Spg038653.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg038653.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	4-coumarate CoA ligase
Location	scaffold12: 6031207 .. 6035376 (-)
Sequence length	1641
RNA-Seq Expression	Spg038653.1
Synteny	Spg038653.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAAAAAAAGTTCATCTGTTCTTCTTCATCTTCATCGGCAAATTTATCGCCATTGAAATGGCCGTGGAAACTCTTCAGAAGGAGTTCATTTTCCGGTCGAAATTGCCGGATATTTACATTCCGAAGCACCTTCCTCTGCATTCGTATTGCCTGCAGGACAATACGGCGAAGATCGGCCACCGGACCTGTCTTGTCAACGGCGTCACAGGCGAATCCTTCACCTACGACGACGTCGATCTCGCCGCTCGCAAGGTCGCCTCCGGATTGAACAAACTCGGCATTGCAAAAGGCGATGTGATCATGCTCCTCCTCCCGAACTCGACGGAATTCGTCTTCGCTTTCCTCGGCGCGTCGTACCGCGGCGCGATCATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCGAAGCAAGCGAAAGGATCGAAGGCGAAATTGATCGTGACGCAAGCGTCGTATTACGAGAAAGTGAAGGATATAACAGAGGAGTTACCTGATGTTAAGATCATGACCGTGGATTCTCCGCCGGACGGTTGTTTGTCGTTCGCCGATTTAATCCAGGCCGACGAAGGCGAGGTTCCGGCGGTGGAGATCCATCCGGACGACGTCGTTGCGCTGCCGTACTCCTCTGGAACCACCGGATTGCCGAAAGGAGTGATGCTAACGCATAAAGGTCTAGTGACGAGTGTGGCTCAGCAAGTGGACGGCGAAAATCCGAATCTGTACTTCCGAAACGAGGATGTGATTTTGTGCGTTTTGCCGCTTTTCCATATCTATTCGCTTAATTCGGTTCTGCTCTGCGGATTGCGAGCCGGTGCGACGATTCTGATTATGCCTAAATTCGAGATCGGTTCGCTTTTGAAATTGGTGGAGAAATACAGAGTTTCGATTGCTCCGATTGTGCCGCCGATCGTTCTCGCCATTGCGAAGTCGCCGGACCTCGATAAGTACGATCTGTCGTCTATCAGAATGATCAAATCCGGCGGAGCTCCGCTCGGTAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCCAAGGCAGTGCTCGGCCAGGTACTGCAAAATCCATAATTTTATTTAATAACTCAGATTTTAAAAAATATATATATATTTATAGAACAGAAAAAAACTTCCCTGCATACTGGTATGTAGGGATCTTTCTACATACCACATTAAAATCTTGACAACTAAGAAAGAGGAAGAGAGAAAATGAGAAATTTTTTTTATCTCTCCTAGTTGTCAACATTTTAATGGTGGTATGCAGGGAAGTTTTTTCCTTATTTATATATTTTTCAACCACCATTTCGTGTGACATATTATAAATTTTGAAATATTGATTGAAATATTAGATATAAAATTGAATTTATTAGAACATATCTTAGATATTTATGGATTTAAAAAAATGCTAGAAATTAAAATTTTAACACATGAAATTGAAAGTTAAATGATTTATTGGACAAATCACATATGAAAATTAGTTCTATTAGATACTTTTTAAAATTCAAAAATCAAATAGACGCAAATCTAAAGTTAGAAACTAAATTAAGGTTTAATTTTTGAGATTAGTAATTATTAATTGTAATTAGGATTCCTTTTAATTCGTATTTAGTCAAAAATTGTTTTTTTCTTTTCTTTTTTTGGGAGGATAATTAGTTGAAATTGTTGATGTGAAGAAAGGCTTGAGACGAAATGACCCCAAGTCAAAGCAGAAGTCCACGTGTAATTTTTTTATTACAAGATACTTTTTTTTACAAAATTAATTACGTTAATTCAATTTTCATTTTCATTTTAAATTTAAAAGTCTATTTTGTGCCACTTGCTAATTTTTTCGAAAGGTTTCGTCCACCTAAACAACAACTCCACGAACCACAAAAGAAAAATCAAGTTGTTATTTTTTTCACCTAAAAACTTTAATCCAAAGTCTTAAATGTTTTTTTTTTTCTTCAAAATAATACATCACAAATATGAGAAGAAAGATTCAAACTTTAACACCTCTTACAAATTTGCAGCTTAGCTTAACTGACATAAAGTTGTATTGTCATTTTCATTTTATTAATACTATAATGTCTTAATAAGTTGAATTAGGTTAATATCGACATCACAACTCTTATACGATTTCCTTTTGGATGTAAGATTAAAATTAAAGTTTAAGCATATTACAACAAATTTGGAAGGTAATAAGTGTATTTTTCAATCAATTATGTTCAAATTAACAAACCTAAAATGTTTGCCAACATGAGCATAGCTGGCATAAGACTTACTAATGACCGAGAGGTCGTTGGTTCGAATCCCCCCACTCCCACATGTTGTCAAAAAAAAAAAAAAAAAAAAAGCCTAAAGTCTTTTAGGTAGCATCACATGAAAATTTCATATTCTAAATCTATATTTAAAAATCAAACCAAATTATTAACTTCAGTATAACTCAACTAATTAAGATATTTGAAATCGAAGTAAAAAAGCATTCAAATAATATTTTTTTCAGACGTGGCACAATCCTTGCCGTCCGTTCATCAAGATTTGATCAGAGAAATTTTTGTTGATTGCAGGGATACGGAATGACGGAGGCCGGTCCAGTGTTAACAATGGGCTTGGCATTTGCAAAGGAACCGTTTCCAATGAAACCAGGCGCCTGCGGCACCGTCGTGAGGAACGCCGAGATGAAGATCGTCGACACCGAAAACGGCGGCTCGTTGCCACGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAGATCATGAAAGGCTATTTGAACAATCCGGAGGCGACGGCCGCCACCATCGACGAGGACGGCTGGTTGCACACCGGCGACATCGGCTTCATCGACGACGACGACGAGCTCTTCATCGTCGATCGGTTGAAGGAGCTGATCAAGTACAAGGGATTCCAAGTGGCTCCGGCGGAACTCGAAGCTCTTCTGCTCACTCATCCGGTCATCTCCGATGCCGCCGTCGTTCCGTAAGAATCAATCGATTCGCTTCGATTTCCGATTGCGATTTGTGAAAATCGCCGTTGATTTTTGTGTGTTTGGTGGTGAATTGCAGAATGAAAGATGAACAAGCTGGAGAGGTTCCCGTTGCATTCGTGGTGAAATTGAAGAACTCTGAAGTTACTGAGGATGAAATTAAACAATTTATTTCCAAACAGGTATGGTTTGAACTAAAAAAAAAAAATATATCTAATCTTATTTTGTTCTCTCAGGCTCCTTTGATAAGTTTTTGGTTTTTGGTTTTTCAAAATTATACTTATTTTATCTCAATTTCCTTACCTTATTTTCATTTTGGTTGAGGAGACATTAGAATTCTTAGTCAAATTCCAAAAACAAAAAAGTTTTTTAAAACTATTTTTTTTTTCCAAATTTTGACTTTTTGAAACACATGGGAAGGAGGTAAGTTCAAACCAAAGAAATGGATAAGTGGAATGAGTGTTTATAAGTTTAATTTTCAAAAACAAAAAAGACAAACAGAGCCTTAATTTTTAATTCTATTCCATTTTTGTCTTAAATATTTATTTCAAACAGTTTTACTCATACTTTCCTAAATTTTTTTTTTTTTTTTTTTTATCATTTTGTTACCATCCATCCACTTAGTAATTGGTCATTAAAGGTAGACTTTAAGATTTAAATATCTTGTCTAAAAGAGAAGAAAAGAAAAAATAATTGAAATCAAAATTGTTTTTTAGGACATATTTTTAAAATTTAAGGACTAAAATAAAATAGATCTAATAATTAAAATTTTATGAAGTAAAATAGAATAATTTGAGAGTAAAATGAGAGTTAAAACTTTTTTTTATTTTTTTTCATATTGGGATTTAGGGATAAGGATATTTTTGAAAAAAGTATATATTTGAAATATTAATTTACAAATTAAAAATGTTAAAAATGATACGTGCAGGTGGTGTTCTACAAGAGAATCAACCGGGTGTTTTTCATAGACGCAATTCCGAAATCACCCTCGGGCAAGATCTTAAGGAAGGAGTTGAGGGCAAAGTTGGCAACTGATTTTCCAAATTCTAATTAATTTTCTTTTTTCTTTTTTTTGCAATTTCACCACACAAACACACACACACACACACCACTAAATGCTAATGCAAGTCTTTTATTTTTCCTCTCTCTCTCTTGTTATTATTACTTTTCTTTTTCTTTATATATATATATATATATATA

mRNA sequence

ATGGCCGTGGAAACTCTTCAGAAGGAGTTCATTTTCCGGTCGAAATTGCCGGATATTTACATTCCGAAGCACCTTCCTCTGCATTCGTATTGCCTGCAGGACAATACGGCGAAGATCGGCCACCGGACCTGTCTTGTCAACGGCGTCACAGGCGAATCCTTCACCTACGACGACGTCGATCTCGCCGCTCGCAAGGTCGCCTCCGGATTGAACAAACTCGGCATTGCAAAAGGCGATGTGATCATGCTCCTCCTCCCGAACTCGACGGAATTCGTCTTCGCTTTCCTCGGCGCGTCGTACCGCGGCGCGATCATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCGAAGCAAGCGAAAGGATCGAAGGCGAAATTGATCGTGACGCAAGCGTCGTATTACGAGAAAGTGAAGGATATAACAGAGGAGTTACCTGATGTTAAGATCATGACCGTGGATTCTCCGCCGGACGGTTGTTTGTCGTTCGCCGATTTAATCCAGGCCGACGAAGGCGAGGTTCCGGCGGTGGAGATCCATCCGGACGACGTCGTTGCGCTGCCGTACTCCTCTGGAACCACCGGATTGCCGAAAGGAGTGATGCTAACGCATAAAGGTCTAGTGACGAGTGTGGCTCAGCAAGTGGACGGCGAAAATCCGAATCTGTACTTCCGAAACGAGGATGTGATTTTGTGCGTTTTGCCGCTTTTCCATATCTATTCGCTTAATTCGGTTCTGCTCTGCGGATTGCGAGCCGGTGCGACGATTCTGATTATGCCTAAATTCGAGATCGGTTCGCTTTTGAAATTGGTGGAGAAATACAGAGTTTCGATTGCTCCGATTGTGCCGCCGATCGTTCTCGCCATTGCGAAGTCGCCGGACCTCGATAAGTACGATCTGTCGTCTATCAGAATGATCAAATCCGGCGGAGCTCCGCTCGGTAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCCAAGGCAGTGCTCGGCCAGGGATACGGAATGACGGAGGCCGGTCCAGTGTTAACAATGGGCTTGGCATTTGCAAAGGAACCGTTTCCAATGAAACCAGGCGCCTGCGGCACCGTCGTGAGGAACGCCGAGATGAAGATCGTCGACACCGAAAACGGCGGCTCGTTGCCACGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAGATCATGAAAGGCTATTTGAACAATCCGGAGGCGACGGCCGCCACCATCGACGAGGACGGCTGGTTGCACACCGGCGACATCGGCTTCATCGACGACGACGACGAGCTCTTCATCGTCGATCGGTTGAAGGAGCTGATCAAGTACAAGGGATTCCAAGTGGCTCCGGCGGAACTCGAAGCTCTTCTGCTCACTCATCCGGTCATCTCCGATGCCGCCGTCGTTCCAATGAAAGATGAACAAGCTGGAGAGGTTCCCGTTGCATTCGTGGTGAAATTGAAGAACTCTGAAGTTACTGAGGATGAAATTAAACAATTTATTTCCAAACAGGTGGTGTTCTACAAGAGAATCAACCGGGTGTTTTTCATAGACGCAATTCCGAAATCACCCTCGGGCAAGATCTTAAGGAAGGAGTTGAGGGCAAAGTTGGCAACTGATTTTCCAAATTCTAATTAA

Coding sequence (CDS)

ATGGCCGTGGAAACTCTTCAGAAGGAGTTCATTTTCCGGTCGAAATTGCCGGATATTTACATTCCGAAGCACCTTCCTCTGCATTCGTATTGCCTGCAGGACAATACGGCGAAGATCGGCCACCGGACCTGTCTTGTCAACGGCGTCACAGGCGAATCCTTCACCTACGACGACGTCGATCTCGCCGCTCGCAAGGTCGCCTCCGGATTGAACAAACTCGGCATTGCAAAAGGCGATGTGATCATGCTCCTCCTCCCGAACTCGACGGAATTCGTCTTCGCTTTCCTCGGCGCGTCGTACCGCGGCGCGATCATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCGAAGCAAGCGAAAGGATCGAAGGCGAAATTGATCGTGACGCAAGCGTCGTATTACGAGAAAGTGAAGGATATAACAGAGGAGTTACCTGATGTTAAGATCATGACCGTGGATTCTCCGCCGGACGGTTGTTTGTCGTTCGCCGATTTAATCCAGGCCGACGAAGGCGAGGTTCCGGCGGTGGAGATCCATCCGGACGACGTCGTTGCGCTGCCGTACTCCTCTGGAACCACCGGATTGCCGAAAGGAGTGATGCTAACGCATAAAGGTCTAGTGACGAGTGTGGCTCAGCAAGTGGACGGCGAAAATCCGAATCTGTACTTCCGAAACGAGGATGTGATTTTGTGCGTTTTGCCGCTTTTCCATATCTATTCGCTTAATTCGGTTCTGCTCTGCGGATTGCGAGCCGGTGCGACGATTCTGATTATGCCTAAATTCGAGATCGGTTCGCTTTTGAAATTGGTGGAGAAATACAGAGTTTCGATTGCTCCGATTGTGCCGCCGATCGTTCTCGCCATTGCGAAGTCGCCGGACCTCGATAAGTACGATCTGTCGTCTATCAGAATGATCAAATCCGGCGGAGCTCCGCTCGGTAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCCAAGGCAGTGCTCGGCCAGGGATACGGAATGACGGAGGCCGGTCCAGTGTTAACAATGGGCTTGGCATTTGCAAAGGAACCGTTTCCAATGAAACCAGGCGCCTGCGGCACCGTCGTGAGGAACGCCGAGATGAAGATCGTCGACACCGAAAACGGCGGCTCGTTGCCACGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAGATCATGAAAGGCTATTTGAACAATCCGGAGGCGACGGCCGCCACCATCGACGAGGACGGCTGGTTGCACACCGGCGACATCGGCTTCATCGACGACGACGACGAGCTCTTCATCGTCGATCGGTTGAAGGAGCTGATCAAGTACAAGGGATTCCAAGTGGCTCCGGCGGAACTCGAAGCTCTTCTGCTCACTCATCCGGTCATCTCCGATGCCGCCGTCGTTCCAATGAAAGATGAACAAGCTGGAGAGGTTCCCGTTGCATTCGTGGTGAAATTGAAGAACTCTGAAGTTACTGAGGATGAAATTAAACAATTTATTTCCAAACAGGTGGTGTTCTACAAGAGAATCAACCGGGTGTTTTTCATAGACGCAATTCCGAAATCACCCTCGGGCAAGATCTTAAGGAAGGAGTTGAGGGCAAAGTTGGCAACTGATTTTCCAAATTCTAATTAA

Protein sequence

MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLATDFPNSN

Homology

[Close]

BLAST of Spg038653.1 vs. NCBI nr
Match: KAG6581611.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.1e-291
Identity = 508/546 (93.04%), Postives = 527/546 (96.52%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MAVE LQ +FIFRSKLPDIYIP HLPLHSYCL +N AKIGHRTCL+N VTGESFTY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIA+GDVIMLLL NS EFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDSPPDGCLSFADLIQADE E+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 DFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Spg038653.1 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 1009.2 bits (2608), Expect = 1.3e-290
Identity = 506/546 (92.67%), Postives = 526/546 (96.34%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MAVE LQ +FIFRSKLPDIYIP HLPLHSYCL +N AKIGHRTCL+N VTGESFTY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIA+GDVIML+L NS EFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDSPPDGCLSFADLIQADE E+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 DFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Spg038653.1 vs. NCBI nr
Match: XP_023526871.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1006.5 bits (2601), Expect = 8.7e-290
Identity = 505/546 (92.49%), Postives = 525/546 (96.15%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MAVE LQ +FIFRSKLP IYIP HLPLHSYCL +N AKIGHRTCL+N VTGESFTY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPHIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIA+GDVIMLLL NS EFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGS AKLIVTQ+SYYEKVK+ITEE+PDVKIMTVDSPPDGCLSFADLIQADE E+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEEVPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVR KFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRTKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 DFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Spg038653.1 vs. NCBI nr
Match: XP_022983944.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.6e-288
Identity = 504/546 (92.31%), Postives = 524/546 (95.97%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MAVE LQ +FIFRSKLPDIYIP HLPLHSYCL +N AKIGHRTCL+N VT ESFTY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIA+GDVI+LLL NS EFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDSPPDGCLSFADLIQADE E+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 DFPNSN 547
            FP SN
Sbjct: 541 GFPISN 546

[Close]

BLAST of Spg038653.1 vs. NCBI nr
Match: XP_023524772.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1000.3 bits (2585), Expect = 6.2e-288
Identity = 506/546 (92.67%), Postives = 524/546 (95.97%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MA ET+  +FIFRSKLPDIYIPKHLPLHSYCLQ+  A+IGHRTCL+N VTGESFTY DVD
Sbjct: 1   MATETVH-DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARK ASGL KLGIAKGDVIMLLLPNS EFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGSKAKLI+TQ+SYYEKVK+ITE+LPD KIMTVDSPP GCLSFADLIQ  + E+PAVEI
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGATILIMPKFEIGSLL+LVEKYRVSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAE+KIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 DFPNSN 547
           DFP  N
Sbjct: 541 DFPIPN 545

[Close]

BLAST of Spg038653.1 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 2.0e-233
Identity = 409/544 (75.18%), Postives = 458/544 (84.19%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           M  +T Q + IFRSKLPDIYIP HLPLHSYC  +N ++   R CL+NG   + +TY DV+
Sbjct: 1   MEKDTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVE 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L +RKVA+GL+K GI   D IM+LLPNS EFVFAF+GASY GAI T ANP FT AE+ KQ
Sbjct: 61  LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AK S AK+IVTQA +  KVKD   E  DVKI+ +DS P+GCL F+ L QA+E ++P VEI
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY  +EDV+LCVLPLFHI
Sbjct: 181 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLR GA ILIM KF+I S L+L+++Y+V+I P VPPIVLAIAKSP +D YD
Sbjct: 241 YSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSS+R + SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K G
Sbjct: 301 LSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAEMKIVD + G SLPRN  GEICIRGDQIMKGYLN+PEATA TID++GWL+T
Sbjct: 361 ACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG+IDDDDELFIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+   S +TEDE+K FISKQV+FYKRI RVFF+DAIPKSPSGKILRK+LRAKLA 
Sbjct: 481 PVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAA 540

Query: 541 DFPN 545
             PN
Sbjct: 541 GLPN 542

[Close]

BLAST of Spg038653.1 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 4.5e-233
Identity = 402/538 (74.72%), Postives = 462/538 (85.87%), Query Frame = 0


Query: 7   QKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLAARKV 66
           +++ IFRSKLPDIYIPKHLPLH+YC  +N +K+G ++CL+NG TGE+FTY  V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  ASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKA 126
           ASGLNKLGI +GD IMLLLPNS E+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S+A
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQA 127

Query: 127 KLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEIHPDDVV 186
           KLI+TQA Y +KVKD   E  +++I+ +D  P  CL F+ L++ADE E+P V I+ DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSIRM 306
           L CGLRAG TILIM KF+I   L+L++KY+V+I P VPPIVLAIAKSP +DKYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 367 RNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIGFI 426
           RNAEMKIVD E   SLPRN  GEICIRGDQIMKGYLN+PE+T  TIDE+GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 427 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
           DDDDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 487 KLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT-DFP 544
           +      TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRA++A+ D P
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASGDLP 543

[Close]

BLAST of Spg038653.1 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 7.8e-233
Identity = 403/538 (74.91%), Postives = 460/538 (85.50%), Query Frame = 0


Query: 7   QKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLAARKV 66
           +++ IFRSKLPDIYIPKHLPLH+YC  +N +K+G ++CL+NG TGE+FTY  V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  ASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKA 126
           ASGLNKLGI +GD IMLLLPNS E+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S A
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA 127

Query: 127 KLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEIHPDDVV 186
           KLI+TQA Y +KVKD   E  +++I+ +D  P  CL F+ L++ADE E+P V I  DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSIRM 306
           L CGLRAG TILIM KF+I   L+L++KY+V+I P VPPIVLAIAKSP +DKYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 367 RNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIGFI 426
           RNAEMKIVD E   SLPRN  GEICIRGDQIMKGYLN+PE+T  TIDE+GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 427 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
           DDDDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 487 KLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT-DFP 544
           +      TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRAK+A+ D P
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASGDLP 543

[Close]

BLAST of Spg038653.1 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 6.6e-232
Identity = 401/537 (74.67%), Postives = 458/537 (85.29%), Query Frame = 0


Query: 7   QKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLAARKV 66
           Q+EFIFRSKLPDIYIP HLPLHSYC  +N ++   R CL+NG TGE  TY DVDL +RKV
Sbjct: 10  QEEFIFRSKLPDIYIPNHLPLHSYCF-ENISQFKDRPCLINGATGEIITYADVDLTSRKV 69

Query: 67  ASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKA 126
           A+GL+KLGI +GDVIMLLL NS EFV+AFL ASY GAI+T ANPF+T AE+AKQA  SK 
Sbjct: 70  AAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASKT 129

Query: 127 KLIVTQASYYEKVKDITEELPDVKIMTVDSPP--DGCLSFADLIQADEGEVPAVEIHPDD 186
           KL++T A Y +KVK+ T     VK+M VD+PP    CL F++L QADE E+PAV+IHPDD
Sbjct: 130 KLVITLAGYIDKVKEFTGGESGVKVMCVDAPPPESECLHFSELTQADETEIPAVKIHPDD 189

Query: 187 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHIYSLN 246
           VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLYF   DVILCVLPLFHIYSLN
Sbjct: 190 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHIYSLN 249

Query: 247 SVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSI 306
           S+LLCGLR GA ILIM KFEI  LL+L+EK++V+IAP VPPIVL++AK PDL +YDLSSI
Sbjct: 250 SILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYDLSSI 309

Query: 307 RMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGT 366
           R + SGGAP+GKELED V+ K P A LGQGYGMTEAGPVL+M LAFAKEPFP+K GACGT
Sbjct: 310 RTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSGACGT 369

Query: 367 VVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIG 426
           VVRNAEMKIVD + G SLPRN  GEICIRG QIMKGY+N+ EAT  TIDE GWLHTGDIG
Sbjct: 370 VVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHTGDIG 429

Query: 427 FIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAF 486
           FID+DDELFIVDRLKELIKYKGFQVAPAELE++L++HP I+DAAVVPMKDE AGEVPVAF
Sbjct: 430 FIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEVPVAF 489

Query: 487 VVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLATD 542
           VV+   S++TE++IKQ+ISKQVVFYKRIN+ FFI+ IPK+PSGKILRK LRAKL T+
Sbjct: 490 VVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAKLVTE 545

[Close]

BLAST of Spg038653.1 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 1.5e-231
Identity = 399/542 (73.62%), Postives = 454/542 (83.76%), Query Frame = 0


Query: 4   ETLQK-EFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLA 63
           ET Q  + IFRSKLPDIYIPKHLPLHSYC  +N ++   R CL+NG   + +TY +V+L 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELT 67

Query: 64  ARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAK 123
            RKVA GLNKLGI + D IM+LLPNS EFVFAF+GASY GAI T ANP FT AE+ KQAK
Sbjct: 68  CRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAK 127

Query: 124 GSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEIHP 183
            S AK+I+TQ+ +  KVKD   E  DVK++ +DS P+GCL F++L Q+DE E+P V+I P
Sbjct: 128 ASSAKIIITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQP 187

Query: 184 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHIYS 243
           DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NLY  +EDV++CVLPLFHIYS
Sbjct: 188 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYS 247

Query: 244 LNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLS 303
           LNS+LLCGLR GA ILIM KF+I   L+L++KY+VSI P VPPIVLAIAKSP +D YDLS
Sbjct: 248 LNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLS 307

Query: 304 SIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGAC 363
           S+R + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GAC
Sbjct: 308 SVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 367

Query: 364 GTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGD 423
           GTVVRNAEMKIVD + G SLPRN PGEICIRGDQIMKGYLN+PEAT  TID++GWLHTGD
Sbjct: 368 GTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGD 427

Query: 424 IGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPV 483
           IGFID+DDELFIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDEQAGEVPV
Sbjct: 428 IGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPV 487

Query: 484 AFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLATDF 543
           AFVV+   S +TEDE+K FISKQV+FYKR+ RVFF++ +PKSPSGKILRK+LRA+LA   
Sbjct: 488 AFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGV 547

Query: 544 PN 545
           PN
Sbjct: 548 PN 547

[Close]

BLAST of Spg038653.1 vs. ExPASy TrEMBL
Match: A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 6.5e-291
Identity = 506/546 (92.67%), Postives = 526/546 (96.34%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MAVE LQ +FIFRSKLPDIYIP HLPLHSYCL +N AKIGHRTCL+N VTGESFTY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIA+GDVIML+L NS EFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDSPPDGCLSFADLIQADE E+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 DFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Spg038653.1 vs. ExPASy TrEMBL
Match: A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 1.8e-288
Identity = 504/546 (92.31%), Postives = 524/546 (95.97%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MAVE LQ +FIFRSKLPDIYIP HLPLHSYCL +N AKIGHRTCL+N VT ESFTY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARKVASGLNKLGIA+GDVI+LLL NS EFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDSPPDGCLSFADLIQADE E+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 DFPNSN 547
            FP SN
Sbjct: 541 GFPISN 546

[Close]

BLAST of Spg038653.1 vs. ExPASy TrEMBL
Match: A0A6J1G9T7 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 4.8e-286
Identity = 503/543 (92.63%), Postives = 521/543 (95.95%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MA ET+  +FIFRSKLPDIYIPKHLPLHSYCLQ+  A+IGHRTCL+N VTGESFTY DVD
Sbjct: 1   MATETVH-DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARK ASGL KLGIAKGDVIMLLLPNS EFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGSKAKLI+TQ+SYYEKVK+ITE+L D KIMTVDSP  GCLSFADLIQ  + ++PAVEI
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGATILIMPKFEIGSLL+LVEKYRVSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAE+KIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 DFP 544
           DFP
Sbjct: 541 DFP 542

[Close]

BLAST of Spg038653.1 vs. ExPASy TrEMBL
Match: A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.4e-285
Identity = 502/546 (91.94%), Postives = 521/546 (95.42%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           MA ET+  + IFRSKLPDIYIPKHLPLHSYCLQ+  A+IGHRTCL+N VTGESFTY DVD
Sbjct: 1   MANETVH-DIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LAARK ASGL KLGIAKGDVIMLLLPNS EFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGSKAKL +TQ+SYYEKVK+ITE+LPD KIMTVDSPP GCLSFADLIQ  + E+PAVEI
Sbjct: 121 AKGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGATILIMPKFEIGSLL+LVEKYRVSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAE+KIVDTENGGSLPRNTPGEICI+GDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVKLKNS+VTEDEIKQFISKQVVFYKRINRVFFI AIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVKLKNSKVTEDEIKQFISKQVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLAA 540

Query: 541 DFPNSN 547
           DFP  N
Sbjct: 541 DFPIPN 545

[Close]

BLAST of Spg038653.1 vs. ExPASy TrEMBL
Match: A0A5A7SWF8 (4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00110 PE=4 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 4.5e-284
Identity = 494/546 (90.48%), Postives = 521/546 (95.42%), Query Frame = 0


Query: 1   MAVETLQKEFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVD 60
           M +ET++ + IFRSKLPDIYIP HLPLHSYCLQ+N AKIGHRTCL+NGVTGESFT++DVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LAARKVASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGI K DVIMLLLPNS EFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSPPDGCLSFADLIQADEGEVPAVEI 180
           AKGSKAKLI+TQ+SYYEK+K+ITEELP+VKIMTVDSP DGCL F DLI+ADE E+P VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ NEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LL+LVEKY V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
           PVAFVVKLKNSE TEDEIKQFISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 DFPNSN 547
            FPNSN
Sbjct: 541 AFPNSN 546

[Close]

BLAST of Spg038653.1 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 759.6 bits (1960), Expect = 1.7e-219
Identity = 377/540 (69.81%), Postives = 455/540 (84.26%), Query Frame = 0


Query: 9   EFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLAARKVAS 68
           + IFRS+LPDIYIP HLPLH Y + +N ++   + CL+NG TGE +TY DV + +RK+A+
Sbjct: 20  DVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAA 79

Query: 69  GLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 128
           GL+ LG+ + DV+M+LLPNS E V  FL AS+ GAI T+ANPFFT AEI+KQAK S AKL
Sbjct: 80  GLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKL 139

Query: 129 IVTQASYYEKVKDITEELPDVKIMTVDSP--PDGCLSFADLIQADEGEVPAV--EIHPDD 188
           IVTQ+ Y +K+K++  +   V I+T DS   P+ CL F++L Q++E  V ++  +I P+D
Sbjct: 140 IVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPED 199

Query: 189 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHIYSLN 248
           VVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF  +DVILCVLP+FHIY+LN
Sbjct: 200 VVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALN 259

Query: 249 SVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSI 308
           S++LC LR GATILIMPKFEI  LL+ +++ +V++A +VPPIVLAIAKSP+ +KYDLSS+
Sbjct: 260 SIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSV 319

Query: 309 RMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGT 368
           RM+KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACGT
Sbjct: 320 RMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGT 379

Query: 369 VVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIG 428
           VVRNAEMKI+D + G SLPRN PGEICIRG+QIMKGYLN+P ATA+TID+DGWLHTGD+G
Sbjct: 380 VVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVG 439

Query: 429 FIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAF 488
           FIDDDDELFIVDRLKELIKYKGFQVAPAELE+LL+ HP I+D AVV MK+E AGEVPVAF
Sbjct: 440 FIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAF 499

Query: 489 VVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLATDFPN 545
           VV+ K+S ++EDEIKQF+SKQVVFYKRIN+VFF D+IPK+PSGKILRK+LRA+LA    N
Sbjct: 500 VVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

[Close]

BLAST of Spg038653.1 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 756.1 bits (1951), Expect = 1.9e-218
Identity = 382/539 (70.87%), Postives = 447/539 (82.93%), Query Frame = 0


Query: 9   EFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLAARKVAS 68
           + IFRSKLPDIYIP HL LH Y  Q N ++   + CL+NG TG  +TY DV + +R++A+
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIAA 81

Query: 69  GLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 128
             +KLG+ + DV+MLLLPN  EFV +FL AS+RGA  TAANPFFT AEIAKQAK S  KL
Sbjct: 82  NFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKL 141

Query: 129 IVTQASYYEKVKDITEELPDVKIMTVDSP-----PDGCLSFADLIQA--DEGEV-PAVEI 188
           I+T+A Y +K+K +  +   V I+ +D       P+GCL F +L Q+  +  EV  +VEI
Sbjct: 142 IITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 201

Query: 189 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 248
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF ++DVILCVLP+FHI
Sbjct: 202 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 261

Query: 249 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 308
           Y+LNS++LCGLR GA ILIMPKFEI  LL+L+++ +V++AP+VPPIVLAIAKS + +KYD
Sbjct: 262 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 321

Query: 309 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 368
           LSSIR++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K G
Sbjct: 322 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 381

Query: 369 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 428
           ACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP ATA TID+DGWLHT
Sbjct: 382 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 441

Query: 429 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 488
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 442 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 501

Query: 489 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVVK K+SE++ED++KQF+SKQVVFYKRIN+VFF ++IPK+PSGKILRK+LRAKLA
Sbjct: 502 PVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

[Close]

BLAST of Spg038653.1 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 701.0 bits (1808), Expect = 7.3e-202
Identity = 355/506 (70.16%), Postives = 415/506 (82.02%), Query Frame = 0


Query: 9   EFIFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLAARKVAS 68
           + IFRSKLPDIYIP HL LH Y  Q N ++   + CL+NG TG  +TY DV + +R++A+
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIAA 81

Query: 69  GLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 128
             +KLG+ + DV+MLLLPN  EFV +FL AS+RGA  TAANPFFT AEIAKQAK S  KL
Sbjct: 82  NFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKL 141

Query: 129 IVTQASYYEKVKDITEELPDVKIMTVDSP-----PDGCLSFADLIQA--DEGEV-PAVEI 188
           I+T+A Y +K+K +  +   V I+ +D       P+GCL F +L Q+  +  EV  +VEI
Sbjct: 142 IITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 201

Query: 189 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHI 248
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF ++DVILCVLP+FHI
Sbjct: 202 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 261

Query: 249 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 308
           Y+LNS++LCGLR GA ILIMPKFEI  LL+L+++ +V++AP+VPPIVLAIAKS + +KYD
Sbjct: 262 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 321

Query: 309 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 368
           LSSIR++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K G
Sbjct: 322 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 381

Query: 369 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 428
           ACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP ATA TID+DGWLHT
Sbjct: 382 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 441

Query: 429 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 488
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 442 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 501

Query: 489 PVAFVVKLKNSEVTEDEIKQFISKQV 507
           PVAFVVK K+SE++ED++KQF+SKQV
Sbjct: 502 PVAFVVKSKDSELSEDDVKQFVSKQV 525

[Close]

BLAST of Spg038653.1 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 691.8 bits (1784), Expect = 4.4e-199
Identity = 340/531 (64.03%), Postives = 432/531 (81.36%), Query Frame = 0


Query: 11  IFRSKLPDIYIPKHLPLHSYCLQDNTAKIGHRTCLVNGVTGESFTYDDVDLAARKVASGL 70
           IFRSKLPDI IP HLPLH+YC  +  + +  + CL+ G TG+S+TY +  L  R+VASGL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 71  NKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKLIV 130
            KLGI KGDVIM+LL NS EFVF+F+GAS  GA+ T ANPF+T+ E+ KQ K S AKLI+
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 131 TQASYYEKVKDITEELPDVKIMTVDSP-PDGCLSFADLIQADEGE--VPAVEIHPDDVVA 190
           T + Y +K+K++ E L    ++T D P P+ CL F+ LI  DE       V+I  DD  A
Sbjct: 154 THSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 191 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLPLFHIYSLNSVL 250
           LP+SSGTTGLPKGV+LTHK L+TSVAQQVDG+NPNLY ++ DVILCVLPLFHIYSLNSVL
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 251 LCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSIRMI 310
           L  LR+GAT+L+M KFEIG+LL L++++RV+IA +VPP+V+A+AK+P ++ YDLSS+R +
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 311 KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVR 370
            SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CGTVVR
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 371 NAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIGFID 430
           NAE+K+V  E   SL  N PGEICIRG QIMK YLN+PEAT+ATIDE+GWLHTGDIG++D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 431 DDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVK 490
           +DDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I+DAAVVP  DE AGEVPVAFVV+
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 491 LKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 539
              +++TE+++K++++KQVVFYKR+++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

[Close]

BLAST of Spg038653.1 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 668.3 bits (1723), Expect = 5.2e-192
Identity = 330/544 (60.66%), Postives = 420/544 (77.21%), Query Frame = 0


Query: 9   EFIFRSKLPDIYIPKHLPLHSYCLQDNTAK---IGHRTCLVNGVTGESFTYDDVDLAARK 68
           +FIFRSKLPDI+IP HLPL  Y  Q  +         TC+++G TG   TY DV    R+
Sbjct: 26  DFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNMRR 85

Query: 69  VASGLNKLGIAKGDVIMLLLPNSTEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSK 128
           +A+G+++LGI  GDV+MLLLPNS EF  +FL  +Y GA+ T ANPF+T  EIAKQAK S 
Sbjct: 86  IAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKASA 145

Query: 129 AKLIVTQASYYEKVKDITEELPDVKIMTVD---------SPPDGCLSFADLIQADEGEVP 188
           AK+I+T+    +K+ ++  +   V I+ +D         S  DGC+SF +L QADE E+ 
Sbjct: 146 AKMIITKKCLVDKLTNLKND--GVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETELL 205

Query: 189 AVEIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNEDVILCVLP 248
             +I P+D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDGENPNL F   DVILC LP
Sbjct: 206 KPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLP 265

Query: 249 LFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDL 308
           +FHIY+L++++L  +R GA +LI+P+FE+  +++L+++Y+V++ P+ PP+VLA  KSP+ 
Sbjct: 266 MFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSPET 325

Query: 309 DKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFP 368
           ++YDLSS+R++ SG A L KELED VR KFP A+ GQGYGMTE+G V    LAFAK PF 
Sbjct: 326 ERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNPFK 385

Query: 369 MKPGACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDG 428
            K GACGTV+RNAEMK+VDTE G SLPRN  GEIC+RG Q+MKGYLN+PEATA TID+DG
Sbjct: 386 TKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDKDG 445

Query: 429 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQ 488
           WLHTGDIGF+DDDDE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV MKDE 
Sbjct: 446 WLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDEV 505

Query: 489 AGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRA 541
           A EVPVAFV + + S++TED++K +++KQVV YKRI  VFFI+ IPK+ SGKILRK+LRA
Sbjct: 506 ADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDLRA 565

The following BLAST results are available for this feature:

BLAST of Spg038653.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581611.1	2.1e-291	93.04	4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022935389.1	1.3e-290	92.67	4-coumarate--CoA ligase 1-like [Cucurbita moschata]	[more]
XP_023526871.1	8.7e-290	92.49	4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022983944.1	3.6e-288	92.31	4-coumarate--CoA ligase 1-like [Cucurbita maxima]	[more]
XP_023524772.1	6.2e-288	92.67	4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg038653.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24146	2.0e-233	75.18	4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1	[more]
P14912	4.5e-233	74.72	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1	[more]
P14913	7.8e-233	74.91	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1	[more]
M4ISH0	6.6e-232	74.67	4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1	[more]
O24145	1.5e-231	73.62	4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1	[more]

BLAST of Spg038653.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F599	6.5e-291	92.67	4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=...	[more]
A0A6J1J912	1.8e-288	92.31	4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 ...	[more]
A0A6J1G9T7	4.8e-286	92.63	4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KCG9	2.4e-285	91.94	4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 ...	[more]
A0A5A7SWF8	4.5e-284	90.48	4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Spg038653.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21240.1	1.7e-219	69.81	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	1.9e-218	70.87	4-coumarate:CoA ligase 1	[more]
AT1G51680.3	7.3e-202	70.16	4-coumarate:CoA ligase 1	[more]
AT1G65060.1	4.4e-199	64.03	4-coumarate:CoA ligase 3	[more]
AT3G21230.1	5.2e-192	60.66	4-coumarate:CoA ligase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.300.30		coord: 439..541 e-value: 1.2E-32 score: 114.7
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 6..540
None	No IPR available	PANTHER	PTHR24096:SF382	4-COUMARATE--COA LIGASE 2-LIKE	coord: 6..540
None	No IPR available	CDD	cd05904	4CL	coord: 21..534 e-value: 0.0 score: 782.19
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 20..540
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 5..438 e-value: 6.1E-173 score: 577.3
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 34..444 e-value: 4.4E-111 score: 371.5
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 453..528 e-value: 2.3E-16 score: 60.5
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 188..199

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg038653	Spg038653	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg038653.1-three_prime_utr	Spg038653.1-three_prime_utr-scaffold12:6031207..6031352	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg038653.1-cds	Spg038653.1-cds-scaffold12:6031353..6031478	CDS
Spg038653.1-cds	Spg038653.1-cds-scaffold12:6032225..6032327	CDS
Spg038653.1-cds	Spg038653.1-cds-scaffold12:6032414..6032826	CDS
Spg038653.1-cds	Spg038653.1-cds-scaffold12:6034320..6035318	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg038653.1-five_prime_utr	Spg038653.1-five_prime_utr-scaffold12:6035319..6035376	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg038653.1	Spg038653.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016405	CoA-ligase activity