Csor.00g301250 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g301250
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCsor_Chr18: 10400924 .. 10403075 (+)
RNA-Seq ExpressionCsor.00g301250
SyntenyCsor.00g301250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATCTGCAAATCCTTATTTTGATGACGTTCGGACGAAACCGGAGGTAATCGATCCTCCCCAAGATGAGGACATGATGGATGTTAGCGAAAGTGTTAGTGATCCTACTCGAAATGGAGGAAAGCCTAATGTAGCTGTCTCTAGCAGCGTTCGTGAGCTGTTGGAGTGTCCTGTTTGCTTGAATGCTATGTACCCTCCCATTCATCAGGTCTGTTGCCATCTTTTCAAAATCTTTTGAACGAAATTGCTTACTTTTTTTAAATTCCTTTAATCTAATTTGTCGACACGATATTGGCCTTTTTCTTAAAGGTGTCTAAGGAAGGTACCATATAGGTGAACGTATTCAATAGTTTAAACGAACAACTAGAAGCCATACTGTACCTTGGAAGATAGATGATTTTGTGATAAGGAATCGCTACAAAAGAAGTAAGATTTGGATTATCTTATTGATCAAATATCACAAGCAACATTGATCATGTCAAGCTTGAATGATTCTAAACATGCAATCTAAACTACGAAGAATTGAAAAAAAAAAAAAAAAGAACTTAGCCCTCGGATAAAGAAAAGGTAAGAACTGCGAGATCTCTAGTGAGGACACTGGGCCCCGAAGTGGGGTTGATTGTGAGATCCCACATCGGATGGAGAGGGGAATAAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACACGTTTTAATGACCTTGAGGGGAACCCGAAAGGGAAAGCCCGAAGAGGACAATATCTGATAGTGGTGGGCTTGGGCTATTACAAAAACACTTAGCTCTGATGATGGAAACTAAAATTCGGATTACCTTGTTGATCAAATATCTCAAGGTAAGAACACTTGTTTGAGATACAAATCACTCCACAAACAAGATCGATCATGTCAAGCTTGAATGATTCTAAACATGCAATCTTAATTACGTAGAATTGCAAAGAAACTTAGTCATTGGCTAAAGGAAAGCACAAATGCTCTTTTTACTCGTCCCTTTCACAAAGACAACATACACCGCTCTATATAGCTTCAAAATAAAAACCTTAACCTTAGGCATTCCAAGAGCCATAACTTTCATAGTATAGCAGGTTAGACGTCTGATCTGGATGATATCTACCGTGTTAATACCACCTAAAATCAACGTTCAAATCTTACTTTCTTAATATAGTTGTGCTTTGCATGGATGTAGTCTGCTACTTACTCAGAACCTTCATTTTGGCATTTGAGCTTGCCTTGGTTCATATCGATTAACGTTCTGTCATTGCAGTGTTCGAATGGTCACACATTGTGTTCGGGTTGCAAGCCTAGGGTGCACAACCGGTGCCCGACCTGCAGGCATGAACTTGGCAATATCAGATGTCTTGCATTAGAGAAAGTGGCTGCATCTTTGGAACTTCCATGTACATATCAAACCTTTGGGTGCGCGGGCATATACCCGTACTATAGCAAGCTGAAACATGAATCTCAATGTGCATATCGACCCTATAACTGTCCGTATGCGGGGTCGGAATGCACAGTTGTTGGTGATATTCCATTTTTGGTAGCACACCTAAAGGATGATCACAAAGTCGACATGCATAGTGGGAGCACTTTCAATCATCGATACGTCAAGTCAAACCCGCAAGAGGTCGAAAATGCTACCTGGATGCTAACGGTACTATTCTACTTCATATAAATCATTTTTGGGTTAAACATGTATTCAAATGAAGCCTTCGTTATCAAGAACGAGCGTATTAGCTAGAAGAGGCTAACGAATCGAGTTCATAGGTTGTAAATATTGAGATGTCTGATACAAAACTGATACCGTTTTTTCGGTTGACTTGTAGGTTTTCAGCTGTTTTGGTCATTATTTTTGTTTACATTTCGAAGCTTTCCAGCTCGGAATGGCACCTGTCTATATAGCCTTCTTGCGCTTCATGGGTGACGATAACGAGGCAAAGAACTACAGTTATAGCCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTTTGGCAAGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTGCGAGACAGCTTTGACGGGCTTATTATCCAACGAAACATGGCTCTGTTCTTCTCGGGCGGAGATCGGAAGGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTGA

mRNA sequence

ATGTCATCTGCAAATCCTTATTTTGATGACGTTCGGACGAAACCGGAGGTAATCGATCCTCCCCAAGATGAGGACATGATGGATGTTAGCGAAAGTGTTAGTGATCCTACTCGAAATGGAGGAAAGCCTAATGTAGCTGTCTCTAGCAGCGTTCGTGAGCTGTTGGAGTGTCCTGTTTGCTTGAATGCTATGTACCCTCCCATTCATCAGTGTTCGAATGGTCACACATTGTGTTCGGGTTGCAAGCCTAGGGTGCACAACCGGTGCCCGACCTGCAGGCATGAACTTGGCAATATCAGATGTCTTGCATTAGAGAAAGTGGCTGCATCTTTGGAACTTCCATGTACATATCAAACCTTTGGGTGCGCGGGCATATACCCGTACTATAGCAAGCTGAAACATGAATCTCAATGTGCATATCGACCCTATAACTGTCCGTATGCGGGGTCGGAATGCACAGTTGTTGGTGATATTCCATTTTTGGTAGCACACCTAAAGGATGATCACAAAGTCGACATGCATAGTGGGAGCACTTTCAATCATCGATACGTCAAGTCAAACCCGCAAGAGGTCGAAAATGCTACCTGGATGCTAACGGTTTTCAGCTGTTTTGGTCATTATTTTTGTTTACATTTCGAAGCTTTCCAGCTCGGAATGGCACCTGTCTATATAGCCTTCTTGCGCTTCATGGGTGACGATAACGAGGCAAAGAACTACAGTTATAGCCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTTTGGCAAGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTGCGAGACAGCTTTGACGGGCTTATTATCCAACGAAACATGGCTCTGTTCTTCTCGGGCGGAGATCGGAAGGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTGA

Coding sequence (CDS)

ATGTCATCTGCAAATCCTTATTTTGATGACGTTCGGACGAAACCGGAGGTAATCGATCCTCCCCAAGATGAGGACATGATGGATGTTAGCGAAAGTGTTAGTGATCCTACTCGAAATGGAGGAAAGCCTAATGTAGCTGTCTCTAGCAGCGTTCGTGAGCTGTTGGAGTGTCCTGTTTGCTTGAATGCTATGTACCCTCCCATTCATCAGTGTTCGAATGGTCACACATTGTGTTCGGGTTGCAAGCCTAGGGTGCACAACCGGTGCCCGACCTGCAGGCATGAACTTGGCAATATCAGATGTCTTGCATTAGAGAAAGTGGCTGCATCTTTGGAACTTCCATGTACATATCAAACCTTTGGGTGCGCGGGCATATACCCGTACTATAGCAAGCTGAAACATGAATCTCAATGTGCATATCGACCCTATAACTGTCCGTATGCGGGGTCGGAATGCACAGTTGTTGGTGATATTCCATTTTTGGTAGCACACCTAAAGGATGATCACAAAGTCGACATGCATAGTGGGAGCACTTTCAATCATCGATACGTCAAGTCAAACCCGCAAGAGGTCGAAAATGCTACCTGGATGCTAACGGTTTTCAGCTGTTTTGGTCATTATTTTTGTTTACATTTCGAAGCTTTCCAGCTCGGAATGGCACCTGTCTATATAGCCTTCTTGCGCTTCATGGGTGACGATAACGAGGCAAAGAACTACAGTTATAGCCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTTTGGCAAGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTGCGAGACAGCTTTGACGGGCTTATTATCCAACGAAACATGGCTCTGTTCTTCTCGGGCGGAGATCGGAAGGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTGA

Protein sequence

MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ
Homology
BLAST of Csor.00g301250 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 1.9e-156
Identity = 259/299 (86.62%), Postives = 272/299 (90.97%), Query Frame = 0

Query: 7   YFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVCLNAMYP 66
           Y DD  +  EVIDPP+ E ++DV+E   DP  +  KPNV VSSSVRELLECPVCL+AMYP
Sbjct: 6   YIDD--SGSEVIDPPKTE-VLDVTELAGDPVPHSPKPNVVVSSSVRELLECPVCLSAMYP 65

Query: 67  PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTFGCAGIY 126
           PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPC YQ FGC GIY
Sbjct: 66  PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQNFGCVGIY 125

Query: 127 PYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFNHRYVKS 186
           PYY KLKHESQC YRPY+CPYAGSECTV GDIP+LV HLKDDHKVDMH+G TFNHRYVKS
Sbjct: 126 PYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDDHKVDMHNGCTFNHRYVKS 185

Query: 187 NPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVG 246
           NP EVENATWMLTVFSCFG YFCLHFEAFQLGMAPVYIAFLRFMGDD EAKNYSYSLEVG
Sbjct: 186 NPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDLEAKNYSYSLEVG 245

Query: 247 GNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           G GRKM+WQGVPRSIRDSHRKVRDS+DGLIIQRNMALFFSGG+RKELKLRVTGRIWKEQ
Sbjct: 246 GTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGGERKELKLRVTGRIWKEQ 301

BLAST of Csor.00g301250 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 482.6 bits (1241), Expect = 3.2e-135
Identity = 223/289 (77.16%), Postives = 246/289 (85.12%), Query Frame = 0

Query: 18  IDPPQDEDMMDVSESVSDPTRNGGKPNV-AVSSSVRELLECPVCLNAMYPPIHQCSNGHT 77
           ID   D+D +      S     GG   V + ++SV ELLECPVC N+MYPPIHQC NGHT
Sbjct: 6   IDSVIDDDEIHQKHQFSSTKSQGGATVVISPATSVYELLECPVCTNSMYPPIHQCHNGHT 65

Query: 78  LCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHES 137
           LCS CK RVHNRCPTCR ELG+IRCLALEKVA SLELPC Y   GC GI+PYYSKLKHES
Sbjct: 66  LCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYSKLKHES 125

Query: 138 QCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATW 197
           QC +RPY+CPYAGSEC  VGDI FLVAHL+DDHKVDMH+G TFNHRYVKSNP+EVENATW
Sbjct: 126 QCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATW 185

Query: 198 MLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQG 257
           MLTVF CFG YFCLHFEAFQLGMAPVY+AFLRFMGD+++A+NY+YSLEVGG+GRK  W+G
Sbjct: 186 MLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEG 245

Query: 258 VPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
            PRS+RDSHRKVRDS DGLIIQRNMALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 246 TPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQ 294

BLAST of Csor.00g301250 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 1.2e-134
Identity = 215/258 (83.33%), Postives = 236/258 (91.47%), Query Frame = 0

Query: 48  SSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 107
           ++SV ELLECPVC N+MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 54  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 113

Query: 108 AASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKD 167
           A SLELPC + + GC  I+PYYSKLKHE+ C +RPY+CPYAGSEC+V GDIPFLVAHL+D
Sbjct: 114 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 173

Query: 168 DHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFL 227
           DHKVDMHSG TFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGMAPVY+AFL
Sbjct: 174 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 233

Query: 228 RFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSG 287
           RFMGD+ EA+NY+YSLEVGG GRK++W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSG
Sbjct: 234 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 293

Query: 288 GDRKELKLRVTGRIWKEQ 306
           GDRKELKLRVTGRIWKEQ
Sbjct: 294 GDRKELKLRVTGRIWKEQ 311

BLAST of Csor.00g301250 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 1.1e-132
Identity = 215/265 (81.13%), Postives = 236/265 (89.06%), Query Frame = 0

Query: 41  GKPNVAVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIR 100
           G    A ++SV ELLECPVC  +MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IR
Sbjct: 48  GPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIR 107

Query: 101 CLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPF 160
           CLALEKVA SLELPC +   GC  I+PYYSKLKHES C +RPY+CPYAGSEC +VGDIPF
Sbjct: 108 CLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPF 167

Query: 161 LVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMA 220
           LVAHL+DDHKVDMH+GSTFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGM 
Sbjct: 168 LVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMG 227

Query: 221 PVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRN 280
           PVY+AFLRFMGD+ +A++YSYSLEVGG+GRK+ W+G PRSIRDSHRKVRDS DGLIIQRN
Sbjct: 228 PVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRN 287

Query: 281 MALFFSGGDRKELKLRVTGRIWKEQ 306
           MALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 288 MALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of Csor.00g301250 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 2.4e-130
Identity = 213/274 (77.74%), Postives = 233/274 (85.04%), Query Frame = 0

Query: 36  PTRNG----GKPNVAVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPT 95
           PT+ G    GK  +  ++ V ELLECPVC N MYPPIHQC NGHTLCS CK RV N CPT
Sbjct: 35  PTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPT 94

Query: 96  CRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSE 155
           CR+ELGNIRCLALEKVA SLE+PC YQ  GC  I+PYYSKLKHE  C +RPY CPYAGSE
Sbjct: 95  CRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSE 154

Query: 156 CTVVGDIPFLVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLH 215
           C+V GDIP LV HLKDDHKVDMH G TFNHRYVKSNP EVENATWMLTVF+CFG  FCLH
Sbjct: 155 CSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLH 214

Query: 216 FEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDS 275
           FEAFQLGMAPVY+AFLRFMGD+NEAK +SYSLEVG +GRK+ WQG+PRSIRDSHRKVRDS
Sbjct: 215 FEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDS 274

Query: 276 FDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
            DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 275 QDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of Csor.00g301250 vs. NCBI nr
Match: XP_022945607.1 (E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita moschata] >KAG6573809.1 E3 ubiquitin-protein ligase DIS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 646 bits (1667), Expect = 1.61e-234
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Csor.00g301250 vs. NCBI nr
Match: XP_022945606.1 (E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 646 bits (1667), Expect = 2.44e-234
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 12  MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 71

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 72  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 131

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN
Sbjct: 132 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 191

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 192 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 251

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 252 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 311

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 312 IWKEQ 316

BLAST of Csor.00g301250 vs. NCBI nr
Match: XP_023541838.1 (E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645 bits (1664), Expect = 4.63e-234
Identity = 304/305 (99.67%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMH+GSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Csor.00g301250 vs. NCBI nr
Match: XP_023541837.1 (E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645 bits (1664), Expect = 6.99e-234
Identity = 304/305 (99.67%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 12  MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 71

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 72  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 131

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMH+GSTFN
Sbjct: 132 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 191

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 192 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 251

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 252 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 311

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 312 IWKEQ 316

BLAST of Csor.00g301250 vs. NCBI nr
Match: KAG7012878.1 (E3 ubiquitin-protein ligase SINAT5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 644 bits (1660), Expect = 1.89e-233
Identity = 303/305 (99.34%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDEDMMDVS+SVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSKSVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMH+GSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Csor.00g301250 vs. ExPASy TrEMBL
Match: A0A6J1G1E2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449792 PE=3 SV=1)

HSP 1 Score: 646 bits (1667), Expect = 7.82e-235
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Csor.00g301250 vs. ExPASy TrEMBL
Match: A0A6J1G1D3 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449792 PE=3 SV=1)

HSP 1 Score: 646 bits (1667), Expect = 1.18e-234
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 12  MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 71

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 72  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 131

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN
Sbjct: 132 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 191

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 192 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 251

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 252 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 311

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 312 IWKEQ 316

BLAST of Csor.00g301250 vs. ExPASy TrEMBL
Match: A0A6J1HPK3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466544 PE=3 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 5.92e-230
Identity = 298/305 (97.70%), Postives = 300/305 (98.36%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MS ANPYF+DVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LN MYPPIH CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSEC VVGDIPFLVAHLKDDHKVDMH+GSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNE KNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Csor.00g301250 vs. ExPASy TrEMBL
Match: A0A6J1HQW6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466544 PE=3 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 8.94e-230
Identity = 298/305 (97.70%), Postives = 300/305 (98.36%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MS ANPYF+DVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 12  MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 71

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LN MYPPIH CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 72  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 131

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSEC VVGDIPFLVAHLKDDHKVDMH+GSTFN
Sbjct: 132 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 191

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNE KNYS
Sbjct: 192 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 251

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 252 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 311

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 312 IWKEQ 316

BLAST of Csor.00g301250 vs. ExPASy TrEMBL
Match: A0A6J1GX87 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111458334 PE=3 SV=1)

HSP 1 Score: 629 bits (1621), Expect = 8.08e-228
Identity = 296/305 (97.05%), Postives = 298/305 (97.70%), Query Frame = 0

Query: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQD+DMMDVSESVSDP RNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPC Y TF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYLTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180
           GC GIYPYYSKLKHESQC YRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMH+GSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFG YFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK VWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 305
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Csor.00g301250 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 480.7 bits (1236), Expect = 8.6e-136
Identity = 215/258 (83.33%), Postives = 236/258 (91.47%), Query Frame = 0

Query: 48  SSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 107
           ++SV ELLECPVC N+MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 54  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 113

Query: 108 AASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKD 167
           A SLELPC + + GC  I+PYYSKLKHE+ C +RPY+CPYAGSEC+V GDIPFLVAHL+D
Sbjct: 114 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 173

Query: 168 DHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFL 227
           DHKVDMHSG TFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGMAPVY+AFL
Sbjct: 174 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 233

Query: 228 RFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSG 287
           RFMGD+ EA+NY+YSLEVGG GRK++W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSG
Sbjct: 234 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 293

Query: 288 GDRKELKLRVTGRIWKEQ 306
           GDRKELKLRVTGRIWKEQ
Sbjct: 294 GDRKELKLRVTGRIWKEQ 311

BLAST of Csor.00g301250 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 474.2 bits (1219), Expect = 8.1e-134
Identity = 215/265 (81.13%), Postives = 236/265 (89.06%), Query Frame = 0

Query: 41  GKPNVAVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIR 100
           G    A ++SV ELLECPVC  +MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IR
Sbjct: 48  GPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIR 107

Query: 101 CLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPF 160
           CLALEKVA SLELPC +   GC  I+PYYSKLKHES C +RPY+CPYAGSEC +VGDIPF
Sbjct: 108 CLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPF 167

Query: 161 LVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMA 220
           LVAHL+DDHKVDMH+GSTFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGM 
Sbjct: 168 LVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMG 227

Query: 221 PVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRN 280
           PVY+AFLRFMGD+ +A++YSYSLEVGG+GRK+ W+G PRSIRDSHRKVRDS DGLIIQRN
Sbjct: 228 PVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRN 287

Query: 281 MALFFSGGDRKELKLRVTGRIWKEQ 306
           MALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 288 MALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of Csor.00g301250 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 466.5 bits (1199), Expect = 1.7e-131
Identity = 213/274 (77.74%), Postives = 233/274 (85.04%), Query Frame = 0

Query: 36  PTRNG----GKPNVAVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPT 95
           PT+ G    GK  +  ++ V ELLECPVC N MYPPIHQC NGHTLCS CK RV N CPT
Sbjct: 35  PTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPT 94

Query: 96  CRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSE 155
           CR+ELGNIRCLALEKVA SLE+PC YQ  GC  I+PYYSKLKHE  C +RPY CPYAGSE
Sbjct: 95  CRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSE 154

Query: 156 CTVVGDIPFLVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLH 215
           C+V GDIP LV HLKDDHKVDMH G TFNHRYVKSNP EVENATWMLTVF+CFG  FCLH
Sbjct: 155 CSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLH 214

Query: 216 FEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDS 275
           FEAFQLGMAPVY+AFLRFMGD+NEAK +SYSLEVG +GRK+ WQG+PRSIRDSHRKVRDS
Sbjct: 215 FEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDS 274

Query: 276 FDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
            DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 275 QDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of Csor.00g301250 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 456.8 bits (1174), Expect = 1.3e-128
Identity = 210/277 (75.81%), Postives = 232/277 (83.75%), Query Frame = 0

Query: 31  ESVSDPTRNGGKP--NVAVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNR 90
           E+ S PT++G         S+ V ELLECPVC N MYPPIHQC NGHTLCS CK RV N 
Sbjct: 29  EANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNT 88

Query: 91  CPTCRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYA 150
           CPTCR+ELGNIRCLALEKVA SLE+PC YQ  GC  I+PYYSKLKHE  C +R Y+CPYA
Sbjct: 89  CPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYA 148

Query: 151 GSECTVVGDIPFLVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYF 210
           GSEC+V GDIP LV HLKDDHKVDMH G TFNHRYVKSNP EVENATWMLTVF+CFG  F
Sbjct: 149 GSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQF 208

Query: 211 CLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKV 270
           CLHFEAFQLGMAPVY+AFLRFMGD+NEAK +SYSLEVG + RK+ WQG+PRSIRDSHRKV
Sbjct: 209 CLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKV 268

Query: 271 RDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           RDS DGLII RN+AL+FSG D++ELKLRVTGRIWKE+
Sbjct: 269 RDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

BLAST of Csor.00g301250 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 4.6e-105
Identity = 170/204 (83.33%), Postives = 187/204 (91.67%), Query Frame = 0

Query: 102 LALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFL 161
           +ALEKVA SLELPC Y   GC GI+PYYSKLKHESQC +RPY+CPYAGSEC  VGDI FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 162 VAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAP 221
           VAHL+DDHKVDMH+G TFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 222 VYIAFLRFMGDDNEAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNM 281
           VY+AFLRFMGD+++A+NY+YSLEVGG+GRK  W+G PRS+RDSHRKVRDS DGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 282 ALFFSGGDRKELKLRVTGRIWKEQ 306
           ALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q10L911.9e-15686.62E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q8S3N13.2e-13577.16E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q84JL31.2e-13483.33E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN81.1e-13281.13E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q9M2P42.4e-13077.74E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
Match NameE-valueIdentityDescription
XP_022945607.11.61e-234100.00E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita moschata] >KAG657380... [more]
XP_022945606.12.44e-234100.00E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita moschata][more]
XP_023541838.14.63e-23499.67E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023541837.16.99e-23499.67E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7012878.11.89e-23399.34E3 ubiquitin-protein ligase SINAT5 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1G1E27.82e-235100.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449792... [more]
A0A6J1G1D31.18e-234100.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449792... [more]
A0A6J1HPK35.92e-23097.70RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466544 P... [more]
A0A6J1HQW68.94e-23097.70RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466544 P... [more]
A0A6J1GX878.08e-22897.05RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111458334... [more]
Match NameE-valueIdentityDescription
AT3G61790.18.6e-13683.33Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.18.1e-13481.13Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.11.7e-13177.74seven in absentia of Arabidopsis 2 [more]
AT2G41980.11.3e-12875.81Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.14.6e-10583.33TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 110..164
e-value: 1.3E-15
score: 59.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 18..109
e-value: 4.8E-9
score: 37.8
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 165..298
e-value: 3.9E-39
score: 135.4
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 99..298
e-value: 5.2E-80
score: 268.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..45
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 7..305
NoneNo IPR availablePANTHERPTHR10315:SF77E3 UBIQUITIN-PROTEIN LIGASE DIS1coord: 7..305
NoneNo IPR availableCDDcd03829Sinacoord: 171..299
e-value: 2.33986E-65
score: 199.535
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 55..93
e-value: 3.37387E-15
score: 66.1542
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 46..99
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 110..302
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 57..93
score: 9.346889
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 110..170
score: 13.852739

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g301250.m01Csor.00g301250.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding