Sed0000452.2 (mRNA) Chayote v1

Overview
NameSed0000452.2
TypemRNA
OrganismSechium edule (Chayote v1)
DescriptionRING-type E3 ubiquitin transferase
LocationLG05: 9965314 .. 9968114 (-)
Sequence length1330
RNA-Seq ExpressionSed0000452.2
SyntenySed0000452.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCCAGCTCTTGTTCATCGTCAAGGAGAATTTTATACAATAAGAATTTGGAAACGTTGCGTGCAGGCAATGTCAGCTTCTTGACCAATGCAAAAACCCCCCATAACTGTGTCACTGACAAAGCAATCCATCGCATTCAACATTTATGCAGTTATTGGATTGTTGAGCGGCCTAAATTTGAATATTCAGCCATTTAGGGTTTACAATCTTTTCTGCCCACTGCGTTTCTTTGCCCATTGTTCCCATCTGGGTATGAATCTTTCTTTGCCTGCTTGTGGTTTTGTTCCCACTGTTGTTTGATTCTTCATAATTCGAGTTCATTTCTGTTGGGTTTTGTTTAATTTCGTAGTTTTCGTATGTTTTTGACATTGTTTATTGGTGTGATGCTTTCTGGTTCTGTGAAACCATTTGAAACCATGGTGAATCTGTCTGGCTTCTCTCAATTCTGTTTTACACAGGGTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTGAAATTGGGAAATCGGAACTTTGCTCCAGTATTCCCGAGGAAATAACGATCATCCCTAGGCCAGACATCGAGAAATATTGAAAGAATAGAGCACGGAAGTATCTATAAATTAATGTCATATGTGAGTCTCGAACATGGGTACTAGGATTTTGAGGGAACACACTTTCAAACCCCCCAAGACTTCGACCATGGCACAACCCCTTGGGGACACAGTTATGAGGTAGATTTAATTTAAAACTAGTACTTTTGATGGACAAATTGTTGTTTTCTTTTAGCTTCCTTGTATTGTACTTTTCTATTCATAGATTCTTGTCCCAACACAGATGTCTAACTCAAAAAAGAGCATGAGAAAAGAAAAAAAAACAATGGTGCTTCTGTTGGGTTTTGTTTTGTTGCTTGTTGTACTTGTGCCAATAAGTGATGCAACTTTTATTTCCAACTCCTAAACGCACTTTTCTGTTGTACAAGCAAACAATGATTCTTCTATCCAATTATGGCTGCCATGCTGTAAATTTTCATTCTAATATGGATAAGTATTTAAAAGAGCCTTGAAGATTCTTCCTCATAGTTTCTATTTCCATCAGGTTCTGTCCTAATCCTGCTATTATAAGATATACCTTGCTTTCATGGTGTATTTATGGTAGCATTCTATTTGGTTAGGTATGTAAGAACCATATGCATTATAGATTAATTGTTCTTGATATGTATTTCCAGAGATTTATGTCTTGAATCTTCTTTTTATTCTTACAATTTGCATTGCACATTTGAAAATAAATGTCACTCCACACATTGCTCGATTTGATCTTCCTACAAAATTGATTGGTTCCCGAAACCAGTTTTGTAACTCTTTTGAGATGCTGAATAACATAGTTTCAATCTGGCAATCACACTTCTTGTTTATCTCTCAGAGATGATTGCATGAATACGGTTGGATTCAAAGAGTAAGGGATGTATCAGCTGCAGAAGTCCAGGGAAGAACATACAACTTCTGAAGCCAGCTTTTGCAAAGAAGTGACTGAGTTGCGCCTTGCGTCGATAGATTCTCAAGCAGCAACGTCTCGTGCAGAAATCAAAGGTGCCTCTTTTAGGAAAGCTTCGATGGGTGGTTTTGACAGGAATCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGGTTGTTCTAATTGTGATGAGCTTCAAAACTTACAATGCTTGCCCATAAATTATTCAGGAAAAACACTGCCCTTGATAAATCCATCCAATGAGGAAAGCATGTAATTTGAGACCCTGTTGAGAAAATGGAAGGCTACTTTGTTTAATCATTGTATTGAATTGTGGTTGAGTTATTAAATTTTAGGAAACACTAATGAAAACCAGCTAATATAGTTTTATGTTTCAGTGTTCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGTTAGGGTTCAAAACTGCTGTCCTACATGCCGCCATGAACTCGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGCGTATATGAAAACCAAGGTTGCATTGAAATGTTTCCATACAACAGCAAGCTTAAACATGAGAAGAACTGTAAATACCGCTCGTTCAAGTGCCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCCTACCTTGTCATGCATCTCAAGGACGATCACGAGGTCGATATGCACGATGGATATTCCTTTAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTAAGATCTCTTTTTGTCTATCTGTGTATGGATGGAGTTCTTTGGAAGACAATGCAACGTCCTCTGATTTCCATTCATTCCAATGCAGGTTTTTAACTGCTTTGGAAGGCAATTCTGCTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCTGTTTACATGGCGTTCCTACGGTTCATGGGTGACGATGATGATGCAAAGCAGTTTTCTTATAGTCTAGAAGTTGGTGGAAATGGCAGAAAGCTCATATGGCAAGGTATACCGAGAAGTATCCGCGATAGCCATCAGAAGGTTCGGGACAGTCACGATGGATTGATCATTCAGAGGAAGATGGCACAATTCTGCTCTGGAAATGAGGAGCAGGAACTCAAGCTAAAGGTTACAGGGCGGATATGGAGAGTGAAGTGAGAGCAAATATATATTCTCAAAGTCAGTTGTACAATATGCGACTGTAATTGGCTGCTACAATTCAGCTATGTTAGAAGTAGATATTACATCCCC

mRNA sequence

TGCCAGCTCTTGTTCATCGTCAAGGAGAATTTTATACAATAAGAATTTGGAAACGTTGCGTGCAGGCAATGTCAGCTTCTTGACCAATGCAAAAACCCCCCATAACTGTGTCACTGACAAAGCAATCCATCGCATTCAACATTTATGCAGTTATTGGATTGTTGAGCGGCCTAAATTTGAATATTCAGCCATTTAGGGTTTACAATCTTTTCTGCCCACTGCGTTTCTTTGCCCATTGTTCCCATCTGGAGATGATTGCATGAATACGGTTGGATTCAAAGAGTAAGGGATGTATCAGCTGCAGAAGTCCAGGGAAGAACATACAACTTCTGAAGCCAGCTTTTGCAAAGAAGTGACTGAGTTGCGCCTTGCGTCGATAGATTCTCAAGCAGCAACGTCTCGTGCAGAAATCAAAGGTGCCTCTTTTAGGAAAGCTTCGATGGGTGGTTTTGACAGGAATCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTTCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGTTAGGGTTCAAAACTGCTGTCCTACATGCCGCCATGAACTCGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGCGTATATGAAAACCAAGGTTGCATTGAAATGTTTCCATACAACAGCAAGCTTAAACATGAGAAGAACTGTAAATACCGCTCGTTCAAGTGCCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCCTACCTTGTCATGCATCTCAAGGACGATCACGAGGTCGATATGCACGATGGATATTCCTTTAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTTTTTAACTGCTTTGGAAGGCAATTCTGCTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCTGTTTACATGGCGTTCCTACGGTTCATGGGTGACGATGATGATGCAAAGCAGTTTTCTTATAGTCTAGAAGTTGGTGGAAATGGCAGAAAGCTCATATGGCAAGGTATACCGAGAAGTATCCGCGATAGCCATCAGAAGGTTCGGGACAGTCACGATGGATTGATCATTCAGAGGAAGATGGCACAATTCTGCTCTGGAAATGAGGAGCAGGAACTCAAGCTAAAGGTTACAGGGCGGATATGGAGAGTGAAGTGAGAGCAAATATATATTCTCAAAGTCAGTTGTACAATATGCGACTGTAATTGGCTGCTACAATTCAGCTATGTTAGAAGTAGATATTACATCCCC

Coding sequence (CDS)

ATGTATCAGCTGCAGAAGTCCAGGGAAGAACATACAACTTCTGAAGCCAGCTTTTGCAAAGAAGTGACTGAGTTGCGCCTTGCGTCGATAGATTCTCAAGCAGCAACGTCTCGTGCAGAAATCAAAGGTGCCTCTTTTAGGAAAGCTTCGATGGGTGGTTTTGACAGGAATCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTTCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGTTAGGGTTCAAAACTGCTGTCCTACATGCCGCCATGAACTCGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGCGTATATGAAAACCAAGGTTGCATTGAAATGTTTCCATACAACAGCAAGCTTAAACATGAGAAGAACTGTAAATACCGCTCGTTCAAGTGCCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCCTACCTTGTCATGCATCTCAAGGACGATCACGAGGTCGATATGCACGATGGATATTCCTTTAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTTTTTAACTGCTTTGGAAGGCAATTCTGCTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCTGTTTACATGGCGTTCCTACGGTTCATGGGTGACGATGATGATGCAAAGCAGTTTTCTTATAGTCTAGAAGTTGGTGGAAATGGCAGAAAGCTCATATGGCAAGGTATACCGAGAAGTATCCGCGATAGCCATCAGAAGGTTCGGGACAGTCACGATGGATTGATCATTCAGAGGAAGATGGCACAATTCTGCTCTGGAAATGAGGAGCAGGAACTCAAGCTAAAGGTTACAGGGCGGATATGGAGAGTGAAGTGA

Protein sequence

MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRNDVHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAESLELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEEQELKLKVTGRIWRVK
Homology
BLAST of Sed0000452.2 vs. NCBI nr
Match: XP_038900383.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 609.0 bits (1569), Expect = 2.3e-170
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRND 60
           MYQ QKSREEH  S ASFCKEVTELRL SIDSQAATSR E KG +FRKAS GGF R+RND
Sbjct: 23  MYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKASTGGFARSRND 82

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           VHELLDCTVCMNLMHPPIYQC+NGHTLCSSCK R+QNCCPTCRHELGNIRCLALEKVAES
Sbjct: 83  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIRCLALEKVAES 142

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPCVY+N GCI+MFPY SKLKHEKNCKYR + CPYAGGEC VTGDIPYLVMHLKDDHE
Sbjct: 143 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPYLVMHLKDDHE 202

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMHDG SFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 203 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 262

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSH+KVRDSHDGLIIQRK+A FCSGNE 
Sbjct: 263 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 322

Query: 301 QELKLKVTGRIWRV 315
            ELKLKVTGRIWRV
Sbjct: 323 MELKLKVTGRIWRV 336

BLAST of Sed0000452.2 vs. NCBI nr
Match: XP_038900384.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_038900386.1 E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida])

HSP 1 Score: 609.0 bits (1569), Expect = 2.3e-170
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRND 60
           MYQ QKSREEH  S ASFCKEVTELRL SIDSQAATSR E KG +FRKAS GGF R+RND
Sbjct: 11  MYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKASTGGFARSRND 70

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           VHELLDCTVCMNLMHPPIYQC+NGHTLCSSCK R+QNCCPTCRHELGNIRCLALEKVAES
Sbjct: 71  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIRCLALEKVAES 130

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPCVY+N GCI+MFPY SKLKHEKNCKYR + CPYAGGEC VTGDIPYLVMHLKDDHE
Sbjct: 131 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPYLVMHLKDDHE 190

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMHDG SFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 191 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 250

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSH+KVRDSHDGLIIQRK+A FCSGNE 
Sbjct: 251 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 310

Query: 301 QELKLKVTGRIWRV 315
            ELKLKVTGRIWRV
Sbjct: 311 MELKLKVTGRIWRV 324

BLAST of Sed0000452.2 vs. NCBI nr
Match: XP_038900385.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X4 [Benincasa hispida])

HSP 1 Score: 609.0 bits (1569), Expect = 2.3e-170
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRND 60
           MYQ QKSREEH  S ASFCKEVTELRL SIDSQAATSR E KG +FRKAS GGF R+RND
Sbjct: 1   MYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKASTGGFARSRND 60

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           VHELLDCTVCMNLMHPPIYQC+NGHTLCSSCK R+QNCCPTCRHELGNIRCLALEKVAES
Sbjct: 61  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIRCLALEKVAES 120

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPCVY+N GCI+MFPY SKLKHEKNCKYR + CPYAGGEC VTGDIPYLVMHLKDDHE
Sbjct: 121 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPYLVMHLKDDHE 180

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMHDG SFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 181 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSH+KVRDSHDGLIIQRK+A FCSGNE 
Sbjct: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 300

Query: 301 QELKLKVTGRIWRV 315
            ELKLKVTGRIWRV
Sbjct: 301 MELKLKVTGRIWRV 314

BLAST of Sed0000452.2 vs. NCBI nr
Match: XP_038900382.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 609.0 bits (1569), Expect = 2.3e-170
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRND 60
           MYQ QKSREEH  S ASFCKEVTELRL SIDSQAATSR E KG +FRKAS GGF R+RND
Sbjct: 23  MYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKASTGGFARSRND 82

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           VHELLDCTVCMNLMHPPIYQC+NGHTLCSSCK R+QNCCPTCRHELGNIRCLALEKVAES
Sbjct: 83  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNIRCLALEKVAES 142

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPCVY+N GCI+MFPY SKLKHEKNCKYR + CPYAGGEC VTGDIPYLVMHLKDDHE
Sbjct: 143 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIPYLVMHLKDDHE 202

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMHDG SFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 203 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 262

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSH+KVRDSHDGLIIQRK+A FCSGNE 
Sbjct: 263 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 322

Query: 301 QELKLKVTGRIWRV 315
            ELKLKVTGRIWRV
Sbjct: 323 MELKLKVTGRIWRV 336

BLAST of Sed0000452.2 vs. NCBI nr
Match: XP_008449780.1 (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo])

HSP 1 Score: 600.9 bits (1548), Expect = 6.3e-168
Identity = 281/315 (89.21%), Postives = 293/315 (93.02%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRND 60
           MYQ QKSREEH +S ASFCKEVTEL L SIDSQ ATSR E KG +FRKAS GGF R+RND
Sbjct: 12  MYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKASTGGFARSRND 71

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           VHELLDCTVCMNLMHPPIYQC+NGHTLCSSCK RVQNCCPTCRHELGNIRCLALEKVAES
Sbjct: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPCVY+N GCI+MFPY SKLKHEKNCKYR + CPYAGGEC VTGDIP LVMHLKDDHE
Sbjct: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMHDG SFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GDD++AKQFSYSLEVGGNGRKLIWQGIPRSIRDSH+KVRDSHDGLIIQRK+A FCSGNE 
Sbjct: 252 GDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311

Query: 301 QELKLKVTGRIWRVK 316
            ELKLKVTGRIWRV+
Sbjct: 312 MELKLKVTGRIWRVR 326

BLAST of Sed0000452.2 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 4.8e-126
Identity = 208/301 (69.10%), Postives = 247/301 (82.06%), Query Frame = 0

Query: 17  SFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDR----NRNDVHELLDCTVCMN 76
           S  KEV E     +D +  T++ E+      K    G  +    + N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 77  LMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAESLELPCVYENQGC 136
           LM+PPI+QC NGHTLCS+CK+RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+N GC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 137 IEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGYSFSHR 196
            ++FPY SKLKHE++C++R + CPYAG ECSVTGDIP LV+HLKDDH+VDMHDG +F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 197 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYS 256
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 257 LEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEEQELKLKVTGRIW 314
           LEVG +GRKL WQGIPRSIRDSH+KVRDS DGLII R +A + SG + QELKL+VTGRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

BLAST of Sed0000452.2 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 2.0e-124
Identity = 208/299 (69.57%), Postives = 247/299 (82.61%), Query Frame = 0

Query: 17  SFCKEVTELRLASIDSQAATSRAEI--KGASFRKASMGGFDRNRNDVHELLDCTVCMNLM 76
           S  KE  E     +D +   ++ E   K       S+G F  + N V+ELL+C VC NLM
Sbjct: 6   SALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKF-HSSNGVYELLECPVCTNLM 65

Query: 77  HPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAESLELPCVYENQGCIE 136
           +PPI+QC NGHTLCSSCK+RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+N GC +
Sbjct: 66  YPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQD 125

Query: 137 MFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGYSFSHRYV 196
           +FPY SKLKHE++C++RS+ CPYAG ECSVTGDIP LV HLKDDH+VDMHDG +F+HRYV
Sbjct: 126 IFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYV 185

Query: 197 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLE 256
           KSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYSLE
Sbjct: 186 KSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLE 245

Query: 257 VGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEEQELKLKVTGRIWR 314
           VG + RKL WQGIPRSIRDSH+KVRDS DGLII R +A + SG++++ELKL+VTGRIW+
Sbjct: 246 VGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

BLAST of Sed0000452.2 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 3.9e-120
Identity = 192/255 (75.29%), Postives = 222/255 (87.06%), Query Frame = 0

Query: 59  NDVHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVA 118
           + V ELL+C VC++ M+PPI+QCSNGHTLCS CK RV N CPTCRHELGNIRCLALEKVA
Sbjct: 45  SSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 104

Query: 119 ESLELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDD 178
            SLELPC Y+N GC+ ++PY  KLKHE  C+YR + CPYAG EC+V GDIPYLV HLKDD
Sbjct: 105 ASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDD 164

Query: 179 HEVDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLR 238
           H+VDMH+G +F+HRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAF+LG APVY+AFLR
Sbjct: 165 HKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 224

Query: 239 FMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGN 298
           FMGDD +AK +SYSLEVGG GRK+IWQG+PRSIRDSH+KVRDS+DGLIIQR MA F SG 
Sbjct: 225 FMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGG 284

Query: 299 EEQELKLKVTGRIWR 314
           E +ELKL+VTGRIW+
Sbjct: 285 ERKELKLRVTGRIWK 299

BLAST of Sed0000452.2 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 3.7e-118
Identity = 191/265 (72.08%), Postives = 223/265 (84.15%), Query Frame = 0

Query: 49  ASMGGFDRNRNDVHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGN 108
           A+  G       VHELL+C VC N M+PPI+QC NGHTLCS+CK RV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 109 IRCLALEKVAESLELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDI 168
           IRCLALEKVAESLELPC + + GC E+FPY SKLKHE  C +R + CPYAG ECSVTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 169 PYLVMHLKDDHEVDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 228
           P+LV HL+DDH+VDMH G +F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 229 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQ 288
            APVYMAFLRFMGD+ +A+ ++YSLEVGG GRKLIW+G PRS+RDSH+KVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 289 RKMAQFCSGNEEQELKLKVTGRIWR 314
           R MA F SG + +ELKL+VTGRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

BLAST of Sed0000452.2 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 419.5 bits (1077), Expect = 3.4e-116
Identity = 185/253 (73.12%), Postives = 217/253 (85.77%), Query Frame = 0

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           V+ELL+C VC N M+PPI+QC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 40  VYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAES 99

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPC Y N GC+ +FPY SKLKHE  C +R + CPYAG EC+  GDI +LV HL+DDH+
Sbjct: 100 LELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHK 159

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMH G +F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG APVYMAFLRFM
Sbjct: 160 VDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFM 219

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GD+DDA+ ++YSLEVGG+GRK  W+G PRS+RDSH+KVRDSHDGLIIQR MA F SG ++
Sbjct: 220 GDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDK 279

Query: 301 QELKLKVTGRIWR 314
           +ELKL+VTGRIW+
Sbjct: 280 KELKLRVTGRIWK 292

BLAST of Sed0000452.2 vs. ExPASy TrEMBL
Match: A0A5D3DDZ5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00330 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.1e-168
Identity = 281/315 (89.21%), Postives = 293/315 (93.02%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRND 60
           MYQ QKSREEH +S ASFCKEVTEL L SIDSQ ATSR E KG +FRKAS GGF R+RND
Sbjct: 1   MYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKASTGGFARSRND 60

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           VHELLDCTVCMNLMHPPIYQC+NGHTLCSSCK RVQNCCPTCRHELGNIRCLALEKVAES
Sbjct: 61  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 120

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPCVY+N GCI+MFPY SKLKHEKNCKYR + CPYAGGEC VTGDIP LVMHLKDDHE
Sbjct: 121 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 180

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMHDG SFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 181 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GDD++AKQFSYSLEVGGNGRKLIWQGIPRSIRDSH+KVRDSHDGLIIQRK+A FCSGNE 
Sbjct: 241 GDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 300

Query: 301 QELKLKVTGRIWRVK 316
            ELKLKVTGRIWRV+
Sbjct: 301 MELKLKVTGRIWRVR 315

BLAST of Sed0000452.2 vs. ExPASy TrEMBL
Match: A0A1S3BMT4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.1e-168
Identity = 281/315 (89.21%), Postives = 293/315 (93.02%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRND 60
           MYQ QKSREEH +S ASFCKEVTEL L SIDSQ ATSR E KG +FRKAS GGF R+RND
Sbjct: 12  MYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKASTGGFARSRND 71

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           VHELLDCTVCMNLMHPPIYQC+NGHTLCSSCK RVQNCCPTCRHELGNIRCLALEKVAES
Sbjct: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPCVY+N GCI+MFPY SKLKHEKNCKYR + CPYAGGEC VTGDIP LVMHLKDDHE
Sbjct: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMHDG SFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GDD++AKQFSYSLEVGGNGRKLIWQGIPRSIRDSH+KVRDSHDGLIIQRK+A FCSGNE 
Sbjct: 252 GDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311

Query: 301 QELKLKVTGRIWRVK 316
            ELKLKVTGRIWRV+
Sbjct: 312 MELKLKVTGRIWRVR 326

BLAST of Sed0000452.2 vs. ExPASy TrEMBL
Match: A0A0A0KWV9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.2e-168
Identity = 281/315 (89.21%), Postives = 292/315 (92.70%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSEASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRND 60
           MYQ  KSREEH +S ASFCKEVTELRL SIDSQ ATSR E KG +FRKAS GGF R+RND
Sbjct: 12  MYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGFARSRND 71

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           VHELLDCTVCMNLMHPPIYQC+NGHTLCSSCK RVQNCCPTCRHELGNIRCLALEKVAES
Sbjct: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPCVY+N GCI+MFPY SKLKHEKNCKYR + CPYAGGEC VTGDIP LVMHLKDDHE
Sbjct: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMHDG SFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM
Sbjct: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GDD +AKQFSYSLEVGGNGRKLIWQGIPRSIRDSH+KVRDSHDGLIIQRK+A FCSGNE 
Sbjct: 252 GDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311

Query: 301 QELKLKVTGRIWRVK 316
            ELKLKVTGRIWRV+
Sbjct: 312 MELKLKVTGRIWRVR 326

BLAST of Sed0000452.2 vs. ExPASy TrEMBL
Match: A0A6J1DI06 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021266 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.9e-157
Identity = 268/316 (84.81%), Postives = 284/316 (89.87%), Query Frame = 0

Query: 1   MYQLQKSREEHTTSE-ASFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDRNRN 60
           MYQ +K REEHT+S  ASFCKEVTELRL SIDSQAATSR E K  S RK+S  GF RN N
Sbjct: 11  MYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSST-GFGRNCN 70

Query: 61  DVHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAE 120
           +VHELLDCTVCM+LMHPPIYQC+NGHTLCSSCK RV NCCPTCRHELGNIRCL LEKVAE
Sbjct: 71  NVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAE 130

Query: 121 SLELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDH 180
           SLELPCVY+N GCI+  PY SKLKHEKNCKYR + CPYAGGECSVTGDIPYLVMHLKDDH
Sbjct: 131 SLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDH 190

Query: 181 EVDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF 240
           EVDMH+G SFSHRYVKSNP+EVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF
Sbjct: 191 EVDMHEGCSFSHRYVKSNPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF 250

Query: 241 MGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNE 300
           MGDDDDAK+FSYSLEVGGNGRKL+WQG PRSIRDSH+KVRDS DGLII R +A FCSGN 
Sbjct: 251 MGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNH 310

Query: 301 EQELKLKVTGRIWRVK 316
            QELKLKV GRIWRV+
Sbjct: 311 GQELKLKVVGRIWRVR 325

BLAST of Sed0000452.2 vs. ExPASy TrEMBL
Match: A0A5C7IUW2 (RING-type E3 ubiquitin transferase OS=Acer yangbiense OX=1000413 GN=EZV62_001706 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.0e-134
Identity = 224/297 (75.42%), Postives = 257/297 (86.53%), Query Frame = 0

Query: 19  CKEVTELRLASIDSQAATSRAEIKGASFRKAS--MGGFDRNRNDVHELLDCTVCMNLMHP 78
           CKE+ E R+A  D +  TS  E++G+  RKA+   GG   + NDVHELL+C VCMNLM+P
Sbjct: 8   CKEIIESRIAFADYETTTSNVELRGSPCRKATTGFGGNTTSTNDVHELLECPVCMNLMYP 67

Query: 79  PIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAESLELPCVYENQGCIEMF 138
           PIYQC NGHT+CS+CKVRV+N CPTCRHELGNIRCLALEKVAESLELPC Y+  GC ++F
Sbjct: 68  PIYQCPNGHTVCSNCKVRVRNSCPTCRHELGNIRCLALEKVAESLELPCRYQILGCHDIF 127

Query: 139 PYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGYSFSHRYVKS 198
           PY  KLKHEKNCKYR + CPYAG ECSVTGDIP+LVMHLK+DH+VDMHDG +F+HRYVK+
Sbjct: 128 PYYGKLKHEKNCKYRPYNCPYAGAECSVTGDIPFLVMHLKNDHKVDMHDGSTFNHRYVKA 187

Query: 199 NPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLEVG 258
           N QEVENATWMLTVFNCFGRQFCLHFEAF LG+APVYMAFLRFMGD+D+A+QFSY+LEVG
Sbjct: 188 NAQEVENATWMLTVFNCFGRQFCLHFEAFHLGSAPVYMAFLRFMGDEDEARQFSYTLEVG 247

Query: 259 GNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEEQELKLKVTGRIWR 314
           GNGRKL WQGIPRSIRDSH+KVRDS DGLIIQR +A F SG ++QELKLKV GRIW+
Sbjct: 248 GNGRKLTWQGIPRSIRDSHRKVRDSQDGLIIQRNLALFFSGGDKQELKLKVAGRIWK 304

BLAST of Sed0000452.2 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 452.2 bits (1162), Expect = 3.4e-127
Identity = 208/301 (69.10%), Postives = 247/301 (82.06%), Query Frame = 0

Query: 17  SFCKEVTELRLASIDSQAATSRAEIKGASFRKASMGGFDR----NRNDVHELLDCTVCMN 76
           S  KEV E     +D +  T++ E+      K    G  +    + N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 77  LMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAESLELPCVYENQGC 136
           LM+PPI+QC NGHTLCS+CK+RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+N GC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 137 IEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGYSFSHR 196
            ++FPY SKLKHE++C++R + CPYAG ECSVTGDIP LV+HLKDDH+VDMHDG +F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 197 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYS 256
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 257 LEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEEQELKLKVTGRIW 314
           LEVG +GRKL WQGIPRSIRDSH+KVRDS DGLII R +A + SG + QELKL+VTGRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

BLAST of Sed0000452.2 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 446.8 bits (1148), Expect = 1.4e-125
Identity = 208/299 (69.57%), Postives = 247/299 (82.61%), Query Frame = 0

Query: 17  SFCKEVTELRLASIDSQAATSRAEI--KGASFRKASMGGFDRNRNDVHELLDCTVCMNLM 76
           S  KE  E     +D +   ++ E   K       S+G F  + N V+ELL+C VC NLM
Sbjct: 6   SALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKF-HSSNGVYELLECPVCTNLM 65

Query: 77  HPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAESLELPCVYENQGCIE 136
           +PPI+QC NGHTLCSSCK+RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+N GC +
Sbjct: 66  YPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQD 125

Query: 137 MFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGYSFSHRYV 196
           +FPY SKLKHE++C++RS+ CPYAG ECSVTGDIP LV HLKDDH+VDMHDG +F+HRYV
Sbjct: 126 IFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYV 185

Query: 197 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLE 256
           KSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYSLE
Sbjct: 186 KSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLE 245

Query: 257 VGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEEQELKLKVTGRIWR 314
           VG + RKL WQGIPRSIRDSH+KVRDS DGLII R +A + SG++++ELKL+VTGRIW+
Sbjct: 246 VGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

BLAST of Sed0000452.2 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 426.0 bits (1094), Expect = 2.6e-119
Identity = 191/265 (72.08%), Postives = 223/265 (84.15%), Query Frame = 0

Query: 49  ASMGGFDRNRNDVHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGN 108
           A+  G       VHELL+C VC N M+PPI+QC NGHTLCS+CK RV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 109 IRCLALEKVAESLELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDI 168
           IRCLALEKVAESLELPC + + GC E+FPY SKLKHE  C +R + CPYAG ECSVTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 169 PYLVMHLKDDHEVDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 228
           P+LV HL+DDH+VDMH G +F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 229 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQ 288
            APVYMAFLRFMGD+ +A+ ++YSLEVGG GRKLIW+G PRS+RDSH+KVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 289 RKMAQFCSGNEEQELKLKVTGRIWR 314
           R MA F SG + +ELKL+VTGRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

BLAST of Sed0000452.2 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 417.2 bits (1071), Expect = 1.2e-116
Identity = 185/253 (73.12%), Postives = 218/253 (86.17%), Query Frame = 0

Query: 61  VHELLDCTVCMNLMHPPIYQCSNGHTLCSSCKVRVQNCCPTCRHELGNIRCLALEKVAES 120
           V+ELL+C VC   M+PPI+QC NGHTLCS+CKVRV N CPTCR ELG+IRCLALEKVAES
Sbjct: 58  VYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAES 117

Query: 121 LELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYLVMHLKDDHE 180
           LELPC + N GC E+FPY SKLKHE  C +R + CPYAG EC + GDIP+LV HL+DDH+
Sbjct: 118 LELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHK 177

Query: 181 VDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 240
           VDMH G +F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG  PVYMAFLRFM
Sbjct: 178 VDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFM 237

Query: 241 GDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKMAQFCSGNEE 300
           GD++DA+ +SYSLEVGG+GRKL W+G PRSIRDSH+KVRDS+DGLIIQR MA F SG + 
Sbjct: 238 GDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDR 297

Query: 301 QELKLKVTGRIWR 314
           +ELKL+VTG+IW+
Sbjct: 298 KELKLRVTGKIWK 310

BLAST of Sed0000452.2 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.8e-91
Identity = 147/202 (72.77%), Postives = 174/202 (86.14%), Query Frame = 0

Query: 112 LALEKVAESLELPCVYENQGCIEMFPYNSKLKHEKNCKYRSFKCPYAGGECSVTGDIPYL 171
           +ALEKVAESLELPC Y N GC+ +FPY SKLKHE  C +R + CPYAG EC+  GDI +L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 172 VMHLKDDHEVDMHDGYSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 231
           V HL+DDH+VDMH G +F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG AP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 232 VYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHQKVRDSHDGLIIQRKM 291
           VYMAFLRFMGD+DDA+ ++YSLEVGG+GRK  W+G PRS+RDSH+KVRDSHDGLIIQR M
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 292 AQFCSGNEEQELKLKVTGRIWR 314
           A F SG +++ELKL+VTGRIW+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWK 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900383.12.3e-17090.76E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida][more]
XP_038900384.12.3e-17090.76E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_03890... [more]
XP_038900385.12.3e-17090.76E3 ubiquitin-protein ligase SINAT2-like isoform X4 [Benincasa hispida][more]
XP_038900382.12.3e-17090.76E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida][more]
XP_008449780.16.3e-16889.21PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9M2P44.8e-12669.10E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
P937482.0e-12469.57Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S... [more]
Q10L913.9e-12075.29E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q84JL33.7e-11872.08E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q8S3N13.4e-11673.12E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Match NameE-valueIdentityDescription
A0A5D3DDZ53.1e-16889.21RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BMT43.1e-16889.21RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 ... [more]
A0A0A0KWV95.2e-16889.21RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE... [more]
A0A6J1DI061.9e-15784.81RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102126... [more]
A0A5C7IUW21.0e-13475.42RING-type E3 ubiquitin transferase OS=Acer yangbiense OX=1000413 GN=EZV62_001706... [more]
Match NameE-valueIdentityDescription
AT3G58040.13.4e-12769.10seven in absentia of Arabidopsis 2 [more]
AT2G41980.11.4e-12569.57Protein with RING/U-box and TRAF-like domains [more]
AT3G61790.12.6e-11972.08Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.11.2e-11673.12Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.11.8e-9172.77TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 8..119
e-value: 5.9E-7
score: 31.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 120..174
e-value: 6.6E-16
score: 60.3
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 175..308
e-value: 2.2E-37
score: 129.8
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 109..308
e-value: 1.7E-78
score: 263.2
NoneNo IPR availablePANTHERPTHR10315:SF123E3 UBIQUITIN-PROTEIN LIGASE SINAT2-LIKEcoord: 17..313
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 17..313
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 65..103
e-value: 1.07138E-13
score: 62.3022
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 120..312
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 50..107
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 120..180
score: 13.153067
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 67..103
score: 9.258371

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0000452Sed0000452gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0000452.2-three_prime_utrSed0000452.2-three_prime_utr-LG05:9965314..9965406three_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0000452.2-exonSed0000452.2-exon-LG05:9965314..9965727exon
Sed0000452.2-exonSed0000452.2-exon-LG05:9965816..9966202exon
Sed0000452.2-exonSed0000452.2-exon-LG05:9966428..9966707exon
Sed0000452.2-exonSed0000452.2-exon-LG05:9967866..9968114exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0000452.2-cdsSed0000452.2-cds-LG05:9965407..9965727CDS
Sed0000452.2-cdsSed0000452.2-cds-LG05:9965816..9966202CDS
Sed0000452.2-cdsSed0000452.2-cds-LG05:9966428..9966667CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0000452.2-five_prime_utrSed0000452.2-five_prime_utr-LG05:9966668..9966707five_prime_UTR
Sed0000452.2-five_prime_utrSed0000452.2-five_prime_utr-LG05:9967866..9968114five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0000452.2Sed0000452.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016740 transferase activity
molecular_function GO:0061630 ubiquitin protein ligase activity