Cucsat.G21374 (gene) Cucumber (B10) v3

Overview
NameCucsat.G21374
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionprotein HAPLESS 2
Locationctg910: 1989181 .. 1993113 (+)
RNA-Seq ExpressionCucsat.G21374
SyntenyCucsat.G21374
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGAAGTGGAAGAAAACTCCACAAACAAGATGCAAACTCTGAGAACACCCCCGGTTTTGACTGTCAGCAAATCACCCGCTTATGTTTTGTATGAGCTAACATACATTCGTGTAATTCCCGCTTATGATTCTGTTTCCTTGTATCTAATTTTAATAATATGTTTGTGGTTACTGTGGTGTGGTGAAGTTCTAATCTGAATGTTTCATGTTTATGAAGTTTGATACTTGTATATATTTTTTATGTTTGAACACAATATGCTCAGGATGTTCCATATAAGCCTGAAGAATTTTATGTTCCAACTCGCAAATGCGAGCCGGATGCTAGCGCAAGAGTGGTACAAATATGTGAGAGGTAAGATAAGCCAGTCTTTTTAACTTGTTTTCCACGCAACCCCAAAGGTATTTGATTTACATTATTTTGTTTGATGTTTTCTTGTAGGTTAAGAGATGAAAGTGGACATATAATTCTGAGCACTCAAGTACCAATTTGAGAAAAGCTTCTATTGTCTTTGACTTTCTTTGGTTATATTGATTGATCTTCAGTCTTCACGTTCATTTTGATCTTGTTATTGATTCATTCTTTTTTGTTTCTTGTTAAATCTGTTTAGCCAATATGTTGTCCTTGTGGGGCAAAGCGTCGAATGCCTACGTCGTGTGGGAACTTTTGTAAGTTCGTTTTCCGTTTCTCCGGTTGCTAGTTCCTTCAAACACTGATTTGTGATCTTAACTCTTTTATTTCTAAGTCGACAAGATGATTAAAGGAAAGGCAAACACTGCACATTGTCTACGTTTTCCCGGTGACTGGTATGACTTATGTTTTCAGCTTTGTTTTTTGTCATTTTTTCCTTTTAATACTGATATATAGTAGTACTAGCAACCCCTTCTAAGAACATAACCTTATCATGAGGTCTTGATATTTATACTTTACCACCTTGATCTTAAACCGGTTTAGAAGCTTAACCATCATTATTATTATTCTTTTATTTACATTAAAGAGTGGATGTTTGAACCTCAAACTAAAATCCATTCAGTTAAGTTCTTGATAATTAACTTGTCATTCCAAAAAGCATACTTTTGGAGTTGCATTAATCAACTCATGTGTTACTTTAATGTATGTTGATATATTTAAGTATGTATCATATACATTAATGTAGGATGGTTGGATGAATTTGAAGTTGATGAAACTCAGCTTAGAACCTCCTTAAAGTGTACTACTAGCAAAAGATTCTAACTTAGAGTTTCTATCTCACTTAGGTTTCATGTTTTTAGTATTGGACAATGGACATTGGGATTCAGTGTTCAGATTCATGTGAAGTCAGGATCTAAAGTTTCGGTATGTTTTCCTCGATACCCAATGGAGAAAACTTCAATATTATCACTGTCTCATTCTCAAGTTTTGATCAAAATTTAGTGATTGACATTAAATTTAATTTTTTAGGAAGTGTCTGTGGGTCCTGAAAATAGAACAGTGGTGTCAAATGATAACTTCTTACGGGCTAATCTTATTGGTGACCTTGTTGGATACACAAATATTCCATCATTTGAGGACTTTTACCTTGTTATTCCCAGGCAGGTATATGCTTATTCCACCACAAAAGCATTTGATGGAACATTTTGTAATCGAAGATTTTTAGACATTAATGCGTACAACGGAAAGGCTACCTTGATATATATAACTTTGCTTGTTTGTTCTTATCTTCTGTTGCTAAGCTTAAAATATTCAATTGTGATGGAAGGGTGGTCCTGGTCAACCACAGAATTTAGGTACCAATTTCTCTATGTGGATGCTACTAGAAAGAGTGAGATTTACTTTAGATGGTCTTGAATGCAACAAAATTGGTGTTGGTTATGAGACTTTTAATGGACAGCCTGATTTCTGTACATCCCCATTTTGGAGTTGTTTGCACAACCAATTATGGAATTTCAGGGAGGTAAGCTTTTAATCAAGAAAGGGTACTACTAACATTGATTGCTTTTATATGATCTGATACGTTTTCCCAATTTTCGAAAGGCCGATTTGAGTCGAATTGGTAGGAAACAATTGCCATTATATGGAGTGGAAGGACGGTTTGAGAGGATTAATCAACATCCAGTTAAGTATTGGAGGATATAGAATTATTCTTCATTCATGAGTATTCATATTGCTAAACGTTTTGTGTTGGGTGGGTTGAAGAATGCTGGGACACATTCGTTCTCCATTGGAGTCACTGAAGTTCTCAATACAAATCTTGTAATAGAATTGCGTGCTGATGATGTTGAGTATGTTTACCAAAGGTGAGTTGTTAGCACTTCTTGTAATTAAACTGCATCCCGAATGTGACTTGGTATGAATACGCTACGATACATGTTTAAGTGTCATTGTGCTAACAAGTGACCAATATATGTCCAACAAGCGTCGAAGTGTCTAAGTGCTTGCCAAACTTAAGTGTCCGTGTTTTGAACCAAATATATATATTTTTTTGAAGATATGAAAAGCGATTAGCAATCACATATGTTCTTCAATAGGCTACATTTTATTTAATGTAGGAGTCCTGGGAAAATTATGAGCATCAGCATCCCAACTTTTGAAGCCCTCACACAATTTGGAGTTGCTACAGTTGCAACTAAGAACACTGGAGAAGTGGAAGCATCTTATAGCTTAACGGTATTTACTAAGCTTTTACTACAATGAGATATATGGTTGGAATACTTGAGTGAAGCTTTTATTGTTTCTTTTGATCTCCTGCACATTATTTCAACTGGATGGATTTTTCTTTCTTGCAGTTTACTTGTTCAAAAGAAGTCAGTCTCATGGAGGTACCGTCTTTCGTGGAGACATTTTGATAGAATTTTGTTCATGTGATGAGTTTTTGATGAAAATCTAGATGATTTCAGGAACAATATTATATCATGAAGCCAAATGAAATTGCTAGCCGTTCATTTAAACTCTACCCAACTACTGATCAAGCAGCAAAATATGTTTGTGCTGGTAAGATGAGTAACTAATTTGTGCTTCTTTTCTCATTAAATTTATTTAGTTCAGATTCAAGATTCTTTGAGACAAAACTTCTCTTTGTTGATAGTGCAGCCATACTTAAGGATGCTGATTTTAGTGAAGTTGATAGAGCTGAATGCCAATTTGCTACTACTGCTACTGTCCTTGACAATGGATCACAGGTTCCTTTTACAGTTCTAGAATCATGGTGAATCAAGTATCCTTCTTCCATTAGTGAGTTTGGCTTTTTTTTTTTTTTTTTAACAGATTACTCCTTTTGAACTCCCCAAGAAAAAGGAAAATGGTTTCATCCATTCAATCAAGCTCGCCTGGAAGCAGTTTTGGGGAAGCGTCATTGACTTTGTCACTGGGAAATCTTGCAGGTATTTGCTATCAAACTCTCATAAACCATTTACCTTCCATTGACATACCATCCCCAATGACACGTTTGCCTCTTTTGAACAGAAAAGTGTGCTCTGGATTTTTTGACTTCAGCTGTCACATACAATATATATGTTTGAGTTGGCTTGTGTTGTTTGGTCTCTTTTTGGCCACTTTCCCCGCAGGTAACTATTATCCAACACTTGAAAAGTGATTTCAAACAAGTCGGCCTAAAATTAAAAATAAGCATCTCATCTTCATAATTTGTTTCAATTACTATGCAGTACTTGTGATACTGTGGGTTTTACATCAGAAAGGCTTATTTGACCCTCTTTATGACTGGTGGGAGGATATGTTTTGTCACAAAAGTGAGCCCACAAGGTCCACTTGGAAGTATAGAGGTGAAAGAAAACATTACCATAGACATGGTAGTAGGCATCACCAAAATCATGGGAGTGGATACAAGAGAAGAAGCCATGAATTACACAAAAAGCACAAACATTCTGAGAGAGACACAGATTACTTTCTTCACCATGTGCACAGGAAAAAAGGTAAGAGGGGACACAATAGAGTGTAGAA

Coding sequence (CDS)

GAAGTGGAAGAAAACTCCACAAACAAGATGCAAACTCTGAGAACACCCCCGGTTTTGACTGTCAGCAAATCACCCGCTTATGTTTTGTATGAGCTAACATACATTCGTGATGTTCCATATAAGCCTGAAGAATTTTATGTTCCAACTCGCAAATGCGAGCCGGATGCTAGCGCAAGAGTGGTACAAATATGTGAGAGGTTAAGAGATGAAAGTGGACATATAATTCTGAGCACTCAACCAATATGTTGTCCTTGTGGGGCAAAGCGTCGAATGCCTACGTCGTGTGGGAACTTTTTCGACAAGATGATTAAAGGAAAGGCAAACACTGCACATTGTCTACGTTTTCCCGGTGACTGGTTTCATGTTTTTAGTATTGGACAATGGACATTGGGATTCAGTGTTCAGATTCATGTGAAGTCAGGATCTAAAGTTTCGGAAGTGTCTGTGGGTCCTGAAAATAGAACAGTGGTGTCAAATGATAACTTCTTACGGGCTAATCTTATTGGTGACCTTGTTGGATACACAAATATTCCATCATTTGAGGACTTTTACCTTGTTATTCCCAGGCAGGGTGGTCCTGGTCAACCACAGAATTTAGGTACCAATTTCTCTATGTGGATGCTACTAGAAAGAGTGAGATTTACTTTAGATGGTCTTGAATGCAACAAAATTGGTGTTGGTTATGAGACTTTTAATGGACAGCCTGATTTCTGTACATCCCCATTTTGGAGTTGTTTGCACAACCAATTATGGAATTTCAGGGAGGCCGATTTGAGTCGAATTGGTAGGAAACAATTGCCATTATATGGAGTGGAAGGACGGTTTGAGAGGATTAATCAACATCCAAATGCTGGGACACATTCGTTCTCCATTGGAGTCACTGAAGTTCTCAATACAAATCTTGTAATAGAATTGCGTGCTGATGATGTTGAGTATGTTTACCAAAGGAGTCCTGGGAAAATTATGAGCATCAGCATCCCAACTTTTGAAGCCCTCACACAATTTGGAGTTGCTACAGTTGCAACTAAGAACACTGGAGAAGTGGAAGCATCTTATAGCTTAACGTTTACTTGTTCAAAAGAAGTCAGTCTCATGGAGGAACAATATTATATCATGAAGCCAAATGAAATTGCTAGCCGTTCATTTAAACTCTACCCAACTACTGATCAAGCAGCAAAATATGTTTGTGCTGTGCAGCCATACTTAAGGATGCTGATTTTAGTGAAGTTGATAGAGCTGAATGCCAATTTGCTACTACTGCTACTGTCCTTGACAATGGATCACAGATTACTCCTTTTGAACTCCCCAAGAAAAAGGAAAATGGTTTCATCCATTCAATCAAGCTCGCCTGGAAGCAGTTTTGGGGAAGCGTCATTGACTTTGTCACTGGGAAATCTTGCAGAAAAGTGTGCTCTGGATTTTTTGACTTCAGCTGTCACATACAATATATATGTTTGA

Protein sequence

EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARVVQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWFHVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSFEDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAVQPYLRMLILVKLIELNANLLLLLLSLTMDHRLLLLNSPRKRKMVSSIQSSSPGSSFGEASLTLSLGNLAEKCALDFLTSAVTYNIYV
Homology
BLAST of Cucsat.G21374 vs. ExPASy Swiss-Prot
Match: F4JP36 (Protein HAPLESS 2 OS=Arabidopsis thaliana OX=3702 GN=HAP2 PE=1 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 2.0e-187
Identity = 300/397 (75.57%), Postives = 352/397 (88.66%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVE+NS++ MQT+R PPV+TV+KS AY LY+LTYIRDVPYKP+E++V TRKCE DA   +
Sbjct: 74  EVEDNSSSNMQTVRIPPVITVNKSAAYALYDLTYIRDVPYKPQEYHVTTRKCEHDAGPDI 133

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDE G+++  TQPICCPCG +RRMP+SCG+ FDKMIKGKANTAHCLRFPGDWF
Sbjct: 134 VQICERLRDEKGNVLEQTQPICCPCGPQRRMPSSCGDIFDKMIKGKANTAHCLRFPGDWF 193

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVF IGQ +LGFSV++ +K+G++VSEV +GPENRT  +NDNFL+ NLIGD  GYT+IPSF
Sbjct: 194 HVFGIGQRSLGFSVRVELKTGTRVSEVIIGPENRTATANDNFLKVNLIGDFGGYTSIPSF 253

Query: 181 EDFYLVIPRQGG-PGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCT 240
           EDFYLVIPR+    GQP +LG N+SMWMLLERVRFTLDGLECNKIGVGYE FN QP+FC+
Sbjct: 254 EDFYLVIPREAAEAGQPGSLGANYSMWMLLERVRFTLDGLECNKIGVGYEAFNTQPNFCS 313

Query: 241 SPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNT 300
           SP+WSCLHNQLWNFRE+D++RI R QLPLYG+EGRFERINQHPNAG HSFSIGVTE LNT
Sbjct: 314 SPYWSCLHNQLWNFRESDINRIDRHQLPLYGLEGRFERINQHPNAGPHSFSIGVTETLNT 373

Query: 301 NLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCS 360
           NL+IELRADD+EYV+QRSPGKI++I+IPTFEALTQFGVA V  KNTGEVEASYSLTF CS
Sbjct: 374 NLMIELRADDIEYVFQRSPGKIINIAIPTFEALTQFGVAAVIIKNTGEVEASYSLTFDCS 433

Query: 361 KEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVC 397
           K V+ +EEQ++I+KP  + +RSFKLYPT DQAAKY+C
Sbjct: 434 KGVAFVEEQFFIIKPKAVTTRSFKLYPTKDQAAKYIC 470

BLAST of Cucsat.G21374 vs. ExPASy Swiss-Prot
Match: Q5W6B9 (Protein HAPLESS 2-A OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2A PE=2 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 1.0e-151
Identity = 253/398 (63.57%), Postives = 311/398 (78.14%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYI-RDVPYKPEEFYVPTRKCEPDASAR 60
           E E ++ +  +++R PPV+TVSKS  Y LY LTY+ RDV Y+P+E YV T KCEP A A+
Sbjct: 77  EEENDTPSATKSIRDPPVITVSKSATYALYALTYLDRDVAYRPDEKYVKTHKCEPYAGAK 136

Query: 61  VVQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDW 120
           VV  CERL DE G++I  T+PICCPCG   R+ + CG+ + K+ KGKANTAHC+RFPGDW
Sbjct: 137 VVGECERLWDEKGNVIKQTEPICCPCG-PHRVQSKCGDIWSKLTKGKANTAHCVRFPGDW 196

Query: 121 FHVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPS 180
           FHVF IG W+L FS+++ VK GS V +V VGPEN+TVVS DNFLR  ++GD  GYT+IPS
Sbjct: 197 FHVFGIGAWSLRFSIRVQVKKGSSVWDVVVGPENKTVVSGDNFLRVKVVGDYTGYTSIPS 256

Query: 181 FEDFYLVIPRQG-GPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFC 240
           FED YLV PR+G G  QPQ+LG   S WM+L+RVRFTLDGLEC+KIGVGYE +  QP+FC
Sbjct: 257 FEDNYLVTPRKGTGSSQPQDLGNEHSKWMILDRVRFTLDGLECDKIGVGYEAYRNQPNFC 316

Query: 241 TSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLN 300
           ++P+ SCL NQLWNF E D  RI   QLPLY VEGRF+RINQHPNAG H+FS+GVTE LN
Sbjct: 317 SAPYGSCLGNQLWNFWEYDKRRIDNSQLPLYIVEGRFQRINQHPNAGAHTFSVGVTEDLN 376

Query: 301 TNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTC 360
           TNL+IEL ADD+EYVYQRSP KI+ I +PTFEAL+Q G+A V TKN G++E+SYSLTF C
Sbjct: 377 TNLLIELMADDIEYVYQRSPAKIIDIRVPTFEALSQVGIANVTTKNIGKLESSYSLTFKC 436

Query: 361 SKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVC 397
           S  +S +EEQ Y MKP+E+ +RSF+L  TTDQAA + C
Sbjct: 437 SSGISPVEEQLYTMKPDEVIARSFELRSTTDQAAMHQC 473

BLAST of Cucsat.G21374 vs. ExPASy Swiss-Prot
Match: B9G4M9 (Protein HAPLESS 2-B OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2B PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 3.1e-148
Identity = 253/398 (63.57%), Postives = 307/398 (77.14%), Query Frame = 0

Query: 1   EVEENSTNKMQ-TLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASAR 60
           EVEEN T   +  +R P ++T++KS  Y LY+LTY+RDV YKPEE +V TRKCEP+A A 
Sbjct: 82  EVEENGTEAGEMPIRDPLIITINKSEVYALYDLTYLRDVAYKPEEKFVKTRKCEPEAGAN 141

Query: 61  VVQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDW 120
           VV+ CERLRDE G II  T+P+CCPCG  RR+P+SCGN  DK+ KGKANTAHCLRFP DW
Sbjct: 142 VVKSCERLRDEKGSIIEHTEPVCCPCGPHRRVPSSCGNILDKVAKGKANTAHCLRFPDDW 201

Query: 121 FHVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPS 180
           FHVF IG+ +L FS+++ VK GS  SEV VGPENRTVVS D+ LR NL+GD  GYT++PS
Sbjct: 202 FHVFDIGRRSLWFSIRVQVKKGSSESEVIVGPENRTVVSEDSSLRVNLVGDFAGYTSLPS 261

Query: 181 FEDFYLVIPRQG-GPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFC 240
            E+FYLV PR+G G GQ + LG +FS WMLLERV FTLDGLECNKIGVGYE F  QP+FC
Sbjct: 262 LENFYLVTPRKGVGGGQLEVLGDDFSRWMLLERVLFTLDGLECNKIGVGYEAFRSQPNFC 321

Query: 241 TSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLN 300
           +SP  SCL +QL  F E D +R+   Q P Y V G+FERINQ+PNAG H+FS+G+ EVLN
Sbjct: 322 SSPLDSCLGDQLSKFWEIDKNRVNNSQPPQYVVLGKFERINQYPNAGVHTFSVGIPEVLN 381

Query: 301 TNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTC 360
           TNL+IEL ADD+EYVYQRS GKI+SI+I +FEAL+Q G A V TKN G +EASYSLTF C
Sbjct: 382 TNLMIELSADDIEYVYQRSSGKIISINISSFEALSQVGSARVKTKNIGRLEASYSLTFDC 441

Query: 361 SKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVC 397
              ++ +EEQY+IMKP+E   R+F L  +TDQA+ Y C
Sbjct: 442 LSGINPVEEQYFIMKPDEKLIRTFDLRSSTDQASNYTC 479

BLAST of Cucsat.G21374 vs. ExPASy Swiss-Prot
Match: A0A060A682 (Hapless 2 OS=Tetrahymena thermophila OX=5911 GN=HAP2 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.5e-38
Identity = 112/407 (27.52%), Postives = 181/407 (44.47%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTR--KCEPDASA 60
           ++ ++  NK   L+   +  V+KSP   L+ L Y++D   +P E  + T    C+ D   
Sbjct: 67  QLSDSGVNKQ--LQNSFIFEVTKSPVTALFPLIYLQDFNSQPLEQVIATTLFSCK-DGFY 126

Query: 61  RVVQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDK-------MIKGKANTAH 120
                C+   D  G  IL +Q  CC C     +    GN   +        +   + TAH
Sbjct: 127 DSSPTCKFQYDSKGQKILDSQGYCCYCSLSDIL--GMGNDLSRGKVCYALNLGAGSATAH 186

Query: 121 CLRFPGDWFHVFSIGQWTLGFSVQIHVKSGSKVSE------VSVGPENRTVVSNDNFLRA 180
           CL+F   W+  F I Q+ L F V I++ +    ++      + +   N T+ S+DN   +
Sbjct: 187 CLKFSPLWYSAFKIQQYQLYFEVNINIYTVDSQNQKNLKQTLKLSTSNPTMKSSDNSTIS 246

Query: 181 NLIGDLVGYTNIPSFEDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIG 240
            +IG             +YLV P    P     +    S WM +++  FTLDG +CNKIG
Sbjct: 247 KIIGTFTPTQPPADLSSYYLVKP--SFPATDPRVLQGISSWMFVDKTMFTLDGTQCNKIG 306

Query: 241 VGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAG 300
           V Y  F  Q   C+ P  SCL NQL N  ++DL  + + + P Y +E +    NQ    G
Sbjct: 307 VSYSGFRQQSSSCSQPVGSCLQNQLENLYQSDLILLSQNKQPKYLLESQ-GNFNQVQFQG 366

Query: 301 THSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNT 360
                 G++   +T + IE+ A  +++V     G I   SI  FE+ +  G      +N 
Sbjct: 367 QTILQQGLSGSASTLITIEIDAAQIKFVTNLGIGCISQCSINNFESHSGNGKLVALVQNQ 426

Query: 361 GEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAA 393
           G   A + L F CS  V  ++ Q   +  N++ + +  +   +D +A
Sbjct: 427 GNYSAEFVLGFNCSSNVQPIQGQKLFLTANQLYNFNCSVSVNSDISA 465

BLAST of Cucsat.G21374 vs. ExPASy Swiss-Prot
Match: A4GRC6 (Hapless 2 OS=Chlamydomonas reinhardtii OX=3055 GN=HAP2 PE=1 SV=2)

HSP 1 Score: 154.5 bits (389), Expect = 3.2e-36
Identity = 127/469 (27.08%), Postives = 195/469 (41.58%), Query Frame = 0

Query: 13  LRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYV-PTRKCEPDASARVVQICERLRDES 72
           L  P  ++++KSP +  Y L Y+    +KP E  + P+ K   D        C     + 
Sbjct: 95  LAAPLRVSLTKSPLWASYPLQYLSSFNWKPLEVILRPSNKVCKDGDWEDSPTCGWF-SQG 154

Query: 73  GHIILSTQPICCPCGAKRRMPTSCGNF----------------FDKMIKGKANTAHCLRF 132
           G  +  +Q  CC C + +    + G+                  D +I  K  +AHCL F
Sbjct: 155 GVRVADSQGFCCECSSSQVWDDTFGSSKERTRANLDCDFWSDPLDILIGRKPVSAHCLTF 214

Query: 133 PGDWFHVFSIGQWTLGFSVQIHVKSGSKVSEVS--------------------------- 192
              W+  + +G  +L F + I V+  +  S  +                           
Sbjct: 215 DPQWYSGYELGAASLQFEIAITVEVPTAPSPTTATTSATPRTNNSSSANSTNSTNSPAPQ 274

Query: 193 --------------VGPENRTVVSNDNFLRANLIGDLVGYTNIPSFEDFYLVIPR----Q 252
                         +GP      S    L A L+GDL  YT +P+  +  L++P+     
Sbjct: 275 FLSPPAPSTREVLHLGPSVPLASSASRLLSAKLLGDLAMYTQLPAISNQVLMVPQPPAAA 334

Query: 253 GGPGQP--QNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHN 312
              G P    L TN S WMLL++   ++DGL C+K+G G+  F  QP  C     +CL  
Sbjct: 335 AATGSPLDATLATNRSAWMLLDKTMLSMDGLACDKVGTGFSAFRYQPSGCGRAPQACLSG 394

Query: 313 QLWNFREADLSRIGRKQLPLYGV---EGRFERINQHPNAGTHSFSIGVTEVLNTNLVIEL 372
           QL +  EADL+RI   ++PLY +    G  +   Q  + G  SF++ VT    + + + +
Sbjct: 395 QLKDLWEADLARIADGRVPLYMITRFTGGSDTTLQSFSGGPLSFALPVTSHSQSLVTLSV 454

Query: 373 RADDVEYVYQRSPGKIMSISI--------PTFEALTQFGVATVATKNTGEVEASYSLTFT 402
            AD V  V  RSPGKI   ++          FEA+   G   V   NTG +++ Y+LT +
Sbjct: 455 AADGVRLVTNRSPGKITGAAVCRFAGTSCGGFEAVAARGYIYVNITNTGRLDSDYTLTVS 514

BLAST of Cucsat.G21374 vs. NCBI nr
Match: XP_008437220.1 (PREDICTED: protein HAPLESS 2 isoform X3 [Cucumis melo])

HSP 1 Score: 943 bits (2438), Expect = 0.0
Identity = 471/485 (97.11%), Postives = 473/485 (97.53%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAVQPYLRMLILVKLIELNANLLLL 420
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAVQPYLRMLILVKLIELNANLLLL
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAVQPYLRMLILVKLIELNANLLLL 491

Query: 421 LLSLTMDHRLLLLNSPRKRKMVSSIQSSSPGSSFGEASLTLSLGNLAEKCALDFLTSAVT 480
           LLSL MD RLLL N PRKRKMVSSIQSSSPGSSFGEAS TLSLGNLAEK ALDF TSAVT
Sbjct: 492 LLSLRMDQRLLLFNCPRKRKMVSSIQSSSPGSSFGEASSTLSLGNLAEKSALDFSTSAVT 551

Query: 481 YNIYV 485
           YNIYV
Sbjct: 552 YNIYV 556

BLAST of Cucsat.G21374 vs. NCBI nr
Match: XP_004143990.1 (protein HAPLESS 2 [Cucumis sativus])

HSP 1 Score: 823 bits (2126), Expect = 1.79e-295
Identity = 397/397 (100.00%), Postives = 397/397 (100.00%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 397
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 468

BLAST of Cucsat.G21374 vs. NCBI nr
Match: XP_008437218.1 (PREDICTED: protein HAPLESS 2 isoform X1 [Cucumis melo])

HSP 1 Score: 810 bits (2091), Expect = 3.25e-290
Identity = 390/397 (98.24%), Postives = 392/397 (98.74%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 397
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 468

BLAST of Cucsat.G21374 vs. NCBI nr
Match: KAA0042793.1 (protein HAPLESS 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 810 bits (2091), Expect = 1.02e-289
Identity = 390/397 (98.24%), Postives = 392/397 (98.74%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 397
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 468

BLAST of Cucsat.G21374 vs. NCBI nr
Match: KAG6579481.1 (Protein HAPLESS 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 799 bits (2063), Expect = 4.57e-286
Identity = 405/485 (83.51%), Postives = 428/485 (88.25%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKS AYVLY+LTYIRDVPYKP+EFYV TRKCEPDA+ARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSAAYVLYDLTYIRDVPYKPQEFYVRTRKCEPDATARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           V ICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VHICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQW+LGFS+QIHVKSGSK SEVSVGPENRTVVSND+FLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWSLGFSLQIHVKSGSKASEVSVGPENRTVVSNDSFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQN+G NFSMWMLLERVRFTLDGLECNKIGVGYETFN QPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNMGHNFSMWMLLERVRFTLDGLECNKIGVGYETFNSQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREA---------DLSRIGRKQLPLYGVEGRFERI---------NQHP 300
           PFWSCLHNQLWNFRE          +++ I   +     + GR +           +   
Sbjct: 312 PFWSCLHNQLWNFREVSIYNRQGIINITNIDMIRCIFSNLTGRSKSNWSKSVAIIWSGRK 371

Query: 301 NAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVAT 360
           NAGTHSFSIG+TEVLNTNLVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATV T
Sbjct: 372 NAGTHSFSIGITEVLNTNLVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVTT 431

Query: 361 KNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAVQPYL 420
           KNTGE+EASYSLTFTCSKEVSLMEEQY++MKP E+ASRSFKLYPTTDQAAKYVC+VQPYL
Sbjct: 432 KNTGELEASYSLTFTCSKEVSLMEEQYFLMKPKEVASRSFKLYPTTDQAAKYVCSVQPYL 491

Query: 421 RMLILVKLIELNANLLLLLLSLTMDHRLLLLNSPRKRKMVSSIQSSSPGSSFGEASLTLS 467
           RMLILVKL EL+ NLLLLLL LTMDHRL LLNSP KRKMVSSIQSSS G  +GE S TLS
Sbjct: 492 RMLILVKLTELSVNLLLLLLFLTMDHRLPLLNSPTKRKMVSSIQSSSLGRGYGETSSTLS 551

BLAST of Cucsat.G21374 vs. ExPASy TrEMBL
Match: A0A1S3AU32 (protein HAPLESS 2 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1)

HSP 1 Score: 943 bits (2438), Expect = 0.0
Identity = 471/485 (97.11%), Postives = 473/485 (97.53%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAVQPYLRMLILVKLIELNANLLLL 420
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAVQPYLRMLILVKLIELNANLLLL
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAVQPYLRMLILVKLIELNANLLLL 491

Query: 421 LLSLTMDHRLLLLNSPRKRKMVSSIQSSSPGSSFGEASLTLSLGNLAEKCALDFLTSAVT 480
           LLSL MD RLLL N PRKRKMVSSIQSSSPGSSFGEAS TLSLGNLAEK ALDF TSAVT
Sbjct: 492 LLSLRMDQRLLLFNCPRKRKMVSSIQSSSPGSSFGEASSTLSLGNLAEKSALDFSTSAVT 551

Query: 481 YNIYV 485
           YNIYV
Sbjct: 552 YNIYV 556

BLAST of Cucsat.G21374 vs. ExPASy TrEMBL
Match: A0A1S3ATM0 (protein HAPLESS 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1)

HSP 1 Score: 810 bits (2091), Expect = 1.57e-290
Identity = 390/397 (98.24%), Postives = 392/397 (98.74%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 397
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 468

BLAST of Cucsat.G21374 vs. ExPASy TrEMBL
Match: A0A5A7THM3 (Protein HAPLESS 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002960 PE=3 SV=1)

HSP 1 Score: 810 bits (2091), Expect = 4.96e-290
Identity = 390/397 (98.24%), Postives = 392/397 (98.74%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 397
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 468

BLAST of Cucsat.G21374 vs. ExPASy TrEMBL
Match: A0A0A0KQY8 (HAP2-GCS1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155520 PE=3 SV=1)

HSP 1 Score: 801 bits (2070), Expect = 2.37e-287
Identity = 391/397 (98.49%), Postives = 391/397 (98.49%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGD  
Sbjct: 132 VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGD-- 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
               IGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF
Sbjct: 192 ---CIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 397
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 463

BLAST of Cucsat.G21374 vs. ExPASy TrEMBL
Match: A0A6J1I226 (protein HAPLESS 2 OS=Cucurbita maxima OX=3661 GN=LOC111468909 PE=3 SV=1)

HSP 1 Score: 788 bits (2036), Expect = 4.66e-282
Identity = 375/397 (94.46%), Postives = 386/397 (97.23%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKS AYVLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSAAYVLYELTYIRDVPYKPEEFYVRTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           V ICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VHICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQW+LGFSVQIHVKSGSK SEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWSLGFSVQIHVKSGSKASEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQN+G NFSMWMLLERVRFTLDGLECNKIGVGYETFN QPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNMGHNFSMWMLLERVRFTLDGLECNKIGVGYETFNSQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR Q+PLYGVEGRFERINQHPNAGTHSFSIG+TEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQMPLYGVEGRFERINQHPNAGTHSFSIGITEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVAT+ TKNTGE+EASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATITTKNTGELEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCA 397
           EVSLMEEQY++MKP E+ASRSFKLYPTTDQAAKYVC+
Sbjct: 432 EVSLMEEQYFLMKPKEVASRSFKLYPTTDQAAKYVCS 468

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JP362.0e-18775.57Protein HAPLESS 2 OS=Arabidopsis thaliana OX=3702 GN=HAP2 PE=1 SV=1[more]
Q5W6B91.0e-15163.57Protein HAPLESS 2-A OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2A PE=2 SV=1[more]
B9G4M93.1e-14863.57Protein HAPLESS 2-B OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2B PE=3 SV=1[more]
A0A060A6827.5e-3827.52Hapless 2 OS=Tetrahymena thermophila OX=5911 GN=HAP2 PE=1 SV=1[more]
A4GRC63.2e-3627.08Hapless 2 OS=Chlamydomonas reinhardtii OX=3055 GN=HAP2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_008437220.10.097.11PREDICTED: protein HAPLESS 2 isoform X3 [Cucumis melo][more]
XP_004143990.11.79e-295100.00protein HAPLESS 2 [Cucumis sativus][more]
XP_008437218.13.25e-29098.24PREDICTED: protein HAPLESS 2 isoform X1 [Cucumis melo][more]
KAA0042793.11.02e-28998.24protein HAPLESS 2 isoform X1 [Cucumis melo var. makuwa][more]
KAG6579481.14.57e-28683.51Protein HAPLESS 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A1S3AU320.097.11protein HAPLESS 2 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1[more]
A0A1S3ATM01.57e-29098.24protein HAPLESS 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1[more]
A0A5A7THM34.96e-29098.24Protein HAPLESS 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A0A0KQY82.37e-28798.49HAP2-GCS1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155520 P... [more]
A0A6J1I2264.66e-28294.46protein HAPLESS 2 OS=Cucurbita maxima OX=3661 GN=LOC111468909 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018928Generative cell specific-1/HAP2 domainPFAMPF10699HAP2-GCS1coord: 2..483
e-value: 2.3E-120
score: 402.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 544..569
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 544..575
IPR040326HAP2/GCS1PANTHERPTHR31764PROTEIN HAPLESS 2coord: 1..589

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G21374.T6Cucsat.G21374.T6mRNA
Cucsat.G21374.T1Cucsat.G21374.T1mRNA
Cucsat.G21374.T4Cucsat.G21374.T4mRNA
Cucsat.G21374.T2Cucsat.G21374.T2mRNA
Cucsat.G21374.T3Cucsat.G21374.T3mRNA
Cucsat.G21374.T5Cucsat.G21374.T5mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061936 fusion of sperm to egg plasma membrane involved in double fertilization forming a zygote and endosperm
biological_process GO:0048235 pollen sperm cell differentiation
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0005886 plasma membrane