Homology
BLAST of MC09g0712 vs. ExPASy Swiss-Prot
Match:
Q94KE2 (Protein TIME FOR COFFEE OS=Arabidopsis thaliana OX=3702 GN=TIC PE=1 SV=2)
HSP 1 Score: 296.6 bits (758), Expect = 1.3e-78
Identity = 427/1447 (29.51%), Postives = 629/1447 (43.47%), Query Frame = 0
Query: 3 MEKTREARSSSSTVAANGSSRRRSRATVFRDLP-EEGQVELQETVRLRDK-GAGKRDRDR 62
M++ REAR A NG SRRR RA FRD P EEG VEL E RLRD+ G+ K+DRDR
Sbjct: 1 MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDR 60
Query: 63 E-----------------FASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVE 122
E SRSKRRRG D+GGD S+E+ V D +E +
Sbjct: 61 ERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESV---NDDEEYD 120
Query: 123 DGG-----------GVSRILSSATASSA-------------------SNQNLRMNSLPP- 182
DGG S +S+A+ SS+ +N + R N+ PP
Sbjct: 121 DGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPT 180
Query: 183 RVAKQQ------------WKVVDDAMIGVPVPRKAR--------------TGNSGVGGAG 242
+V + WK D+ MIGV VPRKAR T GV +G
Sbjct: 181 KVFRSSPSPAPVSPLVSTWKAADE-MIGVSVPRKARSACTKRPHESWASSTTGGGVFASG 240
Query: 243 EDIADDPSHRNPSD---------SPARSSAEVVSPSIFAKKKMKPTGPKTRTM----KAS 302
E I S +P++ SP+ + S S + +K P+G K + + +S
Sbjct: 241 EQIHRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPPKSSSS 300
Query: 303 KDSSP-STEGDIEIEIAEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVT 362
K SSP + + +IEIEIAEVL+G+ + P S+++ + N TE + + E KS V+
Sbjct: 301 KLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQE-----AAGNDLTEAAKSTVE-VKSRVS 360
Query: 363 STTANSAQTSFQKSVSLQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESE 422
S +N QT Q S++L N + S++ +A ++ K H D+ + +SE
Sbjct: 361 SPISN-PQTLPQSSITLAANSSSSNVSA-IAPKRKKPRHVK----YEDDNSSRVTTIKSE 420
Query: 423 QHAGIEGASDFGMNLGSHGCQTVVTKPEES--KLTKEFFTGGDENPGSKKTVSTQDDLPS 482
A + F L S G + +S LT+E D + LP
Sbjct: 421 AEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESNASLDSEKKENNLSKDETILPK 480
Query: 483 CIKGDVGPEDSPPSKSIPEAETQKEEKFKFDLMAPP---TSPERDG--LTDMVLDIKPLS 542
D +KS + ++EKF+ DLMAPP +S ER G + + + KP
Sbjct: 481 VESSSGFRSDGEGAKS----SSPEKEKFEIDLMAPPPVRSSSERGGEMMECVAAEAKPKV 540
Query: 543 LGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVVSSGKKFDF-----FKLDLERPQLDS 602
+E K + + + ++ R ++V+ + F KLDL++ D
Sbjct: 541 TEVETEAKPLLKEDRSDPAIHDSQ---EKKRPRMVAEAEHHKFERNCELKLDLDKS--DH 600
Query: 603 NSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWPTAEIPPVGYMPPFRTMLSVD 662
L + QK + +K ++ + L +++ GWP +P +GYM P + ++ D
Sbjct: 601 VGLVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWP-GGLPTMGYMAPTQGVVPTD 660
Query: 663 SGDKSSSKLQ-HPNFILSHPRSKRCLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEA 722
+ S++ +Q P+ + + PR KRC TH IARNI HQQFTK N F PAA S +
Sbjct: 661 TSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGSAPMYGT 720
Query: 723 KLKNLSSKEGISL-GNPLHRHLLDMNLNTAQAEGDFPGNVVNDK-SPEAANFADMAKSKQ 782
K NLS L G+ L R N V DK S + ++ A+ Q
Sbjct: 721 KACNLSLMPPTELQGSVLGR----------------SSNPVQDKNSQSTSKSSETAQRNQ 780
Query: 783 LVFQQRPGPSFGNS-MPGSGFIFPISQ--HQASIAATAN----QSGTTKSSNQSTSLFSN 842
L+ QQ P NS + G FIFP+ Q H A+ A A+ SG T S +TS N
Sbjct: 781 LMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGPTATSTSMN 840
Query: 843 PEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPAGTSTTYRANS 902
A A PA + MS+S+P M E YLA LQNNGYPF G YR
Sbjct: 841 GSAS------ATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGAQPAYRGAP 900
Query: 903 AQPLPLFNGSFYPSQLFHPSQIQPAQ----------TQSHHQ----------SSSSHKQP 962
QP+P FNGSFY SQ+ P QP + QSH SS++ K
Sbjct: 901 GQPMPFFNGSFYSSQMIQPPHHQPQKQHQQQLTGQMLQSHAPNNQNGSASTGSSAAQKHL 960
Query: 963 QAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAP 1022
Q QQ + ++ + P++ +Q + Q ++ ET GED+ + +D R
Sbjct: 961 QNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSPSTADSR-GS 1020
Query: 1023 VQKNTYGQNFILPVQALGFTLMPSATMNN---ANGGNNGEKKQSHPQNLKNTVALVPSQG 1082
YGQN+ + +Q LM S + ++GEKK S Q K V S G
Sbjct: 1021 RSNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKK-SQQQVSKAGVESFQSPG 1080
Query: 1083 LTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSS--E 1142
M+FA++NG T LN + IAQN ++ ++P+ R G+Q+ A A ++ ++ +S +
Sbjct: 1081 YAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKMNYGASLED 1140
Query: 1143 GRNGVVLSGSNDRNNGNSAKPS----------STSGQTLVFSNSGRTLNFMTSSAPVNWP 1202
G++G + + N + S GQ++ FSN + L + SA +
Sbjct: 1141 GKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNK-QDLADASVSAVTSGS 1200
Query: 1203 SPSTKSAALTTNRSAASSSG----NQQQQPLLQLPQQHI-------------LQQQQ--- 1250
+ S L + SSG + QQ L Q QQH+ LQ+QQ
Sbjct: 1201 IVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYLQKQQRYA 1260
BLAST of MC09g0712 vs. NCBI nr
Match:
XP_022150282.1 (protein TIME FOR COFFEE isoform X2 [Momordica charantia])
HSP 1 Score: 2397 bits (6212), Expect = 0.0
Identity = 1263/1263 (100.00%), Postives = 1263/1263 (100.00%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR
Sbjct: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA
Sbjct: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
Query: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNPSD 180
SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNPSD
Sbjct: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNPSD 180
Query: 181 SPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEIAEVLFGLKKQPQ 240
SPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEIAEVLFGLKKQPQ
Sbjct: 181 SPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEIAEVLFGLKKQPQ 240
Query: 241 CSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGAISDLLL 300
CSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGAISDLLL
Sbjct: 241 CSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGAISDLLL 300
Query: 301 NVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQTVVTKPE 360
NVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQTVVTKPE
Sbjct: 301 NVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQTVVTKPE 360
Query: 361 ESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQKEEKFKF 420
ESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQKEEKFKF
Sbjct: 361 ESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQKEEKFKF 420
Query: 421 DLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVV 480
DLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVV
Sbjct: 421 DLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVV 480
Query: 481 SSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWP 540
SSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWP
Sbjct: 481 SSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWP 540
Query: 541 TAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHYDIARNIYLHQQF 600
TAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHYDIARNIYLHQQF
Sbjct: 541 TAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHYDIARNIYLHQQF 600
Query: 601 TKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTAQAEGDFPGNVVN 660
TKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTAQAEGDFPGNVVN
Sbjct: 601 TKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTAQAEGDFPGNVVN 660
Query: 661 DKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASIAATANQSGTTKS 720
DKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASIAATANQSGTTKS
Sbjct: 661 DKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASIAATANQSGTTKS 720
Query: 721 SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPA 780
SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPA
Sbjct: 721 SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPA 780
Query: 781 GTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHKQPQAQQQQWSVH 840
GTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHKQPQAQQQQWSVH
Sbjct: 781 GTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHKQPQAQQQQWSVH 840
Query: 841 VPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAPVQKNTYGQNF 900
VPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAPVQKNTYGQNF
Sbjct: 841 VPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAPVQKNTYGQNF 900
Query: 901 ILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGLTMSFASYNGNGT 960
ILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGLTMSFASYNGNGT
Sbjct: 901 ILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGLTMSFASYNGNGT 960
Query: 961 PSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRNGVVLSGSNDRNN 1020
PSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRNGVVLSGSNDRNN
Sbjct: 961 PSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRNGVVLSGSNDRNN 1020
Query: 1021 GNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNRSAASSSGNQQQQ 1080
GNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNRSAASSSGNQQQQ
Sbjct: 1021 GNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNRSAASSSGNQQQQ 1080
Query: 1081 PLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQPLMHCDDSAQKN 1140
PLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQPLMHCDDSAQKN
Sbjct: 1081 PLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQPLMHCDDSAQKN 1140
Query: 1141 SGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSS 1200
SGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSS
Sbjct: 1141 SGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSS 1200
Query: 1201 HSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSE 1260
HSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSE
Sbjct: 1201 HSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSE 1260
Query: 1261 LKY 1263
LKY
Sbjct: 1261 LKY 1263
BLAST of MC09g0712 vs. NCBI nr
Match:
XP_022150281.1 (protein TIME FOR COFFEE isoform X1 [Momordica charantia])
HSP 1 Score: 2388 bits (6189), Expect = 0.0
Identity = 1263/1275 (99.06%), Postives = 1263/1275 (99.06%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR
Sbjct: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA
Sbjct: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
Query: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKART------------GNSGVGGAGED 180
SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKART GNSGVGGAGED
Sbjct: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTASVKRSHDCTVSGNSGVGGAGED 180
Query: 181 IADDPSHRNPSDSPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEI 240
IADDPSHRNPSDSPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEI
Sbjct: 181 IADDPSHRNPSDSPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEI 240
Query: 241 AEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVS 300
AEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVS
Sbjct: 241 AEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVS 300
Query: 301 LQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLG 360
LQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLG
Sbjct: 301 LQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLG 360
Query: 361 SHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIP 420
SHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIP
Sbjct: 361 SHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIP 420
Query: 421 EAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEK 480
EAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEK
Sbjct: 421 EAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEK 480
Query: 481 EKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPST 540
EKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPST
Sbjct: 481 EKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPST 540
Query: 541 SVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHY 600
SVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHY
Sbjct: 541 SVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHY 600
Query: 601 DIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTA 660
DIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTA
Sbjct: 601 DIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTA 660
Query: 661 QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASI 720
QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASI
Sbjct: 661 QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASI 720
Query: 721 AATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQ 780
AATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQ
Sbjct: 721 AATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQ 780
Query: 781 NNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHK 840
NNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHK
Sbjct: 781 NNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHK 840
Query: 841 QPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRV 900
QPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRV
Sbjct: 841 QPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRV 900
Query: 901 APVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGL 960
APVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGL
Sbjct: 901 APVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGL 960
Query: 961 TMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRN 1020
TMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRN
Sbjct: 961 TMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRN 1020
Query: 1021 GVVLSGSNDRNNGNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNR 1080
GVVLSGSNDRNNGNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNR
Sbjct: 1021 GVVLSGSNDRNNGNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNR 1080
Query: 1081 SAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQ 1140
SAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQ
Sbjct: 1081 SAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQ 1140
Query: 1141 PLMHCDDSAQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKP 1200
PLMHCDDSAQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKP
Sbjct: 1141 PLMHCDDSAQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKP 1200
Query: 1201 AFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVP 1260
AFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVP
Sbjct: 1201 AFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVP 1260
Query: 1261 SILNTCPSHLSELKY 1263
SILNTCPSHLSELKY
Sbjct: 1261 SILNTCPSHLSELKY 1275
BLAST of MC09g0712 vs. NCBI nr
Match:
XP_038896876.1 (protein TIME FOR COFFEE-like isoform X3 [Benincasa hispida])
HSP 1 Score: 1811 bits (4692), Expect = 0.0
Identity = 1019/1283 (79.42%), Postives = 1089/1283 (84.88%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEK REARSSSS VAANGSSRRRSRAT FRDLPEEGQVELQETVRLRD+G GKRDRDR
Sbjct: 1 MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRG-GKRDRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSAT-ASS 120
EFASRSKRRRGG NREEEVEE EGGDTSAED+V DG DS EVEDGGGVSRILSS+T ASS
Sbjct: 61 EFASRSKRRRGGGNREEEVEE-EGGDTSAEDIVADG-DSYEVEDGGGVSRILSSSTTASS 120
Query: 121 ASNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNPS 180
SNQN + NSLPPRVAKQQWKVVDDAMIGVPVPRKAR+GNSGVGGAGEDIADD SHRN S
Sbjct: 121 VSNQNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKARSGNSGVGGAGEDIADDHSHRNQS 180
Query: 181 DSPARSSAEVVSPS---IFAKKKMKPTGPKTRTMKASKDSSPST-EGDIEIEIAEVLFGL 240
DSPARSSAEVVSPS I AKKKMK TGPKTR+MK SKDSS S EGDIEIEIAEVLFGL
Sbjct: 181 DSPARSSAEVVSPSTSIISAKKKMKSTGPKTRSMKTSKDSSRSAKEGDIEIEIAEVLFGL 240
Query: 241 KKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGAI 300
KKQPQCS+KQE+ K SS QETENSS LR+ +KS VTSTTANS+ T+FQKS SLQKN AI
Sbjct: 241 KKQPQCSKKQEVNVKQSSKQETENSSVLRDASKSSVTSTTANSSPTAFQKSASLQKNCAI 300
Query: 301 SDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQTV 360
SDL LNVA EK K + S S+ G AKGESE+ A +E +
Sbjct: 301 SDLSLNVAGEKKKVD---------SSSLDGTAKGESEKPAEVEIYPS-----------KL 360
Query: 361 VTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQKE 420
EESK KE TGGDEN GSK+T +D SC KGDV PEDS ++SIPEA QKE
Sbjct: 361 EVASEESKPAKEVCTGGDENKGSKRTGLAPEDANSCAKGDVDPEDSLRTRSIPEAVNQKE 420
Query: 421 EKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEKEKVIDEV 480
EKFKFDLMAPPTSPERDGLTDMV D KPLSLG+EMGK TS+KVENEVEGFKEKEKVIDE
Sbjct: 421 EKFKFDLMAPPTSPERDGLTDMVPDAKPLSLGIEMGKETSNKVENEVEGFKEKEKVIDE- 480
Query: 481 RDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPIT 540
DK+V+SG KF+FFKLDLE+PQLDSNS+ MQEQSQKQQ KGAA TVEKNEPSTSVRLPI
Sbjct: 481 -DKIVTSGTKFEFFKLDLEKPQLDSNSIAMQEQSQKQQPKGAASTVEKNEPSTSVRLPII 540
Query: 541 LGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHYDIARNIY 600
LGGWPT EIP VGYMPPFRT+L VDS DKSS+KLQHPNFILS+PR KRCLTHYD+ARNIY
Sbjct: 541 LGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHPNFILSYPRPKRCLTHYDVARNIY 600
Query: 601 LHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTAQ----AE 660
LHQQFTKTNYFHPA DAS SLAEAKLKN+SSKEG+ L NPL + LD+NLN+ Q AE
Sbjct: 601 LHQQFTKTNYFHPAGDASASLAEAKLKNMSSKEGMLLSNPLTGNHLDVNLNSVQQREQAE 660
Query: 661 GDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSM-PGSGFIFPISQHQASIAA 720
GDFPGNVVNDKSPEAANFAD+AKSKQLVFQQRPG FGNSM PGSGFIFPI QHQA IAA
Sbjct: 661 GDFPGNVVNDKSPEAANFADIAKSKQLVFQQRPGAPFGNSMQPGSGFIFPIGQHQAPIAA 720
Query: 721 -TANQSGTTKS-SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQ 780
TANQSG+ KS +NQSTSLF+NPEAGTLVSFPA P VS+NMSYSHPN+VS+EAPYLAKLQ
Sbjct: 721 ATANQSGSAKSPNNQSTSLFNNPEAGTLVSFPAFPPVSTNMSYSHPNVVSVEAPYLAKLQ 780
Query: 781 NNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHK 840
+NGYPFTFSTPAGTS TYRA++AQPLPLFNGSFYPSQLFHPSQIQPAQTQ+HHQ S SHK
Sbjct: 781 SNGYPFTFSTPAGTSATYRASNAQPLPLFNGSFYPSQLFHPSQIQPAQTQTHHQPSGSHK 840
Query: 841 QPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRV 900
QPQ QQQQWSVHVPGNN++PSN MQVKQSTEQHLPLSNQSRKH+ ET GEDT +LSDKR
Sbjct: 841 QPQTQQQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSNQSRKHENETSGEDTTSLSDKRA 900
Query: 901 APVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGL 960
A VQKN YGQN+ILPVQALGFTLMPSATMNN NGG +GEKKQSH QNLK+TVALVPSQGL
Sbjct: 901 ASVQKNAYGQNYILPVQALGFTLMPSATMNNGNGGYHGEKKQSHSQNLKSTVALVPSQGL 960
Query: 961 TMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRN 1020
TMSFASYNGNGTPSNLNFTPIAQN TVYQNLPD+QR GFQVAP PQATKQK+HQ+SEGRN
Sbjct: 961 TMSFASYNGNGTPSNLNFTPIAQNTTVYQNLPDIQRTGFQVAPTPQATKQKNHQTSEGRN 1020
Query: 1021 GVVLSGSNDRNNGNSA-KPSS-TSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTT 1080
GV+LSG++DRN GNS+ KPS+ TSGQTLVF NS RTLNFMTSS PVNWPS STKSAA TT
Sbjct: 1021 GVILSGTSDRNTGNSSTKPSTATSGQTLVFGNSSRTLNFMTSSVPVNWPSHSTKSAASTT 1080
Query: 1081 NRSAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGF 1140
NR A SSSGNQQQQ + + M RTKVP NN+LPSS KFP NAPPGF
Sbjct: 1081 NRPAGSSSGNQQQQATMA-----------SRMAPRTKVPTNNTLPSSATTKFPCNAPPGF 1140
Query: 1141 SQPLMHCDDS----AQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISF 1200
SQPL+ CD+S AQKNSGR+VASVVPMTSLHLASS AV KNS QQKGS QGQTQISF
Sbjct: 1141 SQPLIQCDNSIQSPAQKNSGRLVASVVPMTSLHLASSAAVHKNSIQQKGSNTQGQTQISF 1200
Query: 1201 GG-DFKPAFTQMQHIPTSSHSPSSSGKLRNT-GSKTSSSVAPPQPKQDESSSTGGGQKSS 1260
GG DFKPA+TQMQHIPTS HSPSSSGKLRN SKTS SV P QPKQDESSSTG GQKSS
Sbjct: 1201 GGGDFKPAYTQMQHIPTSGHSPSSSGKLRNNISSKTSPSVTPTQPKQDESSSTGAGQKSS 1247
Query: 1261 PVCGRNVPSILNTCPSHLSELKY 1263
PVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 PVCGRNVPSILNTCPSHLSELKY 1247
BLAST of MC09g0712 vs. NCBI nr
Match:
XP_038896875.1 (protein TIME FOR COFFEE-like isoform X2 [Benincasa hispida])
HSP 1 Score: 1807 bits (4681), Expect = 0.0
Identity = 1019/1294 (78.75%), Postives = 1089/1294 (84.16%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEK REARSSSS VAANGSSRRRSRAT FRDLPEEGQVELQETVRLRD+G GKRDRDR
Sbjct: 1 MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRG-GKRDRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSAT-ASS 120
EFASRSKRRRGG NREEEVEE EGGDTSAED+V DG DS EVEDGGGVSRILSS+T ASS
Sbjct: 61 EFASRSKRRRGGGNREEEVEE-EGGDTSAEDIVADG-DSYEVEDGGGVSRILSSSTTASS 120
Query: 121 ASNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKART------------GNSGVGGAGE 180
SNQN + NSLPPRVAKQQWKVVDDAMIGVPVPRKAR+ GNSGVGGAGE
Sbjct: 121 VSNQNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGE 180
Query: 181 DIADDPSHRNPSDSPARSSAEVVSPS---IFAKKKMKPTGPKTRTMKASKDSSPST-EGD 240
DIADD SHRN SDSPARSSAEVVSPS I AKKKMK TGPKTR+MK SKDSS S EGD
Sbjct: 181 DIADDHSHRNQSDSPARSSAEVVSPSTSIISAKKKMKSTGPKTRSMKTSKDSSRSAKEGD 240
Query: 241 IEIEIAEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSF 300
IEIEIAEVLFGLKKQPQCS+KQE+ K SS QETENSS LR+ +KS VTSTTANS+ T+F
Sbjct: 241 IEIEIAEVLFGLKKQPQCSKKQEVNVKQSSKQETENSSVLRDASKSSVTSTTANSSPTAF 300
Query: 301 QKSVSLQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDF 360
QKS SLQKN AISDL LNVA EK K + S S+ G AKGESE+ A +E
Sbjct: 301 QKSASLQKNCAISDLSLNVAGEKKKVD---------SSSLDGTAKGESEKPAEVEIYPS- 360
Query: 361 GMNLGSHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPP 420
+ EESK KE TGGDEN GSK+T +D SC KGDV PEDS
Sbjct: 361 ----------KLEVASEESKPAKEVCTGGDENKGSKRTGLAPEDANSCAKGDVDPEDSLR 420
Query: 421 SKSIPEAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVE 480
++SIPEA QKEEKFKFDLMAPPTSPERDGLTDMV D KPLSLG+EMGK TS+KVENEVE
Sbjct: 421 TRSIPEAVNQKEEKFKFDLMAPPTSPERDGLTDMVPDAKPLSLGIEMGKETSNKVENEVE 480
Query: 481 GFKEKEKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEK 540
GFKEKEKVIDE DK+V+SG KF+FFKLDLE+PQLDSNS+ MQEQSQKQQ KGAA TVEK
Sbjct: 481 GFKEKEKVIDE--DKIVTSGTKFEFFKLDLEKPQLDSNSIAMQEQSQKQQPKGAASTVEK 540
Query: 541 NEPSTSVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKR 600
NEPSTSVRLPI LGGWPT EIP VGYMPPFRT+L VDS DKSS+KLQHPNFILS+PR KR
Sbjct: 541 NEPSTSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHPNFILSYPRPKR 600
Query: 601 CLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDM 660
CLTHYD+ARNIYLHQQFTKTNYFHPA DAS SLAEAKLKN+SSKEG+ L NPL + LD+
Sbjct: 601 CLTHYDVARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNMSSKEGMLLSNPLTGNHLDV 660
Query: 661 NLNTAQ----AEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIF 720
NLN+ Q AEGDFPGNVVNDKSPEAANFAD+AKSKQLVFQQRPG FGNSMPGSGFIF
Sbjct: 661 NLNSVQQREQAEGDFPGNVVNDKSPEAANFADIAKSKQLVFQQRPGAPFGNSMPGSGFIF 720
Query: 721 PISQHQASIAA-TANQSGTTKS-SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMV 780
PI QHQA IAA TANQSG+ KS +NQSTSLF+NPEAGTLVSFPA P VS+NMSYSHPN+V
Sbjct: 721 PIGQHQAPIAAATANQSGSAKSPNNQSTSLFNNPEAGTLVSFPAFPPVSTNMSYSHPNVV 780
Query: 781 SIEAPYLAKLQNNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQT 840
S+EAPYLAKLQ+NGYPFTFSTPAGTS TYRA++AQPLPLFNGSFYPSQLFHPSQIQPAQT
Sbjct: 781 SVEAPYLAKLQSNGYPFTFSTPAGTSATYRASNAQPLPLFNGSFYPSQLFHPSQIQPAQT 840
Query: 841 QSHHQSSSSHKQPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGG 900
Q+HHQ S SHKQPQ QQQQWSVHVPGNN++PSN MQVKQSTEQHLPLSNQSRKH+ ET G
Sbjct: 841 QTHHQPSGSHKQPQTQQQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSNQSRKHENETSG 900
Query: 901 EDTAALSDKRVAPVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLK 960
EDT +LSDKR A VQKN YGQN+ILPVQALGFTLMPSATMNN NGG +GEKKQSH QNLK
Sbjct: 901 EDTTSLSDKRAASVQKNAYGQNYILPVQALGFTLMPSATMNNGNGGYHGEKKQSHSQNLK 960
Query: 961 NTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATK 1020
+TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQN TVYQNLPD+QR GFQVAP PQATK
Sbjct: 961 STVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTTVYQNLPDIQRTGFQVAPTPQATK 1020
Query: 1021 QKSHQSSEGRNGVVLSGSNDRNNGNSA-KPSS-TSGQTLVFSNSGRTLNFMTSSAPVNWP 1080
QK+HQ+SEGRNGV+LSG++DRN GNS+ KPS+ TSGQTLVF NS RTLNFMTSS PVNWP
Sbjct: 1021 QKNHQTSEGRNGVILSGTSDRNTGNSSTKPSTATSGQTLVFGNSSRTLNFMTSSVPVNWP 1080
Query: 1081 SPSTKSAALTTNRSAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVG 1140
S STKSAA TTNR A SSSGNQQQQ + + M RTKVP NN+LPSS
Sbjct: 1081 SHSTKSAASTTNRPAGSSSGNQQQQATMA-----------SRMAPRTKVPTNNTLPSSAT 1140
Query: 1141 PKFPYNAPPGFSQPLMHCDDS----AQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKG 1200
KFP NAPPGFSQPL+ CD+S AQKNSGR+VASVVPMTSLHLASS AV KNS QQKG
Sbjct: 1141 TKFPCNAPPGFSQPLIQCDNSIQSPAQKNSGRLVASVVPMTSLHLASSAAVHKNSIQQKG 1200
Query: 1201 STPQGQTQISFGG-DFKPAFTQMQHIPTSSHSPSSSGKLRNT-GSKTSSSVAPPQPKQDE 1260
S QGQTQISFGG DFKPA+TQMQHIPTS HSPSSSGKLRN SKTS SV P QPKQDE
Sbjct: 1201 SNTQGQTQISFGGGDFKPAYTQMQHIPTSGHSPSSSGKLRNNISSKTSPSVTPTQPKQDE 1258
Query: 1261 SSSTGGGQKSSPVCGRNVPSILNTCPSHLSELKY 1263
SSSTG GQKSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 SSSTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1258
BLAST of MC09g0712 vs. NCBI nr
Match:
XP_038896874.1 (protein TIME FOR COFFEE-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1803 bits (4669), Expect = 0.0
Identity = 1019/1295 (78.69%), Postives = 1089/1295 (84.09%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEK REARSSSS VAANGSSRRRSRAT FRDLPEEGQVELQETVRLRD+G GKRDRDR
Sbjct: 1 MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRG-GKRDRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSAT-ASS 120
EFASRSKRRRGG NREEEVEE EGGDTSAED+V DG DS EVEDGGGVSRILSS+T ASS
Sbjct: 61 EFASRSKRRRGGGNREEEVEE-EGGDTSAEDIVADG-DSYEVEDGGGVSRILSSSTTASS 120
Query: 121 ASNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKART------------GNSGVGGAGE 180
SNQN + NSLPPRVAKQQWKVVDDAMIGVPVPRKAR+ GNSGVGGAGE
Sbjct: 121 VSNQNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGE 180
Query: 181 DIADDPSHRNPSDSPARSSAEVVSPS---IFAKKKMKPTGPKTRTMKASKDSSPST-EGD 240
DIADD SHRN SDSPARSSAEVVSPS I AKKKMK TGPKTR+MK SKDSS S EGD
Sbjct: 181 DIADDHSHRNQSDSPARSSAEVVSPSTSIISAKKKMKSTGPKTRSMKTSKDSSRSAKEGD 240
Query: 241 IEIEIAEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSF 300
IEIEIAEVLFGLKKQPQCS+KQE+ K SS QETENSS LR+ +KS VTSTTANS+ T+F
Sbjct: 241 IEIEIAEVLFGLKKQPQCSKKQEVNVKQSSKQETENSSVLRDASKSSVTSTTANSSPTAF 300
Query: 301 QKSVSLQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDF 360
QKS SLQKN AISDL LNVA EK K + S S+ G AKGESE+ A +E
Sbjct: 301 QKSASLQKNCAISDLSLNVAGEKKKVD---------SSSLDGTAKGESEKPAEVEIYPS- 360
Query: 361 GMNLGSHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPP 420
+ EESK KE TGGDEN GSK+T +D SC KGDV PEDS
Sbjct: 361 ----------KLEVASEESKPAKEVCTGGDENKGSKRTGLAPEDANSCAKGDVDPEDSLR 420
Query: 421 SKSIPEAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVE 480
++SIPEA QKEEKFKFDLMAPPTSPERDGLTDMV D KPLSLG+EMGK TS+KVENEVE
Sbjct: 421 TRSIPEAVNQKEEKFKFDLMAPPTSPERDGLTDMVPDAKPLSLGIEMGKETSNKVENEVE 480
Query: 481 GFKEKEKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEK 540
GFKEKEKVIDE DK+V+SG KF+FFKLDLE+PQLDSNS+ MQEQSQKQQ KGAA TVEK
Sbjct: 481 GFKEKEKVIDE--DKIVTSGTKFEFFKLDLEKPQLDSNSIAMQEQSQKQQPKGAASTVEK 540
Query: 541 NEPSTSVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKR 600
NEPSTSVRLPI LGGWPT EIP VGYMPPFRT+L VDS DKSS+KLQHPNFILS+PR KR
Sbjct: 541 NEPSTSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHPNFILSYPRPKR 600
Query: 601 CLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDM 660
CLTHYD+ARNIYLHQQFTKTNYFHPA DAS SLAEAKLKN+SSKEG+ L NPL + LD+
Sbjct: 601 CLTHYDVARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNMSSKEGMLLSNPLTGNHLDV 660
Query: 661 NLNTAQ----AEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSM-PGSGFI 720
NLN+ Q AEGDFPGNVVNDKSPEAANFAD+AKSKQLVFQQRPG FGNSM PGSGFI
Sbjct: 661 NLNSVQQREQAEGDFPGNVVNDKSPEAANFADIAKSKQLVFQQRPGAPFGNSMQPGSGFI 720
Query: 721 FPISQHQASIAA-TANQSGTTKS-SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNM 780
FPI QHQA IAA TANQSG+ KS +NQSTSLF+NPEAGTLVSFPA P VS+NMSYSHPN+
Sbjct: 721 FPIGQHQAPIAAATANQSGSAKSPNNQSTSLFNNPEAGTLVSFPAFPPVSTNMSYSHPNV 780
Query: 781 VSIEAPYLAKLQNNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQ 840
VS+EAPYLAKLQ+NGYPFTFSTPAGTS TYRA++AQPLPLFNGSFYPSQLFHPSQIQPAQ
Sbjct: 781 VSVEAPYLAKLQSNGYPFTFSTPAGTSATYRASNAQPLPLFNGSFYPSQLFHPSQIQPAQ 840
Query: 841 TQSHHQSSSSHKQPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETG 900
TQ+HHQ S SHKQPQ QQQQWSVHVPGNN++PSN MQVKQSTEQHLPLSNQSRKH+ ET
Sbjct: 841 TQTHHQPSGSHKQPQTQQQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSNQSRKHENETS 900
Query: 901 GEDTAALSDKRVAPVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNL 960
GEDT +LSDKR A VQKN YGQN+ILPVQALGFTLMPSATMNN NGG +GEKKQSH QNL
Sbjct: 901 GEDTTSLSDKRAASVQKNAYGQNYILPVQALGFTLMPSATMNNGNGGYHGEKKQSHSQNL 960
Query: 961 KNTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQAT 1020
K+TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQN TVYQNLPD+QR GFQVAP PQAT
Sbjct: 961 KSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTTVYQNLPDIQRTGFQVAPTPQAT 1020
Query: 1021 KQKSHQSSEGRNGVVLSGSNDRNNGNSA-KPSS-TSGQTLVFSNSGRTLNFMTSSAPVNW 1080
KQK+HQ+SEGRNGV+LSG++DRN GNS+ KPS+ TSGQTLVF NS RTLNFMTSS PVNW
Sbjct: 1021 KQKNHQTSEGRNGVILSGTSDRNTGNSSTKPSTATSGQTLVFGNSSRTLNFMTSSVPVNW 1080
Query: 1081 PSPSTKSAALTTNRSAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSV 1140
PS STKSAA TTNR A SSSGNQQQQ + + M RTKVP NN+LPSS
Sbjct: 1081 PSHSTKSAASTTNRPAGSSSGNQQQQATMA-----------SRMAPRTKVPTNNTLPSSA 1140
Query: 1141 GPKFPYNAPPGFSQPLMHCDDS----AQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQK 1200
KFP NAPPGFSQPL+ CD+S AQKNSGR+VASVVPMTSLHLASS AV KNS QQK
Sbjct: 1141 TTKFPCNAPPGFSQPLIQCDNSIQSPAQKNSGRLVASVVPMTSLHLASSAAVHKNSIQQK 1200
Query: 1201 GSTPQGQTQISFGG-DFKPAFTQMQHIPTSSHSPSSSGKLRNT-GSKTSSSVAPPQPKQD 1260
GS QGQTQISFGG DFKPA+TQMQHIPTS HSPSSSGKLRN SKTS SV P QPKQD
Sbjct: 1201 GSNTQGQTQISFGGGDFKPAYTQMQHIPTSGHSPSSSGKLRNNISSKTSPSVTPTQPKQD 1259
Query: 1261 ESSSTGGGQKSSPVCGRNVPSILNTCPSHLSELKY 1263
ESSSTG GQKSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 ESSSTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1259
BLAST of MC09g0712 vs. ExPASy TrEMBL
Match:
A0A6J1DAB7 (protein TIME FOR COFFEE isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018485 PE=4 SV=1)
HSP 1 Score: 2397 bits (6212), Expect = 0.0
Identity = 1263/1263 (100.00%), Postives = 1263/1263 (100.00%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR
Sbjct: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA
Sbjct: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
Query: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNPSD 180
SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNPSD
Sbjct: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNPSD 180
Query: 181 SPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEIAEVLFGLKKQPQ 240
SPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEIAEVLFGLKKQPQ
Sbjct: 181 SPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEIAEVLFGLKKQPQ 240
Query: 241 CSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGAISDLLL 300
CSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGAISDLLL
Sbjct: 241 CSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGAISDLLL 300
Query: 301 NVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQTVVTKPE 360
NVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQTVVTKPE
Sbjct: 301 NVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQTVVTKPE 360
Query: 361 ESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQKEEKFKF 420
ESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQKEEKFKF
Sbjct: 361 ESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQKEEKFKF 420
Query: 421 DLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVV 480
DLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVV
Sbjct: 421 DLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVV 480
Query: 481 SSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWP 540
SSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWP
Sbjct: 481 SSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWP 540
Query: 541 TAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHYDIARNIYLHQQF 600
TAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHYDIARNIYLHQQF
Sbjct: 541 TAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHYDIARNIYLHQQF 600
Query: 601 TKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTAQAEGDFPGNVVN 660
TKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTAQAEGDFPGNVVN
Sbjct: 601 TKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTAQAEGDFPGNVVN 660
Query: 661 DKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASIAATANQSGTTKS 720
DKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASIAATANQSGTTKS
Sbjct: 661 DKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASIAATANQSGTTKS 720
Query: 721 SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPA 780
SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPA
Sbjct: 721 SNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPA 780
Query: 781 GTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHKQPQAQQQQWSVH 840
GTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHKQPQAQQQQWSVH
Sbjct: 781 GTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHKQPQAQQQQWSVH 840
Query: 841 VPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAPVQKNTYGQNF 900
VPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAPVQKNTYGQNF
Sbjct: 841 VPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAPVQKNTYGQNF 900
Query: 901 ILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGLTMSFASYNGNGT 960
ILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGLTMSFASYNGNGT
Sbjct: 901 ILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGLTMSFASYNGNGT 960
Query: 961 PSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRNGVVLSGSNDRNN 1020
PSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRNGVVLSGSNDRNN
Sbjct: 961 PSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRNGVVLSGSNDRNN 1020
Query: 1021 GNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNRSAASSSGNQQQQ 1080
GNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNRSAASSSGNQQQQ
Sbjct: 1021 GNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNRSAASSSGNQQQQ 1080
Query: 1081 PLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQPLMHCDDSAQKN 1140
PLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQPLMHCDDSAQKN
Sbjct: 1081 PLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQPLMHCDDSAQKN 1140
Query: 1141 SGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSS 1200
SGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSS
Sbjct: 1141 SGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSS 1200
Query: 1201 HSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSE 1260
HSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSE
Sbjct: 1201 HSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSE 1260
Query: 1261 LKY 1263
LKY
Sbjct: 1261 LKY 1263
BLAST of MC09g0712 vs. ExPASy TrEMBL
Match:
A0A6J1DB26 (protein TIME FOR COFFEE isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018485 PE=4 SV=1)
HSP 1 Score: 2388 bits (6189), Expect = 0.0
Identity = 1263/1275 (99.06%), Postives = 1263/1275 (99.06%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR
Sbjct: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA
Sbjct: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
Query: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKART------------GNSGVGGAGED 180
SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKART GNSGVGGAGED
Sbjct: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTASVKRSHDCTVSGNSGVGGAGED 180
Query: 181 IADDPSHRNPSDSPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEI 240
IADDPSHRNPSDSPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEI
Sbjct: 181 IADDPSHRNPSDSPARSSAEVVSPSIFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEI 240
Query: 241 AEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVS 300
AEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVS
Sbjct: 241 AEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVS 300
Query: 301 LQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLG 360
LQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLG
Sbjct: 301 LQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLG 360
Query: 361 SHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIP 420
SHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIP
Sbjct: 361 SHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIP 420
Query: 421 EAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEK 480
EAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEK
Sbjct: 421 EAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEK 480
Query: 481 EKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPST 540
EKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPST
Sbjct: 481 EKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPST 540
Query: 541 SVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHY 600
SVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHY
Sbjct: 541 SVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHY 600
Query: 601 DIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTA 660
DIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTA
Sbjct: 601 DIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTA 660
Query: 661 QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASI 720
QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASI
Sbjct: 661 QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPISQHQASI 720
Query: 721 AATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQ 780
AATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQ
Sbjct: 721 AATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQ 780
Query: 781 NNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHK 840
NNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHK
Sbjct: 781 NNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQSSSSHK 840
Query: 841 QPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRV 900
QPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRV
Sbjct: 841 QPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRV 900
Query: 901 APVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGL 960
APVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGL
Sbjct: 901 APVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVALVPSQGL 960
Query: 961 TMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRN 1020
TMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRN
Sbjct: 961 TMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSSEGRN 1020
Query: 1021 GVVLSGSNDRNNGNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNR 1080
GVVLSGSNDRNNGNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNR
Sbjct: 1021 GVVLSGSNDRNNGNSAKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKSAALTTNR 1080
Query: 1081 SAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQ 1140
SAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQ
Sbjct: 1081 SAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYNAPPGFSQ 1140
Query: 1141 PLMHCDDSAQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKP 1200
PLMHCDDSAQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKP
Sbjct: 1141 PLMHCDDSAQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKGSTPQGQTQISFGGDFKP 1200
Query: 1201 AFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVP 1260
AFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVP
Sbjct: 1201 AFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGGQKSSPVCGRNVP 1260
Query: 1261 SILNTCPSHLSELKY 1263
SILNTCPSHLSELKY
Sbjct: 1261 SILNTCPSHLSELKY 1275
BLAST of MC09g0712 vs. ExPASy TrEMBL
Match:
A0A6J1GNB1 (protein TIME FOR COFFEE-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456008 PE=4 SV=1)
HSP 1 Score: 1796 bits (4652), Expect = 0.0
Identity = 1007/1287 (78.24%), Postives = 1078/1287 (83.76%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEK+REARSSSS VAANGSSRRRSRAT FRDLPEEGQVELQETVRLR++G GKR RDR
Sbjct: 1 MIMEKSREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRERG-GKRGRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
EFASRSKR RGG NREEEVEE EGGDTSAED+V DG DSDEVEDGGGVS ILSS TA+SA
Sbjct: 61 EFASRSKRSRGGGNREEEVEE-EGGDTSAEDIVADG-DSDEVEDGGGVSLILSSTTAASA 120
Query: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNPSD 180
SNQN + NSLPPRVAKQQWKVVDDAMIGVPVPRKAR+GNSGVGGAGEDIADD SHRN SD
Sbjct: 121 SNQNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKARSGNSGVGGAGEDIADDHSHRNQSD 180
Query: 181 SPARSSAEVVSPS---IFAKKKMKPTGPKTRTMKASKDSSPST-EGDIEIEIAEVLFGLK 240
SPARSSAEVVSPS + +KKKMK +GPKTR+MK SK SSPS EGDIEIEIAEVLFGLK
Sbjct: 181 SPARSSAEVVSPSTSVVSSKKKMKSSGPKTRSMKTSKGSSPSAMEGDIEIEIAEVLFGLK 240
Query: 241 KQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGAIS 300
KQPQCS+KQE+ PSSNQETE+SS LR+GTK PVTST ANSAQT+FQKSVSLQKN AIS
Sbjct: 241 KQPQCSKKQEVNMNPSSNQETEDSSVLRDGTKPPVTSTAANSAQTAFQKSVSLQKNDAIS 300
Query: 301 DLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIE-------GASDFGMNLGS 360
DLLLNVAVEK +S + GAAK ESE+ A IE GASDF +NLGS
Sbjct: 301 DLLLNVAVEKTES-------------LDGAAKAESEKPAEIEIYPSKLGGASDFSLNLGS 360
Query: 361 HGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPED-SPPSKSIP 420
H +T KPE KE T GDEN SKKTV Q+D+ SC+KGDV PED SPP+KSIP
Sbjct: 361 HERKTEEMKPE-----KEVCTSGDENKESKKTVLVQEDVTSCVKGDVDPEDLSPPTKSIP 420
Query: 421 EAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFKEK 480
EAETQKEEKFKFDLMAPP SPERDGL DMVLD KPLSLG++MGK TS+KVENE
Sbjct: 421 EAETQKEEKFKFDLMAPPASPERDGLADMVLDAKPLSLGIQMGKETSNKVENE------- 480
Query: 481 EKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEPST 540
D +V+SG KF+FFKLDLE+PQLD NS+ MQEQSQKQQ KGAAGTVE N+PST
Sbjct: 481 --------DSIVTSGTKFEFFKLDLEKPQLDRNSIAMQEQSQKQQPKGAAGTVENNDPST 540
Query: 541 SVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLTHY 600
SVRLP+ LGGWPT EIPPVGYMPPFRT+L VD DKSS+KLQHP FILSHPR KRCLTHY
Sbjct: 541 SVRLPVILGGWPTTEIPPVGYMPPFRTVLPVDIVDKSSTKLQHPKFILSHPRPKRCLTHY 600
Query: 601 DIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLNTA 660
DIARNIYLHQQFTKTNYFHP+ DAS SLAEAKLKN+SSKEG+ L NPL + LDMNLN+A
Sbjct: 601 DIARNIYLHQQFTKTNYFHPSGDASASLAEAKLKNMSSKEGMILSNPLGGNHLDMNLNSA 660
Query: 661 ----QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSM-PGSGFIFPISQ 720
QAE DFPGNVVNDK PEAA ADMAKSKQLVFQQ G SFGNSM PGSGFIFPISQ
Sbjct: 661 RQKEQAEVDFPGNVVNDKVPEAAYLADMAKSKQLVFQQGQGASFGNSMVPGSGFIFPISQ 720
Query: 721 HQASIAATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPY 780
HQA AATANQSG+TKSSNQST LF+NPEAGTLVSFPA PAVSSNMSY+HPN+VSIEAPY
Sbjct: 721 HQAPTAATANQSGSTKSSNQSTPLFTNPEAGTLVSFPAFPAVSSNMSYTHPNVVSIEAPY 780
Query: 781 LAKLQNNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQS 840
L K QNN YPFTFSTPAGTS TYRA+SAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQ
Sbjct: 781 LGKFQNNSYPFTFSTPAGTSATYRASSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHHQP 840
Query: 841 SSSHKQPQAQQQQWSVHVPGNNLVPSNAMQVKQ-STEQHLPLSNQSRKHDGETGGEDTAA 900
S SHKQPQ QQQQWSVHVPGNN+ PSN MQVKQ STEQHLPL QSRKH+ ET GED+ +
Sbjct: 841 SGSHKQPQTQQQQWSVHVPGNNVPPSNGMQVKQQSTEQHLPLPKQSRKHENETSGEDSTS 900
Query: 901 LSDKRVAPVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTVAL 960
LSD R A VQK+ YGQNFILPV ALGFTLMPS T NN NGGN+GEKKQSHPQNLK+TVAL
Sbjct: 901 LSDMRPASVQKSAYGQNFILPVPALGFTLMPSGTFNNGNGGNSGEKKQSHPQNLKSTVAL 960
Query: 961 VPSQGLTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQ 1020
VPSQGLTMSFASYNGNGTPSNLNFTP TV+QNLPDMQR GFQ APAPQATKQK+HQ
Sbjct: 961 VPSQGLTMSFASYNGNGTPSNLNFTP-----TVFQNLPDMQRTGFQGAPAPQATKQKNHQ 1020
Query: 1021 SSEGRNGVVLSGSNDRNNGNS-AKPSSTSGQTLVFSNSGRTLNFMTSSAPVNWPSPSTKS 1080
SEGRNGVV+SG++DRN GNS AKPS+TSGQTLVF NS RTLNFMTSS PVNWPSPST
Sbjct: 1021 ISEGRNGVVVSGTSDRNTGNSSAKPSATSGQTLVFGNSSRTLNFMTSSVPVNWPSPST-- 1080
Query: 1081 AALTTNRSAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSLPSSVGPKFPYN 1140
TNR A SSSGNQQQ HILQQQQATM+ R+K P+NNSLPSS KFP +
Sbjct: 1081 ----TNRPAGSSSGNQQQ---------HILQQQQATMVPRSKGPSNNSLPSSATTKFPCS 1140
Query: 1141 APPGFSQPLMHCDDS----AQKNSGRMVASVVPMTSLHLASSPAVSKNSPQQKGS-TPQG 1200
APPGFSQPL+ CD+S AQKNSGRMV +VVPMTSLHLASS AV K+S QQKG+ TP G
Sbjct: 1141 APPGFSQPLIQCDNSIQSPAQKNSGRMVGTVVPMTSLHLASSTAVHKSSTQQKGNNTPHG 1200
Query: 1201 QTQISFGGDFKPAFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQPKQDESSSTGGG 1260
QTQISFGGDFKP FTQMQH+PT HSPSSSGKLRNT SKTS SVAP QPKQDESSSTG G
Sbjct: 1201 QTQISFGGDFKPGFTQMQHLPTGGHSPSSSGKLRNTSSKTSPSVAPVQPKQDESSSTGAG 1231
Query: 1261 QKSSPVCGRNVPSILNTCPSHLSELKY 1263
+KSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 KKSSPVCGRNVPSILNTCPSHLSELKY 1231
BLAST of MC09g0712 vs. ExPASy TrEMBL
Match:
A0A1S3BQP7 (protein TIME FOR COFFEE isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492669 PE=4 SV=1)
HSP 1 Score: 1795 bits (4649), Expect = 0.0
Identity = 1021/1298 (78.66%), Postives = 1087/1298 (83.74%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEK REARSSSS VAANGSSRRRSRAT FRDLPEEGQVELQETVRLRD+G GKR+RDR
Sbjct: 1 MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRG-GKRERDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGG-GVSRILSSAT-AS 120
EF SRSKRRR G NREEE+EE+EGGDTSAED+V DG DSD VEDGG GVSRILSS T AS
Sbjct: 61 EFVSRSKRRREGGNREEEMEEEEGGDTSAEDIVADG-DSDGVEDGGVGVSRILSSTTTAS 120
Query: 121 SASNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRNP 180
S SNQN + NSLPPRV KQQWKV DDAMIGVPVPRKAR+GNSGVGGAGEDIADD SHRN
Sbjct: 121 SVSNQNQKRNSLPPRVVKQQWKVADDAMIGVPVPRKARSGNSGVGGAGEDIADDHSHRNQ 180
Query: 181 SDSPARSSAEVVSPS---IFAKKKMKPTGPKTRTMKASKDSSPST-EGDIEIEIAEVLFG 240
+DSPARSSAEVVSPS I AKKKMKPTGPKTR+MK S SS S EGDIEIEIAEVLFG
Sbjct: 181 TDSPARSSAEVVSPSTSIISAKKKMKPTGPKTRSMKTSNVSSASAKEGDIEIEIAEVLFG 240
Query: 241 LKKQPQ-CSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNG 300
LKKQPQ CS+KQE+ K SS QETENSS LR+G+KS +TST ANSAQT+F KSVSLQKN
Sbjct: 241 LKKQPQHCSKKQEVNMKQSSKQETENSSVLRDGSKSSITSTMANSAQTAFNKSVSLQKND 300
Query: 301 AISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGI-------EGASDFGMN 360
ISDL LNVA EK K + S PL A KGESE+ A I EGAS
Sbjct: 301 VISDLSLNVAGEKQKVD---SLPLDF------AVKGESEKPAEIAIYPSKLEGAS----- 360
Query: 361 LGSHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKS 420
EESK KE FTGGDEN GSK+T Q+D+ SC KGDV PEDSPP+KS
Sbjct: 361 -------------EESKPAKEVFTGGDENKGSKRTGLAQEDITSCAKGDVDPEDSPPNKS 420
Query: 421 IPEAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFK 480
IPEA TQKEEKFKFDLMAPPTSPERDGL DMVLD KPLSLG+EMGK TS KVENEVEG K
Sbjct: 421 IPEAVTQKEEKFKFDLMAPPTSPERDGLADMVLDTKPLSLGIEMGKETSSKVENEVEGLK 480
Query: 481 EKEKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGAAGTVEKNEP 540
EKEKVI+E DK+V+SG KF+FFKLDLE+PQLDSN++TMQEQS KQQ KGAA TVEKNEP
Sbjct: 481 EKEKVINE--DKIVTSGTKFEFFKLDLEKPQLDSNNITMQEQSPKQQPKGAASTVEKNEP 540
Query: 541 STSVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPRSKRCLT 600
STSVRLPI LGGWPT EIP VGYMPPFRT+L VDS DKSS+KLQH NFILSHPR KRCLT
Sbjct: 541 STSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHLNFILSHPRPKRCLT 600
Query: 601 HYDIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHLLDMNLN 660
HYDIARNIYLHQQFTKTNYFHPA DAS SLAEAKLKN+SSKEG+ L NPL + LD+NLN
Sbjct: 601 HYDIARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNISSKEGMLLSNPLSGNHLDVNLN 660
Query: 661 TA----QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFPIS 720
+ Q EGD PGNVVNDKS EAANFAD+AKSKQLVF Q+ G FGNSMP SGFIFPI
Sbjct: 661 SVRQKEQGEGDLPGNVVNDKSSEAANFADIAKSKQLVFHQKQGVPFGNSMPSSGFIFPIG 720
Query: 721 QHQASIA-ATANQSGTTKSSN-QSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIE 780
QHQAS+A ATANQSG+ KSSN QSTSLFSNPE GTLVSFPA PAVS+NMSYSHPN+VS+E
Sbjct: 721 QHQASVAQATANQSGSAKSSNNQSTSLFSNPETGTLVSFPAFPAVSTNMSYSHPNVVSVE 780
Query: 781 APYLAKLQNNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSH 840
APYLAKLQNNGYPFTFSTPAGTS TYR N+AQPLPLFNGSFY SQ+FHPSQIQPAQTQSH
Sbjct: 781 APYLAKLQNNGYPFTFSTPAGTSATYRTNNAQPLPLFNGSFYSSQMFHPSQIQPAQTQSH 840
Query: 841 HQSSSSHKQPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDT 900
HQ S SHKQPQ Q QQWSVHVPGNN++PSN MQVKQSTEQHLPLSNQSRKH+ ET GEDT
Sbjct: 841 HQPSGSHKQPQTQPQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSNQSRKHENETSGEDT 900
Query: 901 AALSDKRVAPVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQSHPQNLKNTV 960
+LSDKR APVQKN YGQN+ILPVQALGFTLMPS T+NN N GN GEKKQSHPQNLKNTV
Sbjct: 901 TSLSDKRAAPVQKNAYGQNYILPVQALGFTLMPSTTLNNGNSGNYGEKKQSHPQNLKNTV 960
Query: 961 ALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKS 1020
ALVPSQGLTMSFASYNGNGTPSNLNF+PI+QN TVYQNLPD+QR GFQVA PQATKQK+
Sbjct: 961 ALVPSQGLTMSFASYNGNGTPSNLNFSPISQNTTVYQNLPDIQRTGFQVATVPQATKQKN 1020
Query: 1021 HQSSEGRNGVVLSGSNDRNNGNSA-KPSS-TSGQTLVFSNSGRTLNFMTSSAPVNWPSPS 1080
HQ+SEGRNGVVLSG++DRN GNS+ KPS+ TSGQTLVF N RTLNFMTSS PVNWPSPS
Sbjct: 1021 HQTSEGRNGVVLSGASDRNTGNSSTKPSAATSGQTLVFGNPSRTLNFMTSSVPVNWPSPS 1080
Query: 1081 TKSAALTTNRSAASSSGNQQQQPLLQLPQQ-HILQQQQATMMSR----TKVPANNSLPSS 1140
TKSAA TTNR A SSSGNQQQQPL QLPQQ HILQQQQA M R TKVP NN+LPSS
Sbjct: 1081 TKSAA-TTNRPAGSSSGNQQQQPLPQLPQQQHILQQQQAAMAPRMAPRTKVPTNNTLPSS 1140
Query: 1141 VGPKFPYNAPPGFSQPLMHCDDS----AQKNSGRMVASVVPMTSLHLASSPA-VSKNSPQ 1200
KFP N PPGFSQPL+ CD+S QKNSGRM ASVVP TSLHL+SS V KNS Q
Sbjct: 1141 ATTKFPCNPPPGFSQPLIQCDNSIQSPVQKNSGRMTASVVPTTSLHLSSSSTPVHKNSIQ 1200
Query: 1201 QKGS-TPQGQTQISFGG--DFKPAFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQP 1260
QKGS TPQGQTQISFGG DFKPA+T MQHIPTS HSPSSSGKLRNT SKT+ SV P Q
Sbjct: 1201 QKGSSTPQGQTQISFGGGGDFKPAYTPMQHIPTSGHSPSSSGKLRNTTSKTNPSVTPAQQ 1260
Query: 1261 KQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSELKY 1263
KQDESSSTG GQKSSPVCGRNVPSILNTCPS LSELKY
Sbjct: 1261 KQDESSSTGAGQKSSPVCGRNVPSILNTCPSQLSELKY 1266
BLAST of MC09g0712 vs. ExPASy TrEMBL
Match:
A0A6J1GN28 (protein TIME FOR COFFEE-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456008 PE=4 SV=1)
HSP 1 Score: 1792 bits (4641), Expect = 0.0
Identity = 1007/1298 (77.58%), Postives = 1078/1298 (83.05%), Query Frame = 0
Query: 1 MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60
MIMEK+REARSSSS VAANGSSRRRSRAT FRDLPEEGQVELQETVRLR++G GKR RDR
Sbjct: 1 MIMEKSREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRERG-GKRGRDR 60
Query: 61 EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120
EFASRSKR RGG NREEEVEE EGGDTSAED+V DG DSDEVEDGGGVS ILSS TA+SA
Sbjct: 61 EFASRSKRSRGGGNREEEVEE-EGGDTSAEDIVADG-DSDEVEDGGGVSLILSSTTAASA 120
Query: 121 SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKART------------GNSGVGGAGED 180
SNQN + NSLPPRVAKQQWKVVDDAMIGVPVPRKAR+ GNSGVGGAGED
Sbjct: 121 SNQNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGED 180
Query: 181 IADDPSHRNPSDSPARSSAEVVSPS---IFAKKKMKPTGPKTRTMKASKDSSPST-EGDI 240
IADD SHRN SDSPARSSAEVVSPS + +KKKMK +GPKTR+MK SK SSPS EGDI
Sbjct: 181 IADDHSHRNQSDSPARSSAEVVSPSTSVVSSKKKMKSSGPKTRSMKTSKGSSPSAMEGDI 240
Query: 241 EIEIAEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQ 300
EIEIAEVLFGLKKQPQCS+KQE+ PSSNQETE+SS LR+GTK PVTST ANSAQT+FQ
Sbjct: 241 EIEIAEVLFGLKKQPQCSKKQEVNMNPSSNQETEDSSVLRDGTKPPVTSTAANSAQTAFQ 300
Query: 301 KSVSLQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIE------ 360
KSVSLQKN AISDLLLNVAVEK +S + GAAK ESE+ A IE
Sbjct: 301 KSVSLQKNDAISDLLLNVAVEKTES-------------LDGAAKAESEKPAEIEIYPSKL 360
Query: 361 -GASDFGMNLGSHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVG 420
GASDF +NLGSH +T KPE KE T GDEN SKKTV Q+D+ SC+KGDV
Sbjct: 361 GGASDFSLNLGSHERKTEEMKPE-----KEVCTSGDENKESKKTVLVQEDVTSCVKGDVD 420
Query: 421 PED-SPPSKSIPEAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSH 480
PED SPP+KSIPEAETQKEEKFKFDLMAPP SPERDGL DMVLD KPLSLG++MGK TS+
Sbjct: 421 PEDLSPPTKSIPEAETQKEEKFKFDLMAPPASPERDGLADMVLDAKPLSLGIQMGKETSN 480
Query: 481 KVENEVEGFKEKEKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKG 540
KVENE D +V+SG KF+FFKLDLE+PQLD NS+ MQEQSQKQQ KG
Sbjct: 481 KVENE---------------DSIVTSGTKFEFFKLDLEKPQLDRNSIAMQEQSQKQQPKG 540
Query: 541 AAGTVEKNEPSTSVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFIL 600
AAGTVE N+PSTSVRLP+ LGGWPT EIPPVGYMPPFRT+L VD DKSS+KLQHP FIL
Sbjct: 541 AAGTVENNDPSTSVRLPVILGGWPTTEIPPVGYMPPFRTVLPVDIVDKSSTKLQHPKFIL 600
Query: 601 SHPRSKRCLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPL 660
SHPR KRCLTHYDIARNIYLHQQFTKTNYFHP+ DAS SLAEAKLKN+SSKEG+ L NPL
Sbjct: 601 SHPRPKRCLTHYDIARNIYLHQQFTKTNYFHPSGDASASLAEAKLKNMSSKEGMILSNPL 660
Query: 661 HRHLLDMNLNTA----QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSM 720
+ LDMNLN+A QAE DFPGNVVNDK PEAA ADMAKSKQLVFQQ G SFGNSM
Sbjct: 661 GGNHLDMNLNSARQKEQAEVDFPGNVVNDKVPEAAYLADMAKSKQLVFQQGQGASFGNSM 720
Query: 721 PGSGFIFPISQHQASIAATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYS 780
PGSGFIFPISQHQA AATANQSG+TKSSNQST LF+NPEAGTLVSFPA PAVSSNMSY+
Sbjct: 721 PGSGFIFPISQHQAPTAATANQSGSTKSSNQSTPLFTNPEAGTLVSFPAFPAVSSNMSYT 780
Query: 781 HPNMVSIEAPYLAKLQNNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQI 840
HPN+VSIEAPYL K QNN YPFTFSTPAGTS TYRA+SAQPLPLFNGSFYPSQLFHPSQI
Sbjct: 781 HPNVVSIEAPYLGKFQNNSYPFTFSTPAGTSATYRASSAQPLPLFNGSFYPSQLFHPSQI 840
Query: 841 QPAQTQSHHQSSSSHKQPQAQQQQWSVHVPGNNLVPSNAMQVKQ-STEQHLPLSNQSRKH 900
QPAQTQSHHQ S SHKQPQ QQQQWSVHVPGNN+ PSN MQVKQ STEQHLPL QSRKH
Sbjct: 841 QPAQTQSHHQPSGSHKQPQTQQQQWSVHVPGNNVPPSNGMQVKQQSTEQHLPLPKQSRKH 900
Query: 901 DGETGGEDTAALSDKRVAPVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQS 960
+ ET GED+ +LSD R A VQK+ YGQNFILPV ALGFTLMPS T NN NGGN+GEKKQS
Sbjct: 901 ENETSGEDSTSLSDMRPASVQKSAYGQNFILPVPALGFTLMPSGTFNNGNGGNSGEKKQS 960
Query: 961 HPQNLKNTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAP 1020
HPQNLK+TVALVPSQGLTMSFASYNGNGTPSNLNFTP TV+QNLPDMQR GFQ AP
Sbjct: 961 HPQNLKSTVALVPSQGLTMSFASYNGNGTPSNLNFTP-----TVFQNLPDMQRTGFQGAP 1020
Query: 1021 APQATKQKSHQSSEGRNGVVLSGSNDRNNGNS-AKPSSTSGQTLVFSNSGRTLNFMTSSA 1080
APQATKQK+HQ SEGRNGVV+SG++DRN GNS AKPS+TSGQTLVF NS RTLNFMTSS
Sbjct: 1021 APQATKQKNHQISEGRNGVVVSGTSDRNTGNSSAKPSATSGQTLVFGNSSRTLNFMTSSV 1080
Query: 1081 PVNWPSPSTKSAALTTNRSAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANNSL 1140
PVNWPSPST TNR A SSSGNQQQ HILQQQQATM+ R+K P+NNSL
Sbjct: 1081 PVNWPSPST------TNRPAGSSSGNQQQ---------HILQQQQATMVPRSKGPSNNSL 1140
Query: 1141 PSSVGPKFPYNAPPGFSQPLMHCDDS----AQKNSGRMVASVVPMTSLHLASSPAVSKNS 1200
PSS KFP +APPGFSQPL+ CD+S AQKNSGRMV +VVPMTSLHLASS AV K+S
Sbjct: 1141 PSSATTKFPCSAPPGFSQPLIQCDNSIQSPAQKNSGRMVGTVVPMTSLHLASSTAVHKSS 1200
Query: 1201 PQQKGS-TPQGQTQISFGGDFKPAFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAPPQP 1260
QQKG+ TP GQTQISFGGDFKP FTQMQH+PT HSPSSSGKLRNT SKTS SVAP QP
Sbjct: 1201 TQQKGNNTPHGQTQISFGGDFKPGFTQMQHLPTGGHSPSSSGKLRNTSSKTSPSVAPVQP 1242
Query: 1261 KQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSELKY 1263
KQDESSSTG G+KSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 KQDESSSTGAGKKSSPVCGRNVPSILNTCPSHLSELKY 1242
BLAST of MC09g0712 vs. TAIR 10
Match:
AT3G22380.1 (time for coffee )
HSP 1 Score: 296.6 bits (758), Expect = 9.5e-80
Identity = 427/1447 (29.51%), Postives = 629/1447 (43.47%), Query Frame = 0
Query: 3 MEKTREARSSSSTVAANGSSRRRSRATVFRDLP-EEGQVELQETVRLRDK-GAGKRDRDR 62
M++ REAR A NG SRRR RA FRD P EEG VEL E RLRD+ G+ K+DRDR
Sbjct: 1 MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDR 60
Query: 63 E-----------------FASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVE 122
E SRSKRRRG D+GGD S+E+ V D +E +
Sbjct: 61 ERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESV---NDDEEYD 120
Query: 123 DGG-----------GVSRILSSATASSA-------------------SNQNLRMNSLPP- 182
DGG S +S+A+ SS+ +N + R N+ PP
Sbjct: 121 DGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPT 180
Query: 183 RVAKQQ------------WKVVDDAMIGVPVPRKAR--------------TGNSGVGGAG 242
+V + WK D+ MIGV VPRKAR T GV +G
Sbjct: 181 KVFRSSPSPAPVSPLVSTWKAADE-MIGVSVPRKARSACTKRPHESWASSTTGGGVFASG 240
Query: 243 EDIADDPSHRNPSD---------SPARSSAEVVSPSIFAKKKMKPTGPKTRTM----KAS 302
E I S +P++ SP+ + S S + +K P+G K + + +S
Sbjct: 241 EQIHRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPPKSSSS 300
Query: 303 KDSSP-STEGDIEIEIAEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVT 362
K SSP + + +IEIEIAEVL+G+ + P S+++ + N TE + + E KS V+
Sbjct: 301 KLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQE-----AAGNDLTEAAKSTVE-VKSRVS 360
Query: 363 STTANSAQTSFQKSVSLQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESE 422
S +N QT Q S++L N + S++ +A ++ K H D+ + +SE
Sbjct: 361 SPISN-PQTLPQSSITLAANSSSSNVSA-IAPKRKKPRHVK----YEDDNSSRVTTIKSE 420
Query: 423 QHAGIEGASDFGMNLGSHGCQTVVTKPEES--KLTKEFFTGGDENPGSKKTVSTQDDLPS 482
A + F L S G + +S LT+E D + LP
Sbjct: 421 AEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESNASLDSEKKENNLSKDETILPK 480
Query: 483 CIKGDVGPEDSPPSKSIPEAETQKEEKFKFDLMAPP---TSPERDG--LTDMVLDIKPLS 542
D +KS + ++EKF+ DLMAPP +S ER G + + + KP
Sbjct: 481 VESSSGFRSDGEGAKS----SSPEKEKFEIDLMAPPPVRSSSERGGEMMECVAAEAKPKV 540
Query: 543 LGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVVSSGKKFDF-----FKLDLERPQLDS 602
+E K + + + ++ R ++V+ + F KLDL++ D
Sbjct: 541 TEVETEAKPLLKEDRSDPAIHDSQ---EKKRPRMVAEAEHHKFERNCELKLDLDKS--DH 600
Query: 603 NSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWPTAEIPPVGYMPPFRTMLSVD 662
L + QK + +K ++ + L +++ GWP +P +GYM P + ++ D
Sbjct: 601 VGLVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWP-GGLPTMGYMAPTQGVVPTD 660
Query: 663 SGDKSSSKLQ-HPNFILSHPRSKRCLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEA 722
+ S++ +Q P+ + + PR KRC TH IARNI HQQFTK N F PAA S +
Sbjct: 661 TSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGSAPMYGT 720
Query: 723 KLKNLSSKEGISL-GNPLHRHLLDMNLNTAQAEGDFPGNVVNDK-SPEAANFADMAKSKQ 782
K NLS L G+ L R N V DK S + ++ A+ Q
Sbjct: 721 KACNLSLMPPTELQGSVLGR----------------SSNPVQDKNSQSTSKSSETAQRNQ 780
Query: 783 LVFQQRPGPSFGNS-MPGSGFIFPISQ--HQASIAATAN----QSGTTKSSNQSTSLFSN 842
L+ QQ P NS + G FIFP+ Q H A+ A A+ SG T S +TS N
Sbjct: 781 LMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGPTATSTSMN 840
Query: 843 PEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPAGTSTTYRANS 902
A A PA + MS+S+P M E YLA LQNNGYPF G YR
Sbjct: 841 GSAS------ATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGAQPAYRGAP 900
Query: 903 AQPLPLFNGSFYPSQLFHPSQIQPAQ----------TQSHHQ----------SSSSHKQP 962
QP+P FNGSFY SQ+ P QP + QSH SS++ K
Sbjct: 901 GQPMPFFNGSFYSSQMIQPPHHQPQKQHQQQLTGQMLQSHAPNNQNGSASTGSSAAQKHL 960
Query: 963 QAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAP 1022
Q QQ + ++ + P++ +Q + Q ++ ET GED+ + +D R
Sbjct: 961 QNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSPSTADSR-GS 1020
Query: 1023 VQKNTYGQNFILPVQALGFTLMPSATMNN---ANGGNNGEKKQSHPQNLKNTVALVPSQG 1082
YGQN+ + +Q LM S + ++GEKK S Q K V S G
Sbjct: 1021 RSNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKK-SQQQVSKAGVESFQSPG 1080
Query: 1083 LTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSS--E 1142
M+FA++NG T LN + IAQN ++ ++P+ R G+Q+ A A ++ ++ +S +
Sbjct: 1081 YAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKMNYGASLED 1140
Query: 1143 GRNGVVLSGSNDRNNGNSAKPS----------STSGQTLVFSNSGRTLNFMTSSAPVNWP 1202
G++G + + N + S GQ++ FSN + L + SA +
Sbjct: 1141 GKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNK-QDLADASVSAVTSGS 1200
Query: 1203 SPSTKSAALTTNRSAASSSG----NQQQQPLLQLPQQHI-------------LQQQQ--- 1250
+ S L + SSG + QQ L Q QQH+ LQ+QQ
Sbjct: 1201 IVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYLQKQQRYA 1260
BLAST of MC09g0712 vs. TAIR 10
Match:
AT3G22380.2 (time for coffee )
HSP 1 Score: 296.6 bits (758), Expect = 9.5e-80
Identity = 427/1447 (29.51%), Postives = 629/1447 (43.47%), Query Frame = 0
Query: 3 MEKTREARSSSSTVAANGSSRRRSRATVFRDLP-EEGQVELQETVRLRDK-GAGKRDRDR 62
M++ REAR A NG SRRR RA FRD P EEG VEL E RLRD+ G+ K+DRDR
Sbjct: 1 MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDR 60
Query: 63 E-----------------FASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVE 122
E SRSKRRRG D+GGD S+E+ V D +E +
Sbjct: 61 ERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESV---NDDEEYD 120
Query: 123 DGG-----------GVSRILSSATASSA-------------------SNQNLRMNSLPP- 182
DGG S +S+A+ SS+ +N + R N+ PP
Sbjct: 121 DGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPT 180
Query: 183 RVAKQQ------------WKVVDDAMIGVPVPRKAR--------------TGNSGVGGAG 242
+V + WK D+ MIGV VPRKAR T GV +G
Sbjct: 181 KVFRSSPSPAPVSPLVSTWKAADE-MIGVSVPRKARSACTKRPHESWASSTTGGGVFASG 240
Query: 243 EDIADDPSHRNPSD---------SPARSSAEVVSPSIFAKKKMKPTGPKTRTM----KAS 302
E I S +P++ SP+ + S S + +K P+G K + + +S
Sbjct: 241 EQIHRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPPKSSSS 300
Query: 303 KDSSP-STEGDIEIEIAEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVT 362
K SSP + + +IEIEIAEVL+G+ + P S+++ + N TE + + E KS V+
Sbjct: 301 KLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQE-----AAGNDLTEAAKSTVE-VKSRVS 360
Query: 363 STTANSAQTSFQKSVSLQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESE 422
S +N QT Q S++L N + S++ +A ++ K H D+ + +SE
Sbjct: 361 SPISN-PQTLPQSSITLAANSSSSNVSA-IAPKRKKPRHVK----YEDDNSSRVTTIKSE 420
Query: 423 QHAGIEGASDFGMNLGSHGCQTVVTKPEES--KLTKEFFTGGDENPGSKKTVSTQDDLPS 482
A + F L S G + +S LT+E D + LP
Sbjct: 421 AEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESNASLDSEKKENNLSKDETILPK 480
Query: 483 CIKGDVGPEDSPPSKSIPEAETQKEEKFKFDLMAPP---TSPERDG--LTDMVLDIKPLS 542
D +KS + ++EKF+ DLMAPP +S ER G + + + KP
Sbjct: 481 VESSSGFRSDGEGAKS----SSPEKEKFEIDLMAPPPVRSSSERGGEMMECVAAEAKPKV 540
Query: 543 LGLEMGKGTSHKVENEVEGFKEKEKVIDEVRDKVVSSGKKFDF-----FKLDLERPQLDS 602
+E K + + + ++ R ++V+ + F KLDL++ D
Sbjct: 541 TEVETEAKPLLKEDRSDPAIHDSQ---EKKRPRMVAEAEHHKFERNCELKLDLDKS--DH 600
Query: 603 NSLTMQEQSQKQQHKGAAGTVEKNEPSTSVRLPITLGGWPTAEIPPVGYMPPFRTMLSVD 662
L + QK + +K ++ + L +++ GWP +P +GYM P + ++ D
Sbjct: 601 VGLVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWP-GGLPTMGYMAPTQGVVPTD 660
Query: 663 SGDKSSSKLQ-HPNFILSHPRSKRCLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEA 722
+ S++ +Q P+ + + PR KRC TH IARNI HQQFTK N F PAA S +
Sbjct: 661 TSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGSAPMYGT 720
Query: 723 KLKNLSSKEGISL-GNPLHRHLLDMNLNTAQAEGDFPGNVVNDK-SPEAANFADMAKSKQ 782
K NLS L G+ L R N V DK S + ++ A+ Q
Sbjct: 721 KACNLSLMPPTELQGSVLGR----------------SSNPVQDKNSQSTSKSSETAQRNQ 780
Query: 783 LVFQQRPGPSFGNS-MPGSGFIFPISQ--HQASIAATAN----QSGTTKSSNQSTSLFSN 842
L+ QQ P NS + G FIFP+ Q H A+ A A+ SG T S +TS N
Sbjct: 781 LMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGPTATSTSMN 840
Query: 843 PEAGTLVSFPALPAVSSNMSYSHPNMVSIEAPYLAKLQNNGYPFTFSTPAGTSTTYRANS 902
A A PA + MS+S+P M E YLA LQNNGYPF G YR
Sbjct: 841 GSAS------ATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGAQPAYRGAP 900
Query: 903 AQPLPLFNGSFYPSQLFHPSQIQPAQ----------TQSHHQ----------SSSSHKQP 962
QP+P FNGSFY SQ+ P QP + QSH SS++ K
Sbjct: 901 GQPMPFFNGSFYSSQMIQPPHHQPQKQHQQQLTGQMLQSHAPNNQNGSASTGSSAAQKHL 960
Query: 963 QAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHDGETGGEDTAALSDKRVAP 1022
Q QQ + ++ + P++ +Q + Q ++ ET GED+ + +D R
Sbjct: 961 QNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSPSTADSR-GS 1020
Query: 1023 VQKNTYGQNFILPVQALGFTLMPSATMNN---ANGGNNGEKKQSHPQNLKNTVALVPSQG 1082
YGQN+ + +Q LM S + ++GEKK S Q K V S G
Sbjct: 1021 RSNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKK-SQQQVSKAGVESFQSPG 1080
Query: 1083 LTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAPAPQATKQKSHQSS--E 1142
M+FA++NG T LN + IAQN ++ ++P+ R G+Q+ A A ++ ++ +S +
Sbjct: 1081 YAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKMNYGASLED 1140
Query: 1143 GRNGVVLSGSNDRNNGNSAKPS----------STSGQTLVFSNSGRTLNFMTSSAPVNWP 1202
G++G + + N + S GQ++ FSN + L + SA +
Sbjct: 1141 GKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNK-QDLADASVSAVTSGS 1200
Query: 1203 SPSTKSAALTTNRSAASSSG----NQQQQPLLQLPQQHI-------------LQQQQ--- 1250
+ S L + SSG + QQ L Q QQH+ LQ+QQ
Sbjct: 1201 IVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYLQKQQRYA 1260
BLAST of MC09g0712 vs. TAIR 10
Match:
AT3G63180.1 (TIC-like )
HSP 1 Score: 227.3 bits (578), Expect = 7.1e-59
Identity = 359/1304 (27.53%), Postives = 515/1304 (39.49%), Query Frame = 0
Query: 3 MEKTREARSSSSTVAA-NGSSRRRSRATVFRDLPEEGQVEL-QETVRLRDKGAGKRDRDR 62
M+K REAR S++ +AA NG ++RR R + E + ++ QE +RD+ GK++RD
Sbjct: 1 MDKPREARRSTTNMAASNGVTKRRQR------ISENSREQMHQEVATVRDR-PGKKERDP 60
Query: 63 EFASRSKRRRG---GANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATA 122
E +RSKRRR E +VEED ++ +D
Sbjct: 61 ESFNRSKRRRSERFAPRNERDVEEDSSDESQGDD-------------------------- 120
Query: 123 SSASNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRN 182
++ +P A +V D MIGVPVPRKAR+ A +
Sbjct: 121 DYHQRKSFSSGRIPRHAAS---LMVADEMIGVPVPRKARS------------ACLKRSHD 180
Query: 183 PSDSPARSSAEVVSPS---IFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEIAEVLFG 242
S S EVVSPS + K++ G K+R K K SS + E D+EIEIAEVL G
Sbjct: 181 CRTSSGSHSCEVVSPSSSTLSVKERKIVNGSKSRIPKPPK-SSGTVEDDLEIEIAEVLSG 240
Query: 243 LKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGA 302
LKKQP S++ ++ENS QKS
Sbjct: 241 LKKQPHSSKR---------GDDSENS-----------------------QKS-------- 300
Query: 303 ISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQT 362
KG + AG+E G C+
Sbjct: 301 ------------------------------SEVKGIKQLPAGVE-------ICGLFTCKA 360
Query: 363 VVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQK 422
K T G+K ++ + D+ E P P+ E K
Sbjct: 361 RFDSANIDKSTN----------GAKTSL--------VVASDIVDEKEPRVSEGPQLENNK 420
Query: 423 EE-----KFKFDLMAPPTSPERDG---LTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFK 482
+E K LM PPT P G L +V D L+ + + V+NE+ +
Sbjct: 421 DESHCISKLDIHLMVPPTLPSSSGRVSLLPLVSDYCKRDSALKSKEKIT--VKNEMNPEE 480
Query: 483 EKEKVIDEVRDKVVSSGKKFDFFKLDLERPQLDS---NSLTMQ--EQSQKQQHKGAAGTV 542
K+K +D K + LD+E P ++ ++L +Q + SQ QQ K A
Sbjct: 481 VKQKKVD-----------KRYWLNLDIEGPNQETDRDSNLRLQNLDWSQPQQAKSA---- 540
Query: 543 EKNEPSTSV-RLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPR 602
P +SV LP+ + WP+ +PP G++ +T VD + SS +Q S PR
Sbjct: 541 ----PHSSVLPLPVAVASWPSG-VPPQGHVALIQTGKPVDESNGSSKLVQGAPISASQPR 600
Query: 603 SKRCLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHL 662
KRC TH+ IARNI LHQ F KTN+ S L L+ + + +P +
Sbjct: 601 PKRCATHFFIARNIQLHQHFLKTNHLPTPNKGSVYLKGGDLRPTAGNPSLHGSSP----I 660
Query: 663 LDMNLNTAQAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFP 722
L +N GD + N K+ E+ +FA ++K +P P+ + +P FI+P
Sbjct: 661 LSLNSQPHVRNGD-NISAPNVKASESGHFASTRQNK-----PQPPPASISVVPAPAFIYP 720
Query: 723 ISQHQASIAATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIE 782
+ H + + S TKS + + L S+N S+ + +
Sbjct: 721 ANHHLQPVMVPSKSSRPTKSPHLAVGL-----------------ASANFSHPSSSASEVS 780
Query: 783 APYLAKLQNNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSH 842
+PYL + NN Y F S ST +Q +P +NGSFY Q+F QP Q QS
Sbjct: 781 SPYLTVIPNNAYSFQLS-----STIRGGTPSQAVPFYNGSFYSPQMFQQPP-QPLQRQSQ 840
Query: 843 HQ--------SSSSHKQPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHD 902
Q SSSSH+QPQ V N++ + + +QH +S +
Sbjct: 841 AQRESKASSCSSSSHRQPQ---------------VSVNSLSSQANVQQHRQMSQKF---- 900
Query: 903 GETGGEDTAALSDKRVAPVQK-NTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQS 962
E G +T D R + QK +GQ PVQ
Sbjct: 901 -EVAGVNT----DSRGSHTQKGGPFGQIMAAPVQ-------------------------- 960
Query: 963 HPQNLKNTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAP 1022
PQN +MSFAS + P+ LNF+ G+ ++
Sbjct: 961 -PQN------------FSMSFASIASSAPPATLNFS----------------SNGYHIST 978
Query: 1023 APQATKQKSHQSSEGRNGVVLSGSN--DRNNGNSAKPSS-TSGQTLVFSNSGRTLNFMTS 1082
P QK+HQSSE + G SN D KP +G TLVF N RTLNF++
Sbjct: 1021 PPGVAHQKNHQSSEAKTGGGSCSSNAEDPKKNLQGKPQGMMNGHTLVFDNPSRTLNFVSG 978
Query: 1083 SAPVNWPSPSTKSAALTTNRSAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANN 1142
+ WP P+ + A+ + S + QH+ Q+QQ + R+K+ +
Sbjct: 1081 T----WPPPA--ATAINGDPSVFT---------------QHLTQRQQQS--GRSKMMMTH 978
Query: 1143 SLPSSVGPKFPYNAPPGFSQPLMHCDDSAQKNSGRMVASVVPMTSLHLASSPAVSKNSPQ 1202
S SV P S L C TSL+L K
Sbjct: 1141 SQADSVSATSSQWKNPATSSSLTSC------------------TSLNL-------KQFQS 978
Query: 1203 QKGSTPQGQTQISFGGDFKPAFTQMQHIPTSSHSPSSSGKL---RNTGSKTSSSVA---- 1262
Q+ GQTQISF P +Q + + SPS +G + SK S+S A
Sbjct: 1201 QQQIRTHGQTQISFAAPTNPQPSQGKQGRSGGSSPSVTGSASHGKPANSKVSNSKALLLS 978
Query: 1263 --PPQPKQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSELKY 1264
P + E+ ++G QK+SPVCGR VP I+ +CP HLSELKY
Sbjct: 1261 PVPLSQEHTENPASGSTQKNSPVCGRTVPPIITSCPGHLSELKY 978
BLAST of MC09g0712 vs. TAIR 10
Match:
AT3G63180.2 (TIC-like )
HSP 1 Score: 221.9 bits (564), Expect = 3.0e-57
Identity = 358/1304 (27.45%), Postives = 514/1304 (39.42%), Query Frame = 0
Query: 3 MEKTREARSSSSTVAA-NGSSRRRSRATVFRDLPEEGQVEL-QETVRLRDKGAGKRDRDR 62
M+K REAR S++ +AA NG ++RR R + E + ++ QE +RD+ GK++RD
Sbjct: 1 MDKPREARRSTTNMAASNGVTKRRQR------ISENSREQMHQEVATVRDR-PGKKERDP 60
Query: 63 EFASRSKRRRG---GANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATA 122
E +RSKRRR E +VEED ++ +D
Sbjct: 61 ESFNRSKRRRSERFAPRNERDVEEDSSDESQGDD-------------------------- 120
Query: 123 SSASNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTGNSGVGGAGEDIADDPSHRN 182
++ +P A +V D MIGVPVPRKAR+ A +
Sbjct: 121 DYHQRKSFSSGRIPRHAAS---LMVADEMIGVPVPRKARS------------ACLKRSHD 180
Query: 183 PSDSPARSSAEVVSPS---IFAKKKMKPTGPKTRTMKASKDSSPSTEGDIEIEIAEVLFG 242
S S EVVSPS + K++ G K+R K K SS + E D+EIEIAEVL G
Sbjct: 181 CRTSSGSHSCEVVSPSSSTLSVKERKIVNGSKSRIPKPPK-SSGTVEDDLEIEIAEVLSG 240
Query: 243 LKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQKSVSLQKNGA 302
LKKQP S++ ++ENS QKS
Sbjct: 241 LKKQPHSSKR---------GDDSENS-----------------------QKS-------- 300
Query: 303 ISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAGIEGASDFGMNLGSHGCQT 362
KG + AG+E G C+
Sbjct: 301 ------------------------------SEVKGIKQLPAGVE-------ICGLFTCKA 360
Query: 363 VVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVGPEDSPPSKSIPEAETQK 422
K T G+K ++ + D+ E P P+ E K
Sbjct: 361 RFDSANIDKSTN----------GAKTSL--------VVASDIVDEKEPRVSEGPQLENNK 420
Query: 423 EE-----KFKFDLMAPPTSPERDG---LTDMVLDIKPLSLGLEMGKGTSHKVENEVEGFK 482
+E K LM PPT P G L +V D L+ + + V+NE+ +
Sbjct: 421 DESHCISKLDIHLMVPPTLPSSSGRVSLLPLVSDYCKRDSALKSKEKIT--VKNEMNPEE 480
Query: 483 EKEKVIDEVRDKVVSSGKKFDFFKLDLERPQLDS---NSLTMQ--EQSQKQQHKGAAGTV 542
K+K +D K + LD+E P ++ ++L +Q + SQ QQ K A
Sbjct: 481 VKQKKVD-----------KRYWLNLDIEGPNQETDRDSNLRLQNLDWSQPQQAKSA---- 540
Query: 543 EKNEPSTSV-RLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILSHPR 602
P +SV LP+ + WP+ +PP G++ +T VD + SS +Q PR
Sbjct: 541 ----PHSSVLPLPVAVASWPSG-VPPQGHVALIQTGKPVDESNGSSKLVQ--------PR 600
Query: 603 SKRCLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLHRHL 662
KRC TH+ IARNI LHQ F KTN+ S L L+ + + +P +
Sbjct: 601 PKRCATHFFIARNIQLHQHFLKTNHLPTPNKGSVYLKGGDLRPTAGNPSLHGSSP----I 660
Query: 663 LDMNLNTAQAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSMPGSGFIFP 722
L +N GD + N K+ E+ +FA ++K +P P+ + +P FI+P
Sbjct: 661 LSLNSQPHVRNGD-NISAPNVKASESGHFASTRQNK-----PQPPPASISVVPAPAFIYP 720
Query: 723 ISQHQASIAATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYSHPNMVSIE 782
+ H + + S TKS + + L S+N S+ + +
Sbjct: 721 ANHHLQPVMVPSKSSRPTKSPHLAVGL-----------------ASANFSHPSSSASEVS 780
Query: 783 APYLAKLQNNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSH 842
+PYL + NN Y F S ST +Q +P +NGSFY Q+F QP Q QS
Sbjct: 781 SPYLTVIPNNAYSFQLS-----STIRGGTPSQAVPFYNGSFYSPQMFQQPP-QPLQRQSQ 840
Query: 843 HQ--------SSSSHKQPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSNQSRKHD 902
Q SSSSH+QPQ V N++ + + +QH +S +
Sbjct: 841 AQRESKASSCSSSSHRQPQ---------------VSVNSLSSQANVQQHRQMSQKF---- 900
Query: 903 GETGGEDTAALSDKRVAPVQK-NTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQS 962
E G +T D R + QK +GQ PVQ
Sbjct: 901 -EVAGVNT----DSRGSHTQKGGPFGQIMAAPVQ-------------------------- 960
Query: 963 HPQNLKNTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAP 1022
PQN +MSFAS + P+ LNF+ G+ ++
Sbjct: 961 -PQN------------FSMSFASIASSAPPATLNFS----------------SNGYHIST 970
Query: 1023 APQATKQKSHQSSEGRNGVVLSGSN--DRNNGNSAKPSS-TSGQTLVFSNSGRTLNFMTS 1082
P QK+HQSSE + G SN D KP +G TLVF N RTLNF++
Sbjct: 1021 PPGVAHQKNHQSSEAKTGGGSCSSNAEDPKKNLQGKPQGMMNGHTLVFDNPSRTLNFVSG 970
Query: 1083 SAPVNWPSPSTKSAALTTNRSAASSSGNQQQQPLLQLPQQHILQQQQATMMSRTKVPANN 1142
+ WP P+ + A+ + S + QH+ Q+QQ + R+K+ +
Sbjct: 1081 T----WPPPA--ATAINGDPSVFT---------------QHLTQRQQQS--GRSKMMMTH 970
Query: 1143 SLPSSVGPKFPYNAPPGFSQPLMHCDDSAQKNSGRMVASVVPMTSLHLASSPAVSKNSPQ 1202
S SV P S L C TSL+L K
Sbjct: 1141 SQADSVSATSSQWKNPATSSSLTSC------------------TSLNL-------KQFQS 970
Query: 1203 QKGSTPQGQTQISFGGDFKPAFTQMQHIPTSSHSPSSSGKL---RNTGSKTSSSVA---- 1262
Q+ GQTQISF P +Q + + SPS +G + SK S+S A
Sbjct: 1201 QQQIRTHGQTQISFAAPTNPQPSQGKQGRSGGSSPSVTGSASHGKPANSKVSNSKALLLS 970
Query: 1263 --PPQPKQDESSSTGGGQKSSPVCGRNVPSILNTCPSHLSELKY 1264
P + E+ ++G QK+SPVCGR VP I+ +CP HLSELKY
Sbjct: 1261 PVPLSQEHTENPASGSTQKNSPVCGRTVPPIITSCPGHLSELKY 970
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q94KE2 | 1.3e-78 | 29.51 | Protein TIME FOR COFFEE OS=Arabidopsis thaliana OX=3702 GN=TIC PE=1 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DAB7 | 0.0 | 100.00 | protein TIME FOR COFFEE isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101848... | [more] |
| A0A6J1DB26 | 0.0 | 99.06 | protein TIME FOR COFFEE isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101848... | [more] |
| A0A6J1GNB1 | 0.0 | 78.24 | protein TIME FOR COFFEE-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114... | [more] |
| A0A1S3BQP7 | 0.0 | 78.66 | protein TIME FOR COFFEE isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492669 PE=4 ... | [more] |
| A0A6J1GN28 | 0.0 | 77.58 | protein TIME FOR COFFEE-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114... | [more] |
