Tan0021985.1 (mRNA) Snake gourd v1

Overview
NameTan0021985.1
TypemRNA
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionExonuclease domain-containing protein
LocationLG09: 5227373 .. 5230108 (+)
Sequence length1494
RNA-Seq ExpressionTan0021985.1
SyntenyTan0021985.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAAAATCCAAGAACCAGTTTCTTCGGATTTCCATGTTTCCGAAAAGCACTTCTCCATCTTCTTCGCCACGATTTACACCCTCTCTCTCTCTCATTTAACTTACTTTCTTCTGTCGGGTGTTCAAAAAATGCAGCAAAATCCTCCCTCAGATTTCCTTTCAGGCAAGTTTCTTTCTCTCTCTCTTATTTTTTCTGCTAGTTTCCTGTTTCTGCATTGAAAAAATGGTTCAAAATGTGAAAAAAAGGGGAAGGAATTCTGTTCTTCTGAAATACGAATGTAGATGGTTCGAAGTTGTTCAACAATTTAATGGGTCTATTGCAGCTTGCACTGTATACCCTTTTTTTTCAATCTGAGGCAGCAGGTCTTAATTTGGAAGTTTTGAGTGTGAGCAATCAATTGGTTTGTGTTGCTTGATTTCGTGTTTATTTGATTTTTACTAATGAATGATTGGATTTCTCTTTGTGGGAATTCGGGGAAGTATTGGACATGGCGGGAGTTGGAAATGTTCTTGGCTAAAGGGATTTGATTACAAAGTGTTTGTGAAAATGCCTGAATGAGCTCATGGAAGATTAATTCTATTTTTTTTGCTTTGACATAGTTGTGTTTGGGAAGGAATTCTGTTCTTCTGAAATACGAATGTAGATGGTTCGAAGTTGTTCAACAATTTAATGGGTCTATTGCAGCTTGCACTGTATACCCTTTTTTTTCAATCTGAGGCAGCAGGTCTTAATTTGGAAGTTTTGAGTGTGAGCAATCAATTGGTTTGTGTTGCTTGATTTCGTGTTTATTTGATTTTTACTAATGAATGATTGGATTTCTCTTTGTGGGAATTCAGGGAAGTATTGGACATGGCGGGAGTTGGAAATGTTCTTGGCTAAAGGGATTTGATTACAAAGTGTTTGTGAAAATGCCTGAATGAGCTCATGGAAGACTAATTCTATTTTTTTTGCTTTGACATAGTTGTGTTTGTTCTTTTGAGCATTTGGGCTTTTAACTTTGTTGTTTTGTTGGTTCTCTGGTGATTCTGTTTGATAATTAATGTGAAGAAATTGAAGATGATCTTTTTTTTGTTTCTTGGTCTGATAATGGTCTTTTTTACATTGTTTGTCACCCCCCATTATCTTCAGGTTGAAGAAAATTTCTTCCGATGCTGGATTGTTTTGGGTTTTCTGTCTAGTTTTCCTCTTGTTGTGTTGTGATTTCAAATGATAACAGTTCCGATGTGCTTTTCAATCTTGCGAGTTCCGAAATTTGGAGTCCACACTTTAGCTAATTTATGGAGGGTAAACTTCCATCCCTTGAAGAGGAATTATGGTCATAGTTGTTGGTATGATCAGTTTGGTTCTAGAATCTATAGCCTTGAAGGAGGACCGAATAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCAAAAACCAACCCTAGCAGTAAACCAAGCAATATCAGGAATGAAATCTTAGAGGAACCGATCTCAACAAGCTGTGCAGTTAGCATTAATAAGTCTGAAAAGAGTGAAACTCAGAAGCTTCAATATTGTGATATTCAGCAAACGATTATTAAGAACAAGGAATTGGCTCGACCGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAGACCTTGATAGAATAGTGGAAATTGCACTTCAAGATCTTTCAGGCGGAGAAAATAGCACCTTCCAAACATTAATAAATCCAGAGTGCTATATTCCAAATTCAGTTGTACATGGCATTACAAGCCGAATGGTCAGTAGACCCGATGTTCCAAGGTATCGAAACGCTTGTTGTAGTCTCATTCCTCTAATATTGGCTCCAATTTGAATCTTGGATTAGGTTTATGCTAGGTTCCCTCATTTCAAATTTTCAAAGTCATTATATTTTATTTACTGTGATATTTCCAGAGTTGATCAATCATATCTAAACGTTTCTTTTCTATGTTCATATAGGATGAAGGAACTCATACCAATTTTATTACAATTTATTAGAAGCCGTCAGAAACCTGATGGTTATGTATTGTTAGTAGCTCATAATGCTCGTACATTCGACGTGCCTTTTTTGCTCAAGGAATTCAAACGTTGCTCGGTTGACATTCCTCCAAATTGGCTATTTTTCGATAACATTCTTTTAGCCCGTCAATTAATGAAATCCTCAGGTTAGTTTTCCCTTTGCACATATTATCTTGCTCTGGTTTCTTTGATGATAAAAAGATTGATGTGATGTAATGTAATATCGATGGAAATGTCGTGATCAGATTCGAAGATTACGAAAATATCATTACAAGCTCTTCGTGAGTATTACAATATTCCACTGGTGGGTTCAGCTCACAGAGCAATGTCCGACGTGTGGTCGTTGTCGATGATTCTTCAGAGGCTTACTTTTGAGCTCAAGCTAGGCATCCCGGATCTCGTTGAAAGATCTTTCACAGCATCAGATCTGATTAATAAGAAGAAGAATTAGTGTGTTAAGTTATATGCACAAATCAATCTTAACTTGGTGATTAAGCTGTGCTTTTGTTGTAATGTTATTTATTAATTGTAACTTCAGTTCATTCTTATCAGCAAAATCCTGGGCCATTTTATTTATGGTCACTATATTTGTCTTGATTTCCATATTCTCCAAAAACTAATGCCTGAATTGGAATATCACGACAGACAAAGTATAGTTTTGTTTTCTTGCTTTGAATGGATACCGAATGAAACAAGAATTGATAGA

mRNA sequence

CTAAAATCCAAGAACCAGTTTCTTCGGATTTCCATGTTTCCGAAAAGCACTTCTCCATCTTCTTCGCCACGATTTACACCCTCTCTCTCTCTCATTTAACTTACTTTCTTCTGTCGGGTGTTCAAAAAATGCAGCAAAATCCTCCCTCAGATTTCCTTTCAGGTTGAAGAAAATTTCTTCCGATGCTGGATTGTTTTGGGTTTTCTGTCTAGTTTTCCTCTTGTTGTGTTGTGATTTCAAATGATAACAGTTCCGATGTGCTTTTCAATCTTGCGAGTTCCGAAATTTGGAGTCCACACTTTAGCTAATTTATGGAGGGTAAACTTCCATCCCTTGAAGAGGAATTATGGTCATAGTTGTTGGTATGATCAGTTTGGTTCTAGAATCTATAGCCTTGAAGGAGGACCGAATAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCAAAAACCAACCCTAGCAGTAAACCAAGCAATATCAGGAATGAAATCTTAGAGGAACCGATCTCAACAAGCTGTGCAGTTAGCATTAATAAGTCTGAAAAGAGTGAAACTCAGAAGCTTCAATATTGTGATATTCAGCAAACGATTATTAAGAACAAGGAATTGGCTCGACCGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAGACCTTGATAGAATAGTGGAAATTGCACTTCAAGATCTTTCAGGCGGAGAAAATAGCACCTTCCAAACATTAATAAATCCAGAGTGCTATATTCCAAATTCAGTTGTACATGGCATTACAAGCCGAATGGTCAGTAGACCCGATGTTCCAAGGATGAAGGAACTCATACCAATTTTATTACAATTTATTAGAAGCCGTCAGAAACCTGATGGTTATGTATTGTTAGTAGCTCATAATGCTCGTACATTCGACGTGCCTTTTTTGCTCAAGGAATTCAAACGTTGCTCGGTTGACATTCCTCCAAATTGGCTATTTTTCGATAACATTCTTTTAGCCCGTCAATTAATGAAATCCTCAGATTCGAAGATTACGAAAATATCATTACAAGCTCTTCGTGAGTATTACAATATTCCACTGGTGGGTTCAGCTCACAGAGCAATGTCCGACGTGTGGTCGTTGTCGATGATTCTTCAGAGGCTTACTTTTGAGCTCAAGCTAGGCATCCCGGATCTCGTTGAAAGATCTTTCACAGCATCAGATCTGATTAATAAGAAGAAGAATTAGTGTGTTAAGTTATATGCACAAATCAATCTTAACTTGGTGATTAAGCTGTGCTTTTGTTGTAATGTTATTTATTAATTGTAACTTCAGTTCATTCTTATCAGCAAAATCCTGGGCCATTTTATTTATGGTCACTATATTTGTCTTGATTTCCATATTCTCCAAAAACTAATGCCTGAATTGGAATATCACGACAGACAAAGTATAGTTTTGTTTTCTTGCTTTGAATGGATACCGAATGAAACAAGAATTGATAGA

Coding sequence (CDS)

ATGATAACAGTTCCGATGTGCTTTTCAATCTTGCGAGTTCCGAAATTTGGAGTCCACACTTTAGCTAATTTATGGAGGGTAAACTTCCATCCCTTGAAGAGGAATTATGGTCATAGTTGTTGGTATGATCAGTTTGGTTCTAGAATCTATAGCCTTGAAGGAGGACCGAATAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCAAAAACCAACCCTAGCAGTAAACCAAGCAATATCAGGAATGAAATCTTAGAGGAACCGATCTCAACAAGCTGTGCAGTTAGCATTAATAAGTCTGAAAAGAGTGAAACTCAGAAGCTTCAATATTGTGATATTCAGCAAACGATTATTAAGAACAAGGAATTGGCTCGACCGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAGACCTTGATAGAATAGTGGAAATTGCACTTCAAGATCTTTCAGGCGGAGAAAATAGCACCTTCCAAACATTAATAAATCCAGAGTGCTATATTCCAAATTCAGTTGTACATGGCATTACAAGCCGAATGGTCAGTAGACCCGATGTTCCAAGGATGAAGGAACTCATACCAATTTTATTACAATTTATTAGAAGCCGTCAGAAACCTGATGGTTATGTATTGTTAGTAGCTCATAATGCTCGTACATTCGACGTGCCTTTTTTGCTCAAGGAATTCAAACGTTGCTCGGTTGACATTCCTCCAAATTGGCTATTTTTCGATAACATTCTTTTAGCCCGTCAATTAATGAAATCCTCAGATTCGAAGATTACGAAAATATCATTACAAGCTCTTCGTGAGTATTACAATATTCCACTGGTGGGTTCAGCTCACAGAGCAATGTCCGACGTGTGGTCGTTGTCGATGATTCTTCAGAGGCTTACTTTTGAGCTCAAGCTAGGCATCCCGGATCTCGTTGAAAGATCTTTCACAGCATCAGATCTGATTAATAAGAAGAAGAATTAG

Protein sequence

MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRWTRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQTIIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSVVHGITSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQRLTFELKLGIPDLVERSFTASDLINKKKN
Homology
BLAST of Tan0021985.1 vs. ExPASy Swiss-Prot
Match: Q682U6 (Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DPD1 PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.7e-62
Identity = 138/323 (42.72%), Postives = 202/323 (62.54%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRWTRKSV 65
           MC SI +V +  +H+  +      H   +N   S        R  S++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 66  TTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQTIIKNK 125
           +T       T   S+ SN ++ +L   +  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST-------TTQGSR-SNTKSSVLGGTVPVTRII----DEESRTKVQPFGNLQQRLAQDK 120

Query: 126 ELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECY-IPNSVVHGI 185
           +L++ +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDI 245
            + MV RP+VPRM+ELIPI L+++ SRQKP GYV+LVAHN ++FD  FL+ EF RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PPNWLFFDNILLARQLMKSSDSKI-TKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY++   G AHRA+SDV  LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFELKLGIPDLVERSFTASDL 327
           +LT +LKL + DLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Tan0021985.1 vs. ExPASy Swiss-Prot
Match: Q895K2 (DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=polC PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.4e-13
Identity = 60/180 (33.33%), Postives = 91/180 (50.56%), Query Frame = 0

Query: 134 ITFDIETTGFSRDLDRIVEI-ALQDLSGGENSTFQTLINPECYIPNSVVH--GITSRMVS 193
           + FDIETTGFS   D+I+EI A++   G     + T +NPE  IP  ++   GI   MV 
Sbjct: 414 VVFDIETTGFSNKNDKIIEIGAVKIKEGKVVDKYSTFVNPERIIPEKIIELTGIHDYMVK 473

Query: 194 RPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDIPPNWLF 253
             D P+++E++P  + FI          +LVAHNA  FDV F+ K  K   +       F
Sbjct: 474 --DAPKIEEVLPKFIDFIEDS-------ILVAHNA-NFDVSFIKKNCKDFGIH------F 533

Query: 254 FDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQRLTFELK 311
            + IL    L K    ++ +  L  + ++  IPL+ + HRA+ D  +   IL +   +LK
Sbjct: 534 NNPILDTIPLCKFLYPELKRYKLNVVAKHLGIPLL-NHHRAVEDAKTTGDILLKAFEDLK 576

BLAST of Tan0021985.1 vs. ExPASy Swiss-Prot
Match: Q8RA32 (DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=polC PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 5.4e-11
Identity = 57/189 (30.16%), Postives = 96/189 (50.79%), Query Frame = 0

Query: 134 ITFDIETTGFSRDLDRIVEIALQDLSGGE-NSTFQTLINPECYIPNSV--VHGITSRMVS 193
           + FDIETTG S   D I+EI    +   +   TF+T +NP+  I + +  + GI   MV 
Sbjct: 389 VVFDIETTGLSNINDEIIEIGAVKIKNKKIVDTFETFVNPQIPISSFITKLTGIDESMVK 448

Query: 194 RPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDIPPNWLF 253
             D P ++E++P  L+F +         +LVAHNA  FDV F+  + K+  + +    L 
Sbjct: 449 --DAPLIEEVLPKFLEFAKG-------AVLVAHNA-NFDVSFIKSKAKKLGLTVENTVL- 508

Query: 254 FDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQRLTFEL- 313
            D + L+R L +     +    L  L E++ + L+   HRA+ D  + + I  ++  +L 
Sbjct: 509 -DTLELSRHLYQD----LKNYKLDTLAEFFEVKLL-HHHRAVEDAKATAEIFIKMLEKLQ 560

Query: 314 KLGIPDLVE 319
           ++GI  + E
Sbjct: 569 EIGIKSVSE 560

BLAST of Tan0021985.1 vs. ExPASy Swiss-Prot
Match: Q8XJR3 (DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=polC PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.2e-10
Identity = 56/173 (32.37%), Postives = 85/173 (49.13%), Query Frame = 0

Query: 134 ITFDIETTGFSRDLDRIVEIALQDLSGGE-NSTFQTLINPECYIPNSVVH--GITSRMVS 193
           + FDIETTGFS   D+I+EI    L  GE   +F T ++P+  IP  +     IT  MV+
Sbjct: 436 VVFDIETTGFSSKNDKIIEIGAVKLKDGEIVDSFSTFVDPKVNIPYKITELTSITQNMVN 495

Query: 194 RPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDIPPNWLF 253
               P + E++P  ++F+       G  +LVAHNA  FDV F+ K        +      
Sbjct: 496 --GQPTIDEVLPKFMEFV-------GNSVLVAHNA-AFDVGFIKKNLMDMGKTLKNP--V 555

Query: 254 FDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQ 304
            D + LAR L       + K+ L  + ++  I L  + HRA+ D  + + IL+
Sbjct: 556 MDTVPLARYLYPD----LKKVKLNLVAKHLGISL-ENHHRAVDDAKATAEILK 591

BLAST of Tan0021985.1 vs. ExPASy Swiss-Prot
Match: A7X1P4 (DNA polymerase III PolC-type OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) OX=418127 GN=polC PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-07
Identity = 48/189 (25.40%), Postives = 93/189 (49.21%), Query Frame = 0

Query: 129 RPVTVITFDIETTGFSRDLDRIVEIALQDLSGGE-NSTFQTLINPECYIPNSVVH--GIT 188
           +  T + FD+ETTG S   D+I+E+A   +  GE    F+   NP   +  ++++   IT
Sbjct: 418 KDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEIIDKFERFSNPHERLSETIINLTHIT 477

Query: 189 SRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDIP 248
             M+   D P ++E++    +++       G  + VAHNA +FD+ F+   ++R      
Sbjct: 478 DDML--VDAPEIEEVLTEFKEWV-------GDAIFVAHNA-SFDMGFIDTGYERLGFGPS 537

Query: 249 PNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQRL 308
            N      ++   +L ++ +++  K  L  L + Y + L    HRA+ D  + + I  ++
Sbjct: 538 TN-----GVIDTLELSRTINTEYGKHGLNFLAKKYGVELT-QHHRAIYDTEATAYIFIKM 590

Query: 309 TFELK-LGI 314
             ++K LG+
Sbjct: 598 VQQMKELGV 590

BLAST of Tan0021985.1 vs. NCBI nr
Match: KAG6590475.1 (Exonuclease DPD1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024006.1 Exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 297/332 (89.46%), Postives = 316/332 (95.18%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRW 60
           MIT PMCFSILRVPKFGV TLANLWR NFHPL RNYGHS WYDQ G RIY+LEGG +KRW
Sbjct: 1   MITAPMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQT 120
           TRKS++TKAGRKAKT+PSSKPSNIRNEILEE +STSC VSINKSEK+ TQKLQ C+IQ+T
Sbjct: 61  TRKSMSTKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQET 120

Query: 121 IIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180
           I+K+KELARPVTVITFDIETTGFSR+LDRIVEIALQDLSGGENSTFQTLINPECYIPN+V
Sbjct: 121 IVKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 VHGITSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRC 240
           VHGITSRMVSRPDVPRMKELIPILLQFI+SRQKPDGYVLLVAHNARTFDVPFLLKEF RC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRC 240

Query: 241 SVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSM 300
           SVDIP NWLFFDNILLARQLMKS+DSKITKISLQALREYYNIPLVGSAHRAMSDV +LS+
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFELKLGIPDLVERSFTASDLINKKKN 333
           ILQRLTF+LKLGIPDL+ERSFT SDLINKKKN
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Tan0021985.1 vs. NCBI nr
Match: XP_022961373.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata] >XP_022961374.1 exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 594.3 bits (1531), Expect = 6.3e-166
Identity = 296/332 (89.16%), Postives = 315/332 (94.88%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRW 60
           MIT PMCFSILRVPKFGV TLANLWR NFHPL RNYGHS WYDQ G R Y+LEGG +KRW
Sbjct: 1   MITAPMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRTYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQT 120
           TRKS++TKAGRKAKT+PSSKPSNIRNEILEE +STSC VSINKSEK+ TQKLQ C+IQ+T
Sbjct: 61  TRKSMSTKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQET 120

Query: 121 IIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180
           I+K+KELARPVTVITFDIETTGFSR+LDRIVEIALQDLSGGENSTFQTLINPECYIPN+V
Sbjct: 121 IVKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 VHGITSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRC 240
           VHGITSRMVSRPDVPRMKELIPILLQFI+SRQKPDGYVLLVAHNARTFDVPFLLKEF RC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRC 240

Query: 241 SVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSM 300
           SVDIP NWLFFDNILLARQLMKS+DSKITKISLQALREYYNIPLVGSAHRAMSDV +LS+
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFELKLGIPDLVERSFTASDLINKKKN 333
           ILQRLTF+LKLGIPDL+ERSFT SDLINKKKN
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Tan0021985.1 vs. NCBI nr
Match: XP_023515555.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594.0 bits (1530), Expect = 8.2e-166
Identity = 296/332 (89.16%), Postives = 314/332 (94.58%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRW 60
           MIT PMCFSILRVPKFGV TLANLWR NFHPL RNYGHS WYDQ G RIY+LEGG NKRW
Sbjct: 1   MITAPMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQNKRW 60

Query: 61  TRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQT 120
           TRKS++TKAGRKAKT+PSSKPSNIRNEILEE +STSC +SINKSEK+ TQKLQ C+IQ+T
Sbjct: 61  TRKSMSTKAGRKAKTDPSSKPSNIRNEILEESVSTSCTISINKSEKNGTQKLQCCNIQET 120

Query: 121 IIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180
           I K+KELARPVTVITFDIETTGFSR+LDRIVEIALQDLSGGENSTFQTLINPECYIPN+V
Sbjct: 121 ITKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 VHGITSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRC 240
           VHGITSRMVSRPDVPRMKELIPILLQFI+SRQKPDGYVLLVAHNARTFDVPFLLKEF RC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRC 240

Query: 241 SVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSM 300
           SVDIP NWLFFDNILLARQLMKS+DSKI KISLQALREYYNIPLVGSAHRAMSDV +LS+
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKIKKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFELKLGIPDLVERSFTASDLINKKKN 333
           ILQRLTF+LKLGIPDL+ERSFT SDLINKKKN
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Tan0021985.1 vs. NCBI nr
Match: XP_022968752.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1 exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 590.9 bits (1522), Expect = 6.9e-165
Identity = 296/331 (89.43%), Postives = 312/331 (94.26%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRW 60
           MIT  MCFSILRVPKFGV TLANLWR NFHPL RNYGHS WYDQ G RIY+LEGG +KRW
Sbjct: 1   MITASMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQT 120
           TRKS+TTKAGRKAKTNPSSKPSNIRNEILEE +STSC VSINKSEK  TQKLQ C+IQ+T
Sbjct: 61  TRKSMTTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQET 120

Query: 121 IIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180
           IIK+KELARPVTVITFDIETTGFSR+LDRIVEIALQDLSGGENSTFQTLINPECYIPN+V
Sbjct: 121 IIKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 VHGITSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRC 240
           VHGITSRMVSRPDVPRMKELIPILLQF++SRQKPDGYVLLVAHNAR FDVPFLLKEF RC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRC 240

Query: 241 SVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSM 300
           SVDIP NWLFFDNILLARQLMKS+DSKITKISLQALREYYNIPLVGSAHRAMSDV +LS+
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFELKLGIPDLVERSFTASDLINKKK 332
           ILQRLTF+LKLGIPDL+ERSFT SDLINKKK
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of Tan0021985.1 vs. NCBI nr
Match: XP_038878496.1 (exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP_038878497.1 exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 577.4 bits (1487), Expect = 7.9e-161
Identity = 295/334 (88.32%), Postives = 306/334 (91.62%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRW 60
           MI  PMCFSILR PKFGV TLANLWR NFHPL RN  HS W+DQ G RIY+LEGG NKRW
Sbjct: 1   MILAPMCFSILRFPKFGVPTLANLWRENFHPLNRNCYHSSWHDQLGFRIYNLEGGQNKRW 60

Query: 61  TRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQT 120
            RKS+TTKAGRKAK NPSSKP+NIRNEILEE ISTSC VSINKSEKSETQKLQYCDIQQ 
Sbjct: 61  IRKSMTTKAGRKAKANPSSKPTNIRNEILEESISTSCTVSINKSEKSETQKLQYCDIQQL 120

Query: 121 IIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180
           IIKNKELARPVTVITFDIETTGFSR+LDRIVEIALQDLSGGENSTFQTLINPECYIPNSV
Sbjct: 121 IIKNKELARPVTVITFDIETTGFSRNLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180

Query: 181 VHGITSRMVSRPDVPRMKELIPILLQFIRSR--QKPDGYVLLVAHNARTFDVPFLLKEFK 240
           VHGIT+ MVSRPDVPRMKELIPILLQF+ SR  QKPDGYVLLVAHNARTFDVPFLLKEF 
Sbjct: 181 VHGITNHMVSRPDVPRMKELIPILLQFVESRQKQKPDGYVLLVAHNARTFDVPFLLKEFS 240

Query: 241 RCSVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSL 300
           RCS DIPPNW FFD+I LARQLMKSSDSKITKISLQALREYY+I LVGSAHRAMSDV SL
Sbjct: 241 RCSFDIPPNWRFFDSIPLARQLMKSSDSKITKISLQALREYYDIQLVGSAHRAMSDVRSL 300

Query: 301 SMILQRLTFELKLGIPDLVERSFTASDLINKKKN 333
           S+ILQRLTFELKLGI DL+ERSFTASDLINKKKN
Sbjct: 301 SLILQRLTFELKLGISDLIERSFTASDLINKKKN 334

BLAST of Tan0021985.1 vs. ExPASy TrEMBL
Match: A0A6J1HDU6 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461962 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.0e-166
Identity = 296/332 (89.16%), Postives = 315/332 (94.88%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRW 60
           MIT PMCFSILRVPKFGV TLANLWR NFHPL RNYGHS WYDQ G R Y+LEGG +KRW
Sbjct: 1   MITAPMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRTYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQT 120
           TRKS++TKAGRKAKT+PSSKPSNIRNEILEE +STSC VSINKSEK+ TQKLQ C+IQ+T
Sbjct: 61  TRKSMSTKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQET 120

Query: 121 IIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180
           I+K+KELARPVTVITFDIETTGFSR+LDRIVEIALQDLSGGENSTFQTLINPECYIPN+V
Sbjct: 121 IVKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 VHGITSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRC 240
           VHGITSRMVSRPDVPRMKELIPILLQFI+SRQKPDGYVLLVAHNARTFDVPFLLKEF RC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRC 240

Query: 241 SVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSM 300
           SVDIP NWLFFDNILLARQLMKS+DSKITKISLQALREYYNIPLVGSAHRAMSDV +LS+
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFELKLGIPDLVERSFTASDLINKKKN 333
           ILQRLTF+LKLGIPDL+ERSFT SDLINKKKN
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Tan0021985.1 vs. ExPASy TrEMBL
Match: A0A6J1HUD7 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467897 PE=4 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.3e-165
Identity = 296/331 (89.43%), Postives = 312/331 (94.26%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRW 60
           MIT  MCFSILRVPKFGV TLANLWR NFHPL RNYGHS WYDQ G RIY+LEGG +KRW
Sbjct: 1   MITASMCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRW 60

Query: 61  TRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQT 120
           TRKS+TTKAGRKAKTNPSSKPSNIRNEILEE +STSC VSINKSEK  TQKLQ C+IQ+T
Sbjct: 61  TRKSMTTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQET 120

Query: 121 IIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180
           IIK+KELARPVTVITFDIETTGFSR+LDRIVEIALQDLSGGENSTFQTLINPECYIPN+V
Sbjct: 121 IIKDKELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAV 180

Query: 181 VHGITSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRC 240
           VHGITSRMVSRPDVPRMKELIPILLQF++SRQKPDGYVLLVAHNAR FDVPFLLKEF RC
Sbjct: 181 VHGITSRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRC 240

Query: 241 SVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSM 300
           SVDIP NWLFFDNILLARQLMKS+DSKITKISLQALREYYNIPLVGSAHRAMSDV +LS+
Sbjct: 241 SVDIPLNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSL 300

Query: 301 ILQRLTFELKLGIPDLVERSFTASDLINKKK 332
           ILQRLTF+LKLGIPDL+ERSFT SDLINKKK
Sbjct: 301 ILQRLTFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of Tan0021985.1 vs. ExPASy TrEMBL
Match: A0A6J1C1V8 (exonuclease DPD1, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111007515 PE=4 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.3e-158
Identity = 281/332 (84.64%), Postives = 300/332 (90.36%), Query Frame = 0

Query: 1   MITVPMCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRW 60
           MITVPMCFSILRVPKFGVHTLAN WR NFHPL RNYGHS WYDQ   RIY+LEGG NKRW
Sbjct: 1   MITVPMCFSILRVPKFGVHTLANFWRTNFHPLNRNYGHSSWYDQLCFRIYNLEGGQNKRW 60

Query: 61  TRKSVTTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQT 120
           TRKS+TTKAGRK K NPSSKPS+IRNEILE  +STSC VSINK+EKSETQKLQYCDIQQT
Sbjct: 61  TRKSITTKAGRKVKNNPSSKPSDIRNEILEGSVSTSCTVSINKTEKSETQKLQYCDIQQT 120

Query: 121 IIKNKELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSV 180
             K KEL RPVTVI FDIETTGFSR  DRIVEIA QDLSGGENSTFQTLINPECYIPNS+
Sbjct: 121 FTKKKELTRPVTVIAFDIETTGFSRKKDRIVEIAFQDLSGGENSTFQTLINPECYIPNSL 180

Query: 181 VHGITSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRC 240
           VHGITSRMVSRPDVPRMK+LIPILLQF+ SRQKPDGY+LLVAHNARTFDVPFLL EF RC
Sbjct: 181 VHGITSRMVSRPDVPRMKDLIPILLQFVLSRQKPDGYILLVAHNARTFDVPFLLNEFSRC 240

Query: 241 SVDIPPNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSM 300
           S DIP +WLF DN++LARQLM SSDSK++KISLQALREYY+IPLVGSAHRAMSDV SLS+
Sbjct: 241 SFDIPQDWLFLDNMILARQLMISSDSKLSKISLQALREYYSIPLVGSAHRAMSDVMSLSL 300

Query: 301 ILQRLTFELKLGIPDLVERSFTASDLINKKKN 333
           ILQRLTF+LKL IPDL+ERSFTASDLI K K+
Sbjct: 301 ILQRLTFDLKLEIPDLIERSFTASDLIKKNKD 332

BLAST of Tan0021985.1 vs. ExPASy TrEMBL
Match: A0A0A0LVY3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080 PE=4 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 5.7e-141
Identity = 256/326 (78.53%), Postives = 280/326 (85.89%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRWTRKSV 65
           MC SI R PK+GV  LANLWR NFHPL RN+GH+CWYDQ   RIY+LEGG NKRWTRKS+
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 66  TTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQTIIKNK 125
           TTKAG KAKTNPSSKP+NIRNEIL+E +  SC V+INKSEKSETQKLQYCDIQ TIIK+K
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 126 ELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSVVHGIT 185
           E A  VTVITFDIETTGFSR+LDRIVEIA QDLSGGENSTFQTLINP+CYI NS +HGI+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 186 SRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDIP 245
           +RMV  P VPRM+ELIPI+LQF++SRQKP GYVLLVAHNARTFDVPFLL EF R SVDIP
Sbjct: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 246 PNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQRL 305
            NWLFFD + LARQLMK SDSKITKI+LQAL EYY I L G AHRA+SDV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 306 TFELKLGIPDLVERSFTASDLINKKK 332
           TF+LKL I DLVER+FT  DLINKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKK 326

BLAST of Tan0021985.1 vs. ExPASy TrEMBL
Match: A0A0N9LRH7 (Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.7e-140
Identity = 255/326 (78.22%), Postives = 279/326 (85.58%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRWTRKSV 65
           MC SI R PK+GV  LANLWR NFHPL RN+GH+CWYDQ   RIY+LEGG NKRWTRKS+
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 66  TTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQTIIKNK 125
           TTKAG KAKTNPSSKP+NIRNEIL+E +  SC V+INKSEKSETQKLQYCDIQ TIIK+K
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESVLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 126 ELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECYIPNSVVHGIT 185
           E A  VTVITFDIETTGFSR+LDRIVEIA QDLSGGENSTFQTLINP+CYI NS +HGI+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 186 SRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDIP 245
           +RMV  P VP M+ELIPI+LQF++SRQKP GYVLLVAHNARTFDVPFLL EF R SVDIP
Sbjct: 181 NRMVDSPSVPSMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 246 PNWLFFDNILLARQLMKSSDSKITKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQRL 305
            NWLFFD + LARQLMK SDSKITKI+LQAL EYY I L G AHRA+SDV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 306 TFELKLGIPDLVERSFTASDLINKKK 332
           TF+LKL I DLVER+FT  DLINKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKK 326

BLAST of Tan0021985.1 vs. TAIR 10
Match: AT5G26940.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 4.8e-63
Identity = 138/323 (42.72%), Postives = 202/323 (62.54%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRWTRKSV 65
           MC SI +V +  +H+  +      H   +N   S        R  S++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 66  TTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQTIIKNK 125
           +T       T   S+ SN ++ +L   +  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST-------TTQGSR-SNTKSSVLGGTVPVTRII----DEESRTKVQPFGNLQQRLAQDK 120

Query: 126 ELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECY-IPNSVVHGI 185
           +L++ +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDI 245
            + MV RP+VPRM+ELIPI L+++ SRQKP GYV+LVAHN ++FD  FL+ EF RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PPNWLFFDNILLARQLMKSSDSKI-TKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY++   G AHRA+SDV  LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFELKLGIPDLVERSFTASDL 327
           +LT +LKL + DLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Tan0021985.1 vs. TAIR 10
Match: AT5G26940.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 4.8e-63
Identity = 138/323 (42.72%), Postives = 202/323 (62.54%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRWTRKSV 65
           MC SI +V +  +H+  +      H   +N   S        R  S++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 66  TTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQTIIKNK 125
           +T       T   S+ SN ++ +L   +  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST-------TTQGSR-SNTKSSVLGGTVPVTRII----DEESRTKVQPFGNLQQRLAQDK 120

Query: 126 ELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECY-IPNSVVHGI 185
           +L++ +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDI 245
            + MV RP+VPRM+ELIPI L+++ SRQKP GYV+LVAHN ++FD  FL+ EF RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PPNWLFFDNILLARQLMKSSDSKI-TKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY++   G AHRA+SDV  LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFELKLGIPDLVERSFTASDL 327
           +LT +LKL + DLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Tan0021985.1 vs. TAIR 10
Match: AT5G26940.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 4.8e-63
Identity = 138/323 (42.72%), Postives = 202/323 (62.54%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRWTRKSV 65
           MC SI +V +  +H+  +      H   +N   S        R  S++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 66  TTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQTIIKNK 125
           +T       T   S+ SN ++ +L   +  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST-------TTQGSR-SNTKSSVLGGTVPVTRII----DEESRTKVQPFGNLQQRLAQDK 120

Query: 126 ELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECY-IPNSVVHGI 185
           +L++ +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDI 245
            + MV RP+VPRM+ELIPI L+++ SRQKP GYV+LVAHN ++FD  FL+ EF RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PPNWLFFDNILLARQLMKSSDSKI-TKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY++   G AHRA+SDV  LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFELKLGIPDLVERSFTASDL 327
           +LT +LKL + DLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Tan0021985.1 vs. TAIR 10
Match: AT5G26940.4 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 4.8e-63
Identity = 138/323 (42.72%), Postives = 202/323 (62.54%), Query Frame = 0

Query: 6   MCFSILRVPKFGVHTLANLWRVNFHPLKRNYGHSCWYDQFGSRIYSLEGGPNKRWTRKSV 65
           MC SI +V +  +H+  +      H   +N   S        R  S++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 66  TTKAGRKAKTNPSSKPSNIRNEILEEPISTSCAVSINKSEKSETQKLQYCDIQQTIIKNK 125
           +T       T   S+ SN ++ +L   +  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST-------TTQGSR-SNTKSSVLGGTVPVTRII----DEESRTKVQPFGNLQQRLAQDK 120

Query: 126 ELARPVTVITFDIETTGFSRDLDRIVEIALQDLSGGENSTFQTLINPECY-IPNSVVHGI 185
           +L++ +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL+NP    I N+ +HGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 186 TSRMVSRPDVPRMKELIPILLQFIRSRQKPDGYVLLVAHNARTFDVPFLLKEFKRCSVDI 245
            + MV RP+VPRM+ELIPI L+++ SRQKP GYV+LVAHN ++FD  FL+ EF RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 246 PPNWLFFDNILLARQLMKSSDSKI-TKISLQALREYYNIPLVGSAHRAMSDVWSLSMILQ 305
           P NWL  D++ LAR+ MKS +  +    SL+AL +YY++   G AHRA+SDV  LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 306 RLTFELKLGIPDLVERSFTASDL 327
           +LT +LKL + DLV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q682U66.7e-6242.72Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q895K23.4e-1333.33DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) ... [more]
Q8RA325.4e-1130.16DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongens... [more]
Q8XJR31.2e-1032.37DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=... [more]
A7X1P41.2e-0725.40DNA polymerase III PolC-type OS=Staphylococcus aureus (strain Mu3 / ATCC 700698)... [more]
Match NameE-valueIdentityDescription
KAG6590475.11.6e-16689.46Exonuclease DPD1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma s... [more]
XP_022961373.16.3e-16689.16exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata] >XP_022961374... [more]
XP_023515555.18.2e-16689.16exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022968752.16.9e-16589.43exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1... [more]
XP_038878496.17.9e-16188.32exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP... [more]
Match NameE-valueIdentityDescription
A0A6J1HDU63.0e-16689.16exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1HUD73.3e-16589.43exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1C1V82.3e-15884.64exonuclease DPD1, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=... [more]
A0A0A0LVY35.7e-14178.53Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080... [more]
A0A0N9LRH71.7e-14078.22Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G26940.14.8e-6342.72Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.24.8e-6342.72Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.34.8e-6342.72Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.44.8e-6342.72Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 132..311
e-value: 7.1E-27
score: 105.3
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 135..295
e-value: 5.9E-19
score: 69.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 131..310
e-value: 7.7E-42
score: 145.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..83
NoneNo IPR availablePANTHERPTHR13058:SF23POLYMERASE III POLC-TYPE, PUTATIVE ISOFORM 1-RELATEDcoord: 3..329
NoneNo IPR availableCDDcd06127DEDDhcoord: 136..303
e-value: 1.52899E-39
score: 135.121
IPR040393Three-prime repair exonuclease 1/2PANTHERPTHR13058THREE PRIME REPAIR EXONUCLEASE 1, 2coord: 3..329
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 129..308

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Tan0021985Tan0021985gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0021985.1-five_prime_utrTan0021985.1-five_prime_utr-LG09:5227373..5227534five_prime_UTR
Tan0021985.1-five_prime_utrTan0021985.1-five_prime_utr-LG09:5228501..5228578five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0021985.1-exonTan0021985.1-exon-LG09:5227373..5227534exon
Tan0021985.1-exonTan0021985.1-exon-LG09:5228501..5229165exon
Tan0021985.1-exonTan0021985.1-exon-LG09:5229343..5229548exon
Tan0021985.1-exonTan0021985.1-exon-LG09:5229648..5230108exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0021985.1-cdsTan0021985.1-cds-LG09:5228579..5229165CDS
Tan0021985.1-cdsTan0021985.1-cds-LG09:5229343..5229548CDS
Tan0021985.1-cdsTan0021985.1-cds-LG09:5229648..5229853CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0021985.1-three_prime_utrTan0021985.1-three_prime_utr-LG09:5229854..5230108three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Tan0021985.1Tan0021985.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000738 DNA catabolic process, exonucleolytic
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008296 3'-5'-exodeoxyribonuclease activity