Tan0015600 (gene) Snake gourd v1

BLAST of Tan0015600 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 560/761 (73.59%), Postives = 659/761 (86.60%), Query Frame = 0

Query: 46  LLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAESPKILHVYDTVFHG 105
           LL L F   + +A    KTFIFR+D  S PS+FPTHYHWY++EFAE  +I+HVY TVFHG
Sbjct: 9   LLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTVFHG 68

Query: 106 FSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIG 165
           FSA +T ++ D++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGSDVIIG
Sbjct: 69  FSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIG 128

Query: 166 VFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGSNA 225
           VFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG +     
Sbjct: 129 VFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQ----- 188

Query: 226 AGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVY 285
           A  I GIN T+E+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPKAR+A Y
Sbjct: 189 AAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAY 248

Query: 286 KVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGV 345
           KVCWK+SGC DSDILAAFDAAV DGVDVISISIGGGDG++SPYYLDPIAIG+YGAAS+G+
Sbjct: 249 KVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGI 308

Query: 346 FVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYGVSLYAGAPLNG 405
           FVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA   LG+G ++ GVSLYAG PLNG
Sbjct: 309 FVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNG 368

Query: 406 TMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKGLVVKKAGAVGMIL 465
            M+P+VYPGKSG+ S SLCMEN+LDPK V GKIVICDRGSSPRVAKGLVVKKAG VGMIL
Sbjct: 369 RMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMIL 428

Query: 466 ANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAPVVA 525
           ANG SNGEGLVGDAH++PACAVGS+EGD +KAYASS  NP A+I F+GTI+GIKPAPV+A
Sbjct: 429 ANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIA 488

Query: 526 SFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPH 585
           SFS RGPNGL+PEILKPDLIAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTSMACPH
Sbjct: 489 SFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPH 548

Query: 586 VSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTAYDFGAGHVNLG 645
           VSGAAALLKSAHPDWSPA +RSAMMTT ++ DN  + + +ESTGK++T YD+G+GH+NLG
Sbjct: 549 VSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLG 608

Query: 646 LAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCP-AKKPLPENLNYPSIVAVF 705
            AM+PGLVYDITN DY+ FLCSIGYG K IQVITRTPV CP  +KP P NLNYPSI AVF
Sbjct: 609 RAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVF 668

Query: 706 SSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVFSTAVKKQSFVVAI 765
            +  +G  +K+ IRTATNVG   +VYRA+IE+P+GVTV VKP +LVF++AVK++S+ V +
Sbjct: 669 PTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTV 728

Query: 766 SADNQNLALGDVGAVFGWLSWSD-GKHVVRSPLVVTQLEPL 805
           + + +N+ LG+ GAVFG ++W D GKHVVRSP+VVTQ++ L
Sbjct: 729 TVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of Tan0015600 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 1.4e-246
Identity = 450/780 (57.69%), Postives = 548/780 (70.26%), Query Frame = 0

Query: 43  FFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA----ESPKILHV 102
           +FF LL L   S+ ++ S   T+I  VD  +KPS+FPTH+HWYTS  A      P I+H 
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHT 65

Query: 103 YDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDS 162
           YDTVFHGFSA LT +    +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +S
Sbjct: 66  YDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEES 125

Query: 163 DYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFS 222
           D+GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF 
Sbjct: 126 DFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFC 185

Query: 223 KGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGV 282
            G+EA +        +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+
Sbjct: 186 GGYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 245

Query: 283 APKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIG 342
           APKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD IAIG
Sbjct: 246 APKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIG 305

Query: 343 AYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYGVS 402
           A+GA  RG+FVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ I GVS
Sbjct: 306 AFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVS 365

Query: 403 LYAGAPLN-GTMYPLVYPGK---SGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKG 462
           +Y G  L+ G MYPLVY G        S SLC+E SLDP LV GKIV+CDRG + R  KG
Sbjct: 366 VYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKG 425

Query: 463 LVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYAS------SSANPT 522
            +V+K G +GMI+ANG+ +GEGLV D HVLPA +VG+  GD ++ Y S      SS +PT
Sbjct: 426 EIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT 485

Query: 523 ATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDF 582
           ATI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  
Sbjct: 486 ATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTS 545

Query: 583 DTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEE 642
           D R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA   DN  +PM +E
Sbjct: 546 DNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDE 605

Query: 643 STGKASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCP 702
           STG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  ITR    C 
Sbjct: 606 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 665

Query: 703 AKKPLPE--NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKV 762
             +      NLNYPS   VF    +   +  FIRT TNVG ++SVY  KI  P+G TV V
Sbjct: 666 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 725

Query: 763 KPSKLVFSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           +P KL F    +K SFVV +      L+ G      G + WSDGK  V SPLVVT  +PL
Sbjct: 726 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of Tan0015600 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 8.8e-209
Identity = 395/776 (50.90%), Postives = 525/776 (67.65%), Query Frame = 0

Query: 31  MAACFATSLAPLFFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTS--- 90
           M++ F +S A   FFLLL L      S+ S   T+I  + +   PS F  H +WY S   
Sbjct: 1   MSSSFLSSTA---FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLR 60

Query: 91  EFAESPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL- 150
             ++S ++L+ Y+   HGFS  LTQE+ DS+   P V++V  + R +LHTTR+P FLGL 
Sbjct: 61  SISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 120

Query: 151 RNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCN 210
            +   L+ ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CN
Sbjct: 121 EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 180

Query: 211 RKIVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLE 270
           RK++GARFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL 
Sbjct: 181 RKLIGARFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL 240

Query: 271 GYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSS 330
           GYASG A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S
Sbjct: 241 GYASGTARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MS 300

Query: 331 PYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTL 390
            YY D +AIGA+ A  RG+ VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+  L
Sbjct: 301 DYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAIL 360

Query: 391 GNGRKIYGVSLYAGAPLNGTMYPLVYPGK-SGVLSVSLCMENSLDPKLVSGKIVICDRGS 450
           GNG+   GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG 
Sbjct: 361 GNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGI 420

Query: 451 SPRVAKGLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANP 510
           + RV KG VVK AG VGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NP
Sbjct: 421 NARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 480

Query: 511 TATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLD 570
           TA+I+  GT++G+KP+PVVA+FS+RGPN + P ILKPDLIAPGVNILAAWT A GPTGL 
Sbjct: 481 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLA 540

Query: 571 FDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTE 630
            D+R+ EFNI+SGTSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T    +P+ +
Sbjct: 541 SDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLD 600

Query: 631 ESTGKASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTC 690
            +TGK ST +D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y +  I+ ++R   TC
Sbjct: 601 IATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTC 660

Query: 691 -PAKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEA-PKGVTVK 750
            P+K     +LNYPS    F+    G     + RT T+VG   + Y  K+ +   GV + 
Sbjct: 661 DPSKSYSVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKIS 720

Query: 751 VKPSKLVFSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVT 800
           V+P+ L F  A +K+S+ V  + D+   +  +    FG + WSDGKHVV SP+ ++
Sbjct: 721 VEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of Tan0015600 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 1.1e-203
Identity = 377/763 (49.41%), Postives = 505/763 (66.19%), Query Frame = 0

Query: 36  ATSLAPLFFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAESPKI 95
           ++S+  +  FL LLL         +  KT+I RV+   KP  F TH+ WYTS+      +
Sbjct: 8   SSSITIITTFLFLLLH-------TTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSESSL 67

Query: 96  LHVYDTVFHGFSATLTQEQVDS-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 155
           L+ Y T FHGFSA L   + DS +    S+L +FED    LHTTR+P+FLGL ++ G+  
Sbjct: 68  LYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHD 127

Query: 156 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARF 215
                + VIIGV DTG+ PE RSF D ++  IP +WKG C++G+ F +K CN+K++GAR 
Sbjct: 128 LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARS 187

Query: 216 FSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAK 275
           FSKG +  S       G +   E  SPRD DGHGTHT++TAAG     AS  GYA+G A+
Sbjct: 188 FSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTAR 247

Query: 276 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 335
           G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D IA
Sbjct: 248 GMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRDTIA 307

Query: 336 IGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYG 395
           IGA+ A  RGVFVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   LGNG+++ G
Sbjct: 308 IGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTG 367

Query: 396 VSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKGLV 455
           VSLY+G  +      LVY  K    S +LC+  SLD  +V GKIV+CDRG + RV KG V
Sbjct: 368 VSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAV 427

Query: 456 VKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAFQGT 515
           V+ AG +GMI+AN  ++GE LV D+H+LPA AVG   GD ++ Y  S + PTA + F+GT
Sbjct: 428 VRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGT 487

Query: 516 IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 575
           ++ +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T+FN
Sbjct: 488 VLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFN 547

Query: 576 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTA 635
           I+SGTSM+CPH+SG A LLK+AHP+WSP+A++SA+MTTA + DN   P+ + +    S  
Sbjct: 548 IMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNP 607

Query: 636 YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTP-VTCPAKKPLPE 695
           Y  G+GHV+   A+ PGLVYDI+  +Y+ FLCS+ Y    I  I + P V C  K   P 
Sbjct: 608 YAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPG 667

Query: 696 NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVFST 755
            LNYPS    FS L  G     + R  TNVG  +SVY+  +     V + VKPSKL F +
Sbjct: 668 QLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKS 727

Query: 756 AVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPL 797
             +K+ + V      + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 728 VGEKKRYTVTF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of Tan0015600 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 2.5e-195
Identity = 380/789 (48.16%), Postives = 501/789 (63.50%), Query Frame = 0

Query: 41  PLFFFLL---LLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTS---------- 100
           P  F +L   L+     T +  S  KT++  +D+ + P  +  H  WY+S          
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS 69

Query: 101 --EFAESPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLG 160
             E   + +IL+ Y T FHG +A LTQE+ + + +   V+AV  + R +LHTTRSP FLG
Sbjct: 70  QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLG 129

Query: 161 LRNQRG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAK 220
           L  Q    +W++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +
Sbjct: 130 LERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 189

Query: 221 NCNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQA 280
           NCNRKIVGAR F +G+EA +        I++ +EY+SPRD DGHGTHTA+T AG     A
Sbjct: 190 NCNRKIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGA 249

Query: 281 SLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDG 340
           +L G+A G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG  
Sbjct: 250 NLFGFAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG-- 309

Query: 341 VSSPYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAV 400
             S Y  D ++I  +GA   GVFVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA 
Sbjct: 310 -VSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPAT 369

Query: 401 VTLGNGRKIYGVSLYAGAPL--NGTMYPLVYPGK--SGVLSVSLCMENSLDPKLVSGKIV 460
           V +G  R   GVSLY G  +      YPLVY G+  S     S C++ +LD + V+GKIV
Sbjct: 370 VKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIV 429

Query: 461 ICDRGSSPRVAKGLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYA 520
           ICDRG +PRV KG VVK+AG +GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA
Sbjct: 430 ICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYA 489

Query: 521 SSSANPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAV 580
            +S   TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPDL+APGVNILAAWT  +
Sbjct: 490 MTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDM 549

Query: 581 GPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNR 640
            P+ L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA + DN 
Sbjct: 550 APSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNM 609

Query: 641 RQPMTEESTGKASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVIT 700
            +P+T+ S    S+ YD GAGH++   A DPGLVYDI   +Y  FLC+       ++V T
Sbjct: 610 FKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFT 669

Query: 701 R-TPVTCP---AKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIE 760
           + +  TC    AK   P NLNYP+I A+F   +      +  RT TNVGP  S Y+  + 
Sbjct: 670 KHSNRTCKHTLAKN--PGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVS 729

Query: 761 APKGVTVKVKPSKLVFSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSP 805
             KG +V V+P  L F++  +K S+ V      +          FG L W    H VRSP
Sbjct: 730 PFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSP 780

BLAST of Tan0015600 vs. NCBI nr
Match: XP_038901136.1 (subtilisin-like protease SBT1.6 [Benincasa hispida])

HSP 1 Score: 1478.4 bits (3826), Expect = 0.0e+00
Identity = 746/774 (96.38%), Postives = 757/774 (97.80%), Query Frame = 0

Query: 31  MAACFATSLAPLFFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA 90
           MAA  +TSL+P FFFLLLL  H STVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAFLSTSLSP-FFFLLLLTLH-STVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 91  ESPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 150
           ESPKILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESPKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 151 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 210
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 211 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 270
           GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 271 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 330
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 331 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGR 390
           DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 391 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVA 450
           KI GVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK V+GKIVICDRGSSPRVA
Sbjct: 361 KISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVA 420

Query: 451 KGLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIA 510
           KGLVVKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGDAMKAYASSS NP ATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPIATIA 480

Query: 511 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 570
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 571 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 630
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 631 ASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKP 690
           AST YDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYG KMIQVITRTPV CPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVKCPAKKP 660

Query: 691 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLV 750
           LPENLNYPSIV+VFSSLSKGWSTKSFIRT TNVGP+NS YRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVSVFSSLSKGWSTKSFIRTVTNVGPSNSAYRAKIEAPKGVTVKVKPSKLV 720

Query: 751 FSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           FST VKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 772

BLAST of Tan0015600 vs. NCBI nr
Match: KAG7010855.1 (Subtilisin-like protease SBT1.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1464.1 bits (3789), Expect = 0.0e+00
Identity = 732/770 (95.06%), Postives = 752/770 (97.66%), Query Frame = 0

Query: 36  ATSLAPLFFFLLLLLFHRSTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFAESPK 95
           A  L+PLFFF  LL+ HR TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFAES K
Sbjct: 2   AAYLSPLFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHK 61

Query: 96  ILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 155
           ILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS
Sbjct: 62  ILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 121

Query: 156 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARF 215
           DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGARF
Sbjct: 122 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARF 181

Query: 216 FSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAK 275
           FSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIAK
Sbjct: 182 FSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAK 241

Query: 276 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 335
           GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA
Sbjct: 242 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 301

Query: 336 IGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYG 395
           IGAYGAASRG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR+I G
Sbjct: 302 IGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISG 361

Query: 396 VSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKGLV 455
           VSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPK+V+GKIVICDRGSSPRVAKGLV
Sbjct: 362 VSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLV 421

Query: 456 VKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAFQGT 515
           VKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGD+MKAYASSS NPTATIAFQGT
Sbjct: 422 VKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGT 481

Query: 516 IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 575
           IIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTEFN
Sbjct: 482 IIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 541

Query: 576 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTA 635
           ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGKAST 
Sbjct: 542 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTP 601

Query: 636 YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKPLPEN 695
           YDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYG KMIQVITRTPVTCPAKKPLPEN
Sbjct: 602 YDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPEN 661

Query: 696 LNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVFSTA 755
           LNYPSIVAVFSSLSKGWSTKSFIRT TNVGP NSVYRAKIEAPKGVTVKVKPSKLVFS A
Sbjct: 662 LNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA 721

Query: 756 VKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
            KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 TKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 771

BLAST of Tan0015600 vs. NCBI nr
Match: XP_022985703.1 (subtilisin-like protease SBT1.6 [Cucurbita maxima])

HSP 1 Score: 1460.3 bits (3779), Expect = 0.0e+00
Identity = 733/770 (95.19%), Postives = 753/770 (97.79%), Query Frame = 0

Query: 36  ATSLAPLFFFLLLLLFHRSTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFAESPK 95
           A  L+PLFFF LL++ HR TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFAES K
Sbjct: 2   AAYLSPLFFFFLLIV-HRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHK 61

Query: 96  ILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 155
           ILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS
Sbjct: 62  ILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 121

Query: 156 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARF 215
           DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGARF
Sbjct: 122 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARF 181

Query: 216 FSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAK 275
           FSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIAK
Sbjct: 182 FSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAK 241

Query: 276 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 335
           GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA
Sbjct: 242 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 301

Query: 336 IGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYG 395
           IGAYGAASRG+FVSSS GNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR+I G
Sbjct: 302 IGAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISG 361

Query: 396 VSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKGLV 455
           VSLYAGAPLN TM+PLVYPGKSGVLSVSLCM+NSLDPK+V+GKIVICDRGSSPRVAKGLV
Sbjct: 362 VSLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLV 421

Query: 456 VKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAFQGT 515
           VKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGD+MKAYASSSANPTATIAFQGT
Sbjct: 422 VKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAFQGT 481

Query: 516 IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 575
           IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN
Sbjct: 482 IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 541

Query: 576 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTA 635
           ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGKAST 
Sbjct: 542 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTP 601

Query: 636 YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKPLPEN 695
           YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYG KMIQVITRTPVTCPAKKPLPEN
Sbjct: 602 YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPLPEN 661

Query: 696 LNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVFSTA 755
           LNYPSIVAVFSSLSKGWSTKSFIRT TNVGP NSVYRAKIEAPKGVTVKVKPSKLVFS A
Sbjct: 662 LNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA 721

Query: 756 VKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           +KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 MKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of Tan0015600 vs. NCBI nr
Match: XP_008457681.1 (PREDICTED: subtilisin-like protease SBT1.6 [Cucumis melo])

HSP 1 Score: 1459.9 bits (3778), Expect = 0.0e+00
Identity = 732/774 (94.57%), Postives = 751/774 (97.03%), Query Frame = 0

Query: 31  MAACFATSLAPLFFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA 90
           MAA  +TSL+P FF  LLLL   STVS+ SPLKTFI R+DRFSKPSVFPTHYHWYTSEF 
Sbjct: 1   MAAFLSTSLSPFFFSFLLLL---STVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 91  ESPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 150
           +SP+ILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 151 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 210
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 211 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 270
           GARFFSKGHEAG+NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 271 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 330
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 331 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGR 390
           DPIAIGAYGAAS+GVFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VVTLGNGR
Sbjct: 301 DPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 391 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVA 450
           KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK+V+GKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 451 KGLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIA 510
           KGLVVKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 511 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 570
           FQGTIIGIKPAPVVASFSARGPNGL PEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 571 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 630
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASI DNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEESTGK 600

Query: 631 ASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKP 690
            ST YDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVITRTPV CP KKP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 691 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLV 750
           LPENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP+NSVYR KIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 751 FSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           FST VKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of Tan0015600 vs. NCBI nr
Match: KAG6571024.1 (Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1459.5 bits (3777), Expect = 0.0e+00
Identity = 732/771 (94.94%), Postives = 752/771 (97.54%), Query Frame = 0

Query: 36  ATSLAPL-FFFLLLLLFHRSTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFAESP 95
           A  L+PL FFF  LL+ HR TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFAES 
Sbjct: 2   AAYLSPLFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESH 61

Query: 96  KILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLW 155
           KILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLW
Sbjct: 62  KILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLW 121

Query: 156 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 215
           SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGAR
Sbjct: 122 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGAR 181

Query: 216 FFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 275
           FFSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIA
Sbjct: 182 FFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIA 241

Query: 276 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPI 335
           KGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPI
Sbjct: 242 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPI 301

Query: 336 AIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIY 395
           AIGAYGAASRG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR+I 
Sbjct: 302 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRIS 361

Query: 396 GVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKGL 455
           GVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPK+V+GKIVICDRGSSPRVAKGL
Sbjct: 362 GVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGL 421

Query: 456 VVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAFQG 515
           VVKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGD+MKAYASSS NPTATIAFQG
Sbjct: 422 VVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQG 481

Query: 516 TIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEF 575
           TIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTEF
Sbjct: 482 TIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEF 541

Query: 576 NILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAST 635
           NILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGKAST
Sbjct: 542 NILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKAST 601

Query: 636 AYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKPLPE 695
            YDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYG KMIQVITRTPVTCPAKKPLPE
Sbjct: 602 PYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPE 661

Query: 696 NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVFST 755
           NLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP NSVYRAKIEAPKGVTVKVKPSKLVFS 
Sbjct: 662 NLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSA 721

Query: 756 AVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           A KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 ATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 772

BLAST of Tan0015600 vs. ExPASy TrEMBL
Match: A0A6J1JEF5 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3 SV=1)

HSP 1 Score: 1460.3 bits (3779), Expect = 0.0e+00
Identity = 733/770 (95.19%), Postives = 753/770 (97.79%), Query Frame = 0

Query: 36  ATSLAPLFFFLLLLLFHRSTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFAESPK 95
           A  L+PLFFF LL++ HR TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFAES K
Sbjct: 2   AAYLSPLFFFFLLIV-HRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHK 61

Query: 96  ILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 155
           ILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS
Sbjct: 62  ILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 121

Query: 156 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARF 215
           DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGARF
Sbjct: 122 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARF 181

Query: 216 FSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAK 275
           FSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIAK
Sbjct: 182 FSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAK 241

Query: 276 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 335
           GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA
Sbjct: 242 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 301

Query: 336 IGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYG 395
           IGAYGAASRG+FVSSS GNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR+I G
Sbjct: 302 IGAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISG 361

Query: 396 VSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKGLV 455
           VSLYAGAPLN TM+PLVYPGKSGVLSVSLCM+NSLDPK+V+GKIVICDRGSSPRVAKGLV
Sbjct: 362 VSLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLV 421

Query: 456 VKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAFQGT 515
           VKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGD+MKAYASSSANPTATIAFQGT
Sbjct: 422 VKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAFQGT 481

Query: 516 IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 575
           IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN
Sbjct: 482 IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 541

Query: 576 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTA 635
           ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGKAST 
Sbjct: 542 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTP 601

Query: 636 YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKPLPEN 695
           YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYG KMIQVITRTPVTCPAKKPLPEN
Sbjct: 602 YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPLPEN 661

Query: 696 LNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVFSTA 755
           LNYPSIVAVFSSLSKGWSTKSFIRT TNVGP NSVYRAKIEAPKGVTVKVKPSKLVFS A
Sbjct: 662 LNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAA 721

Query: 756 VKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           +KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 MKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of Tan0015600 vs. ExPASy TrEMBL
Match: A0A1S3C7D0 (subtilisin-like protease SBT1.6 OS=Cucumis melo OX=3656 GN=LOC103497323 PE=3 SV=1)

HSP 1 Score: 1459.9 bits (3778), Expect = 0.0e+00
Identity = 732/774 (94.57%), Postives = 751/774 (97.03%), Query Frame = 0

Query: 31  MAACFATSLAPLFFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA 90
           MAA  +TSL+P FF  LLLL   STVS+ SPLKTFI R+DRFSKPSVFPTHYHWYTSEF 
Sbjct: 1   MAAFLSTSLSPFFFSFLLLL---STVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 91  ESPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 150
           +SP+ILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 151 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 210
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 211 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 270
           GARFFSKGHEAG+NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 271 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 330
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 331 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGR 390
           DPIAIGAYGAAS+GVFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VVTLGNGR
Sbjct: 301 DPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 391 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVA 450
           KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK+V+GKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 451 KGLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIA 510
           KGLVVKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 511 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 570
           FQGTIIGIKPAPVVASFSARGPNGL PEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 571 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 630
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASI DNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEESTGK 600

Query: 631 ASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKP 690
            ST YDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVITRTPV CP KKP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 691 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLV 750
           LPENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP+NSVYR KIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 751 FSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           FST VKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of Tan0015600 vs. ExPASy TrEMBL
Match: A0A0A0LJ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1)

HSP 1 Score: 1458.0 bits (3773), Expect = 0.0e+00
Identity = 730/774 (94.32%), Postives = 751/774 (97.03%), Query Frame = 0

Query: 31  MAACFATSLAPLFFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA 90
           MAA  +TSL+  FF  LLLL   STVS+ SPLKTFI R+DRFSKPSVFPTHYHWYTSEF 
Sbjct: 1   MAAFLSTSLSSFFFSFLLLL---STVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 91  ESPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 150
           +SP+ILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 151 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 210
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 211 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 270
           GARFFSKGHEAG+NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 271 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 330
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 331 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGR 390
           DPIAIG+YGAAS+GVFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VVTLGNGR
Sbjct: 301 DPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 391 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVA 450
           KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK+V+GKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 451 KGLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIA 510
           KGLVVKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 511 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 570
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFD RK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRK 540

Query: 571 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 630
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 631 ASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKP 690
            ST YDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVITRTPV CP KKP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 691 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLV 750
           LPENLNYPSIV VFSSLSKGWSTKSFIRTATNVGP+NSVYR KIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 751 FSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           FST VKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of Tan0015600 vs. ExPASy TrEMBL
Match: A0A6J1FYE5 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE=3 SV=1)

HSP 1 Score: 1458.0 bits (3773), Expect = 0.0e+00
Identity = 730/773 (94.44%), Postives = 751/773 (97.15%), Query Frame = 0

Query: 33  ACFATSLAPLFFFLLLLLFHRSTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFAE 92
           A + + L   FFF  LL+ HR TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFAE
Sbjct: 2   AAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAE 61

Query: 93  SPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 152
           S KILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG
Sbjct: 62  SHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 121

Query: 153 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVG 212
           LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVG
Sbjct: 122 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVG 181

Query: 213 ARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASG 272
           ARFFSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASG
Sbjct: 182 ARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASG 241

Query: 273 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD 332
           IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD
Sbjct: 242 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD 301

Query: 333 PIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRK 392
           PIAIGAYGAASRG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVVTLGNGR+
Sbjct: 302 PIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRR 361

Query: 393 IYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAK 452
           I GVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPK+V+GKIVICDRGSSPRVAK
Sbjct: 362 ISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAK 421

Query: 453 GLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAF 512
           GLVVKKAG VGMILANGISNGEGLVGDAH+LPACAVGSDEGD+MKAYASSS NPTATIAF
Sbjct: 422 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAF 481

Query: 513 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 572
           QGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKT
Sbjct: 482 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKT 541

Query: 573 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKA 632
           EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGKA
Sbjct: 542 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKA 601

Query: 633 STAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCPAKKPL 692
           ST YDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYG KMIQVITRTPVTCPAKKPL
Sbjct: 602 STPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPL 661

Query: 693 PENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVF 752
           PENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP NSVYRAKIEAPKGVTVKVKPSKLVF
Sbjct: 662 PENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVF 721

Query: 753 STAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           S A KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 SAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of Tan0015600 vs. ExPASy TrEMBL
Match: A0A5D3BLE2 (Subtilisin-like protease SBT1.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001210 PE=3 SV=1)

HSP 1 Score: 1433.3 bits (3709), Expect = 0.0e+00
Identity = 711/741 (95.95%), Postives = 727/741 (98.11%), Query Frame = 0

Query: 64  TFIFRVDRFSKPSVFPTHYHWYTSEFAESPKILHVYDTVFHGFSATLTQEQVDSIGKHPS 123
           TFI R+DRFSKPSVFPTHYHWYTSEF +SP+ILHVYDTVFHGFSATLTQEQVDSIGKHPS
Sbjct: 2   TFIVRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQEQVDSIGKHPS 61

Query: 124 VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 183
           VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL
Sbjct: 62  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 121

Query: 184 GPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRD 243
           GPIPRRWKGVC+TGTKFTAKNCNRKIVGARFFSKGHEAG+NAAGPIIGINDTIEYRSPRD
Sbjct: 122 GPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 181

Query: 244 ADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 303
           ADGHGTHTASTAAGRH+FQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF
Sbjct: 182 ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 241

Query: 304 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTN 363
           DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS+GVFVSSSAGNDGPNGMSVTN
Sbjct: 242 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN 301

Query: 364 LAPWITTVGAGTIDRNFPAVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 423
           LAPW+TTVGAGTIDRNFP+VVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL
Sbjct: 302 LAPWVTTVGAGTIDRNFPSVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 361

Query: 424 CMENSLDPKLVSGKIVICDRGSSPRVAKGLVVKKAGAVGMILANGISNGEGLVGDAHVLP 483
           CMENSLDPK+V+GKIVICDRGSSPRVAKGLVVKKAG VGMILANGISNGEGLVGDAH+LP
Sbjct: 362 CMENSLDPKVVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 421

Query: 484 ACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPD 543
           ACAVGSDEGDAMKAYASSS NPTATIAFQGTIIGIKPAPVVASFSARGPNGL PEILKPD
Sbjct: 422 ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLTPEILKPD 481

Query: 544 LIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 603
           +IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA
Sbjct: 482 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 541

Query: 604 ALRSAMMTTASITDNRRQPMTEESTGKASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVN 663
           ALRSAMMTTASI DNRRQPMTEESTGK ST YDFGAGHVNLGLAMDPGL+YDITNTDY+N
Sbjct: 542 ALRSAMMTTASIIDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYIN 601

Query: 664 FLCSIGYGAKMIQVITRTPVTCPAKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNV 723
           FLCSIGYG KMIQVITRTPV CP KKPLPENLNYPSIVAVFSSLSKGWSTKSFIRT TNV
Sbjct: 602 FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNV 661

Query: 724 GPTNSVYRAKIEAPKGVTVKVKPSKLVFSTAVKKQSFVVAISADNQNLALGDVGAVFGWL 783
           GP+NSVYR KIEAPKGVTVKVKPSKLVFST VKKQSFVVAISADNQNLALGDVGAVFGWL
Sbjct: 662 GPSNSVYRVKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL 721

Query: 784 SWSDGKHVVRSPLVVTQLEPL 805
           SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 SWSDGKHVVRSPLVVTQLEPL 742

BLAST of Tan0015600 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 560/761 (73.59%), Postives = 659/761 (86.60%), Query Frame = 0

Query: 46  LLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAESPKILHVYDTVFHG 105
           LL L F   + +A    KTFIFR+D  S PS+FPTHYHWY++EFAE  +I+HVY TVFHG
Sbjct: 9   LLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTVFHG 68

Query: 106 FSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIG 165
           FSA +T ++ D++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGSDVIIG
Sbjct: 69  FSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIG 128

Query: 166 VFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGSNA 225
           VFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG +     
Sbjct: 129 VFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQ----- 188

Query: 226 AGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVY 285
           A  I GIN T+E+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPKAR+A Y
Sbjct: 189 AAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAY 248

Query: 286 KVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGV 345
           KVCWK+SGC DSDILAAFDAAV DGVDVISISIGGGDG++SPYYLDPIAIG+YGAAS+G+
Sbjct: 249 KVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGI 308

Query: 346 FVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYGVSLYAGAPLNG 405
           FVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA   LG+G ++ GVSLYAG PLNG
Sbjct: 309 FVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNG 368

Query: 406 TMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKGLVVKKAGAVGMIL 465
            M+P+VYPGKSG+ S SLCMEN+LDPK V GKIVICDRGSSPRVAKGLVVKKAG VGMIL
Sbjct: 369 RMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMIL 428

Query: 466 ANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAFQGTIIGIKPAPVVA 525
           ANG SNGEGLVGDAH++PACAVGS+EGD +KAYASS  NP A+I F+GTI+GIKPAPV+A
Sbjct: 429 ANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIA 488

Query: 526 SFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPH 585
           SFS RGPNGL+PEILKPDLIAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTSMACPH
Sbjct: 489 SFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPH 548

Query: 586 VSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTAYDFGAGHVNLG 645
           VSGAAALLKSAHPDWSPA +RSAMMTT ++ DN  + + +ESTGK++T YD+G+GH+NLG
Sbjct: 549 VSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLG 608

Query: 646 LAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCP-AKKPLPENLNYPSIVAVF 705
            AM+PGLVYDITN DY+ FLCSIGYG K IQVITRTPV CP  +KP P NLNYPSI AVF
Sbjct: 609 RAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVF 668

Query: 706 SSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVFSTAVKKQSFVVAI 765
            +  +G  +K+ IRTATNVG   +VYRA+IE+P+GVTV VKP +LVF++AVK++S+ V +
Sbjct: 669 PTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTV 728

Query: 766 SADNQNLALGDVGAVFGWLSWSD-GKHVVRSPLVVTQLEPL 805
           + + +N+ LG+ GAVFG ++W D GKHVVRSP+VVTQ++ L
Sbjct: 729 TVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of Tan0015600 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 854.0 bits (2205), Expect = 9.9e-248
Identity = 450/780 (57.69%), Postives = 548/780 (70.26%), Query Frame = 0

Query: 43  FFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA----ESPKILHV 102
           +FF LL L   S+ ++ S   T+I  VD  +KPS+FPTH+HWYTS  A      P I+H 
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHT 65

Query: 103 YDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDS 162
           YDTVFHGFSA LT +    +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +S
Sbjct: 66  YDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEES 125

Query: 163 DYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFS 222
           D+GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF 
Sbjct: 126 DFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFC 185

Query: 223 KGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGV 282
            G+EA +        +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+
Sbjct: 186 GGYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 245

Query: 283 APKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIG 342
           APKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD IAIG
Sbjct: 246 APKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIG 305

Query: 343 AYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYGVS 402
           A+GA  RG+FVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ I GVS
Sbjct: 306 AFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVS 365

Query: 403 LYAGAPLN-GTMYPLVYPGK---SGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKG 462
           +Y G  L+ G MYPLVY G        S SLC+E SLDP LV GKIV+CDRG + R  KG
Sbjct: 366 VYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKG 425

Query: 463 LVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYAS------SSANPT 522
            +V+K G +GMI+ANG+ +GEGLV D HVLPA +VG+  GD ++ Y S      SS +PT
Sbjct: 426 EIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT 485

Query: 523 ATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDF 582
           ATI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  
Sbjct: 486 ATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTS 545

Query: 583 DTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEE 642
           D R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA   DN  +PM +E
Sbjct: 546 DNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDE 605

Query: 643 STGKASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTCP 702
           STG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  ITR    C 
Sbjct: 606 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 665

Query: 703 AKKPLPE--NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKV 762
             +      NLNYPS   VF    +   +  FIRT TNVG ++SVY  KI  P+G TV V
Sbjct: 666 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 725

Query: 763 KPSKLVFSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 805
           +P KL F    +K SFVV +      L+ G      G + WSDGK  V SPLVVT  +PL
Sbjct: 726 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of Tan0015600 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 728.4 bits (1879), Expect = 6.3e-210
Identity = 395/776 (50.90%), Postives = 525/776 (67.65%), Query Frame = 0

Query: 31  MAACFATSLAPLFFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTS--- 90
           M++ F +S A   FFLLL L      S+ S   T+I  + +   PS F  H +WY S   
Sbjct: 1   MSSSFLSSTA---FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLR 60

Query: 91  EFAESPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL- 150
             ++S ++L+ Y+   HGFS  LTQE+ DS+   P V++V  + R +LHTTR+P FLGL 
Sbjct: 61  SISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 120

Query: 151 RNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCN 210
            +   L+ ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CN
Sbjct: 121 EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 180

Query: 211 RKIVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLE 270
           RK++GARFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL 
Sbjct: 181 RKLIGARFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL 240

Query: 271 GYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSS 330
           GYASG A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S
Sbjct: 241 GYASGTARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MS 300

Query: 331 PYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTL 390
            YY D +AIGA+ A  RG+ VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+  L
Sbjct: 301 DYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAIL 360

Query: 391 GNGRKIYGVSLYAGAPLNGTMYPLVYPGK-SGVLSVSLCMENSLDPKLVSGKIVICDRGS 450
           GNG+   GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG 
Sbjct: 361 GNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGI 420

Query: 451 SPRVAKGLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANP 510
           + RV KG VVK AG VGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NP
Sbjct: 421 NARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 480

Query: 511 TATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLD 570
           TA+I+  GT++G+KP+PVVA+FS+RGPN + P ILKPDLIAPGVNILAAWT A GPTGL 
Sbjct: 481 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLA 540

Query: 571 FDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTE 630
            D+R+ EFNI+SGTSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T    +P+ +
Sbjct: 541 SDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLD 600

Query: 631 ESTGKASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTPVTC 690
            +TGK ST +D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y +  I+ ++R   TC
Sbjct: 601 IATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTC 660

Query: 691 -PAKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEA-PKGVTVK 750
            P+K     +LNYPS    F+    G     + RT T+VG   + Y  K+ +   GV + 
Sbjct: 661 DPSKSYSVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKIS 720

Query: 751 VKPSKLVFSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVT 800
           V+P+ L F  A +K+S+ V  + D+   +  +    FG + WSDGKHVV SP+ ++
Sbjct: 721 VEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of Tan0015600 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 711.4 bits (1835), Expect = 7.9e-205
Identity = 377/763 (49.41%), Postives = 505/763 (66.19%), Query Frame = 0

Query: 36  ATSLAPLFFFLLLLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAESPKI 95
           ++S+  +  FL LLL         +  KT+I RV+   KP  F TH+ WYTS+      +
Sbjct: 8   SSSITIITTFLFLLLH-------TTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSESSL 67

Query: 96  LHVYDTVFHGFSATLTQEQVDS-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWS 155
           L+ Y T FHGFSA L   + DS +    S+L +FED    LHTTR+P+FLGL ++ G+  
Sbjct: 68  LYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHD 127

Query: 156 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARF 215
                + VIIGV DTG+ PE RSF D ++  IP +WKG C++G+ F +K CN+K++GAR 
Sbjct: 128 LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARS 187

Query: 216 FSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAK 275
           FSKG +  S       G +   E  SPRD DGHGTHT++TAAG     AS  GYA+G A+
Sbjct: 188 FSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTAR 247

Query: 276 GVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIA 335
           G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D IA
Sbjct: 248 GMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRDTIA 307

Query: 336 IGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVTLGNGRKIYG 395
           IGA+ A  RGVFVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   LGNG+++ G
Sbjct: 308 IGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTG 367

Query: 396 VSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKLVSGKIVICDRGSSPRVAKGLV 455
           VSLY+G  +      LVY  K    S +LC+  SLD  +V GKIV+CDRG + RV KG V
Sbjct: 368 VSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAV 427

Query: 456 VKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYASSSANPTATIAFQGT 515
           V+ AG +GMI+AN  ++GE LV D+H+LPA AVG   GD ++ Y  S + PTA + F+GT
Sbjct: 428 VRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGT 487

Query: 516 IIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFN 575
           ++ +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T+FN
Sbjct: 488 VLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFN 547

Query: 576 ILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTA 635
           I+SGTSM+CPH+SG A LLK+AHP+WSP+A++SA+MTTA + DN   P+ + +    S  
Sbjct: 548 IMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNP 607

Query: 636 YDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVITRTP-VTCPAKKPLPE 695
           Y  G+GHV+   A+ PGLVYDI+  +Y+ FLCS+ Y    I  I + P V C  K   P 
Sbjct: 608 YAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPG 667

Query: 696 NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIEAPKGVTVKVKPSKLVFST 755
            LNYPS    FS L  G     + R  TNVG  +SVY+  +     V + VKPSKL F +
Sbjct: 668 QLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKS 727

Query: 756 AVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPL 797
             +K+ + V      + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 728 VGEKKRYTVTF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of Tan0015600 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 683.7 bits (1763), Expect = 1.8e-196
Identity = 380/789 (48.16%), Postives = 501/789 (63.50%), Query Frame = 0

Query: 41  PLFFFLL---LLLFHRSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTS---------- 100
           P  F +L   L+     T +  S  KT++  +D+ + P  +  H  WY+S          
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS 69

Query: 101 --EFAESPKILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLG 160
             E   + +IL+ Y T FHG +A LTQE+ + + +   V+AV  + R +LHTTRSP FLG
Sbjct: 70  QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLG 129

Query: 161 LRNQRG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAK 220
           L  Q    +W++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +
Sbjct: 130 LERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 189

Query: 221 NCNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQA 280
           NCNRKIVGAR F +G+EA +        I++ +EY+SPRD DGHGTHTA+T AG     A
Sbjct: 190 NCNRKIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGA 249

Query: 281 SLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDG 340
           +L G+A G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG  
Sbjct: 250 NLFGFAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG-- 309

Query: 341 VSSPYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAV 400
             S Y  D ++I  +GA   GVFVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA 
Sbjct: 310 -VSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPAT 369

Query: 401 VTLGNGRKIYGVSLYAGAPL--NGTMYPLVYPGK--SGVLSVSLCMENSLDPKLVSGKIV 460
           V +G  R   GVSLY G  +      YPLVY G+  S     S C++ +LD + V+GKIV
Sbjct: 370 VKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIV 429

Query: 461 ICDRGSSPRVAKGLVVKKAGAVGMILANGISNGEGLVGDAHVLPACAVGSDEGDAMKAYA 520
           ICDRG +PRV KG VVK+AG +GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA
Sbjct: 430 ICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYA 489

Query: 521 SSSANPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAV 580
            +S   TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPDL+APGVNILAAWT  +
Sbjct: 490 MTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDM 549

Query: 581 GPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNR 640
            P+ L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA + DN 
Sbjct: 550 APSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNM 609

Query: 641 RQPMTEESTGKASTAYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVIT 700
            +P+T+ S    S+ YD GAGH++   A DPGLVYDI   +Y  FLC+       ++V T
Sbjct: 610 FKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFT 669

Query: 701 R-TPVTCP---AKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPTNSVYRAKIE 760
           + +  TC    AK   P NLNYP+I A+F   +      +  RT TNVGP  S Y+  + 
Sbjct: 670 KHSNRTCKHTLAKN--PGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVS 729

Query: 761 APKGVTVKVKPSKLVFSTAVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSP 805
             KG +V V+P  L F++  +K S+ V      +          FG L W    H VRSP
Sbjct: 730 PFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSP 780