MC05g_new0176 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g_new0176 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.3e-52
Identity = 120/293 (40.96%), Postives = 157/293 (53.58%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDE-----NEQEAKYTLTSPSRLLLRHN------------------- 60
           M +L+HSGFFA    E       +E  Y +T  S+LLL+ N                   
Sbjct: 88  MRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLDPVMTKP 147

Query: 61  -----TWLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTREC 120
                 W  + D     PF+ AN  + WDY + EP     FN AM  D++L+  +V  +C
Sbjct: 148 WDFLSNWFQNGD---PTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSVVIEKC 207

Query: 121 RWVFEGLKSLVDVGGSRGFMAKAIVEAFPHITCT-------------------------- 180
           + VFEG++SLVDVGG  G +A +I  AFPHI CT                          
Sbjct: 208 KGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGNNLNFVGGDMFV 267

Query: 181 -----------WFLRDWNDEESIQILKKCRYAI-PNRAEGGKVIIIEMVMKNQ-IEDKES 226
                      W L DWNDEES++ILKKC+ AI  +  +GGKVIII+M ++N+  ED ES
Sbjct: 268 DVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVENEKDEDDES 327

BLAST of MC05g_new0176 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 206.1 bits (523), Expect = 4.3e-52
Identity = 117/287 (40.77%), Postives = 152/287 (52.96%), Query Frame = 0

Query: 1   MHLLVHSGFF-AQTHDENEQEAKYTLTSPSRLLLRHNT---------------------- 60
           M +LVHSGF  AQ   + ++E  Y LT  SRLLL  ++                      
Sbjct: 74  MRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMDDSLSIRPLVLAMLDPILTKPWHYL 133

Query: 61  --WLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVF 120
             W  + D     PF  A ++S WDY   EP   N+FN+AM  D++L+  ++ +E + VF
Sbjct: 134 SAWFQNDD---PTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVLLKEGQGVF 193

Query: 121 EGLKSLVDVGGSRGFMAKAIVEAFPHITCT------------------------------ 180
            GL SLVDVGG  G +AKAI  AFPH+ CT                              
Sbjct: 194 AGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSKNLNYFAGDMFEAIPP 253

Query: 181 -------WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIETQLC 226
                  W L DW++EE ++ILK+CR AIP++  GGKVIII+M+M     D +S ETQL 
Sbjct: 254 ADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYKSTETQLF 313

BLAST of MC05g_new0176 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.1e-47
Identity = 111/288 (38.54%), Postives = 154/288 (53.47%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENE-QEAKYTLTSPSRLLLRH------------------------ 60
           + +LVHSGFFA T  ++E  E  Y  T  S+LLL++                        
Sbjct: 87  LRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLKNHPMSMSPCLLGLVNPTMVAPMHFF 146

Query: 61  NTWLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVF 120
           + W   SD+    PFE  +  SLW Y  + P     FN+AM  +++L   +V +EC+   
Sbjct: 147 SDWFKRSDD--MTPFEATHGASLWKYFGETPHMAEIFNEAMGCETRLAMSVVLKECKGKL 206

Query: 121 EGLKSLVDVGGSRGFMAKAIVEAFPHITCT------------------------------ 180
           EG+ SLVDVGG  G + +AI EAFP++ CT                              
Sbjct: 207 EGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGSNNLEFVSGDMFQFIPP 266

Query: 181 -------WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDK-ESIETQL 226
                  W L DWNDEE I+ILK+C+ AIP++ EGGK+III+MV+ +  +   ES ETQL
Sbjct: 267 ADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVVNDHNKGSYESTETQL 326

BLAST of MC05g_new0176 vs. ExPASy Swiss-Prot
Match: Q84KK4 (Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 8.0e-43
Identity = 104/292 (35.62%), Postives = 149/292 (51.03%), Query Frame = 0

Query: 1   MHLLVHSGFFAQT----HDENEQEAKYTLTSPSRLLLRHNT------------------- 60
           + LL H+GFFA+T     +  E+E  Y LT PS+LL++ N+                   
Sbjct: 76  LRLLTHNGFFAKTTVSRGEGAEEETAYGLTPPSKLLVKSNSTCLAPIVKGALHPSSLDMW 135

Query: 61  -----WLCSSDERYSCPFEMANKKSLWDYVAQ--EPDFRNTFNQAMMFDSQLIGRIVTRE 120
                W    +E  +  FE A  +S W+++ +  E D  + F +AM  DS +  ++  +E
Sbjct: 136 RSSKKWFLEDNEELTL-FESATGESFWEFLNKETESDTLSMFQEAMAADSHMF-KLALKE 195

Query: 121 CRWVFEGLKSLVDVGGSRGFMAKAIVEAFPHITCT------------------------- 180
           C+ VFEGL SLVDV G RG + K I EAFPH+ CT                         
Sbjct: 196 CKHVFEGLGSLVDVAGGRGGVTKLIREAFPHVKCTVFDQPQVVANLTGDENLNFVGGDMF 255

Query: 181 ------------WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESI 226
                       W L DWNDE S++ILK C+ AI  R + GKVIII++ +    +D+E  
Sbjct: 256 KSVPPADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKEGKVIIIDISIDETSDDRELT 315

BLAST of MC05g_new0176 vs. ExPASy Swiss-Prot
Match: B0EXJ8 (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.3e-42
Identity = 106/286 (37.06%), Postives = 145/286 (50.70%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRH------------------NTWLCSS 60
           M +L  +GF ++   + E E  Y+LT  SR+LL+                     W   S
Sbjct: 72  MRVLAKNGFCSEEQLDGETEPLYSLTPSSRILLKKEPLNLRGIVLTMADPVQLKAWESLS 131

Query: 61  D-----ERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVFEG 120
           D     +  S  FE A+ K+ W Y ++  +    FN+AM  DSQLI +++  E +++FEG
Sbjct: 132 DWYQNEDDSSTAFETAHGKNFWGYSSEHMEHAEFFNEAMASDSQLISKLLIGEYKFLFEG 191

Query: 121 LKSLVDVGGSRGFMAKAIVEAFPHITCT-------------------------------- 180
           L SLVD+GG  G +AKAI + FP + CT                                
Sbjct: 192 LASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKENVEFVAGDMFEKIPSAN 251

Query: 181 -----WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIETQLCDD 226
                W L DWNDE+ ++ILK C+ AIP  A+GGKVIII+MVM +  +D   ++TQ   D
Sbjct: 252 AIFLKWILHDWNDEDCVKILKSCKKAIP--AKGGKVIIIDMVMYSDKKDDHLVKTQTSMD 311

BLAST of MC05g_new0176 vs. NCBI nr
Match: XP_022152376.1 (trans-resveratrol di-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 353 bits (906), Expect = 3.18e-119
Identity = 184/289 (63.67%), Postives = 201/289 (69.55%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRHNT----------------------- 60
           M LLVHSGFF QTHDENEQE KYTLTS SRLLLRHNT                       
Sbjct: 69  MSLLVHSGFFVQTHDENEQEVKYTLTSTSRLLLRHNTPFEHISLLLLIFNKAMMASYETM 128

Query: 61  --WLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVF 120
             W  S+DER+SC FE  N KS+WDYVAQEPDF N  N+AMM+DSQLIGR+VTRECR VF
Sbjct: 129 SSWFRSTDERHSCAFETTNDKSVWDYVAQEPDFSNILNKAMMYDSQLIGRVVTRECRRVF 188

Query: 121 EGLKSLVDVGGSRGFMAKAIVEAFPHITCT------------------------------ 180
           +GLKSLVDVGG+RGFMAKAI EAFPHITC                               
Sbjct: 189 KGLKSLVDVGGNRGFMAKAIAEAFPHITCIVFDLPHVVDNLQQTTENLNFIAGNMFEAKI 248

Query: 181 ---------WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIETQ 225
                    W L DWNDE+SI+ILK C+ AIP+RAEGGKVIIIEMV +NQIEDKESIETQ
Sbjct: 249 PPANAVLLKWVLHDWNDEKSIKILKNCKDAIPSRAEGGKVIIIEMVTENQIEDKESIETQ 308

BLAST of MC05g_new0176 vs. NCBI nr
Match: XP_022158734.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 310 bits (795), Expect = 2.16e-102
Identity = 166/289 (57.44%), Postives = 188/289 (65.05%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRHNT----------------------- 60
           M LLVH+GFF QTH +NEQE +Y LT  SRLLL  NT                       
Sbjct: 69  MRLLVHAGFFIQTHGDNEQE-EYALTPSSRLLLCRNTAFENIPFVFLILNRAMMAPLETM 128

Query: 61  --WLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVF 120
             WLCS DE  +  FEMAN KS+WDYV+QE DF   F+Q M+ DSQLIG++VTRECR VF
Sbjct: 129 SSWLCSVDEHSNSAFEMANGKSIWDYVSQEHDFGKLFHQTMVHDSQLIGKVVTRECREVF 188

Query: 121 EGLKSLVDVGGSRGFMAKAIVEAFPHITCT------------------------------ 180
           EGLKSLVDVGGS GFMAKAI EAFPHITCT                              
Sbjct: 189 EGLKSLVDVGGSTGFMAKAIAEAFPHITCTVFDLPQVAANSQQTTKNLSFIGGDMFEAKI 248

Query: 181 ---------WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIETQ 225
                    W L DWNDEESI+ILK C+ AIP+RAE GKVI+I+MV++NQIEDKES+E Q
Sbjct: 249 PPANAVLLKWILHDWNDEESIRILKNCKDAIPSRAEXGKVIVIDMVVENQIEDKESVEAQ 308

BLAST of MC05g_new0176 vs. NCBI nr
Match: XP_022152414.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 300 bits (767), Expect = 4.04e-98
Identity = 164/290 (56.55%), Postives = 186/290 (64.14%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDEN-EQEAKYTLTSPSRLLLRHNT---------------------- 60
           M LLVHSGFFA+TH EN EQE KY +T  SRLLLRHN                       
Sbjct: 69  MRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHHAMMASWET 128

Query: 61  ---WLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWV 120
              WLCS DE YS  FEMAN KS+W+Y+A+EP+F N F++ + +DSQLIGRIVT ECR V
Sbjct: 129 MSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTECREV 188

Query: 121 FEGLKSLVDVGGSRGFMAKAIVEAFPHITCTWF--------------------------- 180
           FEGLKS+VDVGG  G MAKAIVEAFPHIT   F                           
Sbjct: 189 FEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDMFEAK 248

Query: 181 ------------LRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIET 225
                       L +WNDEESIQILK C+ AI + AEGGKVIIIE+ ++ Q+EDKESIET
Sbjct: 249 IPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKESIET 308

BLAST of MC05g_new0176 vs. NCBI nr
Match: XP_038902252.1 (probable O-methyltransferase 3 [Benincasa hispida])

HSP 1 Score: 273 bits (698), Expect = 1.54e-87
Identity = 154/299 (51.51%), Postives = 178/299 (59.53%), Query Frame = 0

Query: 1   MHLLVHSGFFA-QTHDENEQEA---KYTLTSPSRLLLRHN-------------------- 60
           M LLVHSGFF  QTHD   +EA   KY+LT  SRLLLRH+                    
Sbjct: 70  MRLLVHSGFFTTQTHDHENEEAEDLKYSLTPSSRLLLRHSRSTTFQAIPFLFLCLDKASI 129

Query: 61  -------TWLCSSD---ERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRI 120
                  +W CSS+   + YS  FEMAN K +W+Y AQEP F N F   M+ DS++IG+I
Sbjct: 130 ASWQCLSSWFCSSNNNGQHYSNAFEMANGKFIWNYAAQEPSFANLFQHTMVCDSEIIGKI 189

Query: 121 VTRECRWVFEGLKSLVDVGGSRGFMAKAIVEAFPHITCT--------------------- 180
           V  EC  VFEGL SLVDVGG  G M KAIVEAFPHITCT                     
Sbjct: 190 VKEECGEVFEGLNSLVDVGGGVGVMGKAIVEAFPHITCTVFDLPHVISNQLLQTQTNLRF 249

Query: 181 -------------------WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQ 225
                              W L +WNDE+SI+ILKKC+ AIP+R  GGK++II+MVM+N+
Sbjct: 250 VGGDMFEENIPPANAVLLKWVLHNWNDEQSIKILKKCKDAIPSRERGGKLVIIDMVMENK 309

BLAST of MC05g_new0176 vs. NCBI nr
Match: XP_022935024.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 272 bits (695), Expect = 2.84e-87
Identity = 153/292 (52.40%), Postives = 181/292 (61.99%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRHN------------------------ 60
           M LLVHSGFFA+TH        ++LT+PSRLLLR N                        
Sbjct: 69  MRLLVHSGFFAETHS-----GFFSLTTPSRLLLRQNHNAFDTTPLLLLLTSPVFMAPWET 128

Query: 61  --TWLCSSDERY-SCPFEMANKKSLWDYVAQEPD-FRNTFNQAMMFDSQLIGRIVTRECR 120
              WLCS D+ + S  FEMAN  ++WDY+AQ+P  F N F+Q ++ DSQLIG++VT ECR
Sbjct: 129 MSNWLCSDDKDHNSTAFEMANGNTIWDYIAQQPSGFGNLFHQTLIHDSQLIGKVVTSECR 188

Query: 121 WVFEGLKSLVDVGGSRGFMAKAIVEAFPHITCTWF------------------------- 180
            +FEGL SLVDVGG  G MAKAIVEAFPHI+C  F                         
Sbjct: 189 GMFEGLTSLVDVGGGAGAMAKAIVEAFPHISCIVFDLPQVVASQEPQQAKKNLNFIGGDM 248

Query: 181 ---------------LRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKES 224
                          L DW+DEESIQILK C+ AIP+RAEGGKVIIIEMV + Q  DKES
Sbjct: 249 FEKIPQANALLLKLVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEMVFEEQ-RDKES 308

BLAST of MC05g_new0176 vs. ExPASy TrEMBL
Match: A0A6J1DHJ0 (trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111020124 PE=3 SV=1)

HSP 1 Score: 353 bits (906), Expect = 1.54e-119
Identity = 184/289 (63.67%), Postives = 201/289 (69.55%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRHNT----------------------- 60
           M LLVHSGFF QTHDENEQE KYTLTS SRLLLRHNT                       
Sbjct: 69  MSLLVHSGFFVQTHDENEQEVKYTLTSTSRLLLRHNTPFEHISLLLLIFNKAMMASYETM 128

Query: 61  --WLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVF 120
             W  S+DER+SC FE  N KS+WDYVAQEPDF N  N+AMM+DSQLIGR+VTRECR VF
Sbjct: 129 SSWFRSTDERHSCAFETTNDKSVWDYVAQEPDFSNILNKAMMYDSQLIGRVVTRECRRVF 188

Query: 121 EGLKSLVDVGGSRGFMAKAIVEAFPHITCT------------------------------ 180
           +GLKSLVDVGG+RGFMAKAI EAFPHITC                               
Sbjct: 189 KGLKSLVDVGGNRGFMAKAIAEAFPHITCIVFDLPHVVDNLQQTTENLNFIAGNMFEAKI 248

Query: 181 ---------WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIETQ 225
                    W L DWNDE+SI+ILK C+ AIP+RAEGGKVIIIEMV +NQIEDKESIETQ
Sbjct: 249 PPANAVLLKWVLHDWNDEKSIKILKNCKDAIPSRAEGGKVIIIEMVTENQIEDKESIETQ 308

BLAST of MC05g_new0176 vs. ExPASy TrEMBL
Match: A0A6J1E096 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE=3 SV=1)

HSP 1 Score: 310 bits (795), Expect = 1.05e-102
Identity = 166/289 (57.44%), Postives = 188/289 (65.05%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRHNT----------------------- 60
           M LLVH+GFF QTH +NEQE +Y LT  SRLLL  NT                       
Sbjct: 69  MRLLVHAGFFIQTHGDNEQE-EYALTPSSRLLLCRNTAFENIPFVFLILNRAMMAPLETM 128

Query: 61  --WLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVF 120
             WLCS DE  +  FEMAN KS+WDYV+QE DF   F+Q M+ DSQLIG++VTRECR VF
Sbjct: 129 SSWLCSVDEHSNSAFEMANGKSIWDYVSQEHDFGKLFHQTMVHDSQLIGKVVTRECREVF 188

Query: 121 EGLKSLVDVGGSRGFMAKAIVEAFPHITCT------------------------------ 180
           EGLKSLVDVGGS GFMAKAI EAFPHITCT                              
Sbjct: 189 EGLKSLVDVGGSTGFMAKAIAEAFPHITCTVFDLPQVAANSQQTTKNLSFIGGDMFEAKI 248

Query: 181 ---------WFLRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIETQ 225
                    W L DWNDEESI+ILK C+ AIP+RAE GKVI+I+MV++NQIEDKES+E Q
Sbjct: 249 PPANAVLLKWILHDWNDEESIRILKNCKDAIPSRAEXGKVIVIDMVVENQIEDKESVEAQ 308

BLAST of MC05g_new0176 vs. ExPASy TrEMBL
Match: A0A6J1DFY3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111020151 PE=3 SV=1)

HSP 1 Score: 300 bits (767), Expect = 1.96e-98
Identity = 164/290 (56.55%), Postives = 186/290 (64.14%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDEN-EQEAKYTLTSPSRLLLRHNT---------------------- 60
           M LLVHSGFFA+TH EN EQE KY +T  SRLLLRHN                       
Sbjct: 69  MRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHHAMMASWET 128

Query: 61  ---WLCSSDERYSCPFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWV 120
              WLCS DE YS  FEMAN KS+W+Y+A+EP+F N F++ + +DSQLIGRIVT ECR V
Sbjct: 129 MSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTECREV 188

Query: 121 FEGLKSLVDVGGSRGFMAKAIVEAFPHITCTWF--------------------------- 180
           FEGLKS+VDVGG  G MAKAIVEAFPHIT   F                           
Sbjct: 189 FEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDMFEAK 248

Query: 181 ------------LRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIET 225
                       L +WNDEESIQILK C+ AI + AEGGKVIIIE+ ++ Q+EDKESIET
Sbjct: 249 IPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKESIET 308

BLAST of MC05g_new0176 vs. ExPASy TrEMBL
Match: A0A6J1J5U8 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481541 PE=3 SV=1)

HSP 1 Score: 272 bits (695), Expect = 1.38e-87
Identity = 153/292 (52.40%), Postives = 183/292 (62.67%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRHN------------------------ 60
           M LLVHSGFFA+TH        ++LT+PSRLLLR N                        
Sbjct: 69  MRLLVHSGFFAETHS-----GFFSLTTPSRLLLRQNHNAFDTTPFLLLLLSPVFMAPWET 128

Query: 61  --TWLCSSDERY-SCPFEMANKKSLWDYVAQEPD-FRNTFNQAMMFDSQLIGRIVTRECR 120
              WLCS D+ + S  FEMAN  ++WDY+AQ+P  F N F+QA++ DSQLIG++VT ECR
Sbjct: 129 MNDWLCSDDQDHNSTAFEMANGNTIWDYIAQQPSGFGNLFHQALIHDSQLIGKVVTSECR 188

Query: 121 WVFEGLKSLVDVGGSRGFMAKAIVEAFPHITCTWF------------------------- 180
            +FEGL SLVDVGG  G MAKAIVEAFPHI+C  F                         
Sbjct: 189 GMFEGLTSLVDVGGGGGAMAKAIVEAFPHISCIVFDLPQVVANQEPQQAKKNLNFIGGDM 248

Query: 181 ---------------LRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKES 224
                          L DW+DEESIQILK C+ AIP+RAEGGKVIIIE+V++ Q  DKES
Sbjct: 249 FEKIPQANALLLKFVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEIVLEEQ-RDKES 308

BLAST of MC05g_new0176 vs. ExPASy TrEMBL
Match: A0A6J1F479 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442010 PE=3 SV=1)

HSP 1 Score: 272 bits (695), Expect = 1.38e-87
Identity = 153/292 (52.40%), Postives = 181/292 (61.99%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRHN------------------------ 60
           M LLVHSGFFA+TH        ++LT+PSRLLLR N                        
Sbjct: 69  MRLLVHSGFFAETHS-----GFFSLTTPSRLLLRQNHNAFDTTPLLLLLTSPVFMAPWET 128

Query: 61  --TWLCSSDERY-SCPFEMANKKSLWDYVAQEPD-FRNTFNQAMMFDSQLIGRIVTRECR 120
              WLCS D+ + S  FEMAN  ++WDY+AQ+P  F N F+Q ++ DSQLIG++VT ECR
Sbjct: 129 MSNWLCSDDKDHNSTAFEMANGNTIWDYIAQQPSGFGNLFHQTLIHDSQLIGKVVTSECR 188

Query: 121 WVFEGLKSLVDVGGSRGFMAKAIVEAFPHITCTWF------------------------- 180
            +FEGL SLVDVGG  G MAKAIVEAFPHI+C  F                         
Sbjct: 189 GMFEGLTSLVDVGGGAGAMAKAIVEAFPHISCIVFDLPQVVASQEPQQAKKNLNFIGGDM 248

Query: 181 ---------------LRDWNDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKES 224
                          L DW+DEESIQILK C+ AIP+RAEGGKVIIIEMV + Q  DKES
Sbjct: 249 FEKIPQANALLLKLVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEMVFEEQ-RDKES 308

BLAST of MC05g_new0176 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.1e-21
Identity = 62/215 (28.84%), Postives = 108/215 (50.23%), Query Frame = 0

Query: 50  FEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVFEGLKSLVDVGGSRG 109
           +E AN+  +++Y+ +  + +  FN++M   + ++ + +  E    FEG+   VDVGGS G
Sbjct: 152 WERANEALIFEYMKKNENLKKIFNESMTNHTSIVMKKIL-ENYIGFEGVSDFVDVGGSLG 211

Query: 110 FMAKAIVEAFPH-------------------------------------ITCTWFLRDWN 169
                I+  +PH                                     I   W L DWN
Sbjct: 212 SNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWN 271

Query: 170 DEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKE-SIETQLCDDVFMMSILT-GK 226
           DE+ ++ILK C+ A+P   E G++I+IEM++  ++ + + + +  L  D+ MMS+ + GK
Sbjct: 272 DEKCVEILKNCKKALP---ETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGK 331

BLAST of MC05g_new0176 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.1e-21
Identity = 62/215 (28.84%), Postives = 108/215 (50.23%), Query Frame = 0

Query: 50  FEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVFEGLKSLVDVGGSRG 109
           +E AN+  +++Y+ +  + +  FN++M   + ++ + +  E    FEG+   VDVGGS G
Sbjct: 152 WERANEALIFEYMKKNENLKKIFNESMTNHTSIVMKKIL-ENYIGFEGVSDFVDVGGSLG 211

Query: 110 FMAKAIVEAFPH-------------------------------------ITCTWFLRDWN 169
                I+  +PH                                     I   W L DWN
Sbjct: 212 SNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWN 271

Query: 170 DEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKE-SIETQLCDDVFMMSILT-GK 226
           DE+ ++ILK C+ A+P   E G++I+IEM++  ++ + + + +  L  D+ MMS+ + GK
Sbjct: 272 DEKCVEILKNCKKALP---ETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGK 331

BLAST of MC05g_new0176 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.1e-21
Identity = 62/221 (28.05%), Postives = 98/221 (44.34%), Query Frame = 0

Query: 49  PFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVFEGLKSLVDVGGSR 108
           PF+  + K +W +    P   +  N+AM  D++ +   V   C  +F+G+ ++VDVGG  
Sbjct: 162 PFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVPRVAGACHGLFDGVTTMVDVGGGT 221

Query: 109 GFMAKAIVEAFPHIT-------------------------------------CTWFLRDW 168
           G     +V+ FP I                                        W L DW
Sbjct: 222 GETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGDMFDSIPACDAIFIKWVLHDW 281

Query: 169 NDEESIQILKKCRYAIPNRAEGGKVIIIEMVM----KNQIEDKESIETQ----LCDDVFM 225
            D++ I+ILK C+ A+P     GKV+I+E V+    K  I D+   + +    + D V M
Sbjct: 282 GDKDCIKILKNCKEAVPPNI--GKVLIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMM 341

BLAST of MC05g_new0176 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 95.5 bits (236), Expect = 5.8e-20
Identity = 76/271 (28.04%), Postives = 114/271 (42.07%), Query Frame = 0

Query: 1   MHLLVHSGFFAQTHDENEQEAKYTLTSPSRLLLRHNTWLCSSDERYSCPFEMANKKSLWD 60
           M  LVH G F +   ++     YT T  SR ++                    + K LW 
Sbjct: 73  MRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM----------------ITKLHGKDLWA 132

Query: 61  YVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVFEGLKSLVDVGGSRGFMAKAIVEAFP 120
           +           N+AM  D++ +   V   C+ +F+G+ ++VDVGG  G     +V+ FP
Sbjct: 133 FAQDNLCHSQLINEAMACDARRVVPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFP 192

Query: 121 HI-------------------------------------TCTWFLRDWNDEESIQILKKC 180
            I                                        W L DW D++ I+ILK C
Sbjct: 193 WIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNC 252

Query: 181 RYAI-PNRAEGGKVIIIEMVM---KNQI-----EDK-ESIETQLCDDVFMMSILTGKERN 225
           + A+ PN    GKV+I+E V+   KN +     +DK E +  QL D V M+   TGKER 
Sbjct: 253 KEAVLPNI---GKVLIVECVIGEKKNTMIAEERDDKLEHVRLQL-DMVMMVHTSTGKERT 312

BLAST of MC05g_new0176 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 82.8 bits (203), Expect = 3.9e-16
Identity = 60/213 (28.17%), Postives = 91/213 (42.72%), Query Frame = 0

Query: 49  PFEMANKKSLWDYVAQEPDFRNTFNQAMMFDSQLIGRIVTRECRWVFEGLKSLVDVGGSR 108
           PF  A   S ++Y   +P F   FN  M   S +  + +    +  FEGL SLVDVGG  
Sbjct: 151 PFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGI 210

Query: 109 GFMAKAIVEAFPH-------------------------------------ITCTWFLRDW 168
           G   K IV  +P+                                     I   W   DW
Sbjct: 211 GATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDW 270

Query: 169 NDEESIQILKKCRYAIPNRAEGGKVIIIEMVMKNQIEDKESIETQLCDDVFMMSILT-GK 224
           +DE  ++ LK C  ++P   E GKVI+ E ++    +   S +  +  D  M++    GK
Sbjct: 271 SDEHCVKFLKNCYESLP---EDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGK 330