PI0008078.1 (mRNA) Melon (PI 482460) v1

Overview
NamePI0008078.1
TypemRNA
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionDDE Tnp4 domain-containing protein
Locationchr12: 23059177 .. 23062306 (-)
Sequence length1737
RNA-Seq ExpressionPI0008078.1
SyntenyPI0008078.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCGCCGTCAGATTTGTCTCACAAAACAAAATCGCCTTTAGCTCATTCGCTAAGCCAAAACTTCTTGCTCTCTCTCATGGACTCCACCTTTTGTTCTTAGACTAATTTAAATTTTGTTTCATTCAAAAGGGCGGCCCGATCTTTCTCCATTGCATGTGCTACAAAACCCAATTCCTGTTTTTACCAAATTTGAAGAATCTGTGTGCGATTTTGTGGAATATTATGTGTCCTCTTCATCAATAACATTTCTTGATTTTTGGGTTTTAATCACTCACTCGTTGTGGCTAATGGGTCCAATCAGAGGGTTGAGAAAGAAGAAGAAATTAGAGAGAAAGATCGATTCCAATGGTACTGCTTCTGATTCTTCTGAAAAGGATGAGGCTATAGATTGGTGGGATGATTTCTCCAAACGAACCAATGGTATTTCTATCTTTTTCTTTTTAATGTCTTGATTTTCTCTTTACTTTTAATCGAAGTTCTTCAATCGTAAGATCGGTTCTGTTTGACTACTGACTGTTTTGGAACAAATGATTTGAACTTGGTTGACTTCTTATCTGGTGTGTAGAAAGAATTAACGGTACTGCATGCATTGGGAGTCTTGGTAATTATGTTAGCTTCGAGTTATTGTATAGAAGGGCAAGAACTTTCGTTGGGTTTCTATAACGAAATATGTACGTTATGTGATTCTGTTTATAAGAGTTTGAATGAGTCAATGTTGTGGCCATGTTTCTGAGATTTTGATCTGTTTTCTGTGCTTGTAATGTTTAGAGTTCGACAGGGGATCTCGTTTTGGGTTTGATGTGTTAGTGTTCAGTTTGTGAAGGGATAGATTATGCTGTTTGATTGTTCTAACTTATGACATTACACCTTCATAAGCTGTATTTAGTATTCTTTGCTTATCGAGAGCTTCTCGAATTTGTAACTAGTTGATCCTGATTCGACATTAGTAGTTTATTGATGATAGTGACGAAATAGACCCAAAGCTGTGGAACTTGAGTGTAAGTCTCTTGTTGAAATGGAATGGCCTTATCTTTATTTTCATCTCTCCCCAAATTTGAGTGCTAGTTTTTTGAATTACAAGGATGCTTCTATATACTAGGGTGGGGTGGTAATATATTCTTATGTGTGTTCCTTCTTCCAACCTTTTCGCTAGTTATTGGCTGTCCTTCCTTCTTCAGGATGTATGACAATGTCCTCCGCCAACGACTACCCTTGAAAGTATGGTTCTGTTATGAGTGAAGTCCAGAATCAACAAGAATGACACCCATCTTGTTTAGGAATTCCTTCCTATTATCTTAGGGAATGTAGAGAATTTATGCTATTTCTCTATCATATCTGATTCTAGCGATTAGATAATATAACTTCCATATGTTACTAAATTGTGTTTTTAAGTGTTGAGACAACTATAGAAGACTAAGATAAACTCGTTGGACTGATACAGAGTCGTCAGAATGGATTGGAGAGTTAGAAACAAAGATGTGGATTGTTAATGGAATGTAGTTATTCGATTAATAGGCTATATAAATCACATCATACGATTTACATCCTCATTGCTTTGGCATTGACGCAATGTCATTAGATTTTTTCTTCTGGTGTTTATCAGTTTCTCAAGTCTTCTACTTTACATATTTGAGAGCCAGTGAGTATCTTTTTCCATTACACTACAAAGTGAAAAACTAATCATACTGGTCTTGAATGTATCCAATGATTTGGGGATTAGCTGAGCCTTTTCTCCTCTAAATATTCTGGACCAAATTTCAGTGTTTTAGTTTGAAATTTTTGGTTACACTTTCTTGGCCCCATTTCCATGCAGGTCTTCATTCTGCATCAAAAGGGTTGGATAGATTCAAATCCATTTTCAAGGTCTCCCGAAAGACTTTTGATTACATATGTTTGCTTGTCAAGGACGATATGACAGCTAAATCTGGTCATTTTACATTTTTGAACGGTAGGCCGTTGTCTTTATGTGATCAAGTTGCTGTAGCTTTAAGAAGGCTGGGGTCCGGTGAATCATTAGTGACAATAGGTGATTCACTTGGATTGAACCACTCGACTGTGTCTCAAGTCACATGGCGATTTGTGGAGTCAATGGAAGAAAGGGGGCTTCGCCATCTTCATTGGCCTTCAAACGAAGTGGAAATGGCTCAAGTGAAATCAAAATTTGAGAAAATACAAGGACTTCCTAACTGTTGTGGTTCGATCGATGCCACTCACATCATGATGTGCCTGCCTGCTTCGGATCCTACAAGTTATGTGTGGCTTGATGATGAAAAAAACCACAGCATGGTTTTGCAAGTGATTGTAGATGCAGAAATGAGGTTCCGAGACATATTAACTGGATTGCCTGGAAAATTGTCGGATTGGTTAGTTTTCCAGAGTTCAAACTTCCACAAGCTTTGTGACAAGGGGGAGAGGCTGAATGGGAAGAGGTTAGAGCTTCCCGACAGATCGGAAATCCGAGAATATATAGTTGGAGATTCAGGCTATCCTTTACTTCCCTATCTTGTCACTCCCTATGATGGAAAAGAACTCTCAACATCAAAAGCCGAGTTCAACAAGCGACACAAAGAGACTCGGTTGGTGGTTCAACAAGCACTAGCAATGTTGAAAGAACGGTGGAGGATCATTCAAGGAGTGATGTGGAGACCCGATAAACATCGGTTGCCGAGAATCATTCTAGTCTGCTGCTTACTTCATAACATTATCATTGATATCGGAGACGAGACGGAGGATGGTGACATTCCTTTGTCTGTCGAACATGATGTTGATTACAAACAACAGGTATGTGATGTTTTTGACTCAAAGGGTGCATATTTGAGAGATAGATTGTCTTTGCTGTGCATATGAAACTGAGAGGCTTTGGTTACCATATTTACTCTTCTTTTACATTCTACAACGTTTAAATGATGGGATTAAGCTTCTGCCTCATGTACATATCATCAATTGATGCTTTAGTCATTTATTGGGGAGTGATTGATGAATTGATTCAGGTCCAATGATCTGAAATTTGTCTTATGAACATTGAAAGAGGGAAATTACAAAACACACAAGTTATTAGTATATGAGCTGTGGTTTTTATTCTTTTGTTGTGAACAGTATGGAATTACAGCGTTACATACAAGTTTCAAT

mRNA sequence

CTCCGCCGTCAGATTTGTCTCACAAAACAAAATCGCCTTTAGCTCATTCGCTAAGCCAAAACTTCTTGCTCTCTCTCATGGACTCCACCTTTTGTTCTTAGACTAATTTAAATTTTGTTTCATTCAAAAGGGCGGCCCGATCTTTCTCCATTGCATGTGCTACAAAACCCAATTCCTGTTTTTACCAAATTTGAAGAATCTGTGTGCGATTTTGTGGAATATTATGTGTCCTCTTCATCAATAACATTTCTTGATTTTTGGGTTTTAATCACTCACTCGTTGTGGCTAATGGGTCCAATCAGAGGGTTGAGAAAGAAGAAGAAATTAGAGAGAAAGATCGATTCCAATGGTACTGCTTCTGATTCTTCTGAAAAGGATGAGGCTATAGATTGGTGGGATGATTTCTCCAAACGAACCAATGGTCTTCATTCTGCATCAAAAGGGTTGGATAGATTCAAATCCATTTTCAAGGTCTCCCGAAAGACTTTTGATTACATATGTTTGCTTGTCAAGGACGATATGACAGCTAAATCTGGTCATTTTACATTTTTGAACGGTAGGCCGTTGTCTTTATGTGATCAAGTTGCTGTAGCTTTAAGAAGGCTGGGGTCCGGTGAATCATTAGTGACAATAGGTGATTCACTTGGATTGAACCACTCGACTGTGTCTCAAGTCACATGGCGATTTGTGGAGTCAATGGAAGAAAGGGGGCTTCGCCATCTTCATTGGCCTTCAAACGAAGTGGAAATGGCTCAAGTGAAATCAAAATTTGAGAAAATACAAGGACTTCCTAACTGTTGTGGTTCGATCGATGCCACTCACATCATGATGTGCCTGCCTGCTTCGGATCCTACAAGTTATGTGTGGCTTGATGATGAAAAAAACCACAGCATGGTTTTGCAAGTGATTGTAGATGCAGAAATGAGGTTCCGAGACATATTAACTGGATTGCCTGGAAAATTGTCGGATTGGTTAGTTTTCCAGAGTTCAAACTTCCACAAGCTTTGTGACAAGGGGGAGAGGCTGAATGGGAAGAGGTTAGAGCTTCCCGACAGATCGGAAATCCGAGAATATATAGTTGGAGATTCAGGCTATCCTTTACTTCCCTATCTTGTCACTCCCTATGATGGAAAAGAACTCTCAACATCAAAAGCCGAGTTCAACAAGCGACACAAAGAGACTCGGTTGGTGGTTCAACAAGCACTAGCAATGTTGAAAGAACGGTGGAGGATCATTCAAGGAGTGATGTGGAGACCCGATAAACATCGGTTGCCGAGAATCATTCTAGTCTGCTGCTTACTTCATAACATTATCATTGATATCGGAGACGAGACGGAGGATGGTGACATTCCTTTGTCTGTCGAACATGATGTTGATTACAAACAACAGGTATGTGATGTTTTTGACTCAAAGGGTGCATATTTGAGAGATAGATTGTCTTTGCTGTGCATATGAAACTGAGAGGCTTTGGTTACCATATTTACTCTTCTTTTACATTCTACAACGTTTAAATGATGGGATTAAGCTTCTGCCTCATGTACATATCATCAATTGATGCTTTAGTCATTTATTGGGGAGTGATTGATGAATTGATTCAGGTCCAATGATCTGAAATTTGTCTTATGAACATTGAAAGAGGGAAATTACAAAACACACAAGTTATTAGTATATGAGCTGTGGTTTTTATTCTTTTGTTGTGAACAGTATGGAATTACAGCGTTACATACAAGTTTCAAT

Coding sequence (CDS)

ATGGGTCCAATCAGAGGGTTGAGAAAGAAGAAGAAATTAGAGAGAAAGATCGATTCCAATGGTACTGCTTCTGATTCTTCTGAAAAGGATGAGGCTATAGATTGGTGGGATGATTTCTCCAAACGAACCAATGGTCTTCATTCTGCATCAAAAGGGTTGGATAGATTCAAATCCATTTTCAAGGTCTCCCGAAAGACTTTTGATTACATATGTTTGCTTGTCAAGGACGATATGACAGCTAAATCTGGTCATTTTACATTTTTGAACGGTAGGCCGTTGTCTTTATGTGATCAAGTTGCTGTAGCTTTAAGAAGGCTGGGGTCCGGTGAATCATTAGTGACAATAGGTGATTCACTTGGATTGAACCACTCGACTGTGTCTCAAGTCACATGGCGATTTGTGGAGTCAATGGAAGAAAGGGGGCTTCGCCATCTTCATTGGCCTTCAAACGAAGTGGAAATGGCTCAAGTGAAATCAAAATTTGAGAAAATACAAGGACTTCCTAACTGTTGTGGTTCGATCGATGCCACTCACATCATGATGTGCCTGCCTGCTTCGGATCCTACAAGTTATGTGTGGCTTGATGATGAAAAAAACCACAGCATGGTTTTGCAAGTGATTGTAGATGCAGAAATGAGGTTCCGAGACATATTAACTGGATTGCCTGGAAAATTGTCGGATTGGTTAGTTTTCCAGAGTTCAAACTTCCACAAGCTTTGTGACAAGGGGGAGAGGCTGAATGGGAAGAGGTTAGAGCTTCCCGACAGATCGGAAATCCGAGAATATATAGTTGGAGATTCAGGCTATCCTTTACTTCCCTATCTTGTCACTCCCTATGATGGAAAAGAACTCTCAACATCAAAAGCCGAGTTCAACAAGCGACACAAAGAGACTCGGTTGGTGGTTCAACAAGCACTAGCAATGTTGAAAGAACGGTGGAGGATCATTCAAGGAGTGATGTGGAGACCCGATAAACATCGGTTGCCGAGAATCATTCTAGTCTGCTGCTTACTTCATAACATTATCATTGATATCGGAGACGAGACGGAGGATGGTGACATTCCTTTGTCTGTCGAACATGATGTTGATTACAAACAACAGGTATGTGATGTTTTTGACTCAAAGGGTGCATATTTGAGAGATAGATTGTCTTTGCTGTGCATATGA

Protein sequence

MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEHDVDYKQQVCDVFDSKGAYLRDRLSLLCI
Homology
BLAST of PI0008078.1 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 6.7e-130
Identity = 228/403 (56.58%), Postives = 294/403 (72.95%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSN----GTAS---------------DSSEKDEAIDWWDDFSK 60
           MGPI+ ++KKK+ E+K+D N     TA+               D     +++DWWD FS+
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAV 120
           R   ++  S     F+S+FK+SRKTFDYIC LVK D TAK  +F+  NG PLSL D+VAV
Sbjct: 61  R---IYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120

Query: 121 ALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKF 180
           ALRRLGSGESL  IG++ G+N STVSQ+TWRFVESMEER + HL WPS   ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180

Query: 181 EKIQGLPNCCGSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGL 240
           EKI GLPNCCG+ID THI+M LPA +P++ VWLD EKN SM LQ +VD +MRF D++ G 
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240

Query: 241 PGKLSDWLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDG 300
           PG L+D +V ++S F+KL +KG+RLNG++L L +R+E+REYIVGDSG+PLLP+L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300

Query: 301 KELSTSKAEFNKRHKETRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 360
           K  S  + EFNKRH E     Q AL+ LK+RWRII GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360

Query: 361 IIDIGDETEDGDIPLSVEHDVDYKQQVCDVFDSKGAYLRDRLS 385
           IID+ D+T D D PLS +HD++Y+Q+ C + D   + LRD LS
Sbjct: 361 IIDMEDQTLD-DQPLSQQHDMNYRQRSCKLADEASSVLRDELS 396

BLAST of PI0008078.1 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 6.1e-91
Identity = 181/397 (45.59%), Postives = 248/397 (62.47%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEK---------DEAI--DWWDDFSKRTNGLHSA 60
           M P++  +KKK  ++ +D     + + EK          EAI  DWWD F  R +     
Sbjct: 1   MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60

Query: 61  SKGLDRFKSIFKVSRKTFDYICLLVKDDMTAK--SGHFTFLNGRPLSLCDQVAVALRRLG 120
           S     FK  F+ S+ TF YIC LV++D+ ++  SG    + GR LS+  QVA+ALRRL 
Sbjct: 61  SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLIN-IEGRLLSVEKQVAIALRRLA 120

Query: 121 SGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGL 180
           SG+S V++G + G+  STVSQVTWRF+E++EER   HL WP ++  + ++KSKFE++ GL
Sbjct: 121 SGDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGL 180

Query: 181 PNCCGSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD 240
           PNCCG+ID THI+M LPA    S  W D EKN+SM LQ + D EMRF +++TG PG ++ 
Sbjct: 181 PNCCGAIDTTHIIMTLPAVQ-ASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTV 240

Query: 241 WLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTS 300
             + + S F KLC+  + L+G    L   ++IREY+VG   YPLLP+L+TP+D    S S
Sbjct: 241 SKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDS 300

Query: 301 KAEFNKRHKETRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD 360
              FN+RH++ R V   A   LK  WRI+  VMWRPD+ +LP IILVCCLLHNIIID GD
Sbjct: 301 MVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGD 360

Query: 361 ETEDGDIPLSVEHDVDYKQQVCDVFDSKGAYLRDRLS 385
             ++ D+PLS  HD  Y  + C   +  G+ LR  L+
Sbjct: 361 YLQE-DVPLSGHHDSGYADRYCKQTEPLGSELRGCLT 391

BLAST of PI0008078.1 vs. ExPASy Swiss-Prot
Match: Q6AZB8 (Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.4e-26
Identity = 85/310 (27.42%), Postives = 147/310 (47.42%), Query Frame = 0

Query: 51  KGLDRFK----------SIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVA 110
           K LDRF           + F   R+   Y+  L+KD +  ++        R +S   Q+ 
Sbjct: 20  KTLDRFDIETVSDDFLLNTFGFPREFIYYLVELLKDSLLRRT-----QRSRAISPDVQIL 79

Query: 111 VALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSK 170
            AL    SG     +GD++G++ +++S+      +++ E+    + +  +E    Q K +
Sbjct: 80  AALGFYTSGSFQSKMGDAIGISQASMSRCVSNVTKALIEKAPEFIGFTRDEATKQQFKDE 139

Query: 171 FEKIQGLPNCCGSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTG 230
           F +I G+PN  G +D  HI +  P +D +SYV  + +  HS+  Q++ DA        T 
Sbjct: 140 FYRIAGIPNVTGVVDCAHIAIKAPNADDSSYV--NKKGFHSINCQLVCDARGLLLSAETH 199

Query: 231 LPGKLSDWLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYD 290
            PG L+D  VF+ SN  KL ++ E            ++   +++GD+ YPL  +L+TP  
Sbjct: 200 WPGSLTDRAVFKQSNVAKLFEEQE------------NDDEGWLLGDNRYPLKKWLMTPVQ 259

Query: 291 GKELSTSKAEFNKRHKETRLVVQQALAMLKERWRIIQG----VMWRPDKHRLPRIILVCC 347
             E S +   +N  H  T  +V +    ++ R+R + G    + + P+K     II  CC
Sbjct: 260 SPE-SPADYRYNLAHTTTHEIVDRTFRAIQTRFRCLDGAKGYLQYSPEK--CSHIIQACC 307

BLAST of PI0008078.1 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 6.9e-26
Identity = 77/299 (25.75%), Postives = 145/299 (48.49%), Query Frame = 0

Query: 91  RPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLRHLHWPSN 150
           R +S   Q+  AL    SG     +GD++G++ +++S+      E++ ER  + +H+P++
Sbjct: 65  RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPAD 124

Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDA 210
           E  +  +K +F  + G+P   G++D  H+ +  P ++  SYV  + +  HS+   V+ D 
Sbjct: 125 EAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYV--NRKGLHSLNCLVVCDI 184

Query: 211 EMRFRDILTGLPGKLSDWLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYP 270
                 + T  PG L D  V Q S+     + G         +P  S    +++GDS + 
Sbjct: 185 RGALMTVETSWPGSLQDCAVLQQSSLSSQFETG---------MPKDS----WLLGDSSFF 244

Query: 271 LLPYLVTPYDGKELSTSKAEFNKRHKETRLVVQQALAMLKERWRIIQG----VMWRPDKH 330
           L  +L+TP    E + ++  +N+ H  T  V+++ L  L  R+R + G    + + P+K 
Sbjct: 245 LHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLDGSKGALQYSPEKS 304

Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEHDVDYKQQVCDVFDSKGAYLRDRLSL 386
               IIL CC+LHNI ++ G +     +   +E   + + +  +  D +   +R  L L
Sbjct: 305 --SHIILACCVLHNISLEHGMDVWSSPVTGPIEQPPEGEDEQMESLDLEADRIRQELIL 345

BLAST of PI0008078.1 vs. ExPASy Swiss-Prot
Match: Q8BR93 (Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.5e-25
Identity = 78/299 (26.09%), Postives = 143/299 (47.83%), Query Frame = 0

Query: 91  RPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLRHLHWPSN 150
           R +S   Q+  AL    SG     +GD++G++ +++S+      E++ ER  + +H+P +
Sbjct: 65  RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPVD 124

Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDA 210
           E  +  +K +F  + G+P   G  D  H+ +  P ++  SYV  + +  HS+   V+ D 
Sbjct: 125 EAAVQSLKDEFYGLAGMPGVIGVADCIHVAIKAPNAEDLSYV--NRKGLHSLNCLVVCDI 184

Query: 211 EMRFRDILTGLPGKLSDWLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYP 270
                 + T  PG L D  V Q S+     + G         +P  S    +++GDS + 
Sbjct: 185 RGALMTVETSWPGSLQDCAVLQRSSLTSQFETG---------MPKDS----WLLGDSSFF 244

Query: 271 LLPYLVTPYDGKELSTSKAEFNKRHKETRLVVQQALAMLKERWRIIQG----VMWRPDKH 330
           L  +L+TP    E + ++  +N+ H  T  V+++ L  L  R+R + G    + + P+K 
Sbjct: 245 LRSWLLTPLPIPE-TAAEYRYNRAHSATHSVIERTLQTLCCRFRCLDGSKGALQYSPEK- 304

Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEHDVDYKQQVCDVFDSKGAYLRDRLSL 386
               IIL CC+LHNI +D G +     +P  ++   + + +  +  D +   +R  L L
Sbjct: 305 -CSHIILACCVLHNISLDHGMDVWSSPVPGPIDQPPEGEDEHMESLDLEADRIRQELIL 345

BLAST of PI0008078.1 vs. ExPASy TrEMBL
Match: A0A0A0M0C2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 5.6e-220
Identity = 371/388 (95.62%), Postives = 381/388 (98.20%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+D NGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDCNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASDPTSYVWLDD+KNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKR ELPDRSEIREYI+GDSGYPLLPYLVTPYDGKELSTSK EFNKRHKETRL
Sbjct: 241 DKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEH 360
           VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETE+G++PLS+EH
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           DVDYKQQVCDVFDSKGAY+RDRLSLL I
Sbjct: 361 DVDYKQQVCDVFDSKGAYVRDRLSLLFI 388

BLAST of PI0008078.1 vs. ExPASy TrEMBL
Match: A0A5D3BNB9 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00830 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 4.7e-219
Identity = 374/388 (96.39%), Postives = 381/388 (98.20%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+DSNGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASDPTS+VWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKRLELPDRSEI+EYIVGDSGYPLL YLVTPYDGKELSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGD+PLS+EH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           DVDYKQQVCDVFDSKGAYLR+RLSLL I
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of PI0008078.1 vs. ExPASy TrEMBL
Match: A0A5A7SXL0 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43059G001000 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.0e-218
Identity = 373/388 (96.13%), Postives = 380/388 (97.94%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+DSNGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASDPTS+VWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKRLELPDRSEI+EYIVGDSGYPLL YLVTPYDGKELSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGD+PLS+EH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           DVDYKQQVCDVFDSKGAYLR+RLSLL I
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of PI0008078.1 vs. ExPASy TrEMBL
Match: A0A1S3C6F2 (LOW QUALITY PROTEIN: putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103497206 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 9.8e-217
Identity = 371/388 (95.62%), Postives = 378/388 (97.42%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+DSNGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASDPTS+VWLD  KNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDXRKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKRLELPDRSEI+EYIVGDSGYPLL YLVTPYDGKELSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGD+PLS+EH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           DVDYKQQVCDVFDSKGAYLR+RLSLL I
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of PI0008078.1 vs. ExPASy TrEMBL
Match: A0A6J1GBJ6 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111452471 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.9e-196
Identity = 336/389 (86.38%), Postives = 359/389 (92.29%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSN-GTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSI 60
           MGPIRG RKKKKLERK+D+N  TASDSSEKD+A+DWWDDFS+RT GLHS  +GLD FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSL 120
           FKVSRKTFDYICLLVKDDMTA+S +FTFLNGRPLSL DQVAVALRRLGSG+SLVTIG S 
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAESSNFTFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GLNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHI 180
           GLNHSTVSQVTWRFVESME RGLRHLHWPS E EMAQVK KFEKIQGLPNCCGSID THI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 MMCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKL 240
            MCLP  DPTS VWLD EKNHSMVLQVIVDAEMRFRDI+TGLPGK+SDWLVFQSSNFHKL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIVTGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CDKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETR 300
           C+KGERLNGKRLE  +RSEIREYI+GDSGYPLLPYLVTPYDGKEL  SKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 LVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVE 360
           LVVQ+ALA LKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIID+GDE EDG++P+S+E
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVPMSME 360

Query: 361 HDVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           HD DYKQQ+CDV+DSKGAYLRD+LSLL I
Sbjct: 361 HDADYKQQICDVYDSKGAYLRDKLSLLFI 389

BLAST of PI0008078.1 vs. NCBI nr
Match: XP_004149039.1 (protein ALP1-like [Cucumis sativus] >KGN65671.1 hypothetical protein Csa_019843 [Cucumis sativus])

HSP 1 Score: 773.1 bits (1995), Expect = 1.1e-219
Identity = 371/388 (95.62%), Postives = 381/388 (98.20%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+D NGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDCNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASDPTSYVWLDD+KNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKR ELPDRSEIREYI+GDSGYPLLPYLVTPYDGKELSTSK EFNKRHKETRL
Sbjct: 241 DKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEH 360
           VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETE+G++PLS+EH
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           DVDYKQQVCDVFDSKGAY+RDRLSLL I
Sbjct: 361 DVDYKQQVCDVFDSKGAYVRDRLSLLFI 388

BLAST of PI0008078.1 vs. NCBI nr
Match: TYK01291.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 770.0 bits (1987), Expect = 9.7e-219
Identity = 374/388 (96.39%), Postives = 381/388 (98.20%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+DSNGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASDPTS+VWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKRLELPDRSEI+EYIVGDSGYPLL YLVTPYDGKELSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGD+PLS+EH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           DVDYKQQVCDVFDSKGAYLR+RLSLL I
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of PI0008078.1 vs. NCBI nr
Match: KAA0033909.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 766.9 bits (1979), Expect = 8.2e-218
Identity = 373/388 (96.13%), Postives = 380/388 (97.94%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+DSNGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASDPTS+VWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKRLELPDRSEI+EYIVGDSGYPLL YLVTPYDGKELSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGD+PLS+EH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           DVDYKQQVCDVFDSKGAYLR+RLSLL I
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of PI0008078.1 vs. NCBI nr
Match: XP_038893506.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 765.0 bits (1974), Expect = 3.1e-217
Identity = 372/389 (95.63%), Postives = 380/389 (97.69%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+DSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASD TSYVWLD+EKNHSMVLQVIVDAEMRFRDILTGLPGK+SDWLVFQSSNFHKLC
Sbjct: 181 MCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMRFRDILTGLPGKMSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKRLEL DRSEIREYI+GDSGYPLLPYLVTPYDGKE STSKAEFNKRHKETRL
Sbjct: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKEHSTSKAEFNKRHKETRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDET-EDGDIPLSVE 360
           VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD+T EDG +PLS+E
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDDTEEDGGVPLSIE 360

Query: 361 HDVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           HDVDYKQQVCDVFD KGAYLRDRLSLL I
Sbjct: 361 HDVDYKQQVCDVFDPKGAYLRDRLSLLFI 389

BLAST of PI0008078.1 vs. NCBI nr
Match: XP_008457540.1 (PREDICTED: LOW QUALITY PROTEIN: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 762.3 bits (1967), Expect = 2.0e-216
Identity = 371/388 (95.62%), Postives = 378/388 (97.42%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERK+DSNGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIM 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSID THI 
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
           MCLPASDPTS+VWLD  KNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDXRKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRL 300
           DKGERLNGKRLELPDRSEI+EYIVGDSGYPLL YLVTPYDGKELSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGD+PLS+EH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDRLSLLCI 389
           DVDYKQQVCDVFDSKGAYLR+RLSLL I
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of PI0008078.1 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 465.3 bits (1196), Expect = 4.8e-131
Identity = 228/403 (56.58%), Postives = 294/403 (72.95%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSN----GTAS---------------DSSEKDEAIDWWDDFSK 60
           MGPI+ ++KKK+ E+K+D N     TA+               D     +++DWWD FS+
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAV 120
           R   ++  S     F+S+FK+SRKTFDYIC LVK D TAK  +F+  NG PLSL D+VAV
Sbjct: 61  R---IYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120

Query: 121 ALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKF 180
           ALRRLGSGESL  IG++ G+N STVSQ+TWRFVESMEER + HL WPS   ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180

Query: 181 EKIQGLPNCCGSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGL 240
           EKI GLPNCCG+ID THI+M LPA +P++ VWLD EKN SM LQ +VD +MRF D++ G 
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240

Query: 241 PGKLSDWLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDG 300
           PG L+D +V ++S F+KL +KG+RLNG++L L +R+E+REYIVGDSG+PLLP+L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300

Query: 301 KELSTSKAEFNKRHKETRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 360
           K  S  + EFNKRH E     Q AL+ LK+RWRII GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360

Query: 361 IIDIGDETEDGDIPLSVEHDVDYKQQVCDVFDSKGAYLRDRLS 385
           IID+ D+T D D PLS +HD++Y+Q+ C + D   + LRD LS
Sbjct: 361 IIDMEDQTLD-DQPLSQQHDMNYRQRSCKLADEASSVLRDELS 396

BLAST of PI0008078.1 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 335.9 bits (860), Expect = 4.4e-92
Identity = 181/397 (45.59%), Postives = 248/397 (62.47%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKIDSNGTASDSSEK---------DEAI--DWWDDFSKRTNGLHSA 60
           M P++  +KKK  ++ +D     + + EK          EAI  DWWD F  R +     
Sbjct: 1   MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60

Query: 61  SKGLDRFKSIFKVSRKTFDYICLLVKDDMTAK--SGHFTFLNGRPLSLCDQVAVALRRLG 120
           S     FK  F+ S+ TF YIC LV++D+ ++  SG    + GR LS+  QVA+ALRRL 
Sbjct: 61  SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLIN-IEGRLLSVEKQVAIALRRLA 120

Query: 121 SGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGL 180
           SG+S V++G + G+  STVSQVTWRF+E++EER   HL WP ++  + ++KSKFE++ GL
Sbjct: 121 SGDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGL 180

Query: 181 PNCCGSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD 240
           PNCCG+ID THI+M LPA    S  W D EKN+SM LQ + D EMRF +++TG PG ++ 
Sbjct: 181 PNCCGAIDTTHIIMTLPAVQ-ASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTV 240

Query: 241 WLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTS 300
             + + S F KLC+  + L+G    L   ++IREY+VG   YPLLP+L+TP+D    S S
Sbjct: 241 SKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDS 300

Query: 301 KAEFNKRHKETRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD 360
              FN+RH++ R V   A   LK  WRI+  VMWRPD+ +LP IILVCCLLHNIIID GD
Sbjct: 301 MVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGD 360

Query: 361 ETEDGDIPLSVEHDVDYKQQVCDVFDSKGAYLRDRLS 385
             ++ D+PLS  HD  Y  + C   +  G+ LR  L+
Sbjct: 361 YLQE-DVPLSGHHDSGYADRYCKQTEPLGSELRGCLT 391

BLAST of PI0008078.1 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 134.0 bits (336), Expect = 2.5e-31
Identity = 86/339 (25.37%), Postives = 164/339 (48.38%), Query Frame = 0

Query: 29  KDEAIDWWDDFSKRTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFL 88
           KD +  WW++ S+            + FK  F++S+ TF+ IC    D++ +        
Sbjct: 155 KDRSRAWWEECSR-------LDYPEEDFKKAFRMSKSTFELIC----DELNSAVAKEDTA 214

Query: 89  NGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGL-RHLHW 148
               + +  +VAV + RL +GE L  +    GL  ST  ++     +++++  + ++L W
Sbjct: 215 LRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQW 274

Query: 149 PSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIMMCLPASDPTSY-----VWLDDEKNHSM 208
           P +E  +  ++ +FE + G+PN  GS+  THI +  P     SY        + + ++S+
Sbjct: 275 PDDE-SLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTERNQKTSYSI 334

Query: 209 VLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREY 268
            +Q +V+ +  F D+  G PG + D  V + S  ++  + G  L G             +
Sbjct: 335 TIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGM------------W 394

Query: 269 IVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRLVVQQALAMLKERWRIIQGVMWR 328
           + G  G+PLL +++ PY  + L+ ++  FN++  E + V ++A   LK RW  +Q     
Sbjct: 395 VAGGPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQ-KRTE 454

Query: 329 PDKHRLPRIILVCCLLHNIIIDIGDETEDGDIPLSVEHD 362
                LP ++  CC+LHN I ++ +E  + ++ + V  D
Sbjct: 455 VKLQDLPTVLGACCVLHN-ICEMREEKMEPELMVEVIDD 467

BLAST of PI0008078.1 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 125.6 bits (314), Expect = 9.0e-29
Identity = 92/319 (28.84%), Postives = 152/319 (47.65%), Query Frame = 0

Query: 29  KDEAIDWWDDFSKRTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFL 88
           K+   DWWD  S+            D F+  F++S+ TF+ IC  +   +T K+   T L
Sbjct: 193 KERTTDWWDRVSR-------PDFPEDEFRREFRMSKSTFNLICEELDTTVTKKN---TML 252

Query: 89  NGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGL-RHLHW 148
               +    +V V + RL +G  L  + +  GL  ST  ++      ++ +  + ++L W
Sbjct: 253 RD-AIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLW 312

Query: 149 PSNEVEMAQVKSKFEKIQGLPNCCGSIDATHIMMCLPASDPTSY-----VWLDDEKNHSM 208
           PS+  E+   K+KFE +  +PN  GSI  THI +  P     +Y        + + ++S+
Sbjct: 313 PSDS-EINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSI 372

Query: 209 VLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLCDKGERLNGKRLELPDRSEIREY 268
            +Q +V+A+  F D+  G PG L+D  + + S+         R    R  L D      +
Sbjct: 373 TVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSL-------SRQRAARGMLRD-----SW 432

Query: 269 IVGDSGYPLLPYLVTPYDGKELSTSKAEFNKRHKETRLVVQQALAMLKERWRIIQGVMWR 328
           IVG+SG+PL  YL+ PY  + L+ ++  FN+   E + +   A   LK RW  +Q     
Sbjct: 433 IVGNSGFPLTDYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQ-KRTE 486

Query: 329 PDKHRLPRIILVCCLLHNI 342
                LP ++  CC+LHNI
Sbjct: 493 VKLQDLPYVLGACCVLHNI 486

BLAST of PI0008078.1 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 104.0 bits (258), Expect = 2.8e-22
Identity = 79/291 (27.15%), Postives = 136/291 (46.74%), Query Frame = 0

Query: 55  RFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVA--LRRLGSGESL 114
           R++S++ +S   F  +   +K  +TA +          LSL    AVA  L RL  G S 
Sbjct: 116 RWRSLYGLSYPVFITVVDKLKPFITASN----------LSLPADYAVAMVLSRLAHGCSA 175

Query: 115 VTIGDSLGLNHSTVSQVTWRFVESMEERGL-RHLHWPSNEVEMAQVKSKFEKIQGLPNCC 174
            T+     L+   +S++T      +  +     +  P  +  + +    FE++  LPN C
Sbjct: 176 KTLASRYSLDPYLISKITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNIC 235

Query: 175 GSIDATHIMMCLPASDPTSYVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVF 234
           G+ID+T + +          ++       +++LQV+ D +  F D+    PG   D   F
Sbjct: 236 GAIDSTPVKLRRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPGGEDDSSHF 295

Query: 235 QSSNFHKLCDKGERLNGKRLELPDRSEIREYIVGDSGYPLLPYLVTPYDGKELSTSKAE- 294
           + S  +K    G+ +  K + +     +R YIVGD  YPLL +L+TP+      T     
Sbjct: 296 RDSLLYKRLTSGDIVWEKVINIRGH-HVRPYIVGDWCYPLLSFLMTPFSPNGSGTPPENL 355

Query: 295 FNKRHKETRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 342
           F+    + R VV +A+ +LK RW+I+Q +      +  P+ I+ CC+LHN+
Sbjct: 356 FDGMLMKGRSVVVEAIGLLKARWKILQSL--NVGVNHAPQTIVACCVLHNL 393

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2U36.7e-13056.58Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
Q94K496.1e-9145.59Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=... [more]
Q6AZB82.4e-2627.42Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1[more]
B0BN956.9e-2625.75Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1[more]
Q8BR931.5e-2526.09Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M0C25.6e-22095.62DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE... [more]
A0A5D3BNB94.7e-21996.39Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7SXL04.0e-21896.13Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3C6F29.8e-21795.62LOW QUALITY PROTEIN: putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A6J1GBJ61.9e-19686.38protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111452471 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004149039.11.1e-21995.62protein ALP1-like [Cucumis sativus] >KGN65671.1 hypothetical protein Csa_019843 ... [more]
TYK01291.19.7e-21996.39putative nuclease HARBI1 [Cucumis melo var. makuwa][more]
KAA0033909.18.2e-21896.13putative nuclease HARBI1 [Cucumis melo var. makuwa][more]
XP_038893506.13.1e-21795.63protein ALP1-like [Benincasa hispida][more]
XP_008457540.12.0e-21695.62PREDICTED: LOW QUALITY PROTEIN: putative nuclease HARBI1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G55350.14.8e-13156.58PIF / Ping-Pong family of plant transposases [more]
AT3G63270.14.4e-9245.59CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT5G12010.12.5e-3125.37unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, ... [more]
AT4G29780.19.0e-2928.84unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G19120.12.8e-2227.15PIF / Ping-Pong family of plant transposases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 174..340
e-value: 1.8E-27
score: 96.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 1..386
NoneNo IPR availablePANTHERPTHR22930:SF194NUCLEASE HARBI1 ISOFORM X1-RELATEDcoord: 1..386

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
PI0008078PI0008078gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0008078.1-exonPI0008078.1-exon-chr12:23059177..23060492exon
PI0008078.1-exonPI0008078.1-exon-chr12:23061886..23062306exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0008078.1-three_prime_utrPI0008078.1-three_prime_utr-chr12:23059177..23059458three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0008078.1-cdsPI0008078.1-cds-chr12:23059459..23060492CDS
PI0008078.1-cdsPI0008078.1-cds-chr12:23061886..23062018CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0008078.1-five_prime_utrPI0008078.1-five_prime_utr-chr12:23062019..23062306five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
PI0008078.1PI0008078.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding