Spg019832.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg019832.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	N-carbamoylputrescine amidase
Location	scaffold5: 28544549 .. 28547123 (+)
Sequence length	486
RNA-Seq Expression	Spg019832.1
Synteny	Spg019832.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGACTTCTTTTATACCAATTGAAGCCAAAGCAGCCCATCCCGGTTGCCAGAATCCGTTATTTCCTTCTTGGATCAGTAACCTTTTATTTCCTTCTTGGATCAGTAATCCTTCAAGGTCCGTGGGGCTATAGATAGGTAAGGGGATGCTACTAACAAGGCCATTTGGGAAAGGATGAACGCTCCCCCCAATTCCACCACTCGAGGATGAACGCTCTGGCCCGGGAATGTCGGAGTTTTAGGTGTCGGATCCGGATTCCGAATCATGGTCTGAATCTTGAGCCTAGGGCGTTATAGTTGCATTTTTGTCTCCATCTGATCTCTTCCTTAACTAGCGAAAGTGAGCATACTTTAGATGTAATTGACATGTACTCTCGATCAAGAGGTCAGAGGTTCGAATCCCTCACCCTTAAATATCATTGAACTAGAAAAAAAAAACATCCAGTTCTAGGAGTTCTAGGATCTTGAGTTTCAGATCAATCCAGTTCTCACTAAGGGGGTTAGTTTCTTGGTTCAATTACAGAACTTTTAATCGAGAACATTGTATTCTTTTTAAGATATAGAATAACTTGGCCAGCCTTCTGTCTGAAAAGGGGATCCACCAGCTTTCACCATCTTCTTTCAGCATGATTGAGAAAGACCAAAAATTTCAAATGTTGAAGGTAGAAGACTCCCAGAGCAATTGGTTTCAAGTAAATTAGGTAGTGATCAGATGCATTCCTCTCCTTTCTCTCAACTCTGAATTGATAAATCCAATATTCTACTCATTGGAGGTGGAAAATATATCAATAAGTGAGAAGATAGTTAGTTGCCTTGTACTGAATCTAGTGTCGGATCCCTTTCAAAGGGGGATCTGGTTTATTGCTAGATCTTGTTAAAGTTGATTAAATTTTTTCATGCTTTTGGTTATATCTATGATCTTTCCTCTCGGAAAGATAAAAAGAAACAACTATCTAATTCAAGTTGTAAATTTTAAAAATGGAGTTCTTGAATATTAATATTATAGACATCAATTCGTTCCTAAATGCTTTACATGCCCTTTTATATGTGATACAGATTTACGTCTGTATGAACGAAAAAAAAGTGTATTTTTGTTCGCTTTTGATGTCTGCAAAATGCTTCTTTTCATTGAGTTTTTGTTTGGTCTCCAGTTGAAGTTTCCTCCTGTCTGATCCCATGGTGAAGATTTTGTTCACTTTTTAAAAGAAAATTTTCATTTTATTTTATAGATTTAAGTATTACATTCAGAACGTGTAAGATATTTTAGTGCTGAATCCGGTAGAACCTAAATAGTGAGGACAATTTGTTTCATCTCAGTACTTGACTGTACTTCTGATAGAACTTTATGGTCTAATTTGATAATGAGCAAAGTTAGTTGTTGGAGCTAACATGTTTTCGAAAAAAAAAATAAGAAATGCATGTAATAGTTGAAAAAAATGTCAGCAATATTCAGAAGCTAAAGGTGCGAAATCTTTTTTATTTCAGCCATTTGCTGGGATCAGTGGTTTCCAGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCTGAGGTATTGTTTTATCCTACAGCAATTGGGTCTGAACCTCAAGATGATGGACTTGACTCTCGTGATCATTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGGTAAGTTTATAGACGACAATATAGCTGTCTTTTGTGGAAGCCTCGTTGGCCTGCTAATGAACAATCATTGAACTATCAAAAAGTAACTTTGAATAATACTTTTTTGGGTGTGCGTGTAAAGTTTTGCTTTTAAATGGCATTTTTGGTGCTTTTATTGGATATATGAAACTTAGCCTTAAAAAATATCATATTGAATAATACCTGGATGCGCATTATTCCACAGCCACGCATTTTGTATGCTTAAGATACCTCATTAATATAAAAACATTCAGGTGCCATTAGTGGCTTCAAACCGCATTGGAACGGAGATCATTGAGACAGAGCATGGAAAAAGCAAGATCACGTTTTATGGGAATTCTTTTATAGCAGGTACCGTGTAAATGCATGTCAACATTTGCAAAGTTGAAACATCTTGGACTGAAGCTATATGCATAATTGGATTTGTGAGAAGGTACTATTTTAATATGAATGCTTTAATGACAGGACCCACTGGAGAAATTGTTGCTGCTGCTAATGATAAGGATGAAGATGTTCTAGTAGCAGAGTTTGATCTGGATAAAATCAAGTCCAAGAGACATGCTTGGGGAGTATTTCGCGATCGCCGGCCAGAGTTGTACAAGGTGCTTCTGACTTTAGATGGCACCAACCCCGTTTTATAAATTTTGGTCTTGACCCTGTCAGTTTCCTATTCTAGAAAGTTGGAAAATTTAAGCATAAACAGCTTTCATTCCCCTGTTTATGTTTCAAAAGTAAGCATAACGAAGTGTTTATTAAATCATTAATTTAGTTTTGAAAGTGGAAACATATATGACTAGCTTCTGTTATTTCATGTGGAACTTGGATTGCACATCCTTGAGGAAATAAGAAAGGCGCTGAGTTCCCAATTTTATTTGGTTCTAAATCCATTATAGCTGCTTTAAAC

mRNA sequence

ATGAGGACTTCTTTTATACCAATTGAAGCCAAAGCAGCCCATCCCGCCATTTGCTGGGATCAGTGGTTTCCAGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCTGAGGTATTGTTTTATCCTACAGCAATTGGGTCTGAACCTCAAGATGATGGACTTGACTCTCGTGATCATTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGGTGCCATTAGTGGCTTCAAACCGCATTGGAACGGAGATCATTGAGACAGAGCATGGAAAAAGCAAGATCACGTTTTATGGGAATTCTTTTATAGCAGGACCCACTGGAGAAATTGTTGCTGCTGCTAATGATAAGGATGAAGATGTTCTAGTAGCAGAGTTTGATCTGGATAAAATCAAGTCCAAGAGACATGCTTGGGGAGTATTTCGCGATCGCCGGCCAGAGTTGTACAAGGTGCTTCTGACTTTAGATGGCACCAACCCCGTTTTATAA

Coding sequence (CDS)

ATGAGGACTTCTTTTATACCAATTGAAGCCAAAGCAGCCCATCCCGCCATTTGCTGGGATCAGTGGTTTCCAGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCTGAGGTATTGTTTTATCCTACAGCAATTGGGTCTGAACCTCAAGATGATGGACTTGACTCTCGTGATCATTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGGTGCCATTAGTGGCTTCAAACCGCATTGGAACGGAGATCATTGAGACAGAGCATGGAAAAAGCAAGATCACGTTTTATGGGAATTCTTTTATAGCAGGACCCACTGGAGAAATTGTTGCTGCTGCTAATGATAAGGATGAAGATGTTCTAGTAGCAGAGTTTGATCTGGATAAAATCAAGTCCAAGAGACATGCTTGGGGAGTATTTCGCGATCGCCGGCCAGAGTTGTACAAGGTGCTTCTGACTTTAGATGGCACCAACCCCGTTTTATAA

Protein sequence

MRTSFIPIEAKAAHPAICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLVASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRHAWGVFRDRRPELYKVLLTLDGTNPVL

Homology

[Close]

BLAST of Spg019832.1 vs. NCBI nr
Match: KAG7012708.1 (N-carbamoylputrescine amidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 142/146 (97.26%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

[Close]

BLAST of Spg019832.1 vs. NCBI nr
Match: KAG6573627.1 (N-carbamoylputrescine amidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 142/146 (97.26%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

[Close]

BLAST of Spg019832.1 vs. NCBI nr
Match: XP_022945208.1 (N-carbamoylputrescine amidase [Cucurbita moschata])

HSP 1 Score: 302.4 bits (773), Expect = 2.4e-78
Identity = 141/146 (96.58%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGN+FIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNTFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

[Close]

BLAST of Spg019832.1 vs. NCBI nr
Match: XP_023541868.1 (N-carbamoylputrescine amidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 301.6 bits (771), Expect = 4.1e-78
Identity = 141/146 (96.58%), Postives = 145/146 (99.32%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLD IKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDNIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

[Close]

BLAST of Spg019832.1 vs. NCBI nr
Match: XP_022142563.1 (N-carbamoylputrescine amidase [Momordica charantia])

HSP 1 Score: 301.2 bits (770), Expect = 5.3e-78
Identity = 142/146 (97.26%), Postives = 145/146 (99.32%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGA+VVPLV
Sbjct: 155 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGASVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVA+FDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAKFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYK LLTLDGTNPVL
Sbjct: 275 AWGVFRDRRPELYKALLTLDGTNPVL 300

[Close]

BLAST of Spg019832.1 vs. ExPASy Swiss-Prot
Match: Q9XGI9 (N-carbamoylputrescine amidase OS=Solanum lycopersicum OX=4081 GN=CPA PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 2.8e-74
Identity = 130/145 (89.66%), Postives = 138/145 (95.17%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAM LQGAEVLFYPTAIGSEPQDDGLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 155 AICWDQWFPEAARAMALQGAEVLFYPTAIGSEPQDDGLDSRDHWRRVMQGHAGANVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGE+VAAA DK+E VLVA+FDLDKIKSKRH
Sbjct: 215 ASNRIGKEIIETEHGNSEITFYGYSFIAGPTGELVAAAGDKEEAVLVAQFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPV 161
            WGV+RDRRP+LYKVLLTLDG+NPV
Sbjct: 275 GWGVYRDRRPDLYKVLLTLDGSNPV 299

[Close]

BLAST of Spg019832.1 vs. ExPASy Swiss-Prot
Match: Q3HVN1 (N-carbamoylputrescine amidase OS=Solanum tuberosum OX=4113 GN=CPA PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 2.8e-74
Identity = 130/145 (89.66%), Postives = 138/145 (95.17%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAM LQGAEVLFYPTAIGSEPQDDGLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 155 AICWDQWFPEAARAMALQGAEVLFYPTAIGSEPQDDGLDSRDHWRRVMQGHAGANVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGE+VAAA DK+E VLVA+FDLDKIKSKRH
Sbjct: 215 ASNRIGKEIIETEHGNSEITFYGYSFIAGPTGELVAAAGDKEEAVLVAQFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPV 161
            WGV+RDRRP+LYKVLLTLDG+NPV
Sbjct: 275 GWGVYRDRRPDLYKVLLTLDGSNPV 299

[Close]

BLAST of Spg019832.1 vs. ExPASy Swiss-Prot
Match: Q93XI4 (N-carbamoylputrescine amidase OS=Oryza sativa subsp. japonica OX=39947 GN=CPA PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 4.2e-70
Identity = 121/140 (86.43%), Postives = 131/140 (93.57%), Query Frame = 0


Query: 17  ICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLVA 76
           ICWDQWFPE ARAMVLQGAE+LFYPTAIGSEPQD+ LDSR+HWKRVMQGHAGAN+VPLVA
Sbjct: 159 ICWDQWFPECARAMVLQGAEILFYPTAIGSEPQDNNLDSREHWKRVMQGHAGANLVPLVA 218

Query: 77  SNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRHA 136
           SNRIG E +ETEHG+S ITF+GNSFIAGPTGEIV  ANDKDEDVLVAEFDLD+IKS RH 
Sbjct: 219 SNRIGRETVETEHGESTITFFGNSFIAGPTGEIVKLANDKDEDVLVAEFDLDEIKSTRHG 278

Query: 137 WGVFRDRRPELYKVLLTLDG 157
           WG+FRDRRP+LYKVLLTLDG
Sbjct: 279 WGIFRDRRPDLYKVLLTLDG 298

[Close]

BLAST of Spg019832.1 vs. ExPASy Swiss-Prot
Match: Q8VYF5 (N-carbamoylputrescine amidase OS=Arabidopsis thaliana OX=3702 GN=CPA PE=1 SV=2)

HSP 1 Score: 265.0 bits (676), Expect = 5.5e-70
Identity = 124/141 (87.94%), Postives = 132/141 (93.62%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 157 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 216

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGEIVA A+DK E VLVA+FDLD IKSKR 
Sbjct: 217 ASNRIGKEIIETEHGPSQITFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQ 276

Query: 136 AWGVFRDRRPELYKVLLTLDG 157
           +WGVFRDRRP+LYKVLLT+DG
Sbjct: 277 SWGVFRDRRPDLYKVLLTMDG 297

[Close]

BLAST of Spg019832.1 vs. ExPASy Swiss-Prot
Match: O59829 (Probable nitrilase C965.09 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC965.09 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 5.8e-19
Identity = 53/138 (38.41%), Postives = 78/138 (56.52%), Query Frame = 0


Query: 17  ICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLVA 76
           ICWD  FPE AR   L GA++L   T   +   DD       W  V +  A  N +PLVA
Sbjct: 149 ICWDTAFPEVARIHALNGADLLVVATNWENPYSDD-------WDLVTKARAFENCIPLVA 208

Query: 77  SNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKS-KRH 136
           +NR+GT+         K++F+G+S I GPTG+++ A +++ E V+    DLD  K  +++
Sbjct: 209 ANRVGTD--------EKLSFFGHSKIIGPTGKVIKALDEEKEGVISYTVDLDDAKPLRKN 268

Query: 137 AWGVFRDRRPELYKVLLT 154
            +  F DR P+LYK LL+
Sbjct: 269 YYTFFEDRMPDLYKRLLS 271

[Close]

BLAST of Spg019832.1 vs. ExPASy TrEMBL
Match: A0A6J1G065 (N-carbamoylputrescine amidase OS=Cucurbita moschata OX=3662 GN=LOC111449518 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.2e-78
Identity = 141/146 (96.58%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGN+FIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNTFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

[Close]

BLAST of Spg019832.1 vs. ExPASy TrEMBL
Match: A0A6J1CNJ5 (N-carbamoylputrescine amidase OS=Momordica charantia OX=3673 GN=LOC111012646 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.6e-78
Identity = 142/146 (97.26%), Postives = 145/146 (99.32%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGA+VVPLV
Sbjct: 155 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGASVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVA+FDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAKFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYK LLTLDGTNPVL
Sbjct: 275 AWGVFRDRRPELYKALLTLDGTNPVL 300

[Close]

BLAST of Spg019832.1 vs. ExPASy TrEMBL
Match: A0A6J1HPT8 (N-carbamoylputrescine amidase OS=Cucurbita maxima OX=3661 GN=LOC111466602 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.4e-78
Identity = 140/146 (95.89%), Postives = 145/146 (99.32%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           A+CWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AVCWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLT DGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTSDGTNPIL 300

[Close]

BLAST of Spg019832.1 vs. ExPASy TrEMBL
Match: A0A0A0LT48 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G166770 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 4.1e-76
Identity = 139/146 (95.21%), Postives = 142/146 (97.26%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDS +HWKRVMQGHAGANVVPLV
Sbjct: 155 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSCNHWKRVMQGHAGANVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKS ITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSTITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDG NP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGINPIL 300

[Close]

BLAST of Spg019832.1 vs. ExPASy TrEMBL
Match: A0A5D3E4I8 (N-carbamoylputrescine amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G002620 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 5.4e-76
Identity = 139/146 (95.21%), Postives = 141/146 (96.58%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDS DHWKRVMQGHAGANVVPLV
Sbjct: 155 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSCDHWKRVMQGHAGANVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHGKS ITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGREIIETEHGKSTITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDG NP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGINPIL 300

[Close]

BLAST of Spg019832.1 vs. TAIR 10
Match: AT2G27450.1 (nitrilase-like protein 1 )

HSP 1 Score: 265.0 bits (676), Expect = 3.9e-71
Identity = 124/141 (87.94%), Postives = 132/141 (93.62%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 157 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 216

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGEIVA A+DK E VLVA+FDLD IKSKR 
Sbjct: 217 ASNRIGKEIIETEHGPSQITFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQ 276

Query: 136 AWGVFRDRRPELYKVLLTLDG 157
           +WGVFRDRRP+LYKVLLT+DG
Sbjct: 277 SWGVFRDRRPDLYKVLLTMDG 297

[Close]

BLAST of Spg019832.1 vs. TAIR 10
Match: AT2G27450.2 (nitrilase-like protein 1 )

HSP 1 Score: 265.0 bits (676), Expect = 3.9e-71
Identity = 124/141 (87.94%), Postives = 132/141 (93.62%), Query Frame = 0


Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 184 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 243

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGEIVA A+DK E VLVA+FDLD IKSKR 
Sbjct: 244 ASNRIGKEIIETEHGPSQITFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQ 303

Query: 136 AWGVFRDRRPELYKVLLTLDG 157
           +WGVFRDRRP+LYKVLLT+DG
Sbjct: 304 SWGVFRDRRPDLYKVLLTMDG 324

The following BLAST results are available for this feature:

BLAST of Spg019832.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012708.1	1.1e-78	97.26	N-carbamoylputrescine amidase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6573627.1	1.1e-78	97.26	N-carbamoylputrescine amidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022945208.1	2.4e-78	96.58	N-carbamoylputrescine amidase [Cucurbita moschata]	[more]
XP_023541868.1	4.1e-78	96.58	N-carbamoylputrescine amidase [Cucurbita pepo subsp. pepo]	[more]
XP_022142563.1	5.3e-78	97.26	N-carbamoylputrescine amidase [Momordica charantia]	[more]

BLAST of Spg019832.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI9	2.8e-74	89.66	N-carbamoylputrescine amidase OS=Solanum lycopersicum OX=4081 GN=CPA PE=2 SV=1	[more]
Q3HVN1	2.8e-74	89.66	N-carbamoylputrescine amidase OS=Solanum tuberosum OX=4113 GN=CPA PE=2 SV=1	[more]
Q93XI4	4.2e-70	86.43	N-carbamoylputrescine amidase OS=Oryza sativa subsp. japonica OX=39947 GN=CPA PE...	[more]
Q8VYF5	5.5e-70	87.94	N-carbamoylputrescine amidase OS=Arabidopsis thaliana OX=3702 GN=CPA PE=1 SV=2	[more]
O59829	5.8e-19	38.41	Probable nitrilase C965.09 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843...	[more]

BLAST of Spg019832.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G065	1.2e-78	96.58	N-carbamoylputrescine amidase OS=Cucurbita moschata OX=3662 GN=LOC111449518 PE=4...	[more]
A0A6J1CNJ5	2.6e-78	97.26	N-carbamoylputrescine amidase OS=Momordica charantia OX=3673 GN=LOC111012646 PE=...	[more]
A0A6J1HPT8	3.4e-78	95.89	N-carbamoylputrescine amidase OS=Cucurbita maxima OX=3661 GN=LOC111466602 PE=4 S...	[more]
A0A0A0LT48	4.1e-76	95.21	CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G16677...	[more]
A0A5D3E4I8	5.4e-76	95.21	N-carbamoylputrescine amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Spg019832.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27450.1	3.9e-71	87.94	nitrilase-like protein 1	[more]
AT2G27450.2	3.9e-71	87.94	nitrilase-like protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 15..133 e-value: 7.7E-23 score: 81.1
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 1..127 score: 27.956469
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	3.60.110.10		coord: 5..161 e-value: 3.4E-48 score: 166.0
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	56317	Carbon-nitrogen hydrolase	coord: 15..151
None	No IPR available	PANTHER	PTHR43674	NITRILASE C965.09-RELATED	coord: 16..153
None	No IPR available	PANTHER	PTHR43674:SF6	NITRILASE C965.09-RELATED	coord: 16..153

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg019832	Spg019832	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg019832.1-cds	Spg019832.1-cds-scaffold5:28544549..28544594	CDS
Spg019832.1-cds	Spg019832.1-cds-scaffold5:28546035..28546201	CDS
Spg019832.1-cds	Spg019832.1-cds-scaffold5:28546474..28546570	CDS
Spg019832.1-cds	Spg019832.1-cds-scaffold5:28546685..28546860	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg019832.1-three_prime_utr	Spg019832.1-three_prime_utr-scaffold5:28546861..28547123	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg019832.1	Spg019832.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0033388	putrescine biosynthetic process from arginine
molecular_function	GO:0050126	N-carbamoylputrescine amidase activity