MS010365 (gene) Bitter gourd (TR) v1

Overview


Name	MS010365
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Protein kinase domain-containing protein
Location	scaffold878: 458132 .. 459715 (+)
RNA-Seq Expression	MS010365
Synteny	MS010365

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCCTTGTCGCCAAAATTCAAATTCAGTCCGGTGAGGTCATCATGAATGATCAAGAAGAACCCAAAAGGCCCCAATTCCCTCTCGATTCAAGCTCTTACAGCATCATCGACGAAATTGGGGTCGGCGTGAGCGCCGTCGTGTACAAAGCCATCTGCATTCCCATGAACTCCGCGGTGGTGGCTATCAAATCCATCGACCTCGACCGATCGCGGGCCGATTTTGACGATATCCGAAGGGAAGCCCAAATTCTGTCGCTTCTCTCTCACCCCAATATTCTCAACGCCCACTGTTCTTTCACCGTCGATCGTCGGCTCTGGGTCGTCATGCCGTTTATGTCCGCCGGGTCTCTGCAGTCCATCATTACGTCGTCGTTTCCCGACGGTTTGCCGGAGCCCTGCATCGCCGCCGTACTCCGGGAAATTCTGAGCGCCCTGGCTTATCTCCACGACCAAGGCCACCTCCACCGCGACATTAAAGCCGGCAACATTCTGATGGACTCAAACGGGTCCGTTAAGCTCGCGGATTTCGGAGTTTCCACTTCGATTTTCGAGGCCAATGCGAAACAGGGGTTGTCGACTTCTTCGTTGTCGGCGTCGATGATGAAGCTAACGGACGTCGCCGGAACGCCGTACTGGATGGCGCCGGAGGTGATCCACTCGCATAATGGGTATAGTTTGAAGGCGGATATATGGTCATTTGGGATCACGGCGTTGGAATTGGCCGATGGACGCCCTCCCCTGTCTCATCTCCCGCCCTCCAAGTCTCTGCTTCTCAAAATCACGCGGCGCTTTCGATTCTCCGACTACGAGAACAGCCGCAACAGCAAGAACAAATTCTCCAAAGGGTTTAAGGAGTTGGTCGCATCTTGCCTCGATCAGGAGCCGTCCAAGAGGCCCTGCGCCCAGAAATTGCTGAAGCATTCGTTCTTCAGGAATTGCAAGGGCCCCGATGTTCTGGTCAGAACTGTGCTGCAGGGTTTGCCGAGTGTTGAAGAGAGGTTCAAATTGGGGAAACTGCAGCGCTTGCCGTCGGTGAAAATGGACGGCGATGAGGGCGAGGAAGGGGAATTGGAGGAGGAGATTATCAAACAGAGGAGGATCAGCGGGTGGAATTTTAACGAGGACGGGCTGGAACTGGACCCGGTTTTCCCAGGTGAAAATTGCGAGGAGGATGAGTGTTCGATTGTAAAACAGGTTCGATTCGGCGGCGAATCGGTCATCGACGAGAAGGGTTCGGGGGATTCGAGCGAGTGCTGTTCGGGGCTGAGTTCGCCGAGCCAAACGGGGAACGACGGAGACCGGAGAAGCGGCGGCGGCGGTGGAGGGGAGGAAGAAATAGTGGACAAGGAGGCGATAGTGGGGGGATTGATGGCGTTGAAGAGGAGCTTAGACGAGCAGCGGCGGACGGTTGGGAATTTGATAGAGTTTTTGGAGGGGGAGAAAAATGGGGAGATGAACAGGGAGGAGCAAATGGTGAGGCTGATTGAGAAGATGACCAAGGAATTGGAGAATGAGAGGAACAGGAACTTCGAATTGGAGATGGAGTTGGAATTTCTAAGGCTTCAGATTTCTCCTGCATATAAT

mRNA sequence

ATCCTTGTCGCCAAAATTCAAATTCAGTCCGGTGAGGTCATCATGAATGATCAAGAAGAACCCAAAAGGCCCCAATTCCCTCTCGATTCAAGCTCTTACAGCATCATCGACGAAATTGGGGTCGGCGTGAGCGCCGTCGTGTACAAAGCCATCTGCATTCCCATGAACTCCGCGGTGGTGGCTATCAAATCCATCGACCTCGACCGATCGCGGGCCGATTTTGACGATATCCGAAGGGAAGCCCAAATTCTGTCGCTTCTCTCTCACCCCAATATTCTCAACGCCCACTGTTCTTTCACCGTCGATCGTCGGCTCTGGGTCGTCATGCCGTTTATGTCCGCCGGGTCTCTGCAGTCCATCATTACGTCGTCGTTTCCCGACGGTTTGCCGGAGCCCTGCATCGCCGCCGTACTCCGGGAAATTCTGAGCGCCCTGGCTTATCTCCACGACCAAGGCCACCTCCACCGCGACATTAAAGCCGGCAACATTCTGATGGACTCAAACGGGTCCGTTAAGCTCGCGGATTTCGGAGTTTCCACTTCGATTTTCGAGGCCAATGCGAAACAGGGGTTGTCGACTTCTTCGTTGTCGGCGTCGATGATGAAGCTAACGGACGTCGCCGGAACGCCGTACTGGATGGCGCCGGAGGTGATCCACTCGCATAATGGGTATAGTTTGAAGGCGGATATATGGTCATTTGGGATCACGGCGTTGGAATTGGCCGATGGACGCCCTCCCCTGTCTCATCTCCCGCCCTCCAAGTCTCTGCTTCTCAAAATCACGCGGCGCTTTCGATTCTCCGACTACGAGAACAGCCGCAACAGCAAGAACAAATTCTCCAAAGGGTTTAAGGAGTTGGTCGCATCTTGCCTCGATCAGGAGCCGTCCAAGAGGCCCTGCGCCCAGAAATTGCTGAAGCATTCGTTCTTCAGGAATTGCAAGGGCCCCGATGTTCTGGTCAGAACTGTGCTGCAGGGTTTGCCGAGTGTTGAAGAGAGGTTCAAATTGGGGAAACTGCAGCGCTTGCCGTCGGTGAAAATGGACGGCGATGAGGGCGAGGAAGGGGAATTGGAGGAGGAGATTATCAAACAGAGGAGGATCAGCGGGTGGAATTTTAACGAGGACGGGCTGGAACTGGACCCGGTTTTCCCAGGTGAAAATTGCGAGGAGGATGAGTGTTCGATTGTAAAACAGGTTCGATTCGGCGGCGAATCGGTCATCGACGAGAAGGGTTCGGGGGATTCGAGCGAGTGCTGTTCGGGGCTGAGTTCGCCGAGCCAAACGGGGAACGACGGAGACCGGAGAAGCGGCGGCGGCGGTGGAGGGGAGGAAGAAATAGTGGACAAGGAGGCGATAGTGGGGGGATTGATGGCGTTGAAGAGGAGCTTAGACGAGCAGCGGCGGACGGTTGGGAATTTGATAGAGTTTTTGGAGGGGGAGAAAAATGGGGAGATGAACAGGGAGGAGCAAATGGTGAGGCTGATTGAGAAGATGACCAAGGAATTGGAGAATGAGAGGAACAGGAACTTCGAATTGGAGATGGAGTTGGAATTTCTAAGGCTTCAGATTTCTCCTGCATATAAT

Coding sequence (CDS)

ATCCTTGTCGCCAAAATTCAAATTCAGTCCGGTGAGGTCATCATGAATGATCAAGAAGAACCCAAAAGGCCCCAATTCCCTCTCGATTCAAGCTCTTACAGCATCATCGACGAAATTGGGGTCGGCGTGAGCGCCGTCGTGTACAAAGCCATCTGCATTCCCATGAACTCCGCGGTGGTGGCTATCAAATCCATCGACCTCGACCGATCGCGGGCCGATTTTGACGATATCCGAAGGGAAGCCCAAATTCTGTCGCTTCTCTCTCACCCCAATATTCTCAACGCCCACTGTTCTTTCACCGTCGATCGTCGGCTCTGGGTCGTCATGCCGTTTATGTCCGCCGGGTCTCTGCAGTCCATCATTACGTCGTCGTTTCCCGACGGTTTGCCGGAGCCCTGCATCGCCGCCGTACTCCGGGAAATTCTGAGCGCCCTGGCTTATCTCCACGACCAAGGCCACCTCCACCGCGACATTAAAGCCGGCAACATTCTGATGGACTCAAACGGGTCCGTTAAGCTCGCGGATTTCGGAGTTTCCACTTCGATTTTCGAGGCCAATGCGAAACAGGGGTTGTCGACTTCTTCGTTGTCGGCGTCGATGATGAAGCTAACGGACGTCGCCGGAACGCCGTACTGGATGGCGCCGGAGGTGATCCACTCGCATAATGGGTATAGTTTGAAGGCGGATATATGGTCATTTGGGATCACGGCGTTGGAATTGGCCGATGGACGCCCTCCCCTGTCTCATCTCCCGCCCTCCAAGTCTCTGCTTCTCAAAATCACGCGGCGCTTTCGATTCTCCGACTACGAGAACAGCCGCAACAGCAAGAACAAATTCTCCAAAGGGTTTAAGGAGTTGGTCGCATCTTGCCTCGATCAGGAGCCGTCCAAGAGGCCCTGCGCCCAGAAATTGCTGAAGCATTCGTTCTTCAGGAATTGCAAGGGCCCCGATGTTCTGGTCAGAACTGTGCTGCAGGGTTTGCCGAGTGTTGAAGAGAGGTTCAAATTGGGGAAACTGCAGCGCTTGCCGTCGGTGAAAATGGACGGCGATGAGGGCGAGGAAGGGGAATTGGAGGAGGAGATTATCAAACAGAGGAGGATCAGCGGGTGGAATTTTAACGAGGACGGGCTGGAACTGGACCCGGTTTTCCCAGGTGAAAATTGCGAGGAGGATGAGTGTTCGATTGTAAAACAGGTTCGATTCGGCGGCGAATCGGTCATCGACGAGAAGGGTTCGGGGGATTCGAGCGAGTGCTGTTCGGGGCTGAGTTCGCCGAGCCAAACGGGGAACGACGGAGACCGGAGAAGCGGCGGCGGCGGTGGAGGGGAGGAAGAAATAGTGGACAAGGAGGCGATAGTGGGGGGATTGATGGCGTTGAAGAGGAGCTTAGACGAGCAGCGGCGGACGGTTGGGAATTTGATAGAGTTTTTGGAGGGGGAGAAAAATGGGGAGATGAACAGGGAGGAGCAAATGGTGAGGCTGATTGAGAAGATGACCAAGGAATTGGAGAATGAGAGGAACAGGAACTTCGAATTGGAGATGGAGTTGGAATTTCTAAGGCTTCAGATTTCTCCTGCATATAAT

Protein sequence

ILVAKIQIQSGEVIMNDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKITRRFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEIIKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGLSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEKNGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN

Homology

[Close]

BLAST of MS010365 vs. NCBI nr
Match: XP_022980836.1 (serine/threonine-protein kinase BLUS1 [Cucurbita maxima])

HSP 1 Score: 842.4 bits (2175), Expect = 2.1e-240
Identity = 438/527 (83.11%), Postives = 476/527 (90.32%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE+IM   +DQ+E KRPQFPLDSS Y IIDEIGVGVSAVVYKAICIPMNSAVVAIK
Sbjct: 11  RVQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIF
Sbjct: 131 SFPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           EAN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 EANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYENSRNS--KNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN +NS  KNKFSKGFKELVASCLDQEPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
            KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQRLPS+KM  +E +E E+E+EI
Sbjct: 311 DKLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKM--EEEDEEEIEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG
Sbjct: 371 IKQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSG 430

Query: 427 LSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 486
           +S  S    +G R   GGG  EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 431 VSDLSSPTGNGRR---GGGEAEEEAVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 490

Query: 487 NGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN 529
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFL LQIS A+N
Sbjct: 491 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLGLQISAAHN 530

[Close]

BLAST of MS010365 vs. NCBI nr
Match: XP_022924307.1 (serine/threonine-protein kinase BLUS1 [Cucurbita moschata])

HSP 1 Score: 834.7 bits (2155), Expect = 4.4e-238
Identity = 436/527 (82.73%), Postives = 475/527 (90.13%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE+IM   +DQ+E KRPQFPLDSS Y IIDEIGVGVSAVVYKAICIPMNSAVVAIK
Sbjct: 11  RVQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIF
Sbjct: 131 SFPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           EAN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 EANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYEN--SRNSKNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN  S N+KNKFSKGFKELVASCLDQEPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENQQSSNNKNKFSKGFKELVASCLDQEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
            KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQRLPS+K   +E +E E+E+EI
Sbjct: 311 DKLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKT--EEEDEEEIEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG
Sbjct: 371 IKQRRISGWNFKEDGLELEPVFPGENCEEDECSIMKQVRFGGESIM-EKGSRDSSECCSG 430

Query: 427 LSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 486
           +S  S    +G  R GG    EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 431 VSDLSSPTGNG--RRGGVEEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 490

Query: 487 NGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN 529
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEF+RLQIS A+N
Sbjct: 491 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFVRLQISAAHN 531

[Close]

BLAST of MS010365 vs. NCBI nr
Match: KAG6582364.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 834.7 bits (2155), Expect = 4.4e-238
Identity = 436/525 (83.05%), Postives = 473/525 (90.10%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE+IM   +DQ+E KRPQFPLDSS Y IIDEIGVGVSAVVYKAICIPMNSAVVAIK
Sbjct: 11  RVQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIF
Sbjct: 131 SFPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           EAN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 EANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYENSRNS--KNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN +NS  KNKFSKGFKELVASCLDQEPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
            KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQRLPS+K   +E +E E+E+EI
Sbjct: 311 DKLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKT--EEDDEEEIEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG
Sbjct: 371 IKQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSG 430

Query: 427 LSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 486
           +S  S    +G R   GG   EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 431 VSDLSSPTGNGRR---GGVEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 490

Query: 487 NGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPA 527
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFLRLQIS A
Sbjct: 491 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLRLQISAA 528

[Close]

BLAST of MS010365 vs. NCBI nr
Match: XP_023526743.1 (serine/threonine-protein kinase BLUS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.9 bits (2153), Expect = 7.5e-238
Identity = 436/527 (82.73%), Postives = 474/527 (89.94%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE+IM   +DQ+E KRPQFPLDSS Y IIDEIGVGVSAVVYKAICIPMNSAVVAIK
Sbjct: 11  RVQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIF
Sbjct: 131 SFPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           EAN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 EANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYENSRNS--KNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN +NS  KNKFSKGFKELVASCLDQEPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
            KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQRLPS+K   +E +E E+E+EI
Sbjct: 311 DKLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKT--EEEDEEEIEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG
Sbjct: 371 IKQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSG 430

Query: 427 LSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 486
           +S  S    +G  R G     EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 431 VSDLSSPTGNG--RRGEVEEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 490

Query: 487 NGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN 529
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFLRLQIS A+N
Sbjct: 491 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLRLQISAAHN 531

[Close]

BLAST of MS010365 vs. NCBI nr
Match: KAG7018775.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832.0 bits (2148), Expect = 2.8e-237
Identity = 434/525 (82.67%), Postives = 471/525 (89.71%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE+IM   +DQ+E KRPQFPLDSS Y IIDEIGVGVSAVVYKAICIPMNSAVVAIK
Sbjct: 11  RVQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIF
Sbjct: 131 SFPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           EAN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 EANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYENSRNS--KNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN +NS  KNKFSKGFKELVASCLDQEPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
            KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQRLPS+K   +E +E E+E+EI
Sbjct: 311 DKLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKT--EEDDEEEIEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG
Sbjct: 371 IKQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSG 430

Query: 427 LSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 486
           +S  S    +G R        EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 431 VSDLSSPTGNGRRGGVEEEEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 490

Query: 487 NGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPA 527
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFLRLQIS A
Sbjct: 491 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLRLQISAA 531

[Close]

BLAST of MS010365 vs. ExPASy Swiss-Prot
Match: O23304 (Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.8e-144
Identity = 279/497 (56.14%), Postives = 350/497 (70.42%), Query Frame = 0


Query: 25  QFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREAQIL 84
           +FPLD+ +Y II +IGVGVSA VYKAICIPMNS VVAIK+IDLD+SRADFD +RRE + +
Sbjct: 7   EFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLRRETKTM 66

Query: 85  SLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAAVLREILSA 144
           SLLSHPNILNA+CSFTVDR LWVVMPFMS GSL SI++SSFP GLPE CI+  L+E L+A
Sbjct: 67  SLLSHPNILNAYCSFTVDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETLNA 126

Query: 145 LAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMMKLT 204
           ++YLHDQGHLHRDIKAGNIL+DS+GSVKLADFGVS SI+E       S+S  ++S ++LT
Sbjct: 127 ISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVT----SSSGTTSSSLRLT 186

Query: 205 DVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKITRRF 264
           D+AGTPYWMAPEV+HSH GY  KADIWSFGITALELA GRPPLSHLPP KSLL+KIT+RF
Sbjct: 187 DIAGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRF 246

Query: 265 RFSDYE-----NSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVL 324
            FSDYE     +S+    KFSK F+E+V  CL+Q+P+KRP A+KLLKH FF+NCKG D +
Sbjct: 247 HFSDYEINTSGSSKKGNKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKGLDFV 306

Query: 325 VRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELE-EEIIKQRRISGWNFNEDGLE 384
           V+ VL  L + E+ F   ++     +K  GD+ EE E E EEI+K RRISGWNF ED L+
Sbjct: 307 VKNVLHSLSNAEQMFMESQI----LIKSVGDDDEEEEEEDEEIVKNRRISGWNFREDDLQ 366

Query: 385 LDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGLSSPSQTGNDGDRRSGG 444
           L PVFP    +  E            S  +E  S D  E  +   +  + G         
Sbjct: 367 LSPVFPATESDSSE-----------SSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAK 426

Query: 445 GGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEKNGEMNREEQMVRLIEKM 504
              GE    DK+ ++  L  LK+SL+ QR  V  +IE L G+K  E +REE+++ ++EK+
Sbjct: 427 NQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKE-EKSREEELLEMVEKL 483

Query: 505 TKELENERNRNFELEME 516
             ELE E+ +    + +
Sbjct: 487 KIELETEKLKTLRADKD 483

[Close]

BLAST of MS010365 vs. ExPASy Swiss-Prot
Match: Q551H4 (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fray2 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.2e-65
Identity = 135/315 (42.86%), Postives = 189/315 (60.00%), Query Frame = 0


Query: 23  RPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSR-ADFDDIRREA 82
           R  +P  +  Y + + IG G S +V +AIC+P     VAIK IDL+  +    ++IR+E 
Sbjct: 61  RKNYPNSADQYELKETIGKGGSGLVQRAICLPFQEN-VAIKIIDLEHCKNVSLEEIRKEI 120

Query: 83  QILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAAVLREI 142
           Q +SL  HPN++  H SF  +  LWV+M F+SAGS   I+  SFP G  E  IA +L+E 
Sbjct: 121 QAMSLCHHPNVVAYHTSFVYNESLWVIMDFLSAGSCSDIMRFSFPQGFEEHVIATILKEA 180

Query: 143 LSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMM 202
           L A+ Y H  G +HRDIK+GNIL+DSNG+++L+DFGVS ++        + T   S +  
Sbjct: 181 LKAICYFHKTGRIHRDIKSGNILIDSNGNIQLSDFGVSATL--------IDTGETSRNTF 240

Query: 203 KLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKIT 262
                 GTP WMAPE++   N Y    DIWSFGITALELA G+ P +  PP K LLL + 
Sbjct: 241 -----VGTPCWMAPEIMEQVN-YDYAVDIWSFGITALELARGKAPFAEYPPMKVLLLTLQ 300

Query: 263 RRFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVLVR 322
                       + ++K+S  FK+LV  CL ++PSKRP   KLL+H FF+  K PD LV+
Sbjct: 301 N----PPPSLEGDGESKWSHSFKDLVEKCLQKDPSKRPLPSKLLEHRFFKQAKKPDYLVQ 356

Query: 323 TVLQGLPSVEERFKL 337
            +L  LP + +R+++
Sbjct: 361 HILAKLPPLGQRYQM 356

[Close]

BLAST of MS010365 vs. ExPASy Swiss-Prot
Match: Q6P9R2 (Serine/threonine-protein kinase OSR1 OS=Mus musculus OX=10090 GN=Oxsr1 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 4.5e-60
Identity = 136/364 (37.36%), Postives = 200/364 (54.95%), Query Frame = 0


Query: 19  EEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 78
           E+     + ++   Y + + IG G +AVV  A C P     VAIK I+L++ +   D++ 
Sbjct: 3   EDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKER-VAIKRINLEKCQTSMDELL 62

Query: 79  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDG------LPEP 138
           +E Q +S   HPNI++ + SF V   LW+VM  +S GS+  II      G      L EP
Sbjct: 63  KEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDEP 122

Query: 139 CIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLS 198
            IA +LRE+L  L YLH  G +HRD+KAGNIL+  +GSV++ADFGVS  +         +
Sbjct: 123 TIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFL--------AT 182

Query: 199 TSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPP 258
              ++ + ++ T V GTP WMAPEV+    GY  KADIWSFGITA+ELA G  P    PP
Sbjct: 183 GGDITRNKVRKTFV-GTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPP 242

Query: 259 SKSLLLKITR-RFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFR 318
            K L+L +             +    K+ K F+++++ CL ++P KRP A +LL+H FF+
Sbjct: 243 MKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQ 302

Query: 319 NCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEIIKQRRISGWN 376
             K  + L   +LQ  P++ ER K  K++R+P     G  G   + E+         GW 
Sbjct: 303 KAKNKEFLQEKILQRAPTISERSK--KVRRVP-----GSSGRLHKTED--------GGWE 341

[Close]

BLAST of MS010365 vs. ExPASy Swiss-Prot
Match: Q863I2 (Serine/threonine-protein kinase OSR1 OS=Sus scrofa OX=9823 GN=OXSR1 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.9e-59
Identity = 135/364 (37.09%), Postives = 199/364 (54.67%), Query Frame = 0


Query: 19  EEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 78
           E+     + ++   Y + + IG G +AVV  A C P     VAIK I+L++ +   D++ 
Sbjct: 3   EDSSALPWSINKDDYELQEVIGSGATAVVQAAYCTPKKEK-VAIKRINLEKCQTSMDELL 62

Query: 79  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDG------LPEP 138
           +E Q +S   HPNI++ + SF V   LW+VM  +S GS+  II      G      L E 
Sbjct: 63  KEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDEA 122

Query: 139 CIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLS 198
            IA +LRE+L  L YLH  G +HRD+KAGNIL+  +GSV++ADFGVS  +         +
Sbjct: 123 TIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFL--------AT 182

Query: 199 TSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPP 258
              ++ + ++ T V GTP WMAPEV+    GY  KADIWSFGITA+ELA G  P    PP
Sbjct: 183 GGDITRNKVRKTFV-GTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPP 242

Query: 259 SKSLLLKITR-RFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFR 318
            K L+L +             +    K+ K F+++++ CL ++P KRP A +LL+H FF+
Sbjct: 243 MKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQ 302

Query: 319 NCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEIIKQRRISGWN 376
             K  + L   +LQ  P++ ER K  K++R+P     G  G   + E+         GW 
Sbjct: 303 KAKNKEYLQEKILQRAPTISERAK--KVRRVP-----GSSGRLHKTED--------GGWE 341

[Close]

BLAST of MS010365 vs. ExPASy Swiss-Prot
Match: Q5R495 (Serine/threonine-protein kinase OSR1 OS=Pongo abelii OX=9601 GN=OXSR1 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 3.8e-59
Identity = 135/364 (37.09%), Postives = 199/364 (54.67%), Query Frame = 0


Query: 19  EEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 78
           E+     + ++   Y + + IG G +AVV  A C P     VAIK I+L++ +   D++ 
Sbjct: 3   EDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEK-VAIKRINLEKCQTSMDELL 62

Query: 79  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDG------LPEP 138
           +E Q +S   HPNI++ + SF V   LW+VM  +S GS+  II      G      L E 
Sbjct: 63  KEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDES 122

Query: 139 CIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLS 198
            IA +LRE+L  L YLH  G +HRD+KAGNIL+  +GSV++ADFGVS  +         +
Sbjct: 123 TIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFL--------AT 182

Query: 199 TSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPP 258
              ++ + ++ T V GTP WMAPEV+    GY  KADIWSFGITA+ELA G  P    PP
Sbjct: 183 GGDITRNKVRKTFV-GTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPP 242

Query: 259 SKSLLLKITR-RFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFR 318
            K L+L +             +    K+ K F+++++ CL ++P KRP A +LL+H FF+
Sbjct: 243 MKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQ 302

Query: 319 NCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEIIKQRRISGWN 376
             K  + L   +LQ  P++ ER K  K++R+P     G  G   + E+         GW 
Sbjct: 303 KAKNKEFLQEKILQRAPTISERAK--KVRRVP-----GSSGRLHKTED--------GGWE 341

[Close]

BLAST of MS010365 vs. ExPASy TrEMBL
Match: A0A6J1IUQ1 (serine/threonine-protein kinase BLUS1 OS=Cucurbita maxima OX=3661 GN=LOC111480108 PE=4 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 1.0e-240
Identity = 438/527 (83.11%), Postives = 476/527 (90.32%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE+IM   +DQ+E KRPQFPLDSS Y IIDEIGVGVSAVVYKAICIPMNSAVVAIK
Sbjct: 11  RVQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIF
Sbjct: 131 SFPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           EAN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 EANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYENSRNS--KNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN +NS  KNKFSKGFKELVASCLDQEPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
            KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQRLPS+KM  +E +E E+E+EI
Sbjct: 311 DKLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKM--EEEDEEEIEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG
Sbjct: 371 IKQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSG 430

Query: 427 LSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 486
           +S  S    +G R   GGG  EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 431 VSDLSSPTGNGRR---GGGEAEEEAVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 490

Query: 487 NGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN 529
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFL LQIS A+N
Sbjct: 491 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLGLQISAAHN 530

[Close]

BLAST of MS010365 vs. ExPASy TrEMBL
Match: A0A6J1E8I9 (serine/threonine-protein kinase BLUS1 OS=Cucurbita moschata OX=3662 GN=LOC111431839 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.1e-238
Identity = 436/527 (82.73%), Postives = 475/527 (90.13%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE+IM   +DQ+E KRPQFPLDSS Y IIDEIGVGVSAVVYKAICIPMNSAVVAIK
Sbjct: 11  RVQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIF
Sbjct: 131 SFPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           EAN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 EANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYEN--SRNSKNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN  S N+KNKFSKGFKELVASCLDQEPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENQQSSNNKNKFSKGFKELVASCLDQEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
            KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQRLPS+K   +E +E E+E+EI
Sbjct: 311 DKLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKT--EEEDEEEIEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG
Sbjct: 371 IKQRRISGWNFKEDGLELEPVFPGENCEEDECSIMKQVRFGGESIM-EKGSRDSSECCSG 430

Query: 427 LSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 486
           +S  S    +G  R GG    EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 431 VSDLSSPTGNG--RRGGVEEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 490

Query: 487 NGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN 529
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEF+RLQIS A+N
Sbjct: 491 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFVRLQISAAHN 531

[Close]

BLAST of MS010365 vs. ExPASy TrEMBL
Match: A0A5A7U0A0 (Serine/threonine-protein kinase BLUS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001270 PE=4 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.4e-234
Identity = 430/528 (81.44%), Postives = 469/528 (88.83%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE++M   +D ++ KRPQFPLDS+SY IIDEIGVGVSAVVYKAICIPMNS VVAIK
Sbjct: 11  RVQSGELVMKLTDDHDDSKRPQFPLDSTSYKIIDEIGVGVSAVVYKAICIPMNSVVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL+YLHDQGHLHRDIKAGNIL+DSNGS+KLADFGVSTSIF
Sbjct: 131 SFPDGLPEPCIAIVLREILHALSYLHDQGHLHRDIKAGNILIDSNGSIKLADFGVSTSIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           E+N+KQG+S S LS SMM L +VAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 ESNSKQGVSISPLSTSMM-LKEVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYENSRNS--KNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN  NS  KNKFSKGFKELVASCLD EPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENHHNSKNKNKFSKGFKELVASCLDPEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
           +KLLKH FF+N KGPD+LV+T+LQGLPSVEERFKLGKLQRLPS+K   +E EE ELE+EI
Sbjct: 311 EKLLKHPFFKNSKGPDLLVKTILQGLPSVEERFKLGKLQRLPSMKT--EEEEEEELEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNFNEDGLELDPVFPGE C                S++DEKGSGDSSECCSG
Sbjct: 371 IKQRRISGWNFNEDGLELDPVFPGEEC----------------SIMDEKGSGDSSECCSG 430

Query: 427 LSS-PSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGE 486
           LS   S  GN  +R  GGGGGGEEE+VD+E+IVGGLMALK+SLDEQRR VG+LIE +EGE
Sbjct: 431 LSDLSSPVGN--ERGGGGGGGGEEEVVDRESIVGGLMALKKSLDEQRRAVGSLIELMEGE 490

Query: 487 KNGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN 529
           K GEM+REEQM RL+EKMT+ELENER RNFELEMELEFLRLQIS A+N
Sbjct: 491 KGGEMSREEQMGRLMEKMTRELENERRRNFELEMELEFLRLQISAAHN 517

[Close]

BLAST of MS010365 vs. ExPASy TrEMBL
Match: A0A1S3AXC3 (serine/threonine-protein kinase BLUS1 OS=Cucumis melo OX=3656 GN=LOC103483627 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 4.1e-234
Identity = 429/528 (81.25%), Postives = 469/528 (88.83%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSGE++M   +D ++ KRPQFPLDS+SY IIDEIGVGVSAVVYKAICIPMNS VVAIK
Sbjct: 11  RVQSGELVMKLTDDHDDSKRPQFPLDSTSYKIIDEIGVGVSAVVYKAICIPMNSVVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL+YLHDQGHLHRDIKAGNIL+DSNGS+KLADFGVSTSIF
Sbjct: 131 SFPDGLPEPCIAIVLREILHALSYLHDQGHLHRDIKAGNILIDSNGSIKLADFGVSTSIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           E+N+KQG+S S LS SMM L +VAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 ESNSKQGVSISPLSTSMM-LKEVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYENSRNS--KNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN  NS  KNKFSKGFKELVASCLD EPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENHHNSKNKNKFSKGFKELVASCLDPEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
           +KLLKH FF+N KGPD+LV+T+LQGLPSVEERFKLGKLQRLPS+K   +E EE ELE+EI
Sbjct: 311 EKLLKHPFFKNSKGPDLLVKTILQGLPSVEERFKLGKLQRLPSMKT--EEEEEEELEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNFNEDGLELDPVFPGE C                S+++EKGSGDSSECCSG
Sbjct: 371 IKQRRISGWNFNEDGLELDPVFPGEEC----------------SIMEEKGSGDSSECCSG 430

Query: 427 LSS-PSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGE 486
           LS   S  GN  +R  GGGGGGEEE+VD+E+IVGGLMALK+SLDEQRR VG+LIE +EGE
Sbjct: 431 LSDLSSPVGN--ERGGGGGGGGEEEVVDRESIVGGLMALKKSLDEQRRAVGSLIELMEGE 490

Query: 487 KNGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN 529
           K GEM+REEQM RL+EKMT+ELENER RNFELEMELEFLRLQIS A+N
Sbjct: 491 KGGEMSREEQMGRLMEKMTRELENERRRNFELEMELEFLRLQISAAHN 517

[Close]

BLAST of MS010365 vs. ExPASy TrEMBL
Match: A0A0A0L8E0 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G141880 PE=4 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 4.3e-231
Identity = 424/528 (80.30%), Postives = 468/528 (88.64%), Query Frame = 0


Query: 7   QIQSGEVIM---NDQEEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIK 66
           ++QSG++IM   +D ++ +RPQFPLDS+SY IIDEIGVGVSAVVYKAICIPMNSAVVAIK
Sbjct: 11  RVQSGDLIMKLPDDHDDSRRPQFPLDSTSYKIIDEIGVGVSAVVYKAICIPMNSAVVAIK 70

Query: 67  SIDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITS 126
           +IDLDRSRADFDDIRREA+ILSLLSHPNILN+HCSFTVDRRLWVVMPFMSAGSLQSIITS
Sbjct: 71  AIDLDRSRADFDDIRREAKILSLLSHPNILNSHCSFTVDRRLWVVMPFMSAGSLQSIITS 130

Query: 127 SFPDGLPEPCIAAVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIF 186
           SFPDGLPEPCIA VLREIL AL+YLHDQGHLHRDIKAGNIL+DSNGS+KLADFGVSTSIF
Sbjct: 131 SFPDGLPEPCIAIVLREILHALSYLHDQGHLHRDIKAGNILIDSNGSIKLADFGVSTSIF 190

Query: 187 EANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADG 246
           E+N+KQG+S S LS S+M L +VAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA G
Sbjct: 191 ESNSKQGVSISPLSTSIM-LKEVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHG 250

Query: 247 RPPLSHLPPSKSLLLKITRRFRFSDYENSRNS--KNKFSKGFKELVASCLDQEPSKRPCA 306
           RPPLSHLPPSKSLLLKITRRFRFSDYEN  NS  KNKFSKGFKELVASCLD EPS+RPCA
Sbjct: 251 RPPLSHLPPSKSLLLKITRRFRFSDYENHHNSKNKNKFSKGFKELVASCLDPEPSRRPCA 310

Query: 307 QKLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEI 366
           +KLLKH FF+N KGPD+LV+T+LQGLPSVEERFKLGKLQRLPS+K   +E EE ELE+EI
Sbjct: 311 EKLLKHPFFKNSKGPDLLVKTILQGLPSVEERFKLGKLQRLPSMKT--EEEEEEELEKEI 370

Query: 367 IKQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG 426
           IKQRRISGWNFNEDGLELDPVFPGE C                S+++EKGSG+SSECCSG
Sbjct: 371 IKQRRISGWNFNEDGLELDPVFPGEEC----------------SIMEEKGSGNSSECCSG 430

Query: 427 LSS-PSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGE 486
           LS   S  GN+    SGGGGG EEE+VD+E+IVGGLMALKRSLDEQRR VG+LIE +EGE
Sbjct: 431 LSDLSSPVGNERGGGSGGGGGEEEEVVDRESIVGGLMALKRSLDEQRRAVGSLIELMEGE 490

Query: 487 KNGEMNREEQMVRLIEKMTKELENERNRNFELEMELEFLRLQISPAYN 529
           K GEM+REEQM RL+EK+T+ELENER RNFELEMELEFLRLQIS  +N
Sbjct: 491 KVGEMSREEQMGRLMEKVTRELENERRRNFELEMELEFLRLQISAFHN 519

[Close]

BLAST of MS010365 vs. TAIR 10
Match: AT4G14480.1 (Protein kinase superfamily protein )

HSP 1 Score: 513.1 bits (1320), Expect = 2.7e-145
Identity = 279/497 (56.14%), Postives = 350/497 (70.42%), Query Frame = 0


Query: 25  QFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREAQIL 84
           +FPLD+ +Y II +IGVGVSA VYKAICIPMNS VVAIK+IDLD+SRADFD +RRE + +
Sbjct: 7   EFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLRRETKTM 66

Query: 85  SLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAAVLREILSA 144
           SLLSHPNILNA+CSFTVDR LWVVMPFMS GSL SI++SSFP GLPE CI+  L+E L+A
Sbjct: 67  SLLSHPNILNAYCSFTVDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETLNA 126

Query: 145 LAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMMKLT 204
           ++YLHDQGHLHRDIKAGNIL+DS+GSVKLADFGVS SI+E       S+S  ++S ++LT
Sbjct: 127 ISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVT----SSSGTTSSSLRLT 186

Query: 205 DVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKITRRF 264
           D+AGTPYWMAPEV+HSH GY  KADIWSFGITALELA GRPPLSHLPP KSLL+KIT+RF
Sbjct: 187 DIAGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRF 246

Query: 265 RFSDYE-----NSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVL 324
            FSDYE     +S+    KFSK F+E+V  CL+Q+P+KRP A+KLLKH FF+NCKG D +
Sbjct: 247 HFSDYEINTSGSSKKGNKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKGLDFV 306

Query: 325 VRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELE-EEIIKQRRISGWNFNEDGLE 384
           V+ VL  L + E+ F   ++     +K  GD+ EE E E EEI+K RRISGWNF ED L+
Sbjct: 307 VKNVLHSLSNAEQMFMESQI----LIKSVGDDDEEEEEEDEEIVKNRRISGWNFREDDLQ 366

Query: 385 LDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGLSSPSQTGNDGDRRSGG 444
           L PVFP    +  E            S  +E  S D  E  +   +  + G         
Sbjct: 367 LSPVFPATESDSSE-----------SSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAK 426

Query: 445 GGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEKNGEMNREEQMVRLIEKM 504
              GE    DK+ ++  L  LK+SL+ QR  V  +IE L G+K  E +REE+++ ++EK+
Sbjct: 427 NQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKE-EKSREEELLEMVEKL 483

Query: 505 TKELENERNRNFELEME 516
             ELE E+ +    + +
Sbjct: 487 KIELETEKLKTLRADKD 483

[Close]

BLAST of MS010365 vs. TAIR 10
Match: AT5G14720.1 (Protein kinase superfamily protein )

HSP 1 Score: 344.0 bits (881), Expect = 2.2e-94
Identity = 187/410 (45.61%), Postives = 252/410 (61.46%), Query Frame = 0


Query: 19  EEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 78
           E     +FPL++  Y + +EIG GVSA V++A+CIP+N  VVAIK +DL++   D D IR
Sbjct: 2   ESGSEKKFPLNAKDYKLYEEIGDGVSATVHRALCIPLN-VVVAIKVLDLEKCNNDLDGIR 61

Query: 79  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAAVL 138
           RE Q +SL++HPN+L AHCSFT   +LWVVMP+M+ GS   II SS+PDG  EP IA +L
Sbjct: 62  REVQTMSLINHPNVLQAHCSFTTGHQLWVVMPYMAGGSCLHIIKSSYPDGFEEPVIATLL 121

Query: 139 REILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSA 198
           RE L AL YLH  GH+HRD+KAGNIL+DSNG+VKLADFGVS  +F+   +Q         
Sbjct: 122 RETLKALVYLHAHGHIHRDVKAGNILLDSNGAVKLADFGVSACMFDTGDRQ--------- 181

Query: 199 SMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLL 258
                    GTP WMAPEV+   +GY  KAD+WSFGITALELA G  P S  PP K LL+
Sbjct: 182 --RSRNTFVGTPCWMAPEVMQQLHGYDFKADVWSFGITALELAHGHAPFSKYPPMKVLLM 241

Query: 259 KITRRFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDV 318
            +       DYE  +    +FSK FKE+V +CL ++P KRP ++KLLKH FF++ +  D 
Sbjct: 242 TLQNAPPGLDYERDK----RFSKAFKEMVGTCLVKDPKKRPTSEKLLKHPFFKHARPADY 301

Query: 319 LVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEIIKQRRISGWNFNEDGLE 378
           LV+T+L GLP + +R++  K +    +  +  E E    ++E I  R IS WNFN + L+
Sbjct: 302 LVKTILNGLPPLGDRYRQIKSKEADLLMQNKSEYEAHLSQQEYI--RGISAWNFNLEDLK 361

Query: 379 LDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSE-CCSGLSSPSQ 428
                      +D+ S  ++  F  +    +  S  S E   S  ++PSQ
Sbjct: 362 TQAAL----ISDDDTSHAEEPDFNQKQCERQDESALSPERASSSATAPSQ 389

[Close]

BLAST of MS010365 vs. TAIR 10
Match: AT4G10730.1 (Protein kinase superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 8.9e-88
Identity = 165/360 (45.83%), Postives = 229/360 (63.61%), Query Frame = 0


Query: 19  EEPKRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 78
           ++ K+  F ++   Y +++E+G G SAVV++AI +P N  VVAIKS+DLDR  ++ DDIR
Sbjct: 33  DKKKKKGFSVNPKDYKLMEEVGYGASAVVHRAIYLPTNE-VVAIKSLDLDRCNSNLDDIR 92

Query: 79  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAAVL 138
           REAQ ++L+ HPN++ + CSF VD  LWVVMPFM+ GS   ++ +++PDG  E  I ++L
Sbjct: 93  REAQTMTLIDHPNVIKSFCSFAVDHHLWVVMPFMAQGSCLHLMKAAYPDGFEEAAICSML 152

Query: 139 REILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSA 198
           +E L AL YLH QGH+HRD+KAGNIL+D  G +KL DFGVS  +F+   +Q    +    
Sbjct: 153 KETLKALDYLHRQGHIHRDVKAGNILLDDTGEIKLGDFGVSACLFDNGDRQRARNT---- 212

Query: 199 SMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLL 258
                    GTP WMAPEV+   +GY+ KADIWSFGITALELA G  P S  PP K LL+
Sbjct: 213 -------FVGTPCWMAPEVLQPGSGYNSKADIWSFGITALELAHGHAPFSKYPPMKVLLM 272

Query: 259 KITRRFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDV 318
            I       DY+  +    KFSK FKELVA CL ++ +KRP A+KLLKHSFF+N K P++
Sbjct: 273 TIQNAPPGLDYDRDK----KFSKSFKELVALCLVKDQTKRPTAEKLLKHSFFKNVKPPEI 332

Query: 319 LVRTVLQGLPSVEERFKLGKLQRLPSVKMDGDEGEEGELEEEIIKQRRISGWNFNEDGLE 378
            V+ +   LP +  R K  + +    + + G    + +   +   QR +S WNFN + L+
Sbjct: 333 CVKKLFVDLPPLWTRVKALQAKDAAQLALKGMASADQDAISQSEYQRGVSAWNFNIEDLK 376

[Close]

BLAST of MS010365 vs. TAIR 10
Match: AT1G79640.1 (Protein kinase superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 5.8e-87
Identity = 166/360 (46.11%), Postives = 228/360 (63.33%), Query Frame = 0


Query: 22  KRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREA 81
           ++ ++P+    Y++ + IG GVSA+V++A+CIP +  VVAIK +D +R   D ++I REA
Sbjct: 5   EKKKYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDNCDLNNISREA 64

Query: 82  QILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAAVLREI 141
           Q + L+ HPN+L +HCSF  D  LWV+MP+MS GS   I+ +++PDG  E  IA +LRE 
Sbjct: 65  QTMMLVDHPNVLKSHCSFVSDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREA 124

Query: 142 LSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMM 201
           L  L YLH  GH+HRD+KAGNIL+ + G+VKL DFGVS  +F++  +Q    +       
Sbjct: 125 LKGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNT------- 184

Query: 202 KLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKIT 261
                 GTP WMAPEV+   +GY  KADIWSFGIT LELA G  P S  PP K LL+ + 
Sbjct: 185 ----FVGTPCWMAPEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQ 244

Query: 262 RRFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVLVR 321
                 DYE  +    KFS+ FK+++ASCL ++PSKRP A+KLLKHSFF+  +  D + R
Sbjct: 245 NAPPGLDYERDK----KFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIAR 304

Query: 322 TVLQGLPSVEERFKLGKLQR---LPSVKMDGDEGEEGELEEEIIKQRRISGWNFNEDGLE 379
            +L GLP +  R +  K +    L   KM   +GE+ EL +   K R ISGWNFN D ++
Sbjct: 305 KLLDGLPDLVNRVQAIKRKEEDMLAQEKM--ADGEKEELSQNEYK-RGISGWNFNLDDMK 345

[Close]

BLAST of MS010365 vs. TAIR 10
Match: AT1G79640.2 (Protein kinase superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 5.8e-87
Identity = 166/360 (46.11%), Postives = 228/360 (63.33%), Query Frame = 0


Query: 22  KRPQFPLDSSSYSIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREA 81
           ++ ++P+    Y++ + IG GVSA+V++A+CIP +  VVAIK +D +R   D ++I REA
Sbjct: 2   EKKKYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDNCDLNNISREA 61

Query: 82  QILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAAVLREI 141
           Q + L+ HPN+L +HCSF  D  LWV+MP+MS GS   I+ +++PDG  E  IA +LRE 
Sbjct: 62  QTMMLVDHPNVLKSHCSFVSDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREA 121

Query: 142 LSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMM 201
           L  L YLH  GH+HRD+KAGNIL+ + G+VKL DFGVS  +F++  +Q    +       
Sbjct: 122 LKGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNT------- 181

Query: 202 KLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKIT 261
                 GTP WMAPEV+   +GY  KADIWSFGIT LELA G  P S  PP K LL+ + 
Sbjct: 182 ----FVGTPCWMAPEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQ 241

Query: 262 RRFRFSDYENSRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVLVR 321
                 DYE  +    KFS+ FK+++ASCL ++PSKRP A+KLLKHSFF+  +  D + R
Sbjct: 242 NAPPGLDYERDK----KFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIAR 301

Query: 322 TVLQGLPSVEERFKLGKLQR---LPSVKMDGDEGEEGELEEEIIKQRRISGWNFNEDGLE 379
            +L GLP +  R +  K +    L   KM   +GE+ EL +   K R ISGWNFN D ++
Sbjct: 302 KLLDGLPDLVNRVQAIKRKEEDMLAQEKM--ADGEKEELSQNEYK-RGISGWNFNLDDMK 342

The following BLAST results are available for this feature:

BLAST of MS010365 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022980836.1	2.1e-240	83.11	serine/threonine-protein kinase BLUS1 [Cucurbita maxima]	[more]
XP_022924307.1	4.4e-238	82.73	serine/threonine-protein kinase BLUS1 [Cucurbita moschata]	[more]
KAG6582364.1	4.4e-238	83.05	Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023526743.1	7.5e-238	82.73	serine/threonine-protein kinase BLUS1 [Cucurbita pepo subsp. pepo]	[more]
KAG7018775.1	2.8e-237	82.67	Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. ar...	[more]

BLAST of MS010365 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23304	3.8e-144	56.14	Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 P...	[more]
Q551H4	1.2e-65	42.86	Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fr...	[more]
Q6P9R2	4.5e-60	37.36	Serine/threonine-protein kinase OSR1 OS=Mus musculus OX=10090 GN=Oxsr1 PE=1 SV=1	[more]
Q863I2	2.9e-59	37.09	Serine/threonine-protein kinase OSR1 OS=Sus scrofa OX=9823 GN=OXSR1 PE=2 SV=1	[more]
Q5R495	3.8e-59	37.09	Serine/threonine-protein kinase OSR1 OS=Pongo abelii OX=9601 GN=OXSR1 PE=2 SV=1	[more]

BLAST of MS010365 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IUQ1	1.0e-240	83.11	serine/threonine-protein kinase BLUS1 OS=Cucurbita maxima OX=3661 GN=LOC11148010...	[more]
A0A6J1E8I9	2.1e-238	82.73	serine/threonine-protein kinase BLUS1 OS=Cucurbita moschata OX=3662 GN=LOC111431...	[more]
A0A5A7U0A0	1.4e-234	81.44	Serine/threonine-protein kinase BLUS1 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3AXC3	4.1e-234	81.25	serine/threonine-protein kinase BLUS1 OS=Cucumis melo OX=3656 GN=LOC103483627 PE...	[more]
A0A0A0L8E0	4.3e-231	80.30	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G141...	[more]

BLAST of MS010365 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14480.1	2.7e-145	56.14	Protein kinase superfamily protein	[more]
AT5G14720.1	2.2e-94	45.61	Protein kinase superfamily protein	[more]
AT4G10730.1	8.9e-88	45.83	Protein kinase superfamily protein	[more]
AT1G79640.1	5.8e-87	46.11	Protein kinase superfamily protein	[more]
AT1G79640.2	5.8e-87	46.11	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 485..522
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 115..332 e-value: 4.3E-54 score: 184.9
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 22..111 e-value: 3.3E-28 score: 99.6
None	No IPR available	PIRSR	PIRSR037014-1	PIRSR037014-1	coord: 13..241 e-value: 8.5E-22 score: 75.1
None	No IPR available	PIRSR	PIRSR000556-1	PIRSR000556-1	coord: 28..301 e-value: 1.2E-21 score: 74.7
None	No IPR available	PIRSR	PIRSR037014-2	PIRSR037014-2	coord: 13..241 e-value: 8.5E-22 score: 75.1
None	No IPR available	PIRSR	PIRSR000620-2	PIRSR000620-2	coord: 31..306 e-value: 1.2E-15 score: 55.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 406..446
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 416..433
None	No IPR available	PANTHER	PTHR48014:SF7	SERINE/THREONINE-PROTEIN KINASE BLUS1	coord: 16..523
None	No IPR available	PANTHER	PTHR48014	SERINE/THREONINE-PROTEIN KINASE FRAY2	coord: 16..523
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 33..310 e-value: 1.4E-85 score: 300.3
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 33..310 e-value: 2.2E-58 score: 197.7
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 33..310 score: 47.664806
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 39..63
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 31..327

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010365.1	MS010365.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity