Cucsat.G21374.T5 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G21374.T5
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionprotein HAPLESS 2
Locationctg910: 1990756 .. 1993113 (+)
RNA-Seq ExpressionCucsat.G21374.T5
SyntenyCucsat.G21374.T5
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGCTTATTCCACCACAAAAGCATTTGATGGAACATTTTGTAATCGAAGATTTTTAGACATTAATGCGTACAACGGAAAGGCTACCTTGATATATATAACTTTGCTTGTTTGTTCTTATCTTCTGTTGCTAAGCTTAAAATATTCAATTGTGATGGAAGGGTGGTCCTGGTCAACCACAGAATTTAGGTACCAATTTCTCTATGTGGATGCTACTAGAAAGAGTGAGATTTACTTTAGATGGTCTTGAATGCAACAAAATTGGTGTTGGTTATGAGACTTTTAATGGACAGCCTGATTTCTGTACATCCCCATTTTGGAGTTGTTTGCACAACCAATTATGGAATTTCAGGGAGGTAAGCTTTTAATCAAGAAAGGGTACTACTAACATTGATTGCTTTTATATGATCTGATACGTTTTCCCAATTTTCGAAAGGCCGATTTGAGTCGAATTGGTAGGAAACAATTGCCATTATATGGAGTGGAAGGACGGTTTGAGAGGATTAATCAACATCCAGTTAAGTATTGGAGGATATAGAATTATTCTTCATTCATGAGTATTCATATTGCTAAACGTTTTGTGTTGGGTGGGTTGAAGAATGCTGGGACACATTCGTTCTCCATTGGAGTCACTGAAGTTCTCAATACAAATCTTGTAATAGAATTGCGTGCTGATGATGTTGAGTATGTTTACCAAAGGTGAGTTGTTAGCACTTCTTGTAATTAAACTGCATCCCGAATGTGACTTGGTATGAATACGCTACGATACATGTTTAAGTGTCATTGTGCTAACAAGTGACCAATATATGTCCAACAAGCGTCGAAGTGTCTAAGTGCTTGCCAAACTTAAGTGTCCGTGTTTTGAACCAAATATATATATTTTTTTGAAGATATGAAAAGCGATTAGCAATCACATATGTTCTTCAATAGGCTACATTTTATTTAATGTAGGAGTCCTGGGAAAATTATGAGCATCAGCATCCCAACTTTTGAAGCCCTCACACAATTTGGAGTTGCTACAGTTGCAACTAAGAACACTGGAGAAGTGGAAGCATCTTATAGCTTAACGGTATTTACTAAGCTTTTACTACAATGAGATATATGGTTGGAATACTTGAGTGAAGCTTTTATTGTTTCTTTTGATCTCCTGCACATTATTTCAACTGGATGGATTTTTCTTTCTTGCAGTTTACTTGTTCAAAAGAAGTCAGTCTCATGGAGGTACCGTCTTTCGTGGAGACATTTTGATAGAATTTTGTTCATGTGATGAGTTTTTGATGAAAATCTAGATGATTTCAGGAACAATATTATATCATGAAGCCAAATGAAATTGCTAGCCGTTCATTTAAACTCTACCCAACTACTGATCAAGCAGCAAAATATGTTTGTGCTGGTAAGATGAGTAACTAATTTGTGCTTCTTTTCTCATTAAATTTATTTAGTTCAGATTCAAGATTCTTTGAGACAAAACTTCTCTTTGTTGATAGTGCAGCCATACTTAAGGATGCTGATTTTAGTGAAGTTGATAGAGCTGAATGCCAATTTGCTACTACTGCTACTGTCCTTGACAATGGATCACAGGTTCCTTTTACAGTTCTAGAATCATGGTGAATCAAGTATCCTTCTTCCATTAGTGAGTTTGGCTTTTTTTTTTTTTTTTTAACAGATTACTCCTTTTGAACTCCCCAAGAAAAAGGAAAATGGTTTCATCCATTCAATCAAGCTCGCCTGGAAGCAGTTTTGGGGAAGCGTCATTGACTTTGTCACTGGGAAATCTTGCAGGTATTTGCTATCAAACTCTCATAAACCATTTACCTTCCATTGACATACCATCCCCAATGACACGTTTGCCTCTTTTGAACAGAAAAGTGTGCTCTGGATTTTTTGACTTCAGCTGTCACATACAATATATATGTTTGAGTTGGCTTGTGTTGTTTGGTCTCTTTTTGGCCACTTTCCCCGCAGGTAACTATTATCCAACACTTGAAAAGTGATTTCAAACAAGTCGGCCTAAAATTAAAAATAAGCATCTCATCTTCATAATTTGTTTCAATTACTATGCAGTACTTGTGATACTGTGGGTTTTACATCAGAAAGGCTTATTTGACCCTCTTTATGACTGGTGGGAGGATATGTTTTGTCACAAAAGTGAGCCCACAAGGTCCACTTGGAAGTATAGAGGTGAAAGAAAACATTACCATAGACATGGTAGTAGGCATCACCAAAATCATGGGAGTGGATACAAGAGAAGAAGCCATGAATTACACAAAAAGCACAAACATTCTGAGAGAGACACAGATTACTTTCTTCACCATGTGCACAGGAAAAAAGGTAAGAGGGGACACAATAGAGTGTAGAA

Coding sequence (CDS)

ATGTGGATGCTACTAGAAAGAGTGAGATTTACTTTAGATGGTCTTGAATGCAACAAAATTGGTGTTGGTTATGAGACTTTTAATGGACAGCCTGATTTCTGTACATCCCCATTTTGGAGTTGTTTGCACAACCAATTATGGAATTTCAGGGAGGCCGATTTGAGTCGAATTGGTAGGAAACAATTGCCATTATATGGAGTGGAAGGACGGTTTGAGAGGATTAATCAACATCCAAATGCTGGGACACATTCGTTCTCCATTGGAGTCACTGAAGTTCTCAATACAAATCTTGTAATAGAATTGCGTGCTGATGATGTTGAGTATGTTTACCAAAGGAGTCCTGGGAAAATTATGAGCATCAGCATCCCAACTTTTGAAGCCCTCACACAATTTGGAGTTGCTACAGTTGCAACTAAGAACACTGGAGAAGTGGAAGCATCTTATAGCTTAACGTTTACTTGTTCAAAAGAAGTCAGTCTCATGGAGGAACAATATTATATCATGAAGCCAAATGAAATTGCTAGCCGTTCATTTAAACTCTACCCAACTACTGATCAAGCAGCAAAATATGTTTGTGCTGCCATACTTAAGGATGCTGATTTTAGTGAAGTTGATAGAGCTGAATGCCAATTTGCTACTACTGCTACTGTCCTTGACAATGGATCACAGATTACTCCTTTTGAACTCCCCAAGAAAAAGGAAAATGGTTTCATCCATTCAATCAAGCTCGCCTGGAAGCAGTTTTGGGGAAGCGTCATTGACTTTGTCACTGGGAAATCTTGCAGAAAAGTGTGCTCTGGATTTTTTGACTTCAGCTGTCACATACAATATATATGTTTGAGTTGGCTTGTGTTGTTTGGTCTCTTTTTGGCCACTTTCCCCGCAGTACTTGTGATACTGTGGGTTTTACATCAGAAAGGCTTATTTGACCCTCTTTATGACTGGTGGGAGGATATGTTTTGTCACAAAAGTGAGCCCACAAGGTCCACTTGGAAGTATAGAGGTGAAAGAAAACATTACCATAGACATGGTAGTAGGCATCACCAAAATCATGGGAGTGGATACAAGAGAAGAAGCCATGAATTACACAAAAAGCACAAACATTCTGAGAGAGACACAGATTACTTTCTTCACCATGTGCACAGGAAAAAAGGTAAGAGGGGACACAATAGAGTGTAG

Protein sequence

MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV
Homology
BLAST of Cucsat.G21374.T5 vs. ExPASy Swiss-Prot
Match: F4JP36 (Protein HAPLESS 2 OS=Arabidopsis thaliana OX=3702 GN=HAP2 PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 7.7e-150
Identity = 259/407 (63.64%), Postives = 311/407 (76.41%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYE FN QP+FC+SP+WSCLHNQLWNFRE+D++RI R 
Sbjct: 279 MWMLLERVRFTLDGLECNKIGVGYEAFNTQPNFCSSPYWSCLHNQLWNFRESDINRIDRH 338

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYG+EGRFERINQHPNAG HSFSIGVTE LNTNL+IELRADD+EYV+QRSPGKI++I
Sbjct: 339 QLPLYGLEGRFERINQHPNAGPHSFSIGVTETLNTNLMIELRADDIEYVFQRSPGKIINI 398

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVA V  KNTGEVEASYSLTF CSK V+ +EEQ++I+KP  + +RSFKL
Sbjct: 399 AIPTFEALTQFGVAAVIIKNTGEVEASYSLTFDCSKGVAFVEEQFFIIKPKAVTTRSFKL 458

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQIT-PFELPKKKENGFIH 240
           YPT DQAAKY+C AILKD+ FSEVDRAECQF+TTATVLDNG+Q+T PF++P+ +  GF  
Sbjct: 459 YPTKDQAAKYICTAILKDSQFSEVDRAECQFSTTATVLDNGTQVTNPFQIPETQPKGFFD 518

Query: 241 SIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVI 300
           SI++ W +    ++DF+TG +CR  CS FFDFSCHIQY+CLSW+V+FGL LA FP   ++
Sbjct: 519 SIRILWTKIINGLVDFITGDTCRNKCSSFFDFSCHIQYVCLSWMVMFGLLLALFPITCLL 578

Query: 301 LWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRS 360
           LW+LHQKGLFDP YDWWED F           +     +H+H H  RHH NH     RR+
Sbjct: 579 LWLLHQKGLFDPCYDWWEDHFDLDHHRRLLPSRADVVNRHHHHHKHRHHHNH----HRRT 638

Query: 361 HELHKKH---------------KHSERDTDYFLHHVHRKKGKRGHNR 392
           H+ HK H                HS+    + LH VH+   ++   R
Sbjct: 639 HQRHKHHHGQDDDVLQKMMLERDHSDSHYYHQLHRVHKDSKQKQRRR 681

BLAST of Cucsat.G21374.T5 vs. ExPASy Swiss-Prot
Match: Q5W6B9 (Protein HAPLESS 2-A OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2A PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 9.8e-113
Identity = 217/401 (54.11%), Postives = 268/401 (66.83%), Query Frame = 0

Query: 2   WMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQ 61
           WM+L+RVRFTLDGLEC+KIGVGYE +  QP+FC++P+ SCL NQLWNF E D  RI   Q
Sbjct: 283 WMILDRVRFTLDGLECDKIGVGYEAYRNQPNFCSAPYGSCLGNQLWNFWEYDKRRIDNSQ 342

Query: 62  LPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSIS 121
           LPLY VEGRF+RINQHPNAG H+FS+GVTE LNTNL+IEL ADD+EYVYQRSP KI+ I 
Sbjct: 343 LPLYIVEGRFQRINQHPNAGAHTFSVGVTEDLNTNLLIELMADDIEYVYQRSPAKIIDIR 402

Query: 122 IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLY 181
           +PTFEAL+Q G+A V TKN G++E+SYSLTF CS  +S +EEQ Y MKP+E+ +RSF+L 
Sbjct: 403 VPTFEALSQVGIANVTTKNIGKLESSYSLTFKCSSGISPVEEQLYTMKPDEVIARSFELR 462

Query: 182 PTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHSI 241
            TTDQAA + C AILK +DFSE+DR   +F+T ATV +NG+QI P      K+ GF  SI
Sbjct: 463 STTDQAAMHQCEAILKASDFSELDREGYRFSTAATVYNNGAQIGPTN--DHKKGGFWDSI 522

Query: 242 KLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVILW 301
           K  W+    ++IDF+TG+ C   C   FDF CHIQY+C+ W++L    L   PA +V LW
Sbjct: 523 KALWR----NLIDFLTGRLCWTKCPRLFDFGCHIQYVCIGWILL----LLLIPAAVVFLW 582

Query: 302 VLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHY-------HRHGSRHHQNHGSG 361
           +LHQ+GLFDPLYDWW           R   K R  R H+       H HG  HH  HG  
Sbjct: 583 LLHQEGLFDPLYDWWGLEPDDDYRARRRHQKGRHHRHHHDHRHRHGHSHGDHHHHYHGGH 642

Query: 362 YKRRSHELHKKHKHSERDTDYFLHH----VHRKKGKRGHNR 392
           ++RR H       H     D   HH     H  +  R H+R
Sbjct: 643 HQRRRH-------HHPPAWDVEGHHHDRQQHSHEAGRNHHR 666

BLAST of Cucsat.G21374.T5 vs. ExPASy Swiss-Prot
Match: B9G4M9 (Protein HAPLESS 2-B OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2B PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.0e-93
Identity = 198/378 (52.38%), Postives = 247/378 (65.34%), Query Frame = 0

Query: 2   WMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQ 61
           WMLLERV FTLDGLECNKIGVGYE F  QP+FC+SP  SCL +QL  F E D +R+   Q
Sbjct: 289 WMLLERVLFTLDGLECNKIGVGYEAFRSQPNFCSSPLDSCLGDQLSKFWEIDKNRVNNSQ 348

Query: 62  LPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSIS 121
            P Y V G+FERINQ+PNAG H+FS+G+ EVLNTNL+IEL ADD+EYVYQRS GKI+SI+
Sbjct: 349 PPQYVVLGKFERINQYPNAGVHTFSVGIPEVLNTNLMIELSADDIEYVYQRSSGKIISIN 408

Query: 122 IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLY 181
           I +FEAL+Q G A V TKN G +EASYSLTF C   ++ +EEQY+IMKP+E   R+F L 
Sbjct: 409 ISSFEALSQVGSARVKTKNIGRLEASYSLTFDCLSGINPVEEQYFIMKPDEKLIRTFDLR 468

Query: 182 PTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKEN-GFIHS 241
            +TDQA+ Y C AILK +DFSE+DR E QF+TTATVL+NG+QI   E   K    GF  +
Sbjct: 469 SSTDQASNYTCQAILKASDFSELDRKESQFSTTATVLNNGTQIGSSENHTKGGIWGFFEA 528

Query: 242 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 301
           IK    + W  +I+F TG +C   C  F  F            V+ GL L      + +L
Sbjct: 529 IKAWCAKMWHMLINFFTGTTCSTRCWSFLKF------------VIHGLLL------VAVL 588

Query: 302 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 361
           W+LH+KGLFDPLY WW+ +   +++      + R +R H HRH S HH  H    KR   
Sbjct: 589 WLLHRKGLFDPLYYWWDGVVGSEAQERA---RRRHKRAHSHRH-SHHHDAH----KRHKT 640

Query: 362 EL--HKKHK--HSERDTD 375
           EL  H++H   H   D D
Sbjct: 649 ELAGHRRHHVLHIHDDDD 640

BLAST of Cucsat.G21374.T5 vs. ExPASy Swiss-Prot
Match: A4GRC6 (Hapless 2 OS=Chlamydomonas reinhardtii OX=3055 GN=HAP2 PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 4.7e-22
Identity = 77/233 (33.05%), Postives = 113/233 (48.50%), Query Frame = 0

Query: 2   WMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQ 61
           WMLL++   ++DGL C+K+G G+  F  QP  C     +CL  QL +  EADL+RI   +
Sbjct: 351 WMLLDKTMLSMDGLACDKVGTGFSAFRYQPSGCGRAPQACLSGQLKDLWEADLARIADGR 410

Query: 62  LPLYGV---EGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIM 121
           +PLY +    G  +   Q  + G  SF++ VT    + + + + AD V  V  RSPGKI 
Sbjct: 411 VPLYMITRFTGGSDTTLQSFSGGPLSFALPVTSHSQSLVTLSVAADGVRLVTNRSPGKIT 470

Query: 122 SISI--------PTFEALTQFGVATVATKNTGEVEASYSLTFT-CSKEVSLMEEQYYIMK 181
             ++          FEA+   G   V   NTG +++ Y+LT + CS  V  +E +   ++
Sbjct: 471 GAAVCRFAGTSCGGFEAVAARGYIYVNITNTGRLDSDYTLTVSNCSSNVRPIEARTLAVR 530

Query: 182 PNEIASRS--FKLYPTTDQ--AAKYVCAAILKDADFSEVDRAECQFATTATVL 219
               AS     +LY   DQ  AA   C   L D+  +  D     F T AT L
Sbjct: 531 AGSAASLDPPMELY-VEDQAAAAARTCTVSLYDSVGAVTDSLTLSFYTNATQL 582

BLAST of Cucsat.G21374.T5 vs. ExPASy Swiss-Prot
Match: A0A060A682 (Hapless 2 OS=Tetrahymena thermophila OX=5911 GN=HAP2 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.9e-19
Identity = 89/368 (24.18%), Postives = 155/368 (42.12%), Query Frame = 0

Query: 2   WMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQ 61
           WM +++  FTLDG +CNKIGV Y  F  Q   C+ P  SCL NQL N  ++DL  + + +
Sbjct: 280 WMFVDKTMFTLDGTQCNKIGVSYSGFRQQSSSCSQPVGSCLQNQLENLYQSDLILLSQNK 339

Query: 62  LPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSIS 121
            P Y +E +    NQ    G      G++   +T + IE+ A  +++V     G I   S
Sbjct: 340 QPKYLLESQ-GNFNQVQFQGQTILQQGLSGSASTLITIEIDAAQIKFVTNLGIGCISQCS 399

Query: 122 IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLY 181
           I  FE+ +  G      +N G   A + L F CS  V  ++ Q   +  N++ + +  + 
Sbjct: 400 INNFESHSGNGKLVALVQNQGNYSAEFVLGFNCSSNVQPIQGQKLFLTANQLYNFNCSVS 459

Query: 182 PTTD-QAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 241
             +D  A    C   L DA  +++D     F TT+T   +       +    +E      
Sbjct: 460 VNSDISAINNNCTINLYDAIGNQLDSKNILFNTTSTNHTSNQGNNTGQQQSSQE------ 519

Query: 242 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 301
                         + + +SC   CS F+ F C+    C+         +A   + L ++
Sbjct: 520 --------------YKSSQSCSDKCSSFWSFWCYFSAGCIKEAFKSIASIAGVASALALV 579

Query: 302 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 361
             L + G   P+  +     C   +      K + ++K      S + ++  S    +S+
Sbjct: 580 IFLAKNGYLVPIIRFLCCCCCKSKKKENEKNKDKTDKKSIQESCS-YDRSCCSHSISQSY 625

Query: 362 ELHKKHKH 369
           ++  K+K+
Sbjct: 640 QVENKNKY 625

BLAST of Cucsat.G21374.T5 vs. NCBI nr
Match: XP_004143990.1 (protein HAPLESS 2 [Cucumis sativus])

HSP 1 Score: 810 bits (2091), Expect = 9.04e-292
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL
Sbjct: 396 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS
Sbjct: 456 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 300
           IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL
Sbjct: 516 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 575

Query: 301 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 360
           WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH
Sbjct: 576 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 635

Query: 361 ELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 392
           ELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV
Sbjct: 636 ELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 667

BLAST of Cucsat.G21374.T5 vs. NCBI nr
Match: XP_008437218.1 (PREDICTED: protein HAPLESS 2 isoform X1 [Cucumis melo])

HSP 1 Score: 769 bits (1986), Expect = 7.34e-276
Identity = 374/387 (96.64%), Postives = 378/387 (97.67%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGR 
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRN 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL
Sbjct: 396 NIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVL+NGS+ITPF+LPKKKENGFI S
Sbjct: 456 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLENGSEITPFQLPKKKENGFIDS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 300
           IKLAWKQFWGSVIDFVTGKSCRK CSGFFDFSCHIQYICLSWLVLFGLFLATFP VLVIL
Sbjct: 516 IKLAWKQFWGSVIDFVTGKSCRKECSGFFDFSCHIQYICLSWLVLFGLFLATFPIVLVIL 575

Query: 301 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 360
           WVLHQKGLFDPLYDWWED FCHKSE TRSTWKYRGERKH HRHGSRHH NHGSGYKRRSH
Sbjct: 576 WVLHQKGLFDPLYDWWEDTFCHKSELTRSTWKYRGERKHNHRHGSRHHHNHGSGYKRRSH 635

Query: 361 ELHKKHKHSERDTDYFLHHVHRKKGKR 387
           ELHKKHKHSERDTDYFLHHVHRKK KR
Sbjct: 636 ELHKKHKHSERDTDYFLHHVHRKKVKR 662

BLAST of Cucsat.G21374.T5 vs. NCBI nr
Match: KAA0042793.1 (protein HAPLESS 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 767 bits (1981), Expect = 1.32e-274
Identity = 379/420 (90.24%), Postives = 384/420 (91.43%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGR 
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRN 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL
Sbjct: 396 NIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVL+NGS+ITPF+LPKKKENGFI S
Sbjct: 456 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLENGSEITPFQLPKKKENGFIDS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCR----------------------------KVCSGFFDFS 300
           IKLAWKQFWGSVIDFVTGKSCR                            K CSGFFDFS
Sbjct: 516 IKLAWKQFWGSVIDFVTGKSCRYLLSKTIYLPLTYRPLYFQMTCFSLLNRKECSGFFDFS 575

Query: 301 CHIQYICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWK 360
           CHIQYICLSWLVLFGLFLATFP VLVILWVLHQKGLFDPLYDWWED FCHKSE TRSTWK
Sbjct: 576 CHIQYICLSWLVLFGLFLATFPIVLVILWVLHQKGLFDPLYDWWEDTFCHKSELTRSTWK 635

Query: 361 YRGERKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 392
           YRGERKH HRHGSRHH NHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGK+GHNRV
Sbjct: 636 YRGERKHNHRHGSRHHHNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKKGHNRV 695

BLAST of Cucsat.G21374.T5 vs. NCBI nr
Match: XP_038875720.1 (protein HAPLESS 2 [Benincasa hispida])

HSP 1 Score: 750 bits (1937), Expect = 2.39e-268
Identity = 361/392 (92.09%), Postives = 378/392 (96.43%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFN QPDFC SPF SCLH+QLWN+READLSRIGR 
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNSQPDFCKSPFRSCLHDQLWNYREADLSRIGRN 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVATV TKNTGEVEASYSLTFTCSKEVSLMEEQY+IMKP E+AS SFKL
Sbjct: 396 NIPTFEALTQFGVATVITKNTGEVEASYSLTFTCSKEVSLMEEQYFIMKPKEVASLSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPF+LPKKKENGFI S
Sbjct: 456 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFQLPKKKENGFIDS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 300
           IKL WK+ W S+++FVTGKSCRK CSGFFDFSCHIQYICLSWLVLFGLFL TFPAVLVIL
Sbjct: 516 IKLIWKKLWESIVNFVTGKSCRKECSGFFDFSCHIQYICLSWLVLFGLFLTTFPAVLVIL 575

Query: 301 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 360
           WVLHQKGLFDPL+DWWED+FCHK+EPTRSTWK+RGERKH +RHGSRHHQNHGSGYKRRSH
Sbjct: 576 WVLHQKGLFDPLFDWWEDIFCHKNEPTRSTWKHRGERKHSYRHGSRHHQNHGSGYKRRSH 635

Query: 361 ELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 392
           ELHKKHKHS++DTDYFLHHVHRKKGKRGHNRV
Sbjct: 636 ELHKKHKHSDKDTDYFLHHVHRKKGKRGHNRV 667

BLAST of Cucsat.G21374.T5 vs. NCBI nr
Match: XP_008437219.1 (PREDICTED: protein HAPLESS 2 isoform X2 [Cucumis melo])

HSP 1 Score: 739 bits (1908), Expect = 3.60e-264
Identity = 363/387 (93.80%), Postives = 367/387 (94.83%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGR 
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRN 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVATVATKNTGEVEASYSLT           EQYYIMKPNEIASRSFKL
Sbjct: 396 NIPTFEALTQFGVATVATKNTGEVEASYSLT-----------EQYYIMKPNEIASRSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVL+NGS+ITPF+LPKKKENGFI S
Sbjct: 456 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLENGSEITPFQLPKKKENGFIDS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 300
           IKLAWKQFWGSVIDFVTGKSCRK CSGFFDFSCHIQYICLSWLVLFGLFLATFP VLVIL
Sbjct: 516 IKLAWKQFWGSVIDFVTGKSCRKECSGFFDFSCHIQYICLSWLVLFGLFLATFPIVLVIL 575

Query: 301 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 360
           WVLHQKGLFDPLYDWWED FCHKSE TRSTWKYRGERKH HRHGSRHH NHGSGYKRRSH
Sbjct: 576 WVLHQKGLFDPLYDWWEDTFCHKSELTRSTWKYRGERKHNHRHGSRHHHNHGSGYKRRSH 635

Query: 361 ELHKKHKHSERDTDYFLHHVHRKKGKR 387
           ELHKKHKHSERDTDYFLHHVHRKK KR
Sbjct: 636 ELHKKHKHSERDTDYFLHHVHRKKVKR 651

BLAST of Cucsat.G21374.T5 vs. ExPASy TrEMBL
Match: A0A0A0KQY8 (HAP2-GCS1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155520 PE=3 SV=1)

HSP 1 Score: 810 bits (2091), Expect = 3.66e-292
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK
Sbjct: 271 MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 330

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 331 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 390

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL
Sbjct: 391 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 450

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS
Sbjct: 451 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 510

Query: 241 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 300
           IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL
Sbjct: 511 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 570

Query: 301 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 360
           WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH
Sbjct: 571 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 630

Query: 361 ELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 392
           ELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV
Sbjct: 631 ELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 662

BLAST of Cucsat.G21374.T5 vs. ExPASy TrEMBL
Match: A0A1S3ATM0 (protein HAPLESS 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1)

HSP 1 Score: 769 bits (1986), Expect = 3.55e-276
Identity = 374/387 (96.64%), Postives = 378/387 (97.67%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGR 
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRN 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL
Sbjct: 396 NIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVL+NGS+ITPF+LPKKKENGFI S
Sbjct: 456 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLENGSEITPFQLPKKKENGFIDS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 300
           IKLAWKQFWGSVIDFVTGKSCRK CSGFFDFSCHIQYICLSWLVLFGLFLATFP VLVIL
Sbjct: 516 IKLAWKQFWGSVIDFVTGKSCRKECSGFFDFSCHIQYICLSWLVLFGLFLATFPIVLVIL 575

Query: 301 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 360
           WVLHQKGLFDPLYDWWED FCHKSE TRSTWKYRGERKH HRHGSRHH NHGSGYKRRSH
Sbjct: 576 WVLHQKGLFDPLYDWWEDTFCHKSELTRSTWKYRGERKHNHRHGSRHHHNHGSGYKRRSH 635

Query: 361 ELHKKHKHSERDTDYFLHHVHRKKGKR 387
           ELHKKHKHSERDTDYFLHHVHRKK KR
Sbjct: 636 ELHKKHKHSERDTDYFLHHVHRKKVKR 662

BLAST of Cucsat.G21374.T5 vs. ExPASy TrEMBL
Match: A0A5A7THM3 (Protein HAPLESS 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002960 PE=3 SV=1)

HSP 1 Score: 767 bits (1981), Expect = 6.37e-275
Identity = 379/420 (90.24%), Postives = 384/420 (91.43%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGR 
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRN 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL
Sbjct: 396 NIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVL+NGS+ITPF+LPKKKENGFI S
Sbjct: 456 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLENGSEITPFQLPKKKENGFIDS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCR----------------------------KVCSGFFDFS 300
           IKLAWKQFWGSVIDFVTGKSCR                            K CSGFFDFS
Sbjct: 516 IKLAWKQFWGSVIDFVTGKSCRYLLSKTIYLPLTYRPLYFQMTCFSLLNRKECSGFFDFS 575

Query: 301 CHIQYICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWK 360
           CHIQYICLSWLVLFGLFLATFP VLVILWVLHQKGLFDPLYDWWED FCHKSE TRSTWK
Sbjct: 576 CHIQYICLSWLVLFGLFLATFPIVLVILWVLHQKGLFDPLYDWWEDTFCHKSELTRSTWK 635

Query: 361 YRGERKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 392
           YRGERKH HRHGSRHH NHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGK+GHNRV
Sbjct: 636 YRGERKHNHRHGSRHHHNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKKGHNRV 695

BLAST of Cucsat.G21374.T5 vs. ExPASy TrEMBL
Match: A0A1S3ATH0 (protein HAPLESS 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1)

HSP 1 Score: 739 bits (1908), Expect = 1.74e-264
Identity = 363/387 (93.80%), Postives = 367/387 (94.83%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGR 
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRN 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVATVATKNTGEVEASYSLT           EQYYIMKPNEIASRSFKL
Sbjct: 396 NIPTFEALTQFGVATVATKNTGEVEASYSLT-----------EQYYIMKPNEIASRSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVL+NGS+ITPF+LPKKKENGFI S
Sbjct: 456 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLENGSEITPFQLPKKKENGFIDS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 300
           IKLAWKQFWGSVIDFVTGKSCRK CSGFFDFSCHIQYICLSWLVLFGLFLATFP VLVIL
Sbjct: 516 IKLAWKQFWGSVIDFVTGKSCRKECSGFFDFSCHIQYICLSWLVLFGLFLATFPIVLVIL 575

Query: 301 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSH 360
           WVLHQKGLFDPLYDWWED FCHKSE TRSTWKYRGERKH HRHGSRHH NHGSGYKRRSH
Sbjct: 576 WVLHQKGLFDPLYDWWEDTFCHKSELTRSTWKYRGERKHNHRHGSRHHHNHGSGYKRRSH 635

Query: 361 ELHKKHKHSERDTDYFLHHVHRKKGKR 387
           ELHKKHKHSERDTDYFLHHVHRKK KR
Sbjct: 636 ELHKKHKHSERDTDYFLHHVHRKKVKR 651

BLAST of Cucsat.G21374.T5 vs. ExPASy TrEMBL
Match: A0A6J1I226 (protein HAPLESS 2 OS=Cucurbita maxima OX=3661 GN=LOC111468909 PE=3 SV=1)

HSP 1 Score: 723 bits (1866), Expect = 7.98e-258
Identity = 346/395 (87.59%), Postives = 372/395 (94.18%), Query Frame = 0

Query: 1   MWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRK 60
           MWMLLERVRFTLDGLECNKIGVGYETFN QPDFCTSPFWSCLHNQLWNFREADLSRIGR 
Sbjct: 276 MWMLLERVRFTLDGLECNKIGVGYETFNSQPDFCTSPFWSCLHNQLWNFREADLSRIGRN 335

Query: 61  QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSI 120
           Q+PLYGVEGRFERINQHPNAGTHSFSIG+TEVLNTNLVIELRADDVEYVYQRSPGKIMSI
Sbjct: 336 QMPLYGVEGRFERINQHPNAGTHSFSIGITEVLNTNLVIELRADDVEYVYQRSPGKIMSI 395

Query: 121 SIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKL 180
           +IPTFEALTQFGVAT+ TKNTGE+EASYSLTFTCSKEVSLMEEQY++MKP E+ASRSFKL
Sbjct: 396 NIPTFEALTQFGVATITTKNTGELEASYSLTFTCSKEVSLMEEQYFLMKPKEVASRSFKL 455

Query: 181 YPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHS 240
           YPTTDQAAKYVC+AILKDADFSEVDRAECQFATT+TVLDNGSQITPFELPKKKENGF+ S
Sbjct: 456 YPTTDQAAKYVCSAILKDADFSEVDRAECQFATTSTVLDNGSQITPFELPKKKENGFVDS 515

Query: 241 IKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVIL 300
           IKL WK+ WG+++DFV GKSCRK C+ FFDFSCHIQY+CLSWLVLFGLFLA FPAV+V++
Sbjct: 516 IKLVWKKLWGNIVDFVIGKSCRKECARFFDFSCHIQYLCLSWLVLFGLFLAIFPAVVVVI 575

Query: 301 WVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHY--HRHGSRHHQNHGSGY-KR 360
           W+LHQKGLFDP+Y+WW DMF  KS PTR TWK+RGERKH   HRHGSRH+QNHGSGY KR
Sbjct: 576 WILHQKGLFDPVYEWWGDMFGAKSHPTRPTWKHRGERKHSQSHRHGSRHYQNHGSGYHKR 635

Query: 361 RSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 392
           RSHE HKKHKHS+RDTDYFLHHVHRKK K GHNRV
Sbjct: 636 RSHESHKKHKHSDRDTDYFLHHVHRKKDKGGHNRV 670

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JP367.7e-15063.64Protein HAPLESS 2 OS=Arabidopsis thaliana OX=3702 GN=HAP2 PE=1 SV=1[more]
Q5W6B99.8e-11354.11Protein HAPLESS 2-A OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2A PE=2 SV=1[more]
B9G4M91.0e-9352.38Protein HAPLESS 2-B OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2B PE=3 SV=1[more]
A4GRC64.7e-2233.05Hapless 2 OS=Chlamydomonas reinhardtii OX=3055 GN=HAP2 PE=1 SV=2[more]
A0A060A6824.9e-1924.18Hapless 2 OS=Tetrahymena thermophila OX=5911 GN=HAP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004143990.19.04e-292100.00protein HAPLESS 2 [Cucumis sativus][more]
XP_008437218.17.34e-27696.64PREDICTED: protein HAPLESS 2 isoform X1 [Cucumis melo][more]
KAA0042793.11.32e-27490.24protein HAPLESS 2 isoform X1 [Cucumis melo var. makuwa][more]
XP_038875720.12.39e-26892.09protein HAPLESS 2 [Benincasa hispida][more]
XP_008437219.13.60e-26493.80PREDICTED: protein HAPLESS 2 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0KQY83.66e-292100.00HAP2-GCS1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155520 P... [more]
A0A1S3ATM03.55e-27696.64protein HAPLESS 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1[more]
A0A5A7THM36.37e-27590.24Protein HAPLESS 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3ATH01.74e-26493.80protein HAPLESS 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1[more]
A0A6J1I2267.98e-25887.59protein HAPLESS 2 OS=Cucurbita maxima OX=3661 GN=LOC111468909 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018928Generative cell specific-1/HAP2 domainPFAMPF10699HAP2-GCS1coord: 2..279
e-value: 2.4E-75
score: 253.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 340..365
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 340..371
IPR040326HAP2/GCS1PANTHERPTHR31764PROTEIN HAPLESS 2coord: 1..385

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G21374Cucsat.G21374gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G21374.T5.E1Cucsat.G21374.T5.E1exon
Cucsat.G21374.T5.E2Cucsat.G21374.T5.E2exon
Cucsat.G21374.T5.E3Cucsat.G21374.T5.E3exon
Cucsat.G21374.T5.E4Cucsat.G21374.T5.E4exon
Cucsat.G21374.T5.E5Cucsat.G21374.T5.E5exon
Cucsat.G21374.T5.E6Cucsat.G21374.T5.E6exon
Cucsat.G21374.T5.E7Cucsat.G21374.T5.E7exon
Cucsat.G21374.T5.E8Cucsat.G21374.T5.E8exon
Cucsat.G21374.T5.E9Cucsat.G21374.T5.E9exon
Cucsat.G21374.T5.E10Cucsat.G21374.T5.E10exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G21374.T5.C1Cucsat.G21374.T5.C1CDS
Cucsat.G21374.T5.C2Cucsat.G21374.T5.C2CDS
Cucsat.G21374.T5.C3Cucsat.G21374.T5.C3CDS
Cucsat.G21374.T5.C4Cucsat.G21374.T5.C4CDS
Cucsat.G21374.T5.C5Cucsat.G21374.T5.C5CDS
Cucsat.G21374.T5.C6Cucsat.G21374.T5.C6CDS
Cucsat.G21374.T5.C7Cucsat.G21374.T5.C7CDS
Cucsat.G21374.T5.C8Cucsat.G21374.T5.C8CDS
Cucsat.G21374.T5.C9Cucsat.G21374.T5.C9CDS
Cucsat.G21374.T5.C10Cucsat.G21374.T5.C10CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G21374.T5Cucsat.G21374.T5-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061936 fusion of sperm to egg plasma membrane involved in double fertilization forming a zygote and endosperm
biological_process GO:0048235 pollen sperm cell differentiation
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0005886 plasma membrane