Cp4.1LG10g04430 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g04430
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCarbonic anhydrase
LocationCp4.1LG10: 1411571 .. 1414779 (-)
RNA-Seq ExpressionCp4.1LG10g04430
SyntenyCp4.1LG10g04430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGCATTGGAAAATGACTCGATCAGCGCTTCCCGCCTTCCATGGAAGCGTTATGAACAGCAACTCTCCAAACTTTTTATTCTTCTAGCTTTTCATTCTCAATTGGATCAGAACCTCCCCAGAAATTTCCCACTGCTCTCTGTCTCTCTCTCTCTCGTTGTTTATACATTATATATTGTTCATAATCTGAGAGAATTGACCGAATTTCGACTCCGATTCCATTGCTTTGTCTTGCAAATCCGTTTCCTCGATTCAGTTTTCGCTCCAGAAGCCATGAAAATTGGGACTACACTCATCGTTTTCTTCTCGCTTCTGCTGTTTTCCCTACATTTGTCTTCAATCTCGGCGCAAGAAGTTGGTAAGCTCTTCACCGTTACTGATATATCTCTGTCTATGTTGTTTTGATTTTCCCTTTTCCTTTTTCTATCTGCGTTTTGCCTTCCATTTAAGCTAATTATGTTTCGGTCTCGATTTTTTTATTTTTGATTCTATCAATTGGTTTTGAGTCTAAGAGGGCTGCTCTCGTCTGAATCGTTTGGCGATTCATTTACTTAATCGTGTAAAAATCGGTGTTAGATCTTGAGATGGAAGCTATAGATTGTTTGGCTCGAATATTTACATTGGTAATTCGAAATAGTTGCAGTAAACTCTTGACTGCAAGTCTGCAACATTGTTTTGATTTCTCCCACCTCCATTTTCATGTTGTTTTCAGATCATATGTTAAGGTTTCTATTTCAATTTCTGATTCTGCTGATTAGTTTTTACGTACCGCTGGTTAGACAAACACGACTCTCCACAATCGTATGATATTGTCCACTTTGAGCATAAGCTCTCGTGGCTTTGTTTTGGGCTTTCCCAAAAGGCCTCACACCAATGGAGTTAGTATTCCTCACTTGTAAACCCGTGATCATTCCCTAAATTAGCCGATATAGGACTTCCATCATCCAACACCGCTCTCATCTGAATCGTTTTATGTGAACAATTTTTCTTTCATTTAATCGTGCAAGTCTGCATCATTGTTTTGATTTCTCCCACTCCATTTTCTTGTTGTGTTTAGACCGTATGTTTAGGTTTCTATGTCCATTTCTGATTCTACTCATTAGTTATTGAATCGACGTACCGCTCTCATCTGAATCGTTTTATGTGAATAACTTCTCATTCCTTTAATCAAAATTAAATGTAAACTCTTGAGATGGAAACTAGAGTTTTGGCTCAAATGCTTACTTCGGTATTTCAAAATCAATGGGTTGCAGAGGATGAGAGGGAATTTGATTACAGAGAAGGGAGTGAGAAAGGGCCAAGACATTGGGGAAAGATAAAGAAAGAGTGGGAAGCTTGTAATAATGGGGTTTTGCAATCTCCCATAGACTTATCCAGCCAGAGAGTGAAGATTGTTCCGAAGTTTGGAGACGTGAAGAGAAGCTACAAACCTTGCAATGCAACGGTCAAAAATAGGGGCCATGACATTTCTGTATGAAATTCAACAGATTCCTTCTCTCTTCATCAAATTTTGTGGTTTCTGATTTCTCTATTTTCTTTCGTTTAATGTGCCTGTGGATGTAGATATATTGGGTTGGCAAGGCAGGATCAATCAAGGTAAACGGCGTTGTTTATGATCTTCAACAAGCGCATTGGCACTCACCTTCAGAGCATTCCATCAATGGAAGAAGGTATCTCTTTTGCATTAAAAAGATGTTACAAACCCATCTTCTTTGACCCTGAAATTAATAGAATATTTTAATTATTGTATCAAGATATAATTATTCTTTTGAATTATCTAATGATGATTCTAAATTGTTGATTAACATTGATAACCCATCAGGTATGACATGGAACTCCACATGGTTCACCAAAGTTCTGATCCCAATGGGAAGAACAAGATTGCTGTTGTTGGTCAACTCTATACAATTGGCAAACCAGACCCTTTTCTCGCACAGGTAAACCACCTCCAAACACAACAAAACAACCCAATTCAAATGGCTCGATTATAAGCTTGAATAGGTCCAAGTGATTTCTGAGTTTTTTTTGTGGTCTAGTTCATGATTTTCCTAAAATTGATTTGTGAGAATTCGAATCGGTACAAATTATTAATCAAAAGGATAACATTTTACCTGTTATGAGTAATAGTTAATTATGCATGTGTCTTTTAATCTGATTTTACTGTATTCTTTTGAGTTCTCTTCAGCTCACAAACGTTTTCTTGAAGCAAAGATTAGTTCAAATCTTAGAGCATTGTATTTAATCTCATCGGTTTACTTGTAAATGAAGTATTCAAACTATCCCAACCAACCAATCCAATCCCCATACTGTTGATTAGATTAAGGTCATTTGATTCAAACAAATTTATAATCCAAATAACACACGGCAACCATTTTATCATTCATTAACAAAAACTTATTGTTTCTTGATGTTCAGTTCAGTAAGCAAATAAGCTACATGATTGATCGAGAGCATGAAATGGGGATTGGAGAGATAGATCCTGCAGATATCAAGTTTGGTGGTAAAAAGTACTACAAATACCTTGGTTCTCTCACTGTTCCTCCCTGTAGTGAAGGTGTAATTTGGGTCATAAACAAGAAGGTGAGGAAGAGTTTTTTTCGAACATCGAAAGTAAATCTTAAAACAGGTTGATAGAAATTCCCTTCTTGACTGTGTCCAAAATGAATGAAATTTCGTAGGCAGAGTTTAACATATATCATCAAATGGATTAGAATAAGAAACAGGTGTCTGATTCTTCGAATCGAAAACTTTAAACTTAGCTCTAGCCATTTCGAGTTTGTTACAATATTGCCTACATGAATAGCTCAATCTTATCAGAGTTTTTGAATGGTAATGCAACAGATAAGAACAGTTTCAAAAGAGCAAGTGCGACTTCTGAGAGAAGCTGTTCATGATGTAAGAAACTCAATTACTTTTACCACTAATTTCTGATAAATTTGTTGCTTCAAATGTTTTTGAGTACATATTTTCTCAATTGCAGTATGCAGAGCGCAATGCAAGGCCATTGCAGCCGCTCAATAACCGGGAAGTCCAAGTCTTATCGACCTCTCAAAGATCTAGAACCTGATCACATGGACACCATCTTCATTGAATGTCAAGGTCCCTGTTTTATAATATTTTGGTGAAAAAAAAATATATAAATTTATCACTATCCTTTTTATAAAAAATATTATTATCCATCATCAATGATTTTGTTCTTCATGATAATC

mRNA sequence

AAGGCATTGGAAAATGACTCGATCAGCGCTTCCCGCCTTCCATGGAAGCGTTATGAACAGCAACTCTCCAAACTTTTTATTCTTCTAGCTTTTCATTCTCAATTGGATCAGAACCTCCCCAGAAATTTCCCACTGCTCTCTGTCTCTCTCTCTCTCGTTGTTTATACATTATATATTGTTCATAATCTGAGAGAATTGACCGAATTTCGACTCCGATTCCATTGCTTTGTCTTGCAAATCCGTTTCCTCGATTCAGTTTTCGCTCCAGAAGCCATGAAAATTGGGACTACACTCATCGTTTTCTTCTCGCTTCTGCTGTTTTCCCTACATTTGTCTTCAATCTCGGCGCAAGAAGTTGAGGATGAGAGGGAATTTGATTACAGAGAAGGGAGTGAGAAAGGGCCAAGACATTGGGGAAAGATAAAGAAAGAGTGGGAAGCTTGTAATAATGGGGTTTTGCAATCTCCCATAGACTTATCCAGCCAGAGAGTGAAGATTGTTCCGAAGTTTGGAGACGTGAAGAGAAGCTACAAACCTTGCAATGCAACGGTCAAAAATAGGGGCCATGACATTTCTATATATTGGGTTGGCAAGGCAGGATCAATCAAGGTAAACGGCGTTGTTTATGATCTTCAACAAGCGCATTGGCACTCACCTTCAGAGCATTCCATCAATGGAAGAAGGTATGACATGGAACTCCACATGGTTCACCAAAGTTCTGATCCCAATGGGAAGAACAAGATTGCTGTTGTTGGTCAACTCTATACAATTGGCAAACCAGACCCTTTTCTCGCACAGTTCAGTAAGCAAATAAGCTACATGATTGATCGAGAGCATGAAATGGGGATTGGAGAGATAGATCCTGCAGATATCAAGTTTGGTGGTAAAAAGTACTACAAATACCTTGGTTCTCTCACTGTTCCTCCCTGTAGTGAAGGTGTAATTTGGGTCATAAACAAGAAGATAAGAACAGTTTCAAAAGAGCAAGTGCGACTTCTGAGAGAAGCTGTTCATGATTATGCAGAGCGCAATGCAAGGCCATTGCAGCCGCTCAATAACCGGGAAGTCCAAGTCTTATCGACCTCTCAAAGATCTAGAACCTGATCACATGGACACCATCTTCATTGAATGTCAAGGTCCCTGTTTTATAATATTTTGGTGAAAAAAAAATATATAAATTTATCACTATCCTTTTTATAAAAAATATTATTATCCATCATCAATGATTTTGTTCTTCATGATAATC

Coding sequence (CDS)

AAGGCATTGGAAAATGACTCGATCAGCGCTTCCCGCCTTCCATGGAAGCGTTATGAACAGCAACTCTCCAAACTTTTTATTCTTCTAGCTTTTCATTCTCAATTGGATCAGAACCTCCCCAGAAATTTCCCACTGCTCTCTGTCTCTCTCTCTCTCGTTGTTTATACATTATATATTGTTCATAATCTGAGAGAATTGACCGAATTTCGACTCCGATTCCATTGCTTTGTCTTGCAAATCCGTTTCCTCGATTCAGTTTTCGCTCCAGAAGCCATGAAAATTGGGACTACACTCATCGTTTTCTTCTCGCTTCTGCTGTTTTCCCTACATTTGTCTTCAATCTCGGCGCAAGAAGTTGAGGATGAGAGGGAATTTGATTACAGAGAAGGGAGTGAGAAAGGGCCAAGACATTGGGGAAAGATAAAGAAAGAGTGGGAAGCTTGTAATAATGGGGTTTTGCAATCTCCCATAGACTTATCCAGCCAGAGAGTGAAGATTGTTCCGAAGTTTGGAGACGTGAAGAGAAGCTACAAACCTTGCAATGCAACGGTCAAAAATAGGGGCCATGACATTTCTATATATTGGGTTGGCAAGGCAGGATCAATCAAGGTAAACGGCGTTGTTTATGATCTTCAACAAGCGCATTGGCACTCACCTTCAGAGCATTCCATCAATGGAAGAAGGTATGACATGGAACTCCACATGGTTCACCAAAGTTCTGATCCCAATGGGAAGAACAAGATTGCTGTTGTTGGTCAACTCTATACAATTGGCAAACCAGACCCTTTTCTCGCACAGTTCAGTAAGCAAATAAGCTACATGATTGATCGAGAGCATGAAATGGGGATTGGAGAGATAGATCCTGCAGATATCAAGTTTGGTGGTAAAAAGTACTACAAATACCTTGGTTCTCTCACTGTTCCTCCCTGTAGTGAAGGTGTAATTTGGGTCATAAACAAGAAGATAAGAACAGTTTCAAAAGAGCAAGTGCGACTTCTGAGAGAAGCTGTTCATGATTATGCAGAGCGCAATGCAAGGCCATTGCAGCCGCTCAATAACCGGGAAGTCCAAGTCTTATCGACCTCTCAAAGATCTAGAACCTGA

Protein sequence

KALENDSISASRLPWKRYEQQLSKLFILLAFHSQLDQNLPRNFPLLSVSLSLVVYTLYIVHNLRELTEFRLRFHCFVLQIRFLDSVFAPEAMKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNGVLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQISYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLREAVHDYAERNARPLQPLNNREVQVLSTSQRSRT
Homology
BLAST of Cp4.1LG10g04430 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 6.5e-74
Identity = 135/263 (51.33%), Postives = 183/263 (69.58%), Query Frame = 0

Query: 99  IVFFSLL-LFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNGVLQSPI 158
           +  FS+  + S+  ++ S  EVEDEREF+Y++  EKGP  WG++K EWE C  G +QSPI
Sbjct: 13  VALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPI 72

Query: 159 DLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDLQQAHWH 218
           DL ++RV IV   G + R Y P NAT+KNRGHDI + +   AG+IK+NG  Y+LQQ HWH
Sbjct: 73  DLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWH 132

Query: 219 SPSEHSINGRRYDMELHMVHQSSDPNGKN-KIAVVGQLYTIGKPDPFLAQFSKQISYMID 278
           SPSEH+INGRR+ +ELHMVH+     G+N ++AVV  LY IG+ D F+    K++  + +
Sbjct: 133 SPSEHTINGRRFALELHMVHE-----GRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAE 192

Query: 279 REH-EMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLRE 338
            E  E  +G IDP  IK G +KYY+Y GSLT PPC++ V W + +K+RTV+++QV+LLR 
Sbjct: 193 MEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRV 252

Query: 339 AVHDYAERNARPLQPLNNREVQV 359
           AVHD A  NARP+QP N R V +
Sbjct: 253 AVHDDANSNARPVQPTNKRIVHL 270

BLAST of Cp4.1LG10g04430 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 247.7 bits (631), Expect = 2.1e-64
Identity = 122/242 (50.41%), Postives = 156/242 (64.46%), Query Frame = 0

Query: 118 EVEDEREFDYREGSEKGPRHWGKIKKEWEACNNGVLQSPIDLSSQRVKIVPKFGDVKRSY 177
           EVEDE EF Y     KGP  WG +  EW+ C  G +QSPIDL  + V +  KFG ++  Y
Sbjct: 133 EVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQY 192

Query: 178 KPCNATVKNRGHDISIYWVG--KAGSIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHM 237
            P N T+KNRGHDI + + G  K   + + G  Y LQQ HWHSPSEH+ING+R+ +E H+
Sbjct: 193 LPSNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFALEEHL 252

Query: 238 VHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQISYMID-REHEMGIGEIDPADIKFG 297
           VH+S D     + AVV  LY +G  DPFL    KQ+  + D    E  +G IDP  + F 
Sbjct: 253 VHESKD----KRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKLSFE 312

Query: 298 GKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLREAVHDYAERNARPLQPLNNR 357
            K YY+Y GSLT PPCSE VIW ++K+IRTVS +QV+LLR AVHD ++ NARPLQ +N R
Sbjct: 313 SKHYYRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKR 370

BLAST of Cp4.1LG10g04430 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.1e-64
Identity = 123/262 (46.95%), Postives = 176/262 (67.18%), Query Frame = 0

Query: 97  TLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNGVLQSP 156
           T ++F S L  S    + S  EV+DE EF Y E SE GP +WG I+ +W+ C +G LQSP
Sbjct: 7   TKMLFISFLFLSSVFLARSG-EVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKLQSP 66

Query: 157 IDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDLQQAHW 216
           ID+   R ++V     +++ YKP NAT+ NRGHDI +  +   G +K+N   Y L+Q HW
Sbjct: 67  IDIFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLR-LDDGGYLKINETQYQLKQLHW 126

Query: 217 HSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGK-PDPFLAQFSKQISYMI 276
           H+PSEH+ING R+++E H+VH+S++     K  V+G +Y IG  PDPFL+     +    
Sbjct: 127 HTPSEHTINGERFNLEAHLVHESNN----GKFVVIGIVYEIGLWPDPFLSMIENDLKVPA 186

Query: 277 DREH-EMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLR 336
           +++  E GIG IDP  IK  GKKY++Y+GSLT PPC+EGV+W+I++K++TV++ Q++LL+
Sbjct: 187 NKKGIERGIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQ 246

Query: 337 EAVHDYAERNARPLQPLNNREV 357
           EAVHD  E NARP QP N R +
Sbjct: 247 EAVHDGFETNARPTQPENERYI 261

BLAST of Cp4.1LG10g04430 vs. ExPASy Swiss-Prot
Match: F4JIK2 (Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.7e-63
Identity = 119/267 (44.57%), Postives = 168/267 (62.92%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSIS--AQEVEDEREFDYREGSEKGPRHWGKIKKEWEACN 151
           M      I F ++    L   +IS    EV+DE  F Y + +EKGP  WGKI   W+ CN
Sbjct: 1   MDTNAKTIFFMAMCFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCN 60

Query: 152 NGVLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVY 211
            G  QSPIDL+++RV ++       R YKP  A + NRGHDI + W G AG + +    +
Sbjct: 61  TGRYQSPIDLTNERVSLIHDQA-WTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDF 120

Query: 212 DLQQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSK 271
           +L Q HWHSPSEH++NG RYD+ELHMVH S+    + + AV+G LY +G+P+ FL +   
Sbjct: 121 NLVQCHWHSPSEHTVNGTRYDLELHMVHTSA----RGRTAVIGVLYKLGEPNEFLTKLLN 180

Query: 272 QISYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQ 331
            I  + ++  E+ +G IDP +I+F  +K+Y+Y+GSLTVPPC+EGVIW + K++ T+S EQ
Sbjct: 181 GIKAVGNK--EINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQ 240

Query: 332 VRLLREAVHDYAERNARPLQPLNNREV 357
           +  LR+AV D  E N+RP+Q    R V
Sbjct: 241 ITALRQAVDDGFETNSRPVQDSKGRSV 260

BLAST of Cp4.1LG10g04430 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.3e-62
Identity = 131/280 (46.79%), Postives = 177/280 (63.21%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQ--EVEDEREFDYREGSEKGPRHWGKIKKEWEACN 151
           MKI +   VFF L+  S+ + S S    EVEDE +F+Y +  EKGP +WG++K EW  C 
Sbjct: 1   MKIPSIGYVFF-LIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCG 60

Query: 152 NGVLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISI-YWVGKAG-SIKVNGV 211
            G +QSPIDL+ +RV I    G ++  Y P NAT+KNRGHDI + +  G AG  I +NG 
Sbjct: 61  KGNMQSPIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGT 120

Query: 212 VYDLQQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQF 271
            Y LQQ HWHSPSEH++NG+R+ +E HMVHQS D  G+N  AVV   Y +GKPD FL   
Sbjct: 121 EYKLQQIHWHSPSEHTLNGKRFVLEEHMVHQSKD--GRN--AVVAFFYKLGKPDYFLLTL 180

Query: 272 SKQISYMID-REHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVS 331
            + +  + D  E +  +  + P    F  K YY+++GSLT PPCSE VIW I+K++RTV+
Sbjct: 181 ERYLKRITDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVT 240

Query: 332 KEQVRLLREAVHDYAERNARPLQPLNNREVQVLSTSQRSR 367
            +Q+ +LR  VHD +  NARPLQ  N R V +   +  S+
Sbjct: 241 LKQLIMLRVTVHDQSNSNARPLQRKNERPVALYIPTWHSK 275

BLAST of Cp4.1LG10g04430 vs. NCBI nr
Match: XP_022977815.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 620 bits (1600), Expect = 1.42e-222
Identity = 310/345 (89.86%), Postives = 326/345 (94.49%), Query Frame = 0

Query: 25  LFILLAFHSQLDQNLPRNFPLLSVSLSLV----VYTLYIVHNLRELTEFRLRFHCFVLQI 84
           +F+LL FHS L QNLPRNFPLLSVSLSL     VYTLYIVHNLRE TEF+LRFHCFVLQI
Sbjct: 1   MFLLLDFHSPLHQNLPRNFPLLSVSLSLSLSLDVYTLYIVHNLREFTEFQLRFHCFVLQI 60

Query: 85  RFLDSVFAPEAMKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGK 144
           RFLDSVFAPEAMKI TTLIVFFSLLLFSLHLSSISAQEVEDE EFDYREGSEKGPRHWGK
Sbjct: 61  RFLDSVFAPEAMKIETTLIVFFSLLLFSLHLSSISAQEVEDESEFDYREGSEKGPRHWGK 120

Query: 145 IKKEWEACNNGVLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAG 204
           IKKEWEACNNGVLQSPIDLSS+RVKIVPKFGD+KRSYKPCNAT+KNRGHDIS+YWVGKAG
Sbjct: 121 IKKEWEACNNGVLQSPIDLSSERVKIVPKFGDLKRSYKPCNATIKNRGHDISVYWVGKAG 180

Query: 205 SIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKP 264
           SIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMVHQSS+PN KNKIAVVGQLYTIG+P
Sbjct: 181 SIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMVHQSSNPNVKNKIAVVGQLYTIGEP 240

Query: 265 DPFLAQFSKQISYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINK 324
           +PFLAQFSKQIS MIDREHE+GIGEIDPADIKFGGKKYYKYLGSLTVPPC+EGVIW INK
Sbjct: 241 EPFLAQFSKQISSMIDREHEVGIGEIDPADIKFGGKKYYKYLGSLTVPPCTEGVIWAINK 300

Query: 325 KIRTVSKEQVRLLREAVHDYAERNARPLQPLNNREVQVLSTSQRS 365
           KIRTVS EQV+LLREAVHDYA+RN RPLQ LNNREV +LSTS+ S
Sbjct: 301 KIRTVSHEQVQLLREAVHDYADRNVRPLQRLNNREVNILSTSRGS 345

BLAST of Cp4.1LG10g04430 vs. NCBI nr
Match: XP_023544237.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558 bits (1439), Expect = 3.70e-199
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 151
           MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG
Sbjct: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 60

Query: 152 VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 211
           VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL
Sbjct: 61  VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120

Query: 212 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 271
           QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI
Sbjct: 121 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 180

Query: 272 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 331
           SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR
Sbjct: 181 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240

Query: 332 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRSRT 367
           LLREAVHDYAERNARPLQPLNNREVQVLSTSQRSRT
Sbjct: 241 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRSRT 276

BLAST of Cp4.1LG10g04430 vs. NCBI nr
Match: XP_022925725.1 (alpha carbonic anhydrase 7-like [Cucurbita moschata])

HSP 1 Score: 535 bits (1377), Expect = 9.56e-190
Identity = 263/274 (95.99%), Postives = 267/274 (97.45%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 151
           MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEAC+NG
Sbjct: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60

Query: 152 VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 211
           VLQSPIDLSSQRVKIVPK G +KRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL
Sbjct: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120

Query: 212 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 271
           QQAHWHSPSEHSINGRRYDMELHM HQSSDPNGKNKIAVVGQLYT GKPDPFLAQFSKQI
Sbjct: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180

Query: 272 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 331
           SYMIDREHEMGIGEIDPADIKFGG KYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR
Sbjct: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240

Query: 332 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRS 365
           LLREAVHDYAERNARPLQPLNNREV ++STS RS
Sbjct: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 274

BLAST of Cp4.1LG10g04430 vs. NCBI nr
Match: KAG6604394.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034544.1 Alpha carbonic anhydrase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 534 bits (1376), Expect = 1.36e-189
Identity = 262/274 (95.62%), Postives = 267/274 (97.45%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 151
           MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEAC+NG
Sbjct: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60

Query: 152 VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 211
           VLQSPIDLSSQRVKIVPK G +KRSYKPCNATVKNRGHDISIYWVGKAGS+KVNGVVYDL
Sbjct: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSVKVNGVVYDL 120

Query: 212 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 271
           QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYT GKPDPFLAQF KQI
Sbjct: 121 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFCKQI 180

Query: 272 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 331
           SYMIDREHEMGIGEIDPADIKFGG KYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR
Sbjct: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240

Query: 332 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRS 365
           LLREAVHDYAERNARPLQPLNNREV ++STS RS
Sbjct: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 274

BLAST of Cp4.1LG10g04430 vs. NCBI nr
Match: XP_038881410.1 (alpha carbonic anhydrase 7-like [Benincasa hispida])

HSP 1 Score: 453 bits (1165), Expect = 1.92e-157
Identity = 218/275 (79.27%), Postives = 246/275 (89.45%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 151
           MKI T  IVF S+LLF LH S IS+QEVEDEREFDY EGSEKGP HWGK+KKEWEACNNG
Sbjct: 1   MKIWTKPIVFLSILLFFLHSSLISSQEVEDEREFDYMEGSEKGPGHWGKLKKEWEACNNG 60

Query: 152 VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 211
            LQSPIDLSS RVKI+PK G++KRSY PCNATVKNRGHDISIYWVGK GSI+VNGVVYDL
Sbjct: 61  DLQSPIDLSSLRVKIIPKLGELKRSYYPCNATVKNRGHDISIYWVGKPGSIQVNGVVYDL 120

Query: 212 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 271
           QQ+HWHSPSEHSINGRRYD+ELHMVH SSDP  KNKIAVVGQLYTIG+PDPFL+Q S++I
Sbjct: 121 QQSHWHSPSEHSINGRRYDLELHMVHLSSDPTVKNKIAVVGQLYTIGQPDPFLSQLSREI 180

Query: 272 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 331
           S M+DR+HE  +G ++PADIKFGG+KYY+YLGSLTVPPC+EGVIW +NKKIRTVS EQV+
Sbjct: 181 SSMVDRKHEKKVGVMNPADIKFGGRKYYRYLGSLTVPPCTEGVIWTMNKKIRTVSMEQVK 240

Query: 332 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRSR 366
           LLREAVHDYAE NARPLQPLN+RE+ +   +QR+R
Sbjct: 241 LLREAVHDYAEFNARPLQPLNSREISLYRPTQRTR 275

BLAST of Cp4.1LG10g04430 vs. ExPASy TrEMBL
Match: A0A6J1INE0 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111478003 PE=3 SV=1)

HSP 1 Score: 620 bits (1600), Expect = 6.89e-223
Identity = 310/345 (89.86%), Postives = 326/345 (94.49%), Query Frame = 0

Query: 25  LFILLAFHSQLDQNLPRNFPLLSVSLSLV----VYTLYIVHNLRELTEFRLRFHCFVLQI 84
           +F+LL FHS L QNLPRNFPLLSVSLSL     VYTLYIVHNLRE TEF+LRFHCFVLQI
Sbjct: 1   MFLLLDFHSPLHQNLPRNFPLLSVSLSLSLSLDVYTLYIVHNLREFTEFQLRFHCFVLQI 60

Query: 85  RFLDSVFAPEAMKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGK 144
           RFLDSVFAPEAMKI TTLIVFFSLLLFSLHLSSISAQEVEDE EFDYREGSEKGPRHWGK
Sbjct: 61  RFLDSVFAPEAMKIETTLIVFFSLLLFSLHLSSISAQEVEDESEFDYREGSEKGPRHWGK 120

Query: 145 IKKEWEACNNGVLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAG 204
           IKKEWEACNNGVLQSPIDLSS+RVKIVPKFGD+KRSYKPCNAT+KNRGHDIS+YWVGKAG
Sbjct: 121 IKKEWEACNNGVLQSPIDLSSERVKIVPKFGDLKRSYKPCNATIKNRGHDISVYWVGKAG 180

Query: 205 SIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKP 264
           SIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMVHQSS+PN KNKIAVVGQLYTIG+P
Sbjct: 181 SIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMVHQSSNPNVKNKIAVVGQLYTIGEP 240

Query: 265 DPFLAQFSKQISYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINK 324
           +PFLAQFSKQIS MIDREHE+GIGEIDPADIKFGGKKYYKYLGSLTVPPC+EGVIW INK
Sbjct: 241 EPFLAQFSKQISSMIDREHEVGIGEIDPADIKFGGKKYYKYLGSLTVPPCTEGVIWAINK 300

Query: 325 KIRTVSKEQVRLLREAVHDYAERNARPLQPLNNREVQVLSTSQRS 365
           KIRTVS EQV+LLREAVHDYA+RN RPLQ LNNREV +LSTS+ S
Sbjct: 301 KIRTVSHEQVQLLREAVHDYADRNVRPLQRLNNREVNILSTSRGS 345

BLAST of Cp4.1LG10g04430 vs. ExPASy TrEMBL
Match: A0A6J1EJ09 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111433054 PE=3 SV=1)

HSP 1 Score: 535 bits (1377), Expect = 4.63e-190
Identity = 263/274 (95.99%), Postives = 267/274 (97.45%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 151
           MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEAC+NG
Sbjct: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60

Query: 152 VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 211
           VLQSPIDLSSQRVKIVPK G +KRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL
Sbjct: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120

Query: 212 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 271
           QQAHWHSPSEHSINGRRYDMELHM HQSSDPNGKNKIAVVGQLYT GKPDPFLAQFSKQI
Sbjct: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180

Query: 272 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 331
           SYMIDREHEMGIGEIDPADIKFGG KYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR
Sbjct: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240

Query: 332 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRS 365
           LLREAVHDYAERNARPLQPLNNREV ++STS RS
Sbjct: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 274

BLAST of Cp4.1LG10g04430 vs. ExPASy TrEMBL
Match: A0A0A0KKA6 (Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_6G446560 PE=3 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 3.58e-155
Identity = 212/276 (76.81%), Postives = 246/276 (89.13%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 151
           M+  +T IVFF +LL+ +H SSISAQEVEDEREFDY EGS+KGP HWG+IKKEWEACNNG
Sbjct: 1   MQFCSTPIVFFFILLYFIHSSSISAQEVEDEREFDYAEGSQKGPGHWGEIKKEWEACNNG 60

Query: 152 VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 211
            LQSPIDLSSQRVKIVP+ G++KRSY PCNATVKNRGHDIS+YW GK GSI++NG VYDL
Sbjct: 61  DLQSPIDLSSQRVKIVPQLGELKRSYYPCNATVKNRGHDISVYWYGKPGSIEINGDVYDL 120

Query: 212 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 271
           QQ+HWHSPSEHS+NGRRYD+ELHMVHQSSDP  KNKIAVVGQLYTIG+PDPF+ Q S++I
Sbjct: 121 QQSHWHSPSEHSVNGRRYDLELHMVHQSSDPTVKNKIAVVGQLYTIGQPDPFITQLSREI 180

Query: 272 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 331
             M+DR+HE  +G ++PADIKFGGKKYY+YLGSLTVPPC+EGVIW +NKKIRTVS+EQVR
Sbjct: 181 LGMVDRKHEKKVGVMNPADIKFGGKKYYRYLGSLTVPPCTEGVIWTMNKKIRTVSREQVR 240

Query: 332 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRSRT 367
           LLREAVHDYAE NARPLQPLN+RE+ +    QR+R+
Sbjct: 241 LLREAVHDYAEFNARPLQPLNSREIGLYRPIQRTRS 276

BLAST of Cp4.1LG10g04430 vs. ExPASy TrEMBL
Match: A0A1S3CIK8 (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103500831 PE=3 SV=1)

HSP 1 Score: 443 bits (1140), Expect = 5.90e-154
Identity = 211/275 (76.73%), Postives = 245/275 (89.09%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 151
           MK  T  I+FF +LLF LH SSISAQEVEDER+FDY E S+KGP HWG+IKKEWEACNNG
Sbjct: 1   MKFCTKPILFFFILLFFLHSSSISAQEVEDERDFDYVEWSQKGPGHWGEIKKEWEACNNG 60

Query: 152 VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 211
            LQSPIDLSS+RVKIVP+ G++KR+Y PCNATVKNRGHDIS+YW GK GSI++NGVVYDL
Sbjct: 61  DLQSPIDLSSERVKIVPQLGELKRNYYPCNATVKNRGHDISVYWYGKPGSIEINGVVYDL 120

Query: 212 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 271
           QQ+HWHSPSEHSINGRRYD+ELHMVHQSSDP  KNKIAVVGQLYTIG+PDPF+ Q S++I
Sbjct: 121 QQSHWHSPSEHSINGRRYDLELHMVHQSSDPTVKNKIAVVGQLYTIGQPDPFITQLSREI 180

Query: 272 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 331
             M+DR+HE  +G ++PADIKFGG+KYY+YLGSLTVPPC+EGVIW +NKKIRTVS+EQVR
Sbjct: 181 LGMVDRKHEKKVGVMNPADIKFGGRKYYRYLGSLTVPPCTEGVIWTMNKKIRTVSREQVR 240

Query: 332 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRSR 366
           LLREAVHDYAE NARPLQPLN+RE+ +   +QR+R
Sbjct: 241 LLREAVHDYAEFNARPLQPLNSREIGLYRPTQRTR 275

BLAST of Cp4.1LG10g04430 vs. ExPASy TrEMBL
Match: A0A6J1CS77 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111013713 PE=3 SV=1)

HSP 1 Score: 441 bits (1133), Expect = 6.85e-153
Identity = 212/275 (77.09%), Postives = 241/275 (87.64%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 151
           MKI T   VFFS+LLF LH SSISAQEVE+EREFDY EGS+KGP HWGK+KKEWEACNNG
Sbjct: 1   MKIRTKHFVFFSILLFFLHSSSISAQEVENEREFDYIEGSDKGPGHWGKLKKEWEACNNG 60

Query: 152 VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 211
            LQSPIDL+SQRVKI+PK G++ RSY PCNAT+KNRGHDISIYWVG AGSI VNGVVYDL
Sbjct: 61  DLQSPIDLTSQRVKIIPKLGELNRSYYPCNATIKNRGHDISIYWVGNAGSILVNGVVYDL 120

Query: 212 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 271
           QQ+HWHSPSEHSING RYD+ELHMVH SSDPN KNKIAVVGQLY IG PDPFL+Q  + I
Sbjct: 121 QQSHWHSPSEHSINGVRYDLELHMVHLSSDPNVKNKIAVVGQLYKIGPPDPFLSQLGRDI 180

Query: 272 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 331
           S MID++HE  +G ++PADIKFGG+KYY+YLGSLTVPPC+EGVIW +NKKIRTVS+EQV+
Sbjct: 181 SGMIDQKHEKKVGVMNPADIKFGGRKYYRYLGSLTVPPCTEGVIWTMNKKIRTVSREQVK 240

Query: 332 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRSR 366
           LLREAVHDYAE NARPLQPLN+R + +    QR++
Sbjct: 241 LLREAVHDYAEFNARPLQPLNSRHIHLYRPIQRTK 275

BLAST of Cp4.1LG10g04430 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 279.3 bits (713), Expect = 4.6e-75
Identity = 135/263 (51.33%), Postives = 183/263 (69.58%), Query Frame = 0

Query: 99  IVFFSLL-LFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNGVLQSPI 158
           +  FS+  + S+  ++ S  EVEDEREF+Y++  EKGP  WG++K EWE C  G +QSPI
Sbjct: 13  VALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPI 72

Query: 159 DLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDLQQAHWH 218
           DL ++RV IV   G + R Y P NAT+KNRGHDI + +   AG+IK+NG  Y+LQQ HWH
Sbjct: 73  DLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWH 132

Query: 219 SPSEHSINGRRYDMELHMVHQSSDPNGKN-KIAVVGQLYTIGKPDPFLAQFSKQISYMID 278
           SPSEH+INGRR+ +ELHMVH+     G+N ++AVV  LY IG+ D F+    K++  + +
Sbjct: 133 SPSEHTINGRRFALELHMVHE-----GRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAE 192

Query: 279 REH-EMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLRE 338
            E  E  +G IDP  IK G +KYY+Y GSLT PPC++ V W + +K+RTV+++QV+LLR 
Sbjct: 193 MEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRV 252

Query: 339 AVHDYAERNARPLQPLNNREVQV 359
           AVHD A  NARP+QP N R V +
Sbjct: 253 AVHDDANSNARPVQPTNKRIVHL 270

BLAST of Cp4.1LG10g04430 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 242.3 bits (617), Expect = 6.2e-64
Identity = 119/267 (44.57%), Postives = 168/267 (62.92%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSIS--AQEVEDEREFDYREGSEKGPRHWGKIKKEWEACN 151
           M      I F ++    L   +IS    EV+DE  F Y + +EKGP  WGKI   W+ CN
Sbjct: 1   MDTNAKTIFFMAMCFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCN 60

Query: 152 NGVLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVY 211
            G  QSPIDL+++RV ++       R YKP  A + NRGHDI + W G AG + +    +
Sbjct: 61  TGRYQSPIDLTNERVSLIHDQA-WTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDF 120

Query: 212 DLQQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSK 271
           +L Q HWHSPSEH++NG RYD+ELHMVH S+    + + AV+G LY +G+P+ FL +   
Sbjct: 121 NLVQCHWHSPSEHTVNGTRYDLELHMVHTSA----RGRTAVIGVLYKLGEPNEFLTKLLN 180

Query: 272 QISYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQ 331
            I  + ++  E+ +G IDP +I+F  +K+Y+Y+GSLTVPPC+EGVIW + K++ T+S EQ
Sbjct: 181 GIKAVGNK--EINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQ 240

Query: 332 VRLLREAVHDYAERNARPLQPLNNREV 357
           +  LR+AV D  E N+RP+Q    R V
Sbjct: 241 ITALRQAVDDGFETNSRPVQDSKGRSV 260

BLAST of Cp4.1LG10g04430 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 240.4 bits (612), Expect = 2.4e-63
Identity = 131/280 (46.79%), Postives = 177/280 (63.21%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLLLFSLHLSSISAQ--EVEDEREFDYREGSEKGPRHWGKIKKEWEACN 151
           MKI +   VFF L+  S+ + S S    EVEDE +F+Y +  EKGP +WG++K EW  C 
Sbjct: 1   MKIPSIGYVFF-LIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCG 60

Query: 152 NGVLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISI-YWVGKAG-SIKVNGV 211
            G +QSPIDL+ +RV I    G ++  Y P NAT+KNRGHDI + +  G AG  I +NG 
Sbjct: 61  KGNMQSPIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGT 120

Query: 212 VYDLQQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQF 271
            Y LQQ HWHSPSEH++NG+R+ +E HMVHQS D  G+N  AVV   Y +GKPD FL   
Sbjct: 121 EYKLQQIHWHSPSEHTLNGKRFVLEEHMVHQSKD--GRN--AVVAFFYKLGKPDYFLLTL 180

Query: 272 SKQISYMID-REHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVS 331
            + +  + D  E +  +  + P    F  K YY+++GSLT PPCSE VIW I+K++RTV+
Sbjct: 181 ERYLKRITDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVT 240

Query: 332 KEQVRLLREAVHDYAERNARPLQPLNNREVQVLSTSQRSR 367
            +Q+ +LR  VHD +  NARPLQ  N R V +   +  S+
Sbjct: 241 LKQLIMLRVTVHDQSNSNARPLQRKNERPVALYIPTWHSK 275

BLAST of Cp4.1LG10g04430 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 226.1 bits (575), Expect = 4.6e-59
Identity = 106/208 (50.96%), Postives = 140/208 (67.31%), Query Frame = 0

Query: 119 VEDEREFDYREGSEKGPRHWGKIKKEWEACNNGVLQSPIDLSSQRVKIVPKFGDVKRSYK 178
           VEDE EF Y    E GP  WGK++ EW+ C  G +QSPIDL ++RV++V     + R YK
Sbjct: 5   VEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYK 64

Query: 179 PCNATVKNRGHDISI-YWVGKAGSIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMVH 238
           PCNAT+KNRGHD+ + +    +GSI VNG  Y L Q HWHSPSEH++NGRR+ +ELHMVH
Sbjct: 65  PCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVH 124

Query: 239 QSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQISYMIDR-EHEMGIGEIDPADIKFGGK 298
           +    N    +AVV  LY IG+PD FL     ++S + D+ E E  +  IDP DIK G +
Sbjct: 125 E----NINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSR 184

Query: 299 KYYKYLGSLTVPPCSEGVIWVINKKIRT 325
           K+Y+Y+GSLT PPC++ VIW + KK+ T
Sbjct: 185 KFYRYIGSLTTPPCTQNVIWTVVKKVNT 208

BLAST of Cp4.1LG10g04430 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 214.2 bits (544), Expect = 1.8e-55
Identity = 100/234 (42.74%), Postives = 151/234 (64.53%), Query Frame = 0

Query: 92  MKIGTTLIVFFSLL---LFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEAC 151
           M   T  I+FF +    LFS ++  + A+E+ ++  F Y++ +EKGP  WGK+  +W+ C
Sbjct: 1   MDANTKTILFFVVFFIDLFSPNILFVYAREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVC 60

Query: 152 NNGVLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVV 211
           + G +QSPIDL+ +RV ++     + + YKP +A +++RGHD+ + W G  G I ++   
Sbjct: 61  STGKIQSPIDLTDERVSLIHDQA-LSKHYKPASAVIQSRGHDVMVSWKGDGGKITIHQTD 120

Query: 212 YDLQQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFS 271
           Y L Q HWHSPSEH+ING  YD+ELHMVH S+      K  VVG LY +G+PD FL +  
Sbjct: 121 YKLVQCHWHSPSEHTINGTSYDLELHMVHTSA----SGKTTVVGVLYKLGEPDEFLTKIL 180

Query: 272 KQISYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKI 323
             I  +  +E ++GI  +DP DI+F    +Y+Y+GSLT+PPC+EGVIW + K++
Sbjct: 181 NGIKGVGKKEIDLGI--VDPRDIRFETNNFYRYIGSLTIPPCTEGVIWTVQKRV 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8176.5e-7451.33Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1[more]
Q9FM992.1e-6450.41Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3[more]
Q84UV82.1e-6446.95Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS... [more]
F4JIK28.7e-6344.57Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1[more]
F4HUC43.3e-6246.79Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022977815.11.42e-22289.86alpha carbonic anhydrase 7-like [Cucurbita maxima][more]
XP_023544237.13.70e-199100.00alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022925725.19.56e-19095.99alpha carbonic anhydrase 7-like [Cucurbita moschata][more]
KAG6604394.11.36e-18995.62Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_038881410.11.92e-15779.27alpha carbonic anhydrase 7-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1INE06.89e-22389.86Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111478003 PE=3 SV=1[more]
A0A6J1EJ094.63e-19095.99Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111433054 PE=3 SV=1[more]
A0A0A0KKA63.58e-15576.81Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_6G446560 PE=3 SV=1[more]
A0A1S3CIK85.90e-15476.73Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103500831 PE=3 SV=1[more]
A0A6J1CS776.85e-15377.09Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111013713 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08080.14.6e-7551.33alpha carbonic anhydrase 7 [more]
AT4G20990.16.2e-6444.57alpha carbonic anhydrase 4 [more]
AT1G08065.12.4e-6346.79alpha carbonic anhydrase 5 [more]
AT2G28210.14.6e-5950.96alpha carbonic anhydrase 2 [more]
AT4G21000.11.8e-5542.74alpha carbonic anhydrase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001148Alpha carbonic anhydrase domainSMARTSM01057Carb_anhydrase_2acoord: 127..360
e-value: 1.3E-64
score: 230.7
IPR001148Alpha carbonic anhydrase domainPFAMPF00194Carb_anhydrasecoord: 126..357
e-value: 8.7E-43
score: 146.7
IPR001148Alpha carbonic anhydrase domainPROSITEPS51144ALPHA_CA_2coord: 123..360
score: 45.507305
IPR036398Alpha carbonic anhydrase domain superfamilyGENE3D3.10.200.10Alpha carbonic anhydrasecoord: 121..365
e-value: 1.2E-74
score: 252.8
IPR036398Alpha carbonic anhydrase domain superfamilySUPERFAMILY51069Carbonic anhydrasecoord: 124..356
IPR023561Carbonic anhydrase, alpha-classPANTHERPTHR18952CARBONIC ANHYDRASEcoord: 98..363
NoneNo IPR availablePANTHERPTHR18952:SF201ALPHA CARBONIC ANHYDRASE 3coord: 98..363
IPR018338Carbonic anhydrase, alpha-class, conserved sitePROSITEPS00162ALPHA_CA_1coord: 220..236
IPR041891Carbonic anhydrase, prokaryotic-likeCDDcd03124alpha_CA_prokaryotic_likecoord: 134..356
e-value: 5.89369E-90
score: 267.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g04430.1Cp4.1LG10g04430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006730 one-carbon metabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding