Spg025302.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg025302.1 vs. NCBI nr
Match: XP_016899921.1 (PREDICTED: uncharacterized protein LOC107990705 [Cucumis melo])

HSP 1 Score: 167.2 bits (422), Expect = 1.8e-37
Identity = 98/179 (54.75%), Postives = 127/179 (70.95%), Query Frame = 0

Query: 65  KEASVLSIET-------ETGNGKNVSSKDG---DRDFLMVITTFIATMAFQAGINPPGGV 124
           ++ SVLSIET       +  N K    K+G   DRDFLMV TTFIA MAFQAG NPPGGV
Sbjct: 6   EDESVLSIETDQTINEKDKENQKKKKKKEGEGRDRDFLMVATTFIAAMAFQAGTNPPGGV 65

Query: 125 WDDDDK-GHVAGKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTF 184
           W DD+   + AGKSIMATKSP+ FI F++GVTVC + SAMQ +VLL  S++   SVSK+F
Sbjct: 66  WQDDNSPNYEAGKSIMATKSPSLFISFMSGVTVCFACSAMQFIVLLGKSSEKSWSVSKSF 125

Query: 185 MFFSIGSTISFMAVAYWNSVTALTPESEMSKVKPMFWFTIGFS-VLLPILVYLSKKLLS 232
           ++F++GS IS + VAYW+++ ALTPE++MS V  M   T+  S V+LPI +Y+ KK L+
Sbjct: 126 IYFTMGSGISAIVVAYWSAIKALTPEAQMSHVIVMLKTTLVLSVVVLPISMYIEKKRLA 184

BLAST of Spg025302.1 vs. NCBI nr
Match: KAA0064719.1 (ankyrin repeat-containing protein-like [Cucumis melo var. makuwa] >TYK00739.1 ankyrin repeat-containing protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-32
Identity = 82/141 (58.16%), Postives = 107/141 (75.89%), Query Frame = 0

Query: 93  MVITTFIATMAFQAGINPPGGVWDDDDK-GHVAGKSIMATKSPNQFILFITGVTVCLSVS 152
           MV TTFIA MAFQAG NPPGGVW DD+   + AGKSIMATKSP+ FI F++GVTVC + S
Sbjct: 1   MVATTFIAAMAFQAGTNPPGGVWQDDNSPNYEAGKSIMATKSPSLFISFMSGVTVCFACS 60

Query: 153 AMQLVVLLVNSTDNRRSVSKTFMFFSIGSTISFMAVAYWNSVTALTPESEMSKVKPMFWF 212
           AMQ +VLL  S++   SVSK+F++F++GS IS + VAYW+++ ALTPE++MS V  M   
Sbjct: 61  AMQFIVLLGKSSEKSWSVSKSFIYFTMGSGISAIVVAYWSAIKALTPEAQMSHVIVMLKT 120

Query: 213 TIGFS-VLLPILVYLSKKLLS 232
           T+  S V+LPI +Y+ KK L+
Sbjct: 121 TLVLSVVVLPISMYIEKKRLA 141

BLAST of Spg025302.1 vs. NCBI nr
Match: XP_038884181.1 (uncharacterized protein LOC120075089 [Benincasa hispida])

HSP 1 Score: 143.3 bits (360), Expect = 2.8e-30
Identity = 84/178 (47.19%), Postives = 119/178 (66.85%), Query Frame = 0

Query: 65  KEASVLSIETETGNGK-----NVSSKDGDRDFLMVITTFIATMAFQAGINPPGGVWDDDD 124
           +EA+ +SI+TE  + K     N    + D DF+MVI TFIAT+AFQAG NPPGGVW +D 
Sbjct: 10  EEATAVSIDTERESRKDREEANQGWIERDVDFVMVIVTFIATVAFQAGTNPPGGVWQEDK 69

Query: 125 K----GHVAGKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTFMF 184
           K     ++AGKSIM TKSP+++I F+ GVTVCL+ S +QL+V+L N      S+ +  ++
Sbjct: 70  KVNGTDYIAGKSIMGTKSPSEYIKFMVGVTVCLAFSVIQLIVMLFNWYLKSWSIRRAILY 129

Query: 185 FSIGSTISFMAVAYWNSVTALTPESEMSKVKPMFWFTIGFS-VLLP--ILVYLSKKLL 231
             +  TI+ M VA+W+S+ ALTP   M+++K  FW  IG S ++LP  +L YL+KK L
Sbjct: 130 MLMLLTITPMVVAFWSSLLALTPADLMTEIKAGFWSVIGVSLIVLPFVLLYYLTKKCL 187

BLAST of Spg025302.1 vs. NCBI nr
Match: KAE8647228.1 (hypothetical protein Csa_018955 [Cucumis sativus])

HSP 1 Score: 132.5 bits (332), Expect = 4.9e-27
Identity = 93/238 (39.08%), Postives = 133/238 (55.88%), Query Frame = 0

Query: 5   IFPLHSFLKSFCGPQLKAAVCGKDEEGQVA--TLGDGQNKDNGSQLVSRRGRFPSSSSTM 64
           ++PL +  +   G +L     G  E  Q++  T  D  N D    L +       S S  
Sbjct: 137 VYPLETGSQEQYGDELPR---GDRENKQISSQTQDDSTNTDQSLNL-NTHPMVTRSKSGA 196

Query: 65  SAKEASVLSIETETGNGKNVSSKD------GDRDFLMVITTFIATMAFQAGINPPGGVWD 124
             +EA+V+SI+T + N K+   K+      GD DF+MV+ TFIA +AFQ GINPPG VW 
Sbjct: 197 KNEEATVVSIDTASENKKDREEKNSGWMKPGDVDFVMVVVTFIAAVAFQVGINPPGSVWQ 256

Query: 125 DDDKGHVAGKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTFMFF 184
           +D  G  AGKSIMA+KSP+++  F+ GVT+CL  S +QL V+L        SV +  M+ 
Sbjct: 257 EDKNGFTAGKSIMASKSPSEYKKFMAGVTLCLGFSLIQLCVMLFRWYLKSYSVRRMIMYI 316

Query: 185 SIGSTISFMAVAYWNSVTALTPESE-MSKVKPMFWFTIG-FSVLLPI--LVYLSKKLL 231
            +  TI+ M V++W SV ALTP+ + MS++    W  +G + + LPI  L YL K  L
Sbjct: 317 LMLCTIAPMIVSFWASVKALTPDEKLMSEITATIWSILGVYLISLPIILLYYLVKTFL 370

BLAST of Spg025302.1 vs. NCBI nr
Match: XP_004144204.1 (uncharacterized protein LOC101208403 [Cucumis sativus])

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-26
Identity = 79/176 (44.89%), Postives = 111/176 (63.07%), Query Frame = 0

Query: 65  KEASVLSIETETGNGKNVSSKD------GDRDFLMVITTFIATMAFQAGINPPGGVWDDD 124
           +EA+V+SI+T + N K+   K+      GD DF+MV+ TFIA +AFQ GINPPG VW +D
Sbjct: 9   EEATVVSIDTASENKKDREEKNSGWMKPGDVDFVMVVVTFIAAVAFQVGINPPGSVWQED 68

Query: 125 DKGHVAGKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTFMFFSI 184
             G  AGKSIMA+KSP+++  F+ GVT+CL  S +QL V+L        SV +  M+  +
Sbjct: 69  KNGFTAGKSIMASKSPSEYKKFMAGVTLCLGFSLIQLCVMLFRWYLKSYSVRRMIMYILM 128

Query: 185 GSTISFMAVAYWNSVTALTPESE-MSKVKPMFWFTIG-FSVLLPI--LVYLSKKLL 231
             TI+ M V++W SV ALTP+ + MS++    W  +G + + LPI  L YL K  L
Sbjct: 129 LCTIAPMIVSFWASVKALTPDEKLMSEITATIWSILGVYLISLPIILLYYLVKTFL 184

BLAST of Spg025302.1 vs. ExPASy TrEMBL
Match: A0A1S4DVA8 (uncharacterized protein LOC107990705 OS=Cucumis melo OX=3656 GN=LOC107990705 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 8.6e-38
Identity = 98/179 (54.75%), Postives = 127/179 (70.95%), Query Frame = 0

Query: 65  KEASVLSIET-------ETGNGKNVSSKDG---DRDFLMVITTFIATMAFQAGINPPGGV 124
           ++ SVLSIET       +  N K    K+G   DRDFLMV TTFIA MAFQAG NPPGGV
Sbjct: 6   EDESVLSIETDQTINEKDKENQKKKKKKEGEGRDRDFLMVATTFIAAMAFQAGTNPPGGV 65

Query: 125 WDDDDK-GHVAGKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTF 184
           W DD+   + AGKSIMATKSP+ FI F++GVTVC + SAMQ +VLL  S++   SVSK+F
Sbjct: 66  WQDDNSPNYEAGKSIMATKSPSLFISFMSGVTVCFACSAMQFIVLLGKSSEKSWSVSKSF 125

Query: 185 MFFSIGSTISFMAVAYWNSVTALTPESEMSKVKPMFWFTIGFS-VLLPILVYLSKKLLS 232
           ++F++GS IS + VAYW+++ ALTPE++MS V  M   T+  S V+LPI +Y+ KK L+
Sbjct: 126 IYFTMGSGISAIVVAYWSAIKALTPEAQMSHVIVMLKTTLVLSVVVLPISMYIEKKRLA 184

BLAST of Spg025302.1 vs. ExPASy TrEMBL
Match: A0A5D3BRK9 (Ankyrin repeat-containing protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00290 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 8.4e-33
Identity = 82/141 (58.16%), Postives = 107/141 (75.89%), Query Frame = 0

Query: 93  MVITTFIATMAFQAGINPPGGVWDDDDK-GHVAGKSIMATKSPNQFILFITGVTVCLSVS 152
           MV TTFIA MAFQAG NPPGGVW DD+   + AGKSIMATKSP+ FI F++GVTVC + S
Sbjct: 1   MVATTFIAAMAFQAGTNPPGGVWQDDNSPNYEAGKSIMATKSPSLFISFMSGVTVCFACS 60

Query: 153 AMQLVVLLVNSTDNRRSVSKTFMFFSIGSTISFMAVAYWNSVTALTPESEMSKVKPMFWF 212
           AMQ +VLL  S++   SVSK+F++F++GS IS + VAYW+++ ALTPE++MS V  M   
Sbjct: 61  AMQFIVLLGKSSEKSWSVSKSFIYFTMGSGISAIVVAYWSAIKALTPEAQMSHVIVMLKT 120

Query: 213 TIGFS-VLLPILVYLSKKLLS 232
           T+  S V+LPI +Y+ KK L+
Sbjct: 121 TLVLSVVVLPISMYIEKKRLA 141

BLAST of Spg025302.1 vs. ExPASy TrEMBL
Match: A0A0A0KIL3 (PGG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G363520 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 5.2e-27
Identity = 79/176 (44.89%), Postives = 111/176 (63.07%), Query Frame = 0

Query: 65  KEASVLSIETETGNGKNVSSKD------GDRDFLMVITTFIATMAFQAGINPPGGVWDDD 124
           +EA+V+SI+T + N K+   K+      GD DF+MV+ TFIA +AFQ GINPPG VW +D
Sbjct: 9   EEATVVSIDTASENKKDREEKNSGWMKPGDVDFVMVVVTFIAAVAFQVGINPPGSVWQED 68

Query: 125 DKGHVAGKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTFMFFSI 184
             G  AGKSIMA+KSP+++  F+ GVT+CL  S +QL V+L        SV +  M+  +
Sbjct: 69  KNGFTAGKSIMASKSPSEYKKFMAGVTLCLGFSLIQLCVMLFRWYLKSYSVRRMIMYILM 128

Query: 185 GSTISFMAVAYWNSVTALTPESE-MSKVKPMFWFTIG-FSVLLPI--LVYLSKKLL 231
             TI+ M V++W SV ALTP+ + MS++    W  +G + + LPI  L YL K  L
Sbjct: 129 LCTIAPMIVSFWASVKALTPDEKLMSEITATIWSILGVYLISLPIILLYYLVKTFL 184

BLAST of Spg025302.1 vs. ExPASy TrEMBL
Match: A0A1S3BCY4 (uncharacterized protein LOC103488512 OS=Cucumis melo OX=3656 GN=LOC103488512 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 5.2e-27
Identity = 78/176 (44.32%), Postives = 113/176 (64.20%), Query Frame = 0

Query: 65  KEASVLSIETETGN---GKNVSS---KDGDRDFLMVITTFIATMAFQAGINPPGGVWDDD 124
           +EA+V++I+TET N   G+ ++S   + GD DF+M++ TFIA +AFQ G NPPG VW +D
Sbjct: 9   EEATVVAIDTETENRKDGEEMNSGWMRPGDVDFVMIVVTFIAAVAFQVGTNPPGSVWQED 68

Query: 125 DKGHVAGKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTFMFFSI 184
             G  AGKSIMA+KSP+++  F+ GVT+CLS S +QL V+L        SV +  M+  +
Sbjct: 69  KNGFTAGKSIMASKSPSEYKKFMAGVTLCLSFSLIQLSVMLFRWYLKTYSVRRMIMYLLM 128

Query: 185 GSTISFMAVAYWNSVTALTPESE-MSKVKPMFWFTIG---FSVLLPILVYLSKKLL 231
             TI+ M  ++W SV ALTP+ + MS++    W + G    S+ L +L YL KK L
Sbjct: 129 ICTIAPMIFSFWASVKALTPDEKIMSEITATIWSSFGVYLMSLPLILLYYLLKKFL 184

BLAST of Spg025302.1 vs. ExPASy TrEMBL
Match: A0A0A0KFJ5 (PGG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G363010 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-26
Identity = 75/175 (42.86%), Postives = 114/175 (65.14%), Query Frame = 0

Query: 65  KEASVLSIETETGNGKNVSSKDGDRDFLMVITTFIATMAFQAGINPPGGVWDDDDKGHVA 124
           +E + ++ E +T + K +  +  D + LMVI TFI T++FQAG NPPGGVW +   G  A
Sbjct: 9   QETATIAAENQTSSEKTL--QKADIELLMVIITFIGTISFQAGTNPPGGVWQE---GPEA 68

Query: 125 GKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTFMFFSIGSTISF 184
           GKSIMA+K+P+QF++FI GVTV + +SA QL+ L+     N+ S S+ F++ S+G TI+ 
Sbjct: 69  GKSIMASKNPSQFVVFIVGVTVGMVLSAAQLLTLMNELPYNKLSDSRNFIYCSLGITITA 128

Query: 185 MAVAYWNSVTALTPESEMSKVKPMFWFTIGFS-VLLPILVYLSKKLLSHCYKKNC 239
           +  AYW+S+TALTP S + +V+ +    +    V++ I+VY+ KK    C K  C
Sbjct: 129 LGFAYWSSLTALTPSSMILEVRIVLITELSIGVVIVAIIVYIQKK--DVCSKLKC 176

BLAST of Spg025302.1 vs. TAIR 10
Match: AT5G51160.1 (Ankyrin repeat family protein )

HSP 1 Score: 52.0 bits (123), Expect = 7.8e-07
Identity = 37/124 (29.84%), Postives = 58/124 (46.77%), Query Frame = 0

Query: 80  KNVSSKDGDRDFLMVITTFIATMAFQAGINPPGGVWDD----------------DDKGHV 139
           K+  S    R  L+V+ + +AT  FQA + PPGG W D                + + H 
Sbjct: 262 KHRDSPSEARSALLVVASLVATATFQASLTPPGGTWQDSSIPAVSQNTTNVNTTNQQAHT 321

Query: 140 AGKSIMATKSPNQFILFITGVTVCLSVSAMQLVVLLVN-STDNRRSVSKTFMFFSIGSTI 187
           AG+SIM T +   F LF+   T+  SVS   L +L +      +  +    M+FS  +T+
Sbjct: 322 AGQSIMGTFNGVAFTLFVFFNTIGFSVSLSMLNILTLGFPLRFQLQICMIAMYFSHNTTM 381

BLAST of Spg025302.1 vs. TAIR 10
Match: AT5G54710.1 (Ankyrin repeat family protein )

HSP 1 Score: 48.5 bits (114), Expect = 8.6e-06
Identity = 39/151 (25.83%), Postives = 65/151 (43.05%), Query Frame = 0

Query: 89  RDFLMVITTFIATMAFQAGINPPGGVWDDDDKGHVAGKSIMATKSPNQFILFITGVTVCL 148
           R+ + ++   IA++AF  GINPPGGV  D   G   GK+        +       + +  
Sbjct: 424 RNTITIVAVLIASVAFTCGINPPGGVHQD---GPFIGKATAGRTLAFKIFSVANNIALFT 483

Query: 149 SVSAMQLVVLLVNSTDNRRSVSKTFMFFSIGSTISFMAVAYWNSVTALTPESEMSKVKPM 208
           S+S + L+V +++       +        +   ++ MA AY  S     P +E SK    
Sbjct: 484 SLSIVTLLVSIISYRTKALKMCVVIAHKMMWLAVASMATAYAASAWITVPHNEGSKWLVY 543

Query: 209 FWFTIGFSVLLPILVYLSKKLLSHCYKKNCL 240
               I    L  + VY+S  ++ H  KK+ L
Sbjct: 544 TTSAIASVALGSMFVYVSFMMVKHILKKDKL 571

BLAST of Spg025302.1 vs. TAIR 10
Match: AT3G13950.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G13266.1); Has 339 Blast hits to 265 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 339; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-05
Identity = 40/152 (26.32%), Postives = 75/152 (49.34%), Query Frame = 0

Query: 89  RDFLMVITTFIATMAFQAGINPPGGVWDDDD-------------KGHVAGKSIMATKSPN 148
           R  LMV  T IA M+FQ  +NPPGGVW  D+             KG  AG +++  +S  
Sbjct: 34  RGNLMVTATVIAGMSFQVMVNPPGGVWQSDNCSFGNQTGTAPFCKGR-AGTAVLEYESSK 93

Query: 149 Q--FILFITGVTVCLSVSAMQLVVLLVNSTDNRRSVSKTFMFFSIGSTISFMAVAYWNSV 208
           +  +I  I   TV  S S M L++L+++    R  +    +   +   +  ++ A++ ++
Sbjct: 94  RIAYIGMIISSTVSFSTS-MSLILLVISGIRLRNRMIMAILGTFMVVAVLCISAAFFFAI 153

Query: 209 TALTPESEMSKVKPMFWFTIGFSVLLPILVYL 226
             +  + ++  ++ +    +GF V+ P+L+ L
Sbjct: 154 VLVQSDDQI--IRYILLIYVGFWVVFPVLILL 181

BLAST of Spg025302.1 vs. TAIR 10
Match: AT5G50140.1 (Ankyrin repeat family protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-05
Identity = 43/151 (28.48%), Postives = 69/151 (45.70%), Query Frame = 0

Query: 89  RDFLMVITTFIATMAFQAGINPPGGVWDDDDKGHVAGKSIMATKSPNQFILFITGVTVCL 148
           R+ + V+   IA++ F  G+NPPGGV+ D   GH  GK+         F +F    ++ L
Sbjct: 364 RNTITVVAVLIASVTFTCGLNPPGGVYQD---GHFIGKA--TAGGTVAFKVFSVSNSIAL 423

Query: 149 SVSAMQLVVLLVNSTDNRRSVSKTFMFFS---IGSTISFMAVAYWNSVTALTPESEMSKV 208
             S + +V+LL++    R    KTF+  +   I   +  MA AY        P S  +K 
Sbjct: 424 FTS-LCIVILLLSIIPFRTKSLKTFLIITHKMIWLAVIAMASAYVAGTCVTLPHSRGNKW 483

Query: 209 KPMFWFTIGFSVLLPILVYLSKKLLSHCYKK 237
                  I   +L  + ++L  KL +H  +K
Sbjct: 484 VLKATLVIACVMLGGMFIFLWFKLANHMSRK 508

BLAST of Spg025302.1 vs. TAIR 10
Match: AT1G34050.1 (Ankyrin repeat family protein )

HSP 1 Score: 46.2 bits (108), Expect = 4.3e-05
Identity = 38/151 (25.17%), Postives = 71/151 (47.02%), Query Frame = 0

Query: 89  RDFLMVITTFIATMAFQAGINPPGGVWDDDDKGHVAGKSIMATKSPNQFILFITGVTVCL 148
           R+ + ++   IA++AF  GINPPGGV+ +   G   GKS        Q       + +  
Sbjct: 396 RNTITIVAVLIASVAFTCGINPPGGVYQE---GPYKGKSTAGRTLAFQVFSISNNIALFT 455

Query: 149 SVSAMQLVVLLVNSTDNRRSVSKTFMFFS---IGSTISFMAVAYWNSVTALTPESEMSKV 208
           S+    +V+LLV+    R    K F+  +   +   ++ MA+AY ++ + + P  E  + 
Sbjct: 456 SLC---IVILLVSIIPYRTRPLKNFLKLTHRILWVAVASMALAYVSAASIIIPHVEGKRW 515

Query: 209 KPMFWFTIGFSVLLPILVYLSKKLLSHCYKK 237
                 +I   +L  +  +++ K++ H  KK
Sbjct: 516 LFTTVLSISTLMLGGLFAFMTYKVIRHWLKK 540