MC05g0252 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0252
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DAR GTPase 2, mitochondrial
Location	MC05: 1830723 .. 1834998 (+)
RNA-Seq Expression	MC05g0252
Synteny	MC05g0252

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CGATAGCAACGGCGTCCCCCCTCGGCGCTGTCTCAAAATTACCATGAACTCCGCCGTAGACGATCTCTTCCCCCGACCCGCGAAACAGTACTATATAATCTTCCGAGTCTAAACATCGATTCGAAGTTTGGAGCCAAAAATGGCCGCGGCAACATTCGCGAGAAGAATAGGTACGGCTATTAAGGAAACAGCAATCGGAAACCGGACATCCGGCGGATGGTACGATCCTCACATGGCGGCCGCTTCTCGTGCCGTCGCCGAACGAATTCCACTGGTTGATTTTGTTCTTGAGGTCAGAGATGCTAGGGTATGCTTGCTTCTACTATTGTTTATTCGCTCTCATTGTATGTTGTTTGATTTGGCATCGGGACGCCATGTGTTCGTTGAAATGTCGGTTCCTATTGAGTTCACTTTTTAGTTTCAGTTCTTTTCGGAAACCTGGATTTATTTATTCTTTTGCGTGGTGATTCGCTTCGTTTTCGATAGAAGAATGTAGAACAGTGAAGTTGTATTGTAAATAAGGACTGCGTTATTATTATGAAGGAGAGAAAGAATCACTTAAAATGTTTGTTTATTTCTCTCCGTGTTATTGCTTTTTTTTTTTTTTTCCTTCAGATTCCAGTGTCATCTGAATATGAAATCATGAAAAATCATTCACCATCCTCGAAACGAATCATTGTAATGAACAAGGTGGACCTGGCGGATCGGTCACAGACAGAGGTTCTGAACTTTTCTAACTTCTATGTTCTTCGGCATAGAAATCACTTGTGAGCTTTTACTGTTGGAGTTACTTTCAACTCAAGTCATTCGGGATTTCGCAGGTCAATGTAGCTTATGTGATTCGTTACTGAAGTCTGTCTCGTAGTTTTTTGATTAAATATGACAATGACAAGTTTGTGGGATATTTTGAAAGGATAAGAGCAGAAGATACGCATAGAATATCTATGGGCCAGAATTTATTTTCTCCCCTTGTTATGGTCAATGATCGCACAGGAGTTCCGTAATTTTCCATTTTTACGATTTTCGCACACGGAAGAGTTTTTTACTTCAATTTTTCCCCTTTTGGTTTAAGTTGGATACCTGGCCACCTTCTTTGATCTTGCAATTATCTGTTGATAGAAATACTAATTGTTTTTCTTATATGTAAAAAAGGAATTTGATTATATGGAATCGGATTAGTACTTTAGCTATCAATTTACATTAATCCATCAGTACATATAACATTTTGTGAATTTATTTTTGCTTTTACAATTTAAACCATATTCGAAATGATTTTTCAGTCTGTTTCCATTGTTTCACACTTCTATACTGCATTCTGTTAACAGGTTTGGACAAAGTATTTTGAAGATCACAATTGCGTTTCTTATGGCGTTAATGCCCATAATAAGGATAACATTAGGGAGGTAAAGAACTACGCGATGAACTAAAGATGCGATGTTTATGTGTTCTTTTTTTATTTTTGCTATCTTGTTTGATCTACTCTTATTCCTTTTTCTTTGGCTTAGGAATGCTGGTGATAGTAATTGTTAGATGTCTTGTTGATATATATGTTTATTCTCCCAGCTTGTTTCAAGGAAAATTTTAAGTGTAATGTCAATTAAATGTAATTTATATACAAAAACTTCCCCACATTTCAATTGTCAGTTGATACTTTCTTATCATGGGACAATCCAATAGTTGTTTATTCTTTTGAGCTGGCCTTAGTTGTTCATGTTCTTGATGTAATAGTAATTGGTGGATGGTTCACAGTTTCTGAACTTTCTACAAGCTAGAGTGAGAGAACTGAAGAAGAGTGGTCATTCTAGTCATGCCACAACAATGATGCTGGTTGGAATTCCAAATGTTGGTAAGTCGGCCCTTGCCAACTCTCTGCACCAAATTGGGAGAATTAGTGCTGCAGGTACATCTCTTAAATCCCACAGAATTTGAATTTTCTAGAGAAGTTGATACTGGAATTATTTGGATGAATGATGATTGTTATTTATATGATAACAAAAAATTGCTACATGTGACATTTTGAAGAACTGCTCACCTCACAGAAAAAGGGAAGTTGAAGCATGCTGTGGTGAGTCCAGAGCCTGGTGAAACAAAAAATATCAGCAGCTTGAAGGTCTGTCGCTTTTCTCCTCAACAATGTATTAGGTGGTGAACTTTTCTTACTGATTTGAGGTTACTGCGTTACCTGCTTAGGTGTTTATCAAATTCTTATATTGGTATATTTTGAATTGAACAAGGCTTAAACAAGAGTAGAGGAAACTGAACCACAATAAATCTGTTATTTGATTTTATAATAGTGGGCAGCAAAGAAAAGATCTCTCATTAAGCTATGGGCACGAATATCATGTATCCTTTTTGGAAGCAAAGATAATATGTCTACTTTTGCTTTGTATAAGCTTTTGAATTTTATCTTTAAGTTTCTTTGTATTACAGATAGCTAGTCATCCCAATATTTATGTGTTGGACACCCCAGGCATTTTGCCTCCCAAGATTCTCAATATCGAGGTCTGTTCCAAGCTTGCTTTAACAGGTACAATATTATGTAGCCAACTTATCCATCTTCCTTGAGTAAACTATTTAAGTTGGTGAATTAGATACTGAAACACGCATACGAAGTGATAAATTTACTTGTGTGTGTGTGTGTGTGTTTTTTTTTCTCCTTTCTCCTGGTTGTAGCACTAGTGCTATTTCAGAGACAGTGGAAATGATCAAGAATCTTGAAACTATCAACTTTTTTGTTTTGCCTCCAAACTGTAGGAGCTATTAGAGACATCTTAGTTGGGGAAAGAGTAATTGTACAGTACCTTTTAACTATTCTCAACTCAAGTGATAAATATAAGAAGTGGGCAAGTTTGTCTGCCACCAACTGTGACAGCTCGTTGGAGTGTTCAACTTGCTCAAATTCAGAGAGTCAGAAAAGGGGGTACCCCTCTGATCACACGCAGGTATGCATTATATGGTTATTTTTTTCTTCCCAAAAGCATTATGTATGTATACTGAACAGTGAACCCAATTCATGCAGGTTTATTTTGCAATCAAAGTCTTTGTAGATGTCTTTAGGTTTCACATTATTCTCCATCTATTATTAGAGAGGGGACACATGGTTGATTCCTTACTAAAATAGTTTATTAATATATAATTTTTGAGGACACATGGTCGATTCCTTACTAAAATATTTATTTATATATAATTTTTGAGGTAATCTCTCACATTGGTGATATGCTTACTAACCAAAGTAACTGGTAAGTGGAAAAGTTAAGTGCTTTTTCAAATGTTACCTCGGGTACGCGCGATACTTTCTAGTTCCTTGCCAATGCAAGATTGGTCCAAATACAGTAGTTGATCACTCTTCTTGTCTGTTATTATCTTTGGATGTGTTGGTTTGTAGTTTACACTTTACAACTCAGCCTTTGGACGTAAAACTGACGCTGTGAGAGACTTGAGAATGCAGTGCTCCTTATATTTTCAGCGACACTAGGCTTAAGAACATACTACATTATATTCATCTCAAGTTTATGGAACTGTTGTTAGTAAAAGACCACTGGGCTGAAAATGATAGATTTGGTTGTGTTTGCAGGACTTTATTGTAAACGATGTTCGTAGGATTCTCTTCGAAACTATATCATCTTTTGATGGTAAAACGGAGGATGAAAAGGACATGGGAAACTTTATTGAGGTACAATTGCTTGCTCTGCACAAAGCTTTGCACGTTCCTATGGATTCTAGCAATGATGCTTCTACCAAGGTTGCTTCTAAACTTTTGAATCTTTATCGTACCGGGAGGCTTGGTCGTTACACACTTGATTCTGTACCTGTTAATTCCTAGTTTACAAAACCTTGTAAGATTATGCTGCTTGATATAGTTTCTTTTCAGCCTCCACATTCATTACATTACACAATCGGCGGCACATTCTCTGTACATAAAATTGGTAAAAAGATGGAACGGACAGGTAAAAAACACTAAAAAACTTTTCTTGTCATGGAAGTTTCGAGGAAACGTCCTGAAATTCCATCAACTTTTTTTTTTTTTTTCCGAGTTTGATATTGTTGAAAATTCTGATCTTGGCTTGATAGAGCTCTCATGCTATGCTAGGGAGACAAATTTCTCATTGAATAAAATTCTTTTTTTCTTTGAAGTATAAATGAAAGATAATCATCAGATAGGTTCGAAGAGCAATTCTCAAAGTCACAACAAAAGCCTTAAACATATTGACTTGAGCTAGTTTGATGTTAATCTTTTTATTACATATATTCCTCTTGGATTCCTCTTGGAAAGTAG

mRNA sequence

CGATAGCAACGGCGTCCCCCCTCGGCGCTGTCTCAAAATTACCATGAACTCCGCCGTAGACGATCTCTTCCCCCGACCCGCGAAACAGTACTATATAATCTTCCGAGTCTAAACATCGATTCGAAGTTTGGAGCCAAAAATGGCCGCGGCAACATTCGCGAGAAGAATAGGTACGGCTATTAAGGAAACAGCAATCGGAAACCGGACATCCGGCGGATGGTACGATCCTCACATGGCGGCCGCTTCTCGTGCCGTCGCCGAACGAATTCCACTGGTTGATTTTGTTCTTGAGGTCAGAGATGCTAGGATTCCAGTGTCATCTGAATATGAAATCATGAAAAATCATTCACCATCCTCGAAACGAATCATTGTAATGAACAAGGTGGACCTGGCGGATCGGTCACAGACAGAGGTTTGGACAAAGTATTTTGAAGATCACAATTGCGTTTCTTATGGCGTTAATGCCCATAATAAGGATAACATTAGGGAGTTTCTGAACTTTCTACAAGCTAGAGTGAGAGAACTGAAGAAGAGTGGTCATTCTAGTCATGCCACAACAATGATGCTGGTTGGAATTCCAAATGTTGGTAAGTCGGCCCTTGCCAACTCTCTGCACCAAATTGGGAGAATTAGTGCTGCAGAAAAAGGGAAGTTGAAGCATGCTGTGGTGAGTCCAGAGCCTGGTGAAACAAAAAATATCAGCAGCTTGAAGATAGCTAGTCATCCCAATATTTATGTGTTGGACACCCCAGGCATTTTGCCTCCCAAGATTCTCAATATCGAGGTCTGTTCCAAGCTTGCTTTAACAGGAGCTATTAGAGACATCTTAGTTGGGGAAAGAGTAATTGTACAGTACCTTTTAACTATTCTCAACTCAAGTGATAAATATAAGAAGTGGGCAAGTTTGTCTGCCACCAACTGTGACAGCTCGTTGGAGTGTTCAACTTGCTCAAATTCAGAGAGTCAGAAAAGGGGGTACCCCTCTGATCACACGCAGGACTTTATTGTAAACGATGTTCGTAGGATTCTCTTCGAAACTATATCATCTTTTGATGGTAAAACGGAGGATGAAAAGGACATGGGAAACTTTATTGAGGTACAATTGCTTGCTCTGCACAAAGCTTTGCACGTTCCTATGGATTCTAGCAATGATGCTTCTACCAAGGTTGCTTCTAAACTTTTGAATCTTTATCGTACCGGGAGGCTTGGTCGTTACACACTTGATTCTGTACCTGTTAATTCCTAGTTTACAAAACCTTGTAAGATTATGCTGCTTGATATAGTTTCTTTTCAGCCTCCACATTCATTACATTACACAATCGGCGGCACATTCTCTGTACATAAAATTGGTAAAAAGATGGAACGGACAGGTAAAAAACACTAAAAAACTTTTCTTGTCATGGAAGTTTCGAGGAAACGTCCTGAAATTCCATCAACTTTTTTTTTTTTTTTCCGAGTTTGATATTGTTGAAAATTCTGATCTTGGCTTGATAGAGCTCTCATGCTATGCTAGGGAGACAAATTTCTCATTGAATAAAATTCTTTTTTTCTTTGAAGTATAAATGAAAGATAATCATCAGATAGGTTCGAAGAGCAATTCTCAAAGTCACAACAAAAGCCTTAAACATATTGACTTGAGCTAGTTTGATGTTAATCTTTTTATTACATATATTCCTCTTGGATTCCTCTTGGAAAGTAG

Coding sequence (CDS)

ATGGCCGCGGCAACATTCGCGAGAAGAATAGGTACGGCTATTAAGGAAACAGCAATCGGAAACCGGACATCCGGCGGATGGTACGATCCTCACATGGCGGCCGCTTCTCGTGCCGTCGCCGAACGAATTCCACTGGTTGATTTTGTTCTTGAGGTCAGAGATGCTAGGATTCCAGTGTCATCTGAATATGAAATCATGAAAAATCATTCACCATCCTCGAAACGAATCATTGTAATGAACAAGGTGGACCTGGCGGATCGGTCACAGACAGAGGTTTGGACAAAGTATTTTGAAGATCACAATTGCGTTTCTTATGGCGTTAATGCCCATAATAAGGATAACATTAGGGAGTTTCTGAACTTTCTACAAGCTAGAGTGAGAGAACTGAAGAAGAGTGGTCATTCTAGTCATGCCACAACAATGATGCTGGTTGGAATTCCAAATGTTGGTAAGTCGGCCCTTGCCAACTCTCTGCACCAAATTGGGAGAATTAGTGCTGCAGAAAAAGGGAAGTTGAAGCATGCTGTGGTGAGTCCAGAGCCTGGTGAAACAAAAAATATCAGCAGCTTGAAGATAGCTAGTCATCCCAATATTTATGTGTTGGACACCCCAGGCATTTTGCCTCCCAAGATTCTCAATATCGAGGTCTGTTCCAAGCTTGCTTTAACAGGAGCTATTAGAGACATCTTAGTTGGGGAAAGAGTAATTGTACAGTACCTTTTAACTATTCTCAACTCAAGTGATAAATATAAGAAGTGGGCAAGTTTGTCTGCCACCAACTGTGACAGCTCGTTGGAGTGTTCAACTTGCTCAAATTCAGAGAGTCAGAAAAGGGGGTACCCCTCTGATCACACGCAGGACTTTATTGTAAACGATGTTCGTAGGATTCTCTTCGAAACTATATCATCTTTTGATGGTAAAACGGAGGATGAAAAGGACATGGGAAACTTTATTGAGGTACAATTGCTTGCTCTGCACAAAGCTTTGCACGTTCCTATGGATTCTAGCAATGATGCTTCTACCAAGGTTGCTTCTAAACTTTTGAATCTTTATCGTACCGGGAGGCTTGGTCGTTACACACTTGATTCTGTACCTGTTAATTCCTAG

Protein sequence

MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLNFLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYLLTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFETISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYTLDSVPVNS

Homology

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BLAST of MC05g0252 vs. ExPASy Swiss-Prot
Match: O82497 (DAR GTPase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DGP2 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 8.6e-119
Identity = 224/361 (62.05%), Postives = 273/361 (75.62%), Query Frame = 0


Query: 7   ARRIGTA-IKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEI 66
           AR IG A IK +   NR    WY PHMAAA RA++ERIPLVDFVLE+RDARIP+SSEYE+
Sbjct: 10  AREIGDAVIKASRNPNRR---WYGPHMAAAVRAISERIPLVDFVLEIRDARIPLSSEYEL 69

Query: 67  MKNHSP-SSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLNFLQA 126
           ++  SP  SKRIIV+NK++LAD  + +    YFE+ N +SY VN+HNKD +++ LNFLQ+
Sbjct: 70  LRKFSPLPSKRIIVLNKMELADPLELKKCIDYFEERNYLSYAVNSHNKDCVKQLLNFLQS 129

Query: 127 RVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGET 186
           +VREL K+GHS H TTMML+GIPNVGKSAL+NSLH IGRISAAEKGKLKH  VS +PG+T
Sbjct: 130 QVRELHKAGHSGHTTTMMLLGIPNVGKSALSNSLHHIGRISAAEKGKLKHTTVSSQPGDT 189

Query: 187 KNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYLLTIL 246
           K+I SLKI SHPN+YVLDTPGI PP + + E+C+KLALTGAI D +VGE  + +  LTIL
Sbjct: 190 KDIMSLKIGSHPNVYVLDTPGIFPPNLYDAEICAKLALTGAIPDDIVGELKLARLFLTIL 249

Query: 247 NSSDKYKKWASL-SATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFETISS 306
           NSS +YKKWA L  + +   SL   +  +    KR Y +DHTQDFIV DVRR+L+ETIS+
Sbjct: 250 NSSHEYKKWAKLCKSQDLTESLSDESSKSDAKHKRQYATDHTQDFIVYDVRRVLYETISA 309

Query: 307 FDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYTLDS 365
           FDG  EDE  MGN IE Q  AL   L VP ++S  A  +VASK+LNLYRTGRLG YTL+ 
Sbjct: 310 FDGNLEDEISMGNLIETQFAALRSVLRVPEEASEFADLRVASKILNLYRTGRLGHYTLEH 367

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BLAST of MC05g0252 vs. ExPASy Swiss-Prot
Match: Q8L607 (Short integuments 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SIN2 PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.7e-63
Identity = 140/348 (40.23%), Postives = 205/348 (58.91%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEIMKNHSPSSKRIIVMNKVDLAD 86
           W+  HMAAA+RA+  R+ L D V+EVRDARIP+SS  E +++   + +RII +NK DLA+
Sbjct: 28  WFPGHMAAATRAIRNRLKLSDLVIEVRDARIPLSSANEDLQSQMSAKRRIIALNKKDLAN 87

Query: 87  RSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLNFLQARVRELKKSGHSSHATTMMLVGI 146
            +    WT++FE        +NAH++ ++ + L+ ++ +++E+           +M+VG+
Sbjct: 88  PNVLNKWTRHFESSKQDCIAINAHSRSSVMKLLDLVELKLKEVIA---REPTLLVMVVGV 147

Query: 147 PNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGETKNISSLKIASHPNIYVLDTPGI 206
           PNVGKSAL NS+HQI       + +LK A V P PG T++I+  KIA  P+IYVLD+PG+
Sbjct: 148 PNVGKSALINSIHQIAAARFPVQERLKRATVGPLPGVTQDIAGFKIAHRPSIYVLDSPGV 207

Query: 207 LPPKILNIEVCSKLALTGAIRDILVGERVIVQYLLTILNSSDKYKKWASL-SATNCDSSL 266
           L P I +IE   KLAL+G+++D +VGE  I QY L ILN       W  L    N     
Sbjct: 208 LVPSIPDIETGLKLALSGSVKDSVVGEERIAQYFLAILNIRGTPLHWKYLVEGINEGPHA 267

Query: 267 EC-----STCSNSESQKRGYPSDHTQDFI---VNDVRRILFETISSFDGKTEDEKDMGNF 326
           +C         +   Q+   P      ++   +++V+R L+ T+S FDG TEDE D+   
Sbjct: 268 DCIDKPSYNLKDLRHQRTKQPDSSALHYVGDMISEVQRSLYITLSEFDGDTEDENDLECL 327

Query: 327 IEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYTLDSVP 366
           IE Q   L KAL +P  +S +A   V+ K L L+RTGRLG + LD VP
Sbjct: 328 IEQQFEVLQKALKIPHKAS-EARLMVSKKFLTLFRTGRLGPFILDDVP 371

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BLAST of MC05g0252 vs. ExPASy Swiss-Prot
Match: Q9BT17 (Mitochondrial ribosome-associated GTPase 1 OS=Homo sapiens OX=9606 GN=MTG1 PE=1 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 1.7e-26
Identity = 77/231 (33.33%), Postives = 123/231 (53.25%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEIMKNHSPSSKRIIVMNKVDLAD 86
           W+  HMA   + +   + LVD ++EV DARIP+S    + +        ++V+NK+DLAD
Sbjct: 30  WFPGHMAKGLKKMQSSLKLVDCIIEVHDARIPLSGRNPLFQETLGLKPHLLVLNKMDLAD 89

Query: 87  RSQTEVWTKYFEDHNCVS-YGVNAHNKDNIREFLNFLQARVRELKKSGHSSHAT-----T 146
            ++ +   ++ E     +    N    +N+++ +      V EL    H  H        
Sbjct: 90  LTEQQKIMQHLEGEGLKNVIFTNCVKDENVKQII----PMVTELIGRSHRYHRKENLEYC 149

Query: 147 MMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGETKNI-SSLKIASHPNIY 206
           +M++G+PNVGKS+L NSL    R     KGK     V  EPG T+ + S ++++  P ++
Sbjct: 150 IMVIGVPNVGKSSLINSL----RRQHLRKGKATR--VGGEPGITRAVMSKIQVSERPLMF 209

Query: 207 VLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYLLTILNSSDKY 251
           +LDTPG+L P+I ++E   KLAL G + D LVGE  +  YLL  LN   ++
Sbjct: 210 LLDTPGVLAPRIESVETGLKLALCGTVLDHLVGEETMADYLLYTLNKHQRF 250

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BLAST of MC05g0252 vs. ExPASy Swiss-Prot
Match: A9JTX2 (Mitochondrial ribosome-associated GTPase 1 OS=Xenopus tropicalis OX=8364 GN=mtg1 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 5.0e-26
Identity = 82/248 (33.06%), Postives = 129/248 (52.02%), Query Frame = 0


Query: 15  KETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEIMKNHSPSSK 74
           K    G R    W+  HMA   + +  ++  +D ++EV DARIP+S    I ++      
Sbjct: 9   KSFDFGEREVAHWFPGHMAKGLKQMKTKLKNLDCIVEVHDARIPLSGRNPIFQDSLGMKP 68

Query: 75  RIIVMNKVDLADRSQTE----------VWTKYFEDHNCVSYGVNAHNKDNIREFLNFLQA 134
            ++++NK+DLAD +Q +          V    F D  CV      H    I E +   Q 
Sbjct: 69  HLLILNKMDLADLTQKKRILAQLKQQGVGNVIFTD--CVKDQNIKHVVPVISELVGCSQR 128

Query: 135 RVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGET 194
             RE       +  T +M++G+PNVGKS+L N+L ++       KGK     V  EPG T
Sbjct: 129 FHRE------ENTETCIMVIGVPNVGKSSLINALRRM----HLRKGKASR--VGAEPGIT 188

Query: 195 KNI-SSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYLLTI 252
           +++ + ++++  P I++ DTPG+L P+I ++E   KLAL G I D LVGE ++  YLL I
Sbjct: 189 RSVLTKIQVSESPLIFLFDTPGVLSPRIESVETGMKLALCGTILDHLVGEDIMADYLLYI 242

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BLAST of MC05g0252 vs. ExPASy Swiss-Prot
Match: E3TDS3 (Mitochondrial ribosome-associated GTPase 1 OS=Ictalurus punctatus OX=7998 GN=mtg1 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 6.6e-26
Identity = 78/236 (33.05%), Postives = 122/236 (51.69%), Query Frame = 0


Query: 20  GNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEIMKNHSPSSKRIIVM 79
           G R    W+  HMA   + +      VD V+E+ DARIP S    + + +      ++++
Sbjct: 21  GERQVAHWFPGHMAKGLKQMRASPRKVDCVIEIHDARIPFSGRNPLFQENLDVRPHLLIL 80

Query: 80  NKVDLADRSQTEVWTKYFED---HNCVSYGVNAHNKDNIREFLNFLQARVRELKK-SGHS 139
           NK+DLAD S      K  E     N +          ++++ +  +   +    +     
Sbjct: 81  NKMDLADTSDKMSILKQLERDGVKNVLLTDCLKQRDTSVKKIIPLVTDLIENAPRFHREE 140

Query: 140 SHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGETKNI-SSLKIAS 199
           + +  +M++G+PNVGKS+L N++    R +  +KGK     V  EPG TK + + +++  
Sbjct: 141 NRSYCLMVIGVPNVGKSSLINAI----RRTNLKKGKASR--VGGEPGITKAVLTKIQVCE 200

Query: 200 HPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYLLTILNSSDKY 251
            P I++LDTPG+LPPKI NIE   KLAL G I D LVGE +I  YLL  LN   ++
Sbjct: 201 RPVIHLLDTPGVLPPKIENIETGMKLALCGTILDHLVGEDIIADYLLFSLNRPQRF 250

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BLAST of MC05g0252 vs. NCBI nr
Match: XP_022146907.1 (DAR GTPase 2, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 721 bits (1862), Expect = 4.15e-262
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS
Sbjct: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
           SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN
Sbjct: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET
Sbjct: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT
Sbjct: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360

Query: 361 LDSVPVNS 368
           LDSVPVNS
Sbjct: 361 LDSVPVNS 368

[Close]

BLAST of MC05g0252 vs. NCBI nr
Match: XP_022146908.1 (DAR GTPase 2, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 638 bits (1645), Expect = 1.32e-229
Identity = 333/368 (90.49%), Postives = 333/368 (90.49%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDAR    
Sbjct: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDAR---- 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
                                          VWTKYFEDHNCVSYGVNAHNKDNIREFLN
Sbjct: 61  -------------------------------VWTKYFEDHNCVSYGVNAHNKDNIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET
Sbjct: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT
Sbjct: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 333

Query: 361 LDSVPVNS 368
           LDSVPVNS
Sbjct: 361 LDSVPVNS 333

[Close]

BLAST of MC05g0252 vs. NCBI nr
Match: XP_023546448.1 (DAR GTPase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615 bits (1586), Expect = 4.37e-220
Identity = 309/368 (83.97%), Postives = 337/368 (91.58%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MAAAT  R+IG+AI E AIG+R S GWYDPHMAAASRAV ERIPLVDFVLEVRDAR+P +
Sbjct: 1   MAAATLMRKIGSAINEAAIGSRISSGWYDPHMAAASRAVVERIPLVDFVLEVRDARVPKT 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
           SEYE+MKN+ PSSKRII++NK DLADRSQTE WT++FEDHNC+SYGVN+HNK+NIREFL 
Sbjct: 61  SEYELMKNYPPSSKRIIILNKTDLADRSQTEAWTRFFEDHNCISYGVNSHNKENIREFLT 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLK+A+VSP+
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKNAIVSPQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGE+V+VQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGEQVVVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LT++NSSDKYKKW +LSAT  DS LE STCS+ E QKRGYPSDHTQD IVNDVRRILFET
Sbjct: 241 LTLINSSDKYKKWGNLSAT--DSLLERSTCSSLEKQKRGYPSDHTQDIIVNDVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           IS+FDG  EDEK MGN IE QLLALHKALHVPM   ND+  KVASKLLNLYRTGRLGRYT
Sbjct: 301 ISTFDGNLEDEKGMGNLIETQLLALHKALHVPMGFDNDSPIKVASKLLNLYRTGRLGRYT 360

Query: 361 LDSVPVNS 368
           +DS+P N+
Sbjct: 361 IDSLPNNT 366

[Close]

BLAST of MC05g0252 vs. NCBI nr
Match: XP_038884377.1 (DAR GTPase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 612 bits (1578), Expect = 7.23e-219
Identity = 316/365 (86.58%), Postives = 331/365 (90.68%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MA AT  R+IGTAIKE A G+R S GWYDPHMAAASRAVAERIPL DFVLEVRDAR+P+S
Sbjct: 1   MAGATLMRKIGTAIKEAATGSRISSGWYDPHMAAASRAVAERIPLADFVLEVRDARVPMS 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
           SEYEIMKN+ PSSKRIIV+NK DLADRSQTEVWT+YFEDHNC+SYGVN+HNK+NIREFLN
Sbjct: 61  SEYEIMKNYPPSSKRIIVLNKADLADRSQTEVWTRYFEDHNCISYGVNSHNKENIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKH +VS +
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHTIVSSQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGI PPKI NIEVCSKLALTGAIRDILVGE VIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LTI+NSSDKYKKWA+LSAT  DSSLECS  SN E QKR YPSDHTQD IVN VRRILFET
Sbjct: 241 LTIVNSSDKYKKWANLSAT--DSSLECSN-SNLEKQKRRYPSDHTQDIIVNGVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           ISSFDG  EDEKDMG  IE QL ALHKALHVPMD  NDAS KVASKLLNLYRTGRLG YT
Sbjct: 301 ISSFDGNLEDEKDMGILIETQLHALHKALHVPMDLCNDASIKVASKLLNLYRTGRLGCYT 360

Query: 361 LDSVP 365
           LDS+P
Sbjct: 361 LDSLP 362

[Close]

BLAST of MC05g0252 vs. NCBI nr
Match: XP_022962198.1 (DAR GTPase 2, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 610 bits (1574), Expect = 2.94e-218
Identity = 306/368 (83.15%), Postives = 335/368 (91.03%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MAAAT  R+IG+AI E A+G+R S GWYDPHMAAASRAV ERIPLVDFVLEVRDARIP +
Sbjct: 1   MAAATLMRKIGSAINEAALGSRISSGWYDPHMAAASRAVVERIPLVDFVLEVRDARIPKT 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
           SEYE+MKN+  SSKRII++NK DLADRSQTE WT++FEDHNC+SYGVN+HNK+NIREFL 
Sbjct: 61  SEYELMKNYPLSSKRIIILNKTDLADRSQTEAWTRFFEDHNCISYGVNSHNKENIREFLT 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLK+A+VSP+
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKNAIVSPQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGI PPKILNIEVCSKLALTGAIRDILVGE+++VQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKILNIEVCSKLALTGAIRDILVGEQLLVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LT++NSSDKYKKW +LSAT  DS LECSTC + E QKRGYPSDHTQD IVNDVRRILFET
Sbjct: 241 LTLINSSDKYKKWGNLSAT--DSLLECSTCPSLEKQKRGYPSDHTQDIIVNDVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           IS+FDG  EDEK MGN IE QLLALHKALHVPM   ND+  KVASKLLNLYRTGRLGRYT
Sbjct: 301 ISTFDGNLEDEKGMGNLIETQLLALHKALHVPMGFDNDSPIKVASKLLNLYRTGRLGRYT 360

Query: 361 LDSVPVNS 368
           +DS+P N+
Sbjct: 361 IDSLPNNT 366

[Close]

BLAST of MC05g0252 vs. ExPASy TrEMBL
Match: A0A6J1D0W3 (DAR GTPase 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015994 PE=4 SV=1)

HSP 1 Score: 721 bits (1862), Expect = 2.01e-262
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS
Sbjct: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
           SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN
Sbjct: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET
Sbjct: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT
Sbjct: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360

Query: 361 LDSVPVNS 368
           LDSVPVNS
Sbjct: 361 LDSVPVNS 368

[Close]

BLAST of MC05g0252 vs. ExPASy TrEMBL
Match: A0A6J1CZT5 (DAR GTPase 2, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015994 PE=4 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 6.38e-230
Identity = 333/368 (90.49%), Postives = 333/368 (90.49%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDAR    
Sbjct: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDAR---- 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
                                          VWTKYFEDHNCVSYGVNAHNKDNIREFLN
Sbjct: 61  -------------------------------VWTKYFEDHNCVSYGVNAHNKDNIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET
Sbjct: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT
Sbjct: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 333

Query: 361 LDSVPVNS 368
           LDSVPVNS
Sbjct: 361 LDSVPVNS 333

[Close]

BLAST of MC05g0252 vs. ExPASy TrEMBL
Match: A0A6J1HE44 (DAR GTPase 2, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462723 PE=4 SV=1)

HSP 1 Score: 610 bits (1574), Expect = 1.42e-218
Identity = 306/368 (83.15%), Postives = 335/368 (91.03%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MAAAT  R+IG+AI E A+G+R S GWYDPHMAAASRAV ERIPLVDFVLEVRDARIP +
Sbjct: 1   MAAATLMRKIGSAINEAALGSRISSGWYDPHMAAASRAVVERIPLVDFVLEVRDARIPKT 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
           SEYE+MKN+  SSKRII++NK DLADRSQTE WT++FEDHNC+SYGVN+HNK+NIREFL 
Sbjct: 61  SEYELMKNYPLSSKRIIILNKTDLADRSQTEAWTRFFEDHNCISYGVNSHNKENIREFLT 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLK+A+VSP+
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKNAIVSPQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGI PPKILNIEVCSKLALTGAIRDILVGE+++VQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKILNIEVCSKLALTGAIRDILVGEQLLVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LT++NSSDKYKKW +LSAT  DS LECSTC + E QKRGYPSDHTQD IVNDVRRILFET
Sbjct: 241 LTLINSSDKYKKWGNLSAT--DSLLECSTCPSLEKQKRGYPSDHTQDIIVNDVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           IS+FDG  EDEK MGN IE QLLALHKALHVPM   ND+  KVASKLLNLYRTGRLGRYT
Sbjct: 301 ISTFDGNLEDEKGMGNLIETQLLALHKALHVPMGFDNDSPIKVASKLLNLYRTGRLGRYT 360

Query: 361 LDSVPVNS 368
           +DS+P N+
Sbjct: 361 IDSLPNNT 366

[Close]

BLAST of MC05g0252 vs. ExPASy TrEMBL
Match: A0A6J1KB62 (DAR GTPase 2, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492297 PE=4 SV=1)

HSP 1 Score: 609 bits (1570), Expect = 6.01e-218
Identity = 306/368 (83.15%), Postives = 335/368 (91.03%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           M AAT  R+IG+AI E AIG+R + GWYDPHMAAASRAV ERIPLVDFVLEVRDARIP +
Sbjct: 1   MDAATLMRKIGSAINEAAIGSRITSGWYDPHMAAASRAVVERIPLVDFVLEVRDARIPKT 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
           SEYE+MKN+ PSSKRII++NK DLADRSQTE WT+ FEDHNC+SYGVN+HNK+NIREFL 
Sbjct: 61  SEYELMKNYLPSSKRIIILNKTDLADRSQTEAWTRLFEDHNCISYGVNSHNKENIREFLT 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLK+A+VSP+
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKNAIVSPQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGI PPKILNIEVCSKLALTGAIRDILVGE+V+VQ+L
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKILNIEVCSKLALTGAIRDILVGEQVLVQFL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LT++NSSDKYKKW +LSAT  DS LECSTCS+ E +KRGYPSDHTQD IVNDVRRILFET
Sbjct: 241 LTLINSSDKYKKWGNLSAT--DSLLECSTCSSLEKRKRGYPSDHTQDIIVNDVRRILFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
           IS+FDG  ED+K MGN IE QLLALHKALHVPM   ND+  KVASKLLNLYRTGRLGRYT
Sbjct: 301 ISTFDGNLEDKKGMGNLIETQLLALHKALHVPMGFDNDSPIKVASKLLNLYRTGRLGRYT 360

Query: 361 LDSVPVNS 368
           LDS+P N+
Sbjct: 361 LDSLPNNT 366

[Close]

BLAST of MC05g0252 vs. ExPASy TrEMBL
Match: A0A1S3BA35 (DAR GTPase 2, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487836 PE=4 SV=1)

HSP 1 Score: 594 bits (1532), Expect = 3.55e-212
Identity = 306/366 (83.61%), Postives = 327/366 (89.34%), Query Frame = 0


Query: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60
           MAAAT  R+IGTAI E A GNR S GWYD HMAAASRAVAERIPLVDFVLEVRDARIP+S
Sbjct: 1   MAAATLMRKIGTAINELAAGNRISSGWYDAHMAAASRAVAERIPLVDFVLEVRDARIPMS 60

Query: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120
           SEYE+MKNH PSSKRIIV+NK DLAD+ QTEVWT+YFEDHNC+SY VN+HNK+NIREFLN
Sbjct: 61  SEYEMMKNHPPSSKRIIVLNKTDLADQLQTEVWTRYFEDHNCISYCVNSHNKENIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180
           FLQ+RVRELK+SGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVS +
Sbjct: 121 FLQSRVRELKRSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSSQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240
           PGETKNISSLKIASHPN+YVLDTPGI PPKI NIEVCSKLALTGAIRDILVGE VIVQYL
Sbjct: 181 PGETKNISSLKIASHPNVYVLDTPGIFPPKIDNIEVCSKLALTGAIRDILVGEHVIVQYL 240

Query: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300
           LTI+NSS KYKKWA+LSA    SSLECST S+ E QKR YPSDHTQD IVN+VRR LFET
Sbjct: 241 LTIVNSSVKYKKWANLSA----SSLECSTPSSLEKQKRRYPSDHTQDIIVNEVRRSLFET 300

Query: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360
            SSFDG  EDEKDMG+ IE QL  LHKALHVPMD  N  + KVASKLLNLYRTGRLGRYT
Sbjct: 301 RSSFDGNLEDEKDMGSLIETQLHTLHKALHVPMDFCNSTTIKVASKLLNLYRTGRLGRYT 360

Query: 361 LDSVPV 366
           LDS+P+
Sbjct: 361 LDSLPL 362

[Close]

BLAST of MC05g0252 vs. TAIR 10
Match: AT4G10650.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 6.1e-120
Identity = 224/361 (62.05%), Postives = 273/361 (75.62%), Query Frame = 0


Query: 7   ARRIGTA-IKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEI 66
           AR IG A IK +   NR    WY PHMAAA RA++ERIPLVDFVLE+RDARIP+SSEYE+
Sbjct: 10  AREIGDAVIKASRNPNRR---WYGPHMAAAVRAISERIPLVDFVLEIRDARIPLSSEYEL 69

Query: 67  MKNHSP-SSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLNFLQA 126
           ++  SP  SKRIIV+NK++LAD  + +    YFE+ N +SY VN+HNKD +++ LNFLQ+
Sbjct: 70  LRKFSPLPSKRIIVLNKMELADPLELKKCIDYFEERNYLSYAVNSHNKDCVKQLLNFLQS 129

Query: 127 RVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGET 186
           +VREL K+GHS H TTMML+GIPNVGKSAL+NSLH IGRISAAEKGKLKH  VS +PG+T
Sbjct: 130 QVRELHKAGHSGHTTTMMLLGIPNVGKSALSNSLHHIGRISAAEKGKLKHTTVSSQPGDT 189

Query: 187 KNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYLLTIL 246
           K+I SLKI SHPN+YVLDTPGI PP + + E+C+KLALTGAI D +VGE  + +  LTIL
Sbjct: 190 KDIMSLKIGSHPNVYVLDTPGIFPPNLYDAEICAKLALTGAIPDDIVGELKLARLFLTIL 249

Query: 247 NSSDKYKKWASL-SATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFETISS 306
           NSS +YKKWA L  + +   SL   +  +    KR Y +DHTQDFIV DVRR+L+ETIS+
Sbjct: 250 NSSHEYKKWAKLCKSQDLTESLSDESSKSDAKHKRQYATDHTQDFIVYDVRRVLYETISA 309

Query: 307 FDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYTLDS 365
           FDG  EDE  MGN IE Q  AL   L VP ++S  A  +VASK+LNLYRTGRLG YTL+ 
Sbjct: 310 FDGNLEDEISMGNLIETQFAALRSVLRVPEEASEFADLRVASKILNLYRTGRLGHYTLEH 367

[Close]

BLAST of MC05g0252 vs. TAIR 10
Match: AT2G41670.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 4.8e-64
Identity = 140/348 (40.23%), Postives = 205/348 (58.91%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEIMKNHSPSSKRIIVMNKVDLAD 86
           W+  HMAAA+RA+  R+ L D V+EVRDARIP+SS  E +++   + +RII +NK DLA+
Sbjct: 28  WFPGHMAAATRAIRNRLKLSDLVIEVRDARIPLSSANEDLQSQMSAKRRIIALNKKDLAN 87

Query: 87  RSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLNFLQARVRELKKSGHSSHATTMMLVGI 146
            +    WT++FE        +NAH++ ++ + L+ ++ +++E+           +M+VG+
Sbjct: 88  PNVLNKWTRHFESSKQDCIAINAHSRSSVMKLLDLVELKLKEVIA---REPTLLVMVVGV 147

Query: 147 PNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGETKNISSLKIASHPNIYVLDTPGI 206
           PNVGKSAL NS+HQI       + +LK A V P PG T++I+  KIA  P+IYVLD+PG+
Sbjct: 148 PNVGKSALINSIHQIAAARFPVQERLKRATVGPLPGVTQDIAGFKIAHRPSIYVLDSPGV 207

Query: 207 LPPKILNIEVCSKLALTGAIRDILVGERVIVQYLLTILNSSDKYKKWASL-SATNCDSSL 266
           L P I +IE   KLAL+G+++D +VGE  I QY L ILN       W  L    N     
Sbjct: 208 LVPSIPDIETGLKLALSGSVKDSVVGEERIAQYFLAILNIRGTPLHWKYLVEGINEGPHA 267

Query: 267 EC-----STCSNSESQKRGYPSDHTQDFI---VNDVRRILFETISSFDGKTEDEKDMGNF 326
           +C         +   Q+   P      ++   +++V+R L+ T+S FDG TEDE D+   
Sbjct: 268 DCIDKPSYNLKDLRHQRTKQPDSSALHYVGDMISEVQRSLYITLSEFDGDTEDENDLECL 327

Query: 327 IEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYTLDSVP 366
           IE Q   L KAL +P  +S +A   V+ K L L+RTGRLG + LD VP
Sbjct: 328 IEQQFEVLQKALKIPHKAS-EARLMVSKKFLTLFRTGRLGPFILDDVP 371

[Close]

BLAST of MC05g0252 vs. TAIR 10
Match: AT4G02790.1 (GTP-binding family protein )

HSP 1 Score: 83.2 bits (204), Expect = 4.8e-16
Identity = 55/200 (27.50%), Postives = 95/200 (47.50%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPVSSEYEIMKNHSPSSKRIIVMNKVDLAD 86
           WY  H+    + + E++ L+D V+EVRDARIP+S+ +  M     + KRI+V+N+ D+  
Sbjct: 98  WYPGHIMKTEKELREQLKLMDVVIEVRDARIPLSTTHPKMDAWLGNRKRILVLNREDMIS 157

Query: 87  RSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLNFLQARVREL----KKSGHSSHATTMM 146
                 W +YF          N        +     ++   ++    ++ G    +    
Sbjct: 158 NDDRNDWARYFAKQGIKVIFTNGKLGMGAMKLGRLAKSLAGDVNGKRREKGLLPRSVRAG 217

Query: 147 LVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPEPGETKNISSLKIASHPNIYVLD 206
           ++G PNVGKS+L N L            K K    +P PG T+ +  +K+    ++ +LD
Sbjct: 218 IIGYPNVGKSSLINRLL-----------KRKICAAAPRPGVTREMKWVKLGK--DLDLLD 277

Query: 207 TPGILPPKILNIEVCSKLAL 223
           +PG+LP +I +     KLA+
Sbjct: 278 SPGMLPMRIDDQAAAIKLAI 284

[Close]

BLAST of MC05g0252 vs. TAIR 10
Match: AT3G07050.1 (GTP-binding family protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.7e-08
Identity = 54/190 (28.42%), Postives = 88/190 (46.32%), Query Frame = 0


Query: 43  IPLVDFVLEVRDARIPVSSEYEIMK----NHSPSSKRIIVMNKVDLADRSQTEVWTKYF- 102
           I L D +LEV DAR P+ +    M+       P+   ++++NK+DL  R   E W  Y  
Sbjct: 134 IELSDVILEVLDARDPLGTRCTDMERMVMQAGPNKHLVLLLNKIDLVPREAAEKWLMYLR 193

Query: 103 EDHNCVSY--------------GVNAHNKDNIREFLNFLQA-RVRELKKSGHSSH----A 162
           E+   V++                 A    N+ +  + L A  + +L K+   SH    +
Sbjct: 194 EEFPAVAFKCSTQEQRSNLGWKSSKASKPSNMLQTSDCLGADTLIKLLKNYSRSHELKKS 253

Query: 163 TTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAV-VSPEPGETKNISSLKIASHPN 208
            T+ ++G+PNVGKS+L NSL            K  H V V   PG T+++  + +    N
Sbjct: 254 ITVGIIGLPNVGKSSLINSL------------KRAHVVNVGATPGLTRSLQEVHL--DKN 309

The following BLAST results are available for this feature:

BLAST of MC05g0252 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82497	8.6e-119	62.05	DAR GTPase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DGP2 PE=3 SV=1	[more]
Q8L607	6.7e-63	40.23	Short integuments 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SIN2 PE=1 ...	[more]
Q9BT17	1.7e-26	33.33	Mitochondrial ribosome-associated GTPase 1 OS=Homo sapiens OX=9606 GN=MTG1 PE=1 ...	[more]
A9JTX2	5.0e-26	33.06	Mitochondrial ribosome-associated GTPase 1 OS=Xenopus tropicalis OX=8364 GN=mtg1...	[more]
E3TDS3	6.6e-26	33.05	Mitochondrial ribosome-associated GTPase 1 OS=Ictalurus punctatus OX=7998 GN=mtg...	[more]

BLAST of MC05g0252 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146907.1	4.15e-262	100.00	DAR GTPase 2, mitochondrial isoform X1 [Momordica charantia]	[more]
XP_022146908.1	1.32e-229	90.49	DAR GTPase 2, mitochondrial isoform X2 [Momordica charantia]	[more]
XP_023546448.1	4.37e-220	83.97	DAR GTPase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_038884377.1	7.23e-219	86.58	DAR GTPase 2, mitochondrial [Benincasa hispida]	[more]
XP_022962198.1	2.94e-218	83.15	DAR GTPase 2, mitochondrial isoform X1 [Cucurbita moschata]	[more]

BLAST of MC05g0252 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D0W3	2.01e-262	100.00	DAR GTPase 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CZT5	6.38e-230	90.49	DAR GTPase 2, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1HE44	1.42e-218	83.15	DAR GTPase 2, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1KB62	6.01e-218	83.15	DAR GTPase 2, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114922...	[more]
A0A1S3BA35	3.55e-212	83.61	DAR GTPase 2, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487836 P...	[more]

BLAST of MC05g0252 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10650.1	6.1e-120	62.05	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G41670.1	4.8e-64	40.23	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT4G02790.1	4.8e-16	27.50	GTP-binding family protein	[more]
AT3G07050.1	1.7e-08	28.42	GTP-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006073	GTP binding domain	PFAM	PF01926	MMR_HSR1	coord: 141..215 e-value: 2.4E-11 score: 43.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 26..207 e-value: 2.6E-34 score: 120.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 31..220
None	No IPR available	PIRSR	PIRSR006230-1	PIRSR006230-1	coord: 27..283 e-value: 4.4E-49 score: 164.3
None	No IPR available	PANTHER	PTHR45782:SF1	DAR GTPASE 2, MITOCHONDRIAL	coord: 3..367
None	No IPR available	PANTHER	PTHR45782	MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1	coord: 3..367
None	No IPR available	CDD	cd01856	YlqF	coord: 27..207 e-value: 1.17234E-59 score: 188.506
IPR030378	Circularly permuted (CP)-type guanine nucleotide-binding (G) domain	PROSITE	PS51721	G_CP	coord: 32..209 score: 34.3106

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0252.1	MC05g0252.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005743	mitochondrial inner membrane
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding