Moc08g00270 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g00270
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionBOI-related E3 ubiquitin-protein ligase 1
Locationchr8: 165572 .. 167053 (-)
RNA-Seq ExpressionMoc08g00270
SyntenyMoc08g00270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGGTGGTGACAATGGCAATGCCTTGCTTCCCATTTTCCTGGATGAAAATCGAACTCAGTATCAAAGTAATGCTTCTGGTCAGCTGCAGCTATTTGGAAATTGTGAGTTGTTTTTTCTGTTTCTTAGAAATCTGATTTCAACTTAATTCATTCCATATATTTTGTTATTATGTTTAACCATTAAAAATTATTTGAGTTAAAGTTTGATCAGTAGAAGATTTAACGAGATCAATCTGAACTTGTTAAATGTCCTTTAGTTGTCACGTCTCTAATAAAATTCACAATTTATATTGTAGTTCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAGTGAGCAAATGGCTCATATGCTCCGCCCTAACAAAAGAAGTAGGGAAGTAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAACGCCTGTCCAGATGATGCTGATCGTTCAGCAAACATTAGAAACCCAAATGCCGTGTCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACTACTGCAAGTGGAAGTATAACAGCGGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAGTTTGATCAGTATATTAAAATTCAGGTACTTCTAAACTCCTCGGAGTTTCTAGGACTTATTCCTCGTCTGGATCTTTACAAGAGCACATGGGGATAAAACTAGTATAGTTTATGATGAGGTTTCCTGGAAGATGTTTCTGGTCAAAGTCTAATTGGTTTATGAACTATGTATTACTATTTATAGCATTAACTTCACACGATGCCTGCCAAATGGTGCTCATGTTGAAGTATTCTTCAATGCGGTTGATTCAATTGATCTGTGGTTTTAAAATTTTCATATGCCTTACAAACAGGAAGAACATTTGGCAAAGGGTATAAGGGACATGAAGCAGAGACATACGGCCTCGTTTCTTAATGCAATAGAGAAAGGGATCGGGAGGAAACTACATGAGAAGGATGTTGAAATTGAGAATATGAACCGTAAAAACAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCGACGGAAGCCCAGAATTGGCATAGCAGAGCTAAATACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTCCAGCATGCTATCTCGCAGGGTGGGGACCAAGCAAAGGAAGGATTCGGAGACAGTGAGGTGGATGATGCTGCCTCACACGTTGATCCAAACAATTACCTTAGTGTTCCAGGAGGGAATGTGAAATCTTGTACCAGGAATTTACCAGGCTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTCATGCCTTGTAGACACCTTTGTTTGTGTAAAGATTGTGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGCATTCAAGTGTATTTGTCCTAA

mRNA sequence

ATGTTGGGTGGTGACAATGGCAATGCCTTGCTTCCCATTTTCCTGGATGAAAATCGAACTCAGTATCAAAGTAATGCTTCTGGTCAGCTGCAGCTATTTGGAAATTTTCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAGTGAGCAAATGGCTCATATGCTCCGCCCTAACAAAAGAAGTAGGGAAGTAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAACGCCTGTCCAGATGATGCTGATCGTTCAGCAAACATTAGAAACCCAAATGCCGTGTCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACTACTGCAAGTGGAAGTATAACAGCGGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAGTTTGATCAGTATATTAAAATTCAGGAAGAACATTTGGCAAAGGGTATAAGGGACATGAAGCAGAGACATACGGCCTCGTTTCTTAATGCAATAGAGAAAGGGATCGGGAGGAAACTACATGAGAAGGATGTTGAAATTGAGAATATGAACCGTAAAAACAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCGACGGAAGCCCAGAATTGGCATAGCAGAGCTAAATACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTCCAGCATGCTATCTCGCAGGGTGGGGACCAAGCAAAGGAAGGATTCGGAGACAGTGAGGTGGATGATGCTGCCTCACACGTTGATCCAAACAATTACCTTAGTGTTCCAGGAGGGAATGTGAAATCTTGTACCAGGAATTTACCAGGCTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTCATGCCTTGTAGACACCTTTGTTTGTGTAAAGATTGTGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGCATTCAAGTGTATTTGTCCTAA

Coding sequence (CDS)

ATGTTGGGTGGTGACAATGGCAATGCCTTGCTTCCCATTTTCCTGGATGAAAATCGAACTCAGTATCAAAGTAATGCTTCTGGTCAGCTGCAGCTATTTGGAAATTTTCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAGTGAGCAAATGGCTCATATGCTCCGCCCTAACAAAAGAAGTAGGGAAGTAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAACGCCTGTCCAGATGATGCTGATCGTTCAGCAAACATTAGAAACCCAAATGCCGTGTCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACTACTGCAAGTGGAAGTATAACAGCGGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAGTTTGATCAGTATATTAAAATTCAGGAAGAACATTTGGCAAAGGGTATAAGGGACATGAAGCAGAGACATACGGCCTCGTTTCTTAATGCAATAGAGAAAGGGATCGGGAGGAAACTACATGAGAAGGATGTTGAAATTGAGAATATGAACCGTAAAAACAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCGACGGAAGCCCAGAATTGGCATAGCAGAGCTAAATACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTCCAGCATGCTATCTCGCAGGGTGGGGACCAAGCAAAGGAAGGATTCGGAGACAGTGAGGTGGATGATGCTGCCTCACACGTTGATCCAAACAATTACCTTAGTGTTCCAGGAGGGAATGTGAAATCTTGTACCAGGAATTTACCAGGCTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTCATGCCTTGTAGACACCTTTGTTTGTGTAAAGATTGTGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGCATTCAAGTGTATTTGTCCTAA

Protein sequence

MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPNKRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAISQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Homology
BLAST of Moc08g00270 vs. NCBI nr
Match: XP_022132217.1 (BOI-related E3 ubiquitin-protein ligase 1 [Momordica charantia])

HSP 1 Score: 680.2 bits (1754), Expect = 8.8e-192
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN
Sbjct: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE
Sbjct: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV
Sbjct: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. NCBI nr
Match: XP_022997618.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.1 BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_023545645.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545646.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545647.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.2 bits (1611), Expect = 3.4e-175
Identity = 309/339 (91.15%), Postives = 325/339 (95.87%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+ AAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. NCBI nr
Match: XP_022961638.1 (BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961639.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961640.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata])

HSP 1 Score: 624.0 bits (1608), Expect = 7.5e-175
Identity = 308/339 (90.86%), Postives = 324/339 (95.58%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHVASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. NCBI nr
Match: KAG6598221.1 (putative BOI-related E3 ubiquitin-protein ligase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 623.6 bits (1607), Expect = 9.8e-175
Identity = 308/339 (90.86%), Postives = 324/339 (95.58%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. NCBI nr
Match: XP_038884634.1 (probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida])

HSP 1 Score: 623.6 bits (1607), Expect = 9.8e-175
Identity = 309/339 (91.15%), Postives = 325/339 (95.87%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+FLDENRTQYQ+NASGQLQLFGN PVSCGIEPVNYFG+EQ+ HMLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQIVHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFSRQQKLQISLNY AC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLNY-ACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+TAAPSII SLGDNIRTEIDRQKEEFDQYIK+QEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMTAAPSIILSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIK VATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAAS++DPNNYLSVPGG +KS T NLPGFKEHMICRACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKSSTSNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVC LIKTAGIQV+LS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVFLS 338

BLAST of Moc08g00270 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.3e-22
Identity = 74/244 (30.33%), Postives = 123/244 (50.41%), Query Frame = 0

Query: 115 SSVTTASGSITAAPSIIFSLGD--NIRTEIDRQKEEFDQYIKI---QEEHLAKGIRDMKQ 174
           SS    + + T   ++   LG   +I + +++Q+ E D+++ +   Q E +   I + ++
Sbjct: 120 SSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRK 179

Query: 175 RHTASFLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVV 234
           R   + + AIE+G+ ++L  K+ E E + + N  L ER+K ++ E Q W   A+ NE+  
Sbjct: 180 RQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATA 239

Query: 235 NVLKNNLQHAISQGGDQAKEGFG----DSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPG 294
           N L+ NL+H ++Q  D ++ G G     +E DDA S        S  GG  +   R   G
Sbjct: 240 NHLRTNLEHVLAQVKDVSR-GAGLEKNMNEEDDAESCCG-----SSCGGGGEETVRRRVG 299

Query: 295 F----------KEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQ 340
                      +   +CR C  +E  +LL+PCRHLCLC  C   V+ CP+C   K A + 
Sbjct: 300 LEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVH 357

BLAST of Moc08g00270 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.5e-19
Identity = 62/217 (28.57%), Postives = 104/217 (47.93%), Query Frame = 0

Query: 134 LGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIEKGIGRKLHEKDVE 193
           LG ++ + + +   + D+ I    E +   I + ++      + A+E+G+ + L  KD E
Sbjct: 126 LGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDE 185

Query: 194 IENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAIS-----------Q 253
           I ++ + N  L E++K +  E Q W   A+ NE+ VN L++NLQ  ++            
Sbjct: 186 INHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEPPT 245

Query: 254 GGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEVSI 313
             D A+   G +  D+  S  +            K C   +       +CR+C   E S+
Sbjct: 246 VADDAQSCCGSN--DEGDSEEERWKLAGEAQDTKKMCRVGM------SMCRSCGKGEASV 305

Query: 314 LLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           LL+PCRH+CLC  C   +N CP+C+  KTA + V LS
Sbjct: 306 LLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

BLAST of Moc08g00270 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 9.3e-19
Identity = 66/203 (32.51%), Postives = 100/203 (49.26%), Query Frame = 0

Query: 140 TEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIEKGIGRKLHEKDVEIENMNR 199
           ++I +Q  E D+++  Q E L   +   ++  T    +A++  I +KL  KD EI  M +
Sbjct: 104 SQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGK 163

Query: 200 KNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAISQGGD-QAKEGFGDSEVDD 259
            N  L ER+K +  E Q W   A+ NE+  N L++NL+  ++Q  D  A       E DD
Sbjct: 164 LNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADD 223

Query: 260 AASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEVSILLMPCRHLCLCKDC-- 319
           A S        S  GG+V +             C+ C     S+L++PCRHLCLC  C  
Sbjct: 224 AESSCG-----SCDGGDVTAVVNG--------GCKRCGELTASVLVLPCRHLCLCTVCGS 283

Query: 320 DGLVNVCPVCQLIKTAGIQVYLS 340
             L+  CPVC ++ TA + V +S
Sbjct: 284 SALLRTCPVCDMVMTASVHVNMS 293

BLAST of Moc08g00270 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.7e-18
Identity = 82/274 (29.93%), Postives = 130/274 (47.45%), Query Frame = 0

Query: 73  QKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTASGSI-TAAPSII 132
           + L    N+N    ++  S N   P  +ST  +  +   + N+ V   S +  +++PS+I
Sbjct: 51  EPLAAKANFNKA--ESGLSYNFTVP-PLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLI 110

Query: 133 -FSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIEKGIGRKLHEK 192
              L   I+   ++Q+ E D+++  Q E L   I   +Q  T    +A++  I +KL EK
Sbjct: 111 NVELVSQIQ---NQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEK 170

Query: 193 DVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAISQGGDQAKEG 252
           D EI  +   N  L ER+K +  E Q W   A+ NE+  N L+ NL   ++Q        
Sbjct: 171 DDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQ-------- 230

Query: 253 FGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMI---CRACKAKEVSILLMPC 312
               E    AS V  ++  S  G    SC  +  G     +   C+ C  +E S+L++PC
Sbjct: 231 ---LETFPTASAVVEDDAESSCG----SCCGDGGGEAVTAVGGGCKRCGEREASVLVLPC 290

Query: 313 RHLCLCKDCDG--LVNVCPVCQLIKTAGIQVYLS 340
           RHLCLC  C G  L+  CPVC ++  A + V +S
Sbjct: 291 RHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

BLAST of Moc08g00270 vs. ExPASy Swiss-Prot
Match: O62640 (Putative inhibitor of apoptosis OS=Sus scrofa OX=9823 GN=PIAP PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.1e-06
Identity = 26/71 (36.62%), Postives = 38/71 (53.52%), Query Frame = 0

Query: 271 VPGGNVK--SCTRNLPGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQL 330
           +P  NV   S    L   +E   C+ C  KEVSI+ +PC HL +CKDC   +  CP+C+ 
Sbjct: 288 IPTENVSDLSMEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRG 347

Query: 331 IKTAGIQVYLS 340
                ++ +LS
Sbjct: 348 TIKGTVRTFLS 358

BLAST of Moc08g00270 vs. ExPASy TrEMBL
Match: A0A6J1BT85 (BOI-related E3 ubiquitin-protein ligase 1 OS=Momordica charantia OX=3673 GN=LOC111005133 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 4.3e-192
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN
Sbjct: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE
Sbjct: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV
Sbjct: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. ExPASy TrEMBL
Match: A0A6J1KC06 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492498 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.6e-175
Identity = 309/339 (91.15%), Postives = 325/339 (95.87%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+ AAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. ExPASy TrEMBL
Match: A0A6J1HCD7 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC111462352 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.6e-175
Identity = 308/339 (90.86%), Postives = 324/339 (95.58%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGG+NGN LLP+F DENR QYQ+NASGQLQLFG+ PVSCGIEPVNYFGSEQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFS QQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHVASFLSAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIE+MNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYL+V GG +KS T NLPGFKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. ExPASy TrEMBL
Match: A0A6J1GH18 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454110 PE=4 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 304/339 (89.68%), Postives = 321/339 (94.69%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGGDNGNA+LP+FLDENRTQYQ+NASGQLQLFGN PVSCGIEPVNYFG+EQMAHMLRPN
Sbjct: 1   MLGGDNGNAMLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFSRQQKLQISL+YNAC DDADR A+I NPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLDYNACQDDADRPASIINPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+TAAPSIIFSLGDN+R EIDRQKEE DQYIKIQEEHLAKGIR+MKQRH ASF+NAIE
Sbjct: 121 SGSMTAAPSIIFSLGDNLRIEIDRQKEELDQYIKIQEEHLAKGIREMKQRHMASFVNAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIENMN KNRELVERIKQV TEAQNW  RAKYNESVVNVLK+NLQH I
Sbjct: 181 KGIGKKLHEKDVEIENMNCKNRELVERIKQVGTEAQNWQCRAKYNESVVNVLKSNLQHVI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASHVD NNYLSVPGG ++S TRNL GFKE MICRACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHVDSNNYLSVPGGTMRSTTRNLAGFKERMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDC+GLV+VCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCNGLVSVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. ExPASy TrEMBL
Match: A0A6J1KPW7 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496531 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 301/339 (88.79%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60
           MLGGDNGNA+LP+FLDENRTQYQ+NASGQLQLFGN PVSCGIEPVNYFG+EQMAHMLRPN
Sbjct: 1   MLGGDNGNAMLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60

Query: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFSRQQKLQISL+YNAC DDADR A+I NPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLDYNACQDDADRPASIINPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180
           SGS+TAAPSIIFSLGDN+R EIDRQKEE DQYIKIQEEHLAKGIR+MKQRH ASF+NAIE
Sbjct: 121 SGSMTAAPSIIFSLGDNLRIEIDRQKEELDQYIKIQEEHLAKGIREMKQRHMASFVNAIE 180

Query: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKDVEIENMN KNRELVERIKQV TEAQNW  RAKYNESVVNVLK+NLQH I
Sbjct: 181 KGIGKKLHEKDVEIENMNCKNRELVERIKQVGTEAQNWQCRAKYNESVVNVLKSNLQHVI 240

Query: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+D NNYLSVPGG ++S TRNL GFKE MICRACK KEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDSNNYLSVPGGTMRSSTRNLAGFKERMICRACKEKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDC+GL +VCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCNGLASVCPVCQLIKTAGIQVYLS 339

BLAST of Moc08g00270 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.2e-103
Identity = 198/352 (56.25%), Postives = 247/352 (70.17%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSN-ASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRP 60
           ML G+NGN   P+F++EN+ QYQ+N  S QL L GN    C ++PVNYF ++ +  M+RP
Sbjct: 1   MLSGNNGNTAPPVFMNENQLQYQTNLRSNQLHLLGNMGGGCTVDPVNYFANDNLVPMIRP 60

Query: 61  NKRSREVEDFS-----RQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHN 120
           NKR RE E  S     +QQKLQ+SLNYN   ++      +   N VSTGLRLSYDDDEHN
Sbjct: 61  NKRGREAESISNNIQRQQQKLQMSLNYNY--NNTSVREEVPKENLVSTGLRLSYDDDEHN 120

Query: 121 SSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTAS 180
           SSVT+ASGSI AA  I  SL D++R ++ RQK+EFDQ+IKIQ   +AKG+RDMKQRH AS
Sbjct: 121 SSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRHIAS 180

Query: 181 FLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKN 240
           FL  +EKG+ +KL EKD EI +MN+KN+ELVERIKQVA EAQNWH RAKYNESVVNVLK 
Sbjct: 181 FLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNVLKA 240

Query: 241 NLQHAISQ------GGDQAKEGFGDSEVDDAA-SHVDPNNYLSVPGGNVKSCTRNLPGFK 300
           NLQ A+S         DQ KEGFGDSE+DDAA S++DPNN              N  G  
Sbjct: 241 NLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNNN-----------NNNNMGIH 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           + M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC+ +K++ +QV+ S
Sbjct: 301 QRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 339

BLAST of Moc08g00270 vs. TAIR 10
Match: AT1G60610.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.2e-92
Identity = 185/354 (52.26%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNAS-GQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRP 60
           MLGG+N N +  + +++++ +YQSN S  QL L G     C I+PVNYF ++ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREVED---FSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH  SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNN 240
           + A+EK + +KL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGGD------------QAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG         +
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---------M 300

Query: 301 PGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
           P     M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 P--STGMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of Moc08g00270 vs. TAIR 10
Match: AT1G60610.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.2e-92
Identity = 185/354 (52.26%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNAS-GQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRP 60
           MLGG+N N +  + +++++ +YQSN S  QL L G     C I+PVNYF ++ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREVED---FSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH  SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNN 240
           + A+EK + +KL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGGD------------QAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG         +
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---------M 300

Query: 301 PGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
           P     M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 P--STGMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of Moc08g00270 vs. TAIR 10
Match: AT1G60610.3 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.2e-92
Identity = 185/354 (52.26%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 1   MLGGDNGNALLPIFLDENRTQYQSNAS-GQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRP 60
           MLGG+N N +  + +++++ +YQSN S  QL L G     C I+PVNYF ++ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREVED---FSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH  SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNN 240
           + A+EK + +KL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGGD------------QAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG         +
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---------M 300

Query: 301 PGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
           P     M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 P--STGMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of Moc08g00270 vs. TAIR 10
Match: AT1G10650.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.8e-86
Identity = 172/296 (58.11%), Postives = 210/296 (70.95%), Query Frame = 0

Query: 56  MLRPNKRSREVEDFS-----RQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDD 115
           M+RPNKR RE E  S     +QQKLQ+SLNYN   ++      +   N VSTGLRLSYDD
Sbjct: 1   MIRPNKRGREAESISNNIQRQQQKLQMSLNYNY--NNTSVREEVPKENLVSTGLRLSYDD 60

Query: 116 DEHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQR 175
           DEHNSSVT+ASGSI AA  I  SL D++R ++ RQK+EFDQ+IKIQ   +AKG+RDMKQR
Sbjct: 61  DEHNSSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQR 120

Query: 176 HTASFLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVN 235
           H ASFL  +EKG+ +KL EKD EI +MN+KN+ELVERIKQVA EAQNWH RAKYNESVVN
Sbjct: 121 HIASFLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVN 180

Query: 236 VLKNNLQHAISQ------GGDQAKEGFGDSEVDDAA-SHVDPNNYLSVPGGNVKSCTRNL 295
           VLK NLQ A+S         DQ KEGFGDSE+DDAA S++DPNN              N 
Sbjct: 181 VLKANLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNNN-----------NNNN 240

Query: 296 PGFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
            G  + M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC+ +K++ +QV+ S
Sbjct: 241 MGIHQRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132217.18.8e-192100.00BOI-related E3 ubiquitin-protein ligase 1 [Momordica charantia][more]
XP_022997618.13.4e-17591.15BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.... [more]
XP_022961638.17.5e-17590.86BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961639.1 B... [more]
KAG6598221.19.8e-17590.86putative BOI-related E3 ubiquitin-protein ligase 2, partial [Cucurbita argyrospe... [more]
XP_038884634.19.8e-17591.15probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
F4IDI65.3e-2230.33Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
Q9LDD12.5e-1928.57Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
Q9FHE49.3e-1932.51BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
O818512.7e-1829.93E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
O626404.1e-0636.62Putative inhibitor of apoptosis OS=Sus scrofa OX=9823 GN=PIAP PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BT854.3e-192100.00BOI-related E3 ubiquitin-protein ligase 1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1KC061.6e-17591.15BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HCD73.6e-17590.86BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1GH181.1e-17189.68probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita mosch... [more]
A0A6J1KPW71.6e-17088.79probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
AT1G10650.12.2e-10356.25SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.12.2e-9252.26SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.22.2e-9252.26SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.32.2e-9252.26SBP (S-ribonuclease binding protein) family protein [more]
AT1G10650.21.8e-8658.11SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 187..214
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 290..327
e-value: 4.8E-7
score: 29.5
NoneNo IPR availablePANTHERPTHR42647:SF9S-RIBONUCLEASE BINDING PROTEIN SBP1-RELATEDcoord: 4..338
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 277..339
e-value: 4.0E-8
score: 34.6
IPR017066E3 ubiquitin-protein ligase BOI-likePIRSFPIRSF036836SBP1_RNase_bindcoord: 1..339
e-value: 4.8E-98
score: 326.9
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 4..338
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 292..327
score: 8.963311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g00270.1Moc08g00270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity