MC01g1267 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g1267
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDNA oxidative demethylase ALKBH2
LocationMC01: 17624028 .. 17624214 (-)
RNA-Seq ExpressionMC01g1267
SyntenyMC01g1267
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAGAATCGGAGACGACGGCCCCTCACCCTTCTGATTTAGGAAGGCGACGCAGATTGGATCTGGGAACCTGAAATTGAAGCGAAATCAAGCAGGTTTGTGCTGTACGGCGTCTCCGATCAGGCTTGGACGTGGTTCGATTGTCTCAATCGACGCATTCCATGGACCGGGCCAACCATTCGCGTCTTC

mRNA sequence

TCAGAATCGGAGACGACGGCCCCTCACCCTTCTGATTTAGGAAGGCGACGCAGATTGGATCTGGGAACCGAAATTGAAGCGAAATCAAGCAGGTTTGTGCTGTACGGCGTCTCCGATCAGGCTTGGACGTGGTTCGATTGTCTCAATCGACGCATTCCATGGACCGGGCCAACCATTCGCGTCTTC

Coding sequence (CDS)

TCAGAATCGGAGACGACGGCCCCTCACCCTTCTGATTTAGGAAGGCGACGCAGATTGGATCTGGGAACCGAAATTGAAGCGAAATCAAGCAGGTTTGTGCTGTACGGCGTCTCCGATCAGGCTTGGACGTGGTTCGATTGTCTCAATCGACGCATTCCATGGACCGGGCCAACCATTCGCGTCTTC

Protein sequence

SESETTAPHPSDLGRRRRLDLGTEIEAKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTIRVF
Homology
[Close]
BLAST of MC01g1267 vs. ExPASy Swiss-Prot
Match: Q9SIE0 (DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 1.6e-04
Identity = 24/57 (42.11%), Postives = 35/57 (61.40%), Query Frame = 0 

Query: 7   APHPSDLGRRRRLDLGTEIE-AKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTIRVF 63
           +P  S+   R+ +DLG   +     RF+ +    Q+WT+FD L++ IPWT PTIRVF
Sbjct: 87  SPSSSNAPSRKTIDLGHGSDLIYIQRFLPF---QQSWTFFDYLDKHIPWTRPTIRVF 140

[Close]
BLAST of MC01g1267 vs. NCBI nr
Match: XP_023517872.1 (DNA oxidative demethylase ALKBH2-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 75.5 bits (184), Expect = 4.27e-16
Identity = 37/61 (60.66%), Postives = 42/61 (68.85%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIEAKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTIR 60
          SESETT P P D+GRR+ LDLG   E       ++  SDQAWTWFD LN+ IPWT PTIR
Sbjct: 4  SESETTIPRPIDIGRRQTLDLGNGSEIVY--ITMFVPSDQAWTWFDFLNQHIPWTRPTIR 62

[Close]
BLAST of MC01g1267 vs. NCBI nr
Match: XP_023517871.1 (DNA oxidative demethylase ALKBH2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 75.5 bits (184), Expect = 4.39e-16
Identity = 37/61 (60.66%), Postives = 42/61 (68.85%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIEAKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTIR 60
          SESETT P P D+GRR+ LDLG   E       ++  SDQAWTWFD LN+ IPWT PTIR
Sbjct: 4  SESETTIPRPIDIGRRQTLDLGNGSEIVY--ITMFVPSDQAWTWFDFLNQHIPWTRPTIR 62

[Close]
BLAST of MC01g1267 vs. NCBI nr
Match: XP_023517870.1 (DNA oxidative demethylase ALKBH2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 75.5 bits (184), Expect = 5.14e-16
Identity = 37/61 (60.66%), Postives = 42/61 (68.85%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIEAKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTIR 60
          SESETT P P D+GRR+ LDLG   E       ++  SDQAWTWFD LN+ IPWT PTIR
Sbjct: 4  SESETTIPRPIDIGRRQTLDLGNGSEIVY--ITMFVPSDQAWTWFDFLNQHIPWTRPTIR 62

[Close]
BLAST of MC01g1267 vs. NCBI nr
Match: KAG6603836.1 (DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 77.0 bits (188), Expect = 3.65e-15
Identity = 41/63 (65.08%), Postives = 44/63 (69.84%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIE-AKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTI 60
          SESET  P P DLGRR+ LDLG   E    S+FV    SDQAWTWFD LN+ IPWT PTI
Sbjct: 11 SESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVS---SDQAWTWFDFLNQHIPWTRPTI 70

Query: 61 RVF 62
          RVF
Sbjct: 71 RVF 70

[Close]
BLAST of MC01g1267 vs. NCBI nr
Match: XP_022950477.1 (DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 DNA oxidative demethylase ALKBH2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 77.0 bits (188), Expect = 6.45e-15
Identity = 41/63 (65.08%), Postives = 44/63 (69.84%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIE-AKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTI 60
          SESET  P P DLGRR+ LDLG   E    S+FV    SDQAWTWFD LN+ IPWT PTI
Sbjct: 25 SESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVS---SDQAWTWFDFLNQHIPWTRPTI 84

Query: 61 RVF 62
          RVF
Sbjct: 85 RVF 84

[Close]
BLAST of MC01g1267 vs. ExPASy TrEMBL
Match: A0A6J1GEX9 (DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.12e-15
Identity = 41/63 (65.08%), Postives = 44/63 (69.84%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIE-AKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTI 60
          SESET  P P DLGRR+ LDLG   E    S+FV    SDQAWTWFD LN+ IPWT PTI
Sbjct: 25 SESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVS---SDQAWTWFDFLNQHIPWTRPTI 84

Query: 61 RVF 62
          RVF
Sbjct: 85 RVF 84

[Close]
BLAST of MC01g1267 vs. ExPASy TrEMBL
Match: A0A5A7SMW2 (DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G00710 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.57e-15
Identity = 40/63 (63.49%), Postives = 45/63 (71.43%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIEAKS-SRFVLYGVSDQAWTWFDCLNRRIPWTGPTI 60
          SE+ET  P P DLG+R+ LDLG   E    S+FV    SDQAWTWFD LN+RIPWT PTI
Sbjct: 11 SETETKIPRPFDLGKRQTLDLGNGSEILYISKFVS---SDQAWTWFDYLNQRIPWTRPTI 70

Query: 61 RVF 62
          RVF
Sbjct: 71 RVF 70

[Close]
BLAST of MC01g1267 vs. ExPASy TrEMBL
Match: A0A6J1GEX8 (DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.72e-15
Identity = 41/63 (65.08%), Postives = 44/63 (69.84%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIE-AKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTI 60
          SESET  P P DLGRR+ LDLG   E    S+FV    SDQAWTWFD LN+ IPWT PTI
Sbjct: 25 SESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVS---SDQAWTWFDFLNQHIPWTRPTI 84

Query: 61 RVF 62
          RVF
Sbjct: 85 RVF 84

[Close]
BLAST of MC01g1267 vs. ExPASy TrEMBL
Match: A0A6J1BTV9 (DNA oxidative demethylase ALKBH2 OS=Momordica charantia OX=3673 GN=LOC111005717 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.00e-15
Identity = 40/63 (63.49%), Postives = 44/63 (69.84%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIE-AKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTI 60
          SESET  P P DLGRR  LDLG   E    ++FV    SD+AWTWFD LN+RIPWT PTI
Sbjct: 19 SESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFV---PSDKAWTWFDFLNQRIPWTRPTI 78

Query: 61 RVF 62
          RVF
Sbjct: 79 RVF 78

[Close]
BLAST of MC01g1267 vs. ExPASy TrEMBL
Match: A0A6J1IQU8 (DNA oxidative demethylase ALKBH2 OS=Cucurbita maxima OX=3661 GN=LOC111477932 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.18e-15
Identity = 41/63 (65.08%), Postives = 44/63 (69.84%), Query Frame = 0 

Query: 1  SESETTAPHPSDLGRRRRLDLGTEIE-AKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTI 60
          SESET  P P DLGRR+ LDLG   E    S+FV    SDQAWTWFD LN+ IPWT PTI
Sbjct: 25 SESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVS---SDQAWTWFDFLNQHIPWTRPTI 84

Query: 61 RVF 62
          RVF
Sbjct: 85 RVF 84

[Close]
BLAST of MC01g1267 vs. TAIR 10
Match: AT2G22260.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 46.2 bits (108), Expect = 1.1e-05
Identity = 24/57 (42.11%), Postives = 35/57 (61.40%), Query Frame = 0 

Query: 7   APHPSDLGRRRRLDLGTEIE-AKSSRFVLYGVSDQAWTWFDCLNRRIPWTGPTIRVF 63
           +P  S+   R+ +DLG   +     RF+ +    Q+WT+FD L++ IPWT PTIRVF
Sbjct: 87  SPSSSNAPSRKTIDLGHGSDLIYIQRFLPF---QQSWTFFDYLDKHIPWTRPTIRVF 140

The following BLAST results are available for this feature:
BLAST of MC01g1267 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SIE01.6e-0442.11DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 ... [more]
BLAST of MC01g1267 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023517872.14.27e-1660.66DNA oxidative demethylase ALKBH2-like isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_023517871.14.39e-1660.66DNA oxidative demethylase ALKBH2-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023517870.15.14e-1660.66DNA oxidative demethylase ALKBH2-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6603836.13.65e-1565.08DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022950477.16.45e-1565.08DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 D... [more]
BLAST of MC01g1267 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GEX93.12e-1565.08DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A5A7SMW23.57e-1563.49DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1GEX83.72e-1565.08DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1BTV94.00e-1563.49DNA oxidative demethylase ALKBH2 OS=Momordica charantia OX=3673 GN=LOC111005717 ... [more]
A0A6J1IQU84.18e-1565.08DNA oxidative demethylase ALKBH2 OS=Cucurbita maxima OX=3661 GN=LOC111477932 PE=... [more]
BLAST of MC01g1267 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G22260.11.1e-0542.11oxidoreductase, 2OG-Fe(II) oxygenase family protein [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g1267.1MC01g1267.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006307 DNA dealkylation involved in DNA repair
biological_process GO:0035511 oxidative DNA demethylation
molecular_function GO:0051747 cytosine C-5 DNA demethylase activity
molecular_function GO:0043734 DNA-N1-methyladenine dioxygenase activity
molecular_function GO:0008198 ferrous iron binding