Carg22126 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22126
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	beta-carotene hydroxylase 2, chloroplastic-like
Location	Carg_Chr18: 11471620 .. 11473613 (-)
RNA-Seq Expression	Carg22126
Synteny	Carg22126

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCGGCCTCTCCGCCGTCTTAGGGCCCAAACCATTCCATGTCTTCCTTACTTCCTCCAATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCATCTTCGGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAAAACGCTGCTCACCGTCTGCGTGCTCGTTGAGGATCAGAGTAGTTCCAGCGAGGTGGAAAATCTCGCAGATGAAAAATTACCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAAATTAGCGAGGAAGAAATCGGAGCGCTTCACTTATCTTGTTGCTGCAGTTATGTCTAGTTTTGGAATTACTTCCATGGCTGTCATGGCAGTTTACTACCGATTTCACTGGCAAATGGAGGTCCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCATAAAAACTTGAATTGTTTTTGTTTTCATCGCGCCGTATTCTTCCGACTAAATACTGAATTGCGACTGTTCAATTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTGCTCTCTCCGTTGGTGCCGCAGTACGTTTTTAATCTCATTTCATCTCTGTATTCTCTACTTTTCTCGAAGAATTCGATTTTCTATAGGCTGATTTTTACAATCAATTTGCGAATATCAGGTCGGCATGGAGTTTTGGGCAAGATGGGCACATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGGTATAGGTTTCTTATCCTTCTTATCTACAGAATCTCGTATTTCTGAGTTCATTTCTGCTGTGAAAATTTCTATCAACAATAATCCACGATGAACCTCAGTTTCATAGAGAACAATCAATATTCAAAACATAGAAGAATCGTTGAGGCTTGGACTTGTTAATTGCAGCACAGTCGCACTAATCACTTCTCTATAGTATTTTTTTTAATGTCTTGGCTGTTGAGAAGATTTGATTAACAATAACAGATCGTCCGCTGTGGACCTTTCTCTCATTTTCCATGTTCACTTTCCTAGCAAACAAACAACATTGAATTAAAAATGAAAAATCGTTGACGCTTGGACTTTGTTGATCTTGAATATTACAGTCTCACCACAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATAACCAACGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTTGTCCCTGGTCTCTGCTTCGGCGCAGTAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGCAATCTTTCCGAATCTGAGTCTGTTCATATCCTAACTAACCACTTGGGATTAGCGCTAATTACGGTGTTAAATGACGAAAATAGCCCTAGAAAAGTGTTGTTAATTGAGTAAGGGTTTTGACACGCTGGCGGGTTGTGGCAGGGCCTTGGAATTACTGTGTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTACTTCAGAAAGGTCGCTGCTGCTCACCAGGTAATTTAATATCATATGATATTAAATTATGTTTTTTACAGTTAAAATTAATAACATTTGTTATTAACTTGCATATAGAAAATTAATTGCACGTGTCTAAATGTAATTCTAGATTTAAAATATTATCTCCAATTTAAATTTCCAATATTATTTTCCTAATGAATTTTATGAATTTTTTGTTTCTCGACTGTAGCTTCATCATTCGGAAAAGTTCAACGGTGTTCCGTACGGCCTGTTTTTGGGTCCAAAGGTTAGTTTCGGGTCTATTTATTTTTATTTTTATGTTTATTTATTTATTTTTAATAAATGGTTTTTGCAAAAATGTTGTGGTTCAGGAATTAGAGGAAGTGGGAGGCCTAGAAGAATTGGAGAAGGAAATCAGCAGAAGAATAAAATCAACGGCCAAAAAACCAATCAACGGTTCTTGATTAACCAGTCACC

mRNA sequence

ATGGCGGCCGGCCTCTCCGCCGTCTTAGGGCCCAAACCATTCCATGTCTTCCTTACTTCCTCCAATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCATCTTCGGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAAAACGCTGCTCACCGTCTGCGTGCTCGTTGAGGATCAGAGTAGTTCCAGCGAGGTGGAAAATCTCGCAGATGAAAAATTACCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAAATTAGCGAGGAAGAAATCGGAGCGCTTCACTTATCTTGTTGCTGCAGTTATGTCTAGTTTTGGAATTACTTCCATGGCTGTCATGGCAGTTTACTACCGATTTCACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTGCTCTCTCCGTTGGTGCCGCAGTCGGCATGGAGTTTTGGGCAAGATGGGCACATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCACAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATAACCAACGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTTGTCCCTGGTCTCTGCTTCGGCGCAGGCCTTGGAATTACTGTGTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTACTTCAGAAAGGTCGCTGCTGCTCACCAGCTTCATCATTCGGAAAAGTTCAACGGTGTTCCGTACGGCCTGTTTTTGGGTCCAAAGGAATTAGAGGAAGTGGGAGGCCTAGAAGAATTGGAGAAGGAAATCAGCAGAAGAATAAAATCAACGGCCAAAAAACCAATCAACGGTTCTTGATTAACCAGTCACC

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGTCTTAGGGCCCAAACCATTCCATGTCTTCCTTACTTCCTCCAATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCATCTTCGGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAAAACGCTGCTCACCGTCTGCGTGCTCGTTGAGGATCAGAGTAGTTCCAGCGAGGTGGAAAATCTCGCAGATGAAAAATTACCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAAATTAGCGAGGAAGAAATCGGAGCGCTTCACTTATCTTGTTGCTGCAGTTATGTCTAGTTTTGGAATTACTTCCATGGCTGTCATGGCAGTTTACTACCGATTTCACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTGCTCTCTCCGTTGGTGCCGCAGTCGGCATGGAGTTTTGGGCAAGATGGGCACATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCACAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATAACCAACGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTTGTCCCTGGTCTCTGCTTCGGCGCAGGCCTTGGAATTACTGTGTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTACTTCAGAAAGGTCGCTGCTGCTCACCAGCTTCATCATTCGGAAAAGTTCAACGGTGTTCCGTACGGCCTGTTTTTGGGTCCAAAGGAATTAGAGGAAGTGGGAGGCCTAGAAGAATTGGAGAAGGAAATCAGCAGAAGAATAAAATCAACGGCCAAAAAACCAATCAACGGTTCTTGA

Protein sequence

MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVEDQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIKSTAKKPINGS

Homology

[Close]

BLAST of Carg22126 vs. NCBI nr
Match: KAG7013258.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629.0 bits (1621), Expect = 2.1e-176
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

Query: 301 STAKKPINGS 311
           STAKKPINGS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Carg22126 vs. NCBI nr
Match: KAG6574199.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 625.9 bits (1613), Expect = 1.8e-175
Identity = 309/310 (99.68%), Postives = 309/310 (99.68%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSAVLGPK FHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKSFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

Query: 301 STAKKPINGS 311
           STAKKPINGS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Carg22126 vs. NCBI nr
Match: XP_022944865.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 624.8 bits (1610), Expect = 4.0e-175
Identity = 307/310 (99.03%), Postives = 309/310 (99.68%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGL+ELEKEI+RR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

Query: 301 STAKKPINGS 311
           STAKKPINGS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Carg22126 vs. NCBI nr
Match: XP_023541483.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619.4 bits (1596), Expect = 1.7e-173
Identity = 304/310 (98.06%), Postives = 308/310 (99.35%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAA +SAVLGPKPFHVFLTSSNLSPKPRTPFL+PSSVFR+SRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAASVSAVLGPKPFHVFLTSSNLSPKPRTPFLYPSSVFRSSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQSSSSEVENLADE LPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADENLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STAKKPINGS 311
           STAKKPINGS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Carg22126 vs. NCBI nr
Match: XP_022968455.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 [Cucurbita maxima] >AEK86567.1 chloroplast beta-carotene hydroxylase [Cucurbita moschata])

HSP 1 Score: 617.1 bits (1590), Expect = 8.4e-173
Identity = 305/310 (98.39%), Postives = 306/310 (98.71%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAA LSAVLGPKPFHVFLTSSNLSPKPRTP LFPSSVFRNSRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQSSSSEVENLADEKL IIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSEK NGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STAKKPINGS 311
           STAKKPINGS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Carg22126 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 443.0 bits (1138), Expect = 2.8e-123
Identity = 222/296 (75.00%), Postives = 243/296 (82.09%), Query Frame = 0


Query: 6   SAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFR-NSRIQWKMRRKTLLTVCVLVEDQSS 65
           S  LGPK            P P TP ++P + F  N     + RR+   TVC ++ED   
Sbjct: 20  SPFLGPK------------PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKF 79

Query: 66  SSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYR 125
            ++ E   ++    I  QI +  +AEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYR
Sbjct: 80  KTQFEAGEEDIEMKIEEQISATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYR 139

Query: 126 FHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPF 185
           F+WQMEGGE+PFSEMFGTFALSVGAAVGMEFWARWAH+ALWHASLWHMHESHHKPREGPF
Sbjct: 140 FYWQMEGGEVPFSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPF 199

Query: 186 ELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPV 245
           ELNDVFAI NAVPAIALL YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPV
Sbjct: 200 ELNDVFAIINAVPAIALLDYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPV 259

Query: 246 GPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 301
           GP+ANVPY RKVAAAH LHHSEKFNGVPYGLFLGPKELEEVGGLEELEKE++RR +
Sbjct: 260 GPVANVPYLRKVAAAHSLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRRTR 303

[Close]

BLAST of Carg22126 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.3e-115
Identity = 213/302 (70.53%), Postives = 239/302 (79.14%), Query Frame = 0


Query: 18  LTSSNLSPK-----PRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE------------ 77
           ++SS+L+PK       TP L     F+      + R K  LTVC ++E            
Sbjct: 21  ISSSSLTPKSSPVSTSTPTLVVFPPFKLVSKSLRTRSKPRLTVCFVLEEKELRGKLVVAS 80

Query: 78  -DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVM 137
            D   + EV    ++++        +  +A+KLARKKSERFTYLVAAVMSSFGITSMAV+
Sbjct: 81  DDDDGAGEVRKQREKEI-----SASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVL 140

Query: 138 AVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 197
           +VYYRF WQMEGGEIP SEMFGTFALSVGAAVGMEFWARWAH ALWHASLWHMHESHHKP
Sbjct: 141 SVYYRFSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKP 200

Query: 198 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 257
           REGPFELND+FAI NAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 201 REGPFELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVH 260

Query: 258 KRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRI 302
           KRFPVGPIA+VPYFR+VAAAH LHHS+KFNGVPYGLFLGPKELEEVGGL+ LE EI+RR 
Sbjct: 261 KRFPVGPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRRT 317

[Close]

BLAST of Carg22126 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 6.4e-115
Identity = 222/316 (70.25%), Postives = 249/316 (78.80%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAAGLS  +  KP H   +SS+   + R     P S+   S     +R K   +VC +VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSL---SGFSPSLRFKR-FSVCYVVE 60

Query: 61  DQSSSSEVENLADEK------LPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGIT 120
           ++  +S +EN  DE+         I  +  +  +AEKL RKKSER TYL+AA++SSFGIT
Sbjct: 61  ERRQNSPIEN--DERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGIT 120

Query: 121 SMAVMAVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHE 180
           SMAVMAVYYRF WQMEGGEI   EMFGTFALSVGAAVGMEFWARWAHRALWHASLW+MHE
Sbjct: 121 SMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHE 180

Query: 181 SHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVH 240
           SHHKPREGPFELNDVFAI NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVH
Sbjct: 181 SHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVH 240

Query: 241 DGLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKE 300
           DGLVHKRFPVGPIA+VPY RKVAAAHQLHH++KFNGVPYGLFLGPKELEEVGG EEL+KE
Sbjct: 241 DGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDKE 300

Query: 301 ISRRIKSTAKKPINGS 311
           ISRRIKS  K   +GS
Sbjct: 301 ISRRIKSYKKASGSGS 306

[Close]

BLAST of Carg22126 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 409.5 bits (1051), Expect = 3.5e-113
Identity = 204/283 (72.08%), Postives = 234/283 (82.69%), Query Frame = 0


Query: 30  PFLFPSSVFRNSRIQWKMRRKTLLTVCVLVED-------QSSSSEVENLADE-KLPIIVP 89
           P LF S +  N     + RRK  L  C +++D           S+ E + DE ++     
Sbjct: 32  PLLFFSPLTCNLDAILRSRRKPRLAACFVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEE 91

Query: 90  QIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFHWQMEGGEIPFSEMFG 149
           +  +  +AEK ARKKSERFTYLVAAVMSS GITSMAV++VYYRF WQMEGGE+PFSEMF 
Sbjct: 92  KSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFC 151

Query: 150 TFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAITNAVPAIAL 209
           TFAL+ GAA+GME+WARWAHRALWHASLWHMHESHH+PREGPFELND+FAI NAVPAIAL
Sbjct: 152 TFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINAVPAIAL 211

Query: 210 LSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQ 269
           LS+GF HKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYF++VAAAHQ
Sbjct: 212 LSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRVAAAHQ 271

Query: 270 LHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIKSTAK 305
           LHHS+KF+GVPYGLFLGPKELEEVG LEELEKE++RRIKS+ +
Sbjct: 272 LHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIKSSKR 314

[Close]

BLAST of Carg22126 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 3.0e-112
Identity = 212/305 (69.51%), Postives = 242/305 (79.34%), Query Frame = 0


Query: 1   MAAGLSAV-LGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLV 60
           MAAGLS + +  KP +    S+N    P +  +FP S+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQSSSSEVENLADEKLPIIVPQ----IPSPHVAEKLARKKSERFTYLVAAVMSSFGITS 120
           E++  SS ++   D+  P         + +  + +K  +KKSERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTFALSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
           HHKPREG FELNDVFAITNAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHD
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHD 240

Query: 241 GLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEI 300
           GLVHKRFPVGPIANVPY RKVAAAHQLHH++KF GVPYGLFLGPKE+EEVGG EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 294

[Close]

BLAST of Carg22126 vs. ExPASy TrEMBL
Match: A0A6J1FZ94 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449265 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.0e-175
Identity = 307/310 (99.03%), Postives = 309/310 (99.68%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGL+ELEKEI+RR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

Query: 301 STAKKPINGS 311
           STAKKPINGS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Carg22126 vs. ExPASy TrEMBL
Match: A0A6J1HX82 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467695 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.1e-173
Identity = 305/310 (98.39%), Postives = 306/310 (98.71%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAA LSAVLGPKPFHVFLTSSNLSPKPRTP LFPSSVFRNSRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQSSSSEVENLADEKL IIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSEK NGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STAKKPINGS 311
           STAKKPINGS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Carg22126 vs. ExPASy TrEMBL
Match: G1FQR0 (Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata OX=3662 GN=CHYB PE=2 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.1e-173
Identity = 305/310 (98.39%), Postives = 306/310 (98.71%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAA LSAVLGPKPFHVFLTSSNLSPKPRTP LFPSSVFRNSRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQSSSSEVENLADEKL IIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSEK NGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STAKKPINGS 311
           STAKKPINGS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Carg22126 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.4e-160
Identity = 278/310 (89.68%), Postives = 298/310 (96.13%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSA   PKPFH+FLTSS+LSPKP +PFLFP SVFRNSR QWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
            Q+SS +V+NL+DE+ PI+VPQIPSPHV+E+LARKKSERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STAKKPINGS 311
            TA+K I+GS
Sbjct: 301 WTARKSIDGS 310

[Close]

BLAST of Carg22126 vs. ExPASy TrEMBL
Match: A0A6J1J1S8 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482629 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.7e-159
Identity = 278/310 (89.68%), Postives = 293/310 (94.52%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAA LSA LGPKP H+FL SS+LS KPRTPFLFP SVFRNSRIQ KMRRKTL TVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENL+DE+ PI+VPQIPSP+VA++LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF LSVGAAVGMEFWARWAHRALWH+S+WHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKPR 180

Query: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG EELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 STAKKPINGS 311
            T  K INGS
Sbjct: 301 LTTTKSINGS 310

[Close]

BLAST of Carg22126 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 415.2 bits (1066), Expect = 4.5e-116
Identity = 222/316 (70.25%), Postives = 249/316 (78.80%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAAGLS  +  KP H   +SS+   + R     P S+   S     +R K   +VC +VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSL---SGFSPSLRFKR-FSVCYVVE 60

Query: 61  DQSSSSEVENLADEK------LPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGIT 120
           ++  +S +EN  DE+         I  +  +  +AEKL RKKSER TYL+AA++SSFGIT
Sbjct: 61  ERRQNSPIEN--DERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGIT 120

Query: 121 SMAVMAVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHE 180
           SMAVMAVYYRF WQMEGGEI   EMFGTFALSVGAAVGMEFWARWAHRALWHASLW+MHE
Sbjct: 121 SMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHE 180

Query: 181 SHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVH 240
           SHHKPREGPFELNDVFAI NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVH
Sbjct: 181 SHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVH 240

Query: 241 DGLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKE 300
           DGLVHKRFPVGPIA+VPY RKVAAAHQLHH++KFNGVPYGLFLGPKELEEVGG EEL+KE
Sbjct: 241 DGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDKE 300

Query: 301 ISRRIKSTAKKPINGS 311
           ISRRIKS  K   +GS
Sbjct: 301 ISRRIKSYKKASGSGS 306

[Close]

BLAST of Carg22126 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 406.4 bits (1043), Expect = 2.1e-113
Identity = 212/305 (69.51%), Postives = 242/305 (79.34%), Query Frame = 0


Query: 1   MAAGLSAV-LGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLV 60
           MAAGLS + +  KP +    S+N    P +  +FP S+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQSSSSEVENLADEKLPIIVPQ----IPSPHVAEKLARKKSERFTYLVAAVMSSFGITS 120
           E++  SS ++   D+  P         + +  + +K  +KKSERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTFALSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
           HHKPREG FELNDVFAITNAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHD
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHD 240

Query: 241 GLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEI 300
           GLVHKRFPVGPIANVPY RKVAAAHQLHH++KF GVPYGLFLGPKE+EEVGG EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 294

[Close]

BLAST of Carg22126 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 257.3 bits (656), Expect = 1.6e-68
Identity = 144/225 (64.00%), Postives = 165/225 (73.33%), Query Frame = 0


Query: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60
           MAAGLS  +  KP H   +SS+   + R     P S+   S     +R K   +VC +VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSL---SGFSPSLRFKR-FSVCYVVE 60

Query: 61  DQSSSSEVENLADEK------LPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGIT 120
           ++  +S +EN  DE+         I  +  +  +AEKL RKKSER TYL+AA++SSFGIT
Sbjct: 61  ERRQNSPIEN--DERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGIT 120

Query: 121 SMAVMAVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHE 180
           SMAVMAVYYRF WQMEGGEI   EMFGTFALSVGAAVGMEFWARWAHRALWHASLW+MHE
Sbjct: 121 SMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHE 180

Query: 181 SHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGA 220
           SHHKPREGPFELNDVFAI NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 SHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]

BLAST of Carg22126 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 243.0 bits (619), Expect = 3.1e-64
Identity = 150/305 (49.18%), Postives = 178/305 (58.36%), Query Frame = 0


Query: 1   MAAGLSAV-LGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLV 60
           MAAGLS + +  KP +    S+N    P +  +FP S+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQSSSSEVENLADEKLPIIVPQ----IPSPHVAEKLARKKSERFTYLVAAVMSSFGITS 120
           E++  SS ++   D+  P         + +  + +K  +KKSERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTFALSVGA                          
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGA-------------------------- 180

Query: 181 HHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
                                                      AGLGIT+FGMAYMFVHD
Sbjct: 181 -------------------------------------------AGLGITMFGMAYMFVHD 225

Query: 241 GLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEI 300
           GLVHKRFPVGPIANVPY RKVAAAHQLHH++KF GVPYGLFLGPKE+EEVGG EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 225

The following BLAST results are available for this feature:

BLAST of Carg22126 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013258.1	2.1e-176	100.00	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
KAG6574199.1	1.8e-175	99.68	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_022944865.1	4.0e-175	99.03	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
XP_023541483.1	1.7e-173	98.06	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp...	[more]
XP_022968455.1	8.4e-173	98.39	beta-carotene hydroxylase 2, chloroplastic-like isoform X2 [Cucurbita maxima] >A...	[more]

BLAST of Carg22126 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	2.8e-123	75.00	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	1.3e-115	70.53	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9SZZ8	6.4e-115	70.25	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49815	3.5e-113	72.08	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]
Q9LTG0	3.0e-112	69.51	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Carg22126 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ94	2.0e-175	99.03	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata...	[more]
A0A6J1HX82	4.1e-173	98.39	beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima O...	[more]
G1FQR0	4.1e-173	98.39	Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata OX=3662 GN=CHYB PE=2...	[more]
A0A0A0L606	1.4e-160	89.68	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1J1S8	9.7e-159	89.68	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Carg22126 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	4.5e-116	70.25	beta-hydroxylase 1	[more]
AT5G52570.1	2.1e-113	69.51	beta-carotene hydroxylase 2	[more]
AT4G25700.2	1.6e-68	64.00	beta-hydroxylase 1	[more]
AT5G52570.2	3.1e-64	49.18	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 149..276 e-value: 1.8E-13 score: 50.9
None	No IPR available	PANTHER	PTHR31899:SF10	BETA-CAROTENE HYDROXYLASE	coord: 16..301
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 16..301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22126-RA	Carg22126-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity