MS028533 (gene) Bitter gourd (TR) v1

Overview


Name	MS028533
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	(R)-mandelonitrile lyase
Location	scaffold29: 263537 .. 264484 (-)
RNA-Seq Expression	MS028533
Synteny	MS028533

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGCGTGAAAAGGGAGAGGTGATGCTCTGTGCTGGGGCTCTCGGAAGCCCACAGCTTCTGCTTCTGAGTGGCATTGGACCGAGGCCGTCCCTCTCATCGTGGGGAATTCCTGTGTCTCATCACTTGCCTTATGTCGGACAGTTCCTCTATGATAACCCCCGAAATGGCATCTCAATTGTGCCCCCAATGCCATTGGAGCACTCTCTGATACAAGTCGTGGGCATAACCAATTCAGGGGCAGGGGCTTACCTTGAAGCAACTTCCAATGTCATACCTTTTGTCTCCCCTGCTCGCTCTGTTTTCATTCGGAACCCAGCTTCACCACTGTATCTCACTGTGGCCACCCTGATGGAGAAGATTACTGGGCCACTCTCCGCAGGTTCACTCCGGTTGCCATCTACGGATGTCAGGGTTAACCCAATTGTCCGGTTCAACTACTTTAGTAATCCGGGTGATGTGGAGCGGTGTGTGAATGGCACCCGCAAGATCGGTGATGTACTGAGGAGCCGTTCCATGGGGGATTTCCAATTCAGGGAATGGTTTGGGGGCCGAGATTGAAGATACGTGGGGCCTGCATTGCCCGTTGATCAATCAAATGATCGGCTGATGGGAGAGTTTTGCCGTCGCACAGTGAGTACTATATGGCACTACCATGGTGGATGCCTTGTGGGCAAGGTAGTTGATCGCAATTTCAGGGTTATTGGGATTGATGCTCTCCGAGTTGTTGATGGTTCGATATTCACTGTATCACCAGGGACCAATCCCCAGTCCCCTCTTTTGATGCTTGGACGGTCAGTGTGTTTGTTGTTATCTGAATGCTTGAGTGTTTTTGTTGGTTTGTTTTGGCAGGTATGTTGGTATGAAGATATTCAGAGAGAGAATGATCTCGAGTTGACCACTTACCCTGTTGTGCCAAAAGGAACTATAAGCGGACTTAGCTGCTAA

mRNA sequence

ATGGTGCGTGAAAAGGGAGAGGTGATGCTCTGTGCTGGGGCTCTCGGAAGCCCACAGCTTCTGCTTCTGAGTGGCATTGGACCGAGGCCGTCCCTCTCATCGTGGGGAATTCCTGTGTCTCATCACTTGCCTTATGTCGGACAGTTCCTCTATGATAACCCCCGAAATGGCATCTCAATTGTGCCCCCAATGCCATTGGAGCACTCTCTGATACAAGTCGTGGGCATAACCAATTCAGGGGCAGGGGCTTACCTTGAAGCAACTTCCAATGTCATACCTTTTGTCTCCCCTGCTCGCTCTGTATGTTGGTATGAAGATATTCAGAGAGAGAATGATCTCGAGTTGACCACTTACCCTGTTGTGCCAAAAGGAACTATAAGCGGACTTAGCTGCTAA

Coding sequence (CDS)

ATGGTGCGTGAAAAGGGAGAGGTGATGCTCTGTGCTGGGGCTCTCGGAAGCCCACAGCTTCTGCTTCTGAGTGGCATTGGACCGAGGCCGTCCCTCTCATCGTGGGGAATTCCTGTGTCTCATCACTTGCCTTATGTCGGACAGTTCCTCTATGATAACCCCCGAAATGGCATCTCAATTGTGCCCCCAATGCCATTGGAGCACTCTCTGATACAAGTCGTGGGCATAACCAATTCAGGGGCAGGGGCTTACCTTGAAGCAACTTCCAATGTCATACCTTTTGTCTCCCCTGCTCGCTCTGTATGTTGGTATGAAGATATTCAGAGAGAGAATGATCTCGAGTTGACCACTTACCCTGTTGTGCCAAAAGGAACTATAAGCGGACTTAGCTGCTAA

Protein sequence

MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSVCWYEDIQRENDLELTTYPVVPKGTISGLSC

Homology

[Close]

BLAST of MS028533 vs. NCBI nr
Match: KAF4392710.1 (hypothetical protein G4B88_029449 [Cannabis sativa])

HSP 1 Score: 192.2 bits (487), Expect = 2.8e-45
Identity = 95/101 (94.06%), Postives = 96/101 (95.05%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEVMLCAGALGSPQLLLLSGIGPRP LSSWGIPV+HHLPYVGQFLYDNPRNGISI
Sbjct: 198 MVREKGEVMLCAGALGSPQLLLLSGIGPRPYLSSWGIPVAHHLPYVGQFLYDNPRNGISI 257

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPPMPLEHSLIQVVGITNS  GAYLEA SNVIPF SPARSV
Sbjct: 258 VPPMPLEHSLIQVVGITNS--GAYLEAASNVIPFASPARSV 296

[Close]

BLAST of MS028533 vs. NCBI nr
Match: KAF4349905.1 (hypothetical protein G4B88_002327 [Cannabis sativa])

HSP 1 Score: 192.2 bits (487), Expect = 2.8e-45
Identity = 95/101 (94.06%), Postives = 96/101 (95.05%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEVMLCAGALGSPQLLLLSGIGPRP LSSWGIPV+HHLPYVGQFLYDNPRNGISI
Sbjct: 571 MVREKGEVMLCAGALGSPQLLLLSGIGPRPYLSSWGIPVAHHLPYVGQFLYDNPRNGISI 630

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPPMPLEHSLIQVVGITNS  GAYLEA SNVIPF SPARSV
Sbjct: 631 VPPMPLEHSLIQVVGITNS--GAYLEAASNVIPFASPARSV 669

[Close]

BLAST of MS028533 vs. NCBI nr
Match: THG00223.1 (hypothetical protein TEA_020656 [Camellia sinensis var. sinensis])

HSP 1 Score: 184.9 bits (468), Expect = 4.5e-43
Identity = 90/101 (89.11%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEV+LCAGALGSPQLLLLSGIGPRP LSSWGIPV+HH PYVGQFLYDNPRNGISI
Sbjct: 264 MVREKGEVLLCAGALGSPQLLLLSGIGPRPYLSSWGIPVAHHSPYVGQFLYDNPRNGISI 323

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPP PLEHSLIQVVGITNS  GAYLEA SN++PF SPARSV
Sbjct: 324 VPPTPLEHSLIQVVGITNS--GAYLEAASNIVPFSSPARSV 362

[Close]

BLAST of MS028533 vs. NCBI nr
Match: XP_028113899.1 ((R)-mandelonitrile lyase-like [Camellia sinensis])

HSP 1 Score: 184.9 bits (468), Expect = 4.5e-43
Identity = 90/101 (89.11%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEV+LCAGALGSPQLLLLSGIGPRP LSSWGIPV+HH PYVGQFLYDNPRNGISI
Sbjct: 276 MVREKGEVLLCAGALGSPQLLLLSGIGPRPYLSSWGIPVAHHSPYVGQFLYDNPRNGISI 335

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPP PLEHSLIQVVGITNS  GAYLEA SN++PF SPARSV
Sbjct: 336 VPPTPLEHSLIQVVGITNS--GAYLEAASNIVPFSSPARSV 374

[Close]

BLAST of MS028533 vs. NCBI nr
Match: PSS07265.1 ((R)-mandelonitrile lyase-like [Actinidia chinensis var. chinensis])

HSP 1 Score: 181.4 bits (459), Expect = 5.0e-42
Identity = 87/101 (86.14%), Postives = 93/101 (92.08%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEV+LCAGA+GSPQLLLLSGIGPRP LSSWGIPV HHLPYVGQFLYDNPRNGIS 
Sbjct: 274 MVREKGEVILCAGAIGSPQLLLLSGIGPRPYLSSWGIPVVHHLPYVGQFLYDNPRNGISF 333

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPP+PLEHSLIQVVGIT+S  GAYLEA SN++PF SP RSV
Sbjct: 334 VPPIPLEHSLIQVVGITDS--GAYLEAASNIVPFASPVRSV 372

[Close]

BLAST of MS028533 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.0e-34
Identity = 71/101 (70.30%), Postives = 85/101 (84.16%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           ++R++GEV+L AGALGSPQLL LSGIGPR  LS+WGIPV+   P+VG F+YDNPRNGISI
Sbjct: 278 LIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISI 337

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPP+P+E+SLIQVVG+T    GA+LEA SNVIPF SP  SV
Sbjct: 338 VPPVPMENSLIQVVGVTED--GAFLEAASNVIPFASPLHSV 376

[Close]

BLAST of MS028533 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 7.8e-22
Identity = 50/96 (52.08%), Postives = 70/96 (72.92%), Query Frame = 0


Query: 2   VREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISIV 61
           VR KGEV++ AG +G+PQLLLLSG+GP   LSS  IPV    PYVGQFL+DNPRN I+I+
Sbjct: 274 VRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINIL 333

Query: 62  PPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSP 98
           PP P+E +++ V+GI+N     + + + + +PF +P
Sbjct: 334 PPNPIEPTIVTVLGISND----FYQCSFSSLPFTTP 365

[Close]

BLAST of MS028533 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 7.8e-22
Identity = 50/96 (52.08%), Postives = 70/96 (72.92%), Query Frame = 0


Query: 2   VREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISIV 61
           VR KGEV++ AG +G+PQLLLLSG+GP   LSS  IPV    PYVGQFL+DNPRN I+I+
Sbjct: 274 VRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINIL 333

Query: 62  PPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSP 98
           PP P+E +++ V+GI+N     + + + + +PF +P
Sbjct: 334 PPNPIEPTIVTVLGISND----FYQCSFSSLPFTTP 365

[Close]

BLAST of MS028533 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.3e-20
Identity = 49/96 (51.04%), Postives = 68/96 (70.83%), Query Frame = 0


Query: 2   VREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISIV 61
           VR +GEV+L AG +GSPQLLLLSG+GP   L+S  I V    PYVGQ++YDNPRN I+I+
Sbjct: 275 VRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINIL 334

Query: 62  PPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSP 98
           PP P+E S + V+GIT+     + + + + +PF +P
Sbjct: 335 PPNPIEASTVTVLGITSD----FYQCSISSLPFDTP 366

[Close]

BLAST of MS028533 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 3.6e-19
Identity = 49/100 (49.00%), Postives = 67/100 (67.00%), Query Frame = 0


Query: 2   VREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISIV 61
           VR  GEV++ AG +G+PQLLLLSG+GP   LSS  I V    PYVGQFLY+NPRN I+  
Sbjct: 275 VRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQPNPYVGQFLYNNPRNFINNF 334

Query: 62  PPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           PP P+E S++ V+GI +     Y + + + +PF +P  S+
Sbjct: 335 PPNPIEASVVTVLGIRSD----YYQVSLSSLPFSTPPFSL 370

[Close]

BLAST of MS028533 vs. ExPASy TrEMBL
Match: A0A7J6DUX9 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_002327 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.4e-45
Identity = 95/101 (94.06%), Postives = 96/101 (95.05%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEVMLCAGALGSPQLLLLSGIGPRP LSSWGIPV+HHLPYVGQFLYDNPRNGISI
Sbjct: 571 MVREKGEVMLCAGALGSPQLLLLSGIGPRPYLSSWGIPVAHHLPYVGQFLYDNPRNGISI 630

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPPMPLEHSLIQVVGITNS  GAYLEA SNVIPF SPARSV
Sbjct: 631 VPPMPLEHSLIQVVGITNS--GAYLEAASNVIPFASPARSV 669

[Close]

BLAST of MS028533 vs. ExPASy TrEMBL
Match: A0A7J6HEB8 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_029449 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.4e-45
Identity = 95/101 (94.06%), Postives = 96/101 (95.05%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEVMLCAGALGSPQLLLLSGIGPRP LSSWGIPV+HHLPYVGQFLYDNPRNGISI
Sbjct: 198 MVREKGEVMLCAGALGSPQLLLLSGIGPRPYLSSWGIPVAHHLPYVGQFLYDNPRNGISI 257

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPPMPLEHSLIQVVGITNS  GAYLEA SNVIPF SPARSV
Sbjct: 258 VPPMPLEHSLIQVVGITNS--GAYLEAASNVIPFASPARSV 296

[Close]

BLAST of MS028533 vs. ExPASy TrEMBL
Match: A0A4S4DCA1 (GMC_OxRdtase_N domain-containing protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_020656 PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.2e-43
Identity = 90/101 (89.11%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEV+LCAGALGSPQLLLLSGIGPRP LSSWGIPV+HH PYVGQFLYDNPRNGISI
Sbjct: 264 MVREKGEVLLCAGALGSPQLLLLSGIGPRPYLSSWGIPVAHHSPYVGQFLYDNPRNGISI 323

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPP PLEHSLIQVVGITNS  GAYLEA SN++PF SPARSV
Sbjct: 324 VPPTPLEHSLIQVVGITNS--GAYLEAASNIVPFSSPARSV 362

[Close]

BLAST of MS028533 vs. ExPASy TrEMBL
Match: A0A2R6QF72 ((R)-mandelonitrile lyase-like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc17603 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.4e-42
Identity = 87/101 (86.14%), Postives = 93/101 (92.08%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEV+LCAGA+GSPQLLLLSGIGPRP LSSWGIPV HHLPYVGQFLYDNPRNGIS 
Sbjct: 1   MVREKGEVILCAGAIGSPQLLLLSGIGPRPYLSSWGIPVVHHLPYVGQFLYDNPRNGISF 60

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPP+PLEHSLIQVVGIT+S  GAYLEA SN++PF SP RSV
Sbjct: 61  VPPIPLEHSLIQVVGITDS--GAYLEAASNIVPFASPVRSV 99

[Close]

BLAST of MS028533 vs. ExPASy TrEMBL
Match: A0A2R6QF71 ((R)-mandelonitrile lyase-like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc17604 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.4e-42
Identity = 87/101 (86.14%), Postives = 93/101 (92.08%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           MVREKGEV+LCAGA+GSPQLLLLSGIGPRP LSSWGIPV HHLPYVGQFLYDNPRNGIS 
Sbjct: 274 MVREKGEVILCAGAIGSPQLLLLSGIGPRPYLSSWGIPVVHHLPYVGQFLYDNPRNGISF 333

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPP+PLEHSLIQVVGIT+S  GAYLEA SN++PF SP RSV
Sbjct: 334 VPPIPLEHSLIQVVGITDS--GAYLEAASNIVPFASPVRSV 372

[Close]

BLAST of MS028533 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 147.5 bits (371), Expect = 7.4e-36
Identity = 71/101 (70.30%), Postives = 85/101 (84.16%), Query Frame = 0


Query: 1   MVREKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISI 60
           ++R++GEV+L AGALGSPQLL LSGIGPR  LS+WGIPV+   P+VG F+YDNPRNGISI
Sbjct: 278 LIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISI 337

Query: 61  VPPMPLEHSLIQVVGITNSGAGAYLEATSNVIPFVSPARSV 102
           VPP+P+E+SLIQVVG+T    GA+LEA SNVIPF SP  SV
Sbjct: 338 VPPVPMENSLIQVVGVTED--GAFLEAASNVIPFASPLHSV 376

[Close]

BLAST of MS028533 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 87.8 bits (216), Expect = 7.0e-18
Identity = 44/81 (54.32%), Postives = 57/81 (70.37%), Query Frame = 0


Query: 7   EVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISIVPPMPL 66
           E++L AG LGSPQLL+LSG+GP   L +  I V    P+VGQ +YDNP N + +  P+P+
Sbjct: 272 EIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPV 331

Query: 67  EHSLIQVVGITNSGAGAYLEA 88
           E SLI+VVGIT  G G Y+EA
Sbjct: 332 EVSLIEVVGIT--GEGTYVEA 350

[Close]

BLAST of MS028533 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 82.8 bits (203), Expect = 2.2e-16
Identity = 49/87 (56.32%), Postives = 59/87 (67.82%), Query Frame = 0


Query: 7   EVMLCAGALGSPQLLLLSGIGPRPSLSSWGI-PVSHHLPYVGQFLYDNPRNGISIVPPMP 66
           EV+L AGA+GSPQLL+LSGIGP   L++ GI P+    P VGQ + DNP N I I  P P
Sbjct: 272 EVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTP 331

Query: 67  LEHSLIQVVGITNSGAGAYLEATSNVI 93
           +E SLIQVVGIT     +Y+E  S VI
Sbjct: 332 VEVSLIQVVGITK--FESYIEGASGVI 356

[Close]

BLAST of MS028533 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 82.8 bits (203), Expect = 2.2e-16
Identity = 49/87 (56.32%), Postives = 59/87 (67.82%), Query Frame = 0


Query: 7   EVMLCAGALGSPQLLLLSGIGPRPSLSSWGI-PVSHHLPYVGQFLYDNPRNGISIVPPMP 66
           EV+L AGA+GSPQLL+LSGIGP   L++ GI P+    P VGQ + DNP N I I  P P
Sbjct: 239 EVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTP 298

Query: 67  LEHSLIQVVGITNSGAGAYLEATSNVI 93
           +E SLIQVVGIT     +Y+E  S VI
Sbjct: 299 VEVSLIQVVGITK--FESYIEGASGVI 323

[Close]

BLAST of MS028533 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 79.3 bits (194), Expect = 2.5e-15
Identity = 42/87 (48.28%), Postives = 57/87 (65.52%), Query Frame = 0


Query: 3   REKGEVMLCAGALGSPQLLLLSGIGPRPSLSSWGIPVSHHLPYVGQFLYDNPRNGISIVP 62
           R+  EV+L +GA+GSPQ+L+LSGIGP+  L    IPV     +VG+ + DNP N I +  
Sbjct: 284 RKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPS 343

Query: 63  PMPLEHSLIQVVGITNSGAGAYLEATS 90
             P+E SLIQ VGIT    G Y+EA++
Sbjct: 344 KAPIEQSLIQTVGITK--MGVYVEAST 368

The following BLAST results are available for this feature:

BLAST of MS028533 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF4392710.1	2.8e-45	94.06	hypothetical protein G4B88_029449 [Cannabis sativa]	[more]
KAF4349905.1	2.8e-45	94.06	hypothetical protein G4B88_002327 [Cannabis sativa]	[more]
THG00223.1	4.5e-43	89.11	hypothetical protein TEA_020656 [Camellia sinensis var. sinensis]	[more]
XP_028113899.1	4.5e-43	89.11	(R)-mandelonitrile lyase-like [Camellia sinensis]	[more]
PSS07265.1	5.0e-42	86.14	(R)-mandelonitrile lyase-like [Actinidia chinensis var. chinensis]	[more]

BLAST of MS028533 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSM2	1.0e-34	70.30	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
P52706	7.8e-22	52.08	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	7.8e-22	52.08	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
P52707	4.3e-20	51.04	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
O24243	3.6e-19	49.00	(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1	[more]

BLAST of MS028533 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7J6DUX9	1.4e-45	94.06	(R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_002327 PE=4 SV=1	[more]
A0A7J6HEB8	1.4e-45	94.06	(R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_029449 PE=4 SV=1	[more]
A0A4S4DCA1	2.2e-43	89.11	GMC_OxRdtase_N domain-containing protein OS=Camellia sinensis var. sinensis OX=5...	[more]
A0A2R6QF72	2.4e-42	86.14	(R)-mandelonitrile lyase-like OS=Actinidia chinensis var. chinensis OX=1590841 G...	[more]
A0A2R6QF71	2.4e-42	86.14	(R)-mandelonitrile lyase-like OS=Actinidia chinensis var. chinensis OX=1590841 G...	[more]

BLAST of MS028533 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	7.4e-36	70.30	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	7.0e-18	54.32	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	2.2e-16	56.32	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.2	2.2e-16	56.32	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	2.5e-15	48.28	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 1..82 e-value: 5.6E-21 score: 76.9
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 6..74
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 2..54 e-value: 2.7E-12 score: 46.7
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 13..27
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 1..103
None	No IPR available	PANTHER	PTHR45968:SF2	(R)-MANDELONITRILE LYASE-LIKE	coord: 1..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS028533.1	MS028533.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors