Sgr004975.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr004975.1
Type	mRNA
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	transmembrane emp24 domain-containing protein p24beta2
Location	tig00003470: 44878 .. 47885 (-)
Sequence length	633
RNA-Seq Expression	Sgr004975.1
Synteny	Sgr004975.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCGATGGGCTATGAGACGTGTGGTGATGATGACGTTTGCGGGGGTATTGCTATTGAGCCTGAGAAGGTCAGAAGGGATTAGGTTTGTGATAGACAGGGAAGAATGCTTCTCTCACAATGTTCAGTACGAGGGTGATACTGTTCATGTTTCTTTCGTCGTAATCAAGGCAGATTCGCCCTGGCACTACGGCGACGACGGTGTCGATCTTGTGGTATCATTCTTCCCCCATCCCCCCCCCCCCCCCCCATTTTCCTTGCCTTTGTCTTGCTTTCCGTGAACCCCCCCTCCCCAAACCAAAAAGAAAGAAAGAAAAAAACCGGGCGAGTAAGATTCTGGCCTTTGTGTTTCACTTATTACCTGTTAATTCTTAGATATGGTGTTCGTAATTCTTGTTTCAAGAAAATGGGAAATTGGTTTCTTAATTCTTCTTGGGGTGCTTGAGGAATAATACTAAAACTAGAATCGGCAGAGACTTATCCTAGTATAAGAGGTGGTTAGTTTGTAAGTTGTTTTTTGGAGATGCTTTGATTTGTCTAGAGAGAGAGAGACCTCCTAATGATAAAAGTTACGTCTTAGTTCTTGGGGTGCTAGAGGACTATTACTAAAACTAGATTGGGCAAACACTTATCTTAGTATAAAGTTATTTTTGTGGAAATTCTTTGATGATTTTTGTGTGTGTGTGTGTGTGTGTGGGAAGGGGGGGGGGGGGGTGGAAGAGAGAGATGGAGGGAGGGATGGAGAGAGAGAGAGGAAGATGCTTTGATTTGGAGAGAGAGATACATCCAAATGATAGAAGTTACCCCTTTAGTGTCTATGCTAAATAAACCATCCTATAGTATAGGGTCTGTAGAAACTGCTTTTGAAGTTAGTTCTTTACCAAATAAGTTATACTCAAGAGTATGTAGAATTCTTGGCCACTTTATTGCTGAGAATGGATGCAAGAGCTTCGTGTTACTTCAAATTGATGAATCAACTTTATATCTCTGAAGGTGCTCATTTAATCTTATTTCTTATTCTGTTATAACTTGGAGGTGTTGGGCAGCTCTTCGCAATATGGATTGAACGTCAGATAGTATTTCTGATACATTTTTAGAGACTGTTGTGTGAGACTTAATTGACAAGTGCCTTACTGGAAGAAAAGTTCAGTTATTAGAGAGACATAAAGAACTGGAAAAACTTTTGAGAGACTATAGAATCTCTATTGGAATGGGTTGTTATACATAGGTCCTGTTTCATCCTTAGAATCTTTATTGTATTCATTCCTGTACTACACGGTCAAGGGGAAAGAATCTTTATTGTATTCATTCCTGTACTACACGGTCAAGAAGCCTTCCCCTCTGTGTTCAAATTGAGTCGGTTATTTTCTAAATGAATTAGCAGTTATGATGCTGTATAGAGACTATATCGGTGTTTAAATCCAGTAAAACCTATGAAAGATTGCAGCACTTAAAGAATATAATGATCAACTTGTAAAGTGTTTTTTCTTTCTTTCTTTCGGTTTTGCTCTTCTTATGGAGTTCTTATATGGGATTTGATTTGAGATCCTTCTGTTATATAGCTTTTGCCTGTAGAATTTGCTGTTAATCCCATATTTTTGTTTGAAATCTAGATAAAGGGCCCTTCTGGTGAACATCTTCATGATTTTCGTGACAAGACTAGTGAAAAGCATGAATTTATTGCTCACAACAAGGGGCTTCATCGATTCTGCTTCACGAACAAGTCTCCGTATCATGAAACCATAGACTTTGATGTGCACGTCGGTCACTTTTCGTATCATGAGCAGCATGCAAAAGATGGTGAGATGTCATTGCCTGTTTTGTATGTTCTAACCTTCCACAAGTTAGAATCAATCTCTAAAACTGGTGCACATGCCTGCAGAGCATTTCAACCCTTTGCTGGATCAAATATCTAAGTTGGAGGAAGCTCTTTACAATATTCAGTTTGAACAGCATTGGTTGGAGGCTCAGACTGACCGCCAGGCAATAGGTAATGTTGACGAATGTATCTCAGACTTAAATAATTTCCCCAATATCAGCCAAGGAATTTAGTTTCTTTGTTGATGTGTTTGCTTCTTAACATCAAGTGACTTCCAAATCTAAACACATCAATTAAATAATCTCTCTAAAACAGATTTTAGGCATTATTTATGCACCAATGATCATGATAGTAGCTTTCTTTTTCATCCTTGTTGCTGAGACTCCGTTAGAATAACGGACATAATGGCATTTTCTGGATACTTGACTGCATACATGTGTAAGAGAATGATTTGGGTGTTGTTCGGGACCTTCAAATGTTCTTCAAGAACAAACAGAGGTTGAACAATAGAATACTGATATGCCACAACATATGAATGATTTTCCCAATATATCTTTATAGAACAATTCTAAAAATGTGTCACAAAAAAGCATACATTAGAACACTCCTTACGTAGTCCACAGTTACTTTTTGATGCAGAAGTGGAATTATATAAATTCTTCGTACGAAGATTACGAGTAACATGCATTAGAACTTTGATCAAATAGAAGCCATGCTTAATTTGGAGGTGTAGTTCATGGGTTGAGATGGGTTAAGAGACAGCCATTGTTCGGATTTCATATTTATCCATCTCTAACTACTGAGTAAGCCGATAGATCCAACCCAACCTGTACATGTTGAGTAGAGTTGATTGGGTTTTTTTTAACACTCTTAGTCTTAGCTTAGTTCCCATGGCAGATGGGTCTTAGAGGTATCCAGCCAGAAACCTACTTAGCTTAATCAAGAACTTGGTCACATTTCTGGTTCAATCTCGATTCTGGGTTGCCAATTGAAACAACGAAAACCGTCATGGATTTGTTGCTGATGTTTTTGTGGGGGGTGTGTTTGATTGCAGTGAATGATGGAATGAGCAGGAGGGCAATCCACAAGGCAATGTTTGAATCAGCAGCACTGGTAGGGGCCAGTGTCCTACAAGTCTACTTGCTGCAGCGCTTGTTTGAAAGGAAGCTTGGGACATCCAGAGTTTAA

mRNA sequence

ATGGAGCGATGGGCTATGAGACGTGTGGTGATGATGACGTTTGCGGGGGTATTGCTATTGAGCCTGAGAAGGTCAGAAGGGATTAGGTTTGTGATAGACAGGGAAGAATGCTTCTCTCACAATGTTCAGTACGAGGGTGATACTGTTCATGTTTCTTTCGTCGTAATCAAGGCAGATTCGCCCTGGCACTACGGCGACGACGGTATAAAGGGCCCTTCTGGTGAACATCTTCATGATTTTCGTGACAAGACTAGTGAAAAGCATGAATTTATTGCTCACAACAAGGGGCTTCATCGATTCTGCTTCACGAACAAGTCTCCGTATCATGAAACCATAGACTTTGATGTGCACGTCGGTCACTTTTCGTATCATGAGCAGCATGCAAAAGATGAGCATTTCAACCCTTTGCTGGATCAAATATCTAAGTTGGAGGAAGCTCTTTACAATATTCAGTTTGAACAGCATTGGTTGGAGGCTCAGACTGACCGCCAGGCAATAGTGAATGATGGAATGAGCAGGAGGGCAATCCACAAGGCAATGTTTGAATCAGCAGCACTGGTAGGGGCCAGTGTCCTACAAGTCTACTTGCTGCAGCGCTTGTTTGAAAGGAAGCTTGGGACATCCAGAGTTTAA

Coding sequence (CDS)

ATGGAGCGATGGGCTATGAGACGTGTGGTGATGATGACGTTTGCGGGGGTATTGCTATTGAGCCTGAGAAGGTCAGAAGGGATTAGGTTTGTGATAGACAGGGAAGAATGCTTCTCTCACAATGTTCAGTACGAGGGTGATACTGTTCATGTTTCTTTCGTCGTAATCAAGGCAGATTCGCCCTGGCACTACGGCGACGACGGTATAAAGGGCCCTTCTGGTGAACATCTTCATGATTTTCGTGACAAGACTAGTGAAAAGCATGAATTTATTGCTCACAACAAGGGGCTTCATCGATTCTGCTTCACGAACAAGTCTCCGTATCATGAAACCATAGACTTTGATGTGCACGTCGGTCACTTTTCGTATCATGAGCAGCATGCAAAAGATGAGCATTTCAACCCTTTGCTGGATCAAATATCTAAGTTGGAGGAAGCTCTTTACAATATTCAGTTTGAACAGCATTGGTTGGAGGCTCAGACTGACCGCCAGGCAATAGTGAATGATGGAATGAGCAGGAGGGCAATCCACAAGGCAATGTTTGAATCAGCAGCACTGGTAGGGGCCAGTGTCCTACAAGTCTACTTGCTGCAGCGCTTGTTTGAAAGGAAGCTTGGGACATCCAGAGTTTAA

Protein sequence

MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADSPWHYGDDGIKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDVHVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAIHKAMFESAALVGASVLQVYLLQRLFERKLGTSRV

Homology

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BLAST of Sgr004975.1 vs. NCBI nr
Match: XP_038887290.1 (transmembrane emp24 domain-containing protein p24beta2 [Benincasa hispida])

HSP 1 Score: 412.9 bits (1060), Expect = 1.6e-111
Identity = 204/214 (95.33%), Postives = 206/214 (96.26%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAMR VVMM F GVLLL+LR SEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS
Sbjct: 1   MERWAMRFVVMMAFVGVLLLNLRESEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
           PWHYGDDG    IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV
Sbjct: 61  PWHYGDDGVDLVIKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMS+RAI
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSKRAI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 214

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BLAST of Sgr004975.1 vs. NCBI nr
Match: KAA0059209.1 (emp24/gp25L/p24 family protein [Cucumis melo var. makuwa] >TYK19311.1 emp24/gp25L/p24 family protein [Cucumis melo var. makuwa])

HSP 1 Score: 409.5 bits (1051), Expect = 1.8e-110
Identity = 201/214 (93.93%), Postives = 206/214 (96.26%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAMR VVMM F GVLLL+LR+SEGIRFVIDREECFSH VQYEGDTVHVSFVVIKADS
Sbjct: 1   MERWAMRFVVMMAFVGVLLLNLRKSEGIRFVIDREECFSHQVQYEGDTVHVSFVVIKADS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
           PWHYGDDG    IKGP+GEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV
Sbjct: 61  PWHYGDDGVDLVIKGPTGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPL+DQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMS+RAI
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLMDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSKRAI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 214

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BLAST of Sgr004975.1 vs. NCBI nr
Match: XP_004144551.1 (transmembrane emp24 domain-containing protein p24beta2 [Cucumis sativus] >KGN43402.1 hypothetical protein Csa_020232 [Cucumis sativus])

HSP 1 Score: 407.9 bits (1047), Expect = 5.2e-110
Identity = 200/214 (93.46%), Postives = 206/214 (96.26%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAMR V+MM F GVLLLSLR+SEGIRFVIDREECFSH+VQYEGDTVHVSFVVIKADS
Sbjct: 1   MERWAMRFVMMMAFVGVLLLSLRKSEGIRFVIDREECFSHHVQYEGDTVHVSFVVIKADS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
           PWHYGDDG    IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV
Sbjct: 61  PWHYGDDGVDLVIKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPL+DQISKLEEALYNIQFEQHWLEAQTDRQAIVN+GM +RAI
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLMDQISKLEEALYNIQFEQHWLEAQTDRQAIVNEGMGKRAI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 214

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BLAST of Sgr004975.1 vs. NCBI nr
Match: XP_022969260.1 (transmembrane emp24 domain-containing protein p24beta2 [Cucurbita maxima] >XP_022969261.1 transmembrane emp24 domain-containing protein p24beta2 [Cucurbita maxima])

HSP 1 Score: 405.6 bits (1041), Expect = 2.6e-109
Identity = 199/214 (92.99%), Postives = 205/214 (95.79%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAMRRVVMM FAGVLLL+LR+SEG+RFVIDREECFSHNVQY+GDTVHVSFVVIK DS
Sbjct: 1   MERWAMRRVVMMAFAGVLLLNLRQSEGLRFVIDREECFSHNVQYDGDTVHVSFVVIKTDS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
            WHYGDDG    IKGPSGEHLHDFRDK SEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV
Sbjct: 61  GWHYGDDGVDLVIKGPSGEHLHDFRDKASEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMS+RA+
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSKRAV 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKL TSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLRTSRV 214

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BLAST of Sgr004975.1 vs. NCBI nr
Match: XP_022136218.1 (transmembrane emp24 domain-containing protein p24beta2 [Momordica charantia])

HSP 1 Score: 404.8 bits (1039), Expect = 4.4e-109
Identity = 200/214 (93.46%), Postives = 204/214 (95.33%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAM    MM FAGV LLS+RRSEGIRFVIDREECFSHNVQY+GDTVHVSFVVIKADS
Sbjct: 1   MERWAM----MMAFAGVFLLSMRRSEGIRFVIDREECFSHNVQYDGDTVHVSFVVIKADS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
           PWHYGDDG    IKGPSGEHLHDFRDKTSEKHEF+AHNKG+HRFCFTNKSPYHETIDFDV
Sbjct: 61  PWHYGDDGVDLVIKGPSGEHLHDFRDKTSEKHEFVAHNKGIHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 210

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BLAST of Sgr004975.1 vs. ExPASy Swiss-Prot
Match: Q9S7M9 (Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana OX=3702 GN=At3g07680 PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 7.4e-83
Identity = 145/197 (73.60%), Postives = 172/197 (87.31%), Query Frame = 0


Query: 18  LLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADSPWHYGDDG----IKGPS 77
           LL S + + GIRFVIDREECFSH  +YEGDT+HVSFVVIK+DS WH+ +DG    I GP+
Sbjct: 12  LLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLVIHGPT 71

Query: 78  GEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDVHVGHFSYHEQHAKDEHF 137
           GE +HDFR++ S KH+F+   KG++RFCFTNKSPYHETIDFDV +GHF+Y++QHAKDEHF
Sbjct: 72  GEQIHDFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGHFAYYDQHAKDEHF 131

Query: 138 NPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAIHKAMFESAALVGASVLQ 197
            PL++QISKLEEALYNIQFEQHWLEAQTDRQAIVN+ MS+RA+HKA+FES AL+GAS LQ
Sbjct: 132 TPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESFALIGASFLQ 191

Query: 198 VYLLQRLFERKLGTSRV 211
           VYLL+RLFERKLG SRV
Sbjct: 192 VYLLRRLFERKLGMSRV 208

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BLAST of Sgr004975.1 vs. ExPASy Swiss-Prot
Match: Q9LIL4 (Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana OX=3702 GN=At3g22845 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-25
Identity = 73/214 (34.11%), Postives = 113/214 (52.80%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MER   +  V +    +LL S+ +   +   ++ EEC    V YEGDTV  +FVV+  D 
Sbjct: 1   MERRQAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDI 60

Query: 61  PW---HYG-DDGIKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
            W   H G D  +  P+G  +   +  + +K EF A   G+++FCF N     ET+ F +
Sbjct: 61  FWGSDHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYI 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGH       AKDEH +P+  +I++L EAL ++  EQ +L+A+  R    N+   +R I
Sbjct: 121 HVGHIPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
              + E   L  AS LQV  +++LF + +  +RV
Sbjct: 181 FYTVGEYIFLAAASGLQVLYIRKLFSKSVAYNRV 214

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BLAST of Sgr004975.1 vs. ExPASy TrEMBL
Match: A0A5D3D6W7 (Emp24/gp25L/p24 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00310 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 8.7e-111
Identity = 201/214 (93.93%), Postives = 206/214 (96.26%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAMR VVMM F GVLLL+LR+SEGIRFVIDREECFSH VQYEGDTVHVSFVVIKADS
Sbjct: 1   MERWAMRFVVMMAFVGVLLLNLRKSEGIRFVIDREECFSHQVQYEGDTVHVSFVVIKADS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
           PWHYGDDG    IKGP+GEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV
Sbjct: 61  PWHYGDDGVDLVIKGPTGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPL+DQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMS+RAI
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLMDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSKRAI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 214

[Close]

BLAST of Sgr004975.1 vs. ExPASy TrEMBL
Match: A0A0A0K541 (GOLD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G031630 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.5e-110
Identity = 200/214 (93.46%), Postives = 206/214 (96.26%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAMR V+MM F GVLLLSLR+SEGIRFVIDREECFSH+VQYEGDTVHVSFVVIKADS
Sbjct: 1   MERWAMRFVMMMAFVGVLLLSLRKSEGIRFVIDREECFSHHVQYEGDTVHVSFVVIKADS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
           PWHYGDDG    IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV
Sbjct: 61  PWHYGDDGVDLVIKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPL+DQISKLEEALYNIQFEQHWLEAQTDRQAIVN+GM +RAI
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLMDQISKLEEALYNIQFEQHWLEAQTDRQAIVNEGMGKRAI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 214

[Close]

BLAST of Sgr004975.1 vs. ExPASy TrEMBL
Match: A0A6J1HZG1 (transmembrane emp24 domain-containing protein p24beta2 OS=Cucurbita maxima OX=3661 GN=LOC111468315 PE=3 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.3e-109
Identity = 199/214 (92.99%), Postives = 205/214 (95.79%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAMRRVVMM FAGVLLL+LR+SEG+RFVIDREECFSHNVQY+GDTVHVSFVVIK DS
Sbjct: 1   MERWAMRRVVMMAFAGVLLLNLRQSEGLRFVIDREECFSHNVQYDGDTVHVSFVVIKTDS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
            WHYGDDG    IKGPSGEHLHDFRDK SEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV
Sbjct: 61  GWHYGDDGVDLVIKGPSGEHLHDFRDKASEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMS+RA+
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSKRAV 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKL TSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLRTSRV 214

[Close]

BLAST of Sgr004975.1 vs. ExPASy TrEMBL
Match: A0A6J1C720 (transmembrane emp24 domain-containing protein p24beta2 OS=Momordica charantia OX=3673 GN=LOC111007967 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.2e-109
Identity = 200/214 (93.46%), Postives = 204/214 (95.33%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAM    MM FAGV LLS+RRSEGIRFVIDREECFSHNVQY+GDTVHVSFVVIKADS
Sbjct: 1   MERWAM----MMAFAGVFLLSMRRSEGIRFVIDREECFSHNVQYDGDTVHVSFVVIKADS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
           PWHYGDDG    IKGPSGEHLHDFRDKTSEKHEF+AHNKG+HRFCFTNKSPYHETIDFDV
Sbjct: 61  PWHYGDDGVDLVIKGPSGEHLHDFRDKTSEKHEFVAHNKGIHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 210

[Close]

BLAST of Sgr004975.1 vs. ExPASy TrEMBL
Match: A0A6J1GLY5 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschata OX=3662 GN=LOC111455579 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.2e-107
Identity = 196/214 (91.59%), Postives = 203/214 (94.86%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MERWAMR VVMM FAGVLLL+LR+SEGIRFVIDREECFSHNVQY+GDTVHVSFVVIK DS
Sbjct: 1   MERWAMRCVVMMAFAGVLLLNLRQSEGIRFVIDREECFSHNVQYDGDTVHVSFVVIKTDS 60

Query: 61  PWHYGDDG----IKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
            WHYG+DG    IKGPSGEHLHD RDK SEKHEF+AHNKGLHRFCFTNKSPYHETIDFDV
Sbjct: 61  GWHYGEDGVDLVIKGPSGEHLHDIRDKASEKHEFVAHNKGLHRFCFTNKSPYHETIDFDV 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMS+RA+
Sbjct: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSKRAV 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
           HKAMFESAALVGASVLQVYLLQRLFERKL TSRV
Sbjct: 181 HKAMFESAALVGASVLQVYLLQRLFERKLRTSRV 214

[Close]

BLAST of Sgr004975.1 vs. TAIR 10
Match: AT3G07680.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 308.1 bits (788), Expect = 5.3e-84
Identity = 145/197 (73.60%), Postives = 172/197 (87.31%), Query Frame = 0


Query: 18  LLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADSPWHYGDDG----IKGPS 77
           LL S + + GIRFVIDREECFSH  +YEGDT+HVSFVVIK+DS WH+ +DG    I GP+
Sbjct: 12  LLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLVIHGPT 71

Query: 78  GEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDVHVGHFSYHEQHAKDEHF 137
           GE +HDFR++ S KH+F+   KG++RFCFTNKSPYHETIDFDV +GHF+Y++QHAKDEHF
Sbjct: 72  GEQIHDFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGHFAYYDQHAKDEHF 131

Query: 138 NPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAIHKAMFESAALVGASVLQ 197
            PL++QISKLEEALYNIQFEQHWLEAQTDRQAIVN+ MS+RA+HKA+FES AL+GAS LQ
Sbjct: 132 TPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESFALIGASFLQ 191

Query: 198 VYLLQRLFERKLGTSRV 211
           VYLL+RLFERKLG SRV
Sbjct: 192 VYLLRRLFERKLGMSRV 208

[Close]

BLAST of Sgr004975.1 vs. TAIR 10
Match: AT3G22845.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.3e-26
Identity = 73/214 (34.11%), Postives = 113/214 (52.80%), Query Frame = 0


Query: 1   MERWAMRRVVMMTFAGVLLLSLRRSEGIRFVIDREECFSHNVQYEGDTVHVSFVVIKADS 60
           MER   +  V +    +LL S+ +   +   ++ EEC    V YEGDTV  +FVV+  D 
Sbjct: 1   MERRQAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDI 60

Query: 61  PW---HYG-DDGIKGPSGEHLHDFRDKTSEKHEFIAHNKGLHRFCFTNKSPYHETIDFDV 120
            W   H G D  +  P+G  +   +  + +K EF A   G+++FCF N     ET+ F +
Sbjct: 61  FWGSDHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYI 120

Query: 121 HVGHFSYHEQHAKDEHFNPLLDQISKLEEALYNIQFEQHWLEAQTDRQAIVNDGMSRRAI 180
           HVGH       AKDEH +P+  +I++L EAL ++  EQ +L+A+  R    N+   +R I
Sbjct: 121 HVGHIPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVI 180

Query: 181 HKAMFESAALVGASVLQVYLLQRLFERKLGTSRV 211
              + E   L  AS LQV  +++LF + +  +RV
Sbjct: 181 FYTVGEYIFLAAASGLQVLYIRKLFSKSVAYNRV 214

The following BLAST results are available for this feature:

BLAST of Sgr004975.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887290.1	1.6e-111	95.33	transmembrane emp24 domain-containing protein p24beta2 [Benincasa hispida]	[more]
KAA0059209.1	1.8e-110	93.93	emp24/gp25L/p24 family protein [Cucumis melo var. makuwa] >TYK19311.1 emp24/gp25...	[more]
XP_004144551.1	5.2e-110	93.46	transmembrane emp24 domain-containing protein p24beta2 [Cucumis sativus] >KGN434...	[more]
XP_022969260.1	2.6e-109	92.99	transmembrane emp24 domain-containing protein p24beta2 [Cucurbita maxima] >XP_02...	[more]
XP_022136218.1	4.4e-109	93.46	transmembrane emp24 domain-containing protein p24beta2 [Momordica charantia]	[more]

BLAST of Sgr004975.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7M9	7.4e-83	73.60	Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana O...	[more]
Q9LIL4	1.9e-25	34.11	Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana O...	[more]

BLAST of Sgr004975.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D6W7	8.7e-111	93.93	Emp24/gp25L/p24 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A0A0K541	2.5e-110	93.46	GOLD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G031630 PE=3 S...	[more]
A0A6J1HZG1	1.3e-109	92.99	transmembrane emp24 domain-containing protein p24beta2 OS=Cucurbita maxima OX=36...	[more]
A0A6J1C720	2.2e-109	93.46	transmembrane emp24 domain-containing protein p24beta2 OS=Momordica charantia OX...	[more]
A0A6J1GLY5	1.2e-107	91.59	transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschat...	[more]

BLAST of Sgr004975.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07680.1	5.3e-84	73.60	emp24/gp25L/p24 family/GOLD family protein	[more]
AT3G22845.1	1.3e-26	34.11	emp24/gp25L/p24 family/GOLD family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009038	GOLD domain	SMART	SM01190	EMP24_GP25L_2	coord: 27..202 e-value: 6.8E-34 score: 128.6
IPR009038	GOLD domain	PFAM	PF01105	EMP24_GP25L	coord: 34..202 e-value: 2.3E-27 score: 96.1
IPR009038	GOLD domain	PROSITE	PS50866	GOLD	coord: 35..118 score: 13.567418
IPR015720	Transmembrane emp24 domain-containing protein	PANTHER	PTHR22811	TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN	coord: 6..206
None	No IPR available	PANTHER	PTHR22811:SF167	TMP21-RELATED PROTEIN-RELATED	coord: 6..206

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sgr004975	Sgr004975	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sgr004975.1.exon4	Sgr004975.1.exon4	exon
Sgr004975.1.exon3	Sgr004975.1.exon3	exon
Sgr004975.1.exon2	Sgr004975.1.exon2	exon
Sgr004975.1.exon1	Sgr004975.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Sgr004975.1	cds.Sgr004975.1_4	CDS
cds.Sgr004975.1	cds.Sgr004975.1_3	CDS
cds.Sgr004975.1	cds.Sgr004975.1_2	CDS
cds.Sgr004975.1	cds.Sgr004975.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sgr004975.1	Sgr004975.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport
biological_process	GO:0007030	Golgi organization
biological_process	GO:0006886	intracellular protein transport
cellular_component	GO:0030134	COPII-coated ER to Golgi transport vesicle
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005793	endoplasmic reticulum-Golgi intermediate compartment
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane