Carg06630 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06630
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Auxin efflux carrier component
Location	Carg_Chr18: 331080 .. 332304 (-)
RNA-Seq Expression	Carg06630
Synteny	Carg06630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCTTTTTGGCATGGTTTAAGAACGTCCATAGCAAAGACAGTATATTCTAGCGGTAGGCTCGAGCTAACCATTATAATTCAAAATCACCCTTTGCATGTATCTTAGAGTTGTAGGAAGGATCGTACGAATTATCTCGGAAAAATAGTTAAGATATATGTGAATTGGCTCGAAAATAACCTAGCAATATTGGACAAGTTATCTCGTGAGAATAGTTAAGGTGTTTGTATGGCTTGAAATGGGTACTCACCCATACAAAAGACCTAGTTGTAAGGTCGGACAAGTCATCTCGGGAGAGAATAATTAAGGTGTCTATGAACTGAGTCATGTAAGTTATCCCGTGAAAATAGTCAAGGTATCTCTCAGCTCTGGCTTGGTTGGTAGAGTCATATAAGTTATCCGGTGAAAATAGTTAAGGTATCCCCTCAACTTGGACCTGGTTGGTAGAGAGTCATGACAGTTACCCCGTGAAAATAGTTAAGATATCTCTCTAACTCGGGCTTAGTTGGTAGAGTCATGTAGTTAAGGAGACATCTCTCAGCTCGAGCCTTGTAGATAGAGTCAGGTAAGTTATCCCGTGAAAATAGATAAGGTATCTCTTAGCTCGGGCCTAGCTTGGTTGTTGATTGTTGATGTGTTTGGAAACAAACATCTGTAGCTAGGTTTGTTTAACATGTTTTTGTGTCCCATTAGTTTGGAGTTTCCTACAAATTTATATCCATTTTTTGTTTATAAAAAAACAGGCAGCTCTCCCACAAGGAATTGTCCCCTTTGTCTTTGCGAAGGAGTACAACGTACACCCTGATATTCTCAGCACAGGGTATGTATAGCACCAATATCCTTAATAACTCCGACATTAAATCTCTGTTTTGTTTCAAAAAAACACAAAAAGATTTGATTTTTCTTCGTGGGTTTGCAGAGTTATCTTTGGAATGTTAATCGCTTTGCCTATCACGCTTGTTTACTACATTTTGCTGGGGATTTGAGGCTGCCATGGAAGCCAAGGTCAGCCATGGCTGCGTCCGCCATGGGAGACCATTTCAAAAGCTTCAAAGCTTTGGGAGAGAAGATTTTAACAGGGAAAAAGATAAAGGAGAAGAAGAAAGAGGAGAGATTTGAGGGAAATGGGGAAAATTCTTCCAAGAAAAGGGGAAAAAACAGGCTGTAAAATCATTATGTTTGGTAGTCAATTATATAAGAAAAAAAATGTTTTGGTTTTTTT

mRNA sequence

ATGGTTGCTTTTTGGCATGGTTTAAGAACGTCCATAGCAAAGACAGCAGCTCTCCCACAAGGAATTGTCCCCTTTGTCTTTGCGAAGGAGTACAACAGTTATCTTTGGAATGTTAATCGCTTTGCCTATCACGCTTGTTTACTACATTTTGCTGGGGATTTGAGGCTGCCATGGAAGCCAAGGTCAGCCATGGCTGCGTCCGCCATGGGAGACCATTTCAAAAGCTTCAAAGCTTTGGGAGAGAAGATTTTAACAGGGAAAAAGATAAAGGAGAAGAAGAAAGAGGAGAGATTTGAGGGAAATGGGGAAAATTCTTCCAAGAAAAGGGGAAAAAACAGGCTGTAAAATCATTATGTTTGGTAGTCAATTATATAAGAAAAAAAATGTTTTGGTTTTTTT

Coding sequence (CDS)

ATGGTTGCTTTTTGGCATGGTTTAAGAACGTCCATAGCAAAGACAGCAGCTCTCCCACAAGGAATTGTCCCCTTTGTCTTTGCGAAGGAGTACAACAGTTATCTTTGGAATGTTAATCGCTTTGCCTATCACGCTTGTTTACTACATTTTGCTGGGGATTTGAGGCTGCCATGGAAGCCAAGGTCAGCCATGGCTGCGTCCGCCATGGGAGACCATTTCAAAAGCTTCAAAGCTTTGGGAGAGAAGATTTTAACAGGGAAAAAGATAAAGGAGAAGAAGAAAGAGGAGAGATTTGAGGGAAATGGGGAAAATTCTTCCAAGAAAAGGGGAAAAAACAGGCTGTAA

Protein sequence

MVAFWHGLRTSIAKTAALPQGIVPFVFAKEYNSYLWNVNRFAYHACLLHFAGDLRLPWKPRSAMAASAMGDHFKSFKALGEKILTGKKIKEKKKEERFEGNGENSSKKRGKNRL

Homology

[Close]

BLAST of Carg06630 vs. NCBI nr
Match: KAG7012151.1 (hypothetical protein SDJN02_24903, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 230.7 bits (587), Expect = 6.2e-57
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MVAFWHGLRTSIAKTAALPQGIVPFVFAKEYNSYLWNVNRFAYHACLLHFAGDLRLPWKP 60
           MVAFWHGLRTSIAKTAALPQGIVPFVFAKEYNSYLWNVNRFAYHACLLHFAGDLRLPWKP
Sbjct: 1   MVAFWHGLRTSIAKTAALPQGIVPFVFAKEYNSYLWNVNRFAYHACLLHFAGDLRLPWKP 60

Query: 61  RSAMAASAMGDHFKSFKALGEKILTGKKIKEKKKEERFEGNGENSSKKRGKNRL 115
           RSAMAASAMGDHFKSFKALGEKILTGKKIKEKKKEERFEGNGENSSKKRGKNRL
Sbjct: 61  RSAMAASAMGDHFKSFKALGEKILTGKKIKEKKKEERFEGNGENSSKKRGKNRL 114

[Close]

BLAST of Carg06630 vs. NCBI nr
Match: RXH77229.1 (hypothetical protein DVH24_023503 [Malus domestica])

HSP 1 Score: 72.0 bits (175), Expect = 3.7e-09
Identity = 30/40 (75.00%), Postives = 35/40 (87.50%), Query Frame = 0


Query: 16  AALPQGIVPFVFAKEYNSYLWNVNRFAYHACLLHFAGDLR 56
           AALPQGIVPFVFAKEYN Y+WNV+  A+HACLLHF G ++
Sbjct: 576 AALPQGIVPFVFAKEYNGYIWNVDCIAHHACLLHFVGAMK 615

[Close]

BLAST of Carg06630 vs. NCBI nr
Match: RVW21396.1 (Auxin efflux carrier component 1a [Vitis vinifera])

HSP 1 Score: 61.6 bits (148), Expect = 5.0e-06
Identity = 30/56 (53.57%), Postives = 40/56 (71.43%), Query Frame = 0


Query: 8   LRTSIAKTAALPQGIVPFVFAKEYN--------SYLWNVNRFAYHACLLHFAGDLR 56
           LR +I + AALPQGIVPFVFAKEYN         ++W+VN  A++ CLLHF G+++
Sbjct: 553 LRVAIVQ-AALPQGIVPFVFAKEYNVHPDILSTGHIWDVNCIAHYTCLLHFTGNMK 607

[Close]

BLAST of Carg06630 vs. NCBI nr
Match: KAG6430972.1 (hypothetical protein SASPL_109046 [Salvia splendens])

HSP 1 Score: 59.3 bits (142), Expect = 2.5e-05
Identity = 40/94 (42.55%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 16  AALPQGIVPFVFAKEYNSY----------LWNVNRFAYHACLLHFAGDLRLPWKPRSAMA 75
           A+LPQGIVPFVFAKEYN +          +W+VN  ++H+CLLHFAG L++     +A  
Sbjct: 511 ASLPQGIVPFVFAKEYNVHPDILSTGLCDIWDVNSSSHHSCLLHFAGALKMNLHTTNAF- 570

Query: 76  ASAMGDHFKSFKALGEKILTG---KKIKEKKKEE 97
             A     K  +    KIL     KK  +KKK+E
Sbjct: 571 FWASNRREKKLEEGYWKILQAPNIKKFSQKKKKE 603

[Close]

BLAST of Carg06630 vs. NCBI nr
Match: XP_024628438.1 (probable auxin efflux carrier component 1c isoform X2 [Medicago truncatula])

HSP 1 Score: 55.8 bits (133), Expect = 2.7e-04
Identity = 25/39 (64.10%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 16  AALPQGIVPFVFAKEYNSYLWNVNRFAYHACLLHFAGDL 55
           AALPQGIVPFVFAKEYN   W+V+  A++A LLH  G +
Sbjct: 478 AALPQGIVPFVFAKEYNGNFWDVDCIAHNARLLHLVGSM 516

[Close]

BLAST of Carg06630 vs. ExPASy TrEMBL
Match: A0A498I2V7 (Auxin efflux carrier component OS=Malus domestica OX=3750 GN=DVH24_023503 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.8e-09
Identity = 30/40 (75.00%), Postives = 35/40 (87.50%), Query Frame = 0


Query: 16  AALPQGIVPFVFAKEYNSYLWNVNRFAYHACLLHFAGDLR 56
           AALPQGIVPFVFAKEYN Y+WNV+  A+HACLLHF G ++
Sbjct: 576 AALPQGIVPFVFAKEYNGYIWNVDCIAHHACLLHFVGAMK 615

[Close]

BLAST of Carg06630 vs. ExPASy TrEMBL
Match: A0A438CDZ1 (Auxin efflux carrier component OS=Vitis vinifera OX=29760 GN=PIN1A_1 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.4e-06
Identity = 30/56 (53.57%), Postives = 40/56 (71.43%), Query Frame = 0


Query: 8   LRTSIAKTAALPQGIVPFVFAKEYN--------SYLWNVNRFAYHACLLHFAGDLR 56
           LR +I + AALPQGIVPFVFAKEYN         ++W+VN  A++ CLLHF G+++
Sbjct: 553 LRVAIVQ-AALPQGIVPFVFAKEYNVHPDILSTGHIWDVNCIAHYTCLLHFTGNMK 607

[Close]

BLAST of Carg06630 vs. ExPASy TrEMBL
Match: A0A4D9BL89 (Auxin efflux carrier component OS=Salvia splendens OX=180675 GN=PIN PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.2e-05
Identity = 40/94 (42.55%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 16  AALPQGIVPFVFAKEYNSY----------LWNVNRFAYHACLLHFAGDLRLPWKPRSAMA 75
           A+LPQGIVPFVFAKEYN +          +W+VN  ++H+CLLHFAG L++     +A  
Sbjct: 511 ASLPQGIVPFVFAKEYNVHPDILSTGLCDIWDVNSSSHHSCLLHFAGALKMNLHTTNAF- 570

Query: 76  ASAMGDHFKSFKALGEKILTG---KKIKEKKKEE 97
             A     K  +    KIL     KK  +KKK+E
Sbjct: 571 FWASNRREKKLEEGYWKILQAPNIKKFSQKKKKE 603

[Close]

BLAST of Carg06630 vs. ExPASy TrEMBL
Match: A0A396GS05 (Auxin efflux carrier component OS=Medicago truncatula OX=3880 GN=MtrunA17_Chr8g0393111 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.3e-04
Identity = 25/39 (64.10%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 16  AALPQGIVPFVFAKEYNSYLWNVNRFAYHACLLHFAGDL 55
           AALPQGIVPFVFAKEYN   W+V+  A++A LLH  G +
Sbjct: 501 AALPQGIVPFVFAKEYNGNFWDVDCIAHNARLLHLVGSM 539

[Close]

BLAST of Carg06630 vs. ExPASy TrEMBL
Match: A0A438J7D3 (Auxin efflux carrier component OS=Vitis vinifera OX=29760 GN=PIN1C_4 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.3e-04
Identity = 28/56 (50.00%), Postives = 34/56 (60.71%), Query Frame = 0


Query: 16  AALPQGIVPFVFAKEYN---------------SYLWNVNRFAYHACLLHFAGDLRL 57
           AALPQGIVPFVFAKEYN                Y+W+ N F ++A LLH AG L++
Sbjct: 522 AALPQGIVPFVFAKEYNVHPDILSTGFEPDALGYIWDANSFTHYAGLLHSAGALKM 577

[Close]

BLAST of Carg06630 vs. TAIR 10
Match: AT2G01420.1 (Auxin efflux carrier family protein )

HSP 1 Score: 40.8 bits (94), Expect = 8.5e-04
Identity = 22/29 (75.86%), Postives = 24/29 (82.76%), Query Frame = 0


Query: 6   HG--LRTSIAKTAALPQGIVPFVFAKEYN 33
           HG  LR +I + AALPQGIVPFVFAKEYN
Sbjct: 555 HGDLLRIAIVQ-AALPQGIVPFVFAKEYN 582

[Close]

BLAST of Carg06630 vs. TAIR 10
Match: AT2G01420.2 (Auxin efflux carrier family protein )

HSP 1 Score: 40.8 bits (94), Expect = 8.5e-04
Identity = 22/29 (75.86%), Postives = 24/29 (82.76%), Query Frame = 0


Query: 6   HG--LRTSIAKTAALPQGIVPFVFAKEYN 33
           HG  LR +I + AALPQGIVPFVFAKEYN
Sbjct: 559 HGDLLRIAIVQ-AALPQGIVPFVFAKEYN 586

The following BLAST results are available for this feature:

BLAST of Carg06630 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012151.1	6.2e-57	100.00	hypothetical protein SDJN02_24903, partial [Cucurbita argyrosperma subsp. argyro...	[more]
RXH77229.1	3.7e-09	75.00	hypothetical protein DVH24_023503 [Malus domestica]	[more]
RVW21396.1	5.0e-06	53.57	Auxin efflux carrier component 1a [Vitis vinifera]	[more]
KAG6430972.1	2.5e-05	42.55	hypothetical protein SASPL_109046 [Salvia splendens]	[more]
XP_024628438.1	2.7e-04	64.10	probable auxin efflux carrier component 1c isoform X2 [Medicago truncatula]	[more]

BLAST of Carg06630 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg06630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A498I2V7	1.8e-09	75.00	Auxin efflux carrier component OS=Malus domestica OX=3750 GN=DVH24_023503 PE=3 S...	[more]
A0A438CDZ1	2.4e-06	53.57	Auxin efflux carrier component OS=Vitis vinifera OX=29760 GN=PIN1A_1 PE=3 SV=1	[more]
A0A4D9BL89	1.2e-05	42.55	Auxin efflux carrier component OS=Salvia splendens OX=180675 GN=PIN PE=3 SV=1	[more]
A0A396GS05	1.3e-04	64.10	Auxin efflux carrier component OS=Medicago truncatula OX=3880 GN=MtrunA17_Chr8g0...	[more]
A0A438J7D3	2.3e-04	50.00	Auxin efflux carrier component OS=Vitis vinifera OX=29760 GN=PIN1C_4 PE=3 SV=1	[more]

BLAST of Carg06630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G01420.1	8.5e-04	75.86	Auxin efflux carrier family protein	[more]
AT2G01420.2	8.5e-04	75.86	Auxin efflux carrier family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 90..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06630-RA	Carg06630-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009734	auxin-activated signaling pathway
biological_process	GO:0060918	auxin transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane