Sed0015561.1 (mRNA) Chayote v1

Overview
NameSed0015561.1
TypemRNA
OrganismSechium edule (Chayote v1)
Descriptioncucumisin-like
LocationLG05: 3289585 .. 3301482 (+)
Sequence length2256
RNA-Seq ExpressionSed0015561.1
SyntenySed0015561.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTATCCAACTTTGATGGGTGCAAAAATGAAGATTGATATTCATAAGAATGATATCATGGTTAAAGTGAAAAAATCGAAGGCAAGTGATGTTGGTATGCTACTTTTAATGGAGGCTGTTAAACAATCCCGTTGTAAACAATGAATACCTTAAATTGGAATGCACGGTTTTGGGGATACCGCACGTATTAAGTTATGATAAATGTTTCGTTAAATTGCATAATCTTCAAGTTAATCTATACTTCTATTATCTAGAATGGTATCAGTGATATTCTTTTGATTTTTGTTAGTTCTGTTCTTTATTTTGCCTGCTTTGCTGAAGAGATGATTGATAAAGTAATGCTATTTTAGAGATTAACATGTGCTACTTTCGAAGGCGTCTCCATTTCTCTACGGTCATGGAGTTCGCTTCTCCTTTAATAATTATATGTATGCTAATAAGTATTTCATCAGTAAATTTAACCTTCAAAATTTATCAAATTAAATTATTGTAGTGTTGAAGACTGTTTGGTGGTTCAATATGTTGGGATCTTTTAACTCCCATGTATAACAAGGTCAACTTATAAAGTCATGTTGATAAAAAAAAAAAAAAAACTATCTAATATGTCATGTCTAATTGTCTGAAAAAACAAAAATGTCATATCTAGTATTTCATATTTAACGTGTCAGATCCGTTTTTTTTTTGGAGGAGGCGGTCAACTTGGGTTGGCTGCCTTAAGTATTAGGAATTTTTGCATGTATATTAAAAAATAATAATAATAATTTCATGTATAGTACAATTTCTAAACTTATTCATTTGTAACATAAAAATGATTGAAGTCTTTCAGTTGAACTGATAGAAGTCTAAAGTCTATCGGTTGAATTGATAGAAGTCTATTAGTTGATTGATAGAAGTCTAAATTCTATCGATTGAACTGATAGAAATCTAAAATCCATCAGTTGAATTGATAGAAGTCTAAATAGGAATTTTGGGCAAAATGTACTATGAATGAAATTATTTTAAAAAATGGGCTACAATTATAATGAAGGGAAAAAAATATGCTATGGAAGATAAATTCCCAAAGTATTATTATAAGGGGATTTTGCATGTATATTAAAAAAAACAAAATAATTCCATGTATAGCACAATTTCCAAACTTATTCATTTATGGCACAAAAATGATTAAAGTCTATCAATTTGCATTGTTCAATCACTCAAAATCGATTGAAGTCTATCAGTTGAACTGATAGAAGTCTAAAGTCTATCGGTTAAACTAATAGAAGTCTATTAGTTGAAACGATACTATCAATTGAACTGATAGAAGTCTAAAGTCTATCAGATGAATTGATAGAAGTCTGATGAGGTTAGAATAACAAGTTTGGACAAAATGTGCTATGGATGAAATTATTTTTAAAAAATGTGTTATGGATGAAGTTATTTTTAAAAATGCACTATAATTATAATGAATAGTAAAAAATATGCTATGGAGGATAAATTTCCTAAGTATTACGATAAAATACAAAATAAAAATTACATAGCCGCCACAGAAGAAGTCACTCTTCCATATTTTCAAGCCATCGCTTATTATTATTTTAATTAAATCATTGATTTAGTTTATTAGGGACTGTTTGTTTGGCATATTTAAGAAACTGTTATGACTGTTTGTTTGGCATATTTAAGGAGCTGTTATGAATGGGCTGTTATGAATGGTCTGTTTAGATGATGGTTTTTAGCAGCCCATATAACACCCCAAACCAAAGACAAGTTGAAGCGGGTGTACTACTAAAAAACGAGCATTTATTACTTTGTTTGAGCGGTTAACCTTTATATATATAATAGTTCCTCTTTAGAAAGAATATTTTTTTTTCCTTGAAGCTGCACCAAGGGGTGTAGATAGGTTAGGTTGAATCGAAAAGAATTTTTGGACAAACTCAATTTTTCGGGTTGATGAATCTATTAACCTGCCTAATGCAACTCAAGCTATATATTTTCAAATTAAGTTGGTTGTTATTATTTTTTTAATTTGAATATATTTTTGTCTATTTACTCTAAATTTTAAAATAAAACAACTTCATAACTTCTAAATTTTACTTATCTGGAATATTATGTTCGAGTTAAGTTGGGTGTTATTATGAATATTATCTTCTAGAAATTAGAACTAATAATAGAATGAATTTCAAAAGATAAAAAAGTATAAAGATAATAATAAAATAATTTTTTTGAGTACATATTGGGAGAGAGGGAATATCCACCAAACATTGAAATCACTGCGTCACTAACCCATTCAGTTAAAACAATATTTTTTTATACTAGATAATAAATTCACGTTACTCTACTAAAAAGATTAATATATATATATATGTATAGAAATCAAAAAAATAAAAATGAGGGAGACCACCCTCTACAAAAGATTTTAAACTCACCCACAACCCGAGCTACGACCGAACCAAAAAAAAAAAAAAAGTAAAGAGAAAATACATTCTAACAAAGAGGAAAGTCCTTCGAACAACAATCTTACCAAACAAGAAGCACACAACTTCAAGAAAATAGATCTGATAGCTAACTTCAACACTTTTAATATTGAATTTTTAAAAGTTTTGTTGGATAATTCAAATAAAATAGGTTCCACCGAGAAATATGACCCCAATATAGTTGGAAAGAACATCAAGTCCAAAGAGGAACCTTACCTTTTCCACGAGAAAAATCTGTAAAACCTATTATAAGAATAACATTTGTCCGTTAGCTCTTTTTCTGAACATGCTAACAACACCATCAACCAAAACGACGCCAACTTACTCCATAACCCAACCAATAACCAAAATAAACTTATAGACTAACAAACCAAAATCACAAACCATGAAAACGAGTAACATAAGACTCTTTCCTAAAAATCCACATTTAAATAATTTATTTTTTGCAACATATTATAAATAAACATCTAAACGAACTATTATTATAACTTTAATTTTAATCACTTAAAATAATTACTCATTCTAATCGGTCAAAATAACCGCTCGCTTATGCAATTATTCATCTAAATACCTTATCACTTTTAACACCAAGTTGAGAGGTCACTTCCATATGTTTTAATCAATACAATAGCTTACTTTTGTTGACATTTCAAAAGTGTATATATTTTCCACAGAAATTCCAATCACATGATATATAAATAGTACCATTGCATTTATCATTTCAATAACTTATGTTCAATACCTCTTCCTTTTCAGTTTCAAACTGCAAAAAAAATTCTTCCTTTCATTCAATGTTGTCTCTTTCAAAGCTTCTTTTCCTCAGCTTCTGTTTTTCTCTCCTCGTTTCAAGCTTGAGTTTTGAAGATGAAAATCGAAAGGCAATTCTTTAATTAATAAAACTCGTTGGATAACTATTTAATTTTTAGATTTTTTTTTTAGTTAAGCTTAATTTTATTCATTATCTTTTTTTTTTCTTTTCTAATATGTAATTTTTCATTGTGCAGACATACATCGTATACATGGGGAGTCATCCCAAAGACAAAATTTCAACTTCATCTCATCACGTGAGAATGCTACAAGAAACTATTGGCAGGTAATTATTATTACGTTGAAAAGTATATTGACGAGTAGGGACTGCCAGTGGTTGGTTCCACTCGATATGAGTATCGGATTGTCCTAAGATTTTCCATCGAAAAAGTGATGAGTATTCTAATTCACGTCTAATTTGGAATGTTTAAGATGATAAGTTTAATCTACGTGGTCATTTACTTAAAAATTAATTTCCTACGAGTTTTCTTGACATCTAATTGTTACAGAGCCACACAGTATATCATGTGATATTAGTTGAGGTGCATGCAAATTGACTCGAACAGTCATACATATAAAAAAAAAAAAAAACTTGCTAAAGATTAGTTACTTCAAAAAAAAAAAAAAACAGAATTATTATAGTTCTCTCATTATGAAATATCATGTTAGAGCCTTTTAAATAGATATTCTAATAAAAATGGATCCGACCCAAAATTTATAAATATAAAAAAGTATCGATCTTGATGGGGGTATAATAAGAATCTCGTCACCGCTTGATCATGAATCCAAACAAGGGTGCTTGGCTAAGAACCCAAACCATCAACTCTAATAGATTCCAACTTGAATCGGTATCGGTTTCGGTCGGTTTGGACAACTTTTTGTCTTTTGATCTTTTTTGGGTTTTTCCTTGCATCCCTATTGACCTAAAAGTAATTAAACCCTTTTGTTTCAGCACTTTTTCTCCACAAAGTTTATTACATAGCTACAAGAGAAGTTTTAATGGGTTTGTGGTGAAGTTGAGTGAAGCCGAAGCACAAAAAGTTTCAGGTACATTATCATCATTCATAAACAATTTTGTTAAATAATATAGAGTTTTACCTCAAAACTAATTGACAATTACGAGAGTAACTCATATCAGTTTGGACTTACCAATCTTAAATTAGATCTCAAATTTTATTTTCATTGGATTAAATATCAGTTTGGACTTATGGGCTCTGATACCATATTAGATAATATAAGATTCCAACTTAAAACAATGAAGAGAATAATTTATATATCTCTTATATATTGTCATGAGCTCTCCACCCTATCGTGATATATCTTTAACAACATAAACTTGGTAATTTCAGAAATGGAAGGTGTGATATCTGTTTTTCAAAATGAAAGAAAAGAATTGCACACAACAAGATCATGGGACTTCATGGGTCTGTCTCAACAAGCTAGTCGAGTTCCATCTATAGAAAGTGATATCATTGTTGGAGTATTTGACTCAGGCATTTGGCCCGAATCACCTAGCTTTGACGACAAAGGATATAGCCCACCACCATCTAAGTGGAAGGGTACTTGTGAAGTCTCGGCCAATTTCTCTTGTAACAAGTAAGTTACTTTTTTTTAAATATTTATACTATGCGAACTTATGTGCAATTTTGAGTATTAAGTAAATTTGTATAAAATTAATTTTTAGATTGAGTGATTACTCTAGGTTAAGTAGTTTTAAATATTATATATACTTTTTTTTTACTTATTGAATGTAAGTCACTCTTATTGTAACAACTAAGCTTAACTTTTAGAGGTAGTCATCTAGATAAACGGAATCTTTAGATATTTGGAATGATAGTTATTCAAGTAGTCATGTTTGTTTTGTAGGACGAAAATCCAAAAATATTTAGATAATTGTATTTTTTTTATAGGTTTCGTTAATGAAAATGTCTTAAATGCCTTTATAACTTATTCATATATTTTATACATTAATTTATATAACATATATTTTCTTTTTAATTTAATTATTATATTTTAAATAAATTTAATTATAAACAAAATTTATTGGTTAAACAAGTGATAAAATTAGTTAAATATAGAAAGTGTGGAAAGTAATATTTGAAATATATAATTTTAAAAAATAATTCTCTTTAAATTTTTATCATAAATAATCAAAATATTATTCAATTTAATAAAAACTATTTTATATCAAGAGGTAAAATATAAATATAATTAATTATGTATGGTTAATTGTTAATGTATATATAATAATATACTAAAAAAAAGAATATATTATTTTTGTTAAAGCTACAAACTACTTACAAATTATGAAAAAAAAATTGATAAAAATATAAAACAATCATGAAGGGTAAAATGTGTCATTTGAGGATGCACTATATATGAATATTCTAAAAATCCATGTTGAAGGAGGACATCCAAATAGTTCTTAAATAGGAAGTTCGTGCATCCTAGTTATGAGAGTTGTTAATGTAGTAGTACATGGAAGTAAGCATGTTGTAGAATAATGTGCAAGCGCCATGACTCCCATTGCCAATTAGATTCACCAAGGCACTTATGATGACAAACTAATGAAGTCTTTAGCATACTATGCGCGCCTTATGAACTTCATGCGAAAACATTATGGATTTTCTTATCGACCAAGTGGGAGACTGTTGGAAAATATCAAATGTTTCATTCTTCATTGAGAATGAAACTTGTATTCTCCATTAATATGGCCTATTTAGAAAATTACTTATGTTTTCCAATTGGTATATTAAGAAATGTTTGTGAGAGGTGAGTTATGTTTCTCTCAAGTTTATGAGAAATGTTCATGGAATGTTAGTTTCACTTCTCTAAAATTTATGAGAATTGAGTTTCACTTCATTTAAGTTTATGTTTCTTTTAAGTTAACTTTGATGGAAAGTTAAACTTATGTTTAAATGAAGACATACATTGGTCATCTTTCTACCTATAAATAGGAACACATTTGTCCATCTCAAATGTGTGAAAGTGAAAGAGAAAGAAACATTAAGAAAGCTTGAAAAACACATTCAAGCTTATAGGCAAGGAGGGAGAAACCAATTTCTTCTATTGAAGCAATTGTGAGAAAGCTTCAACATCAACCCTCATTCCTTGTGTTGAATCAAAAGGTTCTTGAATCACAATGTCATGAGGTATATTATAATCCCAATAAGATTCAATTACATTCCGTAATAATCATGTTATTCAAGATTTTAGGATTCATGTTATGCATAAAATATCTAATACAAACATCTTGAAATGCTTGAATATCGTCTCACATTCCTGCAAAATAATTTAGATGTCCATTTTTTGGGAATCTCAAATTGCCTCAAATGAGCCCCCTTGTAGTTTTAAAGTTTCTTTGATTTTAATTTTATTATTATTATTAAATGTTTATTTTTTGGGGTTTTTGCATGTATATTAAAAAAAACAAAATAATTTCATGTATAGCACAATTCCCAAACTTATTCATTTGTAACACAAAAGTGATTGAAGTCTATCACTTTGCCGATTGAAGTCTATCAATTGAATTGATAGAAGTCTAAAGTCTATCAGTTGAACTGATAAAAGCCTATTAGTTGACTGATAGAAGTCTATCAATTGAACTGATAGAAGTCTAAAGTCTATCGGTTGAACTGATAGAAATCTAAAATCTATCAGTTGAATTGATAGAAGTCTAAATATGAATTTTGGGCAAAATGTACTATGAATGAAATTATTTTTTAAAAATGGGCTACAATTGTAATGAAGAGCAAAAAATATGCTATAGAGGATAAATTCCCAAAGTATTATTATAAGAGGATTTTGTATGTATATTAAAAAAAACAAAATAATTCCATGTATAGCACAATTCCTAAACTTATTCATTTGTGTCACAAAAATGATTGAAATCTATCAATTTGCATTGTTCAATCACTCAAAATCGATTGAAATCTATCAGTTGAACTAATAGAAGTTTAAAGTCTATCGGTTGAACCGATAGAAGTCTATCAATTGAACTGATAGAAATCTAAAGTCTATAAATTGAAATGATAGACGTCTGATGATGTTAGAGTAGTAATTTTGGACAAAATGTGCTATGAATGAAATTATTTAAAAAAATGTTTTATGGATGAAATTATTTTAAAAAATGTGCTATAAAGAGCCAAAAAAAAAAATGCTAGGGAGGATAGATTCCCTTATTTTTTTTATCTTAAAAAATCGATTGCCACTATAAATATTTTCTCAATAAATTTTATAAATAGGTTCTATCATTTTGCATTAAAAGTATTAGTTTTTATAGAAATAATTTTGATTAAAACTATCGGTAGTATAGATACCAAATTTATATTTATATTCAAAATGCATAGATAAACTATTATATGTTGATTCAAGGGTCAAACGTTTTATAAGAAATAAAAATTCATTTCTTTTGAAAAAGCCTAAAAAACATAAATTAGGATTCTATTAAAAAAATGTTGAATAAAAAAAGCACCATCAAAATAAGAAATAATGAGGAACCACAAGAAGACTTCAAATTATAACATAGATATTTATTTTTTGATTGTCAATTGATTTTAAAATACAACCTATATTACCTTATACAAGTAGATGCCTAATATAATATTGGATGTTGAATATATAGTAAAATTATTGGAGCTCGATCATATCGCATCAATGGAATGTATCCAAAAACAGATATCCAAAGTCCAAGAGATTCACATGGCCATGGTACACACACAGCATCCACAGTGGCAGGCGGGTTGGTTGAGCGAGCGAGCATGCTCGGTCTCGGCATGGGAACAGCAAGGGGAGGAGTCCCATCGGCTCGAATTGCTGCCTACAAAATATGTTGGTCCGATGGCTGCTCTGATGCCGACATTCTTGCTGCATTCGACGATGCCATTGCCGATGGGGTCGACATCATCTCTTTCTCGGTTGGTGGAAACAGCTCGAAAGATTACTTTAAAGACTCCATGGCCATTGGAGCTTTCCATGCAATGAAGAAAGGAATCCTGACGTCAATGTCTGCTGGGAACAGCGGCCCAGGCCCTTCCACAATTAGAAACTTCTCGCCATGGTCTTTGTCTGTGGCGGCCAGTACTACAGATAGAAGATTTTTTACTCGAGTTCAACTTGGAGATGGAACAAGTTTCATGGTAAGTCACTCAATCATCTTTACATTTTATAATTATTAATTCAATCGGTTAGAGATCCTCATCTTATCACACATTAAAAATTTTAAGACATTTATAAAATATGTAAACTGCTAACTTTGTTATGAAAACGGCAATTAACAATGGTAAAATGAAGATATGAAAAAACAGATAATGGTAAACGTGAGAAAGACGAAGAAACATCACATAAATTTACATGTTTCACCAACGTTGTGTTAGCTAGTTCACGGGTAGAGGAGCAGAGCAATATTATTGATATATCTCTTTTCGAAAAAAAAAAGAGAGCAATATTATTGAGAGGCACCGAAATTTACAGAGATTATGTCATTATACAGTTAAGGTTTAATTCGAGTTTATATAGACACTCTAATTAAACTTTATTACAGAATGCTTAAGCATTAGCCGGAATGCGGACGTAGATGCGCGCTACAGAAATTAGAATGCTGATGCGCAAATACATGTAAAACACATTGCAGCAATGCACTGATGCGCAAATAATGCACTTCATAATTAATTTTGTGAAAGAATTGTTTGATATCTAATATCAATCTAGCGTTTCGTGTGGGTAATTTAGTTCACAAATTCAAATCTAATTCAGCTAGTAAACATAAGCATGTAGTAATTCAATCAATAAATTAAGATCCAACCTATGCAAATAATTTAAGTGATAAAAGTCAGATCTAATCTAAGATGGTGGCACCATTCGAGATCCCCATCTTTTCAAATTCAATTACAATAATAAAAATTGGCCTTTTTAAATCATAGAACATAACATTAAAATAAATCTTCTTTAATTTTTTCATTCCTACGTCATTCAATATATATATATATATATTTTGGATCTTTTTAAAATTAAAATTTGATGGAATTGATGCGTATATTTTCATTGTGTTATAATAGGGAGTCACCATTAATACATTTGATCTAAATGGAACACAATATCCATTGGTATATGCCGGAGATATTCCAAATATTACTGCTGGCTTTGATAAATCAATGTCCAGGTAACCCTCTCAATTATCCATGTCACTATTTCATTTTTTATGATTTTTGTATATATTAATCAAGTAACTACATATTTGATTGGTTATTCTTTTAAAATCGGTGAGTATTTGAGTTAGTTTATACGTAACTTATTATGCTTGATTTGGTGCCTTCTAATATTTAAAAACCTTATATTTTTTGTGAAATAGATTGTGCTTGGAAAATTCAGTGGACAGGGAGTTAGTGAGGGGTAAAATTGCCCTTTGTGATAAAATTTTATCACCAAAAAATGTGAGTTCTTTAAAAGGTGCAATTGGGATTGTAATGCAAGACAATCGTTCAAAAGATCGCACATCCTCTTTTCCATTGCCTGCCTCCCACCTTGACACACAAGGACGAGAAGCCATTTCTTCTTATCTCACCTTAACTAGGTAATTATTATATTGTTTTGATCCATCTATTTTTAACTTGTTTATAACCGAACAATCAAATCATTGCGCCACTCATCCATCCAGTATTATTATTATTTATTTTTTTTATTAAAAGTATCTAGAGACTAAAATTAAGTATTTGTAAGTTTAGGAATCAAAATAGAAAATAGTAGAACTTAAATGTCTGAAAATTATAAACGAAGATTTGAATTCCATACTATATTATAACCCATTAATTATAAATGCATTCTTGATATTTATTTAATCGTATGATCATAATTTTCAGCCTTCCAACAGCAACTATATTAAAAAGTACAGAAGGGAAAACATCTACTTTTGTTGCATCTTTCTCTTCAAGGGGTCCAAATCCAGTAACACTTGACATTCTTAAGGTCATTATAGTTCAATTTTATTTTATTTTAAGAATGGTAAATTTTATGATAGTTTTCTTATTAATTAATTTGTTTTTTTTGGTTTTTTCTTTTGTGGAATTGCAGCCCGATTTAACTGGTCCAGGAGTTGAAATTCTTGCAGCATGGTCTCCTATATCTCCACCTTCAGGAGCTGAAGATGATAATAGACATATTTTGTTTAATATCATTTCAGGAACTTCAATGTCTTGTCCACATGCTACCGCAGTTGCTGCATATGTTAAATCATTTCATCCTTCTTGGTCTCCTGCTGCACTTAAATCAGCTCTTATGACAACAGGTAATTAAACGATTAATTTTGCTGTCAAACACTTTTATATATGCTAATAATTTTGATATATGCAAATTATAGCCAAGGAAGCTTAACAAATTGCTAAAATACTATCTATACTAACGTCGGATGGAAAAAAAAATCTATACTAACATGAGTTTCTTTTCAACTGATTGAGTGTATAACTTTTTTTTTGGAAAGATTTTATATATATTAATCAGTTGAATCATTTTCACTCTTAGGATGAATGATTTATGATAAATAGGCACCATGATAAAATCAATGATGTTAAGTGAGAATGTCTAAACTGTTAAGAGCTGTAAACTTTTTATCCACTTTAAATCTTTCAGATTAGGAAGAAGTTTAAGCAAATTGGTTGTGTTATTATGTCAAGTCATAGAGATGTAGAAAAATGTCCGTATCTCATGTGCTTTATGAATTTCCATCGAAAAAGTGATGATACTTTATAATATTTATTAGATTTATAGATGTGAGCTGCTAATACACTTACGATCAAATTAATTTTGAGATGAAACATAACGTGCCTAATATGGTAATTAACATAGTTCATGAAGTTTAAAGTATTCGATCCAAAAAGTTATAATTCAAGAACGATAAATACAAAGAAGCATCATCTAAAAAAAAATTGAAATTGAGATCGGTCAATTTTTTTTTGGCATGAGTATGCATTTTTTTGTCCTATCACAACACCATGAAACTAAATCTAAATAAATTTATGCAGCATTTCCCATGCAAGCAGAGCTAAACAAAGATGCAGAATTTGCATATGGTTCAGGCCATATAAATCCATTAAGCGCAATAAACCCTGGATTAATCTACAATGCTACAGAAATAGACTATATAAAGTTTCTATGTGGTCAAGGTTATAGCACAAAACAACTCCAACAAGTGTCTGGAGATAGCAACACTTGCAATTCTGCAAACAAATTTGACACAGTTTTCAATCTAAACTACCCTTCATTTGCTCTTTCCACAACAATCTCAACCTCATTCAGCCAAGTTTACAAAAGAAGGGTCACAAATGTTGGGTCAGCAAACTCAACATACAAAGCTTCAATATTTGCTCCTTTGGGACTTAATATTATAGTGAATCCTTCTGTACTTTCATTCAAAGCTTTGGGAGAAGAGAAAAACTTTGAGGTTACAATTGAAGGAAGTATTGGTAGTAGCATTGCATCTGGTTCCTTGGTTTGGGATGATGGTCAACACAAAGTCAAAAGTCCTATAATTGTATTTGATGTTAAGACATTTATTAGTAATTAGTGTACTCTTTTGTAATTTTACTCTTGCCAAAAGTCAGTTTGGCCTAGCAATAATTGGCATGTACGTTTGATGGAGATGGGA

mRNA sequence

ATGACTTATCCAACTTTGATGGGTTTGGAAAGAACATCAAGTCCAAAGAGGAACCTTACCTTTTCCACGAGAAAAATCTGTAAAACCTATTATAAGAATAACATTTACATACATCGTATACATGGGGAGTCATCCCAAAGACAAAATTTCAACTTCATCTCATCACGTGAGAATGCTACAAGAAACTATTGGCAGTTGAGTGAAGCCGAAGCACAAAAAGTTTCAGAAATGGAAGGTGTGATATCTGTTTTTCAAAATGAAAGAAAAGAATTGCACACAACAAGATCATGGGACTTCATGGGTCTGTCTCAACAAGCTAGTCGAGTTCCATCTATAGAAAGTGATATCATTGTTGGAGTATTTGACTCAGGCATTTGGCCCGAATCACCTAGCTTTGACGACAAAGGATATAGCCCACCACCATCTAAGTGGAAGGGTACTTGTGAAGTCTCGGCCAATTTCTCTTGTAACAATAAAATTATTGGAGCTCGATCATATCGCATCAATGGAATGTATCCAAAAACAGATATCCAAAGTCCAAGAGATTCACATGGCCATGGTACACACACAGCATCCACAGTGGCAGGCGGGTTGGTTGAGCGAGCGAGCATGCTCGGTCTCGGCATGGGAACAGCAAGGGGAGGAGTCCCATCGGCTCGAATTGCTGCCTACAAAATATGTTGGTCCGATGGCTGCTCTGATGCCGACATTCTTGCTGCATTCGACGATGCCATTGCCGATGGGGTCGACATCATCTCTTTCTCGGTTGGTGGAAACAGCTCGAAAGATTACTTTAAAGACTCCATGGCCATTGGAGCTTTCCATGCAATGAAGAAAGGAATCCTGACGTCAATGTCTGCTGGGAACAGCGGCCCAGGCCCTTCCACAATTAGAAACTTCTCGCCATGGTCTTTGTCTGTGGCGGCCAGTACTACAGATAGAAGATTTTTTACTCGAGTTCAACTTGGAGATGGAACAAGTTTCATGGGAGTCACCATTAATACATTTGATCTAAATGGAACACAATATCCATTGGTATATGCCGGAGATATTCCAAATATTACTGCTGGCTTTGATAAATCAATGTCCAGATTGTGCTTGGAAAATTCAGTGGACAGGGAGTTAGTGAGGGGTAAAATTGCCCTTTGTGATAAAATTTTATCACCAAAAAATGTGAGTTCTTTAAAAGGTGCAATTGGGATTGTAATGCAAGACAATCGTTCAAAAGATCGCACATCCTCTTTTCCATTGCCTGCCTCCCACCTTGACACACAAGGACGAGAAGCCATTTCTTCTTATCTCACCTTAACTAGCCTTCCAACAGCAACTATATTAAAAAGTACAGAAGGGAAAACATCTACTTTTGTTGCATCTTTCTCTTCAAGGGGTCCAAATCCAGTAACACTTGACATTCTTAAGCCCGATTTAACTGGTCCAGGAGTTGAAATTCTTGCAGCATGGTCTCCTATATCTCCACCTTCAGGAGCTGAAGATGATAATAGACATATTTTGTTTAATATCATTTCAGGAACTTCAATGTCTTGTCCACATGCTACCGCAGTTGCTGCATATGTTAAATCATTTCATCCTTCTTGGTCTCCTGCTGCACTTAAATCAGCTCTTATGACAACAGCATTTCCCATGCAAGCAGAGCTAAACAAAGATGCAGAATTTGCATATGGTTCAGGCCATATAAATCCATTAAGCGCAATAAACCCTGGATTAATCTACAATGCTACAGAAATAGACTATATAAAGTTTCTATGTGGTCAAGGTTATAGCACAAAACAACTCCAACAAGTGTCTGGAGATAGCAACACTTGCAATTCTGCAAACAAATTTGACACAGTTTTCAATCTAAACTACCCTTCATTTGCTCTTTCCACAACAATCTCAACCTCATTCAGCCAAGTTTACAAAAGAAGGGTCACAAATGTTGGGTCAGCAAACTCAACATACAAAGCTTCAATATTTGCTCCTTTGGGACTTAATATTATAGTGAATCCTTCTGTACTTTCATTCAAAGCTTTGGGAGAAGAGAAAAACTTTGAGGTTACAATTGAAGGAAGTATTGGTAGTAGCATTGCATCTGGTTCCTTGGTTTGGGATGATGGTCAACACAAAGTCAAAAGTCCTATAATTGTATTTGATGTTAAGACATTTATTAGTAATTAGTGTACTCTTTTGTAATTTTACTCTTGCCAAAAGTCAGTTTGGCCTAGCAATAATTGGCATGTACGTTTGATGGAGATGGGA

Coding sequence (CDS)

ATGACTTATCCAACTTTGATGGGTTTGGAAAGAACATCAAGTCCAAAGAGGAACCTTACCTTTTCCACGAGAAAAATCTGTAAAACCTATTATAAGAATAACATTTACATACATCGTATACATGGGGAGTCATCCCAAAGACAAAATTTCAACTTCATCTCATCACGTGAGAATGCTACAAGAAACTATTGGCAGTTGAGTGAAGCCGAAGCACAAAAAGTTTCAGAAATGGAAGGTGTGATATCTGTTTTTCAAAATGAAAGAAAAGAATTGCACACAACAAGATCATGGGACTTCATGGGTCTGTCTCAACAAGCTAGTCGAGTTCCATCTATAGAAAGTGATATCATTGTTGGAGTATTTGACTCAGGCATTTGGCCCGAATCACCTAGCTTTGACGACAAAGGATATAGCCCACCACCATCTAAGTGGAAGGGTACTTGTGAAGTCTCGGCCAATTTCTCTTGTAACAATAAAATTATTGGAGCTCGATCATATCGCATCAATGGAATGTATCCAAAAACAGATATCCAAAGTCCAAGAGATTCACATGGCCATGGTACACACACAGCATCCACAGTGGCAGGCGGGTTGGTTGAGCGAGCGAGCATGCTCGGTCTCGGCATGGGAACAGCAAGGGGAGGAGTCCCATCGGCTCGAATTGCTGCCTACAAAATATGTTGGTCCGATGGCTGCTCTGATGCCGACATTCTTGCTGCATTCGACGATGCCATTGCCGATGGGGTCGACATCATCTCTTTCTCGGTTGGTGGAAACAGCTCGAAAGATTACTTTAAAGACTCCATGGCCATTGGAGCTTTCCATGCAATGAAGAAAGGAATCCTGACGTCAATGTCTGCTGGGAACAGCGGCCCAGGCCCTTCCACAATTAGAAACTTCTCGCCATGGTCTTTGTCTGTGGCGGCCAGTACTACAGATAGAAGATTTTTTACTCGAGTTCAACTTGGAGATGGAACAAGTTTCATGGGAGTCACCATTAATACATTTGATCTAAATGGAACACAATATCCATTGGTATATGCCGGAGATATTCCAAATATTACTGCTGGCTTTGATAAATCAATGTCCAGATTGTGCTTGGAAAATTCAGTGGACAGGGAGTTAGTGAGGGGTAAAATTGCCCTTTGTGATAAAATTTTATCACCAAAAAATGTGAGTTCTTTAAAAGGTGCAATTGGGATTGTAATGCAAGACAATCGTTCAAAAGATCGCACATCCTCTTTTCCATTGCCTGCCTCCCACCTTGACACACAAGGACGAGAAGCCATTTCTTCTTATCTCACCTTAACTAGCCTTCCAACAGCAACTATATTAAAAAGTACAGAAGGGAAAACATCTACTTTTGTTGCATCTTTCTCTTCAAGGGGTCCAAATCCAGTAACACTTGACATTCTTAAGCCCGATTTAACTGGTCCAGGAGTTGAAATTCTTGCAGCATGGTCTCCTATATCTCCACCTTCAGGAGCTGAAGATGATAATAGACATATTTTGTTTAATATCATTTCAGGAACTTCAATGTCTTGTCCACATGCTACCGCAGTTGCTGCATATGTTAAATCATTTCATCCTTCTTGGTCTCCTGCTGCACTTAAATCAGCTCTTATGACAACAGCATTTCCCATGCAAGCAGAGCTAAACAAAGATGCAGAATTTGCATATGGTTCAGGCCATATAAATCCATTAAGCGCAATAAACCCTGGATTAATCTACAATGCTACAGAAATAGACTATATAAAGTTTCTATGTGGTCAAGGTTATAGCACAAAACAACTCCAACAAGTGTCTGGAGATAGCAACACTTGCAATTCTGCAAACAAATTTGACACAGTTTTCAATCTAAACTACCCTTCATTTGCTCTTTCCACAACAATCTCAACCTCATTCAGCCAAGTTTACAAAAGAAGGGTCACAAATGTTGGGTCAGCAAACTCAACATACAAAGCTTCAATATTTGCTCCTTTGGGACTTAATATTATAGTGAATCCTTCTGTACTTTCATTCAAAGCTTTGGGAGAAGAGAAAAACTTTGAGGTTACAATTGAAGGAAGTATTGGTAGTAGCATTGCATCTGGTTCCTTGGTTTGGGATGATGGTCAACACAAAGTCAAAAGTCCTATAATTGTATTTGATGTTAAGACATTTATTAGTAATTAG

Protein sequence

MTYPTLMGLERTSSPKRNLTFSTRKICKTYYKNNIYIHRIHGESSQRQNFNFISSRENATRNYWQLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSGIWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSHGHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDAIADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWSLSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSRLCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDTQGREAISSYLTLTSLPTATILKSTEGKTSTFVASFSSRGPNPVTLDILKPDLTGPGVEILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVSGDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTFISN
Homology
BLAST of Sed0015561.1 vs. NCBI nr
Match: XP_023550545.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1060.1 bits (2740), Expect = 8.8e-306
Identity = 536/661 (81.09%), Postives = 585/661 (88.50%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E QKVSEM+GVISVF N +K+LHTTRSWDFMGLSQQASRVPS+ESDIIVGV D+G
Sbjct: 80  KLTEVEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D+GY PPP KWKG+CEVS++FSCNNKIIGARSYR NG YP  DIQ PRDS+
Sbjct: 140 IWPESPSFLDQGYGPPPRKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTH ASTVAGGLV +ASMLGLG GTARGGVPSARIA+YKICWSDGC DAD+LAAFDDA
Sbjct: 200 GHGTHAASTVAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDGCRDADVLAAFDDA 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIISFSVGG   +DYF DS+AIGAFHAMKKGILTSMSAGNSGP   T+RNFSPWS
Sbjct: 260 IADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSMSAGNSGPKSFTVRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAASTTDR+F + VQLGDG SF GVTINTFDLNGTQYPLVYAG+IPNI  GF+ S+SR
Sbjct: 320 LSVAASTTDRKFLSGVQLGDGRSFDGVTINTFDLNGTQYPLVYAGNIPNI--GFNGSISR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            CL NSVD+ELV+GKI LCD  +SP N+S L+GAIGI+MQDN  KD T  FPLPASHL T
Sbjct: 380 FCLTNSVDKELVKGKIVLCDFFVSPTNLSFLEGAIGIIMQDNNPKDLTFPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LTSLPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QEGALISSYANLTSLPTATILKSTEGKYKETPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAWSPI PPSGAEDD R +LFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM
Sbjct: 500 ILAAWSPIGPPSGAEDDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+A+LN DAEFAYGSGHINPLSA+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C S    D VF+LNYPSFALST+ISTS SQVY+RRVTNVGSANSTYKA +  PLG
Sbjct: 620 GDNSSC-SRGDIDLVFDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVSGPLG 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTFI 724
           LNI VNPSVLSFKALGEE  FEVTIEGSI SSIASGSLVWDDG+HKVKSPI+VFD  TFI
Sbjct: 680 LNITVNPSVLSFKALGEELRFEVTIEGSISSSIASGSLVWDDGKHKVKSPIVVFDENTFI 737

BLAST of Sed0015561.1 vs. NCBI nr
Match: XP_022922167.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.5e-302
Identity = 527/661 (79.73%), Postives = 580/661 (87.75%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E + +SEM+GVISVF NE+K+LHTTRSWDFMGLSQQ  RVPS+ESDIIVGV D+G
Sbjct: 80  KLTEDEVKIISEMKGVISVFPNEKKQLHTTRSWDFMGLSQQVGRVPSVESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D+GY PPP +WKG+CE S NFSCNNKIIGARSYR NG YP  DIQ PRDS+
Sbjct: 140 IWPESPSFLDEGYGPPPPRWKGSCEASLNFSCNNKIIGARSYRTNGQYPINDIQGPRDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTHTASTVAGGLV +ASMLGLG GTARGGVPSARIA+YKICWSD CSDADILAAFDDA
Sbjct: 200 GHGTHTASTVAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDDCSDADILAAFDDA 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIISFSVGG++ KDYF DSMAIGAFHAMKKGILTSMSAGN GP   TIRNFSPWS
Sbjct: 260 IADGVDIISFSVGGHTPKDYFNDSMAIGAFHAMKKGILTSMSAGNDGPQSFTIRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAASTT+R + + +QLGDG SF GVTINTFDLNGTQYPLVYAG+IPNIT GF+ S+SR
Sbjct: 320 LSVAASTTNRSYLSGIQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNITGGFNGSISR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            CL NSVDRE V+GKI LCD  +SPKN+ SL+GAIGI+MQD   KD T  FPLPASHL T
Sbjct: 380 FCLPNSVDRESVKGKIVLCDFFVSPKNLGSLEGAIGIIMQDTNPKDLTFPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LT LPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QEGALISSYANLTGLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAWSPI PPSGAE+D R +LFNIISGTSMSCPHATAVAAYVK+FHPSWSPAALKSALM
Sbjct: 500 ILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+A+LN DAEFAYGSGHINPLSA+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C S    D VF+LNYPSFALST+ISTS SQVY+RRVTNVGSANSTYKA +  P G
Sbjct: 620 GDNSSC-SRGDIDLVFDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVSGPSG 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTFI 724
           LNI VNPSVLSFKALGEE +FE+TIEGSI SSIAS SLVWDDG+HKVKSPI+VFD  TFI
Sbjct: 680 LNITVNPSVLSFKALGEELSFELTIEGSISSSIASASLVWDDGKHKVKSPIVVFDENTFI 739

BLAST of Sed0015561.1 vs. NCBI nr
Match: KAG6579374.1 (hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1038.5 bits (2684), Expect = 2.7e-299
Identity = 523/661 (79.12%), Postives = 579/661 (87.59%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E QKVSEM+GVISVF N +K+LHTTRSWDFMGLSQQASRVPS+ESDIIVGV D+G
Sbjct: 80  KLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D+GY PPP KWKG+CEVS++FSCNNKIIGARSYR NG YP  DIQ PRDS+
Sbjct: 140 IWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTH ASTVAG LV +ASMLGLG GTARGGVPSARIA+YKICWSDGC DAD+LAAFDDA
Sbjct: 200 GHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYKICWSDGCRDADVLAAFDDA 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIISFSVGG   +DYF DS+AIGAFHAMKKGILTSMSAGNSGP   T+RNFSPWS
Sbjct: 260 IADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSMSAGNSGPKSFTVRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAASTTDR+  T VQLGDG SF GVT+NTFDLNGTQYPLVYAG+IPN+  GF+ S+SR
Sbjct: 320 LSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPLVYAGNIPNV--GFNGSISR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            CL NSVD+E V+GKI LCD  +SP N+S L+GAIGI+MQDN  KD T  FPLPASHL T
Sbjct: 380 FCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNNPKDLTFPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LTSLPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAWSPI PPSGAE+D R +LFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM
Sbjct: 500 ILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+++LN DAEFAYGSGHINPL A+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C+ ++  D VF+LNYPSFALST ISTS SQVY+R+VTNVGSANS YKA++  P G
Sbjct: 620 GDNSSCSRSDS-DVVFDLNYPSFALSTPISTSISQVYRRKVTNVGSANSIYKATVSGPSG 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTFI 724
           L I VNPSVLSFKALGEE +FEVTIEGSI SSIAS SLVWDDGQHKV+SPIIVFD   FI
Sbjct: 680 LKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQHKVRSPIIVFDSNKFI 737

BLAST of Sed0015561.1 vs. NCBI nr
Match: XP_022973015.1 (cucumisin-like [Cucurbita maxima])

HSP 1 Score: 1031.9 bits (2667), Expect = 2.6e-297
Identity = 520/659 (78.91%), Postives = 573/659 (86.95%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E QKVSEM+GVISVF N +K+LHTTRSWDFMGLSQQ SRVP +ESDIIVGV D+G
Sbjct: 80  KLTEDEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQVSRVPLVESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D+GY PPP KWKG+CE S NFSCNNKIIGARSYR +G Y   DIQ P DS+
Sbjct: 140 IWPESPSFLDEGYGPPPPKWKGSCETSLNFSCNNKIIGARSYRTSGNYAIGDIQGPIDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTHTASTVAGGLV +ASMLGLG GTARGGVPSARIA+YKICWSDGCSDADILAAFDDA
Sbjct: 200 GHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYKICWSDGCSDADILAAFDDA 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIISFSVGG++  +YF DS+AIGAFHAMK GILTSMSAGN G  P TIRNFSPWS
Sbjct: 260 IADGVDIISFSVGGHTPNNYFNDSIAIGAFHAMKNGILTSMSAGNDGWKPFTIRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAASTTDRRF ++VQLGDG SF GVTINTFDLNGTQYPLVYAG+IPN++AGF+ S+SR
Sbjct: 320 LSVAASTTDRRFLSKVQLGDGRSFDGVTINTFDLNGTQYPLVYAGNIPNVSAGFNGSISR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            CL NSVD +LV+GKI LCD  +SPK    L+GAIGI+MQDNR KD T  FPLPASHL T
Sbjct: 380 FCLRNSVDSDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQDNRPKDLTFPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LTSLPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QQGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAW+P+ PPSGAE+D R +LFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM
Sbjct: 500 ILAAWTPLGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+A+LN DAEFAYGSGHINPL A+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRADLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C S   FD VF+LNYPSFALST +STS SQVY+RRVTNVGSANSTYKA +  P G
Sbjct: 620 GDNSSC-SRGDFDLVFDLNYPSFALSTPMSTSISQVYRRRVTNVGSANSTYKAIVSGPSG 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTF 722
           L I +NPSVLSFKALGEE +FE+TIEGSI   IAS SLVWDDGQH VKSPI+VFD  TF
Sbjct: 680 LKITLNPSVLSFKALGEELSFELTIEGSISRGIASASLVWDDGQHNVKSPIVVFDPNTF 737

BLAST of Sed0015561.1 vs. NCBI nr
Match: XP_023551327.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1029.2 bits (2660), Expect = 1.7e-296
Identity = 520/659 (78.91%), Postives = 573/659 (86.95%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E QKVSEM+GVISVF N +K+LHTTRSWDFMGLSQQ SRVPSIESDIIVGV D+G
Sbjct: 80  KLTEDEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQVSRVPSIESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D+GY  PP KWKG+CE S NFSCNNKIIGARSYR +G Y   DIQ P DS+
Sbjct: 140 IWPESPSFLDEGYGRPPPKWKGSCEASLNFSCNNKIIGARSYRTSGSYVIGDIQGPIDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTHTASTVAGGLV +ASMLGLG GTARGGVPSARIA+YKICWSDGC +ADILAAFDDA
Sbjct: 200 GHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYKICWSDGCPEADILAAFDDA 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIIS SVGG++  +YF DS+AIGAFHAMK GILTSMSAGN G  P TIRNFSPWS
Sbjct: 260 IADGVDIISLSVGGHTPNNYFNDSIAIGAFHAMKNGILTSMSAGNDGWKPFTIRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAASTTDRRF ++VQLGDG SF GVTINTFDLNGTQYPLVYAG+IPN+TAGF+ S+SR
Sbjct: 320 LSVAASTTDRRFLSKVQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNVTAGFNGSISR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            C  NSVDR+L++GKI LCD  +SPK    LKGAIGI+MQDN  KD +S FPLPASHL T
Sbjct: 380 FCTRNSVDRDLMKGKIVLCDHFVSPKKTIFLKGAIGIIMQDNNPKDLSSPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LTSLPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QEGALISSYANLTSLPTATILKSTEGKYKETPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAWSP+ PPSGA++D+R +LFNIISGTSM+CPHATAVAAYVKSFHPSWSPAALKSALM
Sbjct: 500 ILAAWSPLGPPSGAKEDSRQLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+A+LN +AEFAYGSGHINPLSA+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRADLNPEAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C S   FD VF+LNYPSFALST ISTS SQVY+RRVTNVGSANSTY A +  P G
Sbjct: 620 GDNSSC-SRGDFDLVFDLNYPSFALSTPISTSISQVYRRRVTNVGSANSTYNAIVSGPSG 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTF 722
           L I VNPSVLSF ALGEE +FEVTIEGSI   IASGSLVWDDGQHKVKSPI+VFD  TF
Sbjct: 680 LKITVNPSVLSFNALGEELSFEVTIEGSISRGIASGSLVWDDGQHKVKSPIVVFDPNTF 737

BLAST of Sed0015561.1 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 5.1e-216
Identity = 382/654 (58.41%), Postives = 477/654 (72.94%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E EA+K++ MEGV+SVF NE  ELHTTRSWDF+G      R   +ES+I+VGV D+G
Sbjct: 83  KLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTG 142

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSFDD+G+SPPP KWKGTCE S NF CN KIIGARSY I       D+  PRD++
Sbjct: 143 IWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTN 202

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTHTAST AGGLV +A++ GLG+GTARGGVP ARIAAYK+CW+DGCSD DILAA+DDA
Sbjct: 203 GHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDA 262

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIIS SVGG + + YF D++AIG+FHA+++GILTS SAGN GP   T  + SPW 
Sbjct: 263 IADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWL 322

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAAST DR+F T+VQ+G+G SF GV+INTFD     YPLV   DIPN   GFDKS SR
Sbjct: 323 LSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPN--TGFDKSTSR 382

Query: 365 LCLENSVDRELVRGKIALCDKILSPKN-VSSLKGAIGIVMQDNRSKDRTSSFPLPASHLD 424
            C + SV+  L++GKI +C+    P     SL GA G++M  N ++D   S+PLP+S LD
Sbjct: 383 FCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSN-TRDYADSYPLPSSVLD 442

Query: 425 TQGREAISSYLTLTSLPTATILKSTE--GKTSTFVASFSSRGPNPVTLDILKPDLTGPGV 484
                A   Y+     P ATI KST     ++  V SFSSRGPN  T D++KPD++GPGV
Sbjct: 443 PNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGV 502

Query: 485 EILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSAL 544
           EILAAW  ++P  G     R+ LFNIISGTSMSCPH T +A YVK+++P+WSPAA+KSAL
Sbjct: 503 EILAAWPSVAPVGGI---RRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSAL 562

Query: 545 MTTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQV 604
           MTTA PM A  N  AEFAYGSGH+NPL A+ PGL+Y+A E DY+KFLCGQGY+T+ ++++
Sbjct: 563 MTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRI 622

Query: 605 SGDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPL 664
           +GD + C S N    V++LNYPSF LS + S +F+Q + R +T+V    STY+A I AP 
Sbjct: 623 TGDYSACTSGNT-GRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQ 682

Query: 665 GLNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIV 716
           GL I VNP+VLSF  LG+ K+F +T+ GSI   + S SLVW DG H V+SPI +
Sbjct: 683 GLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITI 727

BLAST of Sed0015561.1 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.5e-175
Identity = 327/662 (49.40%), Postives = 440/662 (66.47%), Query Frame = 0

Query: 66  LSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSGI 125
           LS+AE+QK+  M+ V+SVF ++  EL TTRSWDF+G  ++A R    ESD+IVGV DSGI
Sbjct: 83  LSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGI 142

Query: 126 WPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSHG 185
           WPES SFDD+G+ PPP KWKG+C+    F+CNNK+IGAR Y            S RD  G
Sbjct: 143 WPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY-------NKFADSARDEEG 202

Query: 186 HGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDAI 245
           HGTHTAST AG  V+ AS  GL  GTARGGVPSARIAAYK+C+ + C+D DILAAFDDAI
Sbjct: 203 HGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAI 262

Query: 246 ADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWSL 305
           ADGVD+IS S+  +   +    S+AIG+FHAM +GI+T+ SAGN+GP   ++ N SPW +
Sbjct: 263 ADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMI 322

Query: 306 SVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSRL 365
           +VAAS TDR+F  RV LG+G +  G+++NTF+LNGT++P+VY     N++    ++ +  
Sbjct: 323 TVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYG---QNVSRNCSQAQAGY 382

Query: 366 CLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDTQ 425
           C    VD ELV+GKI LCD  L  +  + L GAIG+++Q+    D     P PAS L  +
Sbjct: 383 CSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFE 442

Query: 426 GREAISSYLTLTSLPTATILKSTE--GKTSTFVASFSSRGPNPVTLDILKPDLTGPGVEI 485
             ++I SY+     P A IL++ E   + + +V SFSSRGP+ V  ++LKPD++ PG+EI
Sbjct: 443 DYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEI 502

Query: 486 LAAWSPISPPSG--AEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSAL 545
           LAA+SP++ PS     +D R + ++++SGTSM+CPH   VAAYVKSFHP WSP+A+KSA+
Sbjct: 503 LAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAI 562

Query: 546 MTTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQV 605
           MTTA PM  + N + EFAYGSG INP  A +PGL+Y     DY+K LC +G+ +  L   
Sbjct: 563 MTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTT 622

Query: 606 SGDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPL 665
           SG + TC+   +   V +LNYP+     +    F+  +KR VTNVG  NSTYKAS+  PL
Sbjct: 623 SGQNVTCSERTE---VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASV-VPL 682

Query: 666 --GLNIIVNPSVLSFKALGEEKNFEVTIEGS--IGSSIASGSLVWDDGQHKVKSPIIVFD 720
              L I + P +L F  L E+K+F VTI G      S  S S+VW DG H V+SPI+ + 
Sbjct: 683 QPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 728

BLAST of Sed0015561.1 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 7.0e-173
Identity = 328/683 (48.02%), Postives = 459/683 (67.20%), Query Frame = 0

Query: 45  SQRQNFNFISSRENATRNYWQLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQ 104
           S +++FN  ++R         L+E+E ++V++M GV+SVF N++ +L TT SWDFMGL +
Sbjct: 71  SYKRSFNGFAAR---------LTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKE 130

Query: 105 --QASRVPSIESDIIVGVFDSGIWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIG 164
             +  R P++ESD I+GV DSGI PES SF DKG+ PPP KWKG C    NF+CNNK+IG
Sbjct: 131 GIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIG 190

Query: 165 ARSYRINGMYPKTDIQSPRDSHGHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIA 224
           AR Y   G          RD  GHGTHTAST AG  V  AS  G+G GT RGGVP++R+A
Sbjct: 191 ARDYTSEG---------TRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVA 250

Query: 225 AYKICWSDGCSDADILAAFDDAIADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGIL 284
           AYK+C   GCS   +L+AFDDAIADGVD+I+ S+G  ++  +  D +AIGAFHAM KG+L
Sbjct: 251 AYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVL 310

Query: 285 TSMSAGNSGPGPSTIRNFSPWSLSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQ 344
           T  SAGNSGP P ++   +PW L+VAASTT+R F T+V LG+G + +G ++N +++ G  
Sbjct: 311 TVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKD 370

Query: 345 YPLVYAGDIPNITAGFDKSMSRLCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIV 404
           YPLVY       ++  D   + LC  + VD+  V+GKI +C      K V S+ GA+G++
Sbjct: 371 YPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVGLI 430

Query: 405 MQDNRSKDRTSSFPLPASHLDTQGREAISSYLTLTSLPTATILKSTE--GKTSTFVASFS 464
            +  +  D     PLPA+ L T+  E++ SYL  T  P A +LK+     +TS  +ASFS
Sbjct: 431 YRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFS 490

Query: 465 SRGPNPVTLDILKPDLTGPGVEILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATA 524
           SRGPN + +DILKPD+T PGVEILAA+SP   PS  +DD RH+ ++++SGTSMSCPH   
Sbjct: 491 SRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVAG 550

Query: 525 VAAYVKSFHPSWSPAALKSALMTTAFPMQAELN--KDAEFAYGSGHINPLSAINPGLIYN 584
           VAAYVK+F+P WSP+ ++SA+MTTA+P+ A        EFAYGSGH++P++A NPGL+Y 
Sbjct: 551 VAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYE 610

Query: 585 ATEIDYIKFLCGQGYSTKQLQQVSGDSNTCNSANKFDTVFNLNYPSFALSTTIS-TSFSQ 644
             + D+I FLCG  Y+++ L+ +SG++ TC+ A K     NLNYPS +   + S T+F+ 
Sbjct: 611 LDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKI-LPRNLNYPSMSAKLSGSGTTFTV 670

Query: 645 VYKRRVTNVGSANSTYKASIFAPLG--LNIIVNPSVLSFKALGEEKNFEVTIEGS-IGSS 704
            + R +TNVG+ NSTY + + A  G  L++ + PSVLSFK + E+++F VT+ GS + S 
Sbjct: 671 TFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSE 728

Query: 705 I-ASGSLVWDDGQHKVKSPIIVF 717
           + +S +L+W DG H V+SPI+V+
Sbjct: 731 VPSSANLIWSDGTHNVRSPIVVY 728

BLAST of Sed0015561.1 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.9e-170
Identity = 330/684 (48.25%), Postives = 453/684 (66.23%), Query Frame = 0

Query: 45  SQRQNFNFISSRENATRNYWQLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQ 104
           S +++FN  ++R         L+E+E   ++E+EGV+SVF N+  +LHTT SWDFMG+ +
Sbjct: 72  SYKRSFNGFAAR---------LTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKE 131

Query: 105 --QASRVPSIESDIIVGVFDSGIWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIG 164
                R  +IESD I+GV D+GIWPES SF DKG+ PPP KWKG C    NF+CNNK+IG
Sbjct: 132 GKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIG 191

Query: 165 ARSYRINGMYPKTDIQSPRDSHGHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIA 224
           AR Y   G          RD+ GHGTHTAST AG  V+  S  G+G GT RGGVP++RIA
Sbjct: 192 ARDYTSEG---------TRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIA 251

Query: 225 AYKICWSDGCSDADILAAFDDAIADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGIL 284
           AYK+C   GCS   +L++FDDAIADGVD+I+ S+G      +  D +AIGAFHAM KGIL
Sbjct: 252 AYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGIL 311

Query: 285 TSMSAGNSGPGPSTIRNFSPWSLSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQ 344
           T  SAGNSGP P+T+ + +PW  +VAASTT+R F T+V LG+G +  G ++N FD+ G +
Sbjct: 312 TVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKK 371

Query: 345 YPLVYAGDIPNITAGFDKSMSRLCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIV 404
           YPLVY       ++  D   + LC    +++  V+GKI +C      K   S+ GAI I+
Sbjct: 372 YPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSV-GAIAII 431

Query: 405 MQDNRSKDRTSSFPLPASHLDTQGREAISSYLTLTSLPTATILKSTE--GKTSTFVASFS 464
            +  R  D   +  LPAS L  +  +++ SY+     P A +LK+     +TS  +ASFS
Sbjct: 432 DKSPR-PDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFS 491

Query: 465 SRGPNPVTLDILKPDLTGPGVEILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATA 524
           SRGPN + +DILKPD+T PGVEILAA+SP   PS  EDD R + +++ SGTSM+CPH   
Sbjct: 492 SRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAG 551

Query: 525 VAAYVKSFHPSWSPAALKSALMTTAFPMQAELN--KDAEFAYGSGHINPLSAINPGLIYN 584
           VAAYVK+F+P WSP+ ++SA+MTTA+P++A+       EFAYG+GH++P++A+NPGL+Y 
Sbjct: 552 VAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYE 611

Query: 585 ATEIDYIKFLCGQGYSTKQLQQVSGDSNTCNSANKFDTVFNLNYPSFA--LSTTISTSFS 644
             + D+I FLCG  Y++K L+ +SGD+  C+  NK     NLNYPS +  LS T ST FS
Sbjct: 612 LDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKI-LPRNLNYPSMSAKLSGTDST-FS 671

Query: 645 QVYKRRVTNVGSANSTYKASIFAPLG--LNIIVNPSVLSFKALGEEKNFEVTIEGS-IGS 704
             + R +TNVG+ NSTYK+ + A  G  L+I V PSVL FK + E+++F VT+ GS + S
Sbjct: 672 VTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDS 729

Query: 705 SI-ASGSLVWDDGQHKVKSPIIVF 717
            + +S +L+W DG H V+SPI+V+
Sbjct: 732 EVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Sed0015561.1 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 9.5e-170
Identity = 326/682 (47.80%), Postives = 448/682 (65.69%), Query Frame = 0

Query: 45  SQRQNFNFISSRENATRNYWQLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQ 104
           S +++FN  ++R         L+E+E ++++ ME V+SVF + + +L TT SW+FMGL +
Sbjct: 74  SYKKSFNGFAAR---------LTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKE 133

Query: 105 --QASRVPSIESDIIVGVFDSGIWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIG 164
             +  R  SIESD I+GV DSGI+PES SF D+G+ PPP KWKGTC    NF+CNNK+IG
Sbjct: 134 GIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIG 193

Query: 165 ARSYRINGMYPKTDIQSPRDSHGHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIA 224
           AR Y       K + Q+ RD  GHGTHTAS  AG  V  ++  GLG GTARGGVP+ARIA
Sbjct: 194 ARDYTAKS---KAN-QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIA 253

Query: 225 AYKICWSDGCSDADILAAFDDAIADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGIL 284
            YK+C ++GC    +++AFDDAIADGVD+IS S+  ++   + +D +AIGAFHAM  G+L
Sbjct: 254 VYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVL 313

Query: 285 TSMSAGNSGPGPSTIRNFSPWSLSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQ 344
           T  +AGN+GP  ST+ + +PW  SVAAS T+R F  +V LGDG   +G ++NT+D+NGT 
Sbjct: 314 TVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTN 373

Query: 345 YPLVYAGDIPNITAGFDKSMSRLCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIV 404
           YPLVY       T   DK  +RLC    +D +LV+GKI LCD          L GA+G +
Sbjct: 374 YPLVYGKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL-GAVGSI 433

Query: 405 MQDNRSKDRTSSFPLPASHLDTQGREAISSYLTLTSLPTATILKSTE--GKTSTFVASFS 464
           ++ N   DR      P S L     +++ SY+  T  P AT+LKS E   + +  VASFS
Sbjct: 434 VK-NPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFS 493

Query: 465 SRGPNPVTLDILKPDLTGPGVEILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATA 524
           SRGP+ +  DILKPD+T PGVEILAA+SP S P+ +E D R + ++++SGTSM+CPH   
Sbjct: 494 SRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAG 553

Query: 525 VAAYVKSFHPSWSPAALKSALMTTAFPMQAELN--KDAEFAYGSGHINPLSAINPGLIYN 584
           VAAYVK+FHP WSP+ ++SA+MTTA+PM A  +     EFAYGSGH++P+ AINPGL+Y 
Sbjct: 554 VAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYE 613

Query: 585 ATEIDYIKFLCGQGYSTKQLQQVSGDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQV 644
            T+ D+I FLCG  Y++  L+ +SGD++TC          NLNYP+ +   + +  F+  
Sbjct: 614 LTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNIT 673

Query: 645 YKRRVTNVGSANSTYKASI--FAPLGLNIIVNPSVLSFKALGEEKNFEVTIEG-SIGS-S 704
           ++R VTNVG   STY A +  F    L+I V+P VLS K++ E+++F VT+   SIG+  
Sbjct: 674 FQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQ 733

Query: 705 IASGSLVWDDGQHKVKSPIIVF 717
             S +L+W DG H V+SPIIV+
Sbjct: 734 PVSANLIWSDGTHNVRSPIIVY 738

BLAST of Sed0015561.1 vs. ExPASy TrEMBL
Match: A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 7.5e-303
Identity = 527/661 (79.73%), Postives = 580/661 (87.75%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E + +SEM+GVISVF NE+K+LHTTRSWDFMGLSQQ  RVPS+ESDIIVGV D+G
Sbjct: 80  KLTEDEVKIISEMKGVISVFPNEKKQLHTTRSWDFMGLSQQVGRVPSVESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D+GY PPP +WKG+CE S NFSCNNKIIGARSYR NG YP  DIQ PRDS+
Sbjct: 140 IWPESPSFLDEGYGPPPPRWKGSCEASLNFSCNNKIIGARSYRTNGQYPINDIQGPRDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTHTASTVAGGLV +ASMLGLG GTARGGVPSARIA+YKICWSD CSDADILAAFDDA
Sbjct: 200 GHGTHTASTVAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDDCSDADILAAFDDA 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIISFSVGG++ KDYF DSMAIGAFHAMKKGILTSMSAGN GP   TIRNFSPWS
Sbjct: 260 IADGVDIISFSVGGHTPKDYFNDSMAIGAFHAMKKGILTSMSAGNDGPQSFTIRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAASTT+R + + +QLGDG SF GVTINTFDLNGTQYPLVYAG+IPNIT GF+ S+SR
Sbjct: 320 LSVAASTTNRSYLSGIQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNITGGFNGSISR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            CL NSVDRE V+GKI LCD  +SPKN+ SL+GAIGI+MQD   KD T  FPLPASHL T
Sbjct: 380 FCLPNSVDRESVKGKIVLCDFFVSPKNLGSLEGAIGIIMQDTNPKDLTFPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LT LPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QEGALISSYANLTGLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAWSPI PPSGAE+D R +LFNIISGTSMSCPHATAVAAYVK+FHPSWSPAALKSALM
Sbjct: 500 ILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+A+LN DAEFAYGSGHINPLSA+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C S    D VF+LNYPSFALST+ISTS SQVY+RRVTNVGSANSTYKA +  P G
Sbjct: 620 GDNSSC-SRGDIDLVFDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVSGPSG 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTFI 724
           LNI VNPSVLSFKALGEE +FE+TIEGSI SSIAS SLVWDDG+HKVKSPI+VFD  TFI
Sbjct: 680 LNITVNPSVLSFKALGEELSFELTIEGSISSSIASASLVWDDGKHKVKSPIVVFDENTFI 739

BLAST of Sed0015561.1 vs. ExPASy TrEMBL
Match: A0A6J1IAA2 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111471532 PE=3 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 1.2e-297
Identity = 520/659 (78.91%), Postives = 573/659 (86.95%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E QKVSEM+GVISVF N +K+LHTTRSWDFMGLSQQ SRVP +ESDIIVGV D+G
Sbjct: 80  KLTEDEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQVSRVPLVESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D+GY PPP KWKG+CE S NFSCNNKIIGARSYR +G Y   DIQ P DS+
Sbjct: 140 IWPESPSFLDEGYGPPPPKWKGSCETSLNFSCNNKIIGARSYRTSGNYAIGDIQGPIDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTHTASTVAGGLV +ASMLGLG GTARGGVPSARIA+YKICWSDGCSDADILAAFDDA
Sbjct: 200 GHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYKICWSDGCSDADILAAFDDA 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIISFSVGG++  +YF DS+AIGAFHAMK GILTSMSAGN G  P TIRNFSPWS
Sbjct: 260 IADGVDIISFSVGGHTPNNYFNDSIAIGAFHAMKNGILTSMSAGNDGWKPFTIRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAASTTDRRF ++VQLGDG SF GVTINTFDLNGTQYPLVYAG+IPN++AGF+ S+SR
Sbjct: 320 LSVAASTTDRRFLSKVQLGDGRSFDGVTINTFDLNGTQYPLVYAGNIPNVSAGFNGSISR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            CL NSVD +LV+GKI LCD  +SPK    L+GAIGI+MQDNR KD T  FPLPASHL T
Sbjct: 380 FCLRNSVDSDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQDNRPKDLTFPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LTSLPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QQGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAW+P+ PPSGAE+D R +LFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM
Sbjct: 500 ILAAWTPLGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+A+LN DAEFAYGSGHINPL A+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRADLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C S   FD VF+LNYPSFALST +STS SQVY+RRVTNVGSANSTYKA +  P G
Sbjct: 620 GDNSSC-SRGDFDLVFDLNYPSFALSTPMSTSISQVYRRRVTNVGSANSTYKAIVSGPSG 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTF 722
           L I +NPSVLSFKALGEE +FE+TIEGSI   IAS SLVWDDGQH VKSPI+VFD  TF
Sbjct: 680 LKITLNPSVLSFKALGEELSFELTIEGSISRGIASASLVWDDGQHNVKSPIVVFDPNTF 737

BLAST of Sed0015561.1 vs. ExPASy TrEMBL
Match: A0A6J1E2C5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 1.8e-296
Identity = 518/661 (78.37%), Postives = 577/661 (87.29%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E QKVSEM+GVISVF N +K+LHTTRSWDFMGLSQQASRVPS+ESDIIVGV D+G
Sbjct: 80  KLTEVEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D+GY PPP KWKG+CEVS++FSCNNKIIGARSYR NG YP  DIQ PRDS+
Sbjct: 140 IWPESPSFLDQGYGPPPRKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTH ASTVAGGLV +ASMLGLG GTARGGVPSARIA+YKICWSDGC DADILAAFDDA
Sbjct: 200 GHGTHAASTVAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDGCPDADILAAFDDA 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIISFSVGG + +DYF DS+AIGAFHAMKK ILTSMSAGN+GP   T+RNFSPWS
Sbjct: 260 IADGVDIISFSVGGQTPRDYFNDSIAIGAFHAMKKEILTSMSAGNNGPKSFTVRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVAASTTDR+F T VQLGDG SF GVTINTFDLNGTQYPLVYAG+IPNI  GF+ S+SR
Sbjct: 320 LSVAASTTDRKFLTGVQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNI--GFNGSISR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            CL NSVD+E V+GKI LCD  + P N++ L+GAIGI+MQDN  KD T  FPLPASHL T
Sbjct: 380 FCLTNSVDKESVKGKIVLCDFFVLPTNLNFLEGAIGIIMQDNNPKDLTFPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LTSLPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAWSPI PPSGAE+D R +LFNIISGTSMSCPHATAVAAYVK+FHPSWSPAALKSALM
Sbjct: 500 ILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+++LN DAEFAYGSGHINPL A+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C+ ++  + VF+LNYPSFALST+IST  SQVY+RRVTNVGSANSTY A +  P  
Sbjct: 620 GDNSSCSRSDS-NVVFDLNYPSFALSTSISTPISQVYRRRVTNVGSANSTYNAIVSGPSR 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTFI 724
           L I VNPSVLSFKALGEE +FEVTIEGSI S I S SLVWDDGQHKV+SP+IVFD  +FI
Sbjct: 680 LKITVNPSVLSFKALGEELSFEVTIEGSISSGIVSASLVWDDGQHKVRSPVIVFDSTSFI 737

BLAST of Sed0015561.1 vs. ExPASy TrEMBL
Match: A0A6J1E6I3 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430414 PE=3 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 5.8e-295
Identity = 516/659 (78.30%), Postives = 570/659 (86.49%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E E QKVSEM+GVISVF N +K+LHTTRSWDFMGLSQQ SRVPS+ESDIIVGV D+G
Sbjct: 80  KLTEEEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQVSRVPSVESDIIVGVLDTG 139

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWP SPSF D+GY PPP KWKG+CE S NFSCNNKIIGARSYR +G Y   DIQ P DS+
Sbjct: 140 IWPNSPSFLDEGYGPPPPKWKGSCEASLNFSCNNKIIGARSYRTSGNYAIGDIQGPIDSN 199

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTHTASTVAGGLV +ASMLGLG GTARGGVPSARIA+YKICWSDGC +ADILAAFDD 
Sbjct: 200 GHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYKICWSDGCPEADILAAFDDV 259

Query: 245 IADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWS 304
           IADGVDIIS SVGG++  +YF DS+AIGAFHAMK GILTSMSAGN G  P TIRNFSPWS
Sbjct: 260 IADGVDIISLSVGGHTPNNYFNDSIAIGAFHAMKNGILTSMSAGNDGWKPFTIRNFSPWS 319

Query: 305 LSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSR 364
           LSVA+STT+RRF ++VQLGDG SF GVTINTFDLNGTQY LVYAG+IPN+TAGF+ S+SR
Sbjct: 320 LSVASSTTNRRFLSKVQLGDGRSFHGVTINTFDLNGTQYSLVYAGNIPNVTAGFNGSVSR 379

Query: 365 LCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDT 424
            C  NSVDR+LV+GKI LCD  +SPK    L+GAIGI+MQDN  KD +S FPLPASHL T
Sbjct: 380 FCTRNSVDRDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQDNNPKDLSSPFPLPASHLGT 439

Query: 425 QGREAISSYLTLTSLPTATILKSTEG--KTSTFVASFSSRGPNPVTLDILKPDLTGPGVE 484
           Q    ISSY  LTSLPTATILKSTEG  K + FVASFSSRGPNP+T DILKPDL+GPGVE
Sbjct: 440 QEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVE 499

Query: 485 ILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 544
           ILAAW+P+ PPSGA++D R +LFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM
Sbjct: 500 ILAAWTPLGPPSGAKEDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALM 559

Query: 545 TTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQVS 604
           TTAFPM+AELN +AEFAYGSGHINPLSA+NPGLIYNATEIDY++FLCGQGYSTK +QQVS
Sbjct: 560 TTAFPMRAELNPEAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVS 619

Query: 605 GDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPLG 664
           GD+++C S   FD VF+LNYPSFALST ISTS SQVY+RRVTNVGSANSTY A +  P G
Sbjct: 620 GDNSSC-SRGDFDLVFDLNYPSFALSTPISTSISQVYRRRVTNVGSANSTYNAIVSGPSG 679

Query: 665 LNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTF 722
           L I VNPSVLSF ALGEE +FEVTIEGSI SSIAS SLVWDDG HKVKSPI+VFD  TF
Sbjct: 680 LKITVNPSVLSFNALGEELSFEVTIEGSISSSIASASLVWDDGHHKVKSPIVVFDPNTF 737

BLAST of Sed0015561.1 vs. ExPASy TrEMBL
Match: A0A1S3AUT1 (cucumisin-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 3.7e-278
Identity = 492/660 (74.55%), Postives = 551/660 (83.48%), Query Frame = 0

Query: 65  QLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSG 124
           +L+E EA+KVSEMEGVISVF NE  +LHTTRSWDFMG S+Q  RVPS+ESDIIVGVFD+G
Sbjct: 27  KLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTG 86

Query: 125 IWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSH 184
           IWPESPSF D GY PPP KWKG+CEVS NFSCNNKIIGA+SYR +G YP  DI+ PRDS+
Sbjct: 87  IWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSN 146

Query: 185 GHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDA 244
           GHGTH ASTVAGGLV +ASMLGLG GTARGGVPSARIAAYK+CWSD CS AD+LAAFDDA
Sbjct: 147 GHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDA 206

Query: 245 IADGVDIISFSVGGNSS-KDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPW 304
           IADGVDIIS SVG      +YF D +AIG FHAM+ GILTS SAGN GP P T+ NFSPW
Sbjct: 207 IADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPW 266

Query: 305 SLSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMS 364
           +LSVAASTTDRRF T VQLGDG  F GVTINTFDLNGTQYPLV+AG+IPN+T GF+ S+S
Sbjct: 267 ALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSIS 326

Query: 365 RLCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLD 424
           R CL NSVDRELV+GKIA+CD I+ P +V SL+ A+GI+MQD   KD T++FPLPASHL 
Sbjct: 327 RFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLG 386

Query: 425 TQGREAISSYLTLTSLPTATILKSTEGK--TSTFVASFSSRGPNPVTLDILKPDLTGPGV 484
           TQ R  ISSYL LT +PTATILKSTE K   +  VASFSSRGPNP T DILKPD+ GPGV
Sbjct: 387 TQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGV 446

Query: 485 EILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSAL 544
           EILAAWSPI  PS A+ DNR +LFNIISGTSM+CPHATAVAAYVKSFHPSWSPAALKSAL
Sbjct: 447 EILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSAL 506

Query: 545 MTTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQV 604
           +TTAFPM+ +L  +AEFAYGSGHINPL A+NPGLIYNA+EIDYI+FLCG+GY+T  L+ +
Sbjct: 507 ITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRII 566

Query: 605 SGDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPL 664
           + D++TC+  N    V++LNY SFAL T IST FSQVYKRRVTNVGS NSTYKA+IFAP 
Sbjct: 567 TKDNSTCSPTNS-GLVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYKATIFAPS 626

Query: 665 GLNIIVNPSVLSFKALGEEKNFEVTIEGSIGSSIASGSLVWDDGQHKVKSPIIVFDVKTF 722
            LNI VNPS LSFKAL EE  FEVTIEG I  SIAS SLVWDDG HKV+SPIIVFD  TF
Sbjct: 627 ELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVFDSDTF 685

BLAST of Sed0015561.1 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 617.8 bits (1592), Expect = 1.1e-176
Identity = 327/662 (49.40%), Postives = 440/662 (66.47%), Query Frame = 0

Query: 66  LSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSGI 125
           LS+AE+QK+  M+ V+SVF ++  EL TTRSWDF+G  ++A R    ESD+IVGV DSGI
Sbjct: 47  LSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGI 106

Query: 126 WPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIGARSYRINGMYPKTDIQSPRDSHG 185
           WPES SFDD+G+ PPP KWKG+C+    F+CNNK+IGAR Y            S RD  G
Sbjct: 107 WPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY-------NKFADSARDEEG 166

Query: 186 HGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIAAYKICWSDGCSDADILAAFDDAI 245
           HGTHTAST AG  V+ AS  GL  GTARGGVPSARIAAYK+C+ + C+D DILAAFDDAI
Sbjct: 167 HGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAI 226

Query: 246 ADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGILTSMSAGNSGPGPSTIRNFSPWSL 305
           ADGVD+IS S+  +   +    S+AIG+FHAM +GI+T+ SAGN+GP   ++ N SPW +
Sbjct: 227 ADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMI 286

Query: 306 SVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQYPLVYAGDIPNITAGFDKSMSRL 365
           +VAAS TDR+F  RV LG+G +  G+++NTF+LNGT++P+VY     N++    ++ +  
Sbjct: 287 TVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYG---QNVSRNCSQAQAGY 346

Query: 366 CLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIVMQDNRSKDRTSSFPLPASHLDTQ 425
           C    VD ELV+GKI LCD  L  +  + L GAIG+++Q+    D     P PAS L  +
Sbjct: 347 CSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFE 406

Query: 426 GREAISSYLTLTSLPTATILKSTE--GKTSTFVASFSSRGPNPVTLDILKPDLTGPGVEI 485
             ++I SY+     P A IL++ E   + + +V SFSSRGP+ V  ++LKPD++ PG+EI
Sbjct: 407 DYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEI 466

Query: 486 LAAWSPISPPSG--AEDDNRHILFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSAL 545
           LAA+SP++ PS     +D R + ++++SGTSM+CPH   VAAYVKSFHP WSP+A+KSA+
Sbjct: 467 LAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAI 526

Query: 546 MTTAFPMQAELNKDAEFAYGSGHINPLSAINPGLIYNATEIDYIKFLCGQGYSTKQLQQV 605
           MTTA PM  + N + EFAYGSG INP  A +PGL+Y     DY+K LC +G+ +  L   
Sbjct: 527 MTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTT 586

Query: 606 SGDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQVYKRRVTNVGSANSTYKASIFAPL 665
           SG + TC+   +   V +LNYP+     +    F+  +KR VTNVG  NSTYKAS+  PL
Sbjct: 587 SGQNVTCSERTE---VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASV-VPL 646

Query: 666 --GLNIIVNPSVLSFKALGEEKNFEVTIEGS--IGSSIASGSLVWDDGQHKVKSPIIVFD 720
              L I + P +L F  L E+K+F VTI G      S  S S+VW DG H V+SPI+ + 
Sbjct: 647 QPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 692

BLAST of Sed0015561.1 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 609.0 bits (1569), Expect = 5.0e-174
Identity = 328/683 (48.02%), Postives = 459/683 (67.20%), Query Frame = 0

Query: 45  SQRQNFNFISSRENATRNYWQLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQ 104
           S +++FN  ++R         L+E+E ++V++M GV+SVF N++ +L TT SWDFMGL +
Sbjct: 71  SYKRSFNGFAAR---------LTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKE 130

Query: 105 --QASRVPSIESDIIVGVFDSGIWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIG 164
             +  R P++ESD I+GV DSGI PES SF DKG+ PPP KWKG C    NF+CNNK+IG
Sbjct: 131 GIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIG 190

Query: 165 ARSYRINGMYPKTDIQSPRDSHGHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIA 224
           AR Y   G          RD  GHGTHTAST AG  V  AS  G+G GT RGGVP++R+A
Sbjct: 191 ARDYTSEG---------TRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVA 250

Query: 225 AYKICWSDGCSDADILAAFDDAIADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGIL 284
           AYK+C   GCS   +L+AFDDAIADGVD+I+ S+G  ++  +  D +AIGAFHAM KG+L
Sbjct: 251 AYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVL 310

Query: 285 TSMSAGNSGPGPSTIRNFSPWSLSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQ 344
           T  SAGNSGP P ++   +PW L+VAASTT+R F T+V LG+G + +G ++N +++ G  
Sbjct: 311 TVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKD 370

Query: 345 YPLVYAGDIPNITAGFDKSMSRLCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIV 404
           YPLVY       ++  D   + LC  + VD+  V+GKI +C      K V S+ GA+G++
Sbjct: 371 YPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVGLI 430

Query: 405 MQDNRSKDRTSSFPLPASHLDTQGREAISSYLTLTSLPTATILKSTE--GKTSTFVASFS 464
            +  +  D     PLPA+ L T+  E++ SYL  T  P A +LK+     +TS  +ASFS
Sbjct: 431 YRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFS 490

Query: 465 SRGPNPVTLDILKPDLTGPGVEILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATA 524
           SRGPN + +DILKPD+T PGVEILAA+SP   PS  +DD RH+ ++++SGTSMSCPH   
Sbjct: 491 SRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVAG 550

Query: 525 VAAYVKSFHPSWSPAALKSALMTTAFPMQAELN--KDAEFAYGSGHINPLSAINPGLIYN 584
           VAAYVK+F+P WSP+ ++SA+MTTA+P+ A        EFAYGSGH++P++A NPGL+Y 
Sbjct: 551 VAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYE 610

Query: 585 ATEIDYIKFLCGQGYSTKQLQQVSGDSNTCNSANKFDTVFNLNYPSFALSTTIS-TSFSQ 644
             + D+I FLCG  Y+++ L+ +SG++ TC+ A K     NLNYPS +   + S T+F+ 
Sbjct: 611 LDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKI-LPRNLNYPSMSAKLSGSGTTFTV 670

Query: 645 VYKRRVTNVGSANSTYKASIFAPLG--LNIIVNPSVLSFKALGEEKNFEVTIEGS-IGSS 704
            + R +TNVG+ NSTY + + A  G  L++ + PSVLSFK + E+++F VT+ GS + S 
Sbjct: 671 TFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSE 728

Query: 705 I-ASGSLVWDDGQHKVKSPIIVF 717
           + +S +L+W DG H V+SPI+V+
Sbjct: 731 VPSSANLIWSDGTHNVRSPIVVY 728

BLAST of Sed0015561.1 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 600.9 bits (1548), Expect = 1.4e-171
Identity = 330/684 (48.25%), Postives = 453/684 (66.23%), Query Frame = 0

Query: 45  SQRQNFNFISSRENATRNYWQLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQ 104
           S +++FN  ++R         L+E+E   ++E+EGV+SVF N+  +LHTT SWDFMG+ +
Sbjct: 72  SYKRSFNGFAAR---------LTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKE 131

Query: 105 --QASRVPSIESDIIVGVFDSGIWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIG 164
                R  +IESD I+GV D+GIWPES SF DKG+ PPP KWKG C    NF+CNNK+IG
Sbjct: 132 GKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIG 191

Query: 165 ARSYRINGMYPKTDIQSPRDSHGHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIA 224
           AR Y   G          RD+ GHGTHTAST AG  V+  S  G+G GT RGGVP++RIA
Sbjct: 192 ARDYTSEG---------TRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIA 251

Query: 225 AYKICWSDGCSDADILAAFDDAIADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGIL 284
           AYK+C   GCS   +L++FDDAIADGVD+I+ S+G      +  D +AIGAFHAM KGIL
Sbjct: 252 AYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGIL 311

Query: 285 TSMSAGNSGPGPSTIRNFSPWSLSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQ 344
           T  SAGNSGP P+T+ + +PW  +VAASTT+R F T+V LG+G +  G ++N FD+ G +
Sbjct: 312 TVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKK 371

Query: 345 YPLVYAGDIPNITAGFDKSMSRLCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIV 404
           YPLVY       ++  D   + LC    +++  V+GKI +C      K   S+ GAI I+
Sbjct: 372 YPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSV-GAIAII 431

Query: 405 MQDNRSKDRTSSFPLPASHLDTQGREAISSYLTLTSLPTATILKSTE--GKTSTFVASFS 464
            +  R  D   +  LPAS L  +  +++ SY+     P A +LK+     +TS  +ASFS
Sbjct: 432 DKSPR-PDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFS 491

Query: 465 SRGPNPVTLDILKPDLTGPGVEILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATA 524
           SRGPN + +DILKPD+T PGVEILAA+SP   PS  EDD R + +++ SGTSM+CPH   
Sbjct: 492 SRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAG 551

Query: 525 VAAYVKSFHPSWSPAALKSALMTTAFPMQAELN--KDAEFAYGSGHINPLSAINPGLIYN 584
           VAAYVK+F+P WSP+ ++SA+MTTA+P++A+       EFAYG+GH++P++A+NPGL+Y 
Sbjct: 552 VAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYE 611

Query: 585 ATEIDYIKFLCGQGYSTKQLQQVSGDSNTCNSANKFDTVFNLNYPSFA--LSTTISTSFS 644
             + D+I FLCG  Y++K L+ +SGD+  C+  NK     NLNYPS +  LS T ST FS
Sbjct: 612 LDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKI-LPRNLNYPSMSAKLSGTDST-FS 671

Query: 645 QVYKRRVTNVGSANSTYKASIFAPLG--LNIIVNPSVLSFKALGEEKNFEVTIEGS-IGS 704
             + R +TNVG+ NSTYK+ + A  G  L+I V PSVL FK + E+++F VT+ GS + S
Sbjct: 672 VTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDS 729

Query: 705 SI-ASGSLVWDDGQHKVKSPIIVF 717
            + +S +L+W DG H V+SPI+V+
Sbjct: 732 EVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Sed0015561.1 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 598.6 bits (1542), Expect = 6.7e-171
Identity = 326/682 (47.80%), Postives = 448/682 (65.69%), Query Frame = 0

Query: 45  SQRQNFNFISSRENATRNYWQLSEAEAQKVSEMEGVISVFQNERKELHTTRSWDFMGLSQ 104
           S +++FN  ++R         L+E+E ++++ ME V+SVF + + +L TT SW+FMGL +
Sbjct: 74  SYKKSFNGFAAR---------LTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKE 133

Query: 105 --QASRVPSIESDIIVGVFDSGIWPESPSFDDKGYSPPPSKWKGTCEVSANFSCNNKIIG 164
             +  R  SIESD I+GV DSGI+PES SF D+G+ PPP KWKGTC    NF+CNNK+IG
Sbjct: 134 GIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIG 193

Query: 165 ARSYRINGMYPKTDIQSPRDSHGHGTHTASTVAGGLVERASMLGLGMGTARGGVPSARIA 224
           AR Y       K + Q+ RD  GHGTHTAS  AG  V  ++  GLG GTARGGVP+ARIA
Sbjct: 194 ARDYTAKS---KAN-QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIA 253

Query: 225 AYKICWSDGCSDADILAAFDDAIADGVDIISFSVGGNSSKDYFKDSMAIGAFHAMKKGIL 284
            YK+C ++GC    +++AFDDAIADGVD+IS S+  ++   + +D +AIGAFHAM  G+L
Sbjct: 254 VYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVL 313

Query: 285 TSMSAGNSGPGPSTIRNFSPWSLSVAASTTDRRFFTRVQLGDGTSFMGVTINTFDLNGTQ 344
           T  +AGN+GP  ST+ + +PW  SVAAS T+R F  +V LGDG   +G ++NT+D+NGT 
Sbjct: 314 TVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTN 373

Query: 345 YPLVYAGDIPNITAGFDKSMSRLCLENSVDRELVRGKIALCDKILSPKNVSSLKGAIGIV 404
           YPLVY       T   DK  +RLC    +D +LV+GKI LCD          L GA+G +
Sbjct: 374 YPLVYGKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL-GAVGSI 433

Query: 405 MQDNRSKDRTSSFPLPASHLDTQGREAISSYLTLTSLPTATILKSTE--GKTSTFVASFS 464
           ++ N   DR      P S L     +++ SY+  T  P AT+LKS E   + +  VASFS
Sbjct: 434 VK-NPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFS 493

Query: 465 SRGPNPVTLDILKPDLTGPGVEILAAWSPISPPSGAEDDNRHILFNIISGTSMSCPHATA 524
           SRGP+ +  DILKPD+T PGVEILAA+SP S P+ +E D R + ++++SGTSM+CPH   
Sbjct: 494 SRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAG 553

Query: 525 VAAYVKSFHPSWSPAALKSALMTTAFPMQAELN--KDAEFAYGSGHINPLSAINPGLIYN 584
           VAAYVK+FHP WSP+ ++SA+MTTA+PM A  +     EFAYGSGH++P+ AINPGL+Y 
Sbjct: 554 VAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYE 613

Query: 585 ATEIDYIKFLCGQGYSTKQLQQVSGDSNTCNSANKFDTVFNLNYPSFALSTTISTSFSQV 644
            T+ D+I FLCG  Y++  L+ +SGD++TC          NLNYP+ +   + +  F+  
Sbjct: 614 LTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNIT 673

Query: 645 YKRRVTNVGSANSTYKASI--FAPLGLNIIVNPSVLSFKALGEEKNFEVTIEG-SIGS-S 704
           ++R VTNVG   STY A +  F    L+I V+P VLS K++ E+++F VT+   SIG+  
Sbjct: 674 FQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQ 733

Query: 705 IASGSLVWDDGQHKVKSPIIVF 717
             S +L+W DG H V+SPIIV+
Sbjct: 734 PVSANLIWSDGTHNVRSPIIVY 738

BLAST of Sed0015561.1 vs. TAIR 10
Match: AT5G58830.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 596.3 bits (1536), Expect = 3.3e-170
Identity = 333/691 (48.19%), Postives = 453/691 (65.56%), Query Frame = 0

Query: 37  IHRIHGESSQRQNFNFISSRENATRNYWQLSEAEAQKVSEMEGVISVFQNERKELHTTRS 96
           +  + GES +R +FN  S+          L+E+E + V+EMEGV+SVF+++  +L TT S
Sbjct: 57  LQEVTGESYKR-SFNGFSA---------LLTESEREGVAEMEGVVSVFRSKNYKLQTTAS 116

Query: 97  WDFMGLSQ--QASRVPSIESDIIVGVFDSGIWPESPSFDDKGYSPPPSKWKGTCEVSANF 156
           WDFMG+ +     R  ++ESD I+G  DSGIWPES SF DKG+ PPP KWKG C+   NF
Sbjct: 117 WDFMGMKEGKNTKRNFAVESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCKGGKNF 176

Query: 157 SCNNKIIGARSYRINGMYPKTDIQSPRDSHGHGTHTASTVAGGLVERASMLGLGMGTARG 216
           +CNNK+IGAR Y   G          RD  GHGTHT ST AG  V   S  G+G GTARG
Sbjct: 177 TCNNKLIGARDYTSEG---------TRDLQGHGTHTTSTAAGNAVADTSFFGIGNGTARG 236

Query: 217 GVPSARIAAYKICWSDGCSDADILAAFDDAIADGVDIISFSVGGNSSKDYFKDSMAIGAF 276
           GVP++R+AAYK+C   GCSD ++L+AFDDAIADGVD+IS S+GG+    Y +D++AIGAF
Sbjct: 237 GVPASRVAAYKVCTITGCSDDNVLSAFDDAIADGVDLISVSLGGDYPSLYAEDTIAIGAF 296

Query: 277 HAMKKGILTSMSAGNSGPGPSTIRNFSPWSLSVAASTTDRRFFTRVQLGDGTSFMGVTIN 336
           HAM KGILT  SAGN+GP P+T+ + +PW L+VAA+TT+RRF T+V LG+G + +G ++N
Sbjct: 297 HAMAKGILTVHSAGNAGPNPTTVVSVAPWMLTVAATTTNRRFLTKVVLGNGKTLVGKSVN 356

Query: 337 TFDLNGTQYPLVYAGDIPNITAGFDKSMSRLCLENSVDRELVRGKIALCDKILSPKNVSS 396
            FDL G +YPL Y GD  N                     LV+GKI L  + LS   V  
Sbjct: 357 AFDLKGKKYPLEY-GDYLN-------------------ESLVKGKI-LVSRYLSGSEV-- 416

Query: 397 LKGAIGIVMQDNRSKDRTSSFPLPASHLDTQGREAISSYLTLTSLPTATILKSTE--GKT 456
              A+  +  DN  KD  S    P S L     +++ SY+  T  P  ++LK+     + 
Sbjct: 417 ---AVSFITTDN--KDYASISSRPLSVLSQDDFDSLVSYINSTRSPQGSVLKTEAIFNQL 476

Query: 457 STFVASFSSRGPNPVTLDILKPDLTGPGVEILAAWSPISPPSGAEDDNRHILFNIISGTS 516
           S  VASFSSRGPN + +DILKPD++ PGVEILAA+SP+S PS    D R + ++++SGTS
Sbjct: 477 SPKVASFSSRGPNTIAVDILKPDISAPGVEILAAYSPLSLPSEDRRDKRRVKYSVLSGTS 536

Query: 517 MSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMQAELN--KDAEFAYGSGHINPLSA 576
           M+CPH T VAAY+K+FHP WSP+ ++SA+MTTA+ M A     +  EFAYG+GH++P++A
Sbjct: 537 MACPHVTGVAAYIKTFHPDWSPSVIQSAIMTTAWQMNATGTGAESTEFAYGAGHVDPIAA 596

Query: 577 INPGLIYNATEIDYIKFLCGQGYSTKQLQQVSGDSNTCNSANKFDTVFNLNYPSFALSTT 636
           INPGL+Y   + D+I FLCG  Y++K L+ +SGD+  C+         NLNYPS +   +
Sbjct: 597 INPGLVYELNKTDHISFLCGMNYTSKTLKLISGDAVICSGKT---LQRNLNYPSMSAKLS 656

Query: 637 IS-TSFSQVYKRRVTNVGSANSTYKASIFAPLG--LNIIVNPSVLSFKALGEEKNFEVTI 696
            S +SF+  +KR VTN+G+ANSTYK+ I    G  LN+ V+PSVLS K+L E+++F VT+
Sbjct: 657 ESNSSFTVTFKRTVTNLGTANSTYKSKIVLNHGSKLNVKVSPSVLSMKSLKEKQSFTVTV 697

Query: 697 EGS-IGSSI-ASGSLVWDDGQHKVKSPIIVF 717
            GS I   + +S +L+W DG H V+SPI+V+
Sbjct: 717 SGSNIDPKLPSSANLIWSDGTHNVRSPIVVY 697

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023550545.18.8e-30681.09cucumisin-like [Cucurbita pepo subsp. pepo][more]
XP_022922167.11.5e-30279.73cucumisin-like [Cucurbita moschata][more]
KAG6579374.12.7e-29979.12hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022973015.12.6e-29778.91cucumisin-like [Cucurbita maxima][more]
XP_023551327.11.7e-29678.91cucumisin-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q395475.1e-21658.41Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9FIF81.5e-17549.40Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9FIG27.0e-17348.02Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... [more]
Q8L7D21.9e-17048.25Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... [more]
Q9FGU39.5e-17047.80Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1E2G47.5e-30379.73cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1[more]
A0A6J1IAA21.2e-29778.91cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111471532 PE=3 SV=1[more]
A0A6J1E2C51.8e-29678.37cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1[more]
A0A6J1E6I35.8e-29578.30cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430414 PE=3 SV=1[more]
A0A1S3AUT13.7e-27874.55cucumisin-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59190.11.1e-17649.40subtilase family protein [more]
AT5G59120.15.0e-17448.02subtilase 4.13 [more]
AT5G59090.11.4e-17148.25subtilase 4.12 [more]
AT5G59100.16.7e-17147.80Subtilisin-like serine endopeptidase family protein [more]
AT5G58830.13.3e-17048.19Subtilisin-like serine endopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 182..195
score: 51.49
coord: 113..132
score: 28.72
coord: 509..525
score: 48.46
NoneNo IPR availableGENE3D2.60.40.2310coord: 588..717
e-value: 7.2E-41
score: 141.0
NoneNo IPR availableGENE3D3.50.30.30coord: 313..449
e-value: 2.3E-185
score: 618.8
NoneNo IPR availablePANTHERPTHR10795:SF649PUTATIVE-RELATEDcoord: 65..716
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 96..571
score: 28.111731
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 320..444
e-value: 1.5871E-15
score: 71.6759
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 619..715
e-value: 1.0E-26
score: 93.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 115..584
e-value: 2.3E-185
score: 618.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 91..575
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 114..563
e-value: 5.4E-47
score: 160.4
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 65..716
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 510..520
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 89..545
e-value: 1.05246E-134
score: 397.739

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0015561Sed0015561gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0015561.1-exonSed0015561.1-exon-LG05:3289585..3289608exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3292219..3292300exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3293042..3293130exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3293861..3293891exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3294194..3294440exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3297469..3297982exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3298901..3299004exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3299194..3299415exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3299702..3299807exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3299913..3300126exon
Sed0015561.1-exonSed0015561.1-exon-LG05:3300860..3301482exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0015561.1-cdsSed0015561.1-cds-LG05:3289585..3289608CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3292219..3292300CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3293042..3293130CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3293861..3293891CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3294194..3294440CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3297469..3297982CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3298901..3299004CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3299194..3299415CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3299702..3299807CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3299913..3300126CDS
Sed0015561.1-cdsSed0015561.1-cds-LG05:3300860..3301401CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0015561.1-three_prime_utrSed0015561.1-three_prime_utr-LG05:3301402..3301482three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0015561.1Sed0015561.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity