MC08g1774 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1774 vs. ExPASy Swiss-Prot
Match: Q8RY22 (Protease Do-like 7 OS=Arabidopsis thaliana OX=3702 GN=DEGP7 PE=2 SV=1)

HSP 1 Score: 1690.2 bits (4376), Expect = 0.0e+00
Identity = 849/1149 (73.89%), Postives = 956/1149 (83.20%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            M DP E LGS+ A++  +S  K++LC+EIDPPL +++ATA+DWR+AL KVVPAVVVLRTT
Sbjct: 1    MGDPLERLGSQ-ASMATESVMKEDLCLEIDPPLTESVATAEDWRRALGKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGF+VDKRRGIILTNRHVVKPGPVVAEAMFVNREE+P+ P+YRDP
Sbjct: 61   ACRAFDTESAGASYATGFIVDKRRGIILTNRHVVKPGPVVAEAMFVNREEIPIYPVYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFF YDP A+QFL Y+EIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDR+A
Sbjct: 121  VHDFGFFCYDPSAVQFLTYQEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDRDA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPL+RV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLQRV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRAL+FLQK  DS  +K +AV IPRGTLQ TFLHKGFDEIRRLGLRSETEQ+VR ASP G
Sbjct: 241  VRALSFLQKSIDSRTDKPKAVHIPRGTLQMTFLHKGFDEIRRLGLRSETEQVVRHASPTG 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVP GPA K L+PGDVLVR+NG V+TQFL +E L+DD V Q +++++ERGG
Sbjct: 301  ETGMLVVDSVVPSGPADKHLEPGDVLVRVNGTVLTQFLNLENLLDDGVGQILELEIERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
               +V + VQDLHSITPD+FLEVSGAVIHPLSYQQARNFRF C L YV +PGYMLFRAGV
Sbjct: 361  QPLSVSVSVQDLHSITPDHFLEVSGAVIHPLSYQQARNFRFPCGLAYVADPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKK A E+IS + DLVSVLSKLSRG RVPLEY+S+TDRHR+KSVLVT+DHHEWYA
Sbjct: 421  PRHAIIKKVANEDISSLGDLVSVLSKLSRGARVPLEYMSHTDRHRKKSVLVTIDHHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRH----VH 540
            PPQ+Y R+DS+GLW AKPA +P     +S   ++G    N     S D S   H    +H
Sbjct: 481  PPQLYTRNDSSGLWDAKPAIEP-----ASVSPSIG----NNGFPISQDISLCHHDTEPMH 540

Query: 541  QVNNQEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQE 600
            +VN + + D    MET     S    SQ+      KK+RV++D  +DG  A+ S + + E
Sbjct: 541  EVNVRGVTDIAAIMET-----SSGDGSQNDFGSEAKKQRVDEDS-SDGIAANGSLYGS-E 600

Query: 601  PILEDETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQH 660
               +D     T +LRD++G T  A++ANAS  ER IEP LVMFEVHVPPSC LDGVHSQH
Sbjct: 601  FKSDDAMETDTTVLRDFEGAT--ALSANASLAERAIEPALVMFEVHVPPSCSLDGVHSQH 660

Query: 661  FFGTGVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAY 720
            FFGTG+IIYHS +MGL  VDKNTVAISA D+MLSFAAFP+EIPGEVVFLHPVHNYAL+AY
Sbjct: 661  FFGTGIIIYHSSNMGLAVVDKNTVAISASDVMLSFAAFPVEIPGEVVFLHPVHNYALIAY 720

Query: 721  DPSSLGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQR 780
            +PS++    ASV++AA+LLP                                  EPALQR
Sbjct: 721  NPSAMDPASASVIRAAELLP----------------------------------EPALQR 780

Query: 781  GDSVYLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGV 840
            GDSVYLVGLSR+LQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDTDFGS+FSG 
Sbjct: 781  GDSVYLVGLSRNLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDTDFGSSFSGA 840

Query: 841  LTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLING 900
            LTDE GR++AIWGSFSTQ+K+  +SSEDHQFVRGIP+Y ISQVL+KII+G NGP LLING
Sbjct: 841  LTDEQGRIRAIWGSFSTQVKYSSTSSEDHQFVRGIPVYAISQVLEKIITGGNGPALLING 900

Query: 901  VKRPMPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAEN 960
            VKRPMP+VRILEVELYPTLLSKARSFGLSDEW+Q LVKKDP+RRQVLRVKGCLAGSKAEN
Sbjct: 901  VKRPMPLVRILEVELYPTLLSKARSFGLSDEWIQVLVKKDPVRRQVLRVKGCLAGSKAEN 960

Query: 961  LLEQGDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRD 1020
            LLEQGDMVLA+NK P+TCF DIE ACQ LDK   +D  LN+TI RQG E++L+VGTD RD
Sbjct: 961  LLEQGDMVLAVNKMPVTCFNDIEAACQTLDKGSYSDENLNLTILRQGQELELVVGTDKRD 1020

Query: 1021 GNGTTRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEV 1080
            GNGTTRVINWCGC+VQDPHPAVRALGFLPEEGHGVYV RWCHGSP HRYGLYALQWIVEV
Sbjct: 1021 GNGTTRVINWCGCVVQDPHPAVRALGFLPEEGHGVYVTRWCHGSPAHRYGLYALQWIVEV 1080

Query: 1081 NGKLTPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAM 1140
            NGK TPDL+AF + TKELEH QFVR+RTVHLNGKPRVLTLKQ+LHYWPTWELRFDPETA+
Sbjct: 1081 NGKKTPDLNAFADATKELEHGQFVRIRTVHLNGKPRVLTLKQDLHYWPTWELRFDPETAL 1096

Query: 1141 WRRVTIKAL 1146
            WRR  +KAL
Sbjct: 1141 WRRNILKAL 1096

BLAST of MC08g1774 vs. ExPASy Swiss-Prot
Match: Q0UY70 (Pro-apoptotic serine protease NMA111 OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) OX=321614 GN=NMA111 PE=3 SV=2)

HSP 1 Score: 547.4 bits (1409), Expect = 4.0e-154
Identity = 360/1105 (32.58%), Postives = 550/1105 (49.77%), Query Frame = 0

Query: 42   DWRKALNKVVPAVVVLRTTACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVA 101
            +W+K +  VV +VV +      +FDT+ A +S ATGFVVD  +G ILTNRHVV  GP + 
Sbjct: 76   EWQKTIEGVVKSVVSIHFCQTCSFDTDPAISSEATGFVVDAEKGYILTNRHVVGAGPFIG 135

Query: 102  EAMFVNREEVPVRPIYRDPVHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGND 161
              +F N EE  V P+YRDPVHDFG  R+DP AI++++   + L P+ A VG+EIRVVGND
Sbjct: 136  YCIFDNHEECDVYPVYRDPVHDFGILRFDPKAIKYMSVSALQLRPDFAKVGVEIRVVGND 195

Query: 162  SGEKVSILAGTLARLDREAPHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVAL 221
            +GEK+SIL+G ++RLDR AP Y  +GY+DFNT Y+QAA+   GGSSGSPV++  G AVAL
Sbjct: 196  AGEKLSILSGVISRLDRNAPEY-GEGYSDFNTNYIQAAAAASGGSSGSPVVNRDGFAVAL 255

Query: 222  NAGSKSSSASA-FFLPLERVVRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEI 281
             AG ++  A+  +FLPL+R +RAL  +++G           ++ RGT+Q  ++ K FDE 
Sbjct: 256  QAGGRADGAATDYFLPLDRPLRALELVRRGE----------AVTRGTVQTQWILKPFDEC 315

Query: 282  RRLGLRSETEQMVRLASPPGETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKM 341
            RRLGL ++ E+ VR   P  ETGMLV + V+P GPA   ++ GD+L+++NGE ITQF+++
Sbjct: 316  RRLGLSTDLEKAVRTQFPK-ETGMLVAEVVLPQGPASTKVEEGDILIKVNGEFITQFVRL 375

Query: 342  ETLIDDSVNQTIDIQVERGGTSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNF- 401
            ++++DD+V +TI + ++R G +  V L V +LH ITPD F+ VSGA  H LSYQQAR + 
Sbjct: 376  DSILDDNVGKTISVTIQRAGENLEVELDVGNLHDITPDRFVSVSGASFHDLSYQQARLYA 435

Query: 402  -RFKCSLVYVTE-PGYMLFRAGVPRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEY 461
               K + V+V E  G   F  G     +I++   +    ++  + V+ K+    R+ ++Y
Sbjct: 436  ISLKNAGVFVCEAAGSFRFADGYASGWLIQEVDNQPTPNLDTFIEVMKKIPDRKRIVIQY 495

Query: 462  ISYTDRHRRKSVLVTVDHHEWYAPPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDG 521
                D H   + +  VD H W+A  +I  R+D TGLW  KP   P               
Sbjct: 496  KHLRDLHTANTSITAVDRH-WHAKIRIATRNDKTGLWDFKPIADP--------------- 555

Query: 522  YMNQTDVFSDDSSHIRHVHQVNNQEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVE 581
                          +  V QV+ +      V M +N+ +  +  RS              
Sbjct: 556  --------------VPAVPQVSRRA---NFVKMSSNYPNAVDIVRS-------------- 615

Query: 582  DDRLTDGNVADCSFHETQEPILEDETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLV 641
                                                                        
Sbjct: 616  ------------------------------------------------------------ 675

Query: 642  MFEVHVPPSCMLDGVHSQHFFGTGVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIE 701
               VHV     LDG    +  G G+++   Q  GLV V +  +    CDI L   A  I 
Sbjct: 676  FVRVHVSMPIKLDGFPKMNKQGYGLVVDAEQ--GLVLVSRAILPYDLCDISL-IIADSIF 735

Query: 702  IPGEVVFLHPVHNYALVAYDPSSLGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYL 761
            I  +VVF+HP+ NY +V YDP+                                      
Sbjct: 736  IDAKVVFMHPLQNYVIVKYDPA-------------------------------------- 795

Query: 762  TLLHKVSLYPSLTEPALQRGDSVYLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRA 821
             L++     P      +++GD     GL+++ +    K++VT+       +SA +PRYRA
Sbjct: 796  -LVNAPVKTPKFATEFIRKGDETIFFGLNQNFRPVVAKTVVTDITTVAIPASAITPRYRA 855

Query: 822  TNMEVIELDTDFGS-TFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTI 881
            TN + I +DT+  S + SGVL  E G VQA+W S+  +      S +D ++  G+    +
Sbjct: 856  TNFDAITVDTNQASHSGSGVLIAEDGTVQALWLSYLGERT--SHSGKDVEYHLGLATPNL 915

Query: 882  SQVLDKIISGSNGPPLLINGVKRPMPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKD 941
              +L++I SG               P +RIL VE     +S+AR  G+S++W++   + D
Sbjct: 916  LPILNEIKSGKT-------------PKLRILNVEFQTVQMSQARVMGVSEDWIEKTEQAD 975

Query: 942  PIRRQVLRVKGCLAGSKAENLLEQGDMVLAINKQPITCFYDIENACQELDKNYTTDGKLN 1001
            P R Q+  V+   +G   + +LE GD++L +N + +T          +LD  Y  +  L 
Sbjct: 976  PERHQLFMVRKVDSGHGGDGMLE-GDILLTLNGKLVT-------RSPDLDVMYNNE-FLE 993

Query: 1002 MTIFRQGHEIDLLVGTDVRDGNGTTRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARW 1061
              I R+  E  + V T   +   T R++++CG  +  PH AVR    + +    VY++  
Sbjct: 1036 AVIVRKREEKTIKVTTVATEEIETDRMVSFCGATLHRPHQAVRQQ--ISKIHSDVYISSR 993

Query: 1062 CHGSPVHRYGLYALQWIVEVNGKLTPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTL 1121
              GSP + YGL    ++  VN   TPDL  F+   K++   ++ R++ +  +  P V T+
Sbjct: 1096 ARGSPAYMYGLAPTNFLTHVNNIPTPDLSTFLREVKKIGDNEYFRLKVMTFDNVPWVATM 993

Query: 1122 KQNLHYWPTWE-LRFDPETAMWRRV 1141
            K+N HY+PT E ++ D E   W+R+
Sbjct: 1156 KKNEHYFPTIEYVKDDTEALGWKRI 993

BLAST of MC08g1774 vs. ExPASy Swiss-Prot
Match: Q7S9D2 (Pro-apoptotic serine protease nma111 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=nma111 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.4e-153
Identity = 374/1118 (33.45%), Postives = 539/1118 (48.21%), Query Frame = 0

Query: 32   PLRDNLATADDWRKALNKVVPAVVVLRTTACRAFDTESAGASYATGFVVDKRRGIILTNR 91
            PL     TA +W+  +  VV  VV +R     +FDT+ A  S ATGFVVD  RG ILTNR
Sbjct: 61   PLAPGADTA-EWQATIENVVRNVVSIRFCQTCSFDTDPALTSEATGFVVDAERGYILTNR 120

Query: 92   HVVKPGPVVAEAMFVNREEVPVRPIYRDPVHDFGFFRYDPGAIQFLNYEEIPLAPEAASV 151
            HVV  GP     +F N EEV   P+YRDPVHDFG  ++DP AI+++    +PL P+ A V
Sbjct: 121  HVVGSGPFWGYCIFDNHEEVDAYPVYRDPVHDFGILKFDPKAIKYMPVAALPLRPDLARV 180

Query: 152  GLEIRVVGNDSGEKVSILAGTLARLDREAPHYKKDGYNDFNTFYMQAASGTKGGSSGSPV 211
            G+EIRVVGND+GEK+SIL+G ++RLDR AP Y  DGY+DFNT Y QA++   GGSSGSPV
Sbjct: 181  GIEIRVVGNDAGEKLSILSGVISRLDRNAPEY-GDGYSDFNTCYYQASAAASGGSSGSPV 240

Query: 212  IDWQGRAVALNAGSKSSSASA-FFLPLERVVRALTFLQKGRDSYVNKWEAVSIPRGTLQA 271
            ++  G AVAL AG ++  AS  +FLPL+R +RAL  LQ+G+           I RG +Q 
Sbjct: 241  VNKDGFAVALQAGGRADGASTDYFLPLDRPLRALKCLQEGK----------PITRGDIQC 300

Query: 272  TFLHKGFDEIRRLGLRSETEQMVRLASPPGETGMLVVDSVVPGGPAHKLLDPGDVLVRMN 331
             F+ K FDE RRLGL  E E  VR A P  ET MLV + ++P GP+HK L+ GDVL+++N
Sbjct: 301  QFVLKPFDECRRLGLTPEWEAQVRKAFPK-ETNMLVAEIILPEGPSHKKLEEGDVLIKVN 360

Query: 332  GEVITQFLKMETLIDDSVNQTIDIQVERGGTSFTVHLVVQDLHSITPDYFLEVSGAVIHP 391
            G+++TQF+ +E  +D SV QT+ + + RGG    V + V DLH ITPD F+ VSG   H 
Sbjct: 361  GKLLTQFIPLEETLDSSVGQTVKLMLLRGGEEVEVEIEVGDLHQITPDRFVSVSGGSFHN 420

Query: 392  LSYQQARNFRFKCSLVYVTEPGYMLFRAGVPRHAIIKKFAGEEISRVEDLVSVLSKLSRG 451
            LSYQQAR +   C  VYV E G            II+    +E   ++  + V+  +   
Sbjct: 421  LSYQQARLYGVACKGVYVCEAGGSFRFDNNENGWIIQSIDQKETPDLDTFIEVMKGIPDK 480

Query: 452  TRVPLEYISYTDRHRRKSVLVTVDHHEWYAPPQIYVRDDSTGLWIAKPAFQPHLHLQSSP 511
             RV + Y    D H   + ++ VD H W    ++ VR+D TGLW                
Sbjct: 481  ARVVITYKHLRDMHTLHTTVIYVDRH-WAKKMKLAVRNDKTGLW---------------D 540

Query: 512  MTNLGDGYMNQTDVFSDDSSHIRHVHQVNNQEIIDGVVSMETNFEHVSEEARSQDRSDVG 571
             +NL D                           +  V  +      +  E          
Sbjct: 541  FSNLSDA--------------------------LPAVAPVPRKASFIQLE---------- 600

Query: 572  IKKRRVEDDRLTDGNVADCSFHETQEPILEDETAAQTAILRDYQGGTVAAIAANASFPER 631
                                   T  P + D                             
Sbjct: 601  ----------------------NTSHPAVAD----------------------------- 660

Query: 632  IIEPTLVMFEVHVPPS--CMLDGVHSQHFFGTGVIIYHSQSMGLVAVDKNTVAISACDIM 691
                 LV   VHV  +    LDG      +G G++I      GLV + +  V    CDI 
Sbjct: 661  -----LVKSFVHVSVTMPVKLDGFPKNRKWGMGLVI--DAEKGLVIISRAIVPYDLCDIT 720

Query: 692  LSFAAFPIEIPGEVVFLHPVHNYALVAYDPSSLGSVGASVVQAAKLLPGKFLLLLLLFII 751
            ++ A   I + G+VVFLHP+ NYA++ YDP  +     + V++AK               
Sbjct: 721  ITIAD-SIVVEGKVVFLHPLQNYAVIQYDPKLVD----APVRSAK--------------- 780

Query: 752  IIIFCFDYLTLLHKVSLYPSLTEPALQRGDSVYLVGLSRSLQATSRKSIVTNPCAALNIS 811
                                L+   + +G S Y +G +R  +     + VT   A    +
Sbjct: 781  --------------------LSSEMISQGASTYFIGYNRIGRIVHTATTVTEMFAVTIPA 840

Query: 812  SADSPRYRATNMEVIELDTDF-GSTFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQF 871
            ++ +PRYRA N++ I +DT+  G   SGVL  + G VQA+W ++  +     SS  D ++
Sbjct: 841  NSGAPRYRAVNVDAITVDTNLSGQCGSGVLVAQDGTVQALWLTYLGER--NPSSHRDEEY 900

Query: 872  VRGIPIYTISQVLDKIISGSNGPPLLINGVKRPMPVVRILEVELYPTLLSKARSFGLSDE 931
              G+   T+  V+ ++  G               P +R+L  E     +S+AR  G+S+E
Sbjct: 901  HLGLATPTLLPVISQLQQGIT-------------PKLRLLSCEFRAIQMSQARIMGVSEE 960

Query: 932  WVQALVKKDPIRRQVLRV--KGCLAGSKAENLLEQGDMVLAINKQPITCFYDIENACQEL 991
            W+Q +   +    Q+  V  +       A + L++GD++L +N Q IT          EL
Sbjct: 961  WIQKVSLVNTAHHQLFLVTKRTYERNEPAGDHLKEGDILLTLNNQLIT-------KISEL 990

Query: 992  DKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGTTRVINWCGCIVQDPHPAVRALGFLP 1051
            D  Y+ D  L+  I R   E+ + + T   D   T   I++CG I+  PH AVR    + 
Sbjct: 1021 DVMYSHD-YLDAVIVRNTKELHIKLPTVAADDAETDHAISFCGAILHRPHLAVRQQ--IS 990

Query: 1052 EEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKLTPDLDAFVNVTKELEHEQFVRVRTV 1111
            +    VYV+    GSP ++YGL    ++  VNGK TPDL +F++    +    + R++ +
Sbjct: 1081 KLFSEVYVSARTRGSPAYQYGLAPTNFVTHVNGKRTPDLKSFLDAVVGIPDNTYFRLKCM 990

Query: 1112 HLNGKPRVLTLKQNLHYWPTWELRFDPETAM--WRRVT 1142
              +  P V+T+K+N HY+PT EL  DP   +  WRR+T
Sbjct: 1141 TFDSVPWVVTMKKNEHYFPTTELIKDPSEPLTGWRRIT 990

BLAST of MC08g1774 vs. ExPASy Swiss-Prot
Match: Q2H334 (Pro-apoptotic serine protease NMA111 OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=NMA111 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.4e-153
Identity = 372/1110 (33.51%), Postives = 544/1110 (49.01%), Query Frame = 0

Query: 42   DWRKALNKVVPAVVVLRTTACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVA 101
            +W+  + +VV  VV +R     +FDT+ A  S ATGFVVD  RG ILTNRHVV  GP   
Sbjct: 77   EWQATIQRVVRNVVSIRFCQTCSFDTDPALTSEATGFVVDAERGYILTNRHVVGSGPFWG 136

Query: 102  EAMFVNREEVPVRPIYRDPVHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGND 161
              +F N EEV   P+YRDPVHDFG  ++DP AI+++  + +PL P+ A VG+EIRVVGND
Sbjct: 137  YCIFDNHEEVDAYPVYRDPVHDFGILKFDPKAIKYMPVDALPLRPDLAKVGIEIRVVGND 196

Query: 162  SGEKVSILAGTLARLDREAPHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVAL 221
            +GEK+SIL+G ++RLDR AP Y  +GY+DFNT Y QA++   GGSSGSPV++  G AVAL
Sbjct: 197  AGEKLSILSGVISRLDRNAPEY-GEGYSDFNTCYYQASAAASGGSSGSPVVNMDGYAVAL 256

Query: 222  NAGSKSSSASA-FFLPLERVVRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEI 281
             AG ++  A+  +FLPL+R +RAL  LQ+G            I RG +Q  F+ K FDE 
Sbjct: 257  QAGGRADGAATDYFLPLDRPLRALKCLQEGN----------PITRGDIQCQFVLKPFDEC 316

Query: 282  RRLGLRSETEQMVRLASPPGETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKM 341
            RRLGL  E E  +R A P  ET MLV + V+P GP+HK  + GDVL+++NGE++TQF+++
Sbjct: 317  RRLGLTPEWEAQIRKAFPK-ETNMLVAEIVLPEGPSHKKAEEGDVLIKVNGELLTQFIRL 376

Query: 342  ETLIDDSVNQTIDIQVERGGTSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFR 401
            + ++D SV + + + + RGG    V + V DLHSITPD F+ V+G   H LSYQQAR + 
Sbjct: 377  DDILDSSVGKPVKLLLLRGGEEIEVEIEVGDLHSITPDRFVSVAGGSFHSLSYQQARLYG 436

Query: 402  FKCSLVYVTEPGYMLFRAGVPRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISY 461
              C  V+V E G            +I+    ++   +E  + V+  +    RV + Y   
Sbjct: 437  VACKGVFVCEAGGSFRFDNAENGWLIQTVDHKKTPDLETFIEVMKGIHDKARVVVTYKHL 496

Query: 462  TDRHRRKSVLVTVDHHEWYAPPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMN 521
             D H   + ++ +D H W    ++ VR+D TGLW                 TNL D    
Sbjct: 497  RDLHTLNTTILHIDRH-WSKKMKLAVRNDETGLW---------------DFTNLADP--- 556

Query: 522  QTDVFSDDSSHIRHVHQVNNQEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDR 581
                                   +  V  +    + +  E                    
Sbjct: 557  -----------------------LPPVAPIPRKADFIQLE-------------------- 616

Query: 582  LTDGNVADCSFHETQEPILEDETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFE 641
                         T  P + D                                  LV   
Sbjct: 617  ------------HTSHPAVAD----------------------------------LVRSF 676

Query: 642  VHVPPSCM----LDGVHSQHFFGTGVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPI 701
            VHV  SC+    LDG      +G G++I      GLV + +  V    CDI ++ A   I
Sbjct: 677  VHV--SCVMPVKLDGFPKNRKWGMGLVI--DADKGLVVISRAIVPYDLCDITVTIAD-SI 736

Query: 702  EIPGEVVFLHPVHNYALVAYDPSSLGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDY 761
             + G+VVFLHP+ NYA++ YDP     V A V+ A                         
Sbjct: 737  VVEGKVVFLHPLQNYAIIQYDPK---LVDAPVLSA------------------------- 796

Query: 762  LTLLHKVSLYPSLTEPALQRGDSVYLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYR 821
                        L+   + +G S Y +G +R  +     + VT   A    +++ +PRYR
Sbjct: 797  -----------RLSSQEITQGASTYFIGYNRIGRIVHAATTVTEIFAVTIPANSGAPRYR 856

Query: 822  ATNMEVIELDTDF-GSTFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYT 881
            A N++ I +DT   G   SGVL  + G VQA+W ++  +     S+  D ++  G+   T
Sbjct: 857  AVNVDAITVDTSLSGQCGSGVLVAQDGTVQALWLTYLGER--NPSTHRDEEYHLGLATPT 916

Query: 882  ISQVLDKIISGSNGPPLLINGVKRPMPVVRILEVELYPTLLSKARSFGLSDEWVQALVKK 941
            +  V+++I  G +             P +R+L VE     +S+AR  G+S+EW+Q +   
Sbjct: 917  LLPVVEQIQRGVD-------------PKLRMLSVEFRAIQMSQARLMGVSEEWIQKVSVA 976

Query: 942  DPIRRQVLRV-KGCLAGSKAEN--LLEQGDMVLAINKQPITCFYDIENACQELDKNYTTD 1001
            +    Q+  V K     ++ E    L +GD+VL++N + IT   D       LD  Y+ +
Sbjct: 977  NTAHHQLFMVTKRTFERNEQEEAAALLEGDVVLSLNGKIITKISD-------LDIMYSNE 997

Query: 1002 GKLNMTIFRQGHEIDLLVGTDVRDGNGTTRVINWCGCIVQDPHPAVRALGFLPEEGHGVY 1061
             +L+  + R   E+ L + T   D   TTR +++CG I   PH AVR    + +    VY
Sbjct: 1037 -QLDAVLVRNCEELSLKLDTVAADDVETTRAVSFCGAIFHAPHHAVRQQ--ISKLFSEVY 997

Query: 1062 VARWCHGSPVHRYGLYALQWIVEVNGKLTPDLDAFVNVTKELEHEQFVRVRTVHLNGKPR 1121
            V+    GSP ++YGL    +I  VNGK TPDL+AF+    ++    + R+R +  +  P 
Sbjct: 1097 VSARTRGSPSYQYGLAPTNFITHVNGKPTPDLEAFLAEVVKIPDNTYFRLRAMSFDSVPW 997

Query: 1122 VLTLKQNLHYWPTWELRFDP-ETAMWRRVT 1142
            V+T+K+N HY+PT EL  DP E   WRR+T
Sbjct: 1157 VVTMKKNDHYFPTMELIKDPKEECGWRRIT 997

BLAST of MC08g1774 vs. ExPASy Swiss-Prot
Match: A1DP85 (Pro-apoptotic serine protease nma111 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=nma111 PE=3 SV=2)

HSP 1 Score: 543.1 bits (1398), Expect = 7.5e-153
Identity = 379/1143 (33.16%), Postives = 559/1143 (48.91%), Query Frame = 0

Query: 7    TLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTTACRAFD 66
            T G  + A G     +DE       P+    A + +W+  + KVV +VV +      +FD
Sbjct: 35   TPGDATPANGTVYDVEDEEDTGRVMPIGPAQADSPEWQATIEKVVKSVVSIHFCQTCSFD 94

Query: 67   TESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDPVHDFGF 126
            T+ + +S ATGFVVD  RG ILTNRHVV  GP     +F N EE  VRP+YRDPVHDFG 
Sbjct: 95   TDLSMSSQATGFVVDAERGYILTNRHVVCAGPFWGYCIFDNHEECDVRPVYRDPVHDFGI 154

Query: 127  FRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREAPHYKKD 186
             ++DP AI+++   E+ L PEAA VG EIRVVGND+GEK+SIL+G ++RLDR AP Y  D
Sbjct: 155  LKFDPKAIRYMELTELKLRPEAARVGCEIRVVGNDAGEKLSILSGVISRLDRNAPEY-GD 214

Query: 187  GYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASA-FFLPLERVVRALT 246
            GY DFNT Y+QAA+   GGSSGSPV++  G A+AL AG ++  A+  +FLPL+R +RAL 
Sbjct: 215  GYCDFNTNYIQAAAAASGGSSGSPVVNIDGHAIALQAGGRADGAATDYFLPLDRPLRALE 274

Query: 247  FLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPGETGML 306
             +++G            + RGT+Q  ++ K FDE RRLGL  E E  VR AS P ET ML
Sbjct: 275  CIRRGE----------PVARGTIQTQWILKPFDECRRLGLTPEWEAAVRKAS-PHETSML 334

Query: 307  VVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGGTSFTV 366
            V + ++P GPA   L+ GDVL+++NGE++TQF++++ ++D SV +T+ + V+RGG +  V
Sbjct: 335  VAEIILPEGPADGKLEEGDVLLQVNGELLTQFIRLDDILDSSVGKTVRLLVQRGGQNVEV 394

Query: 367  HLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGVPRHAI 426
               V DLH+ITPD F+ V+G   H LSYQQAR +      VYV E              I
Sbjct: 395  ECEVGDLHAITPDRFVTVAGGTFHNLSYQQARLYAIAARGVYVCEAAGSFKLENTLSGWI 454

Query: 427  IKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYAPPQIY 486
            I          +++   V+  +    RV + Y    D H R + +V +D H W+   ++ 
Sbjct: 455  IDAVDKRPTRNLDEFTEVMKTIPDRARVVISYRHIRDLHTRGTSIVYIDRH-WHPKMRMA 514

Query: 487  VRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNNQEIID 546
            +R+D TGLW                 ++L D    +T V                     
Sbjct: 515  IRNDETGLW---------------DFSDLADPVPAETPV--------------------- 574

Query: 547  GVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILEDETAA 606
                                         R  D    DG           +P + D    
Sbjct: 575  ----------------------------PRKADFIQLDG---------VSQPAVAD---- 634

Query: 607  QTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGTGVIIY 666
               I+R +       +  + + P +                  LDG       G G++I 
Sbjct: 635  ---IVRSF-------VRVSCTMPLK------------------LDGYPQAKKTGFGLVI- 694

Query: 667  HSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSSLGSVG 726
                 GLV V +  V  + CDI ++  A  I +  +V+FLHP+ NY ++ YDPS +    
Sbjct: 695  -DAEKGLVVVSRAIVPYNLCDINIT-VADSIIVAAKVIFLHPLQNYTIIQYDPSLV---- 754

Query: 727  ASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSVYLVGL 786
             + VQ+AK                                   L+   +++G     VG 
Sbjct: 755  QAPVQSAK-----------------------------------LSTEYIKQGQETIFVGF 814

Query: 787  SRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDF-GSTFSGVLTDEHGRV 846
            +++ +    K+ VT+       ++A +PRYRA N++ I +DT   G   +GVL  E G V
Sbjct: 815  NQNFRIVVAKTAVTDITTVSIPANASAPRYRAINLDAITVDTGLSGQCTNGVLIGEDGVV 874

Query: 847  QAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRPMPVV 906
            QA+W ++  +     SS +D ++  G     +  V  KI  G              +P +
Sbjct: 875  QALWLNYLGERT--PSSHKDVEYHLGFATPALLPVTSKIQQGI-------------IPKL 934

Query: 907  RILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVK--GCLAGSKAENL--LEQ 966
            RIL +E Y   +S+AR  G+S+EW+Q + + +P R Q+  V+   C      +N   L++
Sbjct: 935  RILNMESYVVQMSQARIMGVSEEWIQKVAQANPSRHQLFMVRKVDCPPPQFTDNADSLQE 991

Query: 967  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1026
            GD++L ++ Q IT          ELDK Y  +  L+  I R G E+ L + T   +   T
Sbjct: 995  GDIILTLDGQLIT-------RVSELDKMYEKE-VLDALIVRNGQEMHLKLPTVPTEDLET 991

Query: 1027 TRVINWCGCIVQDPHPAVR-ALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGK 1086
             R + +CG ++Q PH AVR  +  L  E   VYV+    GSP ++YGL    +I  VNG 
Sbjct: 1055 DRAVVFCGAVLQKPHHAVRQQISKLHSE---VYVSARSRGSPAYQYGLAPTNFITAVNGV 991

Query: 1087 LTPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAM-WR 1142
             TP+LD+FV    ++    + R+R V  +  P V+T+K+N HY+P  E   DP   + WR
Sbjct: 1115 PTPNLDSFVREVSKIPDNTYFRLRAVTFDNVPWVVTMKKNDHYFPMSEYVKDPSQPLGWR 991

BLAST of MC08g1774 vs. NCBI nr
Match: XP_022143406.1 (protease Do-like 7 [Momordica charantia])

HSP 1 Score: 2226 bits (5767), Expect = 0.0
Identity = 1116/1150 (97.04%), Postives = 1116/1150 (97.04%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT
Sbjct: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG
Sbjct: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
            TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV
Sbjct: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA
Sbjct: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN
Sbjct: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
            QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE
Sbjct: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSV 780
            LGSVGASVVQAAKLLP                                  EPALQRGDSV
Sbjct: 721  LGSVGASVVQAAKLLP----------------------------------EPALQRGDSV 780

Query: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840
            YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE
Sbjct: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840

Query: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900
            HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP
Sbjct: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900

Query: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960
            MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ
Sbjct: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960

Query: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020
            GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT
Sbjct: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020

Query: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080
            TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL
Sbjct: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080

Query: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1140
            TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV
Sbjct: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1116

Query: 1141 TIKALNPGNS 1150
            TIKALNPGNS
Sbjct: 1141 TIKALNPGNS 1116

BLAST of MC08g1774 vs. NCBI nr
Match: XP_022998710.1 (protease Do-like 7 [Cucurbita maxima])

HSP 1 Score: 2112 bits (5473), Expect = 0.0
Identity = 1054/1149 (91.73%), Postives = 1085/1149 (94.43%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MAD +E+LGSESAAIGIDST KDELCMEIDPP R+NLATADDWRKAL+KVVPAVVVLRTT
Sbjct: 1    MADASESLGSESAAIGIDSTTKDELCMEIDPPFRENLATADDWRKALSKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQKGRDSY NKWEAVSIPRGTLQATFLHKGFDEIRRLGL+SETEQMVRLASPP 
Sbjct: 241  VRALTFLQKGRDSYDNKWEAVSIPRGTLQATFLHKGFDEIRRLGLQSETEQMVRLASPPS 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLL+PGDVLV +NGEVITQFLKMETL+DDSV QTID+QVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLEPGDVLVCVNGEVITQFLKMETLLDDSVKQTIDLQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
            TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKC LVYVTEPGYMLFRAGV
Sbjct: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCGLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEI R+ DLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVD+HEWYA
Sbjct: 421  PRHAIIKKFAGEEILRIGDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDNHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVRDDSTGLWIAKPA QPH+HLQSSPMTN+G+GYMNQ+ + +DDSSHIRHVHQVNN
Sbjct: 481  PPQIYVRDDSTGLWIAKPAIQPHVHLQSSPMTNVGEGYMNQSVLLNDDSSHIRHVHQVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
             EIIDGVVSMETNFEHVSEEARSQDRSD G KKRRVEDDRLTDGN+AD SF ETQE ILE
Sbjct: 541  PEIIDGVVSMETNFEHVSEEARSQDRSDAGTKKRRVEDDRLTDGNIADSSFLETQETILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            D TA QTA+LRDYQGGTVAA+AANASFPE IIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DATATQTAVLRDYQGGTVAAVAANASFPEHIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHS SMGLVAVDKNTVAIS+CDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSHSMGLVAVDKNTVAISSCDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSV 780
            LGSVGAS VQAA+LLP                                  EPAL+RGDSV
Sbjct: 721  LGSVGASAVQAAELLP----------------------------------EPALRRGDSV 780

Query: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840
            YLVGLSRSLQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDTDFGSTFSGVLTDE
Sbjct: 781  YLVGLSRSLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840

Query: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900
            HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKI+SG+NGPPLLINGVKRP
Sbjct: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKILSGANGPPLLINGVKRP 900

Query: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960
            MP+VRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ
Sbjct: 901  MPLVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960

Query: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020
            GDMVLAINKQPITCFYDIENACQELD+  +TDGKLNMTIFRQGHEIDLLVGTDVRDGNGT
Sbjct: 961  GDMVLAINKQPITCFYDIENACQELDRINSTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020

Query: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080
            TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL
Sbjct: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080

Query: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1140
            TPDL+ FVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDP+TAMWRRV
Sbjct: 1081 TPDLETFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPDTAMWRRV 1115

Query: 1141 TIKALNPGN 1149
            TIKAL+  N
Sbjct: 1141 TIKALDSNN 1115

BLAST of MC08g1774 vs. NCBI nr
Match: KAG6607006.1 (Protease Do-like 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2112 bits (5471), Expect = 0.0
Identity = 1054/1149 (91.73%), Postives = 1084/1149 (94.34%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MAD +E+LGSESAAIGIDST KDELCMEIDPP R+NLATADDWRKAL+KVVPAVVVLRTT
Sbjct: 1    MADASESLGSESAAIGIDSTTKDELCMEIDPPFRENLATADDWRKALSKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNY+EIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYDEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQKGRDSY NKWEAVSIPRGTLQATFLHKGFDEIRRLGL+SETEQMVRLASPP 
Sbjct: 241  VRALTFLQKGRDSYDNKWEAVSIPRGTLQATFLHKGFDEIRRLGLQSETEQMVRLASPPS 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLL+PGDVLV +NGEVITQFLKMETL+DDSV QTID+QVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLEPGDVLVCVNGEVITQFLKMETLLDDSVKQTIDLQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
            TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKC LVYVTEPGYMLFRAGV
Sbjct: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCGLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEI R+ DLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVD+HEWYA
Sbjct: 421  PRHAIIKKFAGEEILRIGDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDNHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVRDDSTGLWIAKPA QPH+HLQSSPMTN+G+GYMNQ+ + +DDSSHIRHVHQVNN
Sbjct: 481  PPQIYVRDDSTGLWIAKPAIQPHVHLQSSPMTNVGEGYMNQSVLLNDDSSHIRHVHQVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
             EIIDGVVSMETNFEHVSEEARSQDRSD G KKRRVEDDRLTDGN AD SF ETQE ILE
Sbjct: 541  PEIIDGVVSMETNFEHVSEEARSQDRSDAGTKKRRVEDDRLTDGNTADSSFLETQETILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            D TA QTA+LRDYQGGTVAA+AANASFPE IIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DATATQTAVLRDYQGGTVAAVAANASFPEHIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHS SMGLVAVDKNTVAIS+CDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSHSMGLVAVDKNTVAISSCDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSV 780
            LGSVGAS VQAAKLLP                                  EPAL+RGDSV
Sbjct: 721  LGSVGASAVQAAKLLP----------------------------------EPALRRGDSV 780

Query: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840
            YLVGLSRSLQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDTDFGSTFSGVLTDE
Sbjct: 781  YLVGLSRSLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840

Query: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900
            HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKI+SG+NGPPLLINGVKRP
Sbjct: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKILSGANGPPLLINGVKRP 900

Query: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960
            MP+VRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ
Sbjct: 901  MPLVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960

Query: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020
            GDMVLAINKQPITCFYDIENACQELD+  +TDGKLNMTIFRQGHEIDLLVGTDVRDGNGT
Sbjct: 961  GDMVLAINKQPITCFYDIENACQELDRINSTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020

Query: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080
            TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL
Sbjct: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080

Query: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1140
            TPDL+ FVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDP+TAMWRRV
Sbjct: 1081 TPDLETFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPDTAMWRRV 1115

Query: 1141 TIKALNPGN 1149
            TIKAL+  N
Sbjct: 1141 TIKALDSSN 1115

BLAST of MC08g1774 vs. NCBI nr
Match: XP_023523660.1 (protease Do-like 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2111 bits (5469), Expect = 0.0
Identity = 1053/1149 (91.64%), Postives = 1085/1149 (94.43%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MAD +E+LGSESAAIGIDST KDELCMEIDPP R+NLATADDWRKAL+KVVPAVVVLRTT
Sbjct: 1    MADASESLGSESAAIGIDSTTKDELCMEIDPPFRENLATADDWRKALSKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNY+EIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYDEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQKGRDSY NKWEAVSIPRGTLQATFLHKGFDEIRRLGL+SETEQMVRLASPP 
Sbjct: 241  VRALTFLQKGRDSYDNKWEAVSIPRGTLQATFLHKGFDEIRRLGLQSETEQMVRLASPPS 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLL+PGDVLV +NGEVITQFLKMETL+DDSV QTID+QVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLEPGDVLVCVNGEVITQFLKMETLLDDSVKQTIDLQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
            TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKC LVYVTEPGYMLFRAGV
Sbjct: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCGLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEI R+ DLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVD+HEWYA
Sbjct: 421  PRHAIIKKFAGEEILRIGDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDNHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVRDDSTGLWIAKPA QPH+HLQSSPMTN+G+GYMNQ+ + +DDSSHIRHVHQVNN
Sbjct: 481  PPQIYVRDDSTGLWIAKPAIQPHVHLQSSPMTNVGEGYMNQSVLLNDDSSHIRHVHQVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
             EIIDGVVSMETNFEHVSEEARSQDRSD G KKRRVEDDRLTDGN+AD SF ETQE ILE
Sbjct: 541  PEIIDGVVSMETNFEHVSEEARSQDRSDAGTKKRRVEDDRLTDGNIADSSFLETQETILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            D TA QTA+LRDYQGGTVAA+AANASFPE IIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DATATQTAVLRDYQGGTVAAVAANASFPEHIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHS SMGLVAVDKNTVAIS+CDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSHSMGLVAVDKNTVAISSCDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSV 780
            LGSVGAS VQAAKLLP                                  EPAL+RGDSV
Sbjct: 721  LGSVGASAVQAAKLLP----------------------------------EPALRRGDSV 780

Query: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840
            YLVGLSRSLQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDTDFGSTFSGVLTDE
Sbjct: 781  YLVGLSRSLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840

Query: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900
            HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKI+SG+NGPPLLINGVKRP
Sbjct: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKILSGANGPPLLINGVKRP 900

Query: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960
            MP+VRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ
Sbjct: 901  MPLVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960

Query: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020
            GDMVLAINKQPITCFYDIENACQELD+  +TDGKL+MTIFRQGHEIDLLVGTDVRDGNGT
Sbjct: 961  GDMVLAINKQPITCFYDIENACQELDRINSTDGKLDMTIFRQGHEIDLLVGTDVRDGNGT 1020

Query: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080
            TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL
Sbjct: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080

Query: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1140
            TPDL+ FVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDP+TAMWRRV
Sbjct: 1081 TPDLETFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPDTAMWRRV 1115

Query: 1141 TIKALNPGN 1149
            TIKAL+  N
Sbjct: 1141 TIKALDSSN 1115

BLAST of MC08g1774 vs. NCBI nr
Match: XP_008457070.1 (PREDICTED: protease Do-like 7 isoform X1 [Cucumis melo])

HSP 1 Score: 2109 bits (5464), Expect = 0.0
Identity = 1058/1162 (91.05%), Postives = 1092/1162 (93.98%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MADP+E LGS+SAAIGI ST KD+LCMEIDPP R+NLATADDWRKALNKVVPAVVVLRTT
Sbjct: 1    MADPSEGLGSDSAAIGIHSTTKDDLCMEIDPPFRENLATADDWRKALNKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNYEEI LAPEAA VGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYEEITLAPEAAYVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQ GRD Y +KWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVR ASP G
Sbjct: 241  VRALTFLQMGRDCYDHKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRRASPSG 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLL+PGDVLVRMNGEVITQFLKMETL+DD+V QTID+QVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLEPGDVLVRMNGEVITQFLKMETLLDDTVKQTIDLQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
             SFTVHLVVQDLHSITPDYFLEV GAVIHPLSYQQARNFRFKC LVYVTEPGYMLFRAGV
Sbjct: 361  ASFTVHLVVQDLHSITPDYFLEVGGAVIHPLSYQQARNFRFKCGLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA
Sbjct: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVR+D+TGLWIAKPAFQPHL L+SSPMTN+G+GYMN TDV SDDSSH+RH+H VNN
Sbjct: 481  PPQIYVRNDTTGLWIAKPAFQPHLRLESSPMTNVGEGYMNPTDVLSDDSSHLRHMHPVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
             EIIDGVVSMETNFEH SEEARSQDRSD G KKRRVEDDRLTDGNVAD SFHETQE ILE
Sbjct: 541  LEIIDGVVSMETNFEHGSEEARSQDRSDAGTKKRRVEDDRLTDGNVADSSFHETQETILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            D TA QTA +RDYQGGTVA +AANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DATAMQTANIRDYQGGTVAVVAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHS +MGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSHNMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFL-------------LLLLLFIIIIIFCFDYLTLLHKVSLYP 780
            LGSVGA+ VQAAKLLPG  L             LLL++++    + FD      K   + 
Sbjct: 721  LGSVGAAAVQAAKLLPGHLLREEKAFVGPCVRALLLVVWLERNNWAFD-----DKAQAF- 780

Query: 781  SLTEPALQRGDSVYLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDT 840
               EPAL+RGDSVYLVGLSRSLQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDT
Sbjct: 781  ---EPALRRGDSVYLVGLSRSLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDT 840

Query: 841  DFGSTFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGS 900
            DFGSTFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKI+SG+
Sbjct: 841  DFGSTFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKILSGA 900

Query: 901  NGPPLLINGVKRPMPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKG 960
            NGPPLLINGVKRPMP+VRILEVELYPTLLSKARSFGLSDEWVQ LVKKDPIRRQVLRVKG
Sbjct: 901  NGPPLLINGVKRPMPLVRILEVELYPTLLSKARSFGLSDEWVQDLVKKDPIRRQVLRVKG 960

Query: 961  CLAGSKAENLLEQGDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEID 1020
            CLAGSKAENLLEQGDMVLAINKQPITCFYDIENACQELDKN +TDGKLNMTIFRQGHEID
Sbjct: 961  CLAGSKAENLLEQGDMVLAINKQPITCFYDIENACQELDKNNSTDGKLNMTIFRQGHEID 1020

Query: 1021 LLVGTDVRDGNGTTRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGL 1080
            LLVGTDVRDGNGTTR+INWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGL
Sbjct: 1021 LLVGTDVRDGNGTTRIINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGL 1080

Query: 1081 YALQWIVEVNGKLTPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWE 1140
            YALQWIVEVNGKLTPDLD FVNVTKELEHE+FVRVRTVHLNGKPRVLTLKQNLHYWPTWE
Sbjct: 1081 YALQWIVEVNGKLTPDLDTFVNVTKELEHEEFVRVRTVHLNGKPRVLTLKQNLHYWPTWE 1140

Query: 1141 LRFDPETAMWRRVTIKALNPGN 1149
            LRFDP+TAMWRRVTIKALN  N
Sbjct: 1141 LRFDPDTAMWRRVTIKALNSNN 1153

BLAST of MC08g1774 vs. ExPASy TrEMBL
Match: A0A6J1CQ60 (protease Do-like 7 OS=Momordica charantia OX=3673 GN=LOC111013286 PE=3 SV=1)

HSP 1 Score: 2226 bits (5767), Expect = 0.0
Identity = 1116/1150 (97.04%), Postives = 1116/1150 (97.04%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT
Sbjct: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG
Sbjct: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
            TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV
Sbjct: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA
Sbjct: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN
Sbjct: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
            QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE
Sbjct: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSV 780
            LGSVGASVVQAAKLLP                                  EPALQRGDSV
Sbjct: 721  LGSVGASVVQAAKLLP----------------------------------EPALQRGDSV 780

Query: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840
            YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE
Sbjct: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840

Query: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900
            HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP
Sbjct: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900

Query: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960
            MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ
Sbjct: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960

Query: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020
            GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT
Sbjct: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020

Query: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080
            TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL
Sbjct: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080

Query: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1140
            TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV
Sbjct: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1116

Query: 1141 TIKALNPGNS 1150
            TIKALNPGNS
Sbjct: 1141 TIKALNPGNS 1116

BLAST of MC08g1774 vs. ExPASy TrEMBL
Match: A0A6J1KD90 (Pro-apoptotic serine protease NMA111 OS=Cucurbita maxima OX=3661 GN=LOC111493293 PE=3 SV=1)

HSP 1 Score: 2112 bits (5473), Expect = 0.0
Identity = 1054/1149 (91.73%), Postives = 1085/1149 (94.43%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MAD +E+LGSESAAIGIDST KDELCMEIDPP R+NLATADDWRKAL+KVVPAVVVLRTT
Sbjct: 1    MADASESLGSESAAIGIDSTTKDELCMEIDPPFRENLATADDWRKALSKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQKGRDSY NKWEAVSIPRGTLQATFLHKGFDEIRRLGL+SETEQMVRLASPP 
Sbjct: 241  VRALTFLQKGRDSYDNKWEAVSIPRGTLQATFLHKGFDEIRRLGLQSETEQMVRLASPPS 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLL+PGDVLV +NGEVITQFLKMETL+DDSV QTID+QVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLEPGDVLVCVNGEVITQFLKMETLLDDSVKQTIDLQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
            TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKC LVYVTEPGYMLFRAGV
Sbjct: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCGLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEI R+ DLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVD+HEWYA
Sbjct: 421  PRHAIIKKFAGEEILRIGDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDNHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVRDDSTGLWIAKPA QPH+HLQSSPMTN+G+GYMNQ+ + +DDSSHIRHVHQVNN
Sbjct: 481  PPQIYVRDDSTGLWIAKPAIQPHVHLQSSPMTNVGEGYMNQSVLLNDDSSHIRHVHQVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
             EIIDGVVSMETNFEHVSEEARSQDRSD G KKRRVEDDRLTDGN+AD SF ETQE ILE
Sbjct: 541  PEIIDGVVSMETNFEHVSEEARSQDRSDAGTKKRRVEDDRLTDGNIADSSFLETQETILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            D TA QTA+LRDYQGGTVAA+AANASFPE IIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DATATQTAVLRDYQGGTVAAVAANASFPEHIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHS SMGLVAVDKNTVAIS+CDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSHSMGLVAVDKNTVAISSCDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSV 780
            LGSVGAS VQAA+LLP                                  EPAL+RGDSV
Sbjct: 721  LGSVGASAVQAAELLP----------------------------------EPALRRGDSV 780

Query: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840
            YLVGLSRSLQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDTDFGSTFSGVLTDE
Sbjct: 781  YLVGLSRSLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840

Query: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900
            HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKI+SG+NGPPLLINGVKRP
Sbjct: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKILSGANGPPLLINGVKRP 900

Query: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960
            MP+VRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ
Sbjct: 901  MPLVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960

Query: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020
            GDMVLAINKQPITCFYDIENACQELD+  +TDGKLNMTIFRQGHEIDLLVGTDVRDGNGT
Sbjct: 961  GDMVLAINKQPITCFYDIENACQELDRINSTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020

Query: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080
            TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL
Sbjct: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080

Query: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1140
            TPDL+ FVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDP+TAMWRRV
Sbjct: 1081 TPDLETFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPDTAMWRRV 1115

Query: 1141 TIKALNPGN 1149
            TIKAL+  N
Sbjct: 1141 TIKALDSNN 1115

BLAST of MC08g1774 vs. ExPASy TrEMBL
Match: A0A1S3C5Y4 (Pro-apoptotic serine protease NMA111 OS=Cucumis melo OX=3656 GN=LOC103496840 PE=3 SV=1)

HSP 1 Score: 2109 bits (5464), Expect = 0.0
Identity = 1058/1162 (91.05%), Postives = 1092/1162 (93.98%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MADP+E LGS+SAAIGI ST KD+LCMEIDPP R+NLATADDWRKALNKVVPAVVVLRTT
Sbjct: 1    MADPSEGLGSDSAAIGIHSTTKDDLCMEIDPPFRENLATADDWRKALNKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNYEEI LAPEAA VGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYEEITLAPEAAYVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQ GRD Y +KWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVR ASP G
Sbjct: 241  VRALTFLQMGRDCYDHKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRRASPSG 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLL+PGDVLVRMNGEVITQFLKMETL+DD+V QTID+QVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLEPGDVLVRMNGEVITQFLKMETLLDDTVKQTIDLQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
             SFTVHLVVQDLHSITPDYFLEV GAVIHPLSYQQARNFRFKC LVYVTEPGYMLFRAGV
Sbjct: 361  ASFTVHLVVQDLHSITPDYFLEVGGAVIHPLSYQQARNFRFKCGLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA
Sbjct: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVR+D+TGLWIAKPAFQPHL L+SSPMTN+G+GYMN TDV SDDSSH+RH+H VNN
Sbjct: 481  PPQIYVRNDTTGLWIAKPAFQPHLRLESSPMTNVGEGYMNPTDVLSDDSSHLRHMHPVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
             EIIDGVVSMETNFEH SEEARSQDRSD G KKRRVEDDRLTDGNVAD SFHETQE ILE
Sbjct: 541  LEIIDGVVSMETNFEHGSEEARSQDRSDAGTKKRRVEDDRLTDGNVADSSFHETQETILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            D TA QTA +RDYQGGTVA +AANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DATAMQTANIRDYQGGTVAVVAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHS +MGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSHNMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFL-------------LLLLLFIIIIIFCFDYLTLLHKVSLYP 780
            LGSVGA+ VQAAKLLPG  L             LLL++++    + FD      K   + 
Sbjct: 721  LGSVGAAAVQAAKLLPGHLLREEKAFVGPCVRALLLVVWLERNNWAFD-----DKAQAF- 780

Query: 781  SLTEPALQRGDSVYLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDT 840
               EPAL+RGDSVYLVGLSRSLQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDT
Sbjct: 781  ---EPALRRGDSVYLVGLSRSLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDT 840

Query: 841  DFGSTFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGS 900
            DFGSTFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKI+SG+
Sbjct: 841  DFGSTFSGVLTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKILSGA 900

Query: 901  NGPPLLINGVKRPMPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKG 960
            NGPPLLINGVKRPMP+VRILEVELYPTLLSKARSFGLSDEWVQ LVKKDPIRRQVLRVKG
Sbjct: 901  NGPPLLINGVKRPMPLVRILEVELYPTLLSKARSFGLSDEWVQDLVKKDPIRRQVLRVKG 960

Query: 961  CLAGSKAENLLEQGDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEID 1020
            CLAGSKAENLLEQGDMVLAINKQPITCFYDIENACQELDKN +TDGKLNMTIFRQGHEID
Sbjct: 961  CLAGSKAENLLEQGDMVLAINKQPITCFYDIENACQELDKNNSTDGKLNMTIFRQGHEID 1020

Query: 1021 LLVGTDVRDGNGTTRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGL 1080
            LLVGTDVRDGNGTTR+INWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGL
Sbjct: 1021 LLVGTDVRDGNGTTRIINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGL 1080

Query: 1081 YALQWIVEVNGKLTPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWE 1140
            YALQWIVEVNGKLTPDLD FVNVTKELEHE+FVRVRTVHLNGKPRVLTLKQNLHYWPTWE
Sbjct: 1081 YALQWIVEVNGKLTPDLDTFVNVTKELEHEEFVRVRTVHLNGKPRVLTLKQNLHYWPTWE 1140

Query: 1141 LRFDPETAMWRRVTIKALNPGN 1149
            LRFDP+TAMWRRVTIKALN  N
Sbjct: 1141 LRFDPDTAMWRRVTIKALNSNN 1153

BLAST of MC08g1774 vs. ExPASy TrEMBL
Match: A0A6J1G9G5 (Pro-apoptotic serine protease NMA111 OS=Cucurbita moschata OX=3662 GN=LOC111452157 PE=3 SV=1)

HSP 1 Score: 2107 bits (5460), Expect = 0.0
Identity = 1051/1149 (91.47%), Postives = 1084/1149 (94.34%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MAD +E+LGSESAAIGIDST KDELCMEIDPP R+NLATADDWRKAL+KVVPAVVVLRTT
Sbjct: 1    MADASESLGSESAAIGIDSTTKDELCMEIDPPFRENLATADDWRKALSKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNY+EIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYDEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQKGRDSY NKWEAVSIPRGTLQATFLHKGFDEIRRLGL+SETEQMVRLASPP 
Sbjct: 241  VRALTFLQKGRDSYDNKWEAVSIPRGTLQATFLHKGFDEIRRLGLQSETEQMVRLASPPS 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLL+PGDVLV +NGEVITQFLKMETL+DDSV QTID+QVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLEPGDVLVCVNGEVITQFLKMETLLDDSVKQTIDLQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
            TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKC LVYVTEPGYMLFRAGV
Sbjct: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCGLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEI R+ DLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVD+HEWYA
Sbjct: 421  PRHAIIKKFAGEEILRIGDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDNHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVRDDSTGLWIAKPA QPH+HLQSSPMTN+G+GYMNQ+ + +DDSSHIRHVHQVNN
Sbjct: 481  PPQIYVRDDSTGLWIAKPAIQPHVHLQSSPMTNVGEGYMNQSVLLNDDSSHIRHVHQVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
             EIIDGVVSMETNFE+VSEEARSQDRSD G KKRR+EDDRLTDGN+AD SF ETQE ILE
Sbjct: 541  PEIIDGVVSMETNFEYVSEEARSQDRSDAGTKKRRMEDDRLTDGNIADSSFLETQETILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            D TA QTA+LRDYQGGTVAA+AANASFPE IIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DATATQTAVLRDYQGGTVAAVAANASFPEHIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHS SMGLVAVDKNTVAIS+CDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSHSMGLVAVDKNTVAISSCDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSV 780
            LGSVGAS VQAAKLLP                                  EPAL+RGDSV
Sbjct: 721  LGSVGASAVQAAKLLP----------------------------------EPALRRGDSV 780

Query: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840
            YLVGLSRSLQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDTDFGSTFSGVLTDE
Sbjct: 781  YLVGLSRSLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840

Query: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900
            HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKI+SG+NGPPLLINGVKRP
Sbjct: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKILSGANGPPLLINGVKRP 900

Query: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960
            MP+VRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ
Sbjct: 901  MPLVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960

Query: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020
            GDMVLAINKQPITCFYDIENACQELD+  +TDGKLNMTIFRQGHEIDLLVGTDVRDGNGT
Sbjct: 961  GDMVLAINKQPITCFYDIENACQELDRINSTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020

Query: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080
            TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL
Sbjct: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080

Query: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1140
            TPDL+ FVNV KELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDP+TAMWRRV
Sbjct: 1081 TPDLETFVNVAKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPDTAMWRRV 1115

Query: 1141 TIKALNPGN 1149
            TIKAL+  N
Sbjct: 1141 TIKALDSNN 1115

BLAST of MC08g1774 vs. ExPASy TrEMBL
Match: A0A1S3C490 (Pro-apoptotic serine protease NMA111 OS=Cucumis melo OX=3656 GN=LOC103496840 PE=3 SV=1)

HSP 1 Score: 2106 bits (5457), Expect = 0.0
Identity = 1050/1149 (91.38%), Postives = 1078/1149 (93.82%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            MADP+E LGS+SAAIGI ST KD+LCMEIDPP R+NLATADDWRKALNKVVPAVVVLRTT
Sbjct: 1    MADPSEGLGSDSAAIGIHSTTKDDLCMEIDPPFRENLATADDWRKALNKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP
Sbjct: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFFRYDPGAIQFLNYEEI LAPEAA VGLEIRVVGNDSGEKVSILAGTLARLDREA
Sbjct: 121  VHDFGFFRYDPGAIQFLNYEEITLAPEAAYVGLEIRVVGNDSGEKVSILAGTLARLDREA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRALTFLQ GRD Y +KWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVR ASP G
Sbjct: 241  VRALTFLQMGRDCYDHKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRRASPSG 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVPGGPAHKLL+PGDVLVRMNGEVITQFLKMETL+DD+V QTID+QVERGG
Sbjct: 301  ETGMLVVDSVVPGGPAHKLLEPGDVLVRMNGEVITQFLKMETLLDDTVKQTIDLQVERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
             SFTVHLVVQDLHSITPDYFLEV GAVIHPLSYQQARNFRFKC LVYVTEPGYMLFRAGV
Sbjct: 361  ASFTVHLVVQDLHSITPDYFLEVGGAVIHPLSYQQARNFRFKCGLVYVTEPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA
Sbjct: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRHVHQVNN 540
            PPQIYVR+D+TGLWIAKPAFQPHL L+SSPMTN+G+GYMN TDV SDDSSH+RH+H VNN
Sbjct: 481  PPQIYVRNDTTGLWIAKPAFQPHLRLESSPMTNVGEGYMNPTDVLSDDSSHLRHMHPVNN 540

Query: 541  QEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQEPILE 600
             EIIDGVVSMETNFEH SEEARSQDRSD G KKRRVEDDRLTDGNVAD SFHETQE ILE
Sbjct: 541  LEIIDGVVSMETNFEHGSEEARSQDRSDAGTKKRRVEDDRLTDGNVADSSFHETQETILE 600

Query: 601  DETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660
            D TA QTA +RDYQGGTVA +AANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT
Sbjct: 601  DATAMQTANIRDYQGGTVAVVAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQHFFGT 660

Query: 661  GVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720
            GVIIYHS +MGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS
Sbjct: 661  GVIIYHSHNMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAYDPSS 720

Query: 721  LGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQRGDSV 780
            LGSVGA+ VQAAKLLP                                  EPAL+RGDSV
Sbjct: 721  LGSVGAAAVQAAKLLP----------------------------------EPALRRGDSV 780

Query: 781  YLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840
            YLVGLSRSLQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDTDFGSTFSGVLTDE
Sbjct: 781  YLVGLSRSLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDTDFGSTFSGVLTDE 840

Query: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLINGVKRP 900
            HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKI+SG+NGPPLLINGVKRP
Sbjct: 841  HGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKILSGANGPPLLINGVKRP 900

Query: 901  MPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960
            MP+VRILEVELYPTLLSKARSFGLSDEWVQ LVKKDPIRRQVLRVKGCLAGSKAENLLEQ
Sbjct: 901  MPLVRILEVELYPTLLSKARSFGLSDEWVQDLVKKDPIRRQVLRVKGCLAGSKAENLLEQ 960

Query: 961  GDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020
            GDMVLAINKQPITCFYDIENACQELDKN +TDGKLNMTIFRQGHEIDLLVGTDVRDGNGT
Sbjct: 961  GDMVLAINKQPITCFYDIENACQELDKNNSTDGKLNMTIFRQGHEIDLLVGTDVRDGNGT 1020

Query: 1021 TRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080
            TR+INWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL
Sbjct: 1021 TRIINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEVNGKL 1080

Query: 1081 TPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAMWRRV 1140
            TPDLD FVNVTKELEHE+FVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDP+TAMWRRV
Sbjct: 1081 TPDLDTFVNVTKELEHEEFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPDTAMWRRV 1115

Query: 1141 TIKALNPGN 1149
            TIKALN  N
Sbjct: 1141 TIKALNSNN 1115

BLAST of MC08g1774 vs. TAIR 10
Match: AT3G03380.1 (DegP protease 7 )

HSP 1 Score: 1690.2 bits (4376), Expect = 0.0e+00
Identity = 849/1149 (73.89%), Postives = 956/1149 (83.20%), Query Frame = 0

Query: 1    MADPAETLGSESAAIGIDSTAKDELCMEIDPPLRDNLATADDWRKALNKVVPAVVVLRTT 60
            M DP E LGS+ A++  +S  K++LC+EIDPPL +++ATA+DWR+AL KVVPAVVVLRTT
Sbjct: 1    MGDPLERLGSQ-ASMATESVMKEDLCLEIDPPLTESVATAEDWRRALGKVVPAVVVLRTT 60

Query: 61   ACRAFDTESAGASYATGFVVDKRRGIILTNRHVVKPGPVVAEAMFVNREEVPVRPIYRDP 120
            ACRAFDTESAGASYATGF+VDKRRGIILTNRHVVKPGPVVAEAMFVNREE+P+ P+YRDP
Sbjct: 61   ACRAFDTESAGASYATGFIVDKRRGIILTNRHVVKPGPVVAEAMFVNREEIPIYPVYRDP 120

Query: 121  VHDFGFFRYDPGAIQFLNYEEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREA 180
            VHDFGFF YDP A+QFL Y+EIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDR+A
Sbjct: 121  VHDFGFFCYDPSAVQFLTYQEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDRDA 180

Query: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLERV 240
            PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPL+RV
Sbjct: 181  PHYKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLQRV 240

Query: 241  VRALTFLQKGRDSYVNKWEAVSIPRGTLQATFLHKGFDEIRRLGLRSETEQMVRLASPPG 300
            VRAL+FLQK  DS  +K +AV IPRGTLQ TFLHKGFDEIRRLGLRSETEQ+VR ASP G
Sbjct: 241  VRALSFLQKSIDSRTDKPKAVHIPRGTLQMTFLHKGFDEIRRLGLRSETEQVVRHASPTG 300

Query: 301  ETGMLVVDSVVPGGPAHKLLDPGDVLVRMNGEVITQFLKMETLIDDSVNQTIDIQVERGG 360
            ETGMLVVDSVVP GPA K L+PGDVLVR+NG V+TQFL +E L+DD V Q +++++ERGG
Sbjct: 301  ETGMLVVDSVVPSGPADKHLEPGDVLVRVNGTVLTQFLNLENLLDDGVGQILELEIERGG 360

Query: 361  TSFTVHLVVQDLHSITPDYFLEVSGAVIHPLSYQQARNFRFKCSLVYVTEPGYMLFRAGV 420
               +V + VQDLHSITPD+FLEVSGAVIHPLSYQQARNFRF C L YV +PGYMLFRAGV
Sbjct: 361  QPLSVSVSVQDLHSITPDHFLEVSGAVIHPLSYQQARNFRFPCGLAYVADPGYMLFRAGV 420

Query: 421  PRHAIIKKFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRKSVLVTVDHHEWYA 480
            PRHAIIKK A E+IS + DLVSVLSKLSRG RVPLEY+S+TDRHR+KSVLVT+DHHEWYA
Sbjct: 421  PRHAIIKKVANEDISSLGDLVSVLSKLSRGARVPLEYMSHTDRHRKKSVLVTIDHHEWYA 480

Query: 481  PPQIYVRDDSTGLWIAKPAFQPHLHLQSSPMTNLGDGYMNQTDVFSDDSSHIRH----VH 540
            PPQ+Y R+DS+GLW AKPA +P     +S   ++G    N     S D S   H    +H
Sbjct: 481  PPQLYTRNDSSGLWDAKPAIEP-----ASVSPSIG----NNGFPISQDISLCHHDTEPMH 540

Query: 541  QVNNQEIIDGVVSMETNFEHVSEEARSQDRSDVGIKKRRVEDDRLTDGNVADCSFHETQE 600
            +VN + + D    MET     S    SQ+      KK+RV++D  +DG  A+ S + + E
Sbjct: 541  EVNVRGVTDIAAIMET-----SSGDGSQNDFGSEAKKQRVDEDS-SDGIAANGSLYGS-E 600

Query: 601  PILEDETAAQTAILRDYQGGTVAAIAANASFPERIIEPTLVMFEVHVPPSCMLDGVHSQH 660
               +D     T +LRD++G T  A++ANAS  ER IEP LVMFEVHVPPSC LDGVHSQH
Sbjct: 601  FKSDDAMETDTTVLRDFEGAT--ALSANASLAERAIEPALVMFEVHVPPSCSLDGVHSQH 660

Query: 661  FFGTGVIIYHSQSMGLVAVDKNTVAISACDIMLSFAAFPIEIPGEVVFLHPVHNYALVAY 720
            FFGTG+IIYHS +MGL  VDKNTVAISA D+MLSFAAFP+EIPGEVVFLHPVHNYAL+AY
Sbjct: 661  FFGTGIIIYHSSNMGLAVVDKNTVAISASDVMLSFAAFPVEIPGEVVFLHPVHNYALIAY 720

Query: 721  DPSSLGSVGASVVQAAKLLPGKFLLLLLLFIIIIIFCFDYLTLLHKVSLYPSLTEPALQR 780
            +PS++    ASV++AA+LLP                                  EPALQR
Sbjct: 721  NPSAMDPASASVIRAAELLP----------------------------------EPALQR 780

Query: 781  GDSVYLVGLSRSLQATSRKSIVTNPCAALNISSADSPRYRATNMEVIELDTDFGSTFSGV 840
            GDSVYLVGLSR+LQATSRKSIVTNPCAALNI SADSPRYRATNMEVIELDTDFGS+FSG 
Sbjct: 781  GDSVYLVGLSRNLQATSRKSIVTNPCAALNIGSADSPRYRATNMEVIELDTDFGSSFSGA 840

Query: 841  LTDEHGRVQAIWGSFSTQLKFGCSSSEDHQFVRGIPIYTISQVLDKIISGSNGPPLLING 900
            LTDE GR++AIWGSFSTQ+K+  +SSEDHQFVRGIP+Y ISQVL+KII+G NGP LLING
Sbjct: 841  LTDEQGRIRAIWGSFSTQVKYSSTSSEDHQFVRGIPVYAISQVLEKIITGGNGPALLING 900

Query: 901  VKRPMPVVRILEVELYPTLLSKARSFGLSDEWVQALVKKDPIRRQVLRVKGCLAGSKAEN 960
            VKRPMP+VRILEVELYPTLLSKARSFGLSDEW+Q LVKKDP+RRQVLRVKGCLAGSKAEN
Sbjct: 901  VKRPMPLVRILEVELYPTLLSKARSFGLSDEWIQVLVKKDPVRRQVLRVKGCLAGSKAEN 960

Query: 961  LLEQGDMVLAINKQPITCFYDIENACQELDKNYTTDGKLNMTIFRQGHEIDLLVGTDVRD 1020
            LLEQGDMVLA+NK P+TCF DIE ACQ LDK   +D  LN+TI RQG E++L+VGTD RD
Sbjct: 961  LLEQGDMVLAVNKMPVTCFNDIEAACQTLDKGSYSDENLNLTILRQGQELELVVGTDKRD 1020

Query: 1021 GNGTTRVINWCGCIVQDPHPAVRALGFLPEEGHGVYVARWCHGSPVHRYGLYALQWIVEV 1080
            GNGTTRVINWCGC+VQDPHPAVRALGFLPEEGHGVYV RWCHGSP HRYGLYALQWIVEV
Sbjct: 1021 GNGTTRVINWCGCVVQDPHPAVRALGFLPEEGHGVYVTRWCHGSPAHRYGLYALQWIVEV 1080

Query: 1081 NGKLTPDLDAFVNVTKELEHEQFVRVRTVHLNGKPRVLTLKQNLHYWPTWELRFDPETAM 1140
            NGK TPDL+AF + TKELEH QFVR+RTVHLNGKPRVLTLKQ+LHYWPTWELRFDPETA+
Sbjct: 1081 NGKKTPDLNAFADATKELEHGQFVRIRTVHLNGKPRVLTLKQDLHYWPTWELRFDPETAL 1096

Query: 1141 WRRVTIKAL 1146
            WRR  +KAL
Sbjct: 1141 WRRNILKAL 1096

BLAST of MC08g1774 vs. TAIR 10
Match: AT3G03370.1 (BEST Arabidopsis thaliana protein match is: DegP protease 7 (TAIR:AT3G03380.1); Has 55 Blast hits to 55 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 55; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 62.0 bits (149), Expect = 3.6e-09
Identity = 30/40 (75.00%), Postives = 35/40 (87.50%), Query Frame = 0

Query: 428 KFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRK 468
           K A E+IS + DLVSVLSKLSRG RVPLEY+S+TDRHR+K
Sbjct: 43  KVANEDISGLGDLVSVLSKLSRGARVPLEYMSHTDRHRKK 82

BLAST of MC08g1774 vs. TAIR 10
Match: AT3G03370.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 7 growth stages; BEST Arabidopsis thaliana protein match is: DegP protease 7 (TAIR:AT3G03380.1). )

HSP 1 Score: 62.0 bits (149), Expect = 3.6e-09
Identity = 30/40 (75.00%), Postives = 35/40 (87.50%), Query Frame = 0

Query: 428 KFAGEEISRVEDLVSVLSKLSRGTRVPLEYISYTDRHRRK 468
           K A E+IS + DLVSVLSKLSRG RVPLEY+S+TDRHR+K
Sbjct: 43  KVANEDISGLGDLVSVLSKLSRGARVPLEYMSHTDRHRKK 82