Tan0014516.1 (mRNA) Snake gourd v1

Overview


Name	Tan0014516.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	LG01: 27201085 .. 27202641 (-)
Sequence length	1017
RNA-Seq Expression	Tan0014516.1
Synteny	Tan0014516.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGAAATTTTCAAGCACCTCGTCGGTTCCGCCGGAGCAAGCGGCTACGGCTCCAAATCCACGGCCGAAGAGGTTACTCAAGCATGTTCTCATCTGCACTCCATCACCGCTATAATCACAGGTTCTCTTCTTTCAATTCAGAATTCCGATTTCATTACATTATACGTTAAAATTCTTTTGTGAACGAATTTTGGCGCAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTTTTAGCCAAGCGCGGCGCCAGGATCGTCATTCCGGCTCGCAACATCAAAGCCGCCGAGGAAGTCAAGGCGCGAATCGTATCGGAATCGGAATGTTTCGATCATTCACGAATCACGGTGATGGCTCTCGATCTAAGTTCTCTGAATTCCGTCACAAACTTCGTCTCCAAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATGTAAATCATCCAAAAATTAAACATCGCTTAATGCACTCCGATGATTCGAACAAACAAACGACCTCAATTGATTTAAGAAACTAAAAGACGGTTTCGTTCTGATTTTCAGAAACAATGCCGGCAGATTCTGTTACGAACATGCAATTTCTGAAGACGGAATGGAGATGACGTTCGCTACTAATTATTTAGGTAAGTACGCGTTTAACAAATTTATTCTCATCGCTAATCATGAGGCCTTCAACCATTATTGATCGAATCGAGCAAGCAGGTCATTTTCTGTTGACGAAACTTTTGTTGAATAAGATGATCGAAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTTTCCGGCGACATATTCGAATATCTCGGCCAGATAAGCTTGAACAACAGGCATGATTAATTTTCCCTCCAGATAAGCTTGAACAACAGGCATGATTAATTTTCCCTTGCTTACGTATTCATGGCGTTCTGATACCGATACCGATATTCACTCGATCGCAGCAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCGAATGTCTTGCACACCGTCCAACTCGCTCACAGACTCCAGGTTCTTCCATAGATATCAATCGGTATTTTCATAGAACGATTTCACGTTCGACTTGGCTTTTGACAAAATCGAAACAGGAAATGGAGGCAAATGTTACAGTAAACTGTGTTCATCCGGGCGTGGTGAAAACCAAATTGAACAGAGACCGTGAAGGCTTCGTCACAGGTATCGATCAAAGCTCCTACACGGAAATTAATCAATCTGAAACGTTTTTGTATAACGAATTAATTAATGAGAAAATTATGCAGATTTGATTTTCTACATGGCGTCCAAATCAAAATTGTTGAAGACAGTTTCTCAAGGTGCAGCAACAAGTTGTTACGTAGCCGCGAATCGGAACGTGGAAAATGTGAGTGGAAAATACTTTGCGGATTGCAACGAACAAGGAAGCTGTGAATCCAAATTAGGTGCCCAATTTACGCACTTAGCTGCACGCCTATGGTCCACCTCTGAAACCATCGTCTCCACTTTTACAGGTAACCCTGAACTCTAA

mRNA sequence

ATGCTTGAAATTTTCAAGCACCTCGTCGGTTCCGCCGGAGCAAGCGGCTACGGCTCCAAATCCACGGCCGAAGAGGTTACTCAAGCATGTTCTCATCTGCACTCCATCACCGCTATAATCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTTTTAGCCAAGCGCGGCGCCAGGATCGTCATTCCGGCTCGCAACATCAAAGCCGCCGAGGAAGTCAAGGCGCGAATCGTATCGGAATCGGAATGTTTCGATCATTCACGAATCACGGTGATGGCTCTCGATCTAAGTTCTCTGAATTCCGTCACAAACTTCGTCTCCAAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATAAACAATGCCGGCAGATTCTGTTACGAACATGCAATTTCTGAAGACGGAATGGAGATGACGTTCGCTACTAATTATTTAGGTCATTTTCTGTTGACGAAACTTTTGTTGAATAAGATGATCGAAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTTTCCGGCGACATATTCGAATATCTCGGCCAGATAAGCTTGAACAACAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCGAATGTCTTGCACACCGTCCAACTCGCTCACAGACTCCAGGAAATGGAGGCAAATGTTACAGTAAACTGTGTTCATCCGGGCGTGGTGAAAACCAAATTGAACAGAGACCGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAAATTGTTGAAGACAGTTTCTCAAGGTGCAGCAACAAGTTGTTACGTAGCCGCGAATCGGAACGTGGAAAATGTGAGTGGAAAATACTTTGCGGATTGCAACGAACAAGGAAGCTGTGAATCCAAATTAGGTGCCCAATTTACGCACTTAGCTGCACGCCTATGGTCCACCTCTGAAACCATCGTCTCCACTTTTACAGGTAACCCTGAACTCTAA

Coding sequence (CDS)

ATGCTTGAAATTTTCAAGCACCTCGTCGGTTCCGCCGGAGCAAGCGGCTACGGCTCCAAATCCACGGCCGAAGAGGTTACTCAAGCATGTTCTCATCTGCACTCCATCACCGCTATAATCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTTTTAGCCAAGCGCGGCGCCAGGATCGTCATTCCGGCTCGCAACATCAAAGCCGCCGAGGAAGTCAAGGCGCGAATCGTATCGGAATCGGAATGTTTCGATCATTCACGAATCACGGTGATGGCTCTCGATCTAAGTTCTCTGAATTCCGTCACAAACTTCGTCTCCAAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATAAACAATGCCGGCAGATTCTGTTACGAACATGCAATTTCTGAAGACGGAATGGAGATGACGTTCGCTACTAATTATTTAGGTCATTTTCTGTTGACGAAACTTTTGTTGAATAAGATGATCGAAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTTTCCGGCGACATATTCGAATATCTCGGCCAGATAAGCTTGAACAACAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCGAATGTCTTGCACACCGTCCAACTCGCTCACAGACTCCAGGAAATGGAGGCAAATGTTACAGTAAACTGTGTTCATCCGGGCGTGGTGAAAACCAAATTGAACAGAGACCGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAAATTGTTGAAGACAGTTTCTCAAGGTGCAGCAACAAGTTGTTACGTAGCCGCGAATCGGAACGTGGAAAATGTGAGTGGAAAATACTTTGCGGATTGCAACGAACAAGGAAGCTGTGAATCCAAATTAGGTGCCCAATTTACGCACTTAGCTGCACGCCTATGGTCCACCTCTGAAACCATCGTCTCCACTTTTACAGGTAACCCTGAACTCTAA

Protein sequence

MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGARIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNLLINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNIHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADCNEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPEL

Homology

[Close]

BLAST of Tan0014516.1 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 7.0e-67
Identity = 150/332 (45.18%), Postives = 213/332 (64.16%), Query Frame = 0


Query: 2   LEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGAR 61
           +EI+  + G AG SGYGS STAE+VT +    H +TAIITG TSGIG E ARVL  RGA 
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKH-LTAIITGGTSGIGLEAARVLGMRGAH 60

Query: 62  IVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNLL 121
           ++I +RN KAA + K  I+   + + ++RI  + LDLSS+ SV +F+ +F +L++PLN+L
Sbjct: 61  VIIASRNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNIL 120

Query: 122 INNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNI 181
           INNAG       +SEDG+E  FATN++GHFLLT LLL+KM  +A+ +GI+GRIVN++S  
Sbjct: 121 INNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIA 180

Query: 182 H--SWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCV 241
           H  ++  G +F+Y+     +   Y   +AY  SKLAN+LH+  L+ +LQE   N+T+N V
Sbjct: 181 HTYTYTEGIMFDYIN----DPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSV 240

Query: 242 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFAD 301
           HPG++ T L R        +    A    L K + QGAAT+CYVA + ++++V+GKYFAD
Sbjct: 241 HPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFAD 300

Query: 302 CNEQGSCESKLGAQFTHLAARLWSTSETIVST 332
           CN        +    T LA +LW  S  +V +
Sbjct: 301 CNVTTPSNFAID---TTLADKLWDFSIKLVES 318

[Close]

BLAST of Tan0014516.1 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-66
Identity = 150/332 (45.18%), Postives = 211/332 (63.55%), Query Frame = 0


Query: 2   LEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGAR 61
           +EI+  + G AG SGYGS STAE+VT +    H +TAIITG TSGIG E ARVL  RGA 
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKH-LTAIITGGTSGIGLEAARVLGMRGAH 60

Query: 62  IVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNLL 121
           ++I ARN KAA + K  I+   + + ++RI  + LDLSS+ SV +F+ +F +L++PLN+L
Sbjct: 61  VIIAARNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNIL 120

Query: 122 INNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNI 181
           INNAG       +SEDG+E  FATN++GHFLLT LLL+KM  +A+ +GI+GRIVN++S  
Sbjct: 121 INNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIA 180

Query: 182 H--SWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCV 241
           H  ++  G +F+Y+     +   Y   +AY  SKLAN+LH+  L+ +LQE   N+T+N V
Sbjct: 181 HTYTYTEGIMFDYIN----DPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSV 240

Query: 242 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFAD 301
           HPG++ T L R        +    A    L K + QGAAT+CYVA + +++ V+GKYF D
Sbjct: 241 HPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTD 300

Query: 302 CNEQGSCESKLGAQFTHLAARLWSTSETIVST 332
           CN        +    T LA +LW  S  +V +
Sbjct: 301 CNVTTPSNFAID---TTLADKLWDFSIKLVDS 318

[Close]

BLAST of Tan0014516.1 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.9e-59
Identity = 144/327 (44.04%), Postives = 199/327 (60.86%), Query Frame = 0


Query: 10  GSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGARIVIPARNI 69
           GS GASG+ S+STAEEVT        +TAI+TGA+SGIG ETARVL+ RG  +V+  RN 
Sbjct: 5   GSKGASGFSSRSTAEEVTHGVDGT-GLTAIVTGASSGIGVETARVLSLRGVHVVMAVRNT 64

Query: 70  KAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNLLINNAGRFC 129
            +  +VK  IV +      +++ VM LDLSS+ SV  F S+++S  LPLNLLINNAG   
Sbjct: 65  DSGAKVKEDIVKQ---VPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 124

Query: 130 YEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNIH--SWFSG 189
               +S+D +E+ FATN+LGHFLLTKLLL+ M  T++ +  +GRIVN++S  H  S+  G
Sbjct: 125 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 184

Query: 190 DIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVHPGVVKTK 249
             F+ +   S     Y + RAY  SKL NVLH  +L  +L+E   N+T N +HPG + T 
Sbjct: 185 VRFDKINDKS----SYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTN 244

Query: 250 LNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADCNEQGSCE 309
           L R    ++   +  +A    +LK+V QGAAT+CYVA N  V  VSG+YF D N     +
Sbjct: 245 LGRYFNPYLAVAVGAVA--KYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSN---IAK 304

Query: 310 SKLGAQFTHLAARLWSTSETIVSTFTG 335
                + T LA ++W  S  +  + +G
Sbjct: 305 PLPLVKDTELAKKVWDFSTKLTDSQSG 318

[Close]

BLAST of Tan0014516.1 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 7.3e-56
Identity = 139/319 (43.57%), Postives = 191/319 (59.87%), Query Frame = 0


Query: 13  GASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGARIVIPARNIKAA 72
           G SG+   STAE+VT        +TAI+TGA+SGIG ET RVLA RGA +++  RN+ AA
Sbjct: 9   GVSGFSGSSTAEQVTHGID-ATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  EEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNLLINNAGRFCYEH 132
           ++VK  I+ +      +++  + LDLSSL+SV  F S+F S   PLN+LINNAG      
Sbjct: 69  KDVKDTILKD---IPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPF 128

Query: 133 AISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNIH--SWFSGDIF 192
            +S+D +E+ FATN++GHFLLT LLL+ M +T + +  +GRIVNV S  H  ++  G  F
Sbjct: 129 KLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF 188

Query: 193 EYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVHPGVVKTKLNR 252
           + +   S     Y+  RAY  SKLANVLH  QL   L+E   N+T N +HPG + T L R
Sbjct: 189 DKINDQS----SYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR 248

Query: 253 DREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADCNEQGSCESKL 312
                V  LI  +     +LK V QGAAT+CYVA +  V+ VSG+YF+D N     ++  
Sbjct: 249 HNSA-VNGLINVIG--KLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVY---KTTP 308

Query: 313 GAQFTHLAARLWSTSETIV 330
             +   LA +LW  S  +V
Sbjct: 309 HGKDVDLAKKLWDFSINLV 313

[Close]

BLAST of Tan0014516.1 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.3e-39
Identity = 121/309 (39.16%), Postives = 171/309 (55.34%), Query Frame = 0


Query: 33  LHSITAIITGATSGIGEETARVLAKRGARIVIPARNIKAAEEVKARIVSE----SECFDH 92
           +H  T +ITGA SG+G  TA  L + GAR+++  R+   AEE   ++  E    +EC   
Sbjct: 41  MHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPE 100

Query: 93  ------SRITVMALDLSSLNSVTNFVSKFESLHLPLNLLINNAGRFCYEHAISEDGMEMT 152
                   + V  LDL+SL SV  F  +       L++LINNAG F   +  +EDG EM 
Sbjct: 101 PGVSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQ 160

Query: 153 FATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNIHSWFSGDI-FEYLGQISLNNR 212
           F  N+LGHFLLT LLL  +  +A S     RIV V+S ++ +  GDI F+ L     + +
Sbjct: 161 FGVNHLGHFLLTNLLLGLLKSSAPS-----RIVVVSSKLYKY--GDINFDDLN----SEQ 220

Query: 213 EYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVHPGVVKTKLNRD-REGFVTDLI 272
            Y+ +  Y+ SKLAN+L T +LA RL+    NVTVN +HPG+V+T L R      +   +
Sbjct: 221 SYNKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPL 280

Query: 273 FYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADCNEQGSCESKLGAQFTHLAAR 330
           F + S +   KT  +GA TS Y+A++  VE VSG+YF DC E+   E    A    +A +
Sbjct: 281 FNLVSWA-FFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEE---ELLPKAMDESVARK 332

[Close]

BLAST of Tan0014516.1 vs. NCBI nr
Match: XP_022923251.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita moschata] >KAG7015802.1 Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564.7 bits (1454), Expect = 5.4e-157
Identity = 285/337 (84.57%), Postives = 312/337 (92.58%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+FK+LVGSAGASGYGSKSTAEEV+++C  ++SITAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RI+IPARN+KAAEE KARIVSES+CFDHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDG+EMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVH 240
           IHSWFSGDIFEYLGQIS  NR YD+TRAYAFSKLANVLHTVQLAH+LQEM+ANVTVNCVH
Sbjct: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240

Query: 241 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADC 300
           PGVVKT LNR+REGFVTDLIFYMASKS+LLKT+ +GAATSCYVA NRNVENV+G YFADC
Sbjct: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 300

Query: 301 NEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           N+QGSCE K   +  HLA  LWS SETIVST TG P+
Sbjct: 301 NDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 333

[Close]

BLAST of Tan0014516.1 vs. NCBI nr
Match: XP_011650304.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >KAE8650011.1 hypothetical protein Csa_011335 [Cucumis sativus])

HSP 1 Score: 562.8 bits (1449), Expect = 2.1e-156
Identity = 289/340 (85.00%), Postives = 311/340 (91.47%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+ K+LVGSAGASGYGSKSTAEEVTQ+ +HL+SITAI+TGATSGIGEETARVLAK GA
Sbjct: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKGGA 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RIVIPARN+KAAEE KARIVSE ECFDHSRITVM LDLSSLNSV NFVS+FESL+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDHSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHAISEDG+EMTFATNYLGHFLLTKLLLNKMIETA+STGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAESTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISLNN---REYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVN 240
           IHSWFSGD+FEYL QIS NN   REYDATRAYAFSKLAN+LHTVQLAHRLQEM+ANVTVN
Sbjct: 181 IHSWFSGDVFEYLSQISRNNNKYREYDATRAYAFSKLANLLHTVQLAHRLQEMKANVTVN 240

Query: 241 CVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYF 300
           CVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKT+ Q AATSCYVA +R VENV+GKYF
Sbjct: 241 CVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKYF 300

Query: 301 ADCNEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           ADCNEQGSCES    +  HLA RLWSTSE IVS  TG P+
Sbjct: 301 ADCNEQGSCES--NTKSAHLAERLWSTSEIIVSNLTGKPQ 338

[Close]

BLAST of Tan0014516.1 vs. NCBI nr
Match: XP_023552712.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562.0 bits (1447), Expect = 3.5e-156
Identity = 283/337 (83.98%), Postives = 311/337 (92.28%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+FK+LVGSAGASGYGSKSTAEEV+++C  ++SITAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RI+IPARN+KAAEE KARIVSES+CFDHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDG+EMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVH 240
           IHSWFSGDIFEYLGQIS  NR YD+TRAYAFSKLANVLHTVQLAH+LQEM+ANVTVNCVH
Sbjct: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240

Query: 241 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADC 300
           PGVVKT LNR+REGFVTDLIFYM SKS+LLKT+ +GAAT+CYVA NRNVENV+G YFADC
Sbjct: 241 PGVVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFADC 300

Query: 301 NEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           N+QGSCE K   +  HLA  LWS SETIVST TG P+
Sbjct: 301 NDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 333

[Close]

BLAST of Tan0014516.1 vs. NCBI nr
Match: XP_022923250.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6577763.1 Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560.5 bits (1443), Expect = 1.0e-155
Identity = 285/338 (84.32%), Postives = 313/338 (92.60%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+FK+LVGSAGASGYGSKSTAEEV+++C  ++SITAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RI+IPARN+KAAEE KARIVSES+CFDHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDG+EMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQIS-LNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYLGQIS  N+R YD+TRAYAFSKLANVLHTVQLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFAD 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLKT+ +GAATSCYVA NRNVENV+G YFAD
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           CN+QGSCE K   +  HLA  LWS SETIVST TG P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Tan0014516.1 vs. NCBI nr
Match: XP_022965266.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 558.9 bits (1439), Expect = 3.0e-155
Identity = 283/337 (83.98%), Postives = 310/337 (91.99%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+FK+LVGSAGASGYGSKSTAEEV+++C  ++SITAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RI+IPARN+KAAEE KARIVSES CFD SRITVMALDLSS+ SV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA+SEDG+EMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVH 240
           IHSWFSGDIFEYLGQIS  NR YD+TRAYAFSKLANVLHTVQLAH+LQEM+ANVTVNCVH
Sbjct: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240

Query: 241 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADC 300
           PGVVKT LNR+REGFVTDLIFYMASKS+LLK + +GAATSCYVA NRNVENV+GKYFADC
Sbjct: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADC 300

Query: 301 NEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           N+QGSCE K   +  HLA  LWS SETIVST TG P+
Sbjct: 301 NDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 333

[Close]

BLAST of Tan0014516.1 vs. ExPASy TrEMBL
Match: A0A6J1E6B3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.6e-157
Identity = 285/337 (84.57%), Postives = 312/337 (92.58%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+FK+LVGSAGASGYGSKSTAEEV+++C  ++SITAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RI+IPARN+KAAEE KARIVSES+CFDHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDG+EMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVH 240
           IHSWFSGDIFEYLGQIS  NR YD+TRAYAFSKLANVLHTVQLAH+LQEM+ANVTVNCVH
Sbjct: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240

Query: 241 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADC 300
           PGVVKT LNR+REGFVTDLIFYMASKS+LLKT+ +GAATSCYVA NRNVENV+G YFADC
Sbjct: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 300

Query: 301 NEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           N+QGSCE K   +  HLA  LWS SETIVST TG P+
Sbjct: 301 NDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 333

[Close]

BLAST of Tan0014516.1 vs. ExPASy TrEMBL
Match: A0A6J1EB90 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.9e-156
Identity = 285/338 (84.32%), Postives = 313/338 (92.60%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+FK+LVGSAGASGYGSKSTAEEV+++C  ++SITAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RI+IPARN+KAAEE KARIVSES+CFDHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDG+EMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQIS-LNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYLGQIS  N+R YD+TRAYAFSKLANVLHTVQLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFAD 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLKT+ +GAATSCYVA NRNVENV+G YFAD
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           CN+QGSCE K   +  HLA  LWS SETIVST TG P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Tan0014516.1 vs. ExPASy TrEMBL
Match: A0A6J1HL83 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.4e-155
Identity = 283/337 (83.98%), Postives = 310/337 (91.99%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+FK+LVGSAGASGYGSKSTAEEV+++C  ++SITAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RI+IPARN+KAAEE KARIVSES CFD SRITVMALDLSS+ SV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA+SEDG+EMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVH 240
           IHSWFSGDIFEYLGQIS  NR YD+TRAYAFSKLANVLHTVQLAH+LQEM+ANVTVNCVH
Sbjct: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240

Query: 241 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADC 300
           PGVVKT LNR+REGFVTDLIFYMASKS+LLK + +GAATSCYVA NRNVENV+GKYFADC
Sbjct: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADC 300

Query: 301 NEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           N+QGSCE K   +  HLA  LWS SETIVST TG P+
Sbjct: 301 NDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 333

[Close]

BLAST of Tan0014516.1 vs. ExPASy TrEMBL
Match: A0A1S3BLF4 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491110 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.2e-154
Identity = 285/341 (83.58%), Postives = 309/341 (90.62%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLE+ K+LVGSAGASGYGS STAEEVTQ+ +HL+SITAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           RIVIPARN+KAAEE KARIVSE ECFD+SRITVM LDLSSLNSV NFVS+FE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDG+EMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISL----NNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTV 240
           IHSWFSGDIFEYL QIS     NNREYDATRAY+FSKLAN+LHTVQLAHRLQEM+ANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKY 300
           NCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKT+ Q AATSCYVA +R VENV+GKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FADCNEQGSCESKLGAQFTHLAARLWSTSETIVSTFTGNPE 338
           F DCNEQGSCES    +  HLA RLWS SE I+S  TGNP+
Sbjct: 301 FEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 339

[Close]

BLAST of Tan0014516.1 vs. ExPASy TrEMBL
Match: A0A6J1CW75 (short-chain dehydrogenase TIC 32, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014858 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.6e-154
Identity = 290/339 (85.55%), Postives = 305/339 (89.97%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           MLEI K+LVGSAGASGYGSKSTA EVTQ+C HL S+TAIITGATSGIGEETARVLAKRGA
Sbjct: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARNIKAAEEVKARI--VSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPL 120
           RIV+PARN+KAAEE KARI   SESEC DHSRITVM LDLSSLNSV NFVSKFESL+LPL
Sbjct: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGMEMTFATNYL-----------GHFLLTKLLLNKMIETAKS 180
           NLLINNAGRFCYEHAISEDG+EMTF TNYL           GHFLLTKLLLNKMIETAK 
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYIGEQILFSGHFLLTKLLLNKMIETAKL 180

Query: 181 TGIQGRIVNVTSNIHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRL 240
           TGIQGRIVN+TSNIHSWFSGDIFEYLG+IS +NREYDATRAYA SKLANVLHTVQLAHRL
Sbjct: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNREYDATRAYALSKLANVLHTVQLAHRL 240

Query: 241 QEMEANVTVNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANR 300
           QE EANVTVNCVHPGVV+TKLNRDREGFVTDLIFYMASK+KLLKT+ QGAATSCYVA NR
Sbjct: 241 QETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNR 300

Query: 301 NVENVSGKYFADCNEQGSCESKLGAQFTHLAARLWSTSE 327
           NVENV+GKYFADCNEQGSCESKLG +   LAARLWS SE
Sbjct: 301 NVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 339

[Close]

BLAST of Tan0014516.1 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 424.5 bits (1090), Expect = 8.1e-119
Identity = 226/330 (68.48%), Postives = 267/330 (80.91%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           M+E  K+L+G+AGASG+GSKSTAEEVT+ C  L SITA+ITGATSGIG ETARVLAKRGA
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCD-LRSITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           R++ PARN+KAAEE K RIVSE   F  + I VM LDLSS+ SV NFV+ FESL LPLNL
Sbjct: 61  RLIFPARNVKAAEEAKERIVSE---FPETEIVVMKLDLSSIASVRNFVADFESLDLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAG+  +EHAISEDG+EMTFATNYLGHFLLT LLLNKMI+TA+ TG+QGRIVNVTS 
Sbjct: 121 LINNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSG 180

Query: 181 IHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVH 240
           IH WFSGD+ EYL  IS    ++DATRAYA SKLANVLHT +L+ RLQ++ ANVTVNCVH
Sbjct: 181 IHGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVH 240

Query: 241 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADC 300
           PGVV+T+L RDREG +TDL+F++A  SKL+KTV Q AAT+CYVA N  + NVSGKYF DC
Sbjct: 241 PGVVRTRLTRDREGLLTDLVFFLA--SKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDC 300

Query: 301 NEQGSCESKLGAQFTHLAARLWSTSETIVS 331
           NE  +  S LG   +  A +LW+ SE +V+
Sbjct: 301 NE--TTPSGLGTNSSE-ATKLWAASEILVT 321

[Close]

BLAST of Tan0014516.1 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 419.9 bits (1078), Expect = 2.0e-117
Identity = 222/335 (66.27%), Postives = 263/335 (78.51%), Query Frame = 0


Query: 1   MLEIFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGA 60
           M+E  KHL+GS G SG+GS+STA+ VT   S L S+TAIITGATSGIG ETARVLAKRGA
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVT-CNSDLRSLTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNL 120
           R+V+PAR++K AEE KARI+SE   F  + I VM LDLSSL SV  FV  FESL+LPLN+
Sbjct: 61  RLVLPARSVKTAEETKARILSE---FPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNI 120

Query: 121 LINNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAG++ ++HA+SEDG+EMTFATNYLGHFLLTKLLL KMIETA  TG+QGRIVNVTS 
Sbjct: 121 LINNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSV 180

Query: 181 IHSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVH 240
           +HSWFSGD+ +YL  IS NNR YDATRAYA SKLANVLHTV+L+  L +M+ANVT NCVH
Sbjct: 181 VHSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVH 240

Query: 241 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADC 300
           PG+VKT+L RDREG VTDL+F++   SKLLK+V Q AAT+CYVA +  + NV GKYF+DC
Sbjct: 241 PGIVKTRLTRDREGVVTDLVFFLT--SKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDC 300

Query: 301 NE-----QGSCESKLGAQFTHLAARLWSTSETIVS 331
           NE      GSC  K        A RLW+ S+ +VS
Sbjct: 301 NEARSSKSGSCNLK--------AQRLWTASDLLVS 321

[Close]

BLAST of Tan0014516.1 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.1e-80
Identity = 168/328 (51.22%), Postives = 229/328 (69.82%), Query Frame = 0


Query: 6   KHLVGSAGASGYGSKSTAEEVTQ----ACSHLHSITAIITGATSGIGEETARVLAKRGAR 65
           ++L G AG +G+GS+STAE+VTQ     CSHL   TAIITG TSGIG ETARVLAKRG R
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHL---TAIITGGTSGIGAETARVLAKRGVR 65

Query: 66  IVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNLL 125
           +V+  R++K AE VK RI+ E+   D   I +  +DLSSL+SV  F S+F S  LPLN+L
Sbjct: 66  VVMAVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNIL 125

Query: 126 INNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNI 185
           INNAG F      SE+ +E+TFATN+LGH+LLT++L+ KMI+TA+ +GI+GRI+N++S I
Sbjct: 126 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 185

Query: 186 HSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVHP 245
           H+W   D F +   +   +R Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N VHP
Sbjct: 186 HNWVKPDCFSFPKLLHPISR-YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHP 245

Query: 246 GVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADCN 305
           G+VKT + R  +G  TD +F +A  SKLLK++SQGAAT+CYVA +   + +SGKYFADCN
Sbjct: 246 GIVKTGIIRAHKGLFTDSLFLIA--SKLLKSISQGAATTCYVALSNETKGLSGKYFADCN 305

Query: 306 EQGSCESKLGAQFTHLAARLWSTSETIV 330
           E    +    A   ++A +L + S  ++
Sbjct: 306 ETNCSDL---ANDEYVALKLCTQSRALI 321

[Close]

BLAST of Tan0014516.1 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 268.5 bits (685), Expect = 7.5e-72
Identity = 158/330 (47.88%), Postives = 219/330 (66.36%), Query Frame = 0


Query: 4   IFKHLVGSAGASGYGSKSTAEEVTQACSHLHSITAIITGATSGIGEETARVLAKRGARIV 63
           ++  + G  G SG+GS STAEEVTQ      ++TAIITG T GIG ETARVL+KRGA +V
Sbjct: 3   LYSLITGRRGPSGFGSASTAEEVTQGID-ATNLTAIITGGTGGIGMETARVLSKRGAHVV 62

Query: 64  IPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNLLIN 123
           I ARN+ AAE  K  I+ ++    ++R+T++ LDLSS+ S+  FV +F +LHLPLNLLIN
Sbjct: 63  IGARNMGAAENAKTEILRQNA---NARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLIN 122

Query: 124 NAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTS--NI 183
           NAG     + +SEDG+E+ FATN++GHFLLT LLL+ M  TAK++G++GRI+NV+S  +I
Sbjct: 123 NAGVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHI 182

Query: 184 HSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVHP 243
           +++  G  F+ +  I      Y   RAY  SKLAN+LH  +L+ +LQE   N+T N VHP
Sbjct: 183 YTYQEGIQFDSINDIC----SYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHP 242

Query: 244 GVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQGAATSCYVAANRNVENVSGKYFADCN 303
           G++ T L +     +  L F+      L K + QGAAT+CYVA + +V+ V+GKYFADCN
Sbjct: 243 GLILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCN 302

Query: 304 EQGSCESKLGAQFTHLAARLWSTSETIVST 332
           E     SKL    T LA +LW  S  ++++
Sbjct: 303 E--VTPSKLARDET-LAQKLWDFSVKLINS 318

[Close]

BLAST of Tan0014516.1 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 7.7e-69
Identity = 146/274 (53.28%), Postives = 196/274 (71.53%), Query Frame = 0


Query: 6   KHLVGSAGASGYGSKSTAEEVTQ----ACSHLHSITAIITGATSGIGEETARVLAKRGAR 65
           ++L G AG +G+GS+STAE+VTQ     CSHL   TAIITG TSGIG ETARVLAKRG R
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHL---TAIITGGTSGIGAETARVLAKRGVR 65

Query: 66  IVIPARNIKAAEEVKARIVSESECFDHSRITVMALDLSSLNSVTNFVSKFESLHLPLNLL 125
           +V+  R++K AE VK RI+ E+   D   I +  +DLSSL+SV  F S+F S  LPLN+L
Sbjct: 66  VVMAVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNIL 125

Query: 126 INNAGRFCYEHAISEDGMEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNI 185
           INNAG F      SE+ +E+TFATN+LGH+LLT++L+ KMI+TA+ +GI+GRI+N++S I
Sbjct: 126 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 185

Query: 186 HSWFSGDIFEYLGQISLNNREYDATRAYAFSKLANVLHTVQLAHRLQEMEANVTVNCVHP 245
           H+W   D F +   +   +R Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N VHP
Sbjct: 186 HNWVKPDCFSFPKLLHPISR-YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHP 245

Query: 246 GVVKTKLNRDREGFVTDLIFYMASKSKLLKTVSQ 276
           G+VKT + R  +G  TD +F +A  SKLLK++SQ
Sbjct: 246 GIVKTGIIRAHKGLFTDSLFLIA--SKLLKSISQ 270

The following BLAST results are available for this feature:

BLAST of Tan0014516.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	7.0e-67	45.18	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	1.2e-66	45.18	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	1.9e-59	44.04	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	7.3e-56	43.57	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q9HBH5	1.3e-39	39.16	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of Tan0014516.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923251.1	5.4e-157	84.57	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita mosch...	[more]
XP_011650304.1	2.1e-156	85.00	short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >...	[more]
XP_023552712.1	3.5e-156	83.98	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo ...	[more]
XP_022923250.1	1.0e-155	84.32	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita mosch...	[more]
XP_022965266.1	3.0e-155	83.98	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxim...	[more]

BLAST of Tan0014516.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E6B3	2.6e-157	84.57	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos...	[more]
A0A6J1EB90	4.9e-156	84.32	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos...	[more]
A0A6J1HL83	1.4e-155	83.98	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max...	[more]
A0A1S3BLF4	1.2e-154	83.58	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1CW75	1.6e-154	85.55	short-chain dehydrogenase TIC 32, chloroplastic isoform X1 OS=Momordica charanti...	[more]

BLAST of Tan0014516.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	8.1e-119	68.48	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	2.0e-117	66.27	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	1.1e-80	51.22	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	7.5e-72	47.88	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	7.7e-69	53.28	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 232..249 score: 36.72 coord: 209..228 score: 30.13 coord: 116..127 score: 42.55 coord: 37..54 score: 44.95 coord: 166..182 score: 27.32
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 37..181 e-value: 1.6E-26 score: 93.0
None	No IPR available	GENE3D	3.40.50.720		coord: 23..333 e-value: 3.9E-71 score: 241.5
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..333
None	No IPR available	PANTHER	PTHR24320:SF210	MITOCHONDRIAL PYRUVATE CARRIER	coord: 4..333
None	No IPR available	CDD	cd05327	retinol-DH_like_SDR_c_like	coord: 37..322 e-value: 3.58313E-91 score: 271.405
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 37..301

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0014516	Tan0014516	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0014516.1-exon	Tan0014516.1-exon-LG01:27201085..27201329	exon
Tan0014516.1-exon	Tan0014516.1-exon-LG01:27201413..27201500	exon
Tan0014516.1-exon	Tan0014516.1-exon-LG01:27201578..27201659	exon
Tan0014516.1-exon	Tan0014516.1-exon-LG01:27201774..27201930	exon
Tan0014516.1-exon	Tan0014516.1-exon-LG01:27202010..27202089	exon
Tan0014516.1-exon	Tan0014516.1-exon-LG01:27202199..27202439	exon
Tan0014516.1-exon	Tan0014516.1-exon-LG01:27202518..27202641	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0014516.1-cds	Tan0014516.1-cds-LG01:27201085..27201329	CDS
Tan0014516.1-cds	Tan0014516.1-cds-LG01:27201413..27201500	CDS
Tan0014516.1-cds	Tan0014516.1-cds-LG01:27201578..27201659	CDS
Tan0014516.1-cds	Tan0014516.1-cds-LG01:27201774..27201930	CDS
Tan0014516.1-cds	Tan0014516.1-cds-LG01:27202010..27202089	CDS
Tan0014516.1-cds	Tan0014516.1-cds-LG01:27202199..27202439	CDS
Tan0014516.1-cds	Tan0014516.1-cds-LG01:27202518..27202641	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0014516.1	Tan0014516.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity