Sed0020675.2 (mRNA) Chayote v1

BLAST of Sed0020675.2 vs. NCBI nr
Match: KAG6606103.1 (putative copper-transporting ATPase HMA5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 739/840 (87.98%), Postives = 784/840 (93.33%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEENAKHV-------------EDGEAKTVVWVSGMSCSACAVSVEN 60
           M+KLPRRKRSPAATN+ENA  V             +  E K V+ VSGM+CSACAVSVEN
Sbjct: 1   MLKLPRRKRSPAATNQENANDVATIDDDETAAGARKAAEVKVVLCVSGMTCSACAVSVEN 60

Query: 61  SIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVC 120
           SIKHLPG+++AA+DFLNDRAQISYLPNLIDE+S++KAIENAGFQATISKD  +HRSREVC
Sbjct: 61  SIKHLPGILDAAIDFLNDRAQISYLPNLIDEDSIVKAIENAGFQATISKDSADHRSREVC 120

Query: 121 RIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIG 180
           RIRVNGMGC SCSSMVESVLEEMYGVQK HIAL N EAEVHYDPKVVNS QFIVAIQDIG
Sbjct: 121 RIRVNGMGCNSCSSMVESVLEEMYGVQKTHIALFNGEAEVHYDPKVVNSTQFIVAIQDIG 180

Query: 181 FDALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPD 240
           FDAL+ITIGEHISKIELKIDGM +ENS+ KVKESLESV GIED+DIDM L+KVTISY PD
Sbjct: 181 FDALLITIGEHISKIELKIDGMDNENSSTKVKESLESVVGIEDVDIDMGLTKVTISYKPD 240

Query: 241 ITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSM 300
           ITGPRTFIEVIESIKSEHFKATIYP   GRE RKEKEIKQHYKY LWS ALS+PVFLTSM
Sbjct: 241 ITGPRTFIEVIESIKSEHFKATIYPANVGRENRKEKEIKQHYKYFLWSSALSVPVFLTSM 300

Query: 301 VFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVL 360
           VFMYIPGIKQ LDIKVVNMMNVGH+I+WNLSTPVQFIIGSRFY+GSYKA+RRGSANMDVL
Sbjct: 301 VFMYIPGIKQILDIKVVNMMNVGHLIKWNLSTPVQFIIGSRFYIGSYKALRRGSANMDVL 360

Query: 361 ITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIA 420
           ITLGTNAAYFYSVYIVLR+ATSP F+GTDFFETSSMLITFILLGKYLE+LAKGKTSEAIA
Sbjct: 361 ITLGTNAAYFYSVYIVLRAATSPAFDGTDFFETSSMLITFILLGKYLEILAKGKTSEAIA 420

Query: 421 KLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVN 480
           KLK+LAPETATLLTLD HG+VI E EI+SELIQKNDVIKI PG +VASDG+VVWGESHVN
Sbjct: 421 KLKHLAPETATLLTLDGHGNVISEVEISSELIQKNDVIKITPGAKVASDGIVVWGESHVN 480

Query: 481 ESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPI 540
           ESMITGEAKPVAKRTGDKVIGGT+NENGVLHIK THVGSESSLAQIVRLVESSQLAKAPI
Sbjct: 481 ESMITGEAKPVAKRTGDKVIGGTMNENGVLHIKATHVGSESSLAQIVRLVESSQLAKAPI 540

Query: 541 QKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600
           QK ADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI
Sbjct: 541 QKLADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600

Query: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVN 660
           ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHK SCIVFDKTGTLT+GKPVVVN
Sbjct: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKASCIVFDKTGTLTVGKPVVVN 660

Query: 661 VELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGV 720
           V+LM+ +VLEELLELTAATEVNSEHP+AKAIVEYAKQ KEE NPIWPEAQEFISIPGHGV
Sbjct: 661 VKLMDTIVLEELLELTAATEVNSEHPIAKAIVEYAKQFKEEHNPIWPEAQEFISIPGHGV 720

Query: 721 EATVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDP 780
           EA VRNKK++VGNKSLMMNN IEI GE ESFL+DAEGMA+TAVLVA++RTVSGVI V+DP
Sbjct: 721 EAIVRNKKVVVGNKSLMMNNGIEILGETESFLVDAEGMAKTAVLVAVDRTVSGVIVVSDP 780

Query: 781 LKPGSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           LKPG+KEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT
Sbjct: 781 LKPGAKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 840

BLAST of Sed0020675.2 vs. NCBI nr
Match: XP_023534764.1 (probable copper-transporting ATPase HMA5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 741/840 (88.21%), Postives = 784/840 (93.33%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEENAKHV-------------EDGEAKTVVWVSGMSCSACAVSVEN 60
           M+KLPRRKRSPAATN+ENA  V             +  EAK V+ VSGM+CSACAVSVEN
Sbjct: 1   MLKLPRRKRSPAATNQENANDVATIDNDETAAGARKAVEAKVVLCVSGMTCSACAVSVEN 60

Query: 61  SIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVC 120
           SIKHLPG+++AA+DFLNDRAQI YLPNLIDE+S++KAIENAGFQATISKD  +HRSREVC
Sbjct: 61  SIKHLPGILDAAIDFLNDRAQIRYLPNLIDEDSIVKAIENAGFQATISKDSADHRSREVC 120

Query: 121 RIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIG 180
           RIRVNGMGC SCSSMVESVLEEMYGVQK HIAL N EAEVHYDPKVVNS QFI+AIQDIG
Sbjct: 121 RIRVNGMGCNSCSSMVESVLEEMYGVQKTHIALFNGEAEVHYDPKVVNSTQFIIAIQDIG 180

Query: 181 FDALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPD 240
           FDAL+ITIGEHISKIELKIDGM + NS+ KVKESLESV GIEDIDIDM L+KVTISY PD
Sbjct: 181 FDALLITIGEHISKIELKIDGMDNGNSSTKVKESLESVVGIEDIDIDMGLTKVTISYKPD 240

Query: 241 ITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSM 300
           ITGPRTFIEVIESIKSEHFKATIYP   GRE RKEKEIKQHYKY LWS ALS+PVFLTSM
Sbjct: 241 ITGPRTFIEVIESIKSEHFKATIYPANVGRENRKEKEIKQHYKYFLWSSALSVPVFLTSM 300

Query: 301 VFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVL 360
           VFMYIPGIKQ LDIKVVNMMNVGH+I+WNLSTPVQFIIGSRFY+GSYKA+RRGSANMDVL
Sbjct: 301 VFMYIPGIKQILDIKVVNMMNVGHLIKWNLSTPVQFIIGSRFYIGSYKALRRGSANMDVL 360

Query: 361 ITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIA 420
           ITLGTNAAYFYSVYIVLR+ATSP F+GTDFFETSSMLITFILLGKYLE+LAKGKTSEAIA
Sbjct: 361 ITLGTNAAYFYSVYIVLRAATSPAFDGTDFFETSSMLITFILLGKYLEILAKGKTSEAIA 420

Query: 421 KLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVN 480
           KLK+LAPETATLLTLD HG+VI E EI+SELIQKNDVIKI PG +VASDGLVVWGESHVN
Sbjct: 421 KLKHLAPETATLLTLDGHGNVISEVEISSELIQKNDVIKITPGAKVASDGLVVWGESHVN 480

Query: 481 ESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPI 540
           ESMITGEAKPVAKRTGDKVIGGT+NENGVLHIK THVGSESSLAQIVRLVESSQLAKAPI
Sbjct: 481 ESMITGEAKPVAKRTGDKVIGGTMNENGVLHIKATHVGSESSLAQIVRLVESSQLAKAPI 540

Query: 541 QKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600
           QK ADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI
Sbjct: 541 QKLADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600

Query: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVN 660
           ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHK SCIVFDKTGTLTIGKPVVVN
Sbjct: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKASCIVFDKTGTLTIGKPVVVN 660

Query: 661 VELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGV 720
           V+LM+ +VLEELLELTAATEVNSEHP+AKAIVEYAKQLKEE NPIWPEAQEFISIPGHGV
Sbjct: 661 VKLMDTIVLEELLELTAATEVNSEHPIAKAIVEYAKQLKEEHNPIWPEAQEFISIPGHGV 720

Query: 721 EATVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDP 780
           EA VRNKK++VGNKSLMMNN IEI GE ESFL+DAEGMA+TAVLVA++RTVSGVI V+DP
Sbjct: 721 EAIVRNKKVVVGNKSLMMNNGIEILGETESFLVDAEGMAKTAVLVAVDRTVSGVIVVSDP 780

Query: 781 LKPGSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           LKPG+KEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT
Sbjct: 781 LKPGAKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 840

BLAST of Sed0020675.2 vs. NCBI nr
Match: XP_023521632.1 (probable copper-transporting ATPase HMA5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1425.2 bits (3688), Expect = 0.0e+00
Identity = 737/840 (87.74%), Postives = 782/840 (93.10%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEENAKHV-------------EDGEAKTVVWVSGMSCSACAVSVEN 60
           M+KLPRRKRSPAATN+ENA  V             +  E K V+ VSGM+CSACAVSVEN
Sbjct: 1   MLKLPRRKRSPAATNQENANDVATIVNDETAAGARKAVETKVVLCVSGMTCSACAVSVEN 60

Query: 61  SIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVC 120
           SIKHLPG+++AA+DFLNDRAQI YLPNLIDE+S++KAIENAGFQATISKD  +HRSREVC
Sbjct: 61  SIKHLPGILDAAIDFLNDRAQIRYLPNLIDEDSIVKAIENAGFQATISKDSADHRSREVC 120

Query: 121 RIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIG 180
           RIRVNGMGC SCSSMVESVLEEMYGVQK HIAL N EAEVHYDPKVVNS QFI+AIQDIG
Sbjct: 121 RIRVNGMGCNSCSSMVESVLEEMYGVQKTHIALFNGEAEVHYDPKVVNSTQFIIAIQDIG 180

Query: 181 FDALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPD 240
           FDAL+ITIGEHISKIELKIDGM + NS+ KVKESLESV GIEDIDIDM L+KVTISY PD
Sbjct: 181 FDALLITIGEHISKIELKIDGMDNGNSSTKVKESLESVVGIEDIDIDMGLTKVTISYKPD 240

Query: 241 ITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSM 300
           ITGPRTFIEVIESIKSEHFKATIYP   GRE RKEKEIKQHYKY LWS ALS+PVFLTSM
Sbjct: 241 ITGPRTFIEVIESIKSEHFKATIYPANVGRENRKEKEIKQHYKYFLWSSALSVPVFLTSM 300

Query: 301 VFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVL 360
           VFMYIPGIKQ LDIKVVNMMNVGH+I+WNLSTPVQFIIGSRFY+GSYKA+RRGSANMDVL
Sbjct: 301 VFMYIPGIKQILDIKVVNMMNVGHLIKWNLSTPVQFIIGSRFYIGSYKALRRGSANMDVL 360

Query: 361 ITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIA 420
           ITLGTNAAYFYSVYIVLR+ATSP F+GTDFFETSSMLITFILLGKYLE+LAKGKTSEAIA
Sbjct: 361 ITLGTNAAYFYSVYIVLRAATSPAFDGTDFFETSSMLITFILLGKYLEILAKGKTSEAIA 420

Query: 421 KLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVN 480
           KLK+LAPETATLLTLD HG+VI E EI+SELIQKNDVIKI PG +VASDGLVVWGESHVN
Sbjct: 421 KLKHLAPETATLLTLDGHGNVISEVEISSELIQKNDVIKITPGAKVASDGLVVWGESHVN 480

Query: 481 ESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPI 540
           ESMITGEAKPVAKRTGDKVIGGT+NENGVLHIK THVGSESSLAQIVRLVESSQLAKAPI
Sbjct: 481 ESMITGEAKPVAKRTGDKVIGGTMNENGVLHIKATHVGSESSLAQIVRLVESSQLAKAPI 540

Query: 541 QKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600
           QK ADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI
Sbjct: 541 QKLADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600

Query: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVN 660
           ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHK SCIVFDKTGTLTIGKPVVVN
Sbjct: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKASCIVFDKTGTLTIGKPVVVN 660

Query: 661 VELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGV 720
           V+LM+ +VLEELLELTAATEVNSEHP+AKAIVEYAKQ KEE NPIWPEAQEFISIPGHGV
Sbjct: 661 VKLMDTIVLEELLELTAATEVNSEHPIAKAIVEYAKQFKEEHNPIWPEAQEFISIPGHGV 720

Query: 721 EATVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDP 780
           EA +RNKK++VGNKSLMMNN IEI GE ESFL+DAEGMA+TAVLVA++RTVSGVI V+DP
Sbjct: 721 EAIIRNKKVVVGNKSLMMNNGIEILGETESFLVDAEGMAKTAVLVAVDRTVSGVIVVSDP 780

Query: 781 LKPGSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           LKPG+KEVISILKS+EVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT
Sbjct: 781 LKPGAKEVISILKSLEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 840

BLAST of Sed0020675.2 vs. NCBI nr
Match: XP_022958144.1 (probable copper-transporting ATPase HMA5 [Cucurbita moschata])

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 737/840 (87.74%), Postives = 783/840 (93.21%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEENAKHVED-------------GEAKTVVWVSGMSCSACAVSVEN 60
           M+KLPRRKRSPAATN+ENA  V +              EAK V+ VSGM+CSACAVSVEN
Sbjct: 1   MLKLPRRKRSPAATNQENANDVANIDNDETAAGVRKAAEAKVVLCVSGMTCSACAVSVEN 60

Query: 61  SIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVC 120
           SIKHLPG+++AA+DFLNDRAQI YLPNLIDE+S++KAIENAGFQATISKD  +HRSREVC
Sbjct: 61  SIKHLPGILDAAIDFLNDRAQIRYLPNLIDEDSIVKAIENAGFQATISKDSADHRSREVC 120

Query: 121 RIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIG 180
           RIRVNGMGC SCSSMVESVLEEMYGVQK HIAL N EAEVHYDPKVVNS QFIVAIQDIG
Sbjct: 121 RIRVNGMGCNSCSSMVESVLEEMYGVQKTHIALFNGEAEVHYDPKVVNSTQFIVAIQDIG 180

Query: 181 FDALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPD 240
           FDAL+ITIGEHISKIELKIDGMH+ENS+ KVKESLESV GIED+DIDM L+KVTISY PD
Sbjct: 181 FDALLITIGEHISKIELKIDGMHNENSSTKVKESLESVVGIEDVDIDMGLTKVTISYKPD 240

Query: 241 ITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSM 300
           ITGPRTFIEVIESIKSEHFKATIYP   GRE RKEKEIKQHYKY LWS ALS+PVFLTSM
Sbjct: 241 ITGPRTFIEVIESIKSEHFKATIYPANVGRENRKEKEIKQHYKYFLWSSALSVPVFLTSM 300

Query: 301 VFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVL 360
           VFMYIPGIKQ LDIKVVNMMNVGH+I+WNLSTPVQFIIGSRFY+GSYKA+RRGSANMDVL
Sbjct: 301 VFMYIPGIKQILDIKVVNMMNVGHLIKWNLSTPVQFIIGSRFYIGSYKALRRGSANMDVL 360

Query: 361 ITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIA 420
           ITLGTNAAYFYSVYIVLR+ATSP F+GTDFFETSSMLITFILLGKYLE+LAKGKTSEAIA
Sbjct: 361 ITLGTNAAYFYSVYIVLRAATSPAFDGTDFFETSSMLITFILLGKYLEILAKGKTSEAIA 420

Query: 421 KLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVN 480
           KLK+LAPETATLLTLD HG+VI E EI+ ELIQKNDVIKI PG +VASDGLVVWGESHVN
Sbjct: 421 KLKHLAPETATLLTLDGHGNVISEVEISGELIQKNDVIKITPGAKVASDGLVVWGESHVN 480

Query: 481 ESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPI 540
           ESMITGEAKPVAKRTGDKVIGGT+NENGVLHIK THVGSESSLAQIV+LVESSQLAKAPI
Sbjct: 481 ESMITGEAKPVAKRTGDKVIGGTMNENGVLHIKATHVGSESSLAQIVQLVESSQLAKAPI 540

Query: 541 QKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600
           QK ADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMD FELALQFGISVMVI
Sbjct: 541 QKLADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDRFELALQFGISVMVI 600

Query: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVN 660
           ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHK SCIVFDKTGTLT+GKPVVVN
Sbjct: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKASCIVFDKTGTLTVGKPVVVN 660

Query: 661 VELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGV 720
           V+LM+ +VLEELLELTAATEVNSEHP+AKAIVEYAKQ KE+ NPIWPEAQEFISIPGHGV
Sbjct: 661 VKLMDTIVLEELLELTAATEVNSEHPIAKAIVEYAKQFKED-NPIWPEAQEFISIPGHGV 720

Query: 721 EATVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDP 780
           EA VRNKK++VGNKSLMMNN IEI GE ESFL+DAEGMA+TAVLVA++RTVSGVI V+DP
Sbjct: 721 EAIVRNKKVVVGNKSLMMNNGIEILGETESFLVDAEGMAKTAVLVAVDRTVSGVIVVSDP 780

Query: 781 LKPGSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           LKPG+KEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT
Sbjct: 781 LKPGAKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 839

BLAST of Sed0020675.2 vs. NCBI nr
Match: KAG7036049.1 (putative copper-transporting ATPase HMA5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 736/842 (87.41%), Postives = 781/842 (92.76%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEENAKHV---------------EDGEAKTVVWVSGMSCSACAVSV 60
           M+KLPRRKRSPAATN+ENA  V               +  E K V+ VSGM+CSACAVSV
Sbjct: 1   MLKLPRRKRSPAATNQENANDVATIDDDETAAGAGARKAAEVKVVLCVSGMTCSACAVSV 60

Query: 61  ENSIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSRE 120
           ENSIKHLPG+++AA+DFLNDRAQI YLPNLIDE+S++KAIENAGFQATISKD  +HRSRE
Sbjct: 61  ENSIKHLPGILDAAIDFLNDRAQIRYLPNLIDEDSIVKAIENAGFQATISKDSADHRSRE 120

Query: 121 VCRIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQD 180
           VCRIRVNGMGC SCSSMVESVLEEMYGVQK HIAL N EAEVHYDPKVVNS QFIVAIQD
Sbjct: 121 VCRIRVNGMGCNSCSSMVESVLEEMYGVQKTHIALFNGEAEVHYDPKVVNSTQFIVAIQD 180

Query: 181 IGFDALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYW 240
           IGFDAL+ITIGEHISKIELKIDGM +ENS+ KVKESLESV GIED+DIDM L+KVTISY 
Sbjct: 181 IGFDALLITIGEHISKIELKIDGMDNENSSTKVKESLESVVGIEDVDIDMGLTKVTISYK 240

Query: 241 PDITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLT 300
           PDITGPRTFIEVIESIKSEHFKATIYP   GRE  KEKEIKQHYKY LWS ALS+PVFLT
Sbjct: 241 PDITGPRTFIEVIESIKSEHFKATIYPANVGRENHKEKEIKQHYKYFLWSSALSVPVFLT 300

Query: 301 SMVFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMD 360
           SMVFMYIPGIKQ LDIKVVNMMNVGH+I+WNLSTPVQFIIGSRFY+GSYKA+RRGSANMD
Sbjct: 301 SMVFMYIPGIKQILDIKVVNMMNVGHLIKWNLSTPVQFIIGSRFYIGSYKALRRGSANMD 360

Query: 361 VLITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEA 420
           VLITLGTNAAYFYSVYIVLR+ATSP F+GTDFFETSSMLITFILLGKYLE+LAKGKTSEA
Sbjct: 361 VLITLGTNAAYFYSVYIVLRAATSPAFDGTDFFETSSMLITFILLGKYLEILAKGKTSEA 420

Query: 421 IAKLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESH 480
           IAKLK+LAPETATLLTLD HG+VI E EI+SELIQKNDVIKI PG +VASDG+VVWGESH
Sbjct: 421 IAKLKHLAPETATLLTLDGHGNVISEVEISSELIQKNDVIKITPGAKVASDGIVVWGESH 480

Query: 481 VNESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKA 540
           VNESMITGEAKPVAKRTGDKVIGGT+NENGVLHIK THVGSESSLAQIVRLVE SQLAKA
Sbjct: 481 VNESMITGEAKPVAKRTGDKVIGGTMNENGVLHIKATHVGSESSLAQIVRLVELSQLAKA 540

Query: 541 PIQKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVM 600
           PIQK ADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVM
Sbjct: 541 PIQKLADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVM 600

Query: 601 VIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVV 660
           VIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHK SCIVFDKTGTLT+GKPVV
Sbjct: 601 VIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKASCIVFDKTGTLTVGKPVV 660

Query: 661 VNVELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGH 720
           VNV+LM+ +VLEELLELTAATEVNSEHP+AKAIVEYAKQ KEE NPIWPEAQEFISIPGH
Sbjct: 661 VNVKLMDTIVLEELLELTAATEVNSEHPIAKAIVEYAKQFKEEHNPIWPEAQEFISIPGH 720

Query: 721 GVEATVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVT 780
           GVEA VRNKK++VGNKSLMMNN IEI GE ESFL+DAEGMA+TAVLVA++RTVSGVI V+
Sbjct: 721 GVEAIVRNKKVVVGNKSLMMNNGIEILGETESFLVDAEGMAKTAVLVAVDRTVSGVIVVS 780

Query: 781 DPLKPGSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNL 828
           DPLKPG+KEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNL
Sbjct: 781 DPLKPGAKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNL 840

BLAST of Sed0020675.2 vs. ExPASy Swiss-Prot
Match: Q9SH30 (Probable copper-transporting ATPase HMA5 OS=Arabidopsis thaliana OX=3702 GN=HMA5 PE=1 SV=2)

HSP 1 Score: 1069.7 bits (2765), Expect = 1.7e-311
Identity = 544/819 (66.42%), Postives = 679/819 (82.91%), Query Frame = 0

Query: 13  ATNEENAKHVEDGEAKTVVWVSGMSCSACAVSVENSIKHLPGVINAAVDFLNDRAQISYL 72
           +++E  A  ++D  ++ V  V GM+CSACA SVE +IK LPG+ +A +D LN+RAQI + 
Sbjct: 37  SSSETAAFEIDDPISRAVFQVLGMTCSACAGSVEKAIKRLPGIHDAVIDALNNRAQILFY 96

Query: 73  PNLIDEESLLKAIENAGFQATISKDGTNHRSREVCRIRVNGMGCKSCSSMVESVLEEMYG 132
           PN +D E++ + IE+AGF+A++ ++  N RSR+VCRIR+NGM C SCSS +E VL+ + G
Sbjct: 97  PNSVDVETIRETIEDAGFEASLIENEANERSRQVCRIRINGMTCTSCSSTIERVLQSVNG 156

Query: 133 VQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIGFDALIITIGEHISKIELKIDGMHSE 192
           VQ+AH+AL  EEAE+HYDP++ + ++ +  I++ GF+A++I+ GE +SKI+LKIDG  ++
Sbjct: 157 VQRAHVALAIEEAEIHYDPRLSSYDRLLEEIENAGFEAVLISTGEDVSKIDLKIDGELTD 216

Query: 193 NSTIKVKESLESVPGIEDIDIDMALSKVTISYWPDITGPRTFIEVIESI---KSEHFKAT 252
            S   ++ SLE++PG++ ++I     K+++ Y PD+TGPR FI+VIES     S H KAT
Sbjct: 217 ESMKVIERSLEALPGVQSVEISHGTDKISVLYKPDVTGPRNFIQVIESTVFGHSGHIKAT 276

Query: 253 IYPE-EAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSMVFMYIPGIKQTLDIKVVNMMN 312
           I+ E   GRE++K+ EIKQ+YK  LWS   ++PVFLT+MVFMYIPGIK  L  KV+NM+ 
Sbjct: 277 IFSEGGVGRESQKQGEIKQYYKSFLWSLVFTVPVFLTAMVFMYIPGIKDLLMFKVINMLT 336

Query: 313 VGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSAT 372
           VG IIR  L+TPVQF+IG RFY GSYKA+RRGSANMDVLI LGTNAAYFYS+Y VLR+AT
Sbjct: 337 VGEIIRCVLATPVQFVIGWRFYTGSYKALRRGSANMDVLIALGTNAAYFYSLYTVLRAAT 396

Query: 373 SPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIAKLKNLAPETATLLTLDDHGDV 432
           SP F G DFFETS+MLI+FI+LGKYLEV+AKGKTS+AIAKL NLAP+TA LL+LD  G+V
Sbjct: 397 SPDFKGVDFFETSAMLISFIILGKYLEVMAKGKTSQAIAKLMNLAPDTAILLSLDKEGNV 456

Query: 433 IGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVIG 492
            GE EI   LIQKNDVIKI+PG +VASDG V+WG+SHVNESMITGEA+PVAKR GD VIG
Sbjct: 457 TGEEEIDGRLIQKNDVIKIVPGAKVASDGYVIWGQSHVNESMITGEARPVAKRKGDTVIG 516

Query: 493 GTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSFL 552
           GT+NENGVLH+KVT VGSES+LAQIVRLVES+QLAKAP+QK AD ISK+FVPLVI LSF 
Sbjct: 517 GTLNENGVLHVKVTRVGSESALAQIVRLVESAQLAKAPVQKLADRISKFFVPLVIFLSFS 576

Query: 553 TWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVG 612
           TW+AWFLAGKLH YP+SW+PSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVG
Sbjct: 577 TWLAWFLAGKLHWYPESWIPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVG 636

Query: 613 ASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVELMNNVVLEELLELTAATEV 672
           ASQGVLIKGGQALE AHKV+CIVFDKTGTLT+GKPVVV  +L+ N+VL E  EL AATEV
Sbjct: 637 ASQGVLIKGGQALERAHKVNCIVFDKTGTLTMGKPVVVKTKLLKNMVLREFYELVAATEV 696

Query: 673 NSEHPVAKAIVEYAKQLK-EEQNPIWPEAQEFISIPGHGVEATVRNKKIIVGNKSLMMNN 732
           NSEHP+AKAIVEYAK+ + +E+NP WPEA +F+SI G GV+ATV+ ++I+VGNK+LM ++
Sbjct: 697 NSEHPLAKAIVEYAKKFRDDEENPAWPEACDFVSITGKGVKATVKGREIMVGNKNLMNDH 756

Query: 733 DIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSKEVISILKSMEVKSI 792
            + IP +AE  L D+E MAQT +LV+IN  + GV++V+DPLKP ++E ISILKSM +KSI
Sbjct: 757 KVIIPDDAEELLADSEDMAQTGILVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSI 816

Query: 793 MVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQ 827
           MVTGDNWGTANSIA+EVGI++VIAEAKP+QKAE+VK LQ
Sbjct: 817 MVTGDNWGTANSIAREVGIDSVIAEAKPEQKAEKVKELQ 855

BLAST of Sed0020675.2 vs. ExPASy Swiss-Prot
Match: A3AWA4 (Copper-transporting ATPase HMA5 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA5 PE=2 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 2.7e-293
Identity = 513/838 (61.22%), Postives = 652/838 (77.80%), Query Frame = 0

Query: 4   LPRRKRSPAATNEEN--------------AKHVEDGEAKTVVWVSGMSCSACAVSVENSI 63
           +PRR RS A   E                A   E+ E   V  VSGM+C+ACA SVE ++
Sbjct: 38  MPRRPRSAAVAGEGGEGGGGGGDGDLEAAAVGAEEEEKVAVFEVSGMTCAACAGSVEKAV 97

Query: 64  KHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVCRI 123
           K L G+ +AAVD L  RAQ+ + P  + EE + + I++ GF+A +  +    ++  VCR+
Sbjct: 98  KRLQGIHDAAVDVLGGRAQVVFYPAFVSEEKIRETIQDVGFEAKLIDEEVKEKNILVCRL 157

Query: 124 RVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIGFD 183
            + GM C SC+S VES+L+ + GVQ+A +AL  EEAE+ YD ++V ++Q   A+++ GF+
Sbjct: 158 HIKGMTCTSCASTVESILQVVPGVQRASVALATEEAEIRYDRRIVTASQLTHAVEETGFE 217

Query: 184 ALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPDIT 243
           A++IT G+  S+I+LK+DG  +E S + VK S++++PG+EDI +D  L K+TISY PD T
Sbjct: 218 AILITTGDDQSRIDLKVDGTLNERSIMIVKSSVQALPGVEDIKVDPELHKITISYKPDQT 277

Query: 244 GPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSMVF 303
           GPR  IEVIES  S     +IYPE  GR+  +  EIK++ +  LWS   +IPVFLTSMVF
Sbjct: 278 GPRDLIEVIESAASGDLTVSIYPEADGRQQHRHGEIKRYRQSFLWSLVFTIPVFLTSMVF 337

Query: 304 MYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVLIT 363
           MYIPG+K  L+ KV+NMM++G ++RW LSTPVQF+IG RFY G+YKA+  GS+NMDVLI 
Sbjct: 338 MYIPGLKDGLEKKVINMMSIGELLRWILSTPVQFVIGRRFYTGAYKALSHGSSNMDVLIA 397

Query: 364 LGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIAKL 423
           LGTN AYFYSVY +LR+A+S  +  TDFFETSSMLI+FILLGKYLE+LAKGKTSEAIAKL
Sbjct: 398 LGTNTAYFYSVYSILRAASSHNYMATDFFETSSMLISFILLGKYLEILAKGKTSEAIAKL 457

Query: 424 KNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVNES 483
            +LAPETAT+L  D  G+V+GE EI S LIQKNDVIK++PGG+VASDG V+WG+SHVNES
Sbjct: 458 MDLAPETATMLIYDHEGNVVGEKEIDSRLIQKNDVIKVVPGGKVASDGFVIWGQSHVNES 517

Query: 484 MITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPIQK 543
           MITGE++PVAKR GD VIGGTVNENGVLH++ T VGSES+LAQIVRLVES+Q+AKAP+QK
Sbjct: 518 MITGESRPVAKRKGDTVIGGTVNENGVLHVRATFVGSESALAQIVRLVESAQMAKAPVQK 577

Query: 544 FADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIAC 603
           FAD IS+ FVPLVI+LS LTW+AWFLAG+LH YP SW+PSSMDSF+LALQFGISVMVIAC
Sbjct: 578 FADQISRVFVPLVIILSLLTWLAWFLAGRLHGYPNSWIPSSMDSFQLALQFGISVMVIAC 637

Query: 604 PCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVE 663
           PCALGLATPTAVMV TGVGASQGVLIKGGQALE A KV CIVFDKTGTLTIGKPVVVN  
Sbjct: 638 PCALGLATPTAVMVATGVGASQGVLIKGGQALESAQKVDCIVFDKTGTLTIGKPVVVNTR 697

Query: 664 LMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGVEA 723
           L+ N+VL E     AA EVNSEHP+ KA+VE+AK+   E++ +W EA++FIS+ GHGV+A
Sbjct: 698 LLKNMVLREFYAYVAAAEVNSEHPLGKAVVEHAKKFHSEESHVWTEARDFISVTGHGVKA 757

Query: 724 TVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLK 783
            +  + ++VGNKS M+ + I+IP EA   L + E  AQTA++VA+++ V G+I+V+DP+K
Sbjct: 758 KISGRAVMVGNKSFMLTSGIDIPVEALEILTEEEEKAQTAIIVAMDQEVVGIISVSDPIK 817

Query: 784 PGSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           P ++EVIS LKSM+V+SIMVTGDNWGTAN+I+KEVGIE  +AEAKP+QKAE+VK LQ+
Sbjct: 818 PNAREVISYLKSMKVESIMVTGDNWGTANAISKEVGIENTVAEAKPEQKAEKVKELQS 875

BLAST of Sed0020675.2 vs. ExPASy Swiss-Prot
Match: Q6H7M3 (Copper-transporting ATPase HMA4 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA4 PE=1 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 2.2e-239
Identity = 429/804 (53.36%), Postives = 577/804 (71.77%), Query Frame = 0

Query: 28  KTVVWVSGMSCSACAVSVENSIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIEN 87
           K +  V G+SC++CAVS+E  +  L GV + +V  L  +A + Y P   D  ++ +AIE 
Sbjct: 38  KVMFNVRGISCASCAVSIETVVAGLKGVESVSVSPLQGQAVVQYRPEEADARTIKEAIEG 97

Query: 88  AGFQATISKDGTNHRSREVCRIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEV 147
             F+     D    +   VCR+++ GM C SCS  VE  L+ + GV+KA + L  EEA+V
Sbjct: 98  LNFEV----DELQEQEIAVCRLQIKGMACTSCSESVERALQMVPGVKKAAVGLALEEAKV 157

Query: 148 HYDPKVVNSNQFIVAIQDIGFDALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPG 207
           H+DP + + +  I AI+D GF A +I+ G+ ++K+ LK++G+ S      ++  LESV G
Sbjct: 158 HFDPNITSRDLIIEAIEDAGFGADLISSGDDVNKVHLKLEGVSSPEDIKLIQSRLESVEG 217

Query: 208 IEDIDIDMALSKVTISYWPDITGPRTFIEVIESIKS--EHFKATIYPEEAGRETRKEKEI 267
           + +++ D A   + ++Y PD+TGPR  I+ I+      ++F A++Y     RE  +  EI
Sbjct: 218 VNNVECDTAGQTIIVAYDPDVTGPRLLIQCIQDAAQPPKYFNASLYSPPKQREAERHHEI 277

Query: 268 KQHYKYLLWSCALSIPVFLTSMVFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFII 327
           + +    LWSC  S+PVF+ SMV   I      L  KV N M +G ++RW L +PVQFII
Sbjct: 278 RNYRNQFLWSCLFSVPVFMFSMVLPMISPFGDWLFYKVCNNMTIGMLLRWLLCSPVQFII 337

Query: 328 GSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLI 387
           G RFYVG+Y A++RG +NMDVL+ LGTNAAYFYSVYIVL++ TS  F G DFFETS+MLI
Sbjct: 338 GWRFYVGAYHALKRGYSNMDVLVALGTNAAYFYSVYIVLKALTSESFEGQDFFETSAMLI 397

Query: 388 TFILLGKYLEVLAKGKTSEAIAKLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVI 447
           +FILLGKYLEV+AKGKTS+A++KL  LAPETA LLTLD  G+ I E EI+++L+Q+NDVI
Sbjct: 398 SFILLGKYLEVVAKGKTSDALSKLTELAPETACLLTLDKDGNAISETEISTQLLQRNDVI 457

Query: 448 KIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVG 507
           KI+PG +V  DG+V+ G+SHVNESMITGEA+P+AK+ GDKVIGGTVN+NG + +KVTHVG
Sbjct: 458 KIVPGEKVPVDGVVIKGQSHVNESMITGEARPIAKKPGDKVIGGTVNDNGCIIVKVTHVG 517

Query: 508 SESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKS 567
           SE++L+QIV+LVE++QLA+AP+QK AD IS++FVP V++ +FLTW+ WF+AG+  +YP+ 
Sbjct: 518 SETALSQIVQLVEAAQLARAPVQKLADRISRFFVPTVVVAAFLTWLGWFVAGQFDIYPRE 577

Query: 568 WLPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAH 627
           W+P +MDSFELALQFGISV+V+ACPCALGLATPTAVMV TG GASQGVLIKGG ALE AH
Sbjct: 578 WIPKAMDSFELALQFGISVLVVACPCALGLATPTAVMVATGKGASQGVLIKGGNALEKAH 637

Query: 628 KVSCIVFDKTGTLTIGKPVVVNVELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQL 687
           KV  I+FDKTGTLT+GKP VV  ++ + + L EL +L A  E NSEHP++KAIVEY K+L
Sbjct: 638 KVKAIIFDKTGTLTVGKPSVVQTKVFSKIPLLELCDLAAGAEANSEHPLSKAIVEYTKKL 697

Query: 688 KEE---QNPIWPEAQEFISIPGHGVEATVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDA 747
           +E+    +    E+++F   PG GV A V  K ++VGNK LM   ++ I  E E  + + 
Sbjct: 698 REQYGSHSDHIMESKDFEVHPGAGVSANVEGKLVLVGNKRLMQEFEVPISSEVEGHMSET 757

Query: 748 EGMAQTAVLVAINRTVSGVITVTDPLKPGSKEVISILKSMEVKSIMVTGDNWGTANSIAK 807
           E +A+T VLVAI+RT+ G ++V+DPLKP +   IS L SM + SIMVTGDNW TA SIAK
Sbjct: 758 EELARTCVLVAIDRTICGALSVSDPLKPEAGRAISYLSSMGISSIMVTGDNWATAKSIAK 817

Query: 808 EVGIETVIAEAKPQQKAEEVKNLQ 827
           EVGI TV AE  P  KAE++K+LQ
Sbjct: 818 EVGIGTVFAEIDPVGKAEKIKDLQ 837

BLAST of Sed0020675.2 vs. ExPASy Swiss-Prot
Match: A0A0P0X004 (Cation-transporting ATPase HMA5 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA9 PE=2 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 2.0e-187
Identity = 366/836 (43.78%), Postives = 530/836 (63.40%), Query Frame = 0

Query: 12  AATNEENAKHVEDGEAKTVVWVSGMSCSACAVSVENSIKHLPGVINAAVDFLNDRAQISY 71
           AA  EE   HV          V+GM+CSAC  +VE ++    GV   AV  L +RA + +
Sbjct: 45  AAAGEEEEAHVR---------VTGMTCSACTSAVEGAVSARRGVRRVAVSLLQNRAHVVF 104

Query: 72  LPNLIDEESLLKAIENAGFQATISKDGTNHRSRE----VCRIRVNGMGCKSCSSMVESVL 131
            P L+  E +++AIE+AGF A I  D    + +       + R+ GM C +C + VE +L
Sbjct: 105 DPALLKVEDIIEAIEDAGFDAEIIPDTAISQPKAQKTLSAQFRIGGMTCANCVNSVEGIL 164

Query: 132 EEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIGFDALIITIGEHISKIELKID 191
           + + GV+ A +AL     EV YDP V+N ++ + AI+D GF+A  +   E   KI L + 
Sbjct: 165 KRLSGVKGAVVALATSLGEVEYDPSVINKDEIVEAIEDAGFEAAFLQSSEQ-DKILLGLT 224

Query: 192 GMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPDITGPRTFIEVIESIKSEHFK 251
           G+H+E     + + L+ + G+   D++  +S+V I + P+  G R+ ++ IE+  +   K
Sbjct: 225 GLHTERDVNVLHDILKKMIGLRQFDVNATVSEVEIIFDPEAVGLRSIVDAIETGSNGRLK 284

Query: 252 ATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSMVFMYIPGIKQTLDIKVVNMM 311
           A +    A   +    E  +    L  S  LSIPVF   MV  +IP I+  L +      
Sbjct: 285 AHVQNPYARGASNDAHEAAKMLHLLRSSLFLSIPVFFIRMVCPHIPFIRSIL-MMHCGPF 344

Query: 312 NVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSA 371
           ++G +++W L + VQF++G RFY+ +Y+A+R GS NMDVL+ LGT A+Y YSV  +L  A
Sbjct: 345 HMGDLLKWILVSIVQFVVGKRFYIAAYRALRHGSTNMDVLVVLGTTASYVYSVCALLYGA 404

Query: 372 TSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIAKLKNLAPETATLLTLDDHGD 431
            +  F+   +FETS+M+ITF+L GKYLEVLAKGKTS+AI KL  L P TA LL  D  G 
Sbjct: 405 FTG-FHPPIYFETSAMIITFVLFGKYLEVLAKGKTSDAIKKLVELVPATALLLLKDKEGK 464

Query: 432 VIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVI 491
              E EI + L+Q  D++K++PG +V +DG+VVWG SHVNESMITGE+ P+ K     VI
Sbjct: 465 YTEEREIDALLVQPGDILKVLPGSKVPADGVVVWGTSHVNESMITGESAPIPKEVSSAVI 524

Query: 492 GGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSF 551
           GGT+N +GVLHI+   VGSE+ L+QI+ LVE++Q++KAPIQKFAD+++  FVP+VI LS 
Sbjct: 525 GGTMNLHGVLHIQANKVGSETVLSQIISLVETAQMSKAPIQKFADYVASIFVPIVITLSM 584

Query: 552 LTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGV 611
           +T++ WFL G +  YP SW+  + + F  +L F I+V+VIACPCALGLATPTAVMV TGV
Sbjct: 585 ITFLVWFLCGWVGAYPNSWISGTSNCFVFSLMFAIAVVVIACPCALGLATPTAVMVATGV 644

Query: 612 GASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVELMNNVVLEELLELTAATE 671
           GA+ GVL+KGG ALE A  V+ ++FDKTGTLT GK VV   ++ + + L + L L A+ E
Sbjct: 645 GANHGVLVKGGDALERAQNVNYVIFDKTGTLTQGKAVVTTAKVFSGMDLGDFLTLVASAE 704

Query: 672 VNSEHPVAKAIVEYA----------------KQLKEEQ-NPIWPEAQEFISIPGHGVEAT 731
            +SEHP+AKAIVEYA                +Q KE++ + +  + ++F ++PG GV+  
Sbjct: 705 ASSEHPLAKAIVEYAFHFHFFGKLPTSKDGIEQRKEDRLSQLLLQVEDFSALPGKGVQCL 764

Query: 732 VRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKP 791
           +  K+++VGN++L+  N + +P EAE+FL+D E  A+T +LV+ +    G++ +TDPLK 
Sbjct: 765 INGKRVLVGNRTLVTENGVNVPPEAENFLVDLELNAKTGILVSYDDDFVGLMGITDPLKR 824

Query: 792 GSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQ 827
            +  V+  LK M V  +M+TGDNW TA ++AKEVGIE V AE  P  KA+ V++LQ
Sbjct: 825 EAAVVVEGLKKMGVHPVMLTGDNWRTAKAVAKEVGIEDVRAEVMPAGKADVVRSLQ 868

BLAST of Sed0020675.2 vs. ExPASy Swiss-Prot
Match: Q9S7J8 (Copper-transporting ATPase RAN1 OS=Arabidopsis thaliana OX=3702 GN=RAN1 PE=1 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 2.3e-180
Identity = 352/817 (43.08%), Postives = 514/817 (62.91%), Query Frame = 0

Query: 25  GEAKTVVWVSGMSCSACAVSVENSIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKA 84
           G  K  V V+GM+C+AC+ SVE ++ ++ GV  A+V  L +RA + + PNL+ EE + +A
Sbjct: 54  GLRKIQVGVTGMTCAACSNSVEAALMNVNGVFKASVALLQNRADVVFDPNLVKEEDIKEA 113

Query: 85  IENAGFQATISKDGTNHRSREVCRIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEE 144
           IE+AGF+A I  +    ++  V +  + GM C +C + VE +L ++ GV++A +AL    
Sbjct: 114 IEDAGFEAEILAE-EQTQATLVGQFTIGGMTCAACVNSVEGILRDLPGVKRAVVALSTSL 173

Query: 145 AEVHYDPKVVNSNQFIVAIQDIGFDALIITIGEHISKIELKIDGMHSENSTIKVKESLES 204
            EV YDP V+N +  + AI+D GF+  ++   +   K+ L++DG+ +E     ++  L  
Sbjct: 174 GEVEYDPNVINKDDIVNAIEDAGFEGSLVQSNQQ-DKLVLRVDGILNELDAQVLEGILTR 233

Query: 205 VPGIEDIDIDMALSKVTISYWPDITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKE 264
           + G+    +D    ++ + + P++   R+ ++ IE      FK  +        ++   E
Sbjct: 234 LNGVRQFRLDRISGELEVVFDPEVVSSRSLVDGIEEDGFGKFKLRVMSPYERLSSKDTGE 293

Query: 265 IKQHYKYLLWSCALSIPVFLTSMVFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFI 324
               ++  + S  LSIP+F   ++  +I  +   L +       +G  ++W L + +QF+
Sbjct: 294 ASNMFRRFISSLVLSIPLFFIQVICPHI-ALFDALLVWRCGPFMMGDWLKWALVSVIQFV 353

Query: 325 IGSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSML 384
           IG RFYV +++A+R GS NMDVL+ LGT+A+YFYSV  +L  A + F++ T +F+ S+ML
Sbjct: 354 IGKRFYVAAWRALRNGSTNMDVLVALGTSASYFYSVGALLYGAVTGFWSPT-YFDASAML 413

Query: 385 ITFILLGKYLEVLAKGKTSEAIAKLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDV 444
           ITF+LLGKYLE LAKGKTS+A+ KL  L P TA LLT    G ++GE EI + LIQ  D 
Sbjct: 414 ITFVLLGKYLESLAKGKTSDAMKKLVQLTPATAILLTEGKGGKLVGEREIDALLIQPGDT 473

Query: 445 IKIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHV 504
           +K+ PG ++ +DG+VVWG S+VNESM+TGE+ PV+K     VIGGT+N +G LH+K T V
Sbjct: 474 LKVHPGAKIPADGVVVWGSSYVNESMVTGESVPVSKEVDSPVIGGTINMHGALHMKATKV 533

Query: 505 GSESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPK 564
           GS++ L+QI+ LVE++Q++KAPIQKFAD+++  FVP+VI L+  T + W + G +  YP 
Sbjct: 534 GSDAVLSQIISLVETAQMSKAPIQKFADYVASIFVPVVITLALFTLVGWSIGGAVGAYPD 593

Query: 565 SWLPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFA 624
            WLP +   F  +L F ISV+VIACPCALGLATPTAVMV TGVGA+ GVLIKGG ALE A
Sbjct: 594 EWLPENGTHFVFSLMFSISVVVIACPCALGLATPTAVMVATGVGATNGVLIKGGDALEKA 653

Query: 625 HKVSCIVFDKTGTLTIGKPVVVNVELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQ 684
           HKV  ++FDKTGTLT GK  V   ++ + +   E L L A+ E +SEHP+AKAIV YA+ 
Sbjct: 654 HKVKYVIFDKTGTLTQGKATVTTTKVFSEMDRGEFLTLVASAEASSEHPLAKAIVAYARH 713

Query: 685 L--------------KEEQNPIW-PEAQEFISIPGHGVEATVRNKKIIVGNKSLMMNNDI 744
                          K+ QN  W  +  +F ++PG G++  V  K I+VGN+ LM  N I
Sbjct: 714 FHFFDESTEDGETNNKDLQNSGWLLDTSDFSALPGKGIQCLVNEKMILVGNRKLMSENAI 773

Query: 745 EIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSKEVISILKSMEVKSIMV 804
            IP   E F+ D E   +T V+VA N  + GV+ + DPLK  +  V+  L  M V+ IMV
Sbjct: 774 NIPDHVEKFVEDLEESGKTGVIVAYNGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMV 833

Query: 805 TGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQ 827
           TGDNW TA ++AKEVGIE V AE  P  KA+ +++LQ
Sbjct: 834 TGDNWRTARAVAKEVGIEDVRAEVMPAGKADVIRSLQ 866

BLAST of Sed0020675.2 vs. ExPASy TrEMBL
Match: A0A6J1H484 (probable copper-transporting ATPase HMA5 OS=Cucurbita moschata OX=3662 GN=LOC111459458 PE=3 SV=1)

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 737/840 (87.74%), Postives = 783/840 (93.21%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEENAKHVED-------------GEAKTVVWVSGMSCSACAVSVEN 60
           M+KLPRRKRSPAATN+ENA  V +              EAK V+ VSGM+CSACAVSVEN
Sbjct: 1   MLKLPRRKRSPAATNQENANDVANIDNDETAAGVRKAAEAKVVLCVSGMTCSACAVSVEN 60

Query: 61  SIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVC 120
           SIKHLPG+++AA+DFLNDRAQI YLPNLIDE+S++KAIENAGFQATISKD  +HRSREVC
Sbjct: 61  SIKHLPGILDAAIDFLNDRAQIRYLPNLIDEDSIVKAIENAGFQATISKDSADHRSREVC 120

Query: 121 RIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIG 180
           RIRVNGMGC SCSSMVESVLEEMYGVQK HIAL N EAEVHYDPKVVNS QFIVAIQDIG
Sbjct: 121 RIRVNGMGCNSCSSMVESVLEEMYGVQKTHIALFNGEAEVHYDPKVVNSTQFIVAIQDIG 180

Query: 181 FDALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPD 240
           FDAL+ITIGEHISKIELKIDGMH+ENS+ KVKESLESV GIED+DIDM L+KVTISY PD
Sbjct: 181 FDALLITIGEHISKIELKIDGMHNENSSTKVKESLESVVGIEDVDIDMGLTKVTISYKPD 240

Query: 241 ITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSM 300
           ITGPRTFIEVIESIKSEHFKATIYP   GRE RKEKEIKQHYKY LWS ALS+PVFLTSM
Sbjct: 241 ITGPRTFIEVIESIKSEHFKATIYPANVGRENRKEKEIKQHYKYFLWSSALSVPVFLTSM 300

Query: 301 VFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVL 360
           VFMYIPGIKQ LDIKVVNMMNVGH+I+WNLSTPVQFIIGSRFY+GSYKA+RRGSANMDVL
Sbjct: 301 VFMYIPGIKQILDIKVVNMMNVGHLIKWNLSTPVQFIIGSRFYIGSYKALRRGSANMDVL 360

Query: 361 ITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIA 420
           ITLGTNAAYFYSVYIVLR+ATSP F+GTDFFETSSMLITFILLGKYLE+LAKGKTSEAIA
Sbjct: 361 ITLGTNAAYFYSVYIVLRAATSPAFDGTDFFETSSMLITFILLGKYLEILAKGKTSEAIA 420

Query: 421 KLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVN 480
           KLK+LAPETATLLTLD HG+VI E EI+ ELIQKNDVIKI PG +VASDGLVVWGESHVN
Sbjct: 421 KLKHLAPETATLLTLDGHGNVISEVEISGELIQKNDVIKITPGAKVASDGLVVWGESHVN 480

Query: 481 ESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPI 540
           ESMITGEAKPVAKRTGDKVIGGT+NENGVLHIK THVGSESSLAQIV+LVESSQLAKAPI
Sbjct: 481 ESMITGEAKPVAKRTGDKVIGGTMNENGVLHIKATHVGSESSLAQIVQLVESSQLAKAPI 540

Query: 541 QKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600
           QK ADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMD FELALQFGISVMVI
Sbjct: 541 QKLADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDRFELALQFGISVMVI 600

Query: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVN 660
           ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHK SCIVFDKTGTLT+GKPVVVN
Sbjct: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKASCIVFDKTGTLTVGKPVVVN 660

Query: 661 VELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGV 720
           V+LM+ +VLEELLELTAATEVNSEHP+AKAIVEYAKQ KE+ NPIWPEAQEFISIPGHGV
Sbjct: 661 VKLMDTIVLEELLELTAATEVNSEHPIAKAIVEYAKQFKED-NPIWPEAQEFISIPGHGV 720

Query: 721 EATVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDP 780
           EA VRNKK++VGNKSLMMNN IEI GE ESFL+DAEGMA+TAVLVA++RTVSGVI V+DP
Sbjct: 721 EAIVRNKKVVVGNKSLMMNNGIEILGETESFLVDAEGMAKTAVLVAVDRTVSGVIVVSDP 780

Query: 781 LKPGSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           LKPG+KEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT
Sbjct: 781 LKPGAKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 839

BLAST of Sed0020675.2 vs. ExPASy TrEMBL
Match: A0A1S3ATK1 (probable copper-transporting ATPase HMA5 OS=Cucumis melo OX=3656 GN=LOC103482737 PE=3 SV=1)

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 735/834 (88.13%), Postives = 781/834 (93.65%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEE---NAKHVEDGE----AKTVVWVSGMSCSACAVSVENSIKHLP 60
           M+KLPRRKRSPAA  EE   NA  + D E    AK VV VSGMSCSACAVSVENSIKHLP
Sbjct: 1   MLKLPRRKRSPAAATEEITKNATAINDDEATVAAKAVVRVSGMSCSACAVSVENSIKHLP 60

Query: 61  GVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVCRIRVNG 120
           G+++AAVDFLNDRAQI YLPNL D E++LKAIENAGFQATISKDGT+HRSREVCRIRVNG
Sbjct: 61  GILDAAVDFLNDRAQIHYLPNLTDAETILKAIENAGFQATISKDGTDHRSREVCRIRVNG 120

Query: 121 MGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIGFDALII 180
           MGC SCSSMVESVLE MYGVQK HI LL EEAEVHYDPKVVN NQFI+AI+DIGF+AL I
Sbjct: 121 MGCNSCSSMVESVLEAMYGVQKTHIVLLKEEAEVHYDPKVVNCNQFIIAIEDIGFEALPI 180

Query: 181 TIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPDITGPRT 240
           TIGE+I+KI+LKIDGMH+ENST KVKESL+ +PGI+D+++D  LSKVTISY PDI GPRT
Sbjct: 181 TIGEYITKIDLKIDGMHNENSTAKVKESLKLIPGIDDVNVDTTLSKVTISYRPDIIGPRT 240

Query: 241 FIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSMVFMYIP 300
           FIE++ESIKSEHFKATIYPE+  RETRKEKEIKQHYKYL+WS ALSIPVFLTSMVFMYIP
Sbjct: 241 FIEILESIKSEHFKATIYPEDTERETRKEKEIKQHYKYLIWSSALSIPVFLTSMVFMYIP 300

Query: 301 GIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVLITLGTN 360
           GIKQTLDIKVVNMMNVGHIIRWNLSTPVQF++GSRFY GSYKA+RRGSANMDVL+TLGTN
Sbjct: 301 GIKQTLDIKVVNMMNVGHIIRWNLSTPVQFVVGSRFYFGSYKALRRGSANMDVLVTLGTN 360

Query: 361 AAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIAKLKNLA 420
           AAYFYSVY+VLRSATSP FNGTDFFETSSMLITFILLGKYLEVLAKGKTS+AIAKLK+LA
Sbjct: 361 AAYFYSVYVVLRSATSPTFNGTDFFETSSMLITFILLGKYLEVLAKGKTSDAIAKLKHLA 420

Query: 421 PETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVNESMITG 480
           PETAT+LTLD HG+VI EAEI+SELIQKNDVIKI PG RVASDGLVVWGESHVNESMITG
Sbjct: 421 PETATILTLDRHGNVINEAEISSELIQKNDVIKITPGARVASDGLVVWGESHVNESMITG 480

Query: 481 EAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPIQKFADH 540
           EAKPV KRTGDKVIGGTVNENGVLHIK THVGSESSLAQIVRLVESSQLAKAPIQKFADH
Sbjct: 481 EAKPVTKRTGDKVIGGTVNENGVLHIKATHVGSESSLAQIVRLVESSQLAKAPIQKFADH 540

Query: 541 ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL 600
           ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL
Sbjct: 541 ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL 600

Query: 601 GLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVELMNN 660
           GLATPTA+MVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNV+L N 
Sbjct: 601 GLATPTAMMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVKLTNT 660

Query: 661 VVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGVEATVRN 720
            VLEELLELTAATEVNSEHPVAKAIVEYAKQ K+EQNPIWPEAQEFISIPGHGVEA V+N
Sbjct: 661 TVLEELLELTAATEVNSEHPVAKAIVEYAKQFKKEQNPIWPEAQEFISIPGHGVEAIVKN 720

Query: 721 KKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSK 780
           KKI VGNKSLMMNNDIEIP EAE FL+DAEGMAQTAVLVAI+R VSGVI V+DPLKPG+K
Sbjct: 721 KKITVGNKSLMMNNDIEIPREAEMFLVDAEGMAQTAVLVAIDRMVSGVIAVSDPLKPGTK 780

Query: 781 EVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           EVISILK+MEVKSIM+TGDNWGTANSIAKEVGIET+IAEAKPQQKAEEVK LQT
Sbjct: 781 EVISILKAMEVKSIMITGDNWGTANSIAKEVGIETIIAEAKPQQKAEEVKKLQT 834

BLAST of Sed0020675.2 vs. ExPASy TrEMBL
Match: A0A6J1K8H5 (probable copper-transporting ATPase HMA5 OS=Cucurbita maxima OX=3661 GN=LOC111491097 PE=3 SV=1)

HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 735/840 (87.50%), Postives = 782/840 (93.10%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEENAKHV-------------EDGEAKTVVWVSGMSCSACAVSVEN 60
           M+KLPRRKRSPAATN+ENA +V             E  EAK V+ VSGM+CSACAVSVEN
Sbjct: 1   MLKLPRRKRSPAATNQENANNVATIDNDETAARTREVAEAKVVLCVSGMTCSACAVSVEN 60

Query: 61  SIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVC 120
           SIKHLPG+++ A+DFLNDRAQI YLPNLIDE+S++KAIENAGFQATISKD  +HRSREVC
Sbjct: 61  SIKHLPGILDVAIDFLNDRAQIRYLPNLIDEDSIVKAIENAGFQATISKDSADHRSREVC 120

Query: 121 RIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIG 180
           RIRVNGMGC SCSSMVESVLEEMYGVQK HIAL   EAEVHYDPKVVNS QFIVAIQDIG
Sbjct: 121 RIRVNGMGCNSCSSMVESVLEEMYGVQKTHIALFKGEAEVHYDPKVVNSTQFIVAIQDIG 180

Query: 181 FDALIITIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPD 240
           FDAL+ITIGEH+SKIELKIDGM +ENS+ KVKESLESV GIED+DIDM L+KVTISY PD
Sbjct: 181 FDALLITIGEHVSKIELKIDGMDNENSSTKVKESLESVVGIEDVDIDMGLTKVTISYKPD 240

Query: 241 ITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSM 300
           ITGPRTFIEVIESIKSEH KATIYPE+ GRE RKEKEIKQHYKY LWS ALS+PVFLTSM
Sbjct: 241 ITGPRTFIEVIESIKSEHLKATIYPEKVGRENRKEKEIKQHYKYFLWSSALSVPVFLTSM 300

Query: 301 VFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVL 360
           VFMYIPGIKQ LDIKVVNMMNVGH+I+WNLSTPVQFIIGSRFY+GSYKA+R GSANMDVL
Sbjct: 301 VFMYIPGIKQILDIKVVNMMNVGHLIKWNLSTPVQFIIGSRFYIGSYKALRCGSANMDVL 360

Query: 361 ITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIA 420
           ITLGTNAAYFYSVYIVLRSATSP F+GTDFFETSSMLITFILLGKYLE+LAKGKTSEAIA
Sbjct: 361 ITLGTNAAYFYSVYIVLRSATSPAFDGTDFFETSSMLITFILLGKYLEILAKGKTSEAIA 420

Query: 421 KLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVN 480
           KLK+LAPETATLLTLD HG+VI E EI+SELIQKNDVIKI PG +VASDG+VVWGESHVN
Sbjct: 421 KLKHLAPETATLLTLDGHGNVISEVEISSELIQKNDVIKITPGAKVASDGIVVWGESHVN 480

Query: 481 ESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPI 540
           ESMITGEAKPVAKRTGDKVIGGT+NENGVLHIK THVGSESSLAQIVRLVESSQLAKAPI
Sbjct: 481 ESMITGEAKPVAKRTGDKVIGGTMNENGVLHIKATHVGSESSLAQIVRLVESSQLAKAPI 540

Query: 541 QKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600
           QK AD+ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI
Sbjct: 541 QKLADYISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVI 600

Query: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVN 660
           ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCI FDKTGTLTIGKPVVVN
Sbjct: 601 ACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIAFDKTGTLTIGKPVVVN 660

Query: 661 VELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGV 720
           V+LM+ +VLEELLELTAATEVNSEHP+AKAIVEYAKQ KEE NPIWPEAQEFISIPGHGV
Sbjct: 661 VKLMDTIVLEELLELTAATEVNSEHPIAKAIVEYAKQFKEEHNPIWPEAQEFISIPGHGV 720

Query: 721 EATVRNKKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDP 780
           EA VRNKK++VGN+SLMMNN IEI GE ESFL+DAEGMA+T VLVA++RTVSGVI V+DP
Sbjct: 721 EAIVRNKKVVVGNESLMMNNGIEILGETESFLVDAEGMAKTTVLVAVDRTVSGVIVVSDP 780

Query: 781 LKPGSKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           LKPG+KEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT
Sbjct: 781 LKPGAKEVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 840

BLAST of Sed0020675.2 vs. ExPASy TrEMBL
Match: A0A076ML20 (Heavy metal ATPase 5A OS=Cucumis sativus OX=3659 GN=HMA5.1 PE=2 SV=1)

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 735/834 (88.13%), Postives = 782/834 (93.76%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEE---NAKHVEDGE----AKTVVWVSGMSCSACAVSVENSIKHLP 60
           M+KLPR  RS AA  EE   NA  + D E    AK VV VSGMSCSACAVSVENSIKHLP
Sbjct: 1   MLKLPRWNRSTAAATEEITKNATAINDDEATTAAKAVVCVSGMSCSACAVSVENSIKHLP 60

Query: 61  GVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVCRIRVNG 120
           G+++AAVDFLNDRAQI YLPNL D E++L+AIENAGFQATISKDGT+HRSREVCRIRVNG
Sbjct: 61  GILDAAVDFLNDRAQILYLPNLTDVETILQAIENAGFQATISKDGTDHRSREVCRIRVNG 120

Query: 121 MGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIGFDALII 180
           MGC SCSSMVESVLE MYGVQKAHIALLNEEAEVHYDPKVVN NQFI+AIQDIGF+AL I
Sbjct: 121 MGCNSCSSMVESVLEAMYGVQKAHIALLNEEAEVHYDPKVVNCNQFIIAIQDIGFEALPI 180

Query: 181 TIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPDITGPRT 240
           TIGEH++KI+LKIDGMH+ENST KVKESLE V GI+D++ID  LSKVTISY PDI GPRT
Sbjct: 181 TIGEHVTKIDLKIDGMHNENSTTKVKESLELVLGIDDVNIDTTLSKVTISYRPDIIGPRT 240

Query: 241 FIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSMVFMYIP 300
           FIE++ESIKSEHFK TIYPE+  RETRK+KEIKQHYKYL+WS ALSIPVFLTSMVFMYIP
Sbjct: 241 FIEILESIKSEHFKVTIYPEDTERETRKQKEIKQHYKYLIWSSALSIPVFLTSMVFMYIP 300

Query: 301 GIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVLITLGTN 360
           GIKQTLDIKVVNMMNVGHIIRWNLSTPVQF++GSRFY GSYKA+RRGSANMDVL+TLGTN
Sbjct: 301 GIKQTLDIKVVNMMNVGHIIRWNLSTPVQFVVGSRFYFGSYKALRRGSANMDVLVTLGTN 360

Query: 361 AAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIAKLKNLA 420
           AAYFYSVYIVLR+ATSP FNGTDFFETSSMLITFILLGKYLEVLAKGKTS+AIAKLK+LA
Sbjct: 361 AAYFYSVYIVLRAATSPTFNGTDFFETSSMLITFILLGKYLEVLAKGKTSDAIAKLKHLA 420

Query: 421 PETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVNESMITG 480
           PETATLLTLD HG+VI EAEI+SELIQKNDVIKI PG RVASDGLVVWGESHVNESMITG
Sbjct: 421 PETATLLTLDLHGNVINEAEISSELIQKNDVIKITPGARVASDGLVVWGESHVNESMITG 480

Query: 481 EAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPIQKFADH 540
           EAKPV KRTGDKVIGGTVNENGVLHIK THVGSESSL+QIVRLVESSQLAKAPIQKFADH
Sbjct: 481 EAKPVTKRTGDKVIGGTVNENGVLHIKATHVGSESSLSQIVRLVESSQLAKAPIQKFADH 540

Query: 541 ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL 600
           ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL
Sbjct: 541 ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL 600

Query: 601 GLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVELMNN 660
           GLATPTA+MVGTGVGASQGVLIKGG+ALEFAHKVSCIVFDKTGTLTIGKPVVVNV+LMN 
Sbjct: 601 GLATPTAMMVGTGVGASQGVLIKGGRALEFAHKVSCIVFDKTGTLTIGKPVVVNVKLMNT 660

Query: 661 VVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGVEATVRN 720
            VLEELLELTAATEVNSEHPVAKAIVEYAKQ K+EQNP+WPEAQEFISIPGHGVEA V+N
Sbjct: 661 TVLEELLELTAATEVNSEHPVAKAIVEYAKQFKKEQNPLWPEAQEFISIPGHGVEAIVKN 720

Query: 721 KKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSK 780
           KKIIVGNKSLMMNNDIEIP E E FL+DAEGMAQTAVLVAI+R VSGV+TV+DPLKPG+K
Sbjct: 721 KKIIVGNKSLMMNNDIEIPREVEMFLVDAEGMAQTAVLVAIDRMVSGVVTVSDPLKPGTK 780

Query: 781 EVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           EVISILK+MEVKSIM+TGDNWGTANSIAKEVGIET+IAEAKPQQKAEEVKNLQT
Sbjct: 781 EVISILKAMEVKSIMITGDNWGTANSIAKEVGIETIIAEAKPQQKAEEVKNLQT 834

BLAST of Sed0020675.2 vs. ExPASy TrEMBL
Match: A0A5A7TJ12 (Putative copper-transporting ATPase HMA5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002680 PE=3 SV=1)

HSP 1 Score: 1418.7 bits (3671), Expect = 0.0e+00
Identity = 736/834 (88.25%), Postives = 779/834 (93.41%), Query Frame = 0

Query: 1   MVKLPRRKRSPAATNEE---NAKHVEDGE----AKTVVWVSGMSCSACAVSVENSIKHLP 60
           M+KLPRRKRSPAA  EE   NA  + D E    AK VV VSGMSCSACAVSVENSIKHLP
Sbjct: 1   MLKLPRRKRSPAAATEEITKNATAINDDEATVAAKAVVCVSGMSCSACAVSVENSIKHLP 60

Query: 61  GVINAAVDFLNDRAQISYLPNLIDEESLLKAIENAGFQATISKDGTNHRSREVCRIRVNG 120
           G+++AAVDFLNDRAQI YLPNL D E++LKAIENAGFQATISKDGT+HRS EVCRIRVNG
Sbjct: 61  GILDAAVDFLNDRAQIHYLPNLTDVETILKAIENAGFQATISKDGTDHRSGEVCRIRVNG 120

Query: 121 MGCKSCSSMVESVLEEMYGVQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIGFDALII 180
           MGC SCSSMVESVLE MYGVQK HI LL EEAEVHYDPKVVN NQFI+AI+DIGF+AL I
Sbjct: 121 MGCNSCSSMVESVLEAMYGVQKTHIVLLKEEAEVHYDPKVVNCNQFIIAIEDIGFEALPI 180

Query: 181 TIGEHISKIELKIDGMHSENSTIKVKESLESVPGIEDIDIDMALSKVTISYWPDITGPRT 240
           TIGE+I+KI+LKIDGMH+ENST KVKESL+ +PGI+D++ID ALSKVTISY PDI GPRT
Sbjct: 181 TIGEYITKIDLKIDGMHNENSTAKVKESLKLIPGIDDVNIDTALSKVTISYRPDIIGPRT 240

Query: 241 FIEVIESIKSEHFKATIYPEEAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSMVFMYIP 300
           FIE++ESIKSEHFKATIYPE+  RE RKEKEIKQHYKYL+WS ALSIPVFLTSMVFMYIP
Sbjct: 241 FIEILESIKSEHFKATIYPEDTEREARKEKEIKQHYKYLIWSSALSIPVFLTSMVFMYIP 300

Query: 301 GIKQTLDIKVVNMMNVGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVLITLGTN 360
           GIKQTLDIKVVNMMNVGHIIRWNLSTPVQF++GSRFY GSYKA+RRGSANMDVL+TLGTN
Sbjct: 301 GIKQTLDIKVVNMMNVGHIIRWNLSTPVQFVVGSRFYFGSYKALRRGSANMDVLVTLGTN 360

Query: 361 AAYFYSVYIVLRSATSPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIAKLKNLA 420
           AAYFYSVYIV RSATSP FNGTDFFETSSMLITFILLGKYLEVLAKGKTS+AIAKLK+LA
Sbjct: 361 AAYFYSVYIVSRSATSPTFNGTDFFETSSMLITFILLGKYLEVLAKGKTSDAIAKLKHLA 420

Query: 421 PETATLLTLDDHGDVIGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVNESMITG 480
           PETATLLTLD HG+VI EAEI+SELIQKNDVIKI PG RVASDGLVVWGESHVNESMITG
Sbjct: 421 PETATLLTLDRHGNVINEAEISSELIQKNDVIKITPGARVASDGLVVWGESHVNESMITG 480

Query: 481 EAKPVAKRTGDKVIGGTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPIQKFADH 540
           EAKPV KRTGDKVIGGTVNENGVLHIK THVGSESSLAQIVRLVESSQLAKAPIQKFADH
Sbjct: 481 EAKPVTKRTGDKVIGGTVNENGVLHIKATHVGSESSLAQIVRLVESSQLAKAPIQKFADH 540

Query: 541 ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL 600
           ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL
Sbjct: 541 ISKYFVPLVILLSFLTWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCAL 600

Query: 601 GLATPTAVMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVELMNN 660
           GLATPTA+MVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNV+L N 
Sbjct: 601 GLATPTAMMVGTGVGASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVKLTNT 660

Query: 661 VVLEELLELTAATEVNSEHPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGVEATVRN 720
            VLEELLELTAATEVNSEHPVAKAIVEYAKQ K+EQNPIWPEAQEFISIPGHGVEA V+N
Sbjct: 661 TVLEELLELTAATEVNSEHPVAKAIVEYAKQFKKEQNPIWPEAQEFISIPGHGVEAIVKN 720

Query: 721 KKIIVGNKSLMMNNDIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSK 780
           KKI VGNKSLMMNNDIEIP EAE FL+DAEGMAQTAVLVAI+R VSGVI V+DPLKPG+K
Sbjct: 721 KKITVGNKSLMMNNDIEIPREAEMFLVDAEGMAQTAVLVAIDRMVSGVIAVSDPLKPGTK 780

Query: 781 EVISILKSMEVKSIMVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQT 828
           EVISILK+MEVKSIM+TGDNWGTANSIAKEVGIET+IAEAKPQQKAEEVK LQT
Sbjct: 781 EVISILKAMEVKSIMITGDNWGTANSIAKEVGIETIIAEAKPQQKAEEVKKLQT 834

BLAST of Sed0020675.2 vs. TAIR 10
Match: AT1G63440.1 (heavy metal atpase 5 )

HSP 1 Score: 1069.7 bits (2765), Expect = 1.2e-312
Identity = 544/819 (66.42%), Postives = 679/819 (82.91%), Query Frame = 0

Query: 13  ATNEENAKHVEDGEAKTVVWVSGMSCSACAVSVENSIKHLPGVINAAVDFLNDRAQISYL 72
           +++E  A  ++D  ++ V  V GM+CSACA SVE +IK LPG+ +A +D LN+RAQI + 
Sbjct: 37  SSSETAAFEIDDPISRAVFQVLGMTCSACAGSVEKAIKRLPGIHDAVIDALNNRAQILFY 96

Query: 73  PNLIDEESLLKAIENAGFQATISKDGTNHRSREVCRIRVNGMGCKSCSSMVESVLEEMYG 132
           PN +D E++ + IE+AGF+A++ ++  N RSR+VCRIR+NGM C SCSS +E VL+ + G
Sbjct: 97  PNSVDVETIRETIEDAGFEASLIENEANERSRQVCRIRINGMTCTSCSSTIERVLQSVNG 156

Query: 133 VQKAHIALLNEEAEVHYDPKVVNSNQFIVAIQDIGFDALIITIGEHISKIELKIDGMHSE 192
           VQ+AH+AL  EEAE+HYDP++ + ++ +  I++ GF+A++I+ GE +SKI+LKIDG  ++
Sbjct: 157 VQRAHVALAIEEAEIHYDPRLSSYDRLLEEIENAGFEAVLISTGEDVSKIDLKIDGELTD 216

Query: 193 NSTIKVKESLESVPGIEDIDIDMALSKVTISYWPDITGPRTFIEVIESI---KSEHFKAT 252
            S   ++ SLE++PG++ ++I     K+++ Y PD+TGPR FI+VIES     S H KAT
Sbjct: 217 ESMKVIERSLEALPGVQSVEISHGTDKISVLYKPDVTGPRNFIQVIESTVFGHSGHIKAT 276

Query: 253 IYPE-EAGRETRKEKEIKQHYKYLLWSCALSIPVFLTSMVFMYIPGIKQTLDIKVVNMMN 312
           I+ E   GRE++K+ EIKQ+YK  LWS   ++PVFLT+MVFMYIPGIK  L  KV+NM+ 
Sbjct: 277 IFSEGGVGRESQKQGEIKQYYKSFLWSLVFTVPVFLTAMVFMYIPGIKDLLMFKVINMLT 336

Query: 313 VGHIIRWNLSTPVQFIIGSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSAT 372
           VG IIR  L+TPVQF+IG RFY GSYKA+RRGSANMDVLI LGTNAAYFYS+Y VLR+AT
Sbjct: 337 VGEIIRCVLATPVQFVIGWRFYTGSYKALRRGSANMDVLIALGTNAAYFYSLYTVLRAAT 396

Query: 373 SPFFNGTDFFETSSMLITFILLGKYLEVLAKGKTSEAIAKLKNLAPETATLLTLDDHGDV 432
           SP F G DFFETS+MLI+FI+LGKYLEV+AKGKTS+AIAKL NLAP+TA LL+LD  G+V
Sbjct: 397 SPDFKGVDFFETSAMLISFIILGKYLEVMAKGKTSQAIAKLMNLAPDTAILLSLDKEGNV 456

Query: 433 IGEAEITSELIQKNDVIKIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVIG 492
            GE EI   LIQKNDVIKI+PG +VASDG V+WG+SHVNESMITGEA+PVAKR GD VIG
Sbjct: 457 TGEEEIDGRLIQKNDVIKIVPGAKVASDGYVIWGQSHVNESMITGEARPVAKRKGDTVIG 516

Query: 493 GTVNENGVLHIKVTHVGSESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSFL 552
           GT+NENGVLH+KVT VGSES+LAQIVRLVES+QLAKAP+QK AD ISK+FVPLVI LSF 
Sbjct: 517 GTLNENGVLHVKVTRVGSESALAQIVRLVESAQLAKAPVQKLADRISKFFVPLVIFLSFS 576

Query: 553 TWIAWFLAGKLHLYPKSWLPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVG 612
           TW+AWFLAGKLH YP+SW+PSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVG
Sbjct: 577 TWLAWFLAGKLHWYPESWIPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVG 636

Query: 613 ASQGVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVELMNNVVLEELLELTAATEV 672
           ASQGVLIKGGQALE AHKV+CIVFDKTGTLT+GKPVVV  +L+ N+VL E  EL AATEV
Sbjct: 637 ASQGVLIKGGQALERAHKVNCIVFDKTGTLTMGKPVVVKTKLLKNMVLREFYELVAATEV 696

Query: 673 NSEHPVAKAIVEYAKQLK-EEQNPIWPEAQEFISIPGHGVEATVRNKKIIVGNKSLMMNN 732
           NSEHP+AKAIVEYAK+ + +E+NP WPEA +F+SI G GV+ATV+ ++I+VGNK+LM ++
Sbjct: 697 NSEHPLAKAIVEYAKKFRDDEENPAWPEACDFVSITGKGVKATVKGREIMVGNKNLMNDH 756

Query: 733 DIEIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSKEVISILKSMEVKSI 792
            + IP +AE  L D+E MAQT +LV+IN  + GV++V+DPLKP ++E ISILKSM +KSI
Sbjct: 757 KVIIPDDAEELLADSEDMAQTGILVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSI 816

Query: 793 MVTGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQ 827
           MVTGDNWGTANSIA+EVGI++VIAEAKP+QKAE+VK LQ
Sbjct: 817 MVTGDNWGTANSIAREVGIDSVIAEAKPEQKAEKVKELQ 855

BLAST of Sed0020675.2 vs. TAIR 10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1) )

HSP 1 Score: 634.0 bits (1634), Expect = 1.7e-181
Identity = 352/817 (43.08%), Postives = 514/817 (62.91%), Query Frame = 0

Query: 25  GEAKTVVWVSGMSCSACAVSVENSIKHLPGVINAAVDFLNDRAQISYLPNLIDEESLLKA 84
           G  K  V V+GM+C+AC+ SVE ++ ++ GV  A+V  L +RA + + PNL+ EE + +A
Sbjct: 54  GLRKIQVGVTGMTCAACSNSVEAALMNVNGVFKASVALLQNRADVVFDPNLVKEEDIKEA 113

Query: 85  IENAGFQATISKDGTNHRSREVCRIRVNGMGCKSCSSMVESVLEEMYGVQKAHIALLNEE 144
           IE+AGF+A I  +    ++  V +  + GM C +C + VE +L ++ GV++A +AL    
Sbjct: 114 IEDAGFEAEILAE-EQTQATLVGQFTIGGMTCAACVNSVEGILRDLPGVKRAVVALSTSL 173

Query: 145 AEVHYDPKVVNSNQFIVAIQDIGFDALIITIGEHISKIELKIDGMHSENSTIKVKESLES 204
            EV YDP V+N +  + AI+D GF+  ++   +   K+ L++DG+ +E     ++  L  
Sbjct: 174 GEVEYDPNVINKDDIVNAIEDAGFEGSLVQSNQQ-DKLVLRVDGILNELDAQVLEGILTR 233

Query: 205 VPGIEDIDIDMALSKVTISYWPDITGPRTFIEVIESIKSEHFKATIYPEEAGRETRKEKE 264
           + G+    +D    ++ + + P++   R+ ++ IE      FK  +        ++   E
Sbjct: 234 LNGVRQFRLDRISGELEVVFDPEVVSSRSLVDGIEEDGFGKFKLRVMSPYERLSSKDTGE 293

Query: 265 IKQHYKYLLWSCALSIPVFLTSMVFMYIPGIKQTLDIKVVNMMNVGHIIRWNLSTPVQFI 324
               ++  + S  LSIP+F   ++  +I  +   L +       +G  ++W L + +QF+
Sbjct: 294 ASNMFRRFISSLVLSIPLFFIQVICPHI-ALFDALLVWRCGPFMMGDWLKWALVSVIQFV 353

Query: 325 IGSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSML 384
           IG RFYV +++A+R GS NMDVL+ LGT+A+YFYSV  +L  A + F++ T +F+ S+ML
Sbjct: 354 IGKRFYVAAWRALRNGSTNMDVLVALGTSASYFYSVGALLYGAVTGFWSPT-YFDASAML 413

Query: 385 ITFILLGKYLEVLAKGKTSEAIAKLKNLAPETATLLTLDDHGDVIGEAEITSELIQKNDV 444
           ITF+LLGKYLE LAKGKTS+A+ KL  L P TA LLT    G ++GE EI + LIQ  D 
Sbjct: 414 ITFVLLGKYLESLAKGKTSDAMKKLVQLTPATAILLTEGKGGKLVGEREIDALLIQPGDT 473

Query: 445 IKIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHV 504
           +K+ PG ++ +DG+VVWG S+VNESM+TGE+ PV+K     VIGGT+N +G LH+K T V
Sbjct: 474 LKVHPGAKIPADGVVVWGSSYVNESMVTGESVPVSKEVDSPVIGGTINMHGALHMKATKV 533

Query: 505 GSESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPK 564
           GS++ L+QI+ LVE++Q++KAPIQKFAD+++  FVP+VI L+  T + W + G +  YP 
Sbjct: 534 GSDAVLSQIISLVETAQMSKAPIQKFADYVASIFVPVVITLALFTLVGWSIGGAVGAYPD 593

Query: 565 SWLPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFA 624
            WLP +   F  +L F ISV+VIACPCALGLATPTAVMV TGVGA+ GVLIKGG ALE A
Sbjct: 594 EWLPENGTHFVFSLMFSISVVVIACPCALGLATPTAVMVATGVGATNGVLIKGGDALEKA 653

Query: 625 HKVSCIVFDKTGTLTIGKPVVVNVELMNNVVLEELLELTAATEVNSEHPVAKAIVEYAKQ 684
           HKV  ++FDKTGTLT GK  V   ++ + +   E L L A+ E +SEHP+AKAIV YA+ 
Sbjct: 654 HKVKYVIFDKTGTLTQGKATVTTTKVFSEMDRGEFLTLVASAEASSEHPLAKAIVAYARH 713

Query: 685 L--------------KEEQNPIW-PEAQEFISIPGHGVEATVRNKKIIVGNKSLMMNNDI 744
                          K+ QN  W  +  +F ++PG G++  V  K I+VGN+ LM  N I
Sbjct: 714 FHFFDESTEDGETNNKDLQNSGWLLDTSDFSALPGKGIQCLVNEKMILVGNRKLMSENAI 773

Query: 745 EIPGEAESFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSKEVISILKSMEVKSIMV 804
            IP   E F+ D E   +T V+VA N  + GV+ + DPLK  +  V+  L  M V+ IMV
Sbjct: 774 NIPDHVEKFVEDLEESGKTGVIVAYNGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMV 833

Query: 805 TGDNWGTANSIAKEVGIETVIAEAKPQQKAEEVKNLQ 827
           TGDNW TA ++AKEVGIE V AE  P  KA+ +++LQ
Sbjct: 834 TGDNWRTARAVAKEVGIEDVRAEVMPAGKADVIRSLQ 866

BLAST of Sed0020675.2 vs. TAIR 10
Match: AT4G33520.2 (P-type ATP-ase 1 )

HSP 1 Score: 266.5 bits (680), Expect = 6.9e-71
Identity = 190/512 (37.11%), Postives = 278/512 (54.30%), Query Frame = 0

Query: 326 GSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLI 385
           G +  +   K++ +GS NM+ L+ LG  +++  S      +A  P      FFE   MLI
Sbjct: 300 GRKLVLDGIKSLLKGSPNMNTLVGLGALSSFSVSSL----AAMIPKLGWKTFFEEPVMLI 359

Query: 386 TFILLGKYLEVLAKGKTSEAIAKLKNLAPETATLLTLDDHGDVIGE-AEITSELIQKNDV 445
            F+LLG+ LE  AK K +  +  L ++ P  A LL LD  GD+     E+    +   D+
Sbjct: 360 AFVLLGRNLEQRAKIKATSDMTGLLSVLPSKARLL-LD--GDLQNSTVEVPCNSLSVGDL 419

Query: 446 IKIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHV 505
           + I+PG RV +DG+V  G S ++ES  TGE  PV K +G +V  G++N NG L ++V   
Sbjct: 420 VVILPGDRVPADGVVKSGRSTIDESSFTGEPLPVTKESGSQVAAGSINLNGTLTVEVHRS 479

Query: 506 GSESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPK 565
           G E+++  I+RLVE +Q  +AP+Q+  D ++  F   V+ LS  T+  W L G  H+ P 
Sbjct: 480 GGETAVGDIIRLVEEAQSREAPVQQLVDKVAGRFTYGVMALSAATFTFWNLFG-AHVLPS 539

Query: 566 SWLPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFA 625
           +    S     LALQ   SV+V+ACPCALGLATPTA++VGT +GA +G+L++GG  LE  
Sbjct: 540 ALHNGS--PMSLALQLSCSVLVVACPCALGLATPTAMLVGTSLGARRGLLLRGGDILEKF 599

Query: 626 HKVSCIVFDKTGTLTIGKPVVVNVEL-------MNNVVLE-ELLELTAATEVNSEHPVAK 685
             V  +VFDKTGTLT G PVV  V +       +N+   E E+L L AA E N+ HPV K
Sbjct: 600 SLVDTVVFDKTGTLTKGHPVVTEVIIPENPRHNLNDTWSEVEVLMLAAAVESNTTHPVGK 659

Query: 686 AIVEYAKQLKEEQNPIWPEAQEFISIPGHGVEATVRNKKIIVGNKSLMMNNDIEIPGEAE 745
           AIV+ A+        +  E   F   PG G  A V NK++ VG    +  +     G + 
Sbjct: 660 AIVKAAR--ARNCQTMKAEDGTFTEEPGSGAVAIVNNKRVTVGTLEWVKRHG--ATGNSL 719

Query: 746 SFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSKEVISILKSMEVKSIMVTGDNWGT 805
             L + E   Q+ V + ++ T++ VI   D ++  + +V+  L    +   M++GD    
Sbjct: 720 LALEEHEINNQSVVYIGVDNTLAAVIRFEDKVREDAAQVVENLTRQGIDVYMLSGDKRNA 779

Query: 806 ANSIAKEVGI--ETVIAEAKPQQKAEEVKNLQ 827
           AN +A  VGI  E VIA  KP +K   +  LQ
Sbjct: 780 ANYVASVVGINHERVIAGVKPAEKKNFINELQ 797

BLAST of Sed0020675.2 vs. TAIR 10
Match: AT4G33520.3 (P-type ATP-ase 1 )

HSP 1 Score: 266.5 bits (680), Expect = 6.9e-71
Identity = 190/512 (37.11%), Postives = 278/512 (54.30%), Query Frame = 0

Query: 326 GSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSATSPFFNGTDFFETSSMLI 385
           G +  +   K++ +GS NM+ L+ LG  +++  S      +A  P      FFE   MLI
Sbjct: 300 GRKLVLDGIKSLLKGSPNMNTLVGLGALSSFSVSSL----AAMIPKLGWKTFFEEPVMLI 359

Query: 386 TFILLGKYLEVLAKGKTSEAIAKLKNLAPETATLLTLDDHGDVIGE-AEITSELIQKNDV 445
            F+LLG+ LE  AK K +  +  L ++ P  A LL LD  GD+     E+    +   D+
Sbjct: 360 AFVLLGRNLEQRAKIKATSDMTGLLSVLPSKARLL-LD--GDLQNSTVEVPCNSLSVGDL 419

Query: 446 IKIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVIGGTVNENGVLHIKVTHV 505
           + I+PG RV +DG+V  G S ++ES  TGE  PV K +G +V  G++N NG L ++V   
Sbjct: 420 VVILPGDRVPADGVVKSGRSTIDESSFTGEPLPVTKESGSQVAAGSINLNGTLTVEVHRS 479

Query: 506 GSESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSFLTWIAWFLAGKLHLYPK 565
           G E+++  I+RLVE +Q  +AP+Q+  D ++  F   V+ LS  T+  W L G  H+ P 
Sbjct: 480 GGETAVGDIIRLVEEAQSREAPVQQLVDKVAGRFTYGVMALSAATFTFWNLFG-AHVLPS 539

Query: 566 SWLPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALEFA 625
           +    S     LALQ   SV+V+ACPCALGLATPTA++VGT +GA +G+L++GG  LE  
Sbjct: 540 ALHNGS--PMSLALQLSCSVLVVACPCALGLATPTAMLVGTSLGARRGLLLRGGDILEKF 599

Query: 626 HKVSCIVFDKTGTLTIGKPVVVNVEL-------MNNVVLE-ELLELTAATEVNSEHPVAK 685
             V  +VFDKTGTLT G PVV  V +       +N+   E E+L L AA E N+ HPV K
Sbjct: 600 SLVDTVVFDKTGTLTKGHPVVTEVIIPENPRHNLNDTWSEVEVLMLAAAVESNTTHPVGK 659

Query: 686 AIVEYAKQLKEEQNPIWPEAQEFISIPGHGVEATVRNKKIIVGNKSLMMNNDIEIPGEAE 745
           AIV+ A+        +  E   F   PG G  A V NK++ VG    +  +     G + 
Sbjct: 660 AIVKAAR--ARNCQTMKAEDGTFTEEPGSGAVAIVNNKRVTVGTLEWVKRHG--ATGNSL 719

Query: 746 SFLIDAEGMAQTAVLVAINRTVSGVITVTDPLKPGSKEVISILKSMEVKSIMVTGDNWGT 805
             L + E   Q+ V + ++ T++ VI   D ++  + +V+  L    +   M++GD    
Sbjct: 720 LALEEHEINNQSVVYIGVDNTLAAVIRFEDKVREDAAQVVENLTRQGIDVYMLSGDKRNA 779

Query: 806 ANSIAKEVGI--ETVIAEAKPQQKAEEVKNLQ 827
           AN +A  VGI  E VIA  KP +K   +  LQ
Sbjct: 780 ANYVASVVGINHERVIAGVKPAEKKNFINELQ 797

BLAST of Sed0020675.2 vs. TAIR 10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 250.0 bits (637), Expect = 6.7e-66
Identity = 178/531 (33.52%), Postives = 286/531 (53.86%), Query Frame = 0

Query: 326 GSRFYVGSYKAVRRGSANMDVLITLGTNAAYFYSVYIVLRSATSPFFN-GTDFFETSSML 385
           G        KA  + S NM+ L+ LG+ AA+  S    L S  +P       FF+   ML
Sbjct: 232 GRELLFDGIKAFGKRSPNMNSLVGLGSMAAFSIS----LISLVNPELEWDASFFDEPVML 291

Query: 386 ITFILLGKYLEVLAKGKTSEAIAKLKNL-APETATLLTLDDHGDVIGEA--------EIT 445
           + F+LLG+ LE  AK + S  + +L +L + ++  ++T  D+   +            ++
Sbjct: 292 LGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICINVS 351

Query: 446 SELIQKNDVIKIIPGGRVASDGLVVWGESHVNESMITGEAKPVAKRTGDKVIGGTVNENG 505
            + I+  D + ++PG     DG V+ G S V+ESM+TGE+ PV K  G  V  GT+N +G
Sbjct: 352 VDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINWDG 411

Query: 506 VLHIKVTHVGSESSLAQIVRLVESSQLAKAPIQKFADHISKYFVPLVILLSFLTWIAWFL 565
            L IK +  GS S++++IVR+VE +Q   AP+Q+ AD I+  FV  ++ LS +T+  W+ 
Sbjct: 412 PLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFWYY 471

Query: 566 AGKLHLYPKSWLPS----SMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASQ 625
            G  H++P   L        D+  L+L+  + V+V++CPCALGLATPTA+++GT +GA +
Sbjct: 472 VGS-HIFPDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLGAKR 531

Query: 626 GVLIKGGQALEFAHKVSCIVFDKTGTLTIGKPVVVNVELMNNVVLEELLELTAATEVNSE 685
           G LI+GG  LE    + C+  DKTGTLT G+PVV  V  +     +E+L++ AA E  + 
Sbjct: 532 GYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYEEQEVLKMAAAVEKTAT 591

Query: 686 HPVAKAIVEYAKQLKEEQNPIWPEAQEFISIPGHGVEATVRNKKIIVG-----NKSLMMN 745
           HP+AKAIV  A+ L    N   PE +  ++ PG G  A +  + + VG     +   +  
Sbjct: 592 HPIAKAIVNEAESL----NLKTPETRGQLTEPGFGTLAEIDGRFVAVGSLEWVSDRFLKK 651

Query: 746 NDIEIPGEAESFL-------IDAEGMAQTAVLVA-INRTVSGVITVTDPLKPGSKEVISI 805
           ND     + ES L             ++T V V      + G I ++D L+  ++  ++ 
Sbjct: 652 NDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGREGEGIIGAIAISDCLRQDAEFTVAR 711

Query: 806 LKSMEVKSIMVTGDNWGTANSIAKEVGI--ETVIAEAKPQQKAEEVKNLQT 828
           L+   +K+++++GD  G   ++AK VGI  E+      P++K E + NLQ+
Sbjct: 712 LQEKGIKTVLLSGDREGAVATVAKNVGIKSESTNYSLSPEKKFEFISNLQS 752