Moc07g00370.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g00370.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	CCHC-type domain-containing protein
Location	chr7: 196856 .. 197448 (+)
Sequence length	471
RNA-Seq Expression	Moc07g00370.1
Synteny	Moc07g00370.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGACAAATATCACGTACACCATTACTACGAAGGGATTCTGAATTTGAGATTTTGGAAGATGCAGGTAAAGGATCTTCTTACGTGCAAGAAGATACACAAGACTTTGGGGCATAGACCAGCGGAGATGACCGACAAGGATTAGAAAGGGTAGATGAGCAGGTCGTTGCAAACATCAGAATATCTTTATCGATAAGGGTATGCAGTCTGGTAGCGAAAGAGACAACAACGAAAGGACTGTTGAAGGCCTTGCAAGATAAGTATGAGAAACCTTTTGCCAACACAAAAATACTTCTATGGACGAAGTATTTCAATATCCACATAGAGCAGGGAACTTCAATGAATTCTCACATTAATGAGCTAACCGATATATTGAACAAACTGGAAGGGATGTGCATCAAAATCGATGAGGAGGTGAAGCCTATGAGGTTGCTGACATCTTTGCCTGGCAGTTGGGAGACGATGAAGACTGCAGTGTCGAATTCGTTAAGGGACAATAGTCTGAAATTTTCAGCTATTTGTGATGCTGCTTTATCTAAGGAAACCCGAAGAAAATTGGAGAAAATGTCTGTATCTACTTCAGGGGCATAA

mRNA sequence

ATGAGAGACAAATATCACGTACACCATTACTACGAAGGGATTCTGAATTTGAGATTTTGGAAGATGCAGGTAAAGGATCTTCTTACTCTGGTAGCGAAAGAGACAACAACGAAAGGACTGTTGAAGGCCTTGCAAGATAAGTATGAGAAACCTTTTGCCAACACAAAAATACTTCTATGGACGAAGTATTTCAATATCCACATAGAGCAGGGAACTTCAATGAATTCTCACATTAATGAGCTAACCGATATATTGAACAAACTGGAAGGGATGTGCATCAAAATCGATGAGGAGGTGAAGCCTATGAGGTTGCTGACATCTTTGCCTGGCAGTTGGGAGACGATGAAGACTGCAGTGTCGAATTCGTTAAGGGACAATAGTCTGAAATTTTCAGCTATTTGTGATGCTGCTTTATCTAAGGAAACCCGAAGAAAATTGGAGAAAATGTCTGTATCTACTTCAGGGGCATAA

Coding sequence (CDS)

ATGAGAGACAAATATCACGTACACCATTACTACGAAGGGATTCTGAATTTGAGATTTTGGAAGATGCAGGTAAAGGATCTTCTTACTCTGGTAGCGAAAGAGACAACAACGAAAGGACTGTTGAAGGCCTTGCAAGATAAGTATGAGAAACCTTTTGCCAACACAAAAATACTTCTATGGACGAAGTATTTCAATATCCACATAGAGCAGGGAACTTCAATGAATTCTCACATTAATGAGCTAACCGATATATTGAACAAACTGGAAGGGATGTGCATCAAAATCGATGAGGAGGTGAAGCCTATGAGGTTGCTGACATCTTTGCCTGGCAGTTGGGAGACGATGAAGACTGCAGTGTCGAATTCGTTAAGGGACAATAGTCTGAAATTTTCAGCTATTTGTGATGCTGCTTTATCTAAGGAAACCCGAAGAAAATTGGAGAAAATGTCTGTATCTACTTCAGGGGCATAA

Protein sequence

MRDKYHVHHYYEGILNLRFWKMQVKDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDILNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRRKLEKMSVSTSGA

Homology

[Close]

BLAST of Moc07g00370.1 vs. NCBI nr
Match: KAG7593230.1 (Pentatricopeptide repeat [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 111.3 bits (277), Expect = 7.5e-21
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 68  KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + LL SLP SWE M+ AVSNS+ +  LKF  + D  L +E RR
Sbjct: 128 VNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 188 -IDTGETSTSSA 198

[Close]

BLAST of Moc07g00370.1 vs. NCBI nr
Match: KAG7584790.1 (Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 110.5 bits (275), Expect = 1.3e-20
Identity = 56/120 (46.67%), Postives = 80/120 (66.67%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 68  KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + LL SLP SWE M+ AVSNS+ +  LKF  + D  L +E RR
Sbjct: 128 VNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

[Close]

BLAST of Moc07g00370.1 vs. NCBI nr
Match: KAG7561662.1 (Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 110.5 bits (275), Expect = 1.3e-20
Identity = 59/132 (44.70%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 68  KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + L+ SLP SWE M+ AVSNS+ +  LKF  + D  L +E RR
Sbjct: 128 VNQLSSVEIEFDDEVRALILIASLPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 188 -IDTGETSTSSA 198

[Close]

BLAST of Moc07g00370.1 vs. NCBI nr
Match: VDD56318.1 (unnamed protein product [Brassica oleracea])

HSP 1 Score: 108.6 bits (270), Expect = 4.9e-20
Identity = 58/132 (43.94%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 417 KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGCLVAAHVNEFNTI 476

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ ++EV+ + LL SLP SWE M+ AVSN +    LKF+ + D  L++E RR
Sbjct: 477 VNQLSSVEIEFEDEVRALILLASLPNSWEPMRAAVSNFVGTQKLKFNDVKDRILAEEVRR 536

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 537 -IDSGEASTSSA 547

[Close]

BLAST of Moc07g00370.1 vs. NCBI nr
Match: KAE8735860.1 (Cytochrome P450 90B1 [Hibiscus syriacus])

HSP 1 Score: 107.5 bits (267), Expect = 1.1e-19
Identity = 63/151 (41.72%), Postives = 88/151 (58.28%), Query Frame = 0


Query: 19  FWKMQVKDLLTL-------------VAKETTTKGLLKALQDKYEKPFANTKILLWTKYFN 78
           FWKMQ++D L               +AKE TT GL+ AL   YEKP A+ K+ L  + FN
Sbjct: 373 FWKMQIEDFLPRSHPTDVVPKCCFHIAKEKTTAGLMAALSSMYEKPSASNKVHLMRRLFN 432

Query: 79  IHIEQGTSMNSHINELTDILNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRD 138
           + + +G S+  H+NEL  I  +L  + I+ D+EV+ + LL+SLP SW    TAVS+S  +
Sbjct: 433 LRMTEGASVAQHLNELNTITTQLSSVEIEFDDEVRALILLSSLPDSWNATVTAVSSSSGN 492

Query: 139 NSLKFSAICDAALSKETRRKLEKMSVSTSGA 157
           + LKF  + D  LS+E RR+ E    STS A
Sbjct: 493 SKLKFDDVRDLVLSEEIRRR-ESGEASTSSA 522

[Close]

BLAST of Moc07g00370.1 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 4.5e-08
Identity = 32/120 (26.67%), Postives = 62/120 (51.67%), Query Frame = 0


Query: 26  DLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDIL 85
           D++  +  E T +G+   L+  Y       K+ L  + + +H+ +GT+  SH+N    ++
Sbjct: 70  DVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQLYALHMSEGTNFLSHLNVFNGLI 129

Query: 86  NKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRRK 145
            +L  + +KI+EE K + LL SLP S++ + T + +     +++   +  A L  E  RK
Sbjct: 130 TQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILHG--KTTIELKDVTSALLLNEKMRK 187

[Close]

BLAST of Moc07g00370.1 vs. ExPASy TrEMBL
Match: A0A0D3BM55 (Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.1e-21
Identity = 60/132 (45.45%), Postives = 87/132 (65.91%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 45  KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGLVAAHVNEFNTI 104

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + LL SLP SWE M+ AVSNS+    LKF+ + D  L++E RR
Sbjct: 105 VNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVSNSVGTQKLKFNDVRDRILAEEVRR 164

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 165 -IDSGEASTSSA 175

[Close]

BLAST of Moc07g00370.1 vs. ExPASy TrEMBL
Match: A0A0D3AEM1 (CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 3.7e-21
Identity = 59/132 (44.70%), Postives = 88/132 (66.67%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   V KE TT+GL+K L D YEKP AN+K+ L  K F++ +E+G  + +HINE   I
Sbjct: 68  KNVAHNVVKEKTTEGLMKVLSDMYEKPSANSKVFLMKKLFHLKMEEGGLVTAHINEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ ++EV+ + LL SLP SWE+M+ AVSNS+    LKF+ + D  L++E RR
Sbjct: 128 VNQLSSVEIEFEDEVRALILLASLPNSWESMRVAVSNSVGTQKLKFNDVRDRILAEEVRR 187

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 188 -IDSGEASTSSA 198

[Close]

BLAST of Moc07g00370.1 vs. ExPASy TrEMBL
Match: A0A0D3DMW7 (CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.8e-20
Identity = 57/132 (43.18%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   +AKE TT+GL+K L D YEKP  N K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 69  KNVTHNIAKEKTTEGLMKVLSDMYEKPSVNNKVFLMKKLFHLKMEEGGLVAAHVNEFNTI 128

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + LL SLP SWE M+ AV+NS+    LKF+ + D  L++E RR
Sbjct: 129 VNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVNNSVGTQKLKFNDVRDRILAEEVRR 188

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 189 -IDSGETSTSSA 199

[Close]

BLAST of Moc07g00370.1 vs. ExPASy TrEMBL
Match: A0A3P6FTI2 (Abhydrolase_3 domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC8T49547H PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.4e-20
Identity = 58/132 (43.94%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 417 KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGCLVAAHVNEFNTI 476

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ ++EV+ + LL SLP SWE M+ AVSN +    LKF+ + D  L++E RR
Sbjct: 477 VNQLSSVEIEFEDEVRALILLASLPNSWEPMRAAVSNFVGTQKLKFNDVKDRILAEEVRR 536

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 537 -IDSGEASTSSA 547

[Close]

BLAST of Moc07g00370.1 vs. ExPASy TrEMBL
Match: A0A0D3CS45 (Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 3.1e-20
Identity = 58/132 (43.94%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE  T+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 68  KNVAHNVAKEKITEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGLVAAHVNEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ ++EV+ + LL SLP SWE M+ AVSNS+    LKF+ + D  L++E RR
Sbjct: 128 VNQLSSVEIEFEDEVRALILLASLPNSWEPMRAAVSNSVGTQKLKFNDVRDRILAEEVRR 187

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 188 -IDSGEASTSSA 198

The following BLAST results are available for this feature:

BLAST of Moc07g00370.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7593230.1	7.5e-21	45.45	Pentatricopeptide repeat [Arabidopsis thaliana x Arabidopsis arenosa]	[more]
KAG7584790.1	1.3e-20	46.67	Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa]	[more]
KAG7561662.1	1.3e-20	44.70	Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa]	[more]
VDD56318.1	4.9e-20	43.94	unnamed protein product [Brassica oleracea]	[more]
KAE8735860.1	1.1e-19	41.72	Cytochrome P450 90B1 [Hibiscus syriacus]	[more]

BLAST of Moc07g00370.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	4.5e-08	26.67	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]

BLAST of Moc07g00370.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0D3BM55	2.1e-21	45.45	Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1	[more]
A0A0D3AEM1	3.7e-21	44.70	CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376...	[more]
A0A0D3DMW7	1.8e-20	43.18	CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376...	[more]
A0A3P6FTI2	2.4e-20	43.94	Abhydrolase_3 domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC8T49...	[more]
A0A0D3CS45	3.1e-20	43.94	Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1	[more]

BLAST of Moc07g00370.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF14223	Retrotran_gag_2	coord: 26..145 e-value: 2.3E-21 score: 76.0
None	No IPR available	PANTHER	PTHR34676	FAMILY NOT NAMED	coord: 32..145
None	No IPR available	PANTHER	PTHR34676:SF1	ZINC FINGER, CCHC-TYPE, TUBBY C-TERMINAL-LIKE DOMAIN PROTEIN-RELATED	coord: 32..145

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc07g00370	Moc07g00370	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc07g00370.1.exon1	Moc07g00370.1.exon1	exon
Moc07g00370.1.exon2	Moc07g00370.1.exon2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc07g00370.1	cds.Moc07g00370.1	CDS
cds.Moc07g00370.1	cds.Moc07g00370.1_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc07g00370.1	Moc07g00370.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044260	cellular macromolecule metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0044238	primary metabolic process
molecular_function	GO:1901363	heterocyclic compound binding
molecular_function	GO:0043167	ion binding
molecular_function	GO:0097159	organic cyclic compound binding
molecular_function	GO:0016772	transferase activity, transferring phosphorus-containing groups