MC09g0386.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0386.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	pre-mRNA-splicing factor ISY1 homolog
Location	MC09: 3518060 .. 3519422 (+)
Sequence length	1363
RNA-Seq Expression	MC09g0386.1
Synteny	MC09g0386.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCGAAATGAGGAGAAAGCACAGTCAATGCTAAATCGTTTCATTGCCTTAAAAGCTGAGGAGAAGAAGAAACCTAAGGAACGCCGACCCTACCTTGCCTCTGAGTGTCGTGACCTTGCAGAAGCTGACAAATGGCGTCAGCAAATCATGCGTGAGATTGGGCGTAAGGTTGCAGAGATCCAAAATGAAGGCCTTGGTGAGCATCGCTTACGTGATCTCAATGATGAGATCAACAAGCTTATCAGAGAAAAGTCACATTGGGAGCGTCGTATAGTTGAGCTAGGTGGCCCCAATTATACCAAACACTCGGCTAAAATGACTGACTTGGACGGAAACATTGTTGATGTACCAAACCCAAGTGGTCGTGGTCCTGGATATCGGTATTTTGGTGCTGCTAAGAAGTTACCAGGTGTGAGGGAGTTGTTTGAGAAGCCACCTGAACTAAGGAAGCGTAGGACTCGTTATGACATATACAAGAGGATCGATGCAAGCTACTATGGCTATAGGGATGACGAGGATGGGGTTTTGGAAAGAGTGGAAGGTCCAAGTGAAGAGCGTATGAGAACCGAGGCAGTCGAAGAGTGGCAGAGAATGGAAGAGATAAGGAAAGAAGCGAAGAGGGCAGTAAAGAGCGGGGAGGTAGCGAGCGTGGTGAACGTTGCAGCAAAGGAGGTACTATTTGAGGAGGAGGAAGAGGTTGTGGAAGAAGAGAGGAAAAGAGAGAGGGAAATGAAGGAGAATTTGGACAAGGAGAGGGAGTTTGTGGTGCATGTGCCTTTACCTGATGAGAAAGAGATTGAGAAGATGGTACTGGAGAAGAAGAAGATGGAACTGTTGAGTAAGTATGCGAGTGATATGCTTTTGGATGAACAGAGCGAAGCCAAGACCATGCTCAATATACAGAGGTAGACTAGATAGATGGTATGCCCCTAGTTTAGTTAGTGCCCAAAATTGTAATAGATTTATTTTTGTTAAATCTGATATGCATCACTGGAAATGCAATTGCCAAATGAATTTGGTTTGTCCATTGATTTCTTGGTTGTGTGGTGGATTGGCCAATCATATTTCAGATCCAACCAGAGGGACAGCAAAGACCGAGTTGTATGATCAAATACTGTTATGATGTTTGTTTAGCTTCTCCCTATATTTTTGCTATGAACATCTTAAAGGTGCAATCAGCGTTGATGAATGATGAAAAACCTCAGATAATAGGTTGCAAGAATTTACCAAATCCCCACTCTCCGAGGGCAAGAATCTCGACAGTGATGTCTCTCTTCCATGTACACAGGACAGTATTTTGGGGCGCATTTGTTTTAAGACTTTGCAGTCGATTTTGGATGCTGACGAATATTTATGGCTT

mRNA sequence

ATGGCTCGAAATGAGGAGAAAGCACAGTCAATGCTAAATCGTTTCATTGCCTTAAAAGCTGAGGAGAAGAAGAAACCTAAGGAACGCCGACCCTACCTTGCCTCTGAGTGTCGTGACCTTGCAGAAGCTGACAAATGGCGTCAGCAAATCATGCGTGAGATTGGGCGTAAGGTTGCAGAGATCCAAAATGAAGGCCTTGGTGAGCATCGCTTACGTGATCTCAATGATGAGATCAACAAGCTTATCAGAGAAAAGTCACATTGGGAGCGTCGTATAGTTGAGCTAGGTGGCCCCAATTATACCAAACACTCGGCTAAAATGACTGACTTGGACGGAAACATTGTTGATGTACCAAACCCAAGTGGTCGTGGTCCTGGATATCGGTATTTTGGTGCTGCTAAGAAGTTACCAGGTGTGAGGGAGTTGTTTGAGAAGCCACCTGAACTAAGGAAGCGTAGGACTCGTTATGACATATACAAGAGGATCGATGCAAGCTACTATGGCTATAGGGATGACGAGGATGGGGTTTTGGAAAGAGTGGAAGGTCCAAGTGAAGAGCGTATGAGAACCGAGGCAGTCGAAGAGTGGCAGAGAATGGAAGAGATAAGGAAAGAAGCGAAGAGGGCAGTAAAGAGCGGGGAGGTAGCGAGCGTGGTGAACGTTGCAGCAAAGGAGGTACTATTTGAGGAGGAGGAAGAGGTTGTGGAAGAAGAGAGGAAAAGAGAGAGGGAAATGAAGGAGAATTTGGACAAGGAGAGGGAGTTTGTGGTGCATGTGCCTTTACCTGATGAGAAAGAGATTGAGAAGATGGTACTGGAGAAGAAGAAGATGGAACTGTTGAGTAAGTATGCGAGTGATATGCTTTTGGATGAACAGAGCGAAGCCAAGACCATGCTCAATATACAGAGGTAGACTAGATAGATGGTATGCCCCTAGTTTAGTTAGTGCCCAAAATTGTAATAGATTTATTTTTGTTAAATCTGATATGCATCACTGGAAATGCAATTGCCAAATGAATTTGGTTTGTCCATTGATTTCTTGGTTGTGTGGTGGATTGGCCAATCATATTTCAGATCCAACCAGAGGGACAGCAAAGACCGAGTTGTATGATCAAATACTGTTATGATGTTTGTTTAGCTTCTCCCTATATTTTTGCTATGAACATCTTAAAGGTGCAATCAGCGTTGATGAATGATGAAAAACCTCAGATAATAGGTTGCAAGAATTTACCAAATCCCCACTCTCCGAGGGCAAGAATCTCGACAGTGATGTCTCTCTTCCATGTACACAGGACAGTATTTTGGGGCGCATTTGTTTTAAGACTTTGCAGTCGATTTTGGATGCTGACGAATATTTATGGCTT

Coding sequence (CDS)

ATGGCTCGAAATGAGGAGAAAGCACAGTCAATGCTAAATCGTTTCATTGCCTTAAAAGCTGAGGAGAAGAAGAAACCTAAGGAACGCCGACCCTACCTTGCCTCTGAGTGTCGTGACCTTGCAGAAGCTGACAAATGGCGTCAGCAAATCATGCGTGAGATTGGGCGTAAGGTTGCAGAGATCCAAAATGAAGGCCTTGGTGAGCATCGCTTACGTGATCTCAATGATGAGATCAACAAGCTTATCAGAGAAAAGTCACATTGGGAGCGTCGTATAGTTGAGCTAGGTGGCCCCAATTATACCAAACACTCGGCTAAAATGACTGACTTGGACGGAAACATTGTTGATGTACCAAACCCAAGTGGTCGTGGTCCTGGATATCGGTATTTTGGTGCTGCTAAGAAGTTACCAGGTGTGAGGGAGTTGTTTGAGAAGCCACCTGAACTAAGGAAGCGTAGGACTCGTTATGACATATACAAGAGGATCGATGCAAGCTACTATGGCTATAGGGATGACGAGGATGGGGTTTTGGAAAGAGTGGAAGGTCCAAGTGAAGAGCGTATGAGAACCGAGGCAGTCGAAGAGTGGCAGAGAATGGAAGAGATAAGGAAAGAAGCGAAGAGGGCAGTAAAGAGCGGGGAGGTAGCGAGCGTGGTGAACGTTGCAGCAAAGGAGGTACTATTTGAGGAGGAGGAAGAGGTTGTGGAAGAAGAGAGGAAAAGAGAGAGGGAAATGAAGGAGAATTTGGACAAGGAGAGGGAGTTTGTGGTGCATGTGCCTTTACCTGATGAGAAAGAGATTGAGAAGATGGTACTGGAGAAGAAGAAGATGGAACTGTTGAGTAAGTATGCGAGTGATATGCTTTTGGATGAACAGAGCGAAGCCAAGACCATGCTCAATATACAGAGGTAG

Protein sequence

MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLNIQR

Homology

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BLAST of MC09g0386.1 vs. ExPASy Swiss-Prot
Match: Q69ZQ2 (Pre-mRNA-splicing factor ISY1 homolog OS=Mus musculus OX=10090 GN=Isy1 PE=1 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 6.5e-64
Identity = 150/299 (50.17%), Postives = 197/299 (65.89%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+
Sbjct: 1   MARNAEKAMTALARFRQAQLEE-GKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQ 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y K   KM D +G  V    P
Sbjct: 61  IQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEV----P 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
             R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +
Sbjct: 121 GNR--GYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPL 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           E   E+++R E VE+W+   E R       +  E    +N+ A  V  EE +E   +E+ 
Sbjct: 181 EQEYEKKLRAELVEKWKAEREARLARGEKEEEEEEEEEINIYA--VTEEESDEEGNQEKA 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTML 300
            E       D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  EAK +L
Sbjct: 241 GE-------DGQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEAKRLL 283

[Close]

BLAST of MC09g0386.1 vs. ExPASy Swiss-Prot
Match: Q6AYB3 (Pre-mRNA-splicing factor ISY1 homolog OS=Rattus norvegicus OX=10116 GN=Isy1 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 6.5e-64
Identity = 152/299 (50.84%), Postives = 199/299 (66.56%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+
Sbjct: 1   MARNAEKAMTALARFRQAQLEE-GKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQ 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y K   KM D +G  V    P
Sbjct: 61  IQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEV----P 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
             R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +
Sbjct: 121 GNR--GYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPL 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           E   E+R+R E VE+W+   E R  A+   +  E    +N+ A  V  EE +E   +E+ 
Sbjct: 181 EQEYEKRLRAELVEKWKAEREARL-ARGEKEEEEEEEEINIYA--VTEEESDEEGSQEKA 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTML 300
            E       D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  EAK +L
Sbjct: 241 GE-------DGQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEAKRLL 282

[Close]

BLAST of MC09g0386.1 vs. ExPASy Swiss-Prot
Match: Q9ULR0 (Pre-mRNA-splicing factor ISY1 homolog OS=Homo sapiens OX=9606 GN=ISY1 PE=1 SV=3)

HSP 1 Score: 243.4 bits (620), Expect = 3.2e-63
Identity = 155/305 (50.82%), Postives = 199/305 (65.25%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+
Sbjct: 1   MARNAEKAMTALARFRQAQLEE-GKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQ 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y K   KM D +G  V    P
Sbjct: 61  IQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEV----P 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
             R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +
Sbjct: 121 GNR--GYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPL 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEE-----VV 240
           E   E+++R E VE+W+   E R      +  GE         KE   EEEEE     V 
Sbjct: 181 EQEYEKKLRAELVEKWKAEREAR------LARGE---------KEEEEEEEEEINIYAVT 240

Query: 241 EEERKRE-REMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSE 300
           EEE   E  + K   D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  E
Sbjct: 241 EEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEE 283

[Close]

BLAST of MC09g0386.1 vs. ExPASy Swiss-Prot
Match: Q54N41 (Pre-mRNA-splicing factor ISY1 homolog OS=Dictyostelium discoideum OX=44689 GN=isy1 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-54
Identity = 133/306 (43.46%), Postives = 187/306 (61.11%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKA+SMLNR++ LK  E K+ +ERRPYL++EC  L +A+KWR+QI++EI R + E
Sbjct: 1   MARNEEKAKSMLNRYLQLKGTESKQ-EERRPYLSNECDSLVDAEKWRRQILKEITRGITE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN  L E+++RDLND INKL+REK HWERRI++LGGPN+   + K+ D DG       P
Sbjct: 61  IQNSALDEYKIRDLNDHINKLVREKGHWERRILQLGGPNHRALAPKLFDADGK-----EP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPEL---RKRRTRYDIYKRIDASYYGYRDDEDGVL 180
            G G  YRY+G AK LPGV ELFEKP +     +++TR D+YK ID+ YYGYRDDEDG L
Sbjct: 121 LGTGT-YRYYGEAKNLPGVAELFEKPEQTLHGTEKKTRQDLYKYIDSDYYGYRDDEDGKL 180

Query: 181 ERVEGPSEERMRTEAVEEWQRMEEIRKEA------KRAVKSGEVASVVNVAAKEVLFEEE 240
           E +E   E+    + VE W++ +  + +          +K+    +       E+  +++
Sbjct: 181 EEIEREYEKYAIEQNVENWKKQQRDKFQINTNRFNNNNIKNSIAINKNTENKDEINIDDD 240

Query: 241 EEVVEEERKRER------EMKENLDKERE-----FVVHVPLPDEKEIEKMVLEKKKMELL 287
           EE  +             E  EN+ +E E     F  +V LP   +IE ++LEK+K EL 
Sbjct: 241 EENDDNNNNNNNNNTENTENIENIQEEEEKEDIKFKSYVSLPSTSQIETILLEKRKEELR 299

[Close]

BLAST of MC09g0386.1 vs. ExPASy Swiss-Prot
Match: Q20716 (Protein isy-1 OS=Caenorhabditis elegans OX=6239 GN=isy-1 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.0e-45
Identity = 121/284 (42.61%), Postives = 177/284 (62.32%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARN EKA + L R+  +K EE++ P  RRP+   +CR+L++A+++R++I+R+  +K+  
Sbjct: 1   MARNAEKAMTALARWRRMKEEEERGPIARRPHDVKDCRNLSDAERFRREIVRDASKKITA 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN GLGE +LRDLNDE+N+LI+ K  WE+RI ELGG +Y K++ K  D  G        
Sbjct: 61  IQNPGLGEFKLRDLNDEVNRLIKLKHAWEQRIRELGGTDYRKYAQKELDAIG------RE 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPE-LRKRRTRYDIYKRIDASYYGYRDDEDGVLER 180
           +G   GY+YFGAAK LPGVRELFEK  E   +RR R D+ + IDA Y+GY DDEDG L  
Sbjct: 121 TGNSRGYKYFGAAKDLPGVRELFEKSTEGEEQRRHRADLLRNIDAHYFGYLDDEDGRLIP 180

Query: 181 VEGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEER 240
           +E   EE+           +E I KE   A K  +     + AA E +++ EE+  +++ 
Sbjct: 181 LEKLIEEK----------NIERINKEF--AEKQAQKQQTASDAAPENIYKVEED-DDDDL 240

Query: 241 KREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKY 284
           + +       D     + HV LP +++IE+M+LE+KK EL++KY
Sbjct: 241 ETQESTVIGEDGRPMTIRHVLLPTQQDIEEMLLEQKKQELMAKY 265

[Close]

BLAST of MC09g0386.1 vs. NCBI nr
Match: XP_022152151.1 (pre-mRNA-splicing factor ISY1 homolog [Momordica charantia])

HSP 1 Score: 567 bits (1462), Expect = 2.47e-203
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. NCBI nr
Match: XP_011659495.1 (pre-mRNA-splicing factor ISY1 homolog [Cucumis sativus] >KGN45211.1 hypothetical protein Csa_016162 [Cucumis sativus])

HSP 1 Score: 562 bits (1448), Expect = 3.37e-201
Identity = 299/303 (98.68%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTKHSAKMTDLDGNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMR EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. NCBI nr
Match: XP_008461190.1 (PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Cucumis melo] >XP_038906088.1 pre-mRNA-splicing factor ISY1 homolog [Benincasa hispida] >XP_038906089.1 pre-mRNA-splicing factor ISY1 homolog [Benincasa hispida] >KAA0058702.1 pre-mRNA-splicing factor ISY1-like protein [Cucumis melo var. makuwa] >TYK10508.1 pre-mRNA-splicing factor ISY1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 560 bits (1444), Expect = 1.37e-200
Identity = 298/303 (98.35%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTKHSAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMR EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. NCBI nr
Match: XP_023004198.1 (pre-mRNA-splicing factor ISY1 homolog [Cucurbita maxima] >XP_023514817.1 pre-mRNA-splicing factor ISY1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557 bits (1436), Expect = 2.27e-199
Identity = 297/303 (98.02%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTK+SAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKYSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEER+R EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEER+
Sbjct: 181 EGPSEERIRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERR 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAKTMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. NCBI nr
Match: XP_022960451.1 (pre-mRNA-splicing factor ISY1 homolog [Cucurbita moschata] >KAG6593361.1 Pre-mRNA-splicing factor ISY1-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025707.1 Pre-mRNA-splicing factor ISY1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 556 bits (1432), Expect = 9.24e-199
Identity = 296/303 (97.69%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNY+K+SAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYSKYSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEER+R EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEER+
Sbjct: 181 EGPSEERIRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERR 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAKTMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. ExPASy TrEMBL
Match: A0A6J1DGR8 (pre-mRNA-splicing factor ISY1 homolog OS=Momordica charantia OX=3673 GN=LOC111019937 PE=3 SV=1)

HSP 1 Score: 567 bits (1462), Expect = 1.20e-203
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. ExPASy TrEMBL
Match: A0A0A0KBN6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G431430 PE=3 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 1.63e-201
Identity = 299/303 (98.68%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTKHSAKMTDLDGNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMR EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. ExPASy TrEMBL
Match: A0A5D3CH05 (Pre-mRNA-splicing factor ISY1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001580 PE=3 SV=1)

HSP 1 Score: 560 bits (1444), Expect = 6.64e-201
Identity = 298/303 (98.35%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTKHSAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMR EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. ExPASy TrEMBL
Match: A0A1S3CE48 (pre-mRNA-splicing factor ISY1 homolog OS=Cucumis melo OX=3656 GN=LOC103499848 PE=3 SV=1)

HSP 1 Score: 560 bits (1444), Expect = 6.64e-201
Identity = 298/303 (98.35%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTKHSAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMR EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. ExPASy TrEMBL
Match: A0A6J1KYR8 (pre-mRNA-splicing factor ISY1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111497599 PE=3 SV=1)

HSP 1 Score: 557 bits (1436), Expect = 1.10e-199
Identity = 297/303 (98.02%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTK+SAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKYSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEER+R EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEER+
Sbjct: 181 EGPSEERIRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERR 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAKTMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]

BLAST of MC09g0386.1 vs. TAIR 10
Match: AT3G18790.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Isy1-like splicing (InterPro:IPR009360); Has 1147 Blast hits to 965 proteins in 236 species: Archae - 12; Bacteria - 13; Metazoa - 351; Fungi - 230; Plants - 49; Viruses - 9; Other Eukaryotes - 483 (source: NCBI BLink). )

HSP 1 Score: 458.8 bits (1179), Expect = 3.5e-129
Identity = 242/303 (79.87%), Postives = 270/303 (89.11%), Query Frame = 0


Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFI  K  EKKKPKERRPYLASECRDLAEADKWRQQI+REIG KVAE
Sbjct: 1   MARNEEKAQSMLNRFITQKESEKKKPKERRPYLASECRDLAEADKWRQQILREIGSKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKL+RE+ HWERRIVELGG NY+KHSAKMTDL+GNI+DVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLLRERYHWERRIVELGGHNYSKHSAKMTDLEGNIIDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKR+TRYDIYKRIDASYYGYRDDEDG+LE++
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRKTRYDIYKRIDASYYGYRDDEDGILEKL 180

Query: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           E  SE  MR  +VEEW+R++E+RKEA++             AA+EVLFEEEE+VVEEER 
Sbjct: 181 ERKSEGGMRKRSVEEWRRLDEVRKEARKGASEVVSVGAAAAAAREVLFEEEEDVVEEER- 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300
            ERE +E  +KEREFVVHVPLPDEKEIEKMVLEKKKM+LLSKYAS+ L+++Q+EAK+MLN
Sbjct: 241 MEREKEE--EKEREFVVHVPLPDEKEIEKMVLEKKKMDLLSKYASEDLVEQQTEAKSMLN 300

Query: 301 IQR 304
           I R
Sbjct: 301 IHR 300

The following BLAST results are available for this feature:

BLAST of MC09g0386.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69ZQ2	6.5e-64	50.17	Pre-mRNA-splicing factor ISY1 homolog OS=Mus musculus OX=10090 GN=Isy1 PE=1 SV=2	[more]
Q6AYB3	6.5e-64	50.84	Pre-mRNA-splicing factor ISY1 homolog OS=Rattus norvegicus OX=10116 GN=Isy1 PE=2...	[more]
Q9ULR0	3.2e-63	50.82	Pre-mRNA-splicing factor ISY1 homolog OS=Homo sapiens OX=9606 GN=ISY1 PE=1 SV=3	[more]
Q54N41	3.6e-54	43.46	Pre-mRNA-splicing factor ISY1 homolog OS=Dictyostelium discoideum OX=44689 GN=is...	[more]
Q20716	3.0e-45	42.61	Protein isy-1 OS=Caenorhabditis elegans OX=6239 GN=isy-1 PE=1 SV=1	[more]

BLAST of MC09g0386.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152151.1	2.47e-203	100.00	pre-mRNA-splicing factor ISY1 homolog [Momordica charantia]	[more]
XP_011659495.1	3.37e-201	98.68	pre-mRNA-splicing factor ISY1 homolog [Cucumis sativus] >KGN45211.1 hypothetical...	[more]
XP_008461190.1	1.37e-200	98.35	PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Cucumis melo] >XP_038906088.1 ...	[more]
XP_023004198.1	2.27e-199	98.02	pre-mRNA-splicing factor ISY1 homolog [Cucurbita maxima] >XP_023514817.1 pre-mRN...	[more]
XP_022960451.1	9.24e-199	97.69	pre-mRNA-splicing factor ISY1 homolog [Cucurbita moschata] >KAG6593361.1 Pre-mRN...	[more]

BLAST of MC09g0386.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGR8	1.20e-203	100.00	pre-mRNA-splicing factor ISY1 homolog OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A0A0KBN6	1.63e-201	98.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G431430 PE=3 SV=1	[more]
A0A5D3CH05	6.64e-201	98.35	Pre-mRNA-splicing factor ISY1-like protein OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3CE48	6.64e-201	98.35	pre-mRNA-splicing factor ISY1 homolog OS=Cucumis melo OX=3656 GN=LOC103499848 PE...	[more]
A0A6J1KYR8	1.10e-199	98.02	pre-mRNA-splicing factor ISY1 homolog OS=Cucurbita maxima OX=3661 GN=LOC11149759...	[more]

BLAST of MC09g0386.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18790.1	3.5e-129	79.87	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 71..91
None	No IPR available	COILS	Coil	Coil	coord: 227..254
IPR009360	Pre-mRNA-splicing factor Isy1	PFAM	PF06246	Isy1	coord: 1..282 e-value: 4.7E-90 score: 301.7
IPR009360	Pre-mRNA-splicing factor Isy1	PANTHER	PTHR13021	PRE-MRNA-SPLICING FACTOR ISY1	coord: 1..300
IPR029012	Helix hairpin bin domain superfamily	GENE3D	1.10.287.660	Helix hairpin bin	coord: 39..96 e-value: 8.3E-30 score: 104.4
IPR037200	Pre-mRNA-splicing factor Isy1 superfamily	SUPERFAMILY	140102	ISY1 domain-like	coord: 27..102

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC09g0386	MC09g0386	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC09g0386.1-cds	MC09g0386.1-cds-MC09:3518060..3518971	CDS

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC09g0386.1-utr3	MC09g0386.1-utr3-MC09:3518972..3519422	utr3

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC09g0386.1	MC09g0386.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000350	generation of catalytic spliceosome for second transesterification step
biological_process	GO:0000389	mRNA 3'-splice site recognition
cellular_component	GO:0071013	catalytic step 2 spliceosome
cellular_component	GO:0071014	post-mRNA release spliceosomal complex
cellular_component	GO:0071020	post-spliceosomal complex
cellular_component	GO:0000974	Prp19 complex