPPPGKTGAKYIIRDDGQRLDLRYLKKSSDQHLELGYKVERHLQDGD 471

Query: 436 IVFINRPPTTHKHSLQALRVYLHDDHTVKINPLICGPLSADFDGDCIHLFYPQSIAAKAE 495
            V  NR P+ HK S+   R+ +    T ++N  +  P +ADFDGD +++  PQS   +AE
Sbjct: 472 FVLFNRQPSLHKMSIMGHRIRIMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSFETRAE 531

Query: 496 VLGLFSVEKQLLSSHSGNLNLQLGTDSLLSL-KMMFRTYFLGKAAAQQLAMF---VSSSL 555
           VL L  V K ++S  +    + +  D+LL   K+  R  F+ K       M+       +
Sbjct: 532 VLELMMVPKCIVSPQANRPVMGIVQDTLLGCRKITKRDTFIEKDVFMNTLMWWEDFDGKV 591

Query: 556 PSPAILGARSDSPHWTALQILQTVLP----------------ACFDCHGDSYL-IKNSDF 615
           P+PAIL  R   P WT  Q+   ++P                  F   GD+ + I+  + 
Sbjct: 592 PAPAILKPR---PLWTGKQVFNLIIPKQINLLRYSAWHADTETGFITPGDTQVRIERGEL 651

Query: 616 LKFDFDRDAMPSLINEIVTSIFFQNGPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDYSMP 675
           L     +  + +    +V  I+ + GP+   +F    Q L+   +   GF++G+ D    
Sbjct: 652 LAGTLCKKTLGTSNGSLVHVIWEEVGPDAARKFLGHTQWLVNYWLLQNGFTIGIGDTIAD 711

Query: 676 MALLQALQKNIQ----VISPLLYQ-------------LRSTFNELVELQLENHIRSVKVP 735
            + ++ + + I      +  L+ Q             +R TF   V   L          
Sbjct: 712 SSTMEKINETISNAKTAVKDLIRQFQGKELDPEPGRTMRDTFENRVNQVLNKARDDAGSS 771

Query: 736 FTNFILKLSSLGKLFDSKSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNRY 792
               + + ++L  +  + S  +   + Q    +G Q + +GK        D  +L H   
Sbjct: 772 AQKSLAETNNLKAMVTAGSKGSFINISQMTACVG-QQNVEGKRIPFGF--DGRTLPH--- 831

BLAST of MS018728 vs. TAIR 10
Match: AT5G60040.1 (nuclear RNA polymerase C1 )

HSP 1 Score: 153.3 bits (386), Expect = 2.0e-36
Identity = 204/866 (23.56%), Postives = 357/866 (41.22%), Query Frame = 0

Query: 16  LSNPFLGLPIEFGKCESCGTSEPGKCEGHFGYIELPIPIFHPNHITELKKMLSLLCLKC- 75
           L +P +G P +   C +C       C GH+GY++L +P+++  +   +  +L  +C +C 
Sbjct: 61  LLDPRMGPPNKKSICTTC-EGNFQNCPGHYGYLKLDLPVYNVGYFNFILDILKCICKRCS 120

Query: 76  -------------LKMKKNKF-PSKNIGFAERLLSSCCEDASQVSIREMKKADGASYLQL 135
                         KM+  +  P K    A+ ++  C   ASQ  I   KK    + +  
Sbjct: 121 NMLLDEKLYEDHLRKMRNPRMEPLKKTELAKAVVKKCSTMASQ-RIITCKKCGYLNGMVK 180

Query: 136 KVPS---------RTPLREGFWDFLE------RYGFRYGDNLTRTLLPCEVKEMLKKIPN 195
           K+ +         R+ +  G  D  +      +      + LT  L P  V  + K++ +
Sbjct: 181 KIAAQFGIGISHDRSKIHGGEIDECKSAISHTKQSTAAINPLTYVLDPNLVLGLFKRM-S 240

Query: 196 EARKKLAGKGYYPQDGYILQYLPVPPNCLSVPEISDGVTIMSSDPAVSMLKKILKQVEII 255
           +   +L    Y P++  I+  + VPP  +    +  G+    +D    + + IL    + 
Sbjct: 241 DKDCELLYIAYRPEN-LIITCMLVPPLSIRPSVMIGGIQSNENDLTARLKQIILGNASLH 300

Query: 256 KGSRSGAPNFESHEVEANDLQLAVDQYLQVRGTVKASRGIDARYGVNKELNDPSTKAWLE 315
           K       + ++ +V  + +Q+ V +Y  +   V+  +     + ++  L     K    
Sbjct: 301 KILSQPTSSPKNMQV-WDTVQIEVARY--INSEVRGCQNQPEEHPLSGILQRLKGKG--G 360

Query: 316 KMRTLFIRKGSGFSSRSVITGDAYKLVNEIGVPFEVAQRITFEERVSVHNINYLQELVDK 375
           + R     K   F+ R+VI+ D    + E+G+P  +AQ +TF E VS HNI  L++ V  
Sbjct: 361 RFRANLSGKRVEFTGRTVISPDPNLKITEVGIPILMAQILTFPECVSRHNIEKLRQCVRN 420

Query: 376 -------KLCLTYRDGSSAYSLRE-GSMGHTYLKPGQIVHRRIMDGDIVFINRPPTTHKH 435
                     + Y DGSS   + +        L  G IV R + +GD+V  NR P+ H+ 
Sbjct: 421 GPNKYPGARNVRYPDGSSRTLVGDYRKRIADELAIGCIVDRHLQEGDVVLFNRQPSLHRM 480

Query: 436 SLQALRVYLHDDHTVKINPLICGPLSADFDGDCIHLFYPQSIAAKAEVLGLFSVEKQLLS 495
           S+   R  +    T++ N  +C P +ADFDGD +++  PQ+  A+ E + L  V+  L +
Sbjct: 481 SIMCHRARIMPWRTLRFNESVCNPYNADFDGDEMNMHVPQTEEARTEAITLMGVQNNLCT 540

Query: 496 SHSGNLNLQLGTDSLLSLKMMFR-TYFLGKAAAQQLAMFV-----SSSLPSPAILGARSD 555
             +G + +    D L S  ++ R   F  +AA   +  ++     S  LP+P IL     
Sbjct: 541 PKNGEILVASTQDFLTSSFLITRKDTFYDRAAFSLICSYMGDGMDSIDLPTPTIL---KP 600

Query: 556 SPHWTALQILQTVL----------------------PACFD---CHGDSYL-IKNSDFLK 615
              WT  QI   +L                         FD   C  D ++  +NS+ + 
Sbjct: 601 IELWTGKQIFSVLLRPNASIRVYVTLNVKEKNFKKGEHGFDETMCINDGWVYFRNSELIS 660

Query: 616 FDFDRDAMPSLINEIVTSIFFQN-GPEEVLRFFDSLQPLLMEHIFSEGFSVGLDDYSMPM 675
               +  + +   + + SI  ++          + L  L    I   GFS+G+DD     
Sbjct: 661 GQLGKATLGNGNKDGLYSILLRDYNSHAAAVCMNRLAKLSARWIGIHGFSIGIDDVQPGE 720

Query: 676 ALLQALQKNIQVISPLLYQLRSTFNELVELQLE---NHIRSVKVPFTNFILKL-SSLGKL 735
            L +  + +IQ      ++    FN    LQL+   +  +S++   T  +  +  + GK 
Sbjct: 721 ELSKERKDSIQFGYDQCHRKIEEFNR-GNLQLKAGLDGAKSLEAEITGILNTIREATGKA 780

Query: 736 FDS---------------KSDSAINKVVQQIGFLGLQLSDKGKFYSKTLIEDVASLFHNR 792
             S                  S IN + Q +  +G Q +  G       I+   SL H  
Sbjct: 781 CMSGLHWRNSPLIMSQCGSKGSPIN-ISQMVACVG-QQTVNGHRAPDGFID--RSLPHFP 840