CsGy7G013495 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy7G013495
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Reverse transcriptase Ty1/copia-type domain-containing protein
Location	Gy14Chr7: 17544133 .. 17544485 (+)
RNA-Seq Expression	CsGy7G013495
Synteny	CsGy7G013495

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTATCGACAACAAATGGGTATTTCACATCAAACGAGCAGTTGATGGCTCCATACAACGCTACAAGGCTCGTTTGGTGGCAAAAGGGTTTCATCAACATCCTGAGGTTGACTTTTTTGAGACATTCAGTTCGGTCGTAAAGTCTTCAATGATTCGGATAATCTTAAGCTTGGTTGTCTCAAATCATTGGGTTCTTCGTCAACTTGACTTCAACAATGCTTTTCTCAATAGAGCACTGATAGAGGAAGTTTACATGTCACAGCCACCTAGGTTTGTTTACCCACAATATCTCGATTATGTTTGCAGATTGAGGAAAGCCATTTACAGCCTCAAAAAAGCTCCCCAAGCTTGA

mRNA sequence

ATGTTATCGACAACAAATGGGTATTTCACATCAAACGAGCAGTTGATGGCTCCATACAACGCTACAAGGCTCGTTTGGTGGCAAAAGGGTTTCATCAACATCCTGAGTTCGGTCGTAAAGTCTTCAATGATTCGGATAATCTTAAGCTTGGTTGTCTCAAATCATTGGGTTCTTCGTCAACTTGACTTCAACAATGCTTTTCTCAATAGAGCACTGATAGAGGAAGTTTACATGTCACAGCCACCTAGGTTTGTTTACCCACAATATCTCGATTATGTTTGCAGATTGAGGAAAGCCATTTACAGCCTCAAAAAAGCTCCCCAAGCTTGA

Coding sequence (CDS)

ATGTTATCGACAACAAATGGGTATTTCACATCAAACGAGCAGTTGATGGCTCCATACAACGCTACAAGGCTCGTTTGGTGGCAAAAGGGTTTCATCAACATCCTGAGTTCGGTCGTAAAGTCTTCAATGATTCGGATAATCTTAAGCTTGGTTGTCTCAAATCATTGGGTTCTTCGTCAACTTGACTTCAACAATGCTTTTCTCAATAGAGCACTGATAGAGGAAGTTTACATGTCACAGCCACCTAGGTTTGTTTACCCACAATATCTCGATTATGTTTGCAGATTGAGGAAAGCCATTTACAGCCTCAAAAAAGCTCCCCAAGCTTGA

Protein sequence

MLSTTNGYFTSNEQLMAPYNATRLVWWQKGFINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYLDYVCRLRKAIYSLKKAPQA*

Homology

[Close]

BLAST of CsGy7G013495 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.6e-18
Identity = 46/79 (58.23%), Postives = 55/79 (69.62%), Query Frame = 0


Query: 31   FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYL 90
            +    S V+KS+ IRI+L + V   W +RQLD NNAFL   L +EVYMSQPP FV     
Sbjct: 1017 YAETFSPVIKSTSIRIVLGVAVDRSWPIRQLDVNNAFLQGTLTDEVYMSQPPGFVDKDRP 1076

Query: 91   DYVCRLRKAIYSLKKAPQA 110
            DYVCRLRKAIY LK+AP+A
Sbjct: 1077 DYVCRLRKAIYGLKQAPRA 1095

[Close]

BLAST of CsGy7G013495 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.1e-16
Identity = 41/79 (51.90%), Postives = 55/79 (69.62%), Query Frame = 0


Query: 31   FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYL 90
            +    S V+KS+ IRI+L + V   W +RQLD NNAFL   L ++VYMSQPP F+     
Sbjct: 1034 YAETFSPVIKSTSIRIVLGVAVDRSWPIRQLDVNNAFLQGTLTDDVYMSQPPGFIDKDRP 1093

Query: 91   DYVCRLRKAIYSLKKAPQA 110
            +YVC+LRKA+Y LK+AP+A
Sbjct: 1094 NYVCKLRKALYGLKQAPRA 1112

[Close]

BLAST of CsGy7G013495 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.7e-09
Identity = 36/78 (46.15%), Postives = 45/78 (57.69%), Query Frame = 0


Query: 31  FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYL 90
           F  I S VVK + IR ILSL  S    + QLD   AFL+  L EE+YM QP  F      
Sbjct: 891 FDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVKTAFLHGDLEEEIYMEQPEGFEVAGKK 950

Query: 91  DYVCRLRKAIYSLKKAPQ 109
             VC+L K++Y LK+AP+
Sbjct: 951 HMVCKLNKSLYGLKQAPR 968

[Close]

BLAST of CsGy7G013495 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 53.1 bits (126), Expect = 2.3e-06
Identity = 32/80 (40.00%), Postives = 43/80 (53.75%), Query Frame = 0


Query: 27   WQKGFINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVY 86
            +Q  +    + V + S  R ILSLV+  +  + Q+D   AFLN  L EE+YM  P     
Sbjct: 967  YQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKTAFLNGTLKEEIYMRLPQGISC 1026

Query: 87   PQYLDYVCRLRKAIYSLKKA 107
                D VC+L KAIY LK+A
Sbjct: 1027 NS--DNVCKLNKAIYGLKQA 1044

[Close]

BLAST of CsGy7G013495 vs. ExPASy Swiss-Prot
Match: P25600 (Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY5A PE=5 SV=2)

HSP 1 Score: 45.8 bits (107), Expect = 3.6e-04
Identity = 21/47 (44.68%), Postives = 29/47 (61.70%), Query Frame = 0


Query: 61  LDFNNAFLNRALIEEVYMSQPPRFVYPQYLDYVCRLRKAIYSLKKAP 108
           +D + AFLN  + E +Y+ QPP FV  +  DYV  L   +Y LK+AP
Sbjct: 1   MDVDTAFLNSTMDEPIYVKQPPGFVNERNPDYVWELYGGMYGLKQAP 47

[Close]

BLAST of CsGy7G013495 vs. NCBI nr
Match: XP_016902198.1 (PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo] >XP_016902199.1 PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo] >XP_016902200.1 PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo] >XP_016902201.1 PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo] >XP_016902202.1 PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo])

HSP 1 Score: 95.9 bits (237), Expect = 1.43e-20
Identity = 47/77 (61.04%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 31  FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYL 90
           F    S V+K+S IR++LS+ V N W LRQLDFNNAFLN  L E VYM+QPP +V+P Y 
Sbjct: 81  FFETFSPVIKASTIRVVLSIAVPNGWPLRQLDFNNAFLNGHLEENVYMTQPPGYVHPSYP 140

Query: 91  DYVCRLRKAIYSLKKAP 107
           +YVC+L KAIY LK+AP
Sbjct: 141 NYVCKLNKAIYGLKQAP 157

[Close]

BLAST of CsGy7G013495 vs. NCBI nr
Match: KAA0026101.1 (putative mitochondrial protein [Cucumis melo var. makuwa] >TYJ96310.1 putative mitochondrial protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 2.59e-20
Identity = 48/79 (60.76%), Postives = 58/79 (73.42%), Query Frame = 0


Query: 31  FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYL 90
           F    S V+K+S IR++LS+ V+N W LRQLDFNNAFLN  L E VYM QPP +V+P Y 
Sbjct: 100 FFETFSPVIKASTIRVVLSIAVTNGWPLRQLDFNNAFLNGHLEENVYMMQPPGYVHPSYP 159

Query: 91  DYVCRLRKAIYSLKKAPQA 109
            YVC+L KAIY LK+AP A
Sbjct: 160 KYVCKLNKAIYGLKQAPCA 178

[Close]

BLAST of CsGy7G013495 vs. NCBI nr
Match: GEX05958.1 (retrovirus-related Pol polyprotein from transposon TNT 1-94 [Tanacetum cinerariifolium])

HSP 1 Score: 90.9 bits (224), Expect = 1.03e-19
Identity = 46/74 (62.16%), Postives = 57/74 (77.03%), Query Frame = 0


Query: 36  SSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYLDYVCR 95
           S + K   IR+IL L +S +W LRQLD NNAFLN  L EEVYM+QP  FV+PQY ++VC+
Sbjct: 38  SPITKPLTIRVILCLALSKNWPLRQLDINNAFLNGTLREEVYMAQPVGFVHPQYPNHVCK 97

Query: 96  LRKAIYSLKKAPQA 109
           LRKA+YSLK+AP A
Sbjct: 98  LRKALYSLKQAPTA 111

[Close]

BLAST of CsGy7G013495 vs. NCBI nr
Match: XP_014522498.1 (uncharacterized protein LOC106778999 [Vigna radiata var. radiata])

HSP 1 Score: 92.0 bits (227), Expect = 1.62e-19
Identity = 47/74 (63.51%), Postives = 56/74 (75.68%), Query Frame = 0


Query: 36  SSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYLDYVCR 95
           S VVK   +R+IL+L V N+W L QLD NNAFLNR L   VYM QPP FV P+YL++VCR
Sbjct: 20  SPVVKVVTVRVILTLAVMNYWPLHQLDVNNAFLNRHLTHPVYMEQPPWFVDPRYLNHVCR 79

Query: 96  LRKAIYSLKKAPQA 109
           LRKA+Y LK+AP A
Sbjct: 80  LRKALYGLKQAPLA 93

[Close]

BLAST of CsGy7G013495 vs. NCBI nr
Match: TYJ96957.1 (putative mitochondrial protein [Cucumis melo var. makuwa])

HSP 1 Score: 90.9 bits (224), Expect = 3.84e-19
Identity = 45/79 (56.96%), Postives = 60/79 (75.95%), Query Frame = 0


Query: 31  FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYL 90
           F    S VVK+S IR+IL+L V+N W LRQLDFNNAFLN  L + VY+ +PP +V+P + 
Sbjct: 85  FFETFSPVVKASTIRVILNLAVTNGWQLRQLDFNNAFLNGNLTKTVYIQKPPGYVHPSFP 144

Query: 91  DYVCRLRKAIYSLKKAPQA 109
           +YVC+L KAIY LK++P+A
Sbjct: 145 NYVCKLDKAIYGLKQSPRA 163

[Close]

BLAST of CsGy7G013495 vs. ExPASy TrEMBL
Match: A0A1S4E1U4 (uncharacterized mitochondrial protein AtMg00810-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC107991581 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 6.91e-21
Identity = 47/77 (61.04%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 31  FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYL 90
           F    S V+K+S IR++LS+ V N W LRQLDFNNAFLN  L E VYM+QPP +V+P Y 
Sbjct: 81  FFETFSPVIKASTIRVVLSIAVPNGWPLRQLDFNNAFLNGHLEENVYMTQPPGYVHPSYP 140

Query: 91  DYVCRLRKAIYSLKKAP 107
           +YVC+L KAIY LK+AP
Sbjct: 141 NYVCKLNKAIYGLKQAP 157

[Close]

BLAST of CsGy7G013495 vs. ExPASy TrEMBL
Match: A0A5D3B8X5 (Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00130 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.25e-20
Identity = 48/79 (60.76%), Postives = 58/79 (73.42%), Query Frame = 0


Query: 31  FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYL 90
           F    S V+K+S IR++LS+ V+N W LRQLDFNNAFLN  L E VYM QPP +V+P Y 
Sbjct: 100 FFETFSPVIKASTIRVVLSIAVTNGWPLRQLDFNNAFLNGHLEENVYMMQPPGYVHPSYP 159

Query: 91  DYVCRLRKAIYSLKKAPQA 109
            YVC+L KAIY LK+AP A
Sbjct: 160 KYVCKLNKAIYGLKQAPCA 178

[Close]

BLAST of CsGy7G013495 vs. ExPASy TrEMBL
Match: A0A2N9EIU9 (Reverse transcriptase Ty1/copia-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2605 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 6.83e-20
Identity = 49/102 (48.04%), Postives = 68/102 (66.67%), Query Frame = 0


Query: 16  MAPYNATRLVWWQKGFINIL--------SSVVKSSMIRIILSLVVSNHWVLRQLDFNNAF 75
           M P+ AT+  W  + FIN L        +S+VK   +RI+LSL   + W LRQLD +NAF
Sbjct: 1   MDPFLATKHAWLLRAFINRLVWTLMKCSASIVKPPTVRIVLSLAAQHQWSLRQLDISNAF 60

Query: 76  LNRALIEEVYMSQPPRFVYPQYLDYVCRLRKAIYSLKKAPQA 109
           L+  L E+VYM QPP FV P + D+VC+L+K++Y LK+AP+A
Sbjct: 61  LHGFLKEDVYMIQPPGFVDPTHPDHVCKLQKSLYGLKQAPRA 102

[Close]

BLAST of CsGy7G013495 vs. ExPASy TrEMBL
Match: A0A1S3VVV0 (uncharacterized protein LOC106778999 OS=Vigna radiata var. radiata OX=3916 GN=LOC106778999 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 7.87e-20
Identity = 47/74 (63.51%), Postives = 56/74 (75.68%), Query Frame = 0


Query: 36  SSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYLDYVCR 95
           S VVK   +R+IL+L V N+W L QLD NNAFLNR L   VYM QPP FV P+YL++VCR
Sbjct: 20  SPVVKVVTVRVILTLAVMNYWPLHQLDVNNAFLNRHLTHPVYMEQPPWFVDPRYLNHVCR 79

Query: 96  LRKAIYSLKKAPQA 109
           LRKA+Y LK+AP A
Sbjct: 80  LRKALYGLKQAPLA 93

[Close]

BLAST of CsGy7G013495 vs. ExPASy TrEMBL
Match: A0A2N9FKS9 (Integrase catalytic domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15774 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.55e-19
Identity = 50/117 (42.74%), Postives = 74/117 (63.25%), Query Frame = 0


Query: 1   MLSTTNGYFTSNEQLMAPYNATRLVWWQKGF--------INILSSVVKSSMIRIILSLVV 60
           +L   NG F  N  LM   +A++L    K          + +L+ V+K++ + ++LSL V
Sbjct: 515 ILWVPNGCFGQNTCLMDLSSASKLALLPKAIHRYLVSTTLTLLALVIKATTVHVVLSLAV 574

Query: 61  SNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQYLDYVCRLRKAIYSLKKAPQA 109
           +N W LRQLD  NAFLN  L E VYM QPP ++ P+Y ++VC+L+KA+Y LK+AP+A
Sbjct: 575 TNKWPLRQLDVKNAFLNGHLTESVYMEQPPGYIDPRYPNHVCQLKKALYGLKQAPRA 631

[Close]

BLAST of CsGy7G013495 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 57.4 bits (137), Expect = 8.5e-09
Identity = 33/80 (41.25%), Postives = 48/80 (60.00%), Query Frame = 0


Query: 31  FINILSSVVKSSMIRIILSLVVSNHWVLRQLDFNNAFLNRALIEEVYMSQPPRFVYPQ-- 90
           FI   S V K + +++IL++    ++ L QLD +NAFLN  L EE+YM  PP +   Q  
Sbjct: 163 FIETFSPVCKLTSVKLILAISAIYNFTLHQLDISNAFLNGDLDEEIYMKLPPGYAARQGD 222

Query: 91  --YLDYVCRLRKAIYSLKKA 107
               + VC L+K+IY LK+A
Sbjct: 223 SLPPNAVCYLKKSIYGLKQA 242

The following BLAST results are available for this feature:

BLAST of CsGy7G013495 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZT94	2.6e-18	58.23	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]
Q94HW2	1.1e-16	51.90	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
P10978	3.7e-09	46.15	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	2.3e-06	40.00	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]
P25600	3.6e-04	44.68	Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain AT...	[more]

BLAST of CsGy7G013495 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016902198.1	1.43e-20	61.04	PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucu...	[more]
KAA0026101.1	2.59e-20	60.76	putative mitochondrial protein [Cucumis melo var. makuwa] >TYJ96310.1 putative m...	[more]
GEX05958.1	1.03e-19	62.16	retrovirus-related Pol polyprotein from transposon TNT 1-94 [Tanacetum cinerarii...	[more]
XP_014522498.1	1.62e-19	63.51	uncharacterized protein LOC106778999 [Vigna radiata var. radiata]	[more]
TYJ96957.1	3.84e-19	56.96	putative mitochondrial protein [Cucumis melo var. makuwa]	[more]

BLAST of CsGy7G013495 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E1U4	6.91e-21	61.04	uncharacterized mitochondrial protein AtMg00810-like isoform X2 OS=Cucumis melo ...	[more]
A0A5D3B8X5	1.25e-20	60.76	Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A2N9EIU9	6.83e-20	48.04	Reverse transcriptase Ty1/copia-type domain-containing protein OS=Fagus sylvatic...	[more]
A0A1S3VVV0	7.87e-20	63.51	uncharacterized protein LOC106778999 OS=Vigna radiata var. radiata OX=3916 GN=LO...	[more]
A0A2N9FKS9	1.55e-19	42.74	Integrase catalytic domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB...	[more]

BLAST of CsGy7G013495 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23160.1	8.5e-09	41.25	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 30..108 e-value: 6.5E-17 score: 61.9
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 23..109
None	No IPR available	PANTHER	PTHR11439:SF331	SERINE/THREONINE-PROTEIN KINASE, ACTIVE SITE PROTEIN-RELATED	coord: 23..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy7G013495.1	CsGy7G013495.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
molecular_function	GO:0005488	binding
molecular_function	GO:0004518	nuclease activity
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0008233	peptidase activity