Carg12144 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12144
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionacyl-protein thioesterase 2-like
LocationCarg_Chr11: 2452090 .. 2453535 (+)
RNA-Seq ExpressionCarg12144
SyntenyCarg12144
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAAAACTGCCAGAAAGGCAATTGAGTTTGGTAAAACCCATGTAGTGAGGCCCAAGGGAAGACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCGCAAGGTTTTCTTCTTTCTTTCTTTCTTTCTGTTCTTTTCTTAATTGTTTTTATTATGTTTAGCAATGTTTTGATTTTGTTCGACAGCTGGTCGCAGCTCTTGGAGACCCTTCCCCTACCAAATGTAAGAATATGAAGCTTATCAAACTATTTATAAGCATTTACACTGCTATTCTCCATGGTGGGAAGATGAATTTAAGCTTGGAGTTATTGATGCAGATTAAATGGATATGTCCAACTGCACCTACTCGACCGATAGCTTTATTTGGTGGCTTCCCTTCCACTGCTTGTAAGCTTTACTGAAATCCCTTTTCCTTTAATTATTCAACGTCGCTCGAACCGGGATCTCCATGCTGCAAAGTCTAGTTGTTATTCATGTATTTTACAATAACAAGAGCATCATGACTGATATCTTATATATTCAAAGACTGATGTTCTTGTTAAACCAGGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTAGAGGGGTTGGATGCTTCAGCTGCTCACGTTGCATATTTGTTATCAACAGAGCCATCTGACAGTAAGTTTCTCTTGCCCTTCTAAATACTATTTGTTTTTGCTTTTTCTACTGATTGTTGGCGTTATTCTGCTGTAATCTTTTTTACAGTTAAGCTTGCTGTAGGAGGGTTCAGTATGGGAGCAGCTACTGCGCTTTACTCGGCGACCTGCCATTCACTGGGAAAATATGGGAATGGCAATCCTTATCCAGCCAATTTGAGTGCAGTTGTGGGGCTTAGTGGCTGGCTTCCATGTTCAAAGTTGGCTTTTTAATTTTGTCATCTGTACAATCTTTGATCCTTTTAAAATCAATAGAAAGCTTCATTTAACGAGTTATCGTGGTGATTCCAGGACTTTGAAGAGCAAGATGGAAGAGATGAAAGCTAGTAATAGTGGAGCGGGATCATTGCCTGTTTTGCTATGTCATGGAAAAGGTATTTATGAATTTATAATTGTTCATGCAGAATATTACGTTACAAATTTGAGATTGTTGCATTTCTGATAATGGTTTGGTGTGAAACGTGTTGCAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCACAGGCATTACGTTCATGTGGATTTCAAGACGTTACGTTTAAATCCTACAACACGTATGTGACTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAATCAAGAAACTAAGTAACGTGTTGTTTGATATCGAGTCGTGTTTTGTTTACAGACTTGGGCATTACACAGTTCCTGAAGAGATGGATGAGGTTTGCGCCTGGTTAACTTCAAAGTTGGGGCTATAG

mRNA sequence

GTTAAAACTGCCAGAAAGGCAATTGAGTTTGGTAAAACCCATGTAGTGAGGCCCAAGGGAAGACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCGCAAGCTGGTCGCAGCTCTTGGAGACCCTTCCCCTACCAAATATTAAATGGATATGTCCAACTGCACCTACTCGACCGATAGCTTTATTTGGTGGCTTCCCTTCCACTGCTTGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTAGAGGGGTTGGATGCTTCAGCTGCTCACGTTGCATATTTGTTATCAACAGAGCCATCTGACATTAAGCTTGCTGTAGGAGGGTTCAGTATGGGAGCAGCTACTGCGCTTTACTCGGCGACCTGCCATTCACTGGGAAAATATGGGAATGGCAATCCTTATCCAGCCAATTTGAGTGCAGTTGTGGGGCTTAGTGGCTGGCTTCCATGTTCAAAGACTTTGAAGAGCAAGATGGAAGAGATGAAAGCTAGTAATAGTGGAGCGGGATCATTGCCTGTTTTGCTATGTCATGGAAAAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCACAGGCATTACGTTCATGTGGATTTCAAGACGTTACGTTTAAATCCTACAACACACTTGGGCATTACACAGTTCCTGAAGAGATGGATGAGGTTTGCGCCTGGTTAACTTCAAAGTTGGGGCTATAG

Coding sequence (CDS)

GTTAAAACTGCCAGAAAGGCAATTGAGTTTGGTAAAACCCATGTAGTGAGGCCCAAGGGAAGACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCGCAAGCTGGTCGCAGCTCTTGGAGACCCTTCCCCTACCAAATATTAAATGGATATGTCCAACTGCACCTACTCGACCGATAGCTTTATTTGGTGGCTTCCCTTCCACTGCTTGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTAGAGGGGTTGGATGCTTCAGCTGCTCACGTTGCATATTTGTTATCAACAGAGCCATCTGACATTAAGCTTGCTGTAGGAGGGTTCAGTATGGGAGCAGCTACTGCGCTTTACTCGGCGACCTGCCATTCACTGGGAAAATATGGGAATGGCAATCCTTATCCAGCCAATTTGAGTGCAGTTGTGGGGCTTAGTGGCTGGCTTCCATGTTCAAAGACTTTGAAGAGCAAGATGGAAGAGATGAAAGCTAGTAATAGTGGAGCGGGATCATTGCCTGTTTTGCTATGTCATGGAAAAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCACAGGCATTACGTTCATGTGGATTTCAAGACGTTACGTTTAAATCCTACAACACACTTGGGCATTACACAGTTCCTGAAGAGATGGATGAGGTTTGCGCCTGGTTAACTTCAAAGTTGGGGCTATAG

Protein sequence

VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL
Homology
BLAST of Carg12144 vs. NCBI nr
Match: KAG7021798.1 (Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240

Query: 241 L 242
           L
Sbjct: 241 L 241

BLAST of Carg12144 vs. NCBI nr
Match: XP_022925495.1 (acyl-protein thioesterase 2-like [Cucurbita moschata] >XP_022925502.1 acyl-protein thioesterase 2-like [Cucurbita moschata] >XP_023530534.1 acyl-protein thioesterase 2-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023530535.1 acyl-protein thioesterase 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 12  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 71

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 72  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 131

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 132 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 191

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 192 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 251

Query: 241 L 242
           L
Sbjct: 252 L 252

BLAST of Carg12144 vs. NCBI nr
Match: KAG6587912.1 (Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 118 VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 177

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 178 RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 237

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 238 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 297

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 298 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 357

Query: 241 L 242
           L
Sbjct: 358 L 358

BLAST of Carg12144 vs. NCBI nr
Match: XP_023530533.1 (acyl-protein thioesterase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 36  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 95

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 96  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 155

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 156 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 215

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 216 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 275

Query: 241 L 242
           L
Sbjct: 276 L 276

BLAST of Carg12144 vs. NCBI nr
Match: XP_023004193.1 (acyl-protein thioesterase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 502.3 bits (1292), Expect = 2.3e-138
Identity = 240/241 (99.59%), Postives = 240/241 (99.59%), Query Frame = 0

Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 36  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 95

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 96  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 155

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKM EMKASNSGAGSLPV
Sbjct: 156 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMVEMKASNSGAGSLPV 215

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 216 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 275

Query: 241 L 242
           L
Sbjct: 276 L 276

BLAST of Carg12144 vs. ExPASy Swiss-Prot
Match: O95372 (Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.2e-32
Identity = 82/225 (36.44%), Postives = 123/225 (54.67%), Query Frame = 0

Query: 21  RHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 80
           R  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 81  SEDGPDDLEGLDASAAHVAYLLSTEPSD----IKLAVGGFSMGAATALYSA-TCHSLGKY 140
           S D P+D  G+  +A ++  L+  E  +     ++ +GGFS G A +LY+A TC      
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTC------ 141

Query: 141 GNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKF 200
               P+P  L+ +V LS WLP  +         +A+N  A  L +L CHG++D +V  +F
Sbjct: 142 ----PHP--LAGIVALSCWLPLHRAFP------QAANGSAKDLAILQCHGELDPMVPVRF 201

Query: 201 GEKSSQALRSCGF-QDVTFKSYNTLGHYTVPEEMDEVCAWLTSKL 240
           G  +++ LRS      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 GALTAEKLRSVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Carg12144 vs. ExPASy Swiss-Prot
Match: Q9WTL7 (Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-31
Identity = 81/225 (36.00%), Postives = 123/225 (54.67%), Query Frame = 0

Query: 21  RHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 80
           R  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 81  SEDGPDDLEGLDASAAHVAYLLSTEPSD----IKLAVGGFSMGAATALYSA-TCHSLGKY 140
           S D P+D  G+  +A ++  L+  E  +     ++ +GGFS G A +LY+A TC      
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTC------ 141

Query: 141 GNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKF 200
               P+P  L+ +V LS WLP  +         +A+N  A  L +L CHG++D +V  +F
Sbjct: 142 ----PHP--LAGIVALSCWLPLHRNFP------QAANGSAKDLAILQCHGELDPMVPVRF 201

Query: 201 GEKSSQALRSCGF-QDVTFKSYNTLGHYTVPEEMDEVCAWLTSKL 240
           G  +++ LR+      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 GALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Carg12144 vs. ExPASy Swiss-Prot
Match: Q9QYL8 (Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-31
Identity = 81/225 (36.00%), Postives = 123/225 (54.67%), Query Frame = 0

Query: 21  RHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 80
           R  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 81  SEDGPDDLEGLDASAAHVAYLLSTEPSD----IKLAVGGFSMGAATALYSA-TCHSLGKY 140
           S D P+D  G+  +A ++  L+  E  +     ++ +GGFS G A +LY+A TC      
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTC------ 141

Query: 141 GNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKF 200
               P+P  L+ +V LS WLP  +         +A+N  A  L +L CHG++D +V  +F
Sbjct: 142 ----PHP--LAGIVALSCWLPLHRNFP------QAANGSAKDLAILQCHGELDPMVPVRF 201

Query: 201 GEKSSQALRSCGF-QDVTFKSYNTLGHYTVPEEMDEVCAWLTSKL 240
           G  +++ LR+      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 GALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Carg12144 vs. ExPASy Swiss-Prot
Match: Q4I8Q4 (Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=FGRAMPH1_01T20223 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.3e-27
Identity = 79/233 (33.91%), Postives = 122/233 (52.36%), Query Frame = 0

Query: 21  RHQATVVWLHGLGDNGASWSQLLET----LPLPNIKWICPTAPTRPIALFGGFPSTAWFD 80
           +H ATV+++HGLGD G  W+  +E       +  +K+I P AP  PI++  G     WFD
Sbjct: 16  QHTATVIFVHGLGDTGHGWASAVENWRRRQKMDEVKFILPHAPQIPISVNMGMRMPGWFD 75

Query: 81  VEDLSED-----GPDDLEGLDASAAHVAYLLSTE-PSDI---KLAVGGFSMGAATALYSA 140
           ++ L  D       +D EG+  S  +   L+  E  S I   ++ +GGFS G A +L + 
Sbjct: 76  IKQLGGDVDSLIRNEDTEGIKLSQKYFHNLIQQEIDSGIVPERIVLGGFSQGGAMSLLAG 135

Query: 141 -TCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGK 200
            TC S             L  ++GLS WL  SKT    ++   A+       PV++ HG+
Sbjct: 136 LTCTS------------KLGGILGLSSWLLLSKTFADMVKPTDANR----QTPVMMFHGE 195

Query: 201 VDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKL 240
            D +V  + G+ S++ L+  G+ DV +K+Y  +GH  VPEE+DEV A+L  +L
Sbjct: 196 EDPIVPCERGKLSAELLKGLGY-DVAWKTYPGMGHSAVPEELDEVEAFLRKQL 231

BLAST of Carg12144 vs. ExPASy Swiss-Prot
Match: P0CL95 (Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CNBF2260 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.9e-27
Identity = 77/245 (31.43%), Postives = 120/245 (48.98%), Query Frame = 0

Query: 16  VRPKGRHQATVVWLHGLGDNGASWSQLLETL--PLPNIKWICPTAPTRPIALFGGFPSTA 75
           + PK  H ATV++LHGLGD+G  W  + + L    PN+KWI P APT P++L  G    +
Sbjct: 10  ISPKEAHTATVIFLHGLGDSGHGWLPVAKMLWSSFPNVKWILPHAPTIPVSLNHGMAMPS 69

Query: 76  WFDV---EDLSEDGPDDLEGLDASAAHVAYLLSTEPS----DIKLAVGGFSMGAATALYS 135
           WFD+   + L     DD +G+  +   V  L+  E      + ++ +GGFS G A ++  
Sbjct: 70  WFDIRHLDKLDNSENDDEQGMLETLKSVDELIQAEVDSGIPENRIVLGGFSQGGAISVL- 129

Query: 136 ATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGK 195
                     N       L+ VV LS W+P +  +   M E       A  +PV   HG 
Sbjct: 130 ----------NMLTTKRKLAGVVALSTWVPLNHKIVQMMSE------HAKDIPVFWGHGT 189

Query: 196 VDDVVLYKFGEKSSQAL-RSCGFQ-----------DVTFKSYNTLGHYTVPEEMDEVCAW 240
            D VV Y+FG++S   L + CG++            + F+SY  + H + P+E++++ +W
Sbjct: 190 NDPVVDYRFGQRSVDFLVQKCGYKLLSQGTTFARPGIRFESYPGMPHSSCPQEIEDLKSW 237

BLAST of Carg12144 vs. ExPASy TrEMBL
Match: A0A6J1EBV8 (acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432778 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 1.8e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 12  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 71

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 72  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 131

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 132 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 191

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 192 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 251

Query: 241 L 242
           L
Sbjct: 252 L 252

BLAST of Carg12144 vs. ExPASy TrEMBL
Match: A0A6J1KTX4 (acyl-protein thioesterase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497590 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.1e-138
Identity = 240/241 (99.59%), Postives = 240/241 (99.59%), Query Frame = 0

Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 12  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 71

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 72  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 131

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKM EMKASNSGAGSLPV
Sbjct: 132 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMVEMKASNSGAGSLPV 191

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 192 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 251

Query: 241 L 242
           L
Sbjct: 252 L 252

BLAST of Carg12144 vs. ExPASy TrEMBL
Match: A0A6J1KYR2 (acyl-protein thioesterase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497590 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.1e-138
Identity = 240/241 (99.59%), Postives = 240/241 (99.59%), Query Frame = 0

Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 36  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 95

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 96  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 155

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKM EMKASNSGAGSLPV
Sbjct: 156 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMVEMKASNSGAGSLPV 215

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 216 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 275

Query: 241 L 242
           L
Sbjct: 276 L 276

BLAST of Carg12144 vs. ExPASy TrEMBL
Match: A0A0A0LU24 (Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G305760 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.8e-129
Identity = 219/240 (91.25%), Postives = 233/240 (97.08%), Query Frame = 0

Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           + A+KAIEFGKT+VVRPKG+HQATVVWLHGLGDNG+SWSQLLETLPLPNIKWICPTAPTR
Sbjct: 13  RAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPTR 72

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEP+DIKL VGGFSMGAA
Sbjct: 73  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAA 132

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           TALYSATCH++GKYGNGNPYPANLSAVVGLSGWLPCSKTLK+ ME+  A NSGAGSLP+L
Sbjct: 133 TALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPIL 192

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           LCHGKVDDVVLYKFGEKSS+ALRS GF+DVTFKSYN+LGHYTVPEEMDEVCAWLTSKLGL
Sbjct: 193 LCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Carg12144 vs. ExPASy TrEMBL
Match: A0A6J1JJZ2 (acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 3.7e-129
Identity = 221/240 (92.08%), Postives = 230/240 (95.83%), Query Frame = 0

Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           K AR+AIEFGKT+VVRPKG+HQAT+VWLHGLGDNG+SWSQLLE LPLPNIKWICPTAPTR
Sbjct: 13  KAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSSWSQLLEMLPLPNIKWICPTAPTR 72

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PIALFGGFPSTAWFDV+DLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKL VGGFSMGAA
Sbjct: 73  PIALFGGFPSTAWFDVKDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLGVGGFSMGAA 132

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           TALYSATCH++GKYGNGNPYP NLSAVVGLSGWLPCSKTLKSKMEE  ASNSGAGSLP+L
Sbjct: 133 TALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPCSKTLKSKMEEKNASNSGAGSLPIL 192

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           LCHGKVDDVVLYKFGEKSSQAL S GFQDV FKSYN LGHYTVPEEMDEVCAWLTSKLGL
Sbjct: 193 LCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYNGLGHYTVPEEMDEVCAWLTSKLGL 252

BLAST of Carg12144 vs. TAIR 10
Match: AT5G20060.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.1e-112
Identity = 182/240 (75.83%), Postives = 214/240 (89.17%), Query Frame = 0

Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           +  R+A+EFGKTHVVRPKG+HQAT+VWLHGLGDNG+SWSQLLETLPLPNIKWICPTAP++
Sbjct: 12  RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQ 71

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EP+DIKL VGGFSMGAA
Sbjct: 72  PISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAA 131

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           T+LYSATC +LGKYGNGNPYP NLSA++GLSGWLPC+KTL  K+EE +  N  A SLP++
Sbjct: 132 TSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQIKNR-AASLPIV 191

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           +CHGK DDVV +KFGEKSSQAL S GF+ VTFK Y+ LGH+T+P+E+DE+CAWLTS L L
Sbjct: 192 VCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

BLAST of Carg12144 vs. TAIR 10
Match: AT5G20060.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.1e-112
Identity = 182/240 (75.83%), Postives = 214/240 (89.17%), Query Frame = 0

Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           +  R+A+EFGKTHVVRPKG+HQAT+VWLHGLGDNG+SWSQLLETLPLPNIKWICPTAP++
Sbjct: 12  RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQ 71

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EP+DIKL VGGFSMGAA
Sbjct: 72  PISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAA 131

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           T+LYSATC +LGKYGNGNPYP NLSA++GLSGWLPC+KTL  K+EE +  N  A SLP++
Sbjct: 132 TSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQIKNR-AASLPIV 191

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           +CHGK DDVV +KFGEKSSQAL S GF+ VTFK Y+ LGH+T+P+E+DE+CAWLTS L L
Sbjct: 192 VCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

BLAST of Carg12144 vs. TAIR 10
Match: AT5G20060.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.1e-112
Identity = 182/240 (75.83%), Postives = 214/240 (89.17%), Query Frame = 0

Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           +  R+A+EFGKTHVVRPKG+HQAT+VWLHGLGDNG+SWSQLLETLPLPNIKWICPTAP++
Sbjct: 12  RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQ 71

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EP+DIKL VGGFSMGAA
Sbjct: 72  PISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAA 131

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           T+LYSATC +LGKYGNGNPYP NLSA++GLSGWLPC+KTL  K+EE +  N  A SLP++
Sbjct: 132 TSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQIKNR-AASLPIV 191

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           +CHGK DDVV +KFGEKSSQAL S GF+ VTFK Y+ LGH+T+P+E+DE+CAWLTS L L
Sbjct: 192 VCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

BLAST of Carg12144 vs. TAIR 10
Match: AT3G15650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 1.9e-93
Identity = 155/236 (65.68%), Postives = 189/236 (80.08%), Query Frame = 0

Query: 6   KAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIAL 65
           +  EFG+T+VVRPKG+HQAT+VWLHGLGDNG+S SQLLE+LPLPNIKWICPTAP+RP++L
Sbjct: 16  RGYEFGRTYVVRPKGKHQATIVWLHGLGDNGSSSSQLLESLPLPNIKWICPTAPSRPVSL 75

Query: 66  FGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAATALY 125
            GGFP TAWFDV ++SED  DD+EGLDASAAH+A LLS EP+D+K+ +GGFSMGAA ALY
Sbjct: 76  LGGFPCTAWFDVGEISEDLHDDIEGLDASAAHIANLLSAEPTDVKVGIGGFSMGAAIALY 135

Query: 126 SATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHG 185
           S TC++LG+YG G+ Y  NL A VGLSGWLP  ++L+SK+E        A S+P+LL HG
Sbjct: 136 STTCYALGRYGTGHAYTINLRATVGLSGWLPGWRSLRSKIESSNEVARRAASIPILLAHG 195

Query: 186 KVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 242
             DDVV Y+FGEKS+ +L   GF+   FK Y  LGHYTVP+EMDEV  WL S+LGL
Sbjct: 196 TSDDVVPYRFGEKSAHSLAMAGFRQTMFKPYEGLGHYTVPKEMDEVVHWLVSRLGL 251

BLAST of Carg12144 vs. TAIR 10
Match: AT1G52700.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 3.5e-92
Identity = 155/236 (65.68%), Postives = 191/236 (80.93%), Query Frame = 0

Query: 6   KAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIAL 65
           +  EFG+T+VVRPKG+HQAT+VWLHGLGDNG+S SQL+++L LPNIKWICPTAP+RP+  
Sbjct: 16  RGYEFGRTYVVRPKGKHQATLVWLHGLGDNGSSSSQLMDSLHLPNIKWICPTAPSRPVTS 75

Query: 66  FGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAATALY 125
            GGF  TAWFDV ++SEDG DDLEGLDASA+H+A LLS+EP+D+K+ +GGFSMGAA +LY
Sbjct: 76  LGGFTCTAWFDVGEISEDGHDDLEGLDASASHIANLLSSEPADVKVGIGGFSMGAAISLY 135

Query: 126 SATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHG 185
           SATC++LG+YG G+ YP NL AVVGLSGWLP  K+L+SK+E    +   A SLP++L HG
Sbjct: 136 SATCYALGRYGTGHAYPINLQAVVGLSGWLPGWKSLRSKIECSFEAARRAASLPIILTHG 195

Query: 186 KVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 242
             DDVV Y+FGEKS+Q+L   GF+   FK Y  LGHYTVP EMDEV  WLT+ LGL
Sbjct: 196 TSDDVVPYRFGEKSAQSLGMAGFRLAMFKPYEGLGHYTVPREMDEVVHWLTTMLGL 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021798.13.6e-139100.00Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022925495.13.6e-139100.00acyl-protein thioesterase 2-like [Cucurbita moschata] >XP_022925502.1 acyl-prote... [more]
KAG6587912.13.6e-139100.00Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023530533.13.6e-139100.00acyl-protein thioesterase 2-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023004193.12.3e-13899.59acyl-protein thioesterase 2-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O953725.2e-3236.44Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1[more]
Q9WTL71.2e-3136.00Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1[more]
Q9QYL81.2e-3136.00Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1[more]
Q4I8Q42.3e-2733.91Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 12365... [more]
P0CL953.9e-2731.43Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype ... [more]
Match NameE-valueIdentityDescription
A0A6J1EBV81.8e-139100.00acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432778 P... [more]
A0A6J1KTX41.1e-13899.59acyl-protein thioesterase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1KYR21.1e-13899.59acyl-protein thioesterase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0LU242.8e-12991.25Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G3057... [more]
A0A6J1JJZ23.7e-12992.08acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=... [more]
Match NameE-valueIdentityDescription
AT5G20060.21.1e-11275.83alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.11.1e-11275.83alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.31.1e-11275.83alpha/beta-Hydrolases superfamily protein [more]
AT3G15650.11.9e-9365.68alpha/beta-Hydrolases superfamily protein [more]
AT1G52700.13.5e-9265.68alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003140Phospholipase/carboxylesterase/thioesterasePFAMPF02230Abhydrolase_2coord: 13..235
e-value: 5.5E-40
score: 137.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 3..240
e-value: 3.1E-63
score: 215.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 14..239
NoneNo IPR availablePANTHERPTHR46234:SF9ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 2..241
NoneNo IPR availablePANTHERPTHR46234ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 2..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12144-RACarg12144-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity