Moc08g00270.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g00270.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	BOI-related E3 ubiquitin-protein ligase 1
Location	chr8: 165572 .. 167053 (-)
Sequence length	1020
RNA-Seq Expression	Moc08g00270.1
Synteny	Moc08g00270.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGGTGGTGACAATGGCAATGCCTTGCTTCCCATTTTCCTGGATGAAAATCGAACTCAGTATCAAAGTAATGCTTCTGGTCAGCTGCAGCTATTTGGAAATTGTGAGTTGTTTTTTCTGTTTCTTAGAAATCTGATTTCAACTTAATTCATTCCATATATTTTGTTATTATGTTTAACCATTAAAAATTATTTGAGTTAAAGTTTGATCAGTAGAAGATTTAACGAGATCAATCTGAACTTGTTAAATGTCCTTTAGTTGTCACGTCTCTAATAAAATTCACAATTTATATTGTAGTTCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAGTGAGCAAATGGCTCATATGCTCCGCCCTAACAAAAGAAGTAGGGAAGTAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAACGCCTGTCCAGATGATGCTGATCGTTCAGCAAACATTAGAAACCCAAATGCCGTGTCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACTACTGCAAGTGGAAGTATAACAGCGGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAGTTTGATCAGTATATTAAAATTCAGGTACTTCTAAACTCCTCGGAGTTTCTAGGACTTATTCCTCGTCTGGATCTTTACAAGAGCACATGGGGATAAAACTAGTATAGTTTATGATGAGGTTTCCTGGAAGATGTTTCTGGTCAAAGTCTAATTGGTTTATGAACTATGTATTACTATTTATAGCATTAACTTCACACGATGCCTGCCAAATGGTGCTCATGTTGAAGTATTCTTCAATGCGGTTGATTCAATTGATCTGTGGTTTTAAAATTTTCATATGCCTTACAAACAGGAAGAACATTTGGCAAAGGGTATAAGGGACATGAAGCAGAGACATACGGCCTCGTTTCTTAATGCAATAGAGAAAGGGATCGGGAGGAAACTACATGAGAAGGATGTTGAAATTGAGAATATGAACCGTAAAAACAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCGACGGAAGCCCAGAATTGGCATAGCAGAGCTAAATACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTCCAGCATGCTATCTCGCAGGGTGGGGACCAAGCAAAGGAAGGATTCGGAGACAGTGAGGTGGATGATGCTGCCTCACACGTTGATCCAAACAATTACCTTAGTGTTCCAGGAGGGAATGTGAAATCTTGTACCAGGAATTTACCAGGCTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTCATGCCTTGTAGACACCTTTGTTTGTGTAAAGATTGTGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGCATTCAAGTGTATTTGTCCTAA

mRNA sequence

ATGTTGGGTGGTGACAATGGCAATGCCTTGCTTCCCATTTTCCTGGATGAAAATCGAACTCAGTATCAAAGTAATGCTTCTGGTCAGCTGCAGCTATTTGGAAATTTTCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAGTGAGCAAATGGCTCATATGCTCCGCCCTAACAAAAGAAGTAGGGAAGTAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAACGCCTGTCCAGATGATGCTGATCGTTCAGCAAACATTAGAAACCCAAATGCCGTGTCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACTACTGCAAGTGGAAGTATAACAGCGGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAGTTTGATCAGTATATTAAAATTCAGGAAGAACATTTGGCAAAGGGTATAAGGGACATGAAGCAGAGACATACGGCCTCGTTTCTTAATGCAATAGAGAAAGGGATCGGGAGGAAACTACATGAGAAGGATGTTGAAATTGAGAATATGAACCGTAAAAACAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCGACGGAAGCCCAGAATTGGCATAGCAGAGCTAAATACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTCCAGCATGCTATCTCGCAGGGTGGGGACCAAGCAAAGGAAGGATTCGGAGACAGTGAGGTGGATGATGCTGCCTCACACGTTGATCCAAACAATTACCTTAGTGTTCCAGGAGGGAATGTGAAATCTTGTACCAGGAATTTACCAGGCTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTCATGCCTTGTAGACACCTTTGTTTGTGTAAAGATTGTGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGCATTCAAGTGTATTTGTCCTAA

Coding sequence (CDS)

ATGTTGGGTGGTGACAATGGCAATGCCTTGCTTCCCATTTTCCTGGATGAAAATCGAACTCAGTATCAAAGTAATGCTTCTGGTCAGCTGCAGCTATTTGGAAATTTTCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAGTGAGCAAATGGCTCATATGCTCCGCCCTAACAAAAGAAGTAGGGAAGTAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAACGCCTGTCCAGATGATGCTGATCGTTCAGCAAACATTAGAAACCCAAATGCCGTGTCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACTACTGCAAGTGGAAGTATAACAGCGGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAGTTTGATCAGTATATTAAAATTCAGGAAGAACATTTGGCAAAGGGTATAAGGGACATGAAGCAGAGACATACGGCCTCGTTTCTTAATGCAATAGAGAAAGGGATCGGGAGGAAACTACATGAGAAGGATGTTGAAATTGAGAATATGAACCGTAAAAACAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCGACGGAAGCCCAGAATTGGCATAGCAGAGCTAAATACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTCCAGCATGCTATCTCGCAGGGTGGGGACCAAGCAAAGGAAGGATTCGGAGACAGTGAGGTGGATGATGCTGCCTCACACGTTGATCCAAACAATTACCTTAGTGTTCCAGGAGGGAATGTGAAATCTTGTACCAGGAATTTACCAGGCTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTCATGCCTTGTAGACACCTTTGTTTGTGTAAAGATTGTGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGCATTCAAGTGTATTTGTCCTAA

Protein sequence

MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPNKRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAISQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS

Homology

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BLAST of Moc08g00270.1 vs. NCBI nr
Match: XP_022132217.1 (BOI-related E3 ubiquitin-protein ligase 1 [Momordica charantia])

HSP 1 Score: 680.2 bits (1754), Expect = 8.8e-192
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN
Sbjct: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE
Sbjct: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV
Sbjct: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. NCBI nr
Match: XP_022997618.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.1 BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_023545645.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545646.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545647.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.2 bits (1611), Expect = 3.4e-175
Identity = 309/339 (91.15%), Postives = 325/339 (95.87%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+ AAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. NCBI nr
Match: XP_022961638.1 (BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961639.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961640.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata])

HSP 1 Score: 624.0 bits (1608), Expect = 7.5e-175
Identity = 308/339 (90.86%), Postives = 324/339 (95.58%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHVASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. NCBI nr
Match: KAG6598221.1 (putative BOI-related E3 ubiquitin-protein ligase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 623.6 bits (1607), Expect = 9.8e-175
Identity = 308/339 (90.86%), Postives = 324/339 (95.58%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. NCBI nr
Match: XP_038884634.1 (probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida])

HSP 1 Score: 623.6 bits (1607), Expect = 9.8e-175
Identity = 309/339 (91.15%), Postives = 325/339 (95.87%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+FLDENRTQYQ+NASGQLQLFGN PVSCGIEPVNYFG+EQ+ HMLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQIVHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFSRQQKLQISLNY AC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLNY-ACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+TAAPSII SLGDNIRTEIDRQKEEFDQYIK+QEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMTAAPSIILSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIK VATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAAS++DPNNYLSVPGG +KS T NLPGFKEHMICRACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKSSTSNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVC LIKTAGIQV+LS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVFLS 338

[Close]

BLAST of Moc08g00270.1 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.3e-22
Identity = 74/244 (30.33%), Postives = 123/244 (50.41%), Query Frame = 0


Query: 115 SSVTTASGSITAAPSIIFSLGD--NIRTEIDRQKEEFDQYIKI---QEEHLAKGIRDMKQ 174
           SS    + + T   ++   LG   +I + +++Q+ E D+++ +   Q E +   I + ++
Sbjct: 120 SSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRK 179

Query: 175 RHTASFLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVV 234
           R   + + AIE+G+ ++L  K+ E E + + N  L ER+K ++ E Q W   A+ NE+  
Sbjct: 180 RQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATA 239

Query: 235 NVLKNNLQHAISQGGDQAKEGFG----DSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPG 294
           N L+ NL+H ++Q  D ++ G G     +E DDA S        S  GG  +   R   G
Sbjct: 240 NHLRTNLEHVLAQVKDVSR-GAGLEKNMNEEDDAESCCG-----SSCGGGGEETVRRRVG 299

Query: 295 F----------KEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQ 340
                      +   +CR C  +E  +LL+PCRHLCLC  C   V+ CP+C   K A + 
Sbjct: 300 LEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVH 357

[Close]

BLAST of Moc08g00270.1 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.5e-19
Identity = 62/217 (28.57%), Postives = 104/217 (47.93%), Query Frame = 0


Query: 134 LGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIEKGIGRKLHEKDVE 193
           LG ++ + + +   + D+ I    E +   I + ++      + A+E+G+ + L  KD E
Sbjct: 126 LGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDE 185

Query: 194 IENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAIS-----------Q 253
           I ++ + N  L E++K +  E Q W   A+ NE+ VN L++NLQ  ++            
Sbjct: 186 INHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEPPT 245

Query: 254 GGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEVSI 313
             D A+   G +  D+  S  +            K C   +       +CR+C   E S+
Sbjct: 246 VADDAQSCCGSN--DEGDSEEERWKLAGEAQDTKKMCRVGM------SMCRSCGKGEASV 305

Query: 314 LLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           LL+PCRH+CLC  C   +N CP+C+  KTA + V LS
Sbjct: 306 LLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

[Close]

BLAST of Moc08g00270.1 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 9.3e-19
Identity = 66/203 (32.51%), Postives = 100/203 (49.26%), Query Frame = 0


Query: 140 TEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIEKGIGRKLHEKDVEIENMNR 199
           ++I +Q  E D+++  Q E L   +   ++  T    +A++  I +KL  KD EI  M +
Sbjct: 104 SQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGK 163

Query: 200 KNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAISQGGD-QAKEGFGDSEVDD 259
            N  L ER+K +  E Q W   A+ NE+  N L++NL+  ++Q  D  A       E DD
Sbjct: 164 LNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADD 223

Query: 260 AASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEVSILLMPCRHLCLCKDC-- 319
           A S        S  GG+V +             C+ C     S+L++PCRHLCLC  C  
Sbjct: 224 AESSCG-----SCDGGDVTAVVNG--------GCKRCGELTASVLVLPCRHLCLCTVCGS 283

Query: 320 DGLVNVCPVCQLIKTAGIQVYLS 340
             L+  CPVC ++ TA + V +S
Sbjct: 284 SALLRTCPVCDMVMTASVHVNMS 293

[Close]

BLAST of Moc08g00270.1 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.7e-18
Identity = 82/274 (29.93%), Postives = 130/274 (47.45%), Query Frame = 0


Query: 73  QKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTASGSI-TAAPSII 132
           + L    N+N    ++  S N   P  +ST  +  +   + N+ V   S +  +++PS+I
Sbjct: 51  EPLAAKANFNKA--ESGLSYNFTVP-PLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLI 110

Query: 133 -FSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIEKGIGRKLHEK 192
              L   I+   ++Q+ E D+++  Q E L   I   +Q  T    +A++  I +KL EK
Sbjct: 111 NVELVSQIQ---NQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEK 170

Query: 193 DVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAISQGGDQAKEG 252
           D EI  +   N  L ER+K +  E Q W   A+ NE+  N L+ NL   ++Q        
Sbjct: 171 DDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQ-------- 230

Query: 253 FGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMI---CRACKAKEVSILLMPC 312
               E    AS V  ++  S  G    SC  +  G     +   C+ C  +E S+L++PC
Sbjct: 231 ---LETFPTASAVVEDDAESSCG----SCCGDGGGEAVTAVGGGCKRCGEREASVLVLPC 290

Query: 313 RHLCLCKDCDG--LVNVCPVCQLIKTAGIQVYLS 340
           RHLCLC  C G  L+  CPVC ++  A + V +S
Sbjct: 291 RHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of Moc08g00270.1 vs. ExPASy Swiss-Prot
Match: O62640 (Putative inhibitor of apoptosis OS=Sus scrofa OX=9823 GN=PIAP PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.1e-06
Identity = 26/71 (36.62%), Postives = 38/71 (53.52%), Query Frame = 0


Query: 271 VPGGNVK--SCTRNLPGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQL 330
           +P  NV   S    L   +E   C+ C  KEVSI+ +PC HL +CKDC   +  CP+C+ 
Sbjct: 288 IPTENVSDLSMEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRG 347

Query: 331 IKTAGIQVYLS 340
                ++ +LS
Sbjct: 348 TIKGTVRTFLS 358

[Close]

BLAST of Moc08g00270.1 vs. ExPASy TrEMBL
Match: A0A6J1BT85 (BOI-related E3 ubiquitin-protein ligase 1 OS=Momordica charantia OX=3673 GN=LOC111005133 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 4.3e-192
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN
Sbjct: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE
Sbjct: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV
Sbjct: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. ExPASy TrEMBL
Match: A0A6J1KC06 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492498 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.6e-175
Identity = 309/339 (91.15%), Postives = 325/339 (95.87%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+ AAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. ExPASy TrEMBL
Match: A0A6J1HCD7 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC111462352 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.6e-175
Identity = 308/339 (90.86%), Postives = 324/339 (95.58%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHVASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. ExPASy TrEMBL
Match: A0A6J1GH18 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454110 PE=4 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 304/339 (89.68%), Postives = 321/339 (94.69%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGGDNGNA+LP+FLDENRTQYQ+NASGQLQLFGN PVSCGIEPVNYFG+EQMAHMLRPN
Sbjct: 1   MLGGDNGNAMLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFSRQQKLQISL+YNAC DDADR A+I NPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLDYNACQDDADRPASIINPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+TAAPSIIFSLGDN+R EIDRQKEE DQYIKIQEEHLAKGIR+MKQRH ASF+NAIE
Sbjct: 121 SGSMTAAPSIIFSLGDNLRIEIDRQKEELDQYIKIQEEHLAKGIREMKQRHMASFVNAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIENMN KNRELVERIKQV TEAQNW  RAKYNESVVNVLK+NLQH I
Sbjct: 181 KGIGKKLHEKDVEIENMNCKNRELVERIKQVGTEAQNWQCRAKYNESVVNVLKSNLQHVI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASHVD NNYLSVPGG ++S TRNL GFKE MICRACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHVDSNNYLSVPGGTMRSTTRNLAGFKERMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDC+GLV+VCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCNGLVSVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. ExPASy TrEMBL
Match: A0A6J1KPW7 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496531 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 301/339 (88.79%), Postives = 319/339 (94.10%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGGDNGNA+LP+FLDENRTQYQ+NASGQLQLFGN PVSCGIEPVNYFG+EQMAHMLRPN
Sbjct: 1   MLGGDNGNAMLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFSRQQKLQISL+YNAC DDADR A+I NPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLDYNACQDDADRPASIINPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+TAAPSIIFSLGDN+R EIDRQKEE DQYIKIQEEHLAKGIR+MKQRH ASF+NAIE
Sbjct: 121 SGSMTAAPSIIFSLGDNLRIEIDRQKEELDQYIKIQEEHLAKGIREMKQRHMASFVNAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIENMN KNRELVERIKQV TEAQNW  RAKYNESVVNVLK+NLQH I
Sbjct: 181 KGIGKKLHEKDVEIENMNCKNRELVERIKQVGTEAQNWQCRAKYNESVVNVLKSNLQHVI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+D NNYLSVPGG ++S TRNL GFKE MICRACK KEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDSNNYLSVPGGTMRSSTRNLAGFKERMICRACKEKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDC+GL +VCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCNGLASVCPVCQLIKTAGIQVYLS 339

[Close]

BLAST of Moc08g00270.1 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.2e-103
Identity = 198/352 (56.25%), Postives = 247/352 (70.17%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSN-ASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRP 60
           ML G+NGN   P+F++EN+ QYQ+N  S QL L GN    C ++PVNYF ++ +  M+RP
Sbjct: 1   MLSGNNGNTAPPVFMNENQLQYQTNLRSNQLHLLGNMGGGCTVDPVNYFANDNLVPMIRP 60

Query: 61  NKRSREVEDFS-----RQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHN 120
           NKR RE E  S     +QQKLQ+SLNYN   ++      +   N VSTGLRLSYDDDEHN
Sbjct: 61  NKRGREAESISNNIQRQQQKLQMSLNYNY--NNTSVREEVPKENLVSTGLRLSYDDDEHN 120

Query: 121 SSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTAS 180
           SSVT+ASGSI AA  I  SL D++R ++ RQK+EFDQ+IKIQ   +AKG+RDMKQRH AS
Sbjct: 121 SSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRHIAS 180

Query: 181 FLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKN 240
           FL  +EKG+ +KL EKD EI +MN+KN+ELVERIKQVA EAQNWH RAKYNESVVNVLK 
Sbjct: 181 FLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNVLKA 240

Query: 241 NLQHAISQ------GGDQAKEGFGDSEVDDAA-SHVDPNNYLSVPGGNVKSCTRNLPGFK 300
           NLQ A+S         DQ KEGFGDSE+DDAA S++DPNN              N  G  
Sbjct: 241 NLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNNN-----------NNNNMGIH 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           + M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC+ +K++ +QV+ S
Sbjct: 301 QRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 339

[Close]

BLAST of Moc08g00270.1 vs. TAIR 10
Match: AT1G60610.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.2e-92
Identity = 185/354 (52.26%), Postives = 238/354 (67.23%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNAS-GQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRP 60
           MLGG+N N +  + +++++ +YQSN S  QL L G     C I+PVNYF ++ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREVED---FSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH  SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNN 240
           + A+EK + +KL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGGD------------QAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG         +
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---------M 300

Query: 301 PGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
           P     M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 P--STGMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

[Close]

BLAST of Moc08g00270.1 vs. TAIR 10
Match: AT1G60610.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.2e-92
Identity = 185/354 (52.26%), Postives = 238/354 (67.23%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNAS-GQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRP 60
           MLGG+N N +  + +++++ +YQSN S  QL L G     C I+PVNYF ++ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREVED---FSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH  SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNN 240
           + A+EK + +KL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGGD------------QAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG         +
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---------M 300

Query: 301 PGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
           P     M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 P--STGMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

[Close]

BLAST of Moc08g00270.1 vs. TAIR 10
Match: AT1G60610.3 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.2e-92
Identity = 185/354 (52.26%), Postives = 238/354 (67.23%), Query Frame = 0


Query: 1   MLGGDNGNALLPIFLDENRTQYQSNAS-GQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRP 60
           MLGG+N N +  + +++++ +YQSN S  QL L G     C I+PVNYF ++ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREVED---FSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH  SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNN 240
           + A+EK + +KL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGGD------------QAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG         +
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---------M 300

Query: 301 PGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
           P     M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 P--STGMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

[Close]

BLAST of Moc08g00270.1 vs. TAIR 10
Match: AT1G10650.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.8e-86
Identity = 172/296 (58.11%), Postives = 210/296 (70.95%), Query Frame = 0


Query: 56  MLRPNKRSREVEDFS-----RQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDD 115
           M+RPNKR RE E  S     +QQKLQ+SLNYN   ++      +   N VSTGLRLSYDD
Sbjct: 1   MIRPNKRGREAESISNNIQRQQQKLQMSLNYNY--NNTSVREEVPKENLVSTGLRLSYDD 60

Query: 116 DEHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQR 175
           DEHNSSVT+ASGSI AA  I  SL D++R ++ RQK+EFDQ+IKIQ   +AKG+RDMKQR
Sbjct: 61  DEHNSSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQR 120

Query: 176 HTASFLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVN 235
           H ASFL  +EKG+ +KL EKD EI +MN+KN+ELVERIKQVA EAQNWH RAKYNESVVN
Sbjct: 121 HIASFLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVN 180

Query: 236 VLKNNLQHAISQ------GGDQAKEGFGDSEVDDAA-SHVDPNNYLSVPGGNVKSCTRNL 295
           VLK NLQ A+S         DQ KEGFGDSE+DDAA S++DPNN              N 
Sbjct: 181 VLKANLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNNN-----------NNNN 240

Query: 296 PGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
            G  + M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC+ +K++ +QV+ S
Sbjct: 241 MGIHQRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 283

The following BLAST results are available for this feature:

BLAST of Moc08g00270.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132217.1	8.8e-192	100.00	BOI-related E3 ubiquitin-protein ligase 1 [Momordica charantia]	[more]
XP_022997618.1	3.4e-175	91.15	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619....	[more]
XP_022961638.1	7.5e-175	90.86	BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961639.1 B...	[more]
KAG6598221.1	9.8e-175	90.86	putative BOI-related E3 ubiquitin-protein ligase 2, partial [Cucurbita argyrospe...	[more]
XP_038884634.1	9.8e-175	91.15	probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida]	[more]

BLAST of Moc08g00270.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IDI6	5.3e-22	30.33	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9LDD1	2.5e-19	28.57	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
Q9FHE4	9.3e-19	32.51	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
O81851	2.7e-18	29.93	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
O62640	4.1e-06	36.62	Putative inhibitor of apoptosis OS=Sus scrofa OX=9823 GN=PIAP PE=2 SV=1	[more]

BLAST of Moc08g00270.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BT85	4.3e-192	100.00	BOI-related E3 ubiquitin-protein ligase 1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1KC06	1.6e-175	91.15	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1HCD7	3.6e-175	90.86	BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1GH18	1.1e-171	89.68	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita mosch...	[more]
A0A6J1KPW7	1.6e-170	88.79	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxim...	[more]

BLAST of Moc08g00270.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10650.1	2.2e-103	56.25	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G60610.1	2.2e-92	52.26	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G60610.2	2.2e-92	52.26	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G60610.3	2.2e-92	52.26	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G10650.2	1.8e-86	58.11	SBP (S-ribonuclease binding protein) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 187..214
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 290..327 e-value: 4.8E-7 score: 29.5
None	No IPR available	PANTHER	PTHR42647:SF9	S-RIBONUCLEASE BINDING PROTEIN SBP1-RELATED	coord: 4..338
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 277..339 e-value: 4.0E-8 score: 34.6
IPR017066	E3 ubiquitin-protein ligase BOI-like	PIRSF	PIRSF036836	SBP1_RNase_bind	coord: 1..339 e-value: 4.8E-98 score: 326.9
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 4..338
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 292..327 score: 8.963311

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc08g00270	Moc08g00270	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc08g00270.1.exon3	Moc08g00270.1.exon3	exon
Moc08g00270.1.exon2	Moc08g00270.1.exon2	exon
Moc08g00270.1.exon1	Moc08g00270.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc08g00270.1	cds.Moc08g00270.1_3	CDS
cds.Moc08g00270.1	cds.Moc08g00270.1_2	CDS
cds.Moc08g00270.1	cds.Moc08g00270.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc08g00270.1	Moc08g00270.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity