Csor.00g086280 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g086280
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionMethyltransferase-like protein
LocationCsor_Chr05: 9980226 .. 9983127 (-)
RNA-Seq ExpressionCsor.00g086280
SyntenyCsor.00g086280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGAAGACGCCGAATATTTCTCCAAAGATTTCGACTGGGAAGAGCTACGAATTCGGGTCGAGAACGACCCGCTTTTGCAGTACCATTTGCTTCCATTTCAGCCCTGCGATTCGATTCCCTCAGTTTCTGCAGAAGCCGATTCAGACGCTTGGAGCCGCTTTCACCTCCGCCACTCCTCCGGCAAATTCTTCAAGGTTTTGATTCCTTTTAGCGTATTCGTAGCAACCTAACTTTGTTGTTTCTTTTAGATTAGTTGTGATTTGAGCTCTTTGCGTTTCGCTTCAGGAAAGGCGGTATTTGTTGAAGGAATTCCCTGAACTGGTTTCCTGTAAGAAGAACTGTAAAGTTTTGGAGGTTGGTTGTGGTAATGGAAGCACTGCTCTTCCAATCTTGCGGTAACCTCCAACGTATTGAACTTTTCCTCTGCTTTACTTCTTATTGATGTTCACCAATTTTTTGGGAGTTTGATAACGTAGAAATTTGGTTGATACAAACTAGTTGCATCGTCTTTGTTTATATGAAGTACTGGCCAATTCCTCCATGTTGTATGTTATAGGATTGATAGGTAGCATCTTTGGAAGATGAATATAATCTATATCTCTCCTTTTTTAGGGTTCTTCACATAACCTCTTAATGTGTTCCGTTCTCGATTAGTTACGAGTGAGAGGTGCCCATTGTAAGAGCGAATTGATCCTTCACTTCCTTTTGAGATCTTGATTCTGGACTATCCATCCTACTCTATGCAGGTTCTCATTTGCATCATCGGTAGGATATACGCTCATATAACGATATACTCTTCCATATTCCGTTGAAATTCGAAACTTTTCATGTAGGTTGATATTCATATACTTCGTCGATAAAGTTAGAGCTTATAGAAAACAATGATAATTGATTGGCCAAGTTGAGACATTTAGTGAGGGCCTATGGAAAATGACCTTTTGGTTTTTAACACCGAAACCACTTAGAACGGAGTATGGTCGCTTCATTTTCCTCATCTCTCCCCTTCTAAATGAAGGATTGTATACATTTTTCTAACTTTGTTCAAGAGCTGAATCACAGATATTCCTTCATGAAACTTTATTAGGTGTTTTGAGTGCATCTGCGAAGATTATGGAATCGAACACAGCGTTCACAATGTTTTATGTTCATCCCAGTACTTGTAGTGCTTTTGCATGTTCAAGATAGAAGAAATTTGTACCATTAAATATGGCCTTTGAGTGGTAGATCTTATTGCTACCAATTCATTATCTTTACACATGATTACTTTCTTTGTAGTGGGAACGAAAATATTGTCATCTATGCTTGCGATTGTAGCACGGAAACTCTTGAGAGAGCTAAAGAGATTATAAATGATGTCGGTATTGCATCTTTAAAAGATCGATTCTGTCCATTCTATTGTGATTTTTCTACTAGTAAGTTTCCAACATGGTTGGCCTGCAATTCATGCCGTGGAAACACTTCGCTACAGCAGCCGAGTTTTACTACTCCAGGTTGTTATTTTGCCTACCCCTTTAGACATAACTCATTATGTGATGTCCCACGTTAGTTGGGGAGGAGAACAAACCACCATTTATAAGGGTGTGGAAACCTTCCCCTAGCAGACGCGTTTTAAAACCTTGAGGGAAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCTAACGGTGGATCTGGGTCGTTACACATCAACTGCAAGACTACTGCTTTCCTTACAGCCTTGCACCCTTATCCATCTTTCTCTATGTTTGCAGAGAAGGAAGGATCCCAGGCCACCGAATCAGAAGAAAGTGAATGTTGCATTGGTGGGGTTGATTTTCTTACTTTGGTATGCTCCTCTTTTTGTATTTTTATATAGTTACTCTAATTTTATCCAATGCATTGGTTTAATATATATATATATATATATATATATATATACACACACTTGGTGGAGCATTTTATATTTGCAAAGAAATTAATGAAATATCTAAATGGTGTTAAGGCATGAACGCTCTCTCCCTTGGTGAAAACTAATACATTGTGCAACATTAACAAACTTCCTTCACTTCTAATGATTTCTTGTGTGATGTCCCACTGACAAACCCTAAATGGTGTGGAAACCTTTCTCTAGCAGACGTGTTTCAAAGCCTTAAGGGGAAGCCCGAAAGGGAAAACCCAAAAAGGACAATATCTGCTAGTGGTGGATTTGGGCCGTTACATCTTGAATCTTGGTAATGACAGTTGAATGTTCTTTGGCCTTTTTTACACTATTTCAGATATTTACACTATCAGCAGTGCCTCTCCAGAGGATGCCAGCATCGATTAGAGAATGCTATATGGCGTTGAAGCCTGGAGGCCTGCTCTTATTTCGCGACTACGGTAATAGATTATGATTAGATTGATGATGTTATTTTAGTTTGCTTTAAATCCGAAGTATAGAAAATTCCTGTCCTTTCTAGCTCATGTAACGTGTTTTAAACATTCTGCTGATCGATATCCTGTTTAACGGAATGCAGGTCTATATGATATGACGATGCTTCGATTCGGGCAAGATCAAAGAGTAGGATTCAGGGAATATGTCCGATTGGATGGAACGCGTTCTTATTTCTTCTGCTTAAATACCTTGAGAGATCTGTTTGTCACTGCAGGCTTCGTTGAGGTACTTACTACCAGGTTTAAATACATATACTTCATTATTATAGTTCATATCCCAAACTGTTTACAACAATGGTGATGTCCTGTTACTGACCTTATAAAGTATAGAAAATAATTGATACCGTCCCGACTAACTTTAGAGCATGATTATCGTTGCAGCTCGAGTTGGAATATTGTTGTGTGAAGTCCTTGAATCGCCGAAATGGTAAGAGAATGGAGAGGGTGTGGGTTCATGGAAAATTTCAGAAGCCTGTGTCATGA

mRNA sequence

ATGGGGGAAGACGCCGAATATTTCTCCAAAGATTTCGACTGGGAAGAGCTACGAATTCGGGTCGAGAACGACCCGCTTTTGCAGTACCATTTGCTTCCATTTCAGCCCTGCGATTCGATTCCCTCAGTTTCTGCAGAAGCCGATTCAGACGCTTGGAGCCGCTTTCACCTCCGCCACTCCTCCGGCAAATTCTTCAAGGAAAGGCGGTATTTGTTGAAGGAATTCCCTGAACTGGTTTCCTGTAAGAAGAACTGTAAAGTTTTGGAGGTTGGTTGTGGTAATGGAAGCACTGCTCTTCCAATCTTGCGTGGGAACGAAAATATTGTCATCTATGCTTGCGATTGTAGCACGGAAACTCTTGAGAGAGCTAAAGAGATTATAAATGATGTCGGTATTGCATCTTTAAAAGATCGATTCTGTCCATTCTATTGTGATTTTTCTACTAGTAAGTTTCCAACATGGTTGGCCTGCAATTCATGCCGTGGAAACACTTCGCTACAGCAGCCGAGTTTTACTACTCCAGAGAAGGAAGGATCCCAGGCCACCGAATCAGAAGAAAGTGAATGTTGCATTGGTGGGGTTGATTTTCTTACTTTGATATTTACACTATCAGCAGTGCCTCTCCAGAGGATGCCAGCATCGATTAGAGAATGCTATATGGCGTTGAAGCCTGGAGGCCTGCTCTTATTTCGCGACTACGGTCTATATGATATGACGATGCTTCGATTCGGGCAAGATCAAAGAGTAGGATTCAGGGAATATGTCCGATTGGATGGAACGCGTTCTTATTTCTTCTGCTTAAATACCTTGAGAGATCTGTTTGTCACTGCAGGCTTCGTTGAGCTCGAGTTGGAATATTGTTGTGTGAAGTCCTTGAATCGCCGAAATGGTAAGAGAATGGAGAGGGTGTGGGTTCATGGAAAATTTCAGAAGCCTGTGTCATGA

Coding sequence (CDS)

ATGGGGGAAGACGCCGAATATTTCTCCAAAGATTTCGACTGGGAAGAGCTACGAATTCGGGTCGAGAACGACCCGCTTTTGCAGTACCATTTGCTTCCATTTCAGCCCTGCGATTCGATTCCCTCAGTTTCTGCAGAAGCCGATTCAGACGCTTGGAGCCGCTTTCACCTCCGCCACTCCTCCGGCAAATTCTTCAAGGAAAGGCGGTATTTGTTGAAGGAATTCCCTGAACTGGTTTCCTGTAAGAAGAACTGTAAAGTTTTGGAGGTTGGTTGTGGTAATGGAAGCACTGCTCTTCCAATCTTGCGTGGGAACGAAAATATTGTCATCTATGCTTGCGATTGTAGCACGGAAACTCTTGAGAGAGCTAAAGAGATTATAAATGATGTCGGTATTGCATCTTTAAAAGATCGATTCTGTCCATTCTATTGTGATTTTTCTACTAGTAAGTTTCCAACATGGTTGGCCTGCAATTCATGCCGTGGAAACACTTCGCTACAGCAGCCGAGTTTTACTACTCCAGAGAAGGAAGGATCCCAGGCCACCGAATCAGAAGAAAGTGAATGTTGCATTGGTGGGGTTGATTTTCTTACTTTGATATTTACACTATCAGCAGTGCCTCTCCAGAGGATGCCAGCATCGATTAGAGAATGCTATATGGCGTTGAAGCCTGGAGGCCTGCTCTTATTTCGCGACTACGGTCTATATGATATGACGATGCTTCGATTCGGGCAAGATCAAAGAGTAGGATTCAGGGAATATGTCCGATTGGATGGAACGCGTTCTTATTTCTTCTGCTTAAATACCTTGAGAGATCTGTTTGTCACTGCAGGCTTCGTTGAGCTCGAGTTGGAATATTGTTGTGTGAAGTCCTTGAATCGCCGAAATGGTAAGAGAATGGAGAGGGTGTGGGTTCATGGAAAATTTCAGAAGCCTGTGTCATGA

Protein sequence

MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHSSGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETLERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRMERVWVHGKFQKPVS
Homology
BLAST of Csor.00g086280 vs. ExPASy Swiss-Prot
Match: Q6AXU8 (tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Rattus norvegicus OX=10116 GN=Mettl6 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 94/267 (35.21%), Postives = 131/267 (49.06%), Query Frame = 0

Query: 52  WSRFHLRHSSGKFFKERRYLLKEFPELVSCK----KNCKVLEVGCGNGSTALPILRGNEN 111
           W  F+ R+S+  FFK+R +  +EF EL SC+    +   +LE GCG G+   P+L  + N
Sbjct: 45  WDLFYKRNST-NFFKDRHWTTREFEELRSCREYEGQKLTLLEAGCGVGNCLFPLLEEDSN 104

Query: 112 IVIYACDCSTETLERAKE--IINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTS 171
           I  YACD S   ++  K+  + N        +R   F CD +       +          
Sbjct: 105 IFAYACDFSPRAVDYVKQHPLYN-------AERCKVFQCDLTRDDLLDHI---------- 164

Query: 172 LQQPSFTTPEKEGSQATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPG 231
                   PE                  VD +TLIF LSAV  ++M   +   Y  LKPG
Sbjct: 165 -------PPE-----------------SVDAVTLIFVLSAVHPEKMHLVLLNVYKVLKPG 224

Query: 232 GLLLFRDYGLYDMTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELE 291
             +LFRDYGL D  MLRF    ++G   YVR DGTRSYFF    L  LFV AG+ E+  E
Sbjct: 225 RSVLFRDYGLNDHAMLRFKAGSKLGENFYVRQDGTRSYFFTDEFLAKLFVDAGYEEVVNE 269

Query: 292 YCCVKSLNRRNGKRMERVWVHGKFQKP 313
           Y   +++N++ G  + RV++  KF+KP
Sbjct: 285 YVFRETVNKKEGLCVPRVFLQSKFRKP 269

BLAST of Csor.00g086280 vs. ExPASy Swiss-Prot
Match: Q8TCB7 (tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Homo sapiens OX=9606 GN=METTL6 PE=1 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-32
Identity = 94/266 (35.34%), Postives = 129/266 (48.50%), Query Frame = 0

Query: 52  WSRFHLRHSSGKFFKERRYLLKEFPELVSCK----KNCKVLEVGCGNGSTALPILRGNEN 111
           W  F+ R+S+  FFK+R +  +EF EL SC+    +   +LE GCG G+   P+L  + N
Sbjct: 45  WDLFYKRNST-NFFKDRHWTTREFEELRSCREFEDQKLTMLEAGCGVGNCLFPLLEEDPN 104

Query: 112 IVIYACDCSTETLERAKE-IINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSL 171
           I  YACD S   +E  K+  + D       +R   F CD                  T  
Sbjct: 105 IFAYACDFSPRAIEYVKQNPLYDT------ERCKVFQCDL-----------------TKD 164

Query: 172 QQPSFTTPEKEGSQATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGG 231
                  PE                  VD + LIF LSAV   +M   ++  Y  LKPG 
Sbjct: 165 DLLDHVPPE-----------------SVDVVMLIFVLSAVHPDKMHLVLQNIYKVLKPGK 224

Query: 232 LLLFRDYGLYDMTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEY 291
            +LFRDYGLYD  MLRF    ++G   YVR DGTRSYFF  + L  LF+  G+ E+  EY
Sbjct: 225 SVLFRDYGLYDHAMLRFKASSKLGENFYVRQDGTRSYFFTDDFLAQLFMDTGYEEVVNEY 269

Query: 292 CCVKSLNRRNGKRMERVWVHGKFQKP 313
              +++N++ G  + RV++  KF KP
Sbjct: 285 VFRETVNKKEGLCVPRVFLQSKFLKP 269

BLAST of Csor.00g086280 vs. ExPASy Swiss-Prot
Match: Q8BVH9 (tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Mus musculus OX=10090 GN=Mettl6 PE=1 SV=2)

HSP 1 Score: 140.2 bits (352), Expect = 4.0e-32
Identity = 93/267 (34.83%), Postives = 130/267 (48.69%), Query Frame = 0

Query: 52  WSRFHLRHSSGKFFKERRYLLKEFPELVSCK----KNCKVLEVGCGNGSTALPILRGNEN 111
           W  F+ R+S+  FFK+R +  +EF EL SC+    +   +LE GCG G+   P+L  + N
Sbjct: 45  WDLFYKRNST-NFFKDRHWTTREFEELRSCREYEGQKLTLLEAGCGVGNCLFPLLEEDLN 104

Query: 112 IVIYACDCSTETLERAKE--IINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTS 171
           +  YACD S   ++  K+  + N        +R   F CD +                  
Sbjct: 105 LFAYACDFSPRAVDYVKQHPLYN-------AERCKVFQCDLTRDDL-------------- 164

Query: 172 LQQPSFTTPEKEGSQATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPG 231
                   PE                  VD +TLIF LSAV  ++M   +   Y  LKPG
Sbjct: 165 ---LDHVPPE-----------------SVDAVTLIFVLSAVHPEKMRLVLLNVYKVLKPG 224

Query: 232 GLLLFRDYGLYDMTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELE 291
             +LFRDYGL D  MLRF    ++G   YVR DGTRSYFF    L  LFV AG+ E+  E
Sbjct: 225 RSVLFRDYGLNDHAMLRFKAGSKLGENFYVRQDGTRSYFFTDEFLAQLFVDAGYEEVVNE 269

Query: 292 YCCVKSLNRRNGKRMERVWVHGKFQKP 313
           Y   +++N++ G  + RV++  KF+KP
Sbjct: 285 YVFRETVNKKEGLCVPRVFLQSKFRKP 269

BLAST of Csor.00g086280 vs. ExPASy Swiss-Prot
Match: Q5RDV8 (tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Pongo abelii OX=9601 GN=METTL6 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 9.9e-31
Identity = 93/266 (34.96%), Postives = 127/266 (47.74%), Query Frame = 0

Query: 52  WSRFHLRHSSGKFFKERRYLLKEFPELVSCK----KNCKVLEVGCGNGSTALPILRGNEN 111
           W  F+ R+S+  FFK+R +  +EF EL SC+    +   +LE G G G+   P+L  + N
Sbjct: 45  WDLFYKRNST-NFFKDRHWTTREFEELRSCREFEDQKLTMLEAGRGVGNCLFPLLEEDPN 104

Query: 112 IVIYACDCSTETLERAKE-IINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSL 171
           I  YACD S   +E  K+  + D       +R   F CD                  T  
Sbjct: 105 IFAYACDFSPRAVEYVKQNPLYDT------ERCKVFQCDL-----------------TKD 164

Query: 172 QQPSFTTPEKEGSQATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGG 231
                  PE                  VD + LIF LSAV   +M   ++  Y  LKPG 
Sbjct: 165 DLLDHVPPE-----------------SVDVVMLIFVLSAVHPDKMHLVLQNIYKVLKPGK 224

Query: 232 LLLFRDYGLYDMTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEY 291
            +LFRDYGLYD  MLRF    ++G   YVR DGTRSYFF    L  LF+  G+ E+  EY
Sbjct: 225 SVLFRDYGLYDHAMLRFKAGSKLGENFYVRQDGTRSYFFTDEFLAQLFMDTGYEEVVNEY 269

Query: 292 CCVKSLNRRNGKRMERVWVHGKFQKP 313
              +++N++ G  + RV++  KF KP
Sbjct: 285 VFRETVNKKEGLCVPRVFLQSKFLKP 269

BLAST of Csor.00g086280 vs. ExPASy Swiss-Prot
Match: Q8T199 (O-methyltransferase 3 OS=Dictyostelium discoideum OX=44689 GN=omt3 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.4e-29
Identity = 82/247 (33.20%), Postives = 120/247 (48.58%), Query Frame = 0

Query: 72  LKEFPELVSCK-KNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTE--TLERAKEIIN 131
           +K+  EL + + K   VLE+GCG G+T  P+L+ N     Y  D S     L ++  + N
Sbjct: 185 VKDIQELTNDESKKLTVLEIGCGTGATVYPLLKLNPEKYFYVFDFSPHAVNLVKSNSLYN 244

Query: 132 DVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQATESEESE 191
           +  + +       F CD +T + PT +                    K+ S         
Sbjct: 245 EAKLNA-------FVCDIATEQIPTSIV-------------------KDNS--------- 304

Query: 192 CCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTMLRF--GQD 251
                +D + +IF LSA+   +M A     + +LKPGG+L  RDYGLYDMT LRF   + 
Sbjct: 305 -----IDMMLMIFVLSAISRDKMHAVANSLFKSLKPGGVLYIRDYGLYDMTQLRFISKKG 364

Query: 252 QRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRMERVWVH 311
           +++    Y+R DGTR+YFF    L ++F  AGF  L  +Y   +  NR+    M RVWV 
Sbjct: 365 KKIDENFYLRADGTRTYFFTTQVLSEIFEAAGFKTLVSKYDTRELRNRKRMISMYRVWVR 391

Query: 312 GKFQKPV 314
           GKF KP+
Sbjct: 425 GKFMKPL 391

BLAST of Csor.00g086280 vs. NCBI nr
Match: KAG6599337.1 (tRNA N(3)-methylcytidine methyltransferase METTL6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030327.1 Methyltransferase-like protein 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 659 bits (1700), Expect = 3.04e-239
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS
Sbjct: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ
Sbjct: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180

Query: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240
           ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM
Sbjct: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240

Query: 241 LRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRM 300
           LRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRM
Sbjct: 241 LRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRM 300

Query: 301 ERVWVHGKFQKPVS 314
           ERVWVHGKFQKPVS
Sbjct: 301 ERVWVHGKFQKPVS 314

BLAST of Csor.00g086280 vs. NCBI nr
Match: XP_022946675.1 (methyltransferase-like protein 6 [Cucurbita moschata])

HSP 1 Score: 652 bits (1683), Expect = 1.19e-236
Identity = 311/314 (99.04%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ
Sbjct: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180

Query: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240
           ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM
Sbjct: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240

Query: 241 LRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRM 300
           LRFGQDQRVGF+EYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGK M
Sbjct: 241 LRFGQDQRVGFKEYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKSM 300

Query: 301 ERVWVHGKFQKPVS 314
           ERVWVHGKFQKPVS
Sbjct: 301 ERVWVHGKFQKPVS 314

BLAST of Csor.00g086280 vs. NCBI nr
Match: XP_022999088.1 (methyltransferase-like protein 6 [Cucurbita maxima])

HSP 1 Score: 641 bits (1654), Expect = 3.13e-232
Identity = 306/314 (97.45%), Postives = 310/314 (98.73%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGN+NIVIYACDCST+TL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNQNIVIYACDCSTKTL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           +RAKEI+NDVGIASLKDRF PFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ
Sbjct: 121 QRAKEILNDVGIASLKDRFRPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180

Query: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240
           ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM
Sbjct: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240

Query: 241 LRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRM 300
           LRFGQDQRV FREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGK M
Sbjct: 241 LRFGQDQRVEFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKSM 300

Query: 301 ERVWVHGKFQKPVS 314
           ERVWVHGKFQKPVS
Sbjct: 301 ERVWVHGKFQKPVS 314

BLAST of Csor.00g086280 vs. NCBI nr
Match: XP_023545623.1 (methyltransferase-like protein 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 638 bits (1645), Expect = 7.38e-231
Identity = 305/314 (97.13%), Postives = 308/314 (98.09%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           ERAKEI+NDVGIASLKDRF PFYCDFSTSKFPTWLACNSCRGNTSLQQ SFTTPEKEGSQ
Sbjct: 121 ERAKEILNDVGIASLKDRFRPFYCDFSTSKFPTWLACNSCRGNTSLQQTSFTTPEKEGSQ 180

Query: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240
           ATE E+SECCIGGVDFLTLIFTLSAVPLQR+PASIRECYMALKPGGLLLFRDYGLYDMTM
Sbjct: 181 ATEPEKSECCIGGVDFLTLIFTLSAVPLQRIPASIRECYMALKPGGLLLFRDYGLYDMTM 240

Query: 241 LRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRM 300
           LRFGQDQRVGFR YVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGK M
Sbjct: 241 LRFGQDQRVGFRAYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKSM 300

Query: 301 ERVWVHGKFQKPVS 314
           ERVWVHGKFQKPVS
Sbjct: 301 ERVWVHGKFQKPVS 314

BLAST of Csor.00g086280 vs. NCBI nr
Match: XP_038890924.1 (tRNA N(3)-methylcytidine methyltransferase METTL6 [Benincasa hispida])

HSP 1 Score: 595 bits (1534), Expect = 6.88e-214
Identity = 283/317 (89.27%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDF+W +LRIRVENDPLLQYHLLPF+P +S+PSVS EADSDAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFEWNDLRIRVENDPLLQYHLLPFEPSNSVPSVSPEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKK CKVLEVGCGNGST +PILRGNENIVIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIVIYACDCSTETL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           ERAKEI+N+ GIASLKDRFCPFYCDFS SKFPTWLACNSCR NT   QPSFTTPE +GS 
Sbjct: 121 ERAKEILNEAGIASLKDRFCPFYCDFSISKFPTWLACNSCRANTFQMQPSFTTPENDGSH 180

Query: 181 ATES---EESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYD 240
           AT S   EES CCIGGVDFLTLIFTLSAVPLQRMPASIREC+  LKPGGLLLFRDYGLYD
Sbjct: 181 ATGSCALEESGCCIGGVDFLTLIFTLSAVPLQRMPASIRECFTVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNG 300
           MTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFV AGFVELELEYCCV+SLNRRNG
Sbjct: 241 MTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVNAGFVELELEYCCVRSLNRRNG 300

Query: 301 KRMERVWVHGKFQKPVS 314
           K MERVWVHGKFQKPVS
Sbjct: 301 KSMERVWVHGKFQKPVS 317

BLAST of Csor.00g086280 vs. ExPASy TrEMBL
Match: A0A6J1G4K4 (Methyltransferase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450669 PE=3 SV=1)

HSP 1 Score: 652 bits (1683), Expect = 5.75e-237
Identity = 311/314 (99.04%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ
Sbjct: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180

Query: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240
           ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM
Sbjct: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240

Query: 241 LRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRM 300
           LRFGQDQRVGF+EYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGK M
Sbjct: 241 LRFGQDQRVGFKEYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKSM 300

Query: 301 ERVWVHGKFQKPVS 314
           ERVWVHGKFQKPVS
Sbjct: 301 ERVWVHGKFQKPVS 314

BLAST of Csor.00g086280 vs. ExPASy TrEMBL
Match: A0A6J1KIM0 (Methyltransferase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493570 PE=3 SV=1)

HSP 1 Score: 641 bits (1654), Expect = 1.52e-232
Identity = 306/314 (97.45%), Postives = 310/314 (98.73%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGN+NIVIYACDCST+TL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNQNIVIYACDCSTKTL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           +RAKEI+NDVGIASLKDRF PFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ
Sbjct: 121 QRAKEILNDVGIASLKDRFRPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180

Query: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240
           ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM
Sbjct: 181 ATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTM 240

Query: 241 LRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRM 300
           LRFGQDQRV FREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGK M
Sbjct: 241 LRFGQDQRVEFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKSM 300

Query: 301 ERVWVHGKFQKPVS 314
           ERVWVHGKFQKPVS
Sbjct: 301 ERVWVHGKFQKPVS 314

BLAST of Csor.00g086280 vs. ExPASy TrEMBL
Match: A0A5D3BFS2 (Methyltransferase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001150 PE=3 SV=1)

HSP 1 Score: 580 bits (1495), Expect = 2.82e-208
Identity = 277/316 (87.66%), Postives = 289/316 (91.46%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDF W++LRIRVENDP LQYHLLPF+P +SIPSVS EADS+AWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSIPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKK CKVLEVGCGNGST +PILRGNENI+IYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           ERAK+I+ND G ASLKDRF PFYCDFS SKFPTWLACNSCRGNT  QQ SFTTPE +GS 
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQQSFTTPENDGSP 180

Query: 181 ATES---EESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYD 240
           AT S   EES CCIGGVDF+TLIFTLSAVPLQRMPASIREC+M LKPGGLLLFRDYGLYD
Sbjct: 181 ATGSLALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNG 300
           MTMLRF QDQRVGFREYVRLDGTRSYFFCLNTLRDLF  AGFVELELEYCCVKS NRRNG
Sbjct: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KRMERVWVHGKFQKPV 313
           K MERVWVHGKFQKPV
Sbjct: 301 KTMERVWVHGKFQKPV 316

BLAST of Csor.00g086280 vs. ExPASy TrEMBL
Match: A0A0A0LG62 (Methyltransferase-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G009600 PE=3 SV=1)

HSP 1 Score: 580 bits (1494), Expect = 4.01e-208
Identity = 276/316 (87.34%), Postives = 290/316 (91.77%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDF W++LRIRVENDP LQYHLLPF+P +S+PSVS EADS+AWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKK CKVLEVGCGNGST +PILRGNENI+IYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           ERAK+I+ND G ASLKDRF PFYCDFS SKFPTWLACNSCRGNT  QQ SFTTPE +GSQ
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180

Query: 181 ATES---EESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYD 240
           AT S   EES CCIGGVDF+TLIFTLSAVPLQRMPASIREC+M LKPGGLLLFRDYGLYD
Sbjct: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNG 300
           MTMLRF QDQRVGFREYVRLDGTRSYFFCL+TLRDLF  AGFVELELEYCCVKS NRRNG
Sbjct: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLSTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KRMERVWVHGKFQKPV 313
           K MERVWVHGKFQKPV
Sbjct: 301 KSMERVWVHGKFQKPV 316

BLAST of Csor.00g086280 vs. ExPASy TrEMBL
Match: A0A1S3C384 (Methyltransferase-like protein OS=Cucumis melo OX=3656 GN=LOC103496346 PE=3 SV=1)

HSP 1 Score: 579 bits (1492), Expect = 8.08e-208
Identity = 277/316 (87.66%), Postives = 289/316 (91.46%), Query Frame = 0

Query: 1   MGEDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60
           MGE AEYFSKDF W++LRIRVENDP LQYHLLPF+P +SIPSVS EADS+AWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSIPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKK CKVLEVGCGNGST +PILRGNENI+IYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180
           ERAK+I+ND G ASLKDRF PFYCDFS SKFPTWLACNSCRGNT  QQ SFTTPE +GS 
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQQSFTTPENDGSP 180

Query: 181 ATES---EESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYD 240
           AT S   EES CCIGGVDF+TLIFTLSAVPLQRMPASIREC+M LKPGGLLLFRDYGLYD
Sbjct: 181 ATGSLALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNG 300
           MTMLRF QDQRVGFREYVRLDGTRSYFFCLNTLRDLF  AGFVELELEYCCVKS NRRNG
Sbjct: 241 MTMLRFVQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KRMERVWVHGKFQKPV 313
           K MERVWVHGKFQKPV
Sbjct: 301 KTMERVWVHGKFQKPV 316

BLAST of Csor.00g086280 vs. TAIR 10
Match: AT1G54650.2 (Methyltransferase family protein )

HSP 1 Score: 392.1 bits (1006), Expect = 4.2e-109
Identity = 198/312 (63.46%), Postives = 227/312 (72.76%), Query Frame = 0

Query: 3   EDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHSSG 62
           E  EY SKDF+WE L+  VENDP L +HL       S        DS  W  FH RHSSG
Sbjct: 6   EAGEYHSKDFEWEFLKNIVENDPSLSHHLHHHHQNLS----KYLPDSQPWQDFHSRHSSG 65

Query: 63  KFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETLER 122
           KFFKERRYLLKEFPELVSC +N K+LE+GCGNGST LPILRG++NI +YACDCS++ L R
Sbjct: 66  KFFKERRYLLKEFPELVSCGENSKLLEIGCGNGSTVLPILRGSKNITVYACDCSSDALVR 125

Query: 123 AKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQAT 182
            KE I D  I+S+ D F  F CDFSTS+FP W+AC+ CR    L    F           
Sbjct: 126 TKENI-DRAISSV-DNFHSFCCDFSTSEFPDWVACDRCRDKFMLNHSGF----------- 185

Query: 183 ESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTMLR 242
              ES+ CIGGVDF+TLIFTLSAVP +RMP +I+EC+  LKPGGLLLFRDYGLYDMTMLR
Sbjct: 186 GGSESKHCIGGVDFVTLIFTLSAVPKERMPRAIKECFAVLKPGGLLLFRDYGLYDMTMLR 245

Query: 243 FGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRMER 302
           F  ++RVGFREYVR DGT SYFFCL+T R LF  AGF+E+ELEYCCVK++NRR GK M R
Sbjct: 246 FEPEKRVGFREYVRSDGTLSYFFCLDTARKLFTDAGFIEVELEYCCVKAVNRRKGKDMYR 300

Query: 303 VWVHGKFQKPVS 315
           VWVHGKFQKP S
Sbjct: 306 VWVHGKFQKPFS 300

BLAST of Csor.00g086280 vs. TAIR 10
Match: AT1G54650.1 (Methyltransferase family protein )

HSP 1 Score: 389.8 bits (1000), Expect = 2.1e-108
Identity = 197/312 (63.14%), Postives = 227/312 (72.76%), Query Frame = 0

Query: 3   EDAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHSSG 62
           E  EY SKDF+WE L+  VENDP L +HL       S        DS  W  FH RHSSG
Sbjct: 6   EAGEYHSKDFEWEFLKNIVENDPSLSHHLHHHHQNLS----KYLPDSQPWQDFHSRHSSG 65

Query: 63  KFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETLER 122
           KFFKERRYLLKEFPELVSC +N K+LE+GCGNGST LPILRG++NI +YACDCS++ L R
Sbjct: 66  KFFKERRYLLKEFPELVSCGENSKLLEIGCGNGSTVLPILRGSKNITVYACDCSSDALVR 125

Query: 123 AKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQAT 182
            KE I D  I+S+ D F  F CDFSTS+FP W+AC+ CR    L               +
Sbjct: 126 TKENI-DRAISSV-DNFHSFCCDFSTSEFPDWVACDRCRDKFMLNH-------------S 185

Query: 183 ESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYDMTMLR 242
              ES+ CIGGVDF+TLIFTLSAVP +RMP +I+EC+  LKPGGLLLFRDYGLYDMTMLR
Sbjct: 186 GGSESKHCIGGVDFVTLIFTLSAVPKERMPRAIKECFAVLKPGGLLLFRDYGLYDMTMLR 245

Query: 243 FGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNGKRMER 302
           F  ++RVGFREYVR DGT SYFFCL+T R LF  AGF+E+ELEYCCVK++NRR GK M R
Sbjct: 246 FEPEKRVGFREYVRSDGTLSYFFCLDTARKLFTDAGFIEVELEYCCVKAVNRRKGKDMYR 298

Query: 303 VWVHGKFQKPVS 315
           VWVHGKFQKP S
Sbjct: 306 VWVHGKFQKPFS 298

BLAST of Csor.00g086280 vs. TAIR 10
Match: AT2G26200.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 4.1e-32
Identity = 85/267 (31.84%), Postives = 122/267 (45.69%), Query Frame = 0

Query: 46  EADSDAWSRFHLRHSSGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGN 105
           E D+  +     +H   +FFK+R YL KE+    S      +LEVGCG G+T  P++   
Sbjct: 37  ERDAKKYWDIFYKHHGDRFFKDRHYLDKEWNSYFSVSGKSVILEVGCGAGNTIFPLIATY 96

Query: 106 ENIVIYACDCSTETLERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTS 165
            +I +YACD S   +E  K           + R C F CD +       ++ +S      
Sbjct: 97  PDIFVYACDFSPRAVELVK-----AHDEYTETRVCAFACDLTGDGLDKHISPSS------ 156

Query: 166 LQQPSFTTPEKEGSQATESEESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPG 225
                                       VD +T+IF LSAV  ++M + ++     LKP 
Sbjct: 157 ----------------------------VDIVTMIFVLSAVSPEKMSSVLQNIRKVLKPN 216

Query: 226 GLLLFRDYGLYDMTMLRF-GQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVTAGFVELEL 285
           G +LFRDY + D+   RF G+DQR+    YVR DGTR+++F    L  LF   GF   EL
Sbjct: 217 GCILFRDYAVGDLAQERFSGKDQRISENFYVRGDGTRAFYFSNEFLETLFSEQGFEVEEL 264

Query: 286 EYCCVKSLNRRNGKRMERVWVHGKFQK 312
           + CC +  NR     M R WV   F++
Sbjct: 277 DVCCKQVENRSRELVMNRRWVQATFRR 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6AXU81.1e-3235.21tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Rattus norvegicus OX=10116 ... [more]
Q8TCB71.8e-3235.34tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Homo sapiens OX=9606 GN=MET... [more]
Q8BVH94.0e-3234.83tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Mus musculus OX=10090 GN=Me... [more]
Q5RDV89.9e-3134.96tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Pongo abelii OX=9601 GN=MET... [more]
Q8T1992.4e-2933.20O-methyltransferase 3 OS=Dictyostelium discoideum OX=44689 GN=omt3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6599337.13.04e-239100.00tRNA N(3)-methylcytidine methyltransferase METTL6, partial [Cucurbita argyrosper... [more]
XP_022946675.11.19e-23699.04methyltransferase-like protein 6 [Cucurbita moschata][more]
XP_022999088.13.13e-23297.45methyltransferase-like protein 6 [Cucurbita maxima][more]
XP_023545623.17.38e-23197.13methyltransferase-like protein 6 [Cucurbita pepo subsp. pepo][more]
XP_038890924.16.88e-21489.27tRNA N(3)-methylcytidine methyltransferase METTL6 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1G4K45.75e-23799.04Methyltransferase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450669 PE=... [more]
A0A6J1KIM01.52e-23297.45Methyltransferase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493570 PE=3 ... [more]
A0A5D3BFS22.82e-20887.66Methyltransferase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0LG624.01e-20887.34Methyltransferase-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G009600 PE=3 S... [more]
A0A1S3C3848.08e-20887.66Methyltransferase-like protein OS=Cucumis melo OX=3656 GN=LOC103496346 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G54650.24.2e-10963.46Methyltransferase family protein [more]
AT1G54650.12.1e-10863.14Methyltransferase family protein [more]
AT2G26200.14.1e-3231.84S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026113Methyltransferase-likePIRSFPIRSF037755Mettl2coord: 38..314
e-value: 1.6E-72
score: 242.3
IPR026113Methyltransferase-likePANTHERPTHR22809METHYLTRANSFERASE-RELATEDcoord: 3..312
NoneNo IPR availablePFAMPF13489Methyltransf_23coord: 67..280
e-value: 1.5E-11
score: 44.4
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 48..305
e-value: 2.1E-21
score: 78.1
NoneNo IPR availablePANTHERPTHR22809:SF5O-METHYLTRANSFERASE 3coord: 3..312
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 86..230
e-value: 6.54251E-5
score: 39.7207
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 50..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g086280.m01Csor.00g086280.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001510 RNA methylation
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen