CSPI06G33720 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G33720
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pectinesterase
Location	Chr6: 27938870 .. 27940986 (-)
RNA-Seq Expression	CSPI06G33720
Synteny	CSPI06G33720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATCATATCGCAAAAGAATCCAATAAATGAAACTTTGATGGTCCCATTTTTGTAATGGGGCTTTACTCTATTATTATCCTATTTAAATCTCTAGTGGGTGAAGCTTAATTCATCACTTGAAGCTTCACTTACAAGCACTAGTGATTGTTCATGGCTACCCATCAGCCACTTCTTCTTCTTCAACCTCCCAAAACTTCCCGCTCCAAAACCATTCCCACACTCCTCTGCTTTGCTGCGGTTTTGTGCTCAGCCATTTTCATTACTAACAAGTTCATCAAACCAAACCCATCTGAAAATGATCTTCCCTTCCTTCAAAACATATGCCACAAGGCTTATGACCCATCATCTTGCATTGAAATGGCCGCCTCAGAATTTCCCTTGAGCATAATCAAAACCACAAACGAGGTTGATTTCTTACAGTCTTTCCTGAGGAAATCCATGCCTAAAGTAATTAGCACAACTGAGAGAGCCAAAGATATCCGCCAAAGAATCAACAGCCCTCGCGGTGAAGCAGCTTTGGCCGATTGTATCGAGCTTATGGAGATTTCAAACGGCCGAATCATGGACTCTGTTTTGGCTCTGAAGAACAGAACGAGCGGCTCCATCGAAAATTCCCACACATGGCTGAGTAGCGTGCTGACAAACCACGTCACGTGCTGGGATGAGGTGGAGAGTTCATTATCCAGAGCGGCGGCAATGGATTTAGGGCTCGAAGAGTTGATTATGAGAGGGAGAAACTCGTTGGGGATGTTGGTTTCGATTTGGGGACTGGACATTAAAAATCTGGGAGAGTTGGAGAAAAAGGGAAATGGGTATCCGAGTTGGTTGAAGAAAGGAGATCGGAGATTATTGGGAGTTTTGGGGAGAGAAATGGAACCGAATATCGTGGTGGCGAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAATCAGTTCCAGATAAAAGCAAGAACAGAATTGTTATATACGTGAAAAGGGGGACTTATGAGGAAAATGTGGAAGTGGGAAAGAAAAAAAAGAATGTAATGATCGTTGGAGATGGAATGGATTCCACCATTATCACCGGTAGCCTTAACGTCGTTGATGGATCAACAACTTTCAAATCTGCAACTGTAGGTAAGACTATCAGAAACACTGGTTCCTCTTTCTCTCCTAATCTTTTCATCACTTATGATCAACAACTTTCTTGTTTGATTTGATGATCCAGCTGCGGTGGGAGATGGGTTTATAGCCCAAGACATTTGGTTCCAAAACACCGCCGGGCCGGAAAAGCACCAAGCCGTGGCGCTTCGGGTCGGAGCAGATCAATCGGTCATAAACCGGTGTCGTATAGACGCTTATCAGGACACACTCTACACACATTCCAACCGCCAATTCTACCGAGATTCCACCATCACTGGCACCGTCGACTTCATATTCGGTAACGCTGCCGTCGTTTTACAGAACTGTAAAATAGAGCCAAGACGACCGATGAACAACCAAGCCAACATGGTGACGGCGCAAGGCCGAATCGACCCAAATCAGAACACTGGAACTTCGATCCAGCAATGCGACATCGTGGCAAGCTCCGACTTGGAGCCGGTTAAGAAGTCGATTAAGACATATCTGGGGCGACCATGGAAGGAGTATTCGAGGACCGTCATCATGCAGTCGAGAATTGGCGATCTTATTCAACCAGCGGGCTGGGCCGAATGGAATGGGGACTTTGCACTGAAAACGCTGTATTACGGGGAGTATTCAAATACTGGGCCGGGCTCGGATGTTAGCAAGCGAGTGAAGTGGGACGGTTATCATGTCATTACGAGCCCATCTGAGGCCCAAAAGTTTACGGTCGATTCACTTATACAAGGAGGTGAATGGCTGGGCCCATCGGGCGCGACTTTTATCCCAGGGCTGTGATTGTGTGCGCTTGTCATCAACGGTTGAGATCTACTTTTCCTGTCATCGTTGTTTTATTAGGCAATAAAATTGAAGAGAACGAATATATCATCGTTCTATCAATTATAATTACAAAACTAAAATTAGGTTTTGTTTCAACAAAGTTCACATCCCAAGTTTTCTAAATTTTGAAAGAGTTAAAAACAAATGAAATGCTTTAACTTAAAG

mRNA sequence

CAATCATATCGCAAAAGAATCCAATAAATGAAACTTTGATGGTCCCATTTTTGTAATGGGGCTTTACTCTATTATTATCCTATTTAAATCTCTAGTGGGTGAAGCTTAATTCATCACTTGAAGCTTCACTTACAAGCACTAGTGATTGTTCATGGCTACCCATCAGCCACTTCTTCTTCTTCAACCTCCCAAAACTTCCCGCTCCAAAACCATTCCCACACTCCTCTGCTTTGCTGCGGTTTTGTGCTCAGCCATTTTCATTACTAACAAGTTCATCAAACCAAACCCATCTGAAAATGATCTTCCCTTCCTTCAAAACATATGCCACAAGGCTTATGACCCATCATCTTGCATTGAAATGGCCGCCTCAGAATTTCCCTTGAGCATAATCAAAACCACAAACGAGGTTGATTTCTTACAGTCTTTCCTGAGGAAATCCATGCCTAAAGTAATTAGCACAACTGAGAGAGCCAAAGATATCCGCCAAAGAATCAACAGCCCTCGCGGTGAAGCAGCTTTGGCCGATTGTATCGAGCTTATGGAGATTTCAAACGGCCGAATCATGGACTCTGTTTTGGCTCTGAAGAACAGAACGAGCGGCTCCATCGAAAATTCCCACACATGGCTGAGTAGCGTGCTGACAAACCACGTCACGTGCTGGGATGAGGTGGAGAGTTCATTATCCAGAGCGGCGGCAATGGATTTAGGGCTCGAAGAGTTGATTATGAGAGGGAGAAACTCGTTGGGGATGTTGGTTTCGATTTGGGGACTGGACATTAAAAATCTGGGAGAGTTGGAGAAAAAGGGAAATGGGTATCCGAGTTGGTTGAAGAAAGGAGATCGGAGATTATTGGGAGTTTTGGGGAGAGAAATGGAACCGAATATCGTGGTGGCGAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAATCAGTTCCAGATAAAAGCAAGAACAGAATTGTTATATACGTGAAAAGGGGGACTTATGAGGAAAATGTGGAAGTGGGAAAGAAAAAAAAGAATGTAATGATCGTTGGAGATGGAATGGATTCCACCATTATCACCGGTAGCCTTAACGTCGTTGATGGATCAACAACTTTCAAATCTGCAACTGTAGCTGCGGTGGGAGATGGGTTTATAGCCCAAGACATTTGGTTCCAAAACACCGCCGGGCCGGAAAAGCACCAAGCCGTGGCGCTTCGGGTCGGAGCAGATCAATCGGTCATAAACCGGTGTCGTATAGACGCTTATCAGGACACACTCTACACACATTCCAACCGCCAATTCTACCGAGATTCCACCATCACTGGCACCGTCGACTTCATATTCGGTAACGCTGCCGTCGTTTTACAGAACTGTAAAATAGAGCCAAGACGACCGATGAACAACCAAGCCAACATGGTGACGGCGCAAGGCCGAATCGACCCAAATCAGAACACTGGAACTTCGATCCAGCAATGCGACATCGTGGCAAGCTCCGACTTGGAGCCGGTTAAGAAGTCGATTAAGACATATCTGGGGCGACCATGGAAGGAGTATTCGAGGACCGTCATCATGCAGTCGAGAATTGGCGATCTTATTCAACCAGCGGGCTGGGCCGAATGGAATGGGGACTTTGCACTGAAAACGCTGTATTACGGGGAGTATTCAAATACTGGGCCGGGCTCGGATGTTAGCAAGCGAGTGAAGTGGGACGGTTATCATGTCATTACGAGCCCATCTGAGGCCCAAAAGTTTACGGTCGATTCACTTATACAAGGAGGTGAATGGCTGGGCCCATCGGGCGCGACTTTTATCCCAGGGCTGTGATTGTGTGCGCTTGTCATCAACGGTTGAGATCTACTTTTCCTGTCATCGTTGTTTTATTAGGCAATAAAATTGAAGAGAACGAATATATCATCGTTCTATCAATTATAATTACAAAACTAAAATTAGGTTTTGTTTCAACAAAGTTCACATCCCAAGTTTTCTAAATTTTGAAAGAGTTAAAAACAAATGAAATGCTTTAACTTAAAG

Coding sequence (CDS)

ATGGCTACCCATCAGCCACTTCTTCTTCTTCAACCTCCCAAAACTTCCCGCTCCAAAACCATTCCCACACTCCTCTGCTTTGCTGCGGTTTTGTGCTCAGCCATTTTCATTACTAACAAGTTCATCAAACCAAACCCATCTGAAAATGATCTTCCCTTCCTTCAAAACATATGCCACAAGGCTTATGACCCATCATCTTGCATTGAAATGGCCGCCTCAGAATTTCCCTTGAGCATAATCAAAACCACAAACGAGGTTGATTTCTTACAGTCTTTCCTGAGGAAATCCATGCCTAAAGTAATTAGCACAACTGAGAGAGCCAAAGATATCCGCCAAAGAATCAACAGCCCTCGCGGTGAAGCAGCTTTGGCCGATTGTATCGAGCTTATGGAGATTTCAAACGGCCGAATCATGGACTCTGTTTTGGCTCTGAAGAACAGAACGAGCGGCTCCATCGAAAATTCCCACACATGGCTGAGTAGCGTGCTGACAAACCACGTCACGTGCTGGGATGAGGTGGAGAGTTCATTATCCAGAGCGGCGGCAATGGATTTAGGGCTCGAAGAGTTGATTATGAGAGGGAGAAACTCGTTGGGGATGTTGGTTTCGATTTGGGGACTGGACATTAAAAATCTGGGAGAGTTGGAGAAAAAGGGAAATGGGTATCCGAGTTGGTTGAAGAAAGGAGATCGGAGATTATTGGGAGTTTTGGGGAGAGAAATGGAACCGAATATCGTGGTGGCGAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAATCAGTTCCAGATAAAAGCAAGAACAGAATTGTTATATACGTGAAAAGGGGGACTTATGAGGAAAATGTGGAAGTGGGAAAGAAAAAAAAGAATGTAATGATCGTTGGAGATGGAATGGATTCCACCATTATCACCGGTAGCCTTAACGTCGTTGATGGATCAACAACTTTCAAATCTGCAACTGTAGCTGCGGTGGGAGATGGGTTTATAGCCCAAGACATTTGGTTCCAAAACACCGCCGGGCCGGAAAAGCACCAAGCCGTGGCGCTTCGGGTCGGAGCAGATCAATCGGTCATAAACCGGTGTCGTATAGACGCTTATCAGGACACACTCTACACACATTCCAACCGCCAATTCTACCGAGATTCCACCATCACTGGCACCGTCGACTTCATATTCGGTAACGCTGCCGTCGTTTTACAGAACTGTAAAATAGAGCCAAGACGACCGATGAACAACCAAGCCAACATGGTGACGGCGCAAGGCCGAATCGACCCAAATCAGAACACTGGAACTTCGATCCAGCAATGCGACATCGTGGCAAGCTCCGACTTGGAGCCGGTTAAGAAGTCGATTAAGACATATCTGGGGCGACCATGGAAGGAGTATTCGAGGACCGTCATCATGCAGTCGAGAATTGGCGATCTTATTCAACCAGCGGGCTGGGCCGAATGGAATGGGGACTTTGCACTGAAAACGCTGTATTACGGGGAGTATTCAAATACTGGGCCGGGCTCGGATGTTAGCAAGCGAGTGAAGTGGGACGGTTATCATGTCATTACGAGCCCATCTGAGGCCCAAAAGTTTACGGTCGATTCACTTATACAAGGAGGTGAATGGCTGGGCCCATCGGGCGCGACTTTTATCCCAGGGCTGTGA

Protein sequence

MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHKAYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQGGEWLGPSGATFIPGL*

Homology

[Close]

BLAST of CSPI06G33720 vs. ExPASy Swiss-Prot
Match: P83947 (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=204231 PE=1 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 7.3e-170
Identity = 311/559 (55.64%), Postives = 394/559 (70.48%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           M  +QP  LL+  K+  SK    +  F  V+  A  ++  F  P     +L     IC +
Sbjct: 1   MEINQP-NLLEASKSCYSK----ITFFLLVISFAALVSTGFSSP-----ELSLHHKICDQ 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 120
           + +  SC+ M +    L++    N    L+SFL K+ P++    E A D  +RIN+P+  
Sbjct: 61  SVNKESCLAMISEVTGLNMADHRN---LLKSFLEKTTPRIQKAFETANDASRRINNPQER 120

Query: 121 AALADCIELMEISNGRIMDSVLAL--KNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLS 180
            AL DC ELM++S  R++DS+  L  +N T+ S E+ H WLS VLTNHVTC D +E   +
Sbjct: 121 TALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLEEGST 180

Query: 181 R--AAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGV 240
                 M+  L ELI+R R SL + V+++         +E     +P+W+  GDRRLL  
Sbjct: 181 DYIKTLMESHLNELILRARTSLAIFVTLFP---AKSNVIEPVTGNFPTWVTAGDRRLLQT 240

Query: 241 LGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVM 300
           LG+++EP+IVVAKDGSG+++T+ EAV ++PD SK R+++ V+ G YEENV+ G +KKNVM
Sbjct: 241 LGKDIEPDIVVAKDGSGDYETLNEAVAAIPDNSKKRVIVLVRTGIYEENVDFGYQKKNVM 300

Query: 301 IVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVG 360
           +VG+GMD TIITGS NVVDGSTTF SATVAAVGDGFIAQDI FQNTAGPEK+QAVALR+G
Sbjct: 301 LVGEGMDYTIITGSRNVVDGSTTFDSATVAAVGDGFIAQDICFQNTAGPEKYQAVALRIG 360

Query: 361 ADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNN 420
           AD++VINRCRIDAYQDTLY H+ RQFYRD  ITGTVDFIFGNAAVV QNC + PR+ M  
Sbjct: 361 ADETVINRCRIDAYQDTLYPHNYRQFYRDRNITGTVDFIFGNAAVVFQNCNLIPRKQMKG 420

Query: 421 QANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRI 480
           Q N +TAQGR DPNQNTGTSIQ C+I AS+DLEPV+ + K+YLGRPWKEYSRTV+M+S I
Sbjct: 421 QENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESYI 480

Query: 481 GDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDS 540
            D+I PAGW EW+ DFALKTL+YGEY N GPGS  S+RVKW GYHVITSP  A++FTV  
Sbjct: 481 SDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAE 540

Query: 541 LIQGGEWLGPSGATFIPGL 556
           LIQGG WLG +G  +  GL
Sbjct: 541 LIQGGSWLGSTGVDYTAGL 543

[Close]

BLAST of CSPI06G33720 vs. ExPASy Swiss-Prot
Match: Q1JPL7 (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PME18 PE=1 SV=3)

HSP 1 Score: 577.8 bits (1488), Expect = 1.3e-163
Identity = 310/558 (55.56%), Postives = 386/558 (69.18%), Query Frame = 0


Query: 3   THQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFL--QNICHK 62
           ++QP  LL  PK+ + K +  +L F A+L S  F T + I  N + ND   L    ICH 
Sbjct: 4   SNQP--LLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHG 63

Query: 63  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 122
           A+D  SC +   SEF    +   N +D L  FL+ S+ ++ ST     + R R N  R +
Sbjct: 64  AHDQDSC-QALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDK 123

Query: 123 AALADCIELMEISNGRIMDSVLALK--NRTSGSIENSHTWLSSVLTNHVTCWDEV-ESSL 182
           A  ADC E+M++S  R+M S+  L+  N    S  N HTWLSSVLTN++TC + + + S+
Sbjct: 124 AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSV 183

Query: 183 SRAAAMDLGLEELIMRGRNSLGMLVSIWGL--DIKNLGELEKKGNGYPSWLKKGDRRLLG 242
           +    +   LE+L+ R R +L + VS+     D+K +       N +PSWL   DR+LL 
Sbjct: 184 NSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMI-----ISNRFPSWLTALDRKLLE 243

Query: 243 VLGREME--PNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKK 302
              + ++   N+VVAKDG+G FKTV EAV + P+ S  R VIYVK+G Y+E +++GKKKK
Sbjct: 244 SSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKK 303

Query: 303 NVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVAL 362
           N+M+VGDG D+TIITGSLNV+DGSTTF+SATVAA GDGF+AQDIWFQNTAGP KHQAVAL
Sbjct: 304 NLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVAL 363

Query: 363 RVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRP 422
           RV ADQ+VINRCRIDAYQDTLYTH+ RQFYRDS ITGTVDFIFGN+AVV QNC I  R P
Sbjct: 364 RVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNP 423

Query: 423 MNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQ 482
              Q NM+TAQGR D NQNT  SIQ+C I ASSDL PVK S+KT+LGRPWK YSRTVIMQ
Sbjct: 424 GAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQ 483

Query: 483 SRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFT 542
           S I + I PAGW  W+G+FAL TLYYGEY+NTGPG+D SKRV W G+ VI    EA++FT
Sbjct: 484 SFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFT 543

Query: 543 VDSLIQGGEWLGPSGATF 552
           V  LIQGG WL P+G TF
Sbjct: 544 VAKLIQGGLWLKPTGVTF 553

[Close]

BLAST of CSPI06G33720 vs. ExPASy Swiss-Prot
Match: P09607 (Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2)

HSP 1 Score: 568.2 bits (1463), Expect = 1.1e-160
Identity = 301/559 (53.85%), Postives = 383/559 (68.52%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPT--LLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNIC 60
           MAT Q  LL    KT +  +I +  +L F   L  A+F+    + P   E       N+C
Sbjct: 1   MATPQQPLL---TKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIK---HSNLC 60

Query: 61  HKAYDPSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRKSMPKVISTTERAKDIRQRINSP 120
             A D   C+   +      I+ + ++ +  L+ FL  S+ ++ +     + I+ +IN  
Sbjct: 61  KTAQDSQLCLSYVSDLISNEIVTSDSDGLSILKKFLVYSVHQMNNAIPVVRKIKNQINDI 120

Query: 121 RGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSL 180
           R + AL DC+EL+++S   + DS+ A+  R+     N+ +WLS VLTNHVTC DE++ S 
Sbjct: 121 REQGALTDCLELLDLSVDLVCDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SF 180

Query: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNG-YPSWLKKGDRRLLGV 240
           ++A      L+ELI R + +L ML S+   +     E+ + G G  PSW+   DR+L+  
Sbjct: 181 TKAMINGTNLDELISRAKVALAMLASVTTPN----DEVLRPGLGKMPSWVSSRDRKLMES 240

Query: 241 LGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVM 300
            G+++  N VVAKDG+G ++T+ EAV + PDKSK R VIYVKRGTY+ENVEV  +K N+M
Sbjct: 241 SGKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVSSRKMNLM 300

Query: 301 IVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVG 360
           I+GDGM +TIITGSLNVVDGSTTF SAT+AAVG GFI QDI  QNTAGP KHQAVALRVG
Sbjct: 301 IIGDGMYATIITGSLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVG 360

Query: 361 ADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNN 420
           AD+SVINRCRIDAYQDTLY HS RQFYRDS +TGT+DFIFGNAAVV Q C++  R+P   
Sbjct: 361 ADKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCQLVARKPGKY 420

Query: 421 QANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRI 480
           Q NMVTAQGR DPNQ TGTSIQ CDI+AS DL+PV K   TYLGRPWK+YSRTV+M+S +
Sbjct: 421 QQNMVTAQGRTDPNQATGTSIQFCDIIASPDLKPVVKEFPTYLGRPWKKYSRTVVMESYL 480

Query: 481 GDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDS 540
           G LI P+GWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYHVIT P+EA  FTV  
Sbjct: 481 GGLIDPSGWAEWHGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAEAMSFTVAK 540

Query: 541 LIQGGEWLGPSGATFIPGL 556
           LIQGG WL  +   ++ GL
Sbjct: 541 LIQGGSWLRSTDVAYVDGL 548

[Close]

BLAST of CSPI06G33720 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 4.0e-160
Identity = 302/558 (54.12%), Postives = 374/558 (67.03%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPT--LLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNIC 60
           MAT Q  LL    KT +  +I +  +L F   L  A+F+    + P   E       N+C
Sbjct: 1   MATPQQPLL---TKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIK---HSNLC 60

Query: 61  HKAYDPSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRKSMPKVISTTERAKDIRQRINSP 120
             A D   C+   +      I+ T ++ +  L  FL   + ++ +       ++ +IN  
Sbjct: 61  KTAQDSQLCLSYVSDLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDI 120

Query: 121 RGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSL 180
           R E AL DC+EL++ S   + DS+ A+  RT     N+ +WLS VLTNHVTC DE++ S 
Sbjct: 121 RQEGALTDCLELLDQSVDLVSDSIAAIDKRTHSEHANAQSWLSGVLTNHVTCLDELD-SF 180

Query: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVL 240
           ++A      L+ELI R + +L ML S+      N   L       PSW+   DR+L+   
Sbjct: 181 TKAMINGTNLDELISRAKVALAMLASV---TTPNDDVLRPGLGKMPSWVSSRDRKLMESS 240

Query: 241 GREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMI 300
           G+++  N VVAKDG+G ++T+ EAV + PDKSK R VIYVKRG Y+ENVEV  +K  +MI
Sbjct: 241 GKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMI 300

Query: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 360
           VGDGM +TIITG+LNVVDGSTTF SAT+AAVG GFI QDI  QNTAGP KHQAVALRVGA
Sbjct: 301 VGDGMHATIITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGA 360

Query: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 420
           D+SVINRCRIDAYQDTLY HS RQFYRDS +TGT+DFIFGNAAVV Q CK+  R+P   Q
Sbjct: 361 DKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQ 420

Query: 421 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIG 480
            NMVTAQGR DPNQ TGTSIQ C+I+ASSDLEPV K   TYLGRPWK+YSRTV+M+S +G
Sbjct: 421 QNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLG 480

Query: 481 DLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSL 540
            LI PAGWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYH IT P+EA  FTV  L
Sbjct: 481 GLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKL 540

Query: 541 IQGGEWLGPSGATFIPGL 556
           IQGG WL  +G  ++ GL
Sbjct: 541 IQGGSWLRSTGVAYVDGL 548

[Close]

BLAST of CSPI06G33720 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 555.4 bits (1430), Expect = 7.1e-157
Identity = 300/558 (53.76%), Postives = 376/558 (67.38%), Query Frame = 0


Query: 5   QPLLLLQPPKTSRSKTIPT--LLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHKAY 64
           QPLL+    KT +   I +  +L F   L  A+F+        P + ++    N+C  A 
Sbjct: 6   QPLLI----KTHKQNPIISFKILSFVITLFVALFLV------APYQVEIKH-SNLCKTAQ 65

Query: 65  DPSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGEA 124
           D   C+   +      I+ T ++    L  FL   + ++ +     + ++ +IN  R   
Sbjct: 66  DSQLCLSYVSDLISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHG 125

Query: 125 ALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRAA 184
           AL DC+EL++ S     DS+ A+  R+     N+ +WLS VLTNHVTC DE++ S ++A 
Sbjct: 126 ALTDCLELLDQSVDFASDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKAM 185

Query: 185 AMDLGLEELIMRGRNSLGMLVSIWGLD----IKNLGELEKKGNGYPSWLKKGDRRLLGVL 244
                LEELI R + +L ML S+   D    +  LG++       PSW+   DR+L+   
Sbjct: 186 INGTNLEELISRAKVALAMLASLTTQDEDVFMTVLGKM-------PSWVSSMDRKLMESS 245

Query: 245 GREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMI 304
           G+++  N VVA+DG+G+++T+ EAV + PDKSK R VIYVKRGTY+ENVEV   K N+MI
Sbjct: 246 GKDIIANAVVAQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMI 305

Query: 305 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 364
           VGDGM +T ITGSLNVVDGSTTF+SAT+AAVG GFI QDI  QNTAGP K QAVALRVGA
Sbjct: 306 VGDGMYATTITGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGA 365

Query: 365 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 424
           D SVINRCRIDAYQDTLY HS RQFYRDS +TGTVDFIFGNAAVV Q C++  R+P   Q
Sbjct: 366 DMSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQ 425

Query: 425 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIG 484
            NMVTAQGR DPNQ TGTSIQ C+I+ASSDLEPV K   TYLGRPWKEYSRTV+M+S +G
Sbjct: 426 QNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLG 485

Query: 485 DLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSL 544
            LI PAGWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYHVIT P++A  FTV  L
Sbjct: 486 GLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKL 544

Query: 545 IQGGEWLGPSGATFIPGL 556
           IQGG WL  +G  ++ GL
Sbjct: 546 IQGGSWLRSTGVAYVDGL 544

[Close]

BLAST of CSPI06G33720 vs. ExPASy TrEMBL
Match: A0A0A0KHV5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 552/555 (99.46%), Postives = 553/555 (99.64%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 120
           AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVIST ERAKDIRQRINSPRGE
Sbjct: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
           AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA
Sbjct: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           A MDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 APMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQG 540
           QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYH+ITSPSEAQKFTVDSLIQG
Sbjct: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGATFIPGL
Sbjct: 541 GEWLGPSGATFIPGL 555

[Close]

BLAST of CSPI06G33720 vs. ExPASy TrEMBL
Match: A0A5D3CNY5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006640 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 522/555 (94.05%), Postives = 540/555 (97.30%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI T+LCFA VLCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 120
           AY+PSSCIEMAASEFPLSIIKTTNE  FLQSFL+KSMPKVI+T ERAKD+RQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIMDSVLALKNRTSGSIE+SHTWLSSVLTNHVTCWDEVE+SLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELIMRGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSK+RIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKW GYHVITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

[Close]

BLAST of CSPI06G33720 vs. ExPASy TrEMBL
Match: A0A1S3B018 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.4e-303
Identity = 522/555 (94.05%), Postives = 538/555 (96.94%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI T+LCFA VLCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 120
           AY+PSSCIEMAASEFPLSIIKTTNE  FLQSFL+KSMPKVI+T ERAKD+RQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIMDSVLALKNRTSG IE+SHTWLSSVLTNHVTCWDEVESSLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELIMRGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSKNRIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKW GYHVITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

[Close]

BLAST of CSPI06G33720 vs. ExPASy TrEMBL
Match: A0A6J1KRW2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.1e-253
Identity = 447/557 (80.25%), Postives = 494/557 (88.69%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSEND-LPFLQNICH 60
           MATH+P LL QPP+T+R KT  T+L F+ +L SAIFITNK I  NPSEND  PFL NIC+
Sbjct: 1   MATHEP-LLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 60

Query: 61  KAYDPSSCIEMAASEFP-LSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPR 120
           KAY+ SSCI+M ASEFP L   KTTN  +FLQSFLRKS  K+++T E AKD+R+RIN+PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 121 GEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLS 180
           GEAALADC+ELMEIS  RI DS+LAL+ R  G+IEN+HTWLSSVLTNHV C DE+E+S+S
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 181 RAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLG 240
           +AAAMDLG+EELI+ GRNSLGMLVSIWG         +K+G GYPSWLKKGDRRLL V+G
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 240

Query: 241 REMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIV 300
           R M+ +IVVAKDGSGNFKTVKEAVE+VPDKSK+RIVIYVK+G Y+ENVEVGK KKNVM+V
Sbjct: 241 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 300

Query: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGAD 360
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 360

Query: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 420

Query: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGD 480
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQS +GD
Sbjct: 421 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 480

Query: 481 LIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLI 540
           LI PAGWAEWNG+FALKTLYYGEYSN GPGS+VSKRVKW GYHVITSP+ AQKFTVDSLI
Sbjct: 481 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 540

Query: 541 QGGEWLGPSGATFIPGL 556
           QG EWLG SG TFIPGL
Sbjct: 541 QGAEWLGSSGVTFIPGL 549

[Close]

BLAST of CSPI06G33720 vs. ExPASy TrEMBL
Match: A0A6J1CN37 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 6.7e-243
Identity = 441/561 (78.61%), Postives = 473/561 (84.31%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MAT+QP  LLQPPKT RSK    +LCFA +LCSA FI N  I PNPSE D  FL NIC K
Sbjct: 1   MATNQP--LLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60

Query: 61  AYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTTERAKD--IRQRINSP 120
           AY+P+SC+ MAASE P     K TN V FLQ FLRKS+ KV  T ERA D  + +RIN  
Sbjct: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANDLILSRRINDA 120

Query: 121 RGEAALADCIELMEISNGRIMDSV--LALKNRTSGSIENSHTWLSSVLTNHVTCWDEVES 180
           RGEAALADC+ELMEIS  RI DS+  LAL N+TS  IE++H WLSSVLTNHVTC DE+E 
Sbjct: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSVPIEDAHAWLSSVLTNHVTCSDELEK 180

Query: 181 SLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGN-GYPSWLKKGDRRLL 240
           SL    A +LGLEELIM  RNSL MLVSI     +   E E +G  G+P WLK GDR LL
Sbjct: 181 SLLGTTAAELGLEELIMTARNSLAMLVSI----SQPEPEPEIEGQIGFPGWLKSGDRMLL 240

Query: 241 GVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKN 300
           GV G++++ NIVVAKDGSGNFKTVKEAV SVPDKSKNR VIYVK+GTYEENVEV KKKKN
Sbjct: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGPEKHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420

Query: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVASSDLEPVK+SIKTYLGRPWKEYSRTVIMQS
Sbjct: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480

Query: 481 RIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTV 540
           RIGDLI P GWAEWNGDFALKTLYYGEY N GPGSDVSKRVKW GY VIT+P+EAQKFTV
Sbjct: 481 RIGDLIDPTGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540

Query: 541 DSLIQGGEWLGPSGATFIPGL 556
           DSLIQGG+WLGP+G TFIPGL
Sbjct: 541 DSLIQGGDWLGPTGVTFIPGL 555

[Close]

BLAST of CSPI06G33720 vs. NCBI nr
Match: XP_004134752.1 (pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cucumis sativus])

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 552/555 (99.46%), Postives = 553/555 (99.64%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 120
           AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVIST ERAKDIRQRINSPRGE
Sbjct: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
           AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA
Sbjct: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           A MDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 APMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQG 540
           QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYH+ITSPSEAQKFTVDSLIQG
Sbjct: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGATFIPGL
Sbjct: 541 GEWLGPSGATFIPGL 555

[Close]

BLAST of CSPI06G33720 vs. NCBI nr
Match: TYK13062.1 (pectinesterase [Cucumis melo var. makuwa])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.4e-303
Identity = 522/555 (94.05%), Postives = 540/555 (97.30%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI T+LCFA VLCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 120
           AY+PSSCIEMAASEFPLSIIKTTNE  FLQSFL+KSMPKVI+T ERAKD+RQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIMDSVLALKNRTSGSIE+SHTWLSSVLTNHVTCWDEVE+SLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELIMRGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSK+RIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKW GYHVITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

[Close]

BLAST of CSPI06G33720 vs. NCBI nr
Match: XP_008439965.1 (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.0e-303
Identity = 522/555 (94.05%), Postives = 538/555 (96.94%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI T+LCFA VLCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 120
           AY+PSSCIEMAASEFPLSIIKTTNE  FLQSFL+KSMPKVI+T ERAKD+RQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIMDSVLALKNRTSG IE+SHTWLSSVLTNHVTCWDEVESSLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELIMRGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSKNRIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKW GYHVITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

[Close]

BLAST of CSPI06G33720 vs. NCBI nr
Match: XP_038881670.1 (pectinesterase [Benincasa hispida])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.8e-288
Identity = 498/556 (89.57%), Postives = 526/556 (94.60%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQP  LLQPPKTSRSKTIPT+LCFAAVLCSAIFITNKFIK N SENDL FL N+CHK
Sbjct: 1   MATHQP--LLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKSNSSENDLSFLHNLCHK 60

Query: 61  AYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRG 120
           AY+PSSCIEMAASEFP    IKTTN   FLQSFLRKS+PKVI+T +RAKD+ QRIN+PRG
Sbjct: 61  AYNPSSCIEMAASEFPFPREIKTTNRGHFLQSFLRKSIPKVINTIDRAKDLSQRINNPRG 120

Query: 121 EAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSR 180
           EAALADC+ELMEISN RI+DSV ALKNRTSGSIEN+HTWLSSVLTNH+TCWDEVE+S+SR
Sbjct: 121 EAALADCVELMEISNSRIIDSVSALKNRTSGSIENAHTWLSSVLTNHITCWDEVENSVSR 180

Query: 181 AAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGR 240
           AAAMDLGLEELIMRGRNSLGM VSIW  +I+N+GELEK+ NGYPSWLKKGDRRLLGV G+
Sbjct: 181 AAAMDLGLEELIMRGRNSLGMFVSIWESEIENMGELEKRVNGYPSWLKKGDRRLLGVPGK 240

Query: 241 EMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVG 300
           EME NIVVAKDGSGNF TV EAVESVPDKSKNRIVIYVK+G Y+E VEVGKKKKNVMIVG
Sbjct: 241 EMEANIVVAKDGSGNFMTVTEAVESVPDKSKNRIVIYVKKGIYKEKVEVGKKKKNVMIVG 300

Query: 301 DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ 360
           DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ
Sbjct: 301 DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ 360

Query: 361 SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQAN 420
           SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQAN
Sbjct: 361 SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQAN 420

Query: 421 MVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDL 480
           MVTAQGR+DPNQNTG+SIQ+C+IVASSDLEPVK+SIKTYLGRPWKEYSRTV+MQS IGDL
Sbjct: 421 MVTAQGRMDPNQNTGSSIQKCEIVASSDLEPVKQSIKTYLGRPWKEYSRTVVMQSTIGDL 480

Query: 481 IQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQ 540
           IQPAGWAEWNGDFALKTLYYGEYSN GPGSDVSKRVKW GYHVITSPSEAQKFTVDSLIQ
Sbjct: 481 IQPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWAGYHVITSPSEAQKFTVDSLIQ 540

Query: 541 GGEWLGPSGATFIPGL 556
           GGEWLGPSGATFIPGL
Sbjct: 541 GGEWLGPSGATFIPGL 554

[Close]

BLAST of CSPI06G33720 vs. NCBI nr
Match: XP_023002934.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 885.6 bits (2287), Expect = 2.3e-253
Identity = 447/557 (80.25%), Postives = 494/557 (88.69%), Query Frame = 0


Query: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSEND-LPFLQNICH 60
           MATH+P LL QPP+T+R KT  T+L F+ +L SAIFITNK I  NPSEND  PFL NIC+
Sbjct: 1   MATHEP-LLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 60

Query: 61  KAYDPSSCIEMAASEFP-LSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPR 120
           KAY+ SSCI+M ASEFP L   KTTN  +FLQSFLRKS  K+++T E AKD+R+RIN+PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 121 GEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLS 180
           GEAALADC+ELMEIS  RI DS+LAL+ R  G+IEN+HTWLSSVLTNHV C DE+E+S+S
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 181 RAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLG 240
           +AAAMDLG+EELI+ GRNSLGMLVSIWG         +K+G GYPSWLKKGDRRLL V+G
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 240

Query: 241 REMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIV 300
           R M+ +IVVAKDGSGNFKTVKEAVE+VPDKSK+RIVIYVK+G Y+ENVEVGK KKNVM+V
Sbjct: 241 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 300

Query: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGAD 360
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 360

Query: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 420

Query: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGD 480
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQS +GD
Sbjct: 421 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 480

Query: 481 LIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLI 540
           LI PAGWAEWNG+FALKTLYYGEYSN GPGS+VSKRVKW GYHVITSP+ AQKFTVDSLI
Sbjct: 481 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 540

Query: 541 QGGEWLGPSGATFIPGL 556
           QG EWLG SG TFIPGL
Sbjct: 541 QGAEWLGSSGVTFIPGL 549

[Close]

BLAST of CSPI06G33720 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 577.8 bits (1488), Expect = 9.4e-165
Identity = 310/558 (55.56%), Postives = 386/558 (69.18%), Query Frame = 0


Query: 3   THQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFL--QNICHK 62
           ++QP  LL  PK+ + K +  +L F A+L S  F T + I  N + ND   L    ICH 
Sbjct: 4   SNQP--LLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHG 63

Query: 63  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGE 122
           A+D  SC +   SEF    +   N +D L  FL+ S+ ++ ST     + R R N  R +
Sbjct: 64  AHDQDSC-QALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDK 123

Query: 123 AALADCIELMEISNGRIMDSVLALK--NRTSGSIENSHTWLSSVLTNHVTCWDEV-ESSL 182
           A  ADC E+M++S  R+M S+  L+  N    S  N HTWLSSVLTN++TC + + + S+
Sbjct: 124 AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSV 183

Query: 183 SRAAAMDLGLEELIMRGRNSLGMLVSIWGL--DIKNLGELEKKGNGYPSWLKKGDRRLLG 242
           +    +   LE+L+ R R +L + VS+     D+K +       N +PSWL   DR+LL 
Sbjct: 184 NSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMI-----ISNRFPSWLTALDRKLLE 243

Query: 243 VLGREME--PNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKK 302
              + ++   N+VVAKDG+G FKTV EAV + P+ S  R VIYVK+G Y+E +++GKKKK
Sbjct: 244 SSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKK 303

Query: 303 NVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVAL 362
           N+M+VGDG D+TIITGSLNV+DGSTTF+SATVAA GDGF+AQDIWFQNTAGP KHQAVAL
Sbjct: 304 NLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVAL 363

Query: 363 RVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRP 422
           RV ADQ+VINRCRIDAYQDTLYTH+ RQFYRDS ITGTVDFIFGN+AVV QNC I  R P
Sbjct: 364 RVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNP 423

Query: 423 MNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQ 482
              Q NM+TAQGR D NQNT  SIQ+C I ASSDL PVK S+KT+LGRPWK YSRTVIMQ
Sbjct: 424 GAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQ 483

Query: 483 SRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFT 542
           S I + I PAGW  W+G+FAL TLYYGEY+NTGPG+D SKRV W G+ VI    EA++FT
Sbjct: 484 SFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFT 543

Query: 543 VDSLIQGGEWLGPSGATF 552
           V  LIQGG WL P+G TF
Sbjct: 544 VAKLIQGGLWLKPTGVTF 553

[Close]

BLAST of CSPI06G33720 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 439.1 bits (1128), Expect = 5.2e-123
Identity = 253/541 (46.77%), Postives = 322/541 (59.52%), Query Frame = 0


Query: 54  LQNICHKAYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDI--R 113
           L++ C     P  CI    +      ++ T++ D +++ +  ++  V       K +  +
Sbjct: 59  LRSSCSSTRYPELCISAVVT---AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKK 118

Query: 114 QRINSPRGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSH-----TWLSSVLTNH 173
           ++  +PR + AL DC+E ++ +   + ++V  L    +      H     T +SS +TN 
Sbjct: 119 RKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQ 178

Query: 174 VTCWD--------------------EVESSLSRAAAMDLGLEELIMRGRNSLGMLVS--- 233
            TC D                     VE   S A AM   + +  +        + S   
Sbjct: 179 ETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNR 238

Query: 234 ---------IWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGN 293
                       +DI   GEL+ +  G+P+WL  GDRRLL   G  ++ +  VA DGSG 
Sbjct: 239 KLKEENQETTVAVDIAGAGELDSE--GWPTWLSAGDRRLL--QGSGVKADATVAADGSGT 298

Query: 294 FKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVV 353
           FKTV  AV + P+ S  R VI++K G Y ENVEV KKKKN+M +GDG   TIITGS NVV
Sbjct: 299 FKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVV 358

Query: 354 DGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTL 413
           DGSTTF SATVAAVG+ F+A+DI FQNTAGP KHQAVALRVG+D S    C + AYQDTL
Sbjct: 359 DGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTL 418

Query: 414 YTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTG 473
           Y HSNRQF+    I GTVDFIFGNAAVVLQ+C I  RRP + Q NMVTAQGR DPNQNTG
Sbjct: 419 YVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTG 478

Query: 474 TSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLIQPAGWAEWNGDFAL 533
             IQ+C I A+SDL+ VK S  TYLGRPWKEYS+TVIMQS I D+I+P GW+EW G FAL
Sbjct: 479 IVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFAL 538

Query: 534 KTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQGGEWLGPSGATFIPG 556
            TL Y EYSNTG G+  + RVKW G+ VIT+ +EAQK+T    I GG WL  +G  F  G
Sbjct: 539 NTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLG 592

[Close]

BLAST of CSPI06G33720 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 434.9 bits (1117), Expect = 9.9e-122
Identity = 250/567 (44.09%), Postives = 330/567 (58.20%), Query Frame = 0


Query: 12  PPKTSRSKTIPTLLCFA----AVLCSAIF-----ITNKFIKPNPSENDLPFLQNICHKAY 71
           P    ++KT   L   A     V+C  IF     + +   +P  +      +   C K+ 
Sbjct: 24  PELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSL 83

Query: 72  DPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSPRGEAA 131
            P+ CI+    +FP S+  T +E + +      ++ K       +  I      PR  +A
Sbjct: 84  YPNLCID-TLLDFPGSL--TADENELIHISFNATLQKFSKALYTSSTITYTQMPPRVRSA 143

Query: 132 LADCIELMEISNG---RIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTC---WDEVESS 191
              C+EL++ S     R + SV+ +    S S  +  TWLSS +TNH TC   +DE+E  
Sbjct: 144 YDSCLELLDDSVDALTRALSSVVVVSGDESHS--DVMTWLSSAMTNHDTCTDGFDEIEGQ 203

Query: 192 LSRAAAMDLG-LEELIMRGRNSL----GMLVSIWGLDIKNLGEL--EKKGNGYPSWLKKG 251
                   +G +++L     N L    G +  + G+ + N  +L   ++    P+WLK+ 
Sbjct: 204 GGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEELPNWLKRE 263

Query: 252 DRRLLGVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTY-EENVEV 311
           DR LLG     ++ +I V+KDGSG FKT+ EA++  P+ S  R VIYVK G Y EEN++V
Sbjct: 264 DRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKV 323

Query: 312 GKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKH 371
           G+KK N+M +GDG   T+ITG  ++ D  TTF +AT AA G GFI +D+ F+N AGP KH
Sbjct: 324 GRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKH 383

Query: 372 QAVALRVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKI 431
           QAVALRVG D +V+ RC I  YQD LY HSNRQF+R+  I GTVDFIFGNAAV+LQ+C I
Sbjct: 384 QAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNI 443

Query: 432 EPRRPMNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSR 491
             R+PM  Q   +TAQ R DPNQNTG SI  C ++A+ DLE  K S  TYLGRPWK YSR
Sbjct: 444 YARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSR 503

Query: 492 TVIMQSRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSE 551
            V M S +GD I P GW EWNG FAL +LYYGEY N G GS + +RVKW GYHVITS  E
Sbjct: 504 VVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVE 563

Query: 552 AQKFTVDSLIQGGEWLGPSGATFIPGL 556
           A KFTV   I G  WL  +G +F  GL
Sbjct: 564 ASKFTVAQFISGSSWLPSTGVSFFSGL 585

[Close]

BLAST of CSPI06G33720 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 425.2 bits (1092), Expect = 7.8e-119
Identity = 234/500 (46.80%), Postives = 312/500 (62.40%), Query Frame = 0


Query: 58  CHKAYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDIRQRINSP 117
           C +  +P  C            IK  +E +FL+  ++  + + I     A  +  +    
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIK--SESEFLKISMKLVLDRAILAKTHAFTLGPKCRDT 93

Query: 118 RGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSL 177
           R +AA  DCI+L +++  +I ++   +      S  ++ TWLS+ LTN  TC        
Sbjct: 94  REKAAWEDCIKLYDLTVSKINET---MDPNVKCSKLDAQTWLSTALTNLDTC-------- 153

Query: 178 SRAAAMDLGLEELI--MRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLG 237
            RA  ++LG+ +++  +   N   +L +   ++         + +G+PSW+K GDR+LL 
Sbjct: 154 -RAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPFNYTPPEKDGFPSWVKPGDRKLL- 213

Query: 238 VLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNV 297
                 + N VVAKDGSGNFKT+KEA+++       R VIYVK+G Y EN+E+  +KKNV
Sbjct: 214 -QSSTPKDNAVVAKDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNV 273

Query: 298 MIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRV 357
           M+ GDG+  TIITGS +V  G+TTF SATVAAVGDGFIA+ I F+NTAG    QAVALR 
Sbjct: 274 MLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRS 333

Query: 358 GADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMN 417
           G+D SV  +C  +AYQDTLY HSNRQFYRD  + GTVDFIFGNAA VLQNC I  RRP  
Sbjct: 334 GSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-R 393

Query: 418 NQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSR 477
           ++ N +TAQGR DPNQNTG  I    + A+SDL PV  S KTYLGRPW++YSRTV M++ 
Sbjct: 394 SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTS 453

Query: 478 IGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVD 537
           +  LI P GW EW+G+FALKTL+Y E+ NTGPG+  S RV W G+ V+ S SEA KFTV 
Sbjct: 454 LDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVG 511

Query: 538 SLIQGGEWLGPSGATFIPGL 556
           + + GG W+ PS   F  GL
Sbjct: 514 TFLAGGSWI-PSSVPFTSGL 511

[Close]

BLAST of CSPI06G33720 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 423.7 bits (1088), Expect = 2.3e-118
Identity = 246/529 (46.50%), Postives = 312/529 (58.98%), Query Frame = 0


Query: 54  LQNICHKAYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTTERAKDI--R 113
           L+++C     P  C    A+     +   T++ + +++ L  +   V       K +  +
Sbjct: 62  LKSVCSSTLYPELCFSAVAATGGKEL---TSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 121

Query: 114 QRINSPRGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSH-----TWLSSVLTNH 173
           ++  +PR   AL DC+E ++ +   +  +V  L           H     T +SS +TN 
Sbjct: 122 RKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQ 181

Query: 174 VTC-----WDEVESSLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKK--- 233
            TC     +D+ +  + +A     G   +     N+L M+ ++   DI N    +K    
Sbjct: 182 GTCLDGFSYDDADRKVRKALLK--GQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 241

Query: 234 ----------------GNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGNFKTVKEAV 293
                            +G+P WL  GDRRLL   G  ++ +  VA DGSG+F TV  AV
Sbjct: 242 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLL--QGSTIKADATVADDGSGDFTTVAAAV 301

Query: 294 ESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKS 353
            + P+KS  R VI++K G Y ENVEV KKK N+M +GDG   TIITGS NVVDGSTTF S
Sbjct: 302 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 361

Query: 354 ATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSNRQF 413
           ATVAAVG+ F+A+DI FQNTAGP KHQAVALRVG+D S   +C + AYQDTLY HSNRQF
Sbjct: 362 ATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQF 421

Query: 414 YRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTGTSIQQCDI 473
           +    ITGTVDFIFGNAA VLQ+C I  RRP + Q NMVTAQGR DPNQNTG  IQ C I
Sbjct: 422 FVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRI 481

Query: 474 VASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLIQPAGWAEWNGDFALKTLYYGEY 533
             +SDL  VK +  TYLGRPWKEYSRTVIMQS I D+I+P GW EW+G FAL TL Y EY
Sbjct: 482 GGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREY 541

Query: 534 SNTGPGSDVSKRVKWDGYHVITSPSEAQKFTVDSLIQGGEWLGPSGATF 552
            N G G+  + RVKW GY VITS +EAQ FT    I GG WL  +G  F
Sbjct: 542 LNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

The following BLAST results are available for this feature:

BLAST of CSPI06G33720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83947	7.3e-170	55.64	Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=20423...	[more]
Q1JPL7	1.3e-163	55.56	Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PM...	[more]
P09607	1.1e-160	53.85	Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2	[more]
Q96575	4.0e-160	54.12	Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1	[more]
P14280	7.1e-157	53.76	Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5	[more]

BLAST of CSPI06G33720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHV5	0.0e+00	99.46	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1	[more]
A0A5D3CNY5	1.2e-303	94.05	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00664...	[more]
A0A1S3B018	3.4e-303	94.05	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1	[more]
A0A6J1KRW2	1.1e-253	80.25	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1	[more]
A0A6J1CN37	6.7e-243	78.61	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1	[more]

BLAST of CSPI06G33720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134752.1	0.0e+00	99.46	pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cu...	[more]
TYK13062.1	2.4e-303	94.05	pectinesterase [Cucumis melo var. makuwa]	[more]
XP_008439965.1	7.0e-303	94.05	PREDICTED: pectinesterase [Cucumis melo]	[more]
XP_038881670.1	4.8e-288	89.57	pectinesterase [Benincasa hispida]	[more]
XP_023002934.1	2.3e-253	80.25	pectinesterase-like [Cucurbita maxima]	[more]

BLAST of CSPI06G33720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11580.1	9.4e-165	55.56	methylesterase PCR A	[more]
AT3G14310.1	5.2e-123	46.77	pectin methylesterase 3	[more]
AT5G53370.1	9.9e-122	44.09	pectin methylesterase PCR fragment F	[more]
AT2G45220.1	7.8e-119	46.80	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53830.1	2.3e-118	46.50	pectin methylesterase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 49..200 e-value: 2.7E-15 score: 66.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 25..174 e-value: 2.2E-15 score: 54.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 53..179 e-value: 6.5E-16 score: 59.0
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 244..540 e-value: 3.8E-153 score: 508.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 238..555 e-value: 9.1E-126 score: 421.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 52..203 e-value: 1.4E-21 score: 79.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 53..175
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 14..554
None	No IPR available	PANTHER	PTHR31707:SF333	PECTINESTERASE	coord: 14..554
None	No IPR available	CDD	cd15799	PMEI-like_4	coord: 51..204 e-value: 2.70961E-29 score: 110.963
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 388..397
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 242..554

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G33720.1	CSPI06G33720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity