Spg019832 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg019832
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionN-carbamoylputrescine amidase
Locationscaffold5: 28544549 .. 28547123 (+)
RNA-Seq ExpressionSpg019832
SyntenySpg019832
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGACTTCTTTTATACCAATTGAAGCCAAAGCAGCCCATCCCGGTTGCCAGAATCCGTTATTTCCTTCTTGGATCAGTAACCTTTTATTTCCTTCTTGGATCAGTAATCCTTCAAGGTCCGTGGGGCTATAGATAGGTAAGGGGATGCTACTAACAAGGCCATTTGGGAAAGGATGAACGCTCCCCCCAATTCCACCACTCGAGGATGAACGCTCTGGCCCGGGAATGTCGGAGTTTTAGGTGTCGGATCCGGATTCCGAATCATGGTCTGAATCTTGAGCCTAGGGCGTTATAGTTGCATTTTTGTCTCCATCTGATCTCTTCCTTAACTAGCGAAAGTGAGCATACTTTAGATGTAATTGACATGTACTCTCGATCAAGAGGTCAGAGGTTCGAATCCCTCACCCTTAAATATCATTGAACTAGAAAAAAAAAACATCCAGTTCTAGGAGTTCTAGGATCTTGAGTTTCAGATCAATCCAGTTCTCACTAAGGGGGTTAGTTTCTTGGTTCAATTACAGAACTTTTAATCGAGAACATTGTATTCTTTTTAAGATATAGAATAACTTGGCCAGCCTTCTGTCTGAAAAGGGGATCCACCAGCTTTCACCATCTTCTTTCAGCATGATTGAGAAAGACCAAAAATTTCAAATGTTGAAGGTAGAAGACTCCCAGAGCAATTGGTTTCAAGTAAATTAGGTAGTGATCAGATGCATTCCTCTCCTTTCTCTCAACTCTGAATTGATAAATCCAATATTCTACTCATTGGAGGTGGAAAATATATCAATAAGTGAGAAGATAGTTAGTTGCCTTGTACTGAATCTAGTGTCGGATCCCTTTCAAAGGGGGATCTGGTTTATTGCTAGATCTTGTTAAAGTTGATTAAATTTTTTCATGCTTTTGGTTATATCTATGATCTTTCCTCTCGGAAAGATAAAAAGAAACAACTATCTAATTCAAGTTGTAAATTTTAAAAATGGAGTTCTTGAATATTAATATTATAGACATCAATTCGTTCCTAAATGCTTTACATGCCCTTTTATATGTGATACAGATTTACGTCTGTATGAACGAAAAAAAAGTGTATTTTTGTTCGCTTTTGATGTCTGCAAAATGCTTCTTTTCATTGAGTTTTTGTTTGGTCTCCAGTTGAAGTTTCCTCCTGTCTGATCCCATGGTGAAGATTTTGTTCACTTTTTAAAAGAAAATTTTCATTTTATTTTATAGATTTAAGTATTACATTCAGAACGTGTAAGATATTTTAGTGCTGAATCCGGTAGAACCTAAATAGTGAGGACAATTTGTTTCATCTCAGTACTTGACTGTACTTCTGATAGAACTTTATGGTCTAATTTGATAATGAGCAAAGTTAGTTGTTGGAGCTAACATGTTTTCGAAAAAAAAAATAAGAAATGCATGTAATAGTTGAAAAAAATGTCAGCAATATTCAGAAGCTAAAGGTGCGAAATCTTTTTTATTTCAGCCATTTGCTGGGATCAGTGGTTTCCAGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCTGAGGTATTGTTTTATCCTACAGCAATTGGGTCTGAACCTCAAGATGATGGACTTGACTCTCGTGATCATTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGGTAAGTTTATAGACGACAATATAGCTGTCTTTTGTGGAAGCCTCGTTGGCCTGCTAATGAACAATCATTGAACTATCAAAAAGTAACTTTGAATAATACTTTTTTGGGTGTGCGTGTAAAGTTTTGCTTTTAAATGGCATTTTTGGTGCTTTTATTGGATATATGAAACTTAGCCTTAAAAAATATCATATTGAATAATACCTGGATGCGCATTATTCCACAGCCACGCATTTTGTATGCTTAAGATACCTCATTAATATAAAAACATTCAGGTGCCATTAGTGGCTTCAAACCGCATTGGAACGGAGATCATTGAGACAGAGCATGGAAAAAGCAAGATCACGTTTTATGGGAATTCTTTTATAGCAGGTACCGTGTAAATGCATGTCAACATTTGCAAAGTTGAAACATCTTGGACTGAAGCTATATGCATAATTGGATTTGTGAGAAGGTACTATTTTAATATGAATGCTTTAATGACAGGACCCACTGGAGAAATTGTTGCTGCTGCTAATGATAAGGATGAAGATGTTCTAGTAGCAGAGTTTGATCTGGATAAAATCAAGTCCAAGAGACATGCTTGGGGAGTATTTCGCGATCGCCGGCCAGAGTTGTACAAGGTGCTTCTGACTTTAGATGGCACCAACCCCGTTTTATAAATTTTGGTCTTGACCCTGTCAGTTTCCTATTCTAGAAAGTTGGAAAATTTAAGCATAAACAGCTTTCATTCCCCTGTTTATGTTTCAAAAGTAAGCATAACGAAGTGTTTATTAAATCATTAATTTAGTTTTGAAAGTGGAAACATATATGACTAGCTTCTGTTATTTCATGTGGAACTTGGATTGCACATCCTTGAGGAAATAAGAAAGGCGCTGAGTTCCCAATTTTATTTGGTTCTAAATCCATTATAGCTGCTTTAAAC

mRNA sequence

ATGAGGACTTCTTTTATACCAATTGAAGCCAAAGCAGCCCATCCCGCCATTTGCTGGGATCAGTGGTTTCCAGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCTGAGGTATTGTTTTATCCTACAGCAATTGGGTCTGAACCTCAAGATGATGGACTTGACTCTCGTGATCATTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGGTGCCATTAGTGGCTTCAAACCGCATTGGAACGGAGATCATTGAGACAGAGCATGGAAAAAGCAAGATCACGTTTTATGGGAATTCTTTTATAGCAGGACCCACTGGAGAAATTGTTGCTGCTGCTAATGATAAGGATGAAGATGTTCTAGTAGCAGAGTTTGATCTGGATAAAATCAAGTCCAAGAGACATGCTTGGGGAGTATTTCGCGATCGCCGGCCAGAGTTGTACAAGGTGCTTCTGACTTTAGATGGCACCAACCCCGTTTTATAA

Coding sequence (CDS)

ATGAGGACTTCTTTTATACCAATTGAAGCCAAAGCAGCCCATCCCGCCATTTGCTGGGATCAGTGGTTTCCAGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCTGAGGTATTGTTTTATCCTACAGCAATTGGGTCTGAACCTCAAGATGATGGACTTGACTCTCGTGATCATTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGGTGCCATTAGTGGCTTCAAACCGCATTGGAACGGAGATCATTGAGACAGAGCATGGAAAAAGCAAGATCACGTTTTATGGGAATTCTTTTATAGCAGGACCCACTGGAGAAATTGTTGCTGCTGCTAATGATAAGGATGAAGATGTTCTAGTAGCAGAGTTTGATCTGGATAAAATCAAGTCCAAGAGACATGCTTGGGGAGTATTTCGCGATCGCCGGCCAGAGTTGTACAAGGTGCTTCTGACTTTAGATGGCACCAACCCCGTTTTATAA

Protein sequence

MRTSFIPIEAKAAHPAICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLVASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRHAWGVFRDRRPELYKVLLTLDGTNPVL
Homology
BLAST of Spg019832 vs. NCBI nr
Match: KAG7012708.1 (N-carbamoylputrescine amidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 142/146 (97.26%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

BLAST of Spg019832 vs. NCBI nr
Match: KAG6573627.1 (N-carbamoylputrescine amidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 142/146 (97.26%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

BLAST of Spg019832 vs. NCBI nr
Match: XP_022945208.1 (N-carbamoylputrescine amidase [Cucurbita moschata])

HSP 1 Score: 302.4 bits (773), Expect = 2.4e-78
Identity = 141/146 (96.58%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGN+FIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNTFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

BLAST of Spg019832 vs. NCBI nr
Match: XP_023541868.1 (N-carbamoylputrescine amidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 301.6 bits (771), Expect = 4.1e-78
Identity = 141/146 (96.58%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLD IKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDNIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

BLAST of Spg019832 vs. NCBI nr
Match: XP_022142563.1 (N-carbamoylputrescine amidase [Momordica charantia])

HSP 1 Score: 301.2 bits (770), Expect = 5.3e-78
Identity = 142/146 (97.26%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGA+VVPLV
Sbjct: 155 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGASVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVA+FDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAKFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYK LLTLDGTNPVL
Sbjct: 275 AWGVFRDRRPELYKALLTLDGTNPVL 300

BLAST of Spg019832 vs. ExPASy Swiss-Prot
Match: Q9XGI9 (N-carbamoylputrescine amidase OS=Solanum lycopersicum OX=4081 GN=CPA PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 2.8e-74
Identity = 130/145 (89.66%), Postives = 138/145 (95.17%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAM LQGAEVLFYPTAIGSEPQDDGLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 155 AICWDQWFPEAARAMALQGAEVLFYPTAIGSEPQDDGLDSRDHWRRVMQGHAGANVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGE+VAAA DK+E VLVA+FDLDKIKSKRH
Sbjct: 215 ASNRIGKEIIETEHGNSEITFYGYSFIAGPTGELVAAAGDKEEAVLVAQFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPV 161
            WGV+RDRRP+LYKVLLTLDG+NPV
Sbjct: 275 GWGVYRDRRPDLYKVLLTLDGSNPV 299

BLAST of Spg019832 vs. ExPASy Swiss-Prot
Match: Q3HVN1 (N-carbamoylputrescine amidase OS=Solanum tuberosum OX=4113 GN=CPA PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 2.8e-74
Identity = 130/145 (89.66%), Postives = 138/145 (95.17%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAM LQGAEVLFYPTAIGSEPQDDGLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 155 AICWDQWFPEAARAMALQGAEVLFYPTAIGSEPQDDGLDSRDHWRRVMQGHAGANVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGE+VAAA DK+E VLVA+FDLDKIKSKRH
Sbjct: 215 ASNRIGKEIIETEHGNSEITFYGYSFIAGPTGELVAAAGDKEEAVLVAQFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPV 161
            WGV+RDRRP+LYKVLLTLDG+NPV
Sbjct: 275 GWGVYRDRRPDLYKVLLTLDGSNPV 299

BLAST of Spg019832 vs. ExPASy Swiss-Prot
Match: Q93XI4 (N-carbamoylputrescine amidase OS=Oryza sativa subsp. japonica OX=39947 GN=CPA PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 4.2e-70
Identity = 121/140 (86.43%), Postives = 131/140 (93.57%), Query Frame = 0

Query: 17  ICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLVA 76
           ICWDQWFPE ARAMVLQGAE+LFYPTAIGSEPQD+ LDSR+HWKRVMQGHAGAN+VPLVA
Sbjct: 159 ICWDQWFPECARAMVLQGAEILFYPTAIGSEPQDNNLDSREHWKRVMQGHAGANLVPLVA 218

Query: 77  SNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRHA 136
           SNRIG E +ETEHG+S ITF+GNSFIAGPTGEIV  ANDKDEDVLVAEFDLD+IKS RH 
Sbjct: 219 SNRIGRETVETEHGESTITFFGNSFIAGPTGEIVKLANDKDEDVLVAEFDLDEIKSTRHG 278

Query: 137 WGVFRDRRPELYKVLLTLDG 157
           WG+FRDRRP+LYKVLLTLDG
Sbjct: 279 WGIFRDRRPDLYKVLLTLDG 298

BLAST of Spg019832 vs. ExPASy Swiss-Prot
Match: Q8VYF5 (N-carbamoylputrescine amidase OS=Arabidopsis thaliana OX=3702 GN=CPA PE=1 SV=2)

HSP 1 Score: 265.0 bits (676), Expect = 5.5e-70
Identity = 124/141 (87.94%), Postives = 132/141 (93.62%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 157 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 216

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGEIVA A+DK E VLVA+FDLD IKSKR 
Sbjct: 217 ASNRIGKEIIETEHGPSQITFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQ 276

Query: 136 AWGVFRDRRPELYKVLLTLDG 157
           +WGVFRDRRP+LYKVLLT+DG
Sbjct: 277 SWGVFRDRRPDLYKVLLTMDG 297

BLAST of Spg019832 vs. ExPASy Swiss-Prot
Match: O59829 (Probable nitrilase C965.09 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC965.09 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 5.8e-19
Identity = 53/138 (38.41%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 17  ICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLVA 76
           ICWD  FPE AR   L GA++L   T   +   DD       W  V +  A  N +PLVA
Sbjct: 149 ICWDTAFPEVARIHALNGADLLVVATNWENPYSDD-------WDLVTKARAFENCIPLVA 208

Query: 77  SNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKS-KRH 136
           +NR+GT+         K++F+G+S I GPTG+++ A +++ E V+    DLD  K  +++
Sbjct: 209 ANRVGTD--------EKLSFFGHSKIIGPTGKVIKALDEEKEGVISYTVDLDDAKPLRKN 268

Query: 137 AWGVFRDRRPELYKVLLT 154
            +  F DR P+LYK LL+
Sbjct: 269 YYTFFEDRMPDLYKRLLS 271

BLAST of Spg019832 vs. ExPASy TrEMBL
Match: A0A6J1G065 (N-carbamoylputrescine amidase OS=Cucurbita moschata OX=3662 GN=LOC111449518 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.2e-78
Identity = 141/146 (96.58%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AICWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGN+FIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNTFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGTNPIL 300

BLAST of Spg019832 vs. ExPASy TrEMBL
Match: A0A6J1CNJ5 (N-carbamoylputrescine amidase OS=Momordica charantia OX=3673 GN=LOC111012646 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.6e-78
Identity = 142/146 (97.26%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGA+VVPLV
Sbjct: 155 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGASVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVA+FDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAKFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYK LLTLDGTNPVL
Sbjct: 275 AWGVFRDRRPELYKALLTLDGTNPVL 300

BLAST of Spg019832 vs. ExPASy TrEMBL
Match: A0A6J1HPT8 (N-carbamoylputrescine amidase OS=Cucurbita maxima OX=3661 GN=LOC111466602 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.4e-78
Identity = 140/146 (95.89%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           A+CWDQWFPEAARA+VLQGAE+LFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGAN+VPLV
Sbjct: 155 AVCWDQWFPEAARALVLQGAEILFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANLVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLT DGTNP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTSDGTNPIL 300

BLAST of Spg019832 vs. ExPASy TrEMBL
Match: A0A0A0LT48 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G166770 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 4.1e-76
Identity = 139/146 (95.21%), Postives = 142/146 (97.26%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDS +HWKRVMQGHAGANVVPLV
Sbjct: 155 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSCNHWKRVMQGHAGANVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIGTEIIETEHGKS ITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGTEIIETEHGKSTITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDG NP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGINPIL 300

BLAST of Spg019832 vs. ExPASy TrEMBL
Match: A0A5D3E4I8 (N-carbamoylputrescine amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G002620 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 5.4e-76
Identity = 139/146 (95.21%), Postives = 141/146 (96.58%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDS DHWKRVMQGHAGANVVPLV
Sbjct: 155 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSCDHWKRVMQGHAGANVVPLV 214

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHGKS ITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH
Sbjct: 215 ASNRIGREIIETEHGKSTITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 274

Query: 136 AWGVFRDRRPELYKVLLTLDGTNPVL 162
           AWGVFRDRRPELYKVLLTLDG NP+L
Sbjct: 275 AWGVFRDRRPELYKVLLTLDGINPIL 300

BLAST of Spg019832 vs. TAIR 10
Match: AT2G27450.1 (nitrilase-like protein 1 )

HSP 1 Score: 265.0 bits (676), Expect = 3.9e-71
Identity = 124/141 (87.94%), Postives = 132/141 (93.62%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 157 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 216

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGEIVA A+DK E VLVA+FDLD IKSKR 
Sbjct: 217 ASNRIGKEIIETEHGPSQITFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQ 276

Query: 136 AWGVFRDRRPELYKVLLTLDG 157
           +WGVFRDRRP+LYKVLLT+DG
Sbjct: 277 SWGVFRDRRPDLYKVLLTMDG 297

BLAST of Spg019832 vs. TAIR 10
Match: AT2G27450.2 (nitrilase-like protein 1 )

HSP 1 Score: 265.0 bits (676), Expect = 3.9e-71
Identity = 124/141 (87.94%), Postives = 132/141 (93.62%), Query Frame = 0

Query: 16  AICWDQWFPEAARAMVLQGAEVLFYPTAIGSEPQDDGLDSRDHWKRVMQGHAGANVVPLV 75
           AICWDQWFPEAARAMVLQGAE+LFYPTAIGSEPQD GLDSRDHW+RVMQGHAGANVVPLV
Sbjct: 184 AICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 243

Query: 76  ASNRIGTEIIETEHGKSKITFYGNSFIAGPTGEIVAAANDKDEDVLVAEFDLDKIKSKRH 135
           ASNRIG EIIETEHG S+ITFYG SFIAGPTGEIVA A+DK E VLVA+FDLD IKSKR 
Sbjct: 244 ASNRIGKEIIETEHGPSQITFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQ 303

Query: 136 AWGVFRDRRPELYKVLLTLDG 157
           +WGVFRDRRP+LYKVLLT+DG
Sbjct: 304 SWGVFRDRRPDLYKVLLTMDG 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012708.11.1e-7897.26N-carbamoylputrescine amidase [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6573627.11.1e-7897.26N-carbamoylputrescine amidase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022945208.12.4e-7896.58N-carbamoylputrescine amidase [Cucurbita moschata][more]
XP_023541868.14.1e-7896.58N-carbamoylputrescine amidase [Cucurbita pepo subsp. pepo][more]
XP_022142563.15.3e-7897.26N-carbamoylputrescine amidase [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9XGI92.8e-7489.66N-carbamoylputrescine amidase OS=Solanum lycopersicum OX=4081 GN=CPA PE=2 SV=1[more]
Q3HVN12.8e-7489.66N-carbamoylputrescine amidase OS=Solanum tuberosum OX=4113 GN=CPA PE=2 SV=1[more]
Q93XI44.2e-7086.43N-carbamoylputrescine amidase OS=Oryza sativa subsp. japonica OX=39947 GN=CPA PE... [more]
Q8VYF55.5e-7087.94N-carbamoylputrescine amidase OS=Arabidopsis thaliana OX=3702 GN=CPA PE=1 SV=2[more]
O598295.8e-1938.41Probable nitrilase C965.09 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843... [more]
Match NameE-valueIdentityDescription
A0A6J1G0651.2e-7896.58N-carbamoylputrescine amidase OS=Cucurbita moschata OX=3662 GN=LOC111449518 PE=4... [more]
A0A6J1CNJ52.6e-7897.26N-carbamoylputrescine amidase OS=Momordica charantia OX=3673 GN=LOC111012646 PE=... [more]
A0A6J1HPT83.4e-7895.89N-carbamoylputrescine amidase OS=Cucurbita maxima OX=3661 GN=LOC111466602 PE=4 S... [more]
A0A0A0LT484.1e-7695.21CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G16677... [more]
A0A5D3E4I85.4e-7695.21N-carbamoylputrescine amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
AT2G27450.13.9e-7187.94nitrilase-like protein 1 [more]
AT2G27450.23.9e-7187.94nitrilase-like protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003010Carbon-nitrogen hydrolasePFAMPF00795CN_hydrolasecoord: 15..133
e-value: 7.7E-23
score: 81.1
IPR003010Carbon-nitrogen hydrolasePROSITEPS50263CN_HYDROLASEcoord: 1..127
score: 27.956469
IPR036526Carbon-nitrogen hydrolase superfamilyGENE3D3.60.110.10coord: 5..161
e-value: 3.4E-48
score: 166.0
IPR036526Carbon-nitrogen hydrolase superfamilySUPERFAMILY56317Carbon-nitrogen hydrolasecoord: 15..151
NoneNo IPR availablePANTHERPTHR43674NITRILASE C965.09-RELATEDcoord: 16..153
NoneNo IPR availablePANTHERPTHR43674:SF6NITRILASE C965.09-RELATEDcoord: 16..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg019832.1Spg019832.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033388 putrescine biosynthetic process from arginine
biological_process GO:0006807 nitrogen compound metabolic process
molecular_function GO:0050126 N-carbamoylputrescine amidase activity