CSPI03G31030 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G31030
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAmmonium transporter
LocationChr3: 28200076 .. 28203265 (+)
RNA-Seq ExpressionCSPI03G31030
SyntenyCSPI03G31030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCGGATCCCCATCATGGCTCAACACCGGCGACAATTCCTGGCAAATTACAGCCTCCACTCTCGTAGGACTTCAGAGCATGCCTGGACTTGTGATTCTCTACGCCAGCATCGTCAAAAAGAAATGGGCTGTTAATTCAGCGTTTATGGCTCTTTACGCCTTCGCCGCCGTTCTAATTTGTTGGGTCGTCGTCGGTTACAGAATGGCCTTCGGCGACCAGTTGATTCCTTTGTGGGGCAAAGGAATTCCAGCTCTGTCCCAAAGTTATCTAATAGGGCAAGGCAATATCCCTGAGAGTGGCGTGAACGATGGAAGAGGAACACCCAGAATTCAACCCAATGTTCCCATGGCTTCACTTGTGTATTTTCAATTTACTTTTGCCGCGATTACTGTGATTTTACTTGGTGGGTCTGTTCTTGCAAGAATGAACATCAAAGCTTGGATGGCCTTTGTTCCTCTTTGGGTAATTTTCTCATACACCGTCGGCGCATACAGTGTTTGGGGCGGTGGGTTTCTGTTTCAGTGGGGAGTAATTGATTACTCCGGTGGGTATGTAATTCATCTTTCGTCTGGAATTGCTGGGTTAACAGCAGCTTATTGGGTAAGAACTCGATTTGGGGTTCTTGATTGGCGAACACGAAGACGCCTAACGGAGACTTTGACAGAGGAGTCGCGACACTGTGCAAAGGGGAAACTCAATCATGACGAGGGTGAATGGAGATGAAACCCACAACAAATGGAGGATTTTGACTCGGGCTCCTTATGAGAAATGATTTGCTAGAGATTTCTTATTGTATTTTGATTACTTTATTTTTTCTAAAACCATGTGTTATTTAAGTTAGTCACTCATTGGACTTTAGCTCACATTTTCAAAATATTTTCTCACTGTTTCAGGTAGTGATCGTGATTTCAGTGTAACAACCCAACTCTTTATATTGAGCTAAGGTAATTACCTTAAATATAAATAACAATTTAAACAATTTCTTAAAAAGAGGAAAACTAAATTTTCATTAAAAAGGTTTTGTTGTTTTTGTGTTTGGGATTATTAGGTTGGGCCGAGGATAAAAAGTGACAGAGAGAGGTTTCCGCCGAATAACGTGTTGCTTATGTTGGCCGGAGCAGGGCTGTTGTGGATGGGGTGGTCGGGTTTTAACGGGGGAGCACCGCACTCGGCGAACGTGGTGGCGTCGATAGCGGTGCTGAATACGAACGTGAGTGCCGCGACGAGCTTGTTGGTTTGGACCATTTTGGATGTGTTCTACTTTGGGAAGCCATCGGTGATTGGAGCCATTCAGGGTATGATGACTGGCTTGGCTTGCGTTACACCTGGCGCTGGTAATTACCATATCAACCTTATTTTAAACCTTTAATTATTCAACTCATATATACATACATTTATAATTATGTTTGATTTATATTTCAAGTATAAAAATCAAGAGTTTCGTCTAATTAATATAATTTTGTGTTAAATAACATTGCCTAAGATGCTCCTGAAAATACATGAGTTTTAAGGTTTCGAATTTAGAGTAAATTTGTTGATTTTATAAACACTTAGATATGTTATGAACTTAGAGATGAAAGATTATAGTTTTAAAAAGGCAGTATAGAATTCAATGTGAGACATGTGAACACAATAAGTTGACATATAATATATAACTTTGATCAAATTATCATTCAAATCCTTCTACTCGAAATCCTTATACTAAAATAAACAAAAAAAAAAAAATACATATAGACGAATTAAAAGAGGGTGTGATATTAGAATGTCATTTTCTTGATATCTACATTTCAATGAGATGTTGATTATAAATATGATTTATGAAACCACAATAATAATTAAGATTTGAAAGAAACAATTGAAAACAGGAGTCGTGCAGACGTGGGCGGCTATCATAATGGGAATCCTAGCAGGAAGCATTCCATGGCTATCCATGATGGTCCTTCATAAAAAATTGAGTTTCTTGCAAAAGGTAATTTCTTCAACCTTCCATCTTAATCCATTTCTATATATTCGCTTCTCAATTTTCAAACAAAGTTTAATTCAATTATCTTTAATTAATTTATTAAAACTTGCTGATAAAGGACAATGGGGTTTCTGGAAACTACTGAGAATAATTGTTTTATTTTTTCCCAAAAGAAAATGACTTCAAAATTAAATATTTCCCAATTGCTGCCAATTTTTTAATCATATTTGTTGTTTGACAAATCATTTTCATAAGGGGGCCCCTCCTAGTCCTTGGGTTGTTGCCTTTGTACTATTTTTGTTGTTTGTGGATTTTTCTTCCAAACTTCACTTTCCGCCATCTTTGAATATTATTCCACCCCGATACGACGTGGTTTCGGATCGGTATGCATTGTAAAAAAAAACCGCTCTTTAAAGCCTTGCCTTCATTTTTCATTTTGCTTTTATTCAGAATCAAATAGGATTTTCCCTAAGTTTGGAAGAACGTTGGAATCTTAGGCAGAAGCGAAATTTTCAATTCATGAAATAAGAAAAAACACATTGATTAAAGGGAAATGCTCTAAAATAGCAAAGTGGGAAAATAATTTTTTAATAAAGAAAATGGTAAATTGTAGGTTTGGAAACCACTATTGTTTTTTGAAAAGTTGGTTCAATTGAAATAGATGTGTGAAGTTGGTAATTAACTAACCCATCACAATTTTTTTTAAGAACCTTCCATTCCCATACTTTACTAGACCATTAATGACTATTAAGAATGATTAAAACTCACACGTGTATACAACAACAGGCTGATGACACGCTTGGCGTATTTCACACGCACGCAGTGGCAGGATTAATGGGCGGGCTTTTAACTGGGCTTTTAGCGGAGCCCACTCTTTGCGATCTCTACCTACCGATCATCGGCACGCGCGGCGCCTTTTACGGCGGAAGCGGCGGCGTTCAGTTCCTTAAACAACTGGTCGGATCCACCTTCATCATAGGCTGGAACATAGTCTCCACCACCGTAATACTCCTCTTTATTCGCCTCTTTATGCCCTTGAGAATGCCCGATGAGGAACTCATGATCGGCGACGACGCCGTCCACGGCGAGGAGGCTTATGCTCTTTGGGGCGACGGCGAGAAATTCGATCCAAGAAGGCACGGCAATGCGGCAGCCAATATGGAAGAAGGAGCCGGATCGCCTTATATAAATGGTGCTAGAGGAGTGATCATCGAATTGTAA

mRNA sequence

ATGGCCGCCGGATCCCCATCATGGCTCAACACCGGCGACAATTCCTGGCAAATTACAGCCTCCACTCTCGTAGGACTTCAGAGCATGCCTGGACTTGTGATTCTCTACGCCAGCATCGTCAAAAAGAAATGGGCTGTTAATTCAGCGTTTATGGCTCTTTACGCCTTCGCCGCCGTTCTAATTTGTTGGGTCGTCGTCGGTTACAGAATGGCCTTCGGCGACCAGTTGATTCCTTTGTGGGGCAAAGGAATTCCAGCTCTGTCCCAAAGTTATCTAATAGGGCAAGGCAATATCCCTGAGAGTGGCGTGAACGATGGAAGAGGAACACCCAGAATTCAACCCAATGTTCCCATGGCTTCACTTGTGTATTTTCAATTTACTTTTGCCGCGATTACTGTGATTTTACTTGGTGGGTCTGTTCTTGCAAGAATGAACATCAAAGCTTGGATGGCCTTTGTTCCTCTTTGGGTAATTTTCTCATACACCGTCGGCGCATACAGTGTTTGGGGCGGTGGGTTTCTGTTTCAGTGGGGAGTAATTGATTACTCCGGTGGGTATGTAATTCATCTTTCGTCTGGAATTGCTGGGTTAACAGCAGCTTATTGGGTTGGGCCGAGGATAAAAAGTGACAGAGAGAGGTTTCCGCCGAATAACGTGTTGCTTATGTTGGCCGGAGCAGGGCTGTTGTGGATGGGGTGGTCGGGTTTTAACGGGGGAGCACCGCACTCGGCGAACGTGGTGGCGTCGATAGCGGTGCTGAATACGAACGTGAGTGCCGCGACGAGCTTGTTGGTTTGGACCATTTTGGATGTGTTCTACTTTGGGAAGCCATCGGTGATTGGAGCCATTCAGGGTATGATGACTGGCTTGGCTTGCGTTACACCTGGCGCTGGAGTCGTGCAGACGTGGGCGGCTATCATAATGGGAATCCTAGCAGGAAGCATTCCATGGCTATCCATGATGGTCCTTCATAAAAAATTGAGTTTCTTGCAAAAGGCTGATGACACGCTTGGCGTATTTCACACGCACGCAGTGGCAGGATTAATGGGCGGGCTTTTAACTGGGCTTTTAGCGGAGCCCACTCTTTGCGATCTCTACCTACCGATCATCGGCACGCGCGGCGCCTTTTACGGCGGAAGCGGCGGCGTTCAGTTCCTTAAACAACTGGTCGGATCCACCTTCATCATAGGCTGGAACATAGTCTCCACCACCGTAATACTCCTCTTTATTCGCCTCTTTATGCCCTTGAGAATGCCCGATGAGGAACTCATGATCGGCGACGACGCCGTCCACGGCGAGGAGGCTTATGCTCTTTGGGGCGACGGCGAGAAATTCGATCCAAGAAGGCACGGCAATGCGGCAGCCAATATGGAAGAAGGAGCCGGATCGCCTTATATAAATGGTGCTAGAGGAGTGATCATCGAATTGTAA

Coding sequence (CDS)

ATGGCCGCCGGATCCCCATCATGGCTCAACACCGGCGACAATTCCTGGCAAATTACAGCCTCCACTCTCGTAGGACTTCAGAGCATGCCTGGACTTGTGATTCTCTACGCCAGCATCGTCAAAAAGAAATGGGCTGTTAATTCAGCGTTTATGGCTCTTTACGCCTTCGCCGCCGTTCTAATTTGTTGGGTCGTCGTCGGTTACAGAATGGCCTTCGGCGACCAGTTGATTCCTTTGTGGGGCAAAGGAATTCCAGCTCTGTCCCAAAGTTATCTAATAGGGCAAGGCAATATCCCTGAGAGTGGCGTGAACGATGGAAGAGGAACACCCAGAATTCAACCCAATGTTCCCATGGCTTCACTTGTGTATTTTCAATTTACTTTTGCCGCGATTACTGTGATTTTACTTGGTGGGTCTGTTCTTGCAAGAATGAACATCAAAGCTTGGATGGCCTTTGTTCCTCTTTGGGTAATTTTCTCATACACCGTCGGCGCATACAGTGTTTGGGGCGGTGGGTTTCTGTTTCAGTGGGGAGTAATTGATTACTCCGGTGGGTATGTAATTCATCTTTCGTCTGGAATTGCTGGGTTAACAGCAGCTTATTGGGTTGGGCCGAGGATAAAAAGTGACAGAGAGAGGTTTCCGCCGAATAACGTGTTGCTTATGTTGGCCGGAGCAGGGCTGTTGTGGATGGGGTGGTCGGGTTTTAACGGGGGAGCACCGCACTCGGCGAACGTGGTGGCGTCGATAGCGGTGCTGAATACGAACGTGAGTGCCGCGACGAGCTTGTTGGTTTGGACCATTTTGGATGTGTTCTACTTTGGGAAGCCATCGGTGATTGGAGCCATTCAGGGTATGATGACTGGCTTGGCTTGCGTTACACCTGGCGCTGGAGTCGTGCAGACGTGGGCGGCTATCATAATGGGAATCCTAGCAGGAAGCATTCCATGGCTATCCATGATGGTCCTTCATAAAAAATTGAGTTTCTTGCAAAAGGCTGATGACACGCTTGGCGTATTTCACACGCACGCAGTGGCAGGATTAATGGGCGGGCTTTTAACTGGGCTTTTAGCGGAGCCCACTCTTTGCGATCTCTACCTACCGATCATCGGCACGCGCGGCGCCTTTTACGGCGGAAGCGGCGGCGTTCAGTTCCTTAAACAACTGGTCGGATCCACCTTCATCATAGGCTGGAACATAGTCTCCACCACCGTAATACTCCTCTTTATTCGCCTCTTTATGCCCTTGAGAATGCCCGATGAGGAACTCATGATCGGCGACGACGCCGTCCACGGCGAGGAGGCTTATGCTCTTTGGGGCGACGGCGAGAAATTCGATCCAAGAAGGCACGGCAATGCGGCAGCCAATATGGAAGAAGGAGCCGGATCGCCTTATATAAATGGTGCTAGAGGAGTGATCATCGAATTGTAA

Protein sequence

MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL*
Homology
BLAST of CSPI03G31030 vs. ExPASy Swiss-Prot
Match: Q9M6N7 (Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2)

HSP 1 Score: 713.4 bits (1840), Expect = 1.7e-204
Identity = 349/471 (74.10%), Postives = 398/471 (84.50%), Query Frame = 0

Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P WLN GDN+WQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL+CWV+
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQ 125
           + Y+MAFG++L+P WGKG PA  Q YL GQ  IP S V          P  PMA+LVYFQ
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNV--------AAPYFPMATLVYFQ 132

Query: 126 FTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDYSGG 185
           FTFAAIT IL+ GSVL RMNIKAWMAFVPLW+IFSYTVGAYS+WGGGFL+QWGVIDYSGG
Sbjct: 133 FTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDYSGG 192

Query: 186 YVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSAN 245
           YVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP++AN
Sbjct: 193 YVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYAAN 252

Query: 246 VVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVVQTWAA 305
           + +SIAVLNTN+SAATSLLVWT LDV +FGKPSVIGAIQGM+TGLA VTPGAG++QTWAA
Sbjct: 253 LTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTWAA 312

Query: 306 IIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEPTLCDL 365
           II+G+++G+ PW SMM++HKK + LQK DDTL VF+THAVAGL+GG++TGL A P LC L
Sbjct: 313 IIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDLCVL 372

Query: 366 YLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPDEELMI 425
            LP+  TRGAFYGG+GG Q LKQL G+ FI  WN+VSTT+ILL IR+F+PLRM +EEL I
Sbjct: 373 VLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEELGI 432

Query: 426 GDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           GDDA HGEEAYALWGDGEKFD  RH       +E A   Y++GARGV I L
Sbjct: 433 GDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 475

BLAST of CSPI03G31030 vs. ExPASy Swiss-Prot
Match: Q84KJ7 (Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-1 PE=2 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.2e-192
Identity = 333/476 (69.96%), Postives = 388/476 (81.51%), Query Frame = 0

Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P WLN GDN+WQ+TASTLVG+QSMPGLV+LY SIVKKKWAVNSAFMALYA+A+ L+ WV+
Sbjct: 15  PDWLNKGDNAWQLTASTLVGIQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASSLLVWVL 74

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQ 125
           VG+RMAFGDQL+P WGK   AL+QSYL+G+  +P +        PR +P  P A+LV FQ
Sbjct: 75  VGFRMAFGDQLLPFWGKAGVALTQSYLVGRATLPATAHG---AIPRTEPFYPEATLVLFQ 134

Query: 126 FTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDYSGG 185
           F FAAIT++LL GSVL RMNIKAWMAF PLW++ SYTVGA+S+WGGGFL++WGVIDYSGG
Sbjct: 135 FEFAAITLVLLAGSVLGRMNIKAWMAFTPLWLLLSYTVGAFSLWGGGFLYRWGVIDYSGG 194

Query: 186 YVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSAN 245
           YVIHLSSGIAG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLWMGW+GFNGGAP++AN
Sbjct: 195 YVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWMGWAGFNGGAPYAAN 254

Query: 246 VVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVVQTWAA 305
           + AS+AVLNTNV AATSLL+WT LDV +F KPSVIGA+QGMMTGL C+TPGAG+VQTWAA
Sbjct: 255 IAASVAVLNTNVCAATSLLMWTCLDVIFFRKPSVIGAVQGMMTGLVCITPGAGLVQTWAA 314

Query: 306 IIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEPTLCDL 365
           ++MGI AGS+PW +MM+LHKK + L K DDTL VFHTHAVAGL+GG+LTGLLA P L  L
Sbjct: 315 VVMGIFAGSVPWFTMMILHKKSALLMKVDDTLAVFHTHAVAGLLGGILTGLLATPELFSL 374

Query: 366 YLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPDEELMI 425
              + G RGAFYGG G  Q  KQL G+ F+I WN+V TT ILL I LF+PLRMPDE+LMI
Sbjct: 375 ESTVPGLRGAFYGG-GIKQIGKQLGGAAFVIAWNLVVTTAILLGIGLFIPLRMPDEQLMI 434

Query: 426 GDDAVHGEEAYALWGDGEKFDPRRH----GNAAANMEEGAGSPY-INGARGVIIEL 477
           GDDA HGEEAYALWGDGEKFD  RH    G    + +  AG      GARGV I+L
Sbjct: 435 GDDAAHGEEAYALWGDGEKFDATRHDLSRGGGGGDRDGPAGERLSALGARGVTIQL 486

BLAST of CSPI03G31030 vs. ExPASy Swiss-Prot
Match: Q69T29 (Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-3 PE=2 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 2.7e-189
Identity = 315/452 (69.69%), Postives = 378/452 (83.63%), Query Frame = 0

Query: 5   SPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWV 64
           SP WLN GDN+WQ+T++TLVGLQSMPGLVILY SIVKKKWA+NSAFMALYAFAAV ICWV
Sbjct: 16  SPDWLNKGDNAWQMTSATLVGLQSMPGLVILYGSIVKKKWAINSAFMALYAFAAVWICWV 75

Query: 65  VVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGV---NDGRGTPRIQPNVPMASL 124
           V  Y M+FGD+L+P WGK  PAL QS+L+ Q  +  + +   N     P ++P  P+A++
Sbjct: 76  VWAYNMSFGDRLLPFWGKARPALGQSFLVAQSELTATAIRYHNGSAEAPMLKPLYPVATM 135

Query: 125 VYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVID 184
           VYFQ  FA+IT+I+L GS+L RMNIKAWMAFVPLW+ FSYTV A+S+WGGGFLFQWGVID
Sbjct: 136 VYFQCMFASITIIILAGSLLGRMNIKAWMAFVPLWITFSYTVCAFSLWGGGFLFQWGVID 195

Query: 185 YSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP 244
           YSGGYVIHLSSGIAGLTAAYWVGPR  SDRERFPPNN+LL+LAGAGLLW+GW+GFNGG P
Sbjct: 196 YSGGYVIHLSSGIAGLTAAYWVGPRSASDRERFPPNNILLVLAGAGLLWLGWTGFNGGDP 255

Query: 245 HSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVVQ 304
           +SAN+ +S+AVLNT++ A+TSLLVWTILDVF+FGKPSVIGA+QGM+TGL C+TPGAG+VQ
Sbjct: 256 YSANIDSSMAVLNTHICASTSLLVWTILDVFFFGKPSVIGAVQGMITGLVCITPGAGLVQ 315

Query: 305 TWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEPT 364
            WAAI+MGIL+GSIPW +MMVLHKK SF+Q+ DDTLGVFHTHAVAG +GG  TGL AEP 
Sbjct: 316 GWAAIVMGILSGSIPWYTMMVLHKKWSFMQRIDDTLGVFHTHAVAGFLGGATTGLFAEPI 375

Query: 365 LCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPDE 424
           LC L+L I  ++GAFYGG GG QF KQ+ G+ F+  WNIV T++I + I L +PLR+ D+
Sbjct: 376 LCSLFLSIPDSKGAFYGGPGGSQFGKQIAGALFVTAWNIVITSIICVIISLILPLRIADQ 435

Query: 425 ELMIGDDAVHGEEAYALWGDGEKFDPRRHGNA 454
           EL+IGDDAVHGEEAYA+W +GE  D   H  +
Sbjct: 436 ELLIGDDAVHGEEAYAIWAEGELNDMTHHNES 467

BLAST of CSPI03G31030 vs. ExPASy Swiss-Prot
Match: Q84KJ6 (Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-1 PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.5e-187
Identity = 313/455 (68.79%), Postives = 375/455 (82.42%), Query Frame = 0

Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P WLN GDN+WQ+ ++TLVG+QS+PGLVILY SIVKKKWAVNSAFMALYAFAAV +CWV 
Sbjct: 20  PEWLNKGDNAWQMISATLVGMQSVPGLVILYGSIVKKKWAVNSAFMALYAFAAVWLCWVT 79

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPES--------GVNDGRGTPRIQPNVP 125
            GY M+FG +L+P WGK  PAL QS+L+ Q  +P++        G +    TP + P  P
Sbjct: 80  WGYNMSFGHKLLPFWGKARPALGQSFLLAQAVLPQTTQFYKGGGGADAVVETPWVNPLYP 139

Query: 126 MASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQW 185
           MA++VYFQ  FAAIT+ILL GS+L RMNIKAWM FVPLW+ FSYTVGA+S+WGGGFLF W
Sbjct: 140 MATMVYFQCVFAAITLILLAGSLLGRMNIKAWMLFVPLWLTFSYTVGAFSLWGGGFLFHW 199

Query: 186 GVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFN 245
           GV+DYSGGYVIHLSSG+AG TAAYWVGPR   DRERFPPNNVLLML GAG+LWMGW+GFN
Sbjct: 200 GVMDYSGGYVIHLSSGVAGFTAAYWVGPRSTKDRERFPPNNVLLMLTGAGILWMGWAGFN 259

Query: 246 GGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGA 305
           GG P+SAN+ +S+AVLNTN+ AATSLLVWT LDV +F KPSVIGA+QGM+TGL C+TPGA
Sbjct: 260 GGDPYSANIDSSLAVLNTNICAATSLLVWTCLDVIFFKKPSVIGAVQGMITGLVCITPGA 319

Query: 306 GVVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLL 365
           G+VQ WAAI+MGIL+GSIPW +MMV+HK+   LQ+ DDTLGVFHTHAVAG +GG  TGL 
Sbjct: 320 GLVQGWAAIVMGILSGSIPWFTMMVVHKRSRLLQQVDDTLGVFHTHAVAGFLGGATTGLF 379

Query: 366 AEPTLCDLYLPIIGTRGAFY-GGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPL 425
           AEP LC L+LP+  +RGAFY G  GG+QF++Q+ G+ FII WN+V T+++ L +R  +PL
Sbjct: 380 AEPVLCSLFLPVTNSRGAFYPGRGGGLQFVRQVAGALFIICWNVVVTSLVCLAVRAVVPL 439

Query: 426 RMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHG 452
           RMP+EEL IGDDAVHGEEAYALWGDGEK+D  +HG
Sbjct: 440 RMPEEELAIGDDAVHGEEAYALWGDGEKYDSTKHG 474

BLAST of CSPI03G31030 vs. ExPASy Swiss-Prot
Match: Q8S233 (Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-3 PE=2 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 3.3e-187
Identity = 323/481 (67.15%), Postives = 385/481 (80.04%), Query Frame = 0

Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P WLNTGDN WQ+ A+T VGLQSMPGLV+LY SIVKKKWAVNSAFMALYA+A+ LI WV+
Sbjct: 18  PEWLNTGDNGWQLAAATFVGLQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASTLIVWVL 77

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPES---GVNDGRGTPRIQPNVPMASLV 125
           VG+RMAFGD+L+P WGK   AL++ +L+ + ++P +   G +    +PR +P  P AS+V
Sbjct: 78  VGFRMAFGDRLLPFWGKAGAALTEGFLVARASVPATAHYGKDGALESPRTEPFYPEASMV 137

Query: 126 YFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDY 185
            FQF  AAIT++LL GS+L RMNIKAWMAF PLW++FSYTV A+S+WGGGFL+QWGVIDY
Sbjct: 138 LFQFELAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVCAFSLWGGGFLYQWGVIDY 197

Query: 186 SGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 245
           SGGYVIHLSSGIAG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 198 SGGYVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 257

Query: 246 SANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVVQT 305
           + N+ ASIAVLNTNVSAA SLL WT LDV +FGKPSVIGA+QGMMTGL C+TPGAG+V T
Sbjct: 258 APNITASIAVLNTNVSAAASLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHT 317

Query: 306 WAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEPTL 365
           WAAI+MGI  GS+PW SMM+LHK+ + LQK DDTL VFHTHAVAGL+GG LTGL A P L
Sbjct: 318 WAAILMGICGGSLPWFSMMILHKRSALLQKVDDTLAVFHTHAVAGLLGGFLTGLFALPDL 377

Query: 366 CDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPDEE 425
             ++  I G RGAFYGG G  Q  KQ+ G+ F++ WN+V+TTVILL + L +PLRMPDE+
Sbjct: 378 TAVHTHIPGARGAFYGG-GIAQVGKQIAGALFVVVWNVVATTVILLGVGLVVPLRMPDEQ 437

Query: 426 LMIGDDAVHGEEAYALWGDGEKFDPRRHGNA------AANMEEGAGSPYIN-GARGVIIE 477
           L IGDDA HGEEAYALWGDGE+FD  RH  A      AA ++E         GARGV I+
Sbjct: 438 LKIGDDAAHGEEAYALWGDGERFDVTRHEGARGGAWGAAVVDEAMDHRLAGMGARGVTIQ 497

BLAST of CSPI03G31030 vs. ExPASy TrEMBL
Match: A0A0A0LCQ1 (Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.1e-273
Identity = 475/476 (99.79%), Postives = 475/476 (99.79%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS
Sbjct: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300

Query: 301 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360
           QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP
Sbjct: 301 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360

Query: 361 TLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 420
           TLCDLYLPIIGTRGAFYGGSGGVQFLKQL GSTFIIGWNIVSTTVILLFIRLFMPLRMPD
Sbjct: 361 TLCDLYLPIIGTRGAFYGGSGGVQFLKQLAGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 420

Query: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL
Sbjct: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 476

BLAST of CSPI03G31030 vs. ExPASy TrEMBL
Match: A0A5A7TLX4 (Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00540 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 5.7e-267
Identity = 462/476 (97.06%), Postives = 467/476 (98.11%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAA SPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAASPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWV+VGYRMAFGDQLIP WGKGIPALSQ+YLI QGNIPESG NDG GTPRIQPNVPMAS
Sbjct: 61  ICWVIVGYRMAFGDQLIPFWGKGIPALSQNYLIAQGNIPESGANDGNGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300

Query: 301 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360
           QTWAAIIMGILAGSIPWLSMMVLHK+LSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP
Sbjct: 301 QTWAAIIMGILAGSIPWLSMMVLHKRLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360

Query: 361 TLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 420
           TLCDLYLPI GTRGAFYG +GGVQFLKQL GSTF+IGWNIVSTTVILLFIRLFMPLRMPD
Sbjct: 361 TLCDLYLPITGTRGAFYGRNGGVQFLKQLAGSTFVIGWNIVSTTVILLFIRLFMPLRMPD 420

Query: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEG GSPYINGARGVIIEL
Sbjct: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGTGSPYINGARGVIIEL 476

BLAST of CSPI03G31030 vs. ExPASy TrEMBL
Match: A0A1S3B5K4 (Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103486266 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 5.7e-267
Identity = 462/476 (97.06%), Postives = 467/476 (98.11%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAA SPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAASPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWV+VGYRMAFGDQLIP WGKGIPALSQ+YLI QGNIPESG NDG GTPRIQPNVPMAS
Sbjct: 61  ICWVIVGYRMAFGDQLIPFWGKGIPALSQNYLIAQGNIPESGANDGNGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300

Query: 301 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360
           QTWAAIIMGILAGSIPWLSMMVLHK+LSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP
Sbjct: 301 QTWAAIIMGILAGSIPWLSMMVLHKRLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360

Query: 361 TLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 420
           TLCDLYLPI GTRGAFYG +GGVQFLKQL GSTF+IGWNIVSTTVILLFIRLFMPLRMPD
Sbjct: 361 TLCDLYLPITGTRGAFYGRNGGVQFLKQLAGSTFVIGWNIVSTTVILLFIRLFMPLRMPD 420

Query: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEG GSPYINGARGVIIEL
Sbjct: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGTGSPYINGARGVIIEL 476

BLAST of CSPI03G31030 vs. ExPASy TrEMBL
Match: A0A6J1F297 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.2e-254
Identity = 441/478 (92.26%), Postives = 457/478 (95.61%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 10  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESG--VNDGRGTPRIQPNVPM 120
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + NIPESG   NDGR T RI+PN+PM
Sbjct: 70  ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPM 129

Query: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 130 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 189

Query: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 190 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 249

Query: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 300
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 250 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 309

Query: 301 VVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLA 360
           +VQTWAA+IMGIL GSIPW+SMMVLHKKLSFLQKADDTLGVFHTHAVAG+MGGLLTGLLA
Sbjct: 310 IVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLA 369

Query: 361 EPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRM 420
           EPTLCDLYLPI GTRGAFYG +GGVQFLKQL GS FIIGWN+VSTT+ILLFIRLFMPLRM
Sbjct: 370 EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRM 429

Query: 421 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEG  SPYI+GARGVIIEL
Sbjct: 430 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGARGVIIEL 487

BLAST of CSPI03G31030 vs. ExPASy TrEMBL
Match: A0A6J1IZU0 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.6e-253
Identity = 442/478 (92.47%), Postives = 456/478 (95.40%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 10  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESG--VNDGRGTPRIQPNVPM 120
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + +IPESG   NDGR T RI+PN+PM
Sbjct: 70  ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRADIPESGNTNNDGRVTLRIEPNIPM 129

Query: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 130 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 189

Query: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 190 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 249

Query: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 300
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 250 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 309

Query: 301 VVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLA 360
           VVQTWAA+IMGIL GSIPW+SMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLA
Sbjct: 310 VVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLA 369

Query: 361 EPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRM 420
           EPTLCDLYLPI GTRGAFYG +GGVQFLKQL GS FIIGWNIVSTT+ILLFIRLFMPLRM
Sbjct: 370 EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNIVSTTIILLFIRLFMPLRM 429

Query: 421 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           PDE LMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEG  SPYI+GARGVIIEL
Sbjct: 430 PDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGARGVIIEL 487

BLAST of CSPI03G31030 vs. NCBI nr
Match: XP_004137698.1 (ammonium transporter 2 [Cucumis sativus] >KGN58729.1 hypothetical protein Csa_000983 [Cucumis sativus])

HSP 1 Score: 949.9 bits (2454), Expect = 8.4e-273
Identity = 475/476 (99.79%), Postives = 475/476 (99.79%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS
Sbjct: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300

Query: 301 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360
           QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP
Sbjct: 301 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360

Query: 361 TLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 420
           TLCDLYLPIIGTRGAFYGGSGGVQFLKQL GSTFIIGWNIVSTTVILLFIRLFMPLRMPD
Sbjct: 361 TLCDLYLPIIGTRGAFYGGSGGVQFLKQLAGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 420

Query: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL
Sbjct: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 476

BLAST of CSPI03G31030 vs. NCBI nr
Match: XP_008442385.1 (PREDICTED: ammonium transporter 2 [Cucumis melo] >KAA0044194.1 ammonium transporter 2 [Cucumis melo var. makuwa] >TYK24938.1 ammonium transporter 2 [Cucumis melo var. makuwa])

HSP 1 Score: 929.5 bits (2401), Expect = 1.2e-266
Identity = 462/476 (97.06%), Postives = 467/476 (98.11%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAA SPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAASPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWV+VGYRMAFGDQLIP WGKGIPALSQ+YLI QGNIPESG NDG GTPRIQPNVPMAS
Sbjct: 61  ICWVIVGYRMAFGDQLIPFWGKGIPALSQNYLIAQGNIPESGANDGNGTPRIQPNVPMAS 120

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI
Sbjct: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV
Sbjct: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300

Query: 301 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360
           QTWAAIIMGILAGSIPWLSMMVLHK+LSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP
Sbjct: 301 QTWAAIIMGILAGSIPWLSMMVLHKRLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360

Query: 361 TLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 420
           TLCDLYLPI GTRGAFYG +GGVQFLKQL GSTF+IGWNIVSTTVILLFIRLFMPLRMPD
Sbjct: 361 TLCDLYLPITGTRGAFYGRNGGVQFLKQLAGSTFVIGWNIVSTTVILLFIRLFMPLRMPD 420

Query: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEG GSPYINGARGVIIEL
Sbjct: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGTGSPYINGARGVIIEL 476

BLAST of CSPI03G31030 vs. NCBI nr
Match: XP_038904152.1 (ammonium transporter 2 [Benincasa hispida])

HSP 1 Score: 924.5 bits (2388), Expect = 3.8e-265
Identity = 460/476 (96.64%), Postives = 466/476 (97.90%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAA SP WLNTGDNSWQITASTLVGLQSMPGLVILY SIVKKKWAVNSAFMALYAFAAVL
Sbjct: 10  MAAASPPWLNTGDNSWQITASTLVGLQSMPGLVILYGSIVKKKWAVNSAFMALYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMAS 120
           ICWV+VGYRMAFGDQLIPLWGKGIPALSQ YLI Q NIPESG+NDG+GTPRIQPNVPMAS
Sbjct: 70  ICWVIVGYRMAFGDQLIPLWGKGIPALSQDYLINQANIPESGLNDGQGTPRIQPNVPMAS 129

Query: 121 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVI 180
           LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLF+WGVI
Sbjct: 130 LVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFKWGVI 189

Query: 181 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 240
           DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA
Sbjct: 190 DYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGA 249

Query: 241 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 300
           PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV
Sbjct: 250 PHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVV 309

Query: 301 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 360
           QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP
Sbjct: 310 QTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEP 369

Query: 361 TLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 420
           TLCDLY+PIIGTRGAFYG +GGVQFLKQL GSTFIIGWNIVSTTVILLFIRLFMPLRMPD
Sbjct: 370 TLCDLYIPIIGTRGAFYGKNGGVQFLKQLTGSTFIIGWNIVSTTVILLFIRLFMPLRMPD 429

Query: 421 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           EELMIGDDAVHGEEAYALWGDGEKFDPRRHG AAANMEEG GSPYINGARGVIIEL
Sbjct: 430 EELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAAANMEEGTGSPYINGARGVIIEL 485

BLAST of CSPI03G31030 vs. NCBI nr
Match: XP_022934329.1 (ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 887.1 bits (2291), Expect = 6.7e-254
Identity = 441/478 (92.26%), Postives = 457/478 (95.61%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 10  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 69

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESG--VNDGRGTPRIQPNVPM 120
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + NIPESG   NDGR T RI+PN+PM
Sbjct: 70  ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPM 129

Query: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 130 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 189

Query: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 190 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 249

Query: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 300
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 250 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 309

Query: 301 VVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLA 360
           +VQTWAA+IMGIL GSIPW+SMMVLHKKLSFLQKADDTLGVFHTHAVAG+MGGLLTGLLA
Sbjct: 310 IVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLA 369

Query: 361 EPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRM 420
           EPTLCDLYLPI GTRGAFYG +GGVQFLKQL GS FIIGWN+VSTT+ILLFIRLFMPLRM
Sbjct: 370 EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRM 429

Query: 421 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEG  SPYI+GARGVIIEL
Sbjct: 430 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGARGVIIEL 487

BLAST of CSPI03G31030 vs. NCBI nr
Match: KAG7017209.1 (Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 887.1 bits (2291), Expect = 6.7e-254
Identity = 441/478 (92.26%), Postives = 457/478 (95.61%), Query Frame = 0

Query: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 94  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 153

Query: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESG--VNDGRGTPRIQPNVPM 120
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + NIPESG   NDGR T RI+PN+PM
Sbjct: 154 ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPM 213

Query: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 214 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 273

Query: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 274 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 333

Query: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 300
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 334 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 393

Query: 301 VVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLA 360
           +VQTWAA+IMGIL GSIPW+SMMVLHKKLSFLQKADDTLGVFHTHAVAG+MGGLLTGLLA
Sbjct: 394 IVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLA 453

Query: 361 EPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRM 420
           EPTLCDLYLPI GTRGAFYG +GGVQFLKQL GS FIIGWN+VSTT+ILLFIRLFMPLRM
Sbjct: 454 EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRM 513

Query: 421 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEG  SPYI+GARGVIIEL
Sbjct: 514 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGARGVIIEL 571

BLAST of CSPI03G31030 vs. TAIR 10
Match: AT2G38290.1 (ammonium transporter 2 )

HSP 1 Score: 713.4 bits (1840), Expect = 1.2e-205
Identity = 349/471 (74.10%), Postives = 398/471 (84.50%), Query Frame = 0

Query: 6   PSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVV 65
           P WLN GDN+WQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL+CWV+
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 66  VGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQ 125
           + Y+MAFG++L+P WGKG PA  Q YL GQ  IP S V          P  PMA+LVYFQ
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNV--------AAPYFPMATLVYFQ 132

Query: 126 FTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWGVIDYSGG 185
           FTFAAIT IL+ GSVL RMNIKAWMAFVPLW+IFSYTVGAYS+WGGGFL+QWGVIDYSGG
Sbjct: 133 FTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDYSGG 192

Query: 186 YVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSAN 245
           YVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP++AN
Sbjct: 193 YVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYAAN 252

Query: 246 VVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAGVVQTWAA 305
           + +SIAVLNTN+SAATSLLVWT LDV +FGKPSVIGAIQGM+TGLA VTPGAG++QTWAA
Sbjct: 253 LTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTWAA 312

Query: 306 IIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLAEPTLCDL 365
           II+G+++G+ PW SMM++HKK + LQK DDTL VF+THAVAGL+GG++TGL A P LC L
Sbjct: 313 IIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDLCVL 372

Query: 366 YLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLRMPDEELMI 425
            LP+  TRGAFYGG+GG Q LKQL G+ FI  WN+VSTT+ILL IR+F+PLRM +EEL I
Sbjct: 373 VLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEELGI 432

Query: 426 GDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           GDDA HGEEAYALWGDGEKFD  RH       +E A   Y++GARGV I L
Sbjct: 433 GDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 475

BLAST of CSPI03G31030 vs. TAIR 10
Match: AT2G38290.2 (ammonium transporter 2 )

HSP 1 Score: 561.2 bits (1445), Expect = 7.8e-160
Identity = 272/359 (75.77%), Postives = 311/359 (86.63%), Query Frame = 0

Query: 118 MASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQW 177
           MA+LVYFQFTFAAIT IL+ GSVL RMNIKAWMAFVPLW+IFSYTVGAYS+WGGGFL+QW
Sbjct: 1   MATLVYFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQW 60

Query: 178 GVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFN 237
           GVIDYSGGYVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFN
Sbjct: 61  GVIDYSGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFN 120

Query: 238 GGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGA 297
           GGAP++AN+ +SIAVLNTN+SAATSLLVWT LDV +FGKPSVIGAIQGM+TGLA VTPGA
Sbjct: 121 GGAPYAANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGA 180

Query: 298 GVVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLL 357
           G++QTWAAII+G+++G+ PW SMM++HKK + LQK DDTL VF+THAVAGL+GG++TGL 
Sbjct: 181 GLIQTWAAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLF 240

Query: 358 AEPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVSTTVILLFIRLFMPLR 417
           A P LC L LP+  TRGAFYGG+GG Q LKQL G+ FI  WN+VSTT+ILL IR+F+PLR
Sbjct: 241 AHPDLCVLVLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLR 300

Query: 418 MPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 477
           M +EEL IGDDA HGEEAYALWGDGEKFD  RH       +E A   Y++GARGV I L
Sbjct: 301 MAEEELGIGDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 359

BLAST of CSPI03G31030 vs. TAIR 10
Match: AT1G64780.1 (ammonium transporter 1;2 )

HSP 1 Score: 112.1 bits (279), Expect = 1.3e-24
Identity = 130/477 (27.25%), Postives = 193/477 (40.46%), Query Frame = 0

Query: 13  DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVVVGYRMAF 72
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  I + + G+  AF
Sbjct: 52  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGAISYYLFGFAFAF 111

Query: 73  GDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQFTFAAIT 132
           G         G      S+     + PE   +D              S   +Q+ FA   
Sbjct: 112 GTP-----SNGFIGRHHSF-FALSSYPERPGSD-------------FSFFLYQWAFAIAA 171

Query: 133 VILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWG-----------GGFLFQWGVID 192
             +  GS+  R    A++ +      F Y   ++  W               LF  G ID
Sbjct: 172 AGITSGSIAERTQFVAYLIYSTFLTGFVYPTVSHWFWSSDGWASASRSDNNLLFGSGAID 231

Query: 193 YSGGYVIHLSSGIAGLTAAYWVGPRI-KSDRE----RFPPNNVLLMLAGAGLLWMGWSGF 252
           ++G  V+H+  GIAGL  A   GPRI + DR         ++  L++ G  LLW GW GF
Sbjct: 232 FAGSGVVHMVGGIAGLCGALVEGPRIGRFDRSGRSVALRGHSASLVVLGTFLLWFGWYGF 291

Query: 253 NGGA------------PHSA--NVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGA 312
           N G+            P+    + V   AV  T +S  T+ L          G  +VI  
Sbjct: 292 NPGSFLTILKGYDKSRPYYGQWSAVGRTAV-TTTLSGCTAALTTLFSKRLLAGHWNVIDV 351

Query: 313 IQGMMTGLACVTPGAGVVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHT 372
             G++ G A +T G  VV+ WAAI+ G +A  +  +   +L KKL    K DD L     
Sbjct: 352 CNGLLGGFAAITSGCAVVEPWAAIVCGFVASWV-LIGFNLLAKKL----KYDDPLEAAQL 411

Query: 373 HAVAGLMGGLLTGLLAEPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVS 432
           H   G  G + TGL A     +         G F GG GG     Q+V    I+GW  V+
Sbjct: 412 HGGCGAWGLIFTGLFARKEYVNEIYSGDRPYGLFMGG-GGKLLAAQIVQIIVIVGWVTVT 471

Query: 433 TTVILLFIRLFMPLRMPDEELMIGDDAV-HGEEAYALWGDGEKFDPRRHGNAAANME 459
              +   +     LR+  E+ M G D   HG  AYA + D +    +  G+ A  +E
Sbjct: 472 MGPLFYGLHKMNLLRISAEDEMAGMDMTRHGGFAYA-YNDEDDVSTKPWGHFAGRVE 500

BLAST of CSPI03G31030 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 112.1 bits (279), Expect = 1.3e-24
Identity = 129/482 (26.76%), Postives = 192/482 (39.83%), Query Frame = 0

Query: 13  DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVVVGYRMAF 72
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  + + + GY  AF
Sbjct: 42  DNTYLLFSAYLVFSMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 101

Query: 73  GDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQFTFAAIT 132
           G       GK        +  G  +IP +  +               S   +Q+ FA   
Sbjct: 102 GSPSNGFIGK--------HYFGLKDIPTASAD--------------YSNFLYQWAFAIAA 161

Query: 133 VILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWG-----------GGFLFQWGVID 192
             +  GS+  R    A++ +      F Y V ++  W            G  LF  G ID
Sbjct: 162 AGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLFSTGAID 221

Query: 193 YSGGYVIHLSSGIAGLTAAYWVGPRI-KSDRE----RFPPNNVLLMLAGAGLLWMGWSGF 252
           ++G  V+H+  GIAGL  A   GPR+ + D          ++  L++ G  LLW GW GF
Sbjct: 222 FAGSGVVHMVGGIAGLWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLWFGWYGF 281

Query: 253 NGGAPHSANVVASIAVLNTNVSA------ATSLLVWTILDVFYFGKP------SVIGAIQ 312
           N G+ +   V       N   SA       T+L   T      FGK       +V     
Sbjct: 282 NPGSFNKILVTYETGTYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWNVTDVCN 341

Query: 313 GMMTGLACVTPGAGVVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHA 372
           G++ G A +T G  VV+ WAAII G +A  +     ++   KL+   K DD L     H 
Sbjct: 342 GLLGGFAAITGGCSVVEPWAAIICGFVAALV-----LLGCNKLAEKLKYDDPLEAAQLHG 401

Query: 373 VAGLMGGLLTGLLA-EPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWNIVST 432
             G  G + T L A E  L  +Y    G     + G GG     QL+    I GW   + 
Sbjct: 402 GCGAWGLIFTALFAQEKYLNQIYGNKPGRPHGLFMGGGGKLLGAQLIQIIVITGWVSATM 461

Query: 433 TVILLFIRLFMPLRMPDEELMIGDDAV-HGEEAYALWGDGE--------KFDPRRHGNAA 457
             +   ++    LR+  E+ M G D   HG  AY  + D E        + +PR    + 
Sbjct: 462 GTLFFILKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDESHKAIQLRRVEPRSPSPSG 495

BLAST of CSPI03G31030 vs. TAIR 10
Match: AT3G24290.1 (ammonium transporter 1;5 )

HSP 1 Score: 110.5 bits (275), Expect = 3.7e-24
Identity = 124/458 (27.07%), Postives = 188/458 (41.05%), Query Frame = 0

Query: 13  DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVVVGYRMAF 72
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  + + + GY  AF
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 73  GDQLIPLWGKGIPALSQSYLIGQGNIPESGVNDGRGTPRIQPNVPMASLVYFQFTFAAIT 132
           G+             S    IG+ N          G           S   +Q+ FA   
Sbjct: 106 GE-------------SSDGFIGRHNF---------GLQNFPTLTSDYSFFLYQWAFAIAA 165

Query: 133 VILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWG-GGF---------LFQWGVIDY 192
             +  GS+  R    A++ +      F Y V ++  W   G+         LF  G ID+
Sbjct: 166 AGITSGSIAERTKFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSEDRLFGTGAIDF 225

Query: 193 SGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPP---------NNVLLMLAGAGLLWMGW 252
           +G  V+H+  GIAGL  A   GPRI     RFP          ++  L++ G  LLW GW
Sbjct: 226 AGSGVVHMVGGIAGLWGALIEGPRI----GRFPDGGHAIALRGHSASLVVLGTFLLWFGW 285

Query: 253 SGFNGGA------PHSA-------NVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVI 312
            GFN G+      P+++       + +   AV  T +S  T+ L          G  +V 
Sbjct: 286 YGFNPGSFTKILIPYNSGSNYGQWSGIGRTAV-TTTLSGCTAALTTLFGKRLLSGHWNVT 345

Query: 313 GAIQGMMTGLACVTPGAGVVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVF 372
               G++ G A +T G  VV  WAAI+ G +A  +     ++   KL+ L K DD L   
Sbjct: 346 DVCNGLLGGFAAITAGCSVVDPWAAIVCGFVASLV-----LIGCNKLAELLKYDDPLEAA 405

Query: 373 HTHAVAGLMGGLLTGLLA-EPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLVGSTFIIGWN 432
             H   G  G +  GL A E  + ++Y    G     + G GG     QLV    I+GW 
Sbjct: 406 QLHGGCGAWGLIFVGLFAKEKYINEVYGASPGRHYGLFMGGGGKLLGAQLVQIIVIVGWV 465

Query: 433 IVSTTVILLFIRLFMPLRMPDEELMIGDD-AVHGEEAY 437
             +   +   ++    LR+ ++  M G D A HG  AY
Sbjct: 466 SATMGTLFFILKKLNLLRISEQHEMRGMDLAGHGGFAY 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M6N71.7e-20474.10Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2[more]
Q84KJ71.2e-19269.96Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2... [more]
Q69T292.7e-18969.69Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
Q84KJ61.5e-18768.79Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
Q8S2333.3e-18767.15Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2... [more]
Match NameE-valueIdentityDescription
A0A0A0LCQ14.1e-27399.79Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1[more]
A0A5A7TLX45.7e-26797.06Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352... [more]
A0A1S3B5K45.7e-26797.06Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103486266 PE=3 SV=1[more]
A0A6J1F2973.2e-25492.26Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1[more]
A0A6J1IZU01.6e-25392.47Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004137698.18.4e-27399.79ammonium transporter 2 [Cucumis sativus] >KGN58729.1 hypothetical protein Csa_00... [more]
XP_008442385.11.2e-26697.06PREDICTED: ammonium transporter 2 [Cucumis melo] >KAA0044194.1 ammonium transpor... [more]
XP_038904152.13.8e-26596.64ammonium transporter 2 [Benincasa hispida][more]
XP_022934329.16.7e-25492.26ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transpor... [more]
KAG7017209.16.7e-25492.26Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT2G38290.11.2e-20574.10ammonium transporter 2 [more]
AT2G38290.27.8e-16075.77ammonium transporter 2 [more]
AT1G64780.11.3e-2427.25ammonium transporter 1;2 [more]
AT4G13510.11.3e-2426.76ammonium transporter 1;1 [more]
AT3G24290.13.7e-2427.07ammonium transporter 1;5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002229Blood group Rhesus C/E/D polypeptidePRINTSPR00342RHESUSRHDcoord: 125..141
score: 31.7
coord: 306..324
score: 25.44
coord: 344..360
score: 23.86
coord: 220..238
score: 41.52
coord: 251..273
score: 24.88
IPR001905Ammonium transporterTIGRFAMTIGR00836TIGR00836coord: 13..436
e-value: 1.1E-113
score: 378.1
IPR029020Ammonium/urea transporterGENE3D1.10.3430.10Ammonium transporter AmtB like domainscoord: 1..439
e-value: 1.5E-117
score: 395.0
IPR024041Ammonium transporter AmtB-like domainPFAMPF00909Ammonium_transpcoord: 16..436
e-value: 5.4E-80
score: 269.0
NoneNo IPR availablePANTHERPTHR43029AMMONIUM TRANSPORTER MEP2coord: 4..473
NoneNo IPR availablePANTHERPTHR43029:SF34AMMONIUM TRANSPORTER 2coord: 4..473
NoneNo IPR availableSUPERFAMILY111352Ammonium transportercoord: 8..418
IPR018047Ammonium transporter, conserved sitePROSITEPS01219AMMONIUM_TRANSPcoord: 181..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G31030.1CSPI03G31030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072488 ammonium transmembrane transport
biological_process GO:0019740 nitrogen utilization
biological_process GO:0015696 ammonium transport
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015398 high-affinity secondary active ammonium transmembrane transporter activity
molecular_function GO:0008519 ammonium transmembrane transporter activity