Moc07g00370 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc07g00370
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCCHC-type domain-containing protein
Locationchr7: 196856 .. 197448 (+)
RNA-Seq ExpressionMoc07g00370
SyntenyMoc07g00370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGACAAATATCACGTACACCATTACTACGAAGGGATTCTGAATTTGAGATTTTGGAAGATGCAGGTAAAGGATCTTCTTACGTGCAAGAAGATACACAAGACTTTGGGGCATAGACCAGCGGAGATGACCGACAAGGATTAGAAAGGGTAGATGAGCAGGTCGTTGCAAACATCAGAATATCTTTATCGATAAGGGTATGCAGTCTGGTAGCGAAAGAGACAACAACGAAAGGACTGTTGAAGGCCTTGCAAGATAAGTATGAGAAACCTTTTGCCAACACAAAAATACTTCTATGGACGAAGTATTTCAATATCCACATAGAGCAGGGAACTTCAATGAATTCTCACATTAATGAGCTAACCGATATATTGAACAAACTGGAAGGGATGTGCATCAAAATCGATGAGGAGGTGAAGCCTATGAGGTTGCTGACATCTTTGCCTGGCAGTTGGGAGACGATGAAGACTGCAGTGTCGAATTCGTTAAGGGACAATAGTCTGAAATTTTCAGCTATTTGTGATGCTGCTTTATCTAAGGAAACCCGAAGAAAATTGGAGAAAATGTCTGTATCTACTTCAGGGGCATAA

mRNA sequence

ATGAGAGACAAATATCACGTACACCATTACTACGAAGGGATTCTGAATTTGAGATTTTGGAAGATGCAGGTAAAGGATCTTCTTACTCTGGTAGCGAAAGAGACAACAACGAAAGGACTGTTGAAGGCCTTGCAAGATAAGTATGAGAAACCTTTTGCCAACACAAAAATACTTCTATGGACGAAGTATTTCAATATCCACATAGAGCAGGGAACTTCAATGAATTCTCACATTAATGAGCTAACCGATATATTGAACAAACTGGAAGGGATGTGCATCAAAATCGATGAGGAGGTGAAGCCTATGAGGTTGCTGACATCTTTGCCTGGCAGTTGGGAGACGATGAAGACTGCAGTGTCGAATTCGTTAAGGGACAATAGTCTGAAATTTTCAGCTATTTGTGATGCTGCTTTATCTAAGGAAACCCGAAGAAAATTGGAGAAAATGTCTGTATCTACTTCAGGGGCATAA

Coding sequence (CDS)

ATGAGAGACAAATATCACGTACACCATTACTACGAAGGGATTCTGAATTTGAGATTTTGGAAGATGCAGGTAAAGGATCTTCTTACTCTGGTAGCGAAAGAGACAACAACGAAAGGACTGTTGAAGGCCTTGCAAGATAAGTATGAGAAACCTTTTGCCAACACAAAAATACTTCTATGGACGAAGTATTTCAATATCCACATAGAGCAGGGAACTTCAATGAATTCTCACATTAATGAGCTAACCGATATATTGAACAAACTGGAAGGGATGTGCATCAAAATCGATGAGGAGGTGAAGCCTATGAGGTTGCTGACATCTTTGCCTGGCAGTTGGGAGACGATGAAGACTGCAGTGTCGAATTCGTTAAGGGACAATAGTCTGAAATTTTCAGCTATTTGTGATGCTGCTTTATCTAAGGAAACCCGAAGAAAATTGGAGAAAATGTCTGTATCTACTTCAGGGGCATAA

Protein sequence

MRDKYHVHHYYEGILNLRFWKMQVKDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDILNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRRKLEKMSVSTSGA
Homology
BLAST of Moc07g00370 vs. NCBI nr
Match: KAG7593230.1 (Pentatricopeptide repeat [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 111.3 bits (277), Expect = 7.5e-21
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 68  KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + LL SLP SWE M+ AVSNS+ +  LKF  + D  L +E RR
Sbjct: 128 VNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 188 -IDTGETSTSSA 198

BLAST of Moc07g00370 vs. NCBI nr
Match: KAG7584790.1 (Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 110.5 bits (275), Expect = 1.3e-20
Identity = 56/120 (46.67%), Postives = 80/120 (66.67%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 68  KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + LL SLP SWE M+ AVSNS+ +  LKF  + D  L +E RR
Sbjct: 128 VNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

BLAST of Moc07g00370 vs. NCBI nr
Match: KAG7561662.1 (Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 110.5 bits (275), Expect = 1.3e-20
Identity = 59/132 (44.70%), Postives = 86/132 (65.15%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 68  KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + L+ SLP SWE M+ AVSNS+ +  LKF  + D  L +E RR
Sbjct: 128 VNQLSSVEIEFDDEVRALILIASLPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 188 -IDTGETSTSSA 198

BLAST of Moc07g00370 vs. NCBI nr
Match: VDD56318.1 (unnamed protein product [Brassica oleracea])

HSP 1 Score: 108.6 bits (270), Expect = 4.9e-20
Identity = 58/132 (43.94%), Postives = 86/132 (65.15%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 417 KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGCLVAAHVNEFNTI 476

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ ++EV+ + LL SLP SWE M+ AVSN +    LKF+ + D  L++E RR
Sbjct: 477 VNQLSSVEIEFEDEVRALILLASLPNSWEPMRAAVSNFVGTQKLKFNDVKDRILAEEVRR 536

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 537 -IDSGEASTSSA 547

BLAST of Moc07g00370 vs. NCBI nr
Match: KAE8735860.1 (Cytochrome P450 90B1 [Hibiscus syriacus])

HSP 1 Score: 107.5 bits (267), Expect = 1.1e-19
Identity = 63/151 (41.72%), Postives = 88/151 (58.28%), Query Frame = 0

Query: 19  FWKMQVKDLLTL-------------VAKETTTKGLLKALQDKYEKPFANTKILLWTKYFN 78
           FWKMQ++D L               +AKE TT GL+ AL   YEKP A+ K+ L  + FN
Sbjct: 373 FWKMQIEDFLPRSHPTDVVPKCCFHIAKEKTTAGLMAALSSMYEKPSASNKVHLMRRLFN 432

Query: 79  IHIEQGTSMNSHINELTDILNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRD 138
           + + +G S+  H+NEL  I  +L  + I+ D+EV+ + LL+SLP SW    TAVS+S  +
Sbjct: 433 LRMTEGASVAQHLNELNTITTQLSSVEIEFDDEVRALILLSSLPDSWNATVTAVSSSSGN 492

Query: 139 NSLKFSAICDAALSKETRRKLEKMSVSTSGA 157
           + LKF  + D  LS+E RR+ E    STS A
Sbjct: 493 SKLKFDDVRDLVLSEEIRRR-ESGEASTSSA 522

BLAST of Moc07g00370 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 4.5e-08
Identity = 32/120 (26.67%), Postives = 62/120 (51.67%), Query Frame = 0

Query: 26  DLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDIL 85
           D++  +  E T +G+   L+  Y       K+ L  + + +H+ +GT+  SH+N    ++
Sbjct: 70  DVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQLYALHMSEGTNFLSHLNVFNGLI 129

Query: 86  NKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRRK 145
            +L  + +KI+EE K + LL SLP S++ + T + +     +++   +  A L  E  RK
Sbjct: 130 TQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILHG--KTTIELKDVTSALLLNEKMRK 187

BLAST of Moc07g00370 vs. ExPASy TrEMBL
Match: A0A0D3BM55 (Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.1e-21
Identity = 60/132 (45.45%), Postives = 87/132 (65.91%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 45  KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGLVAAHVNEFNTI 104

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + LL SLP SWE M+ AVSNS+    LKF+ + D  L++E RR
Sbjct: 105 VNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVSNSVGTQKLKFNDVRDRILAEEVRR 164

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 165 -IDSGEASTSSA 175

BLAST of Moc07g00370 vs. ExPASy TrEMBL
Match: A0A0D3AEM1 (CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 3.7e-21
Identity = 59/132 (44.70%), Postives = 88/132 (66.67%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   V KE TT+GL+K L D YEKP AN+K+ L  K F++ +E+G  + +HINE   I
Sbjct: 68  KNVAHNVVKEKTTEGLMKVLSDMYEKPSANSKVFLMKKLFHLKMEEGGLVTAHINEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ ++EV+ + LL SLP SWE+M+ AVSNS+    LKF+ + D  L++E RR
Sbjct: 128 VNQLSSVEIEFEDEVRALILLASLPNSWESMRVAVSNSVGTQKLKFNDVRDRILAEEVRR 187

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 188 -IDSGEASTSSA 198

BLAST of Moc07g00370 vs. ExPASy TrEMBL
Match: A0A0D3DMW7 (CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.8e-20
Identity = 57/132 (43.18%), Postives = 86/132 (65.15%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   +AKE TT+GL+K L D YEKP  N K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 69  KNVTHNIAKEKTTEGLMKVLSDMYEKPSVNNKVFLMKKLFHLKMEEGGLVAAHVNEFNTI 128

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ D+EV+ + LL SLP SWE M+ AV+NS+    LKF+ + D  L++E RR
Sbjct: 129 VNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVNNSVGTQKLKFNDVRDRILAEEVRR 188

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 189 -IDSGETSTSSA 199

BLAST of Moc07g00370 vs. ExPASy TrEMBL
Match: A0A3P6FTI2 (Abhydrolase_3 domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC8T49547H PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.4e-20
Identity = 58/132 (43.94%), Postives = 86/132 (65.15%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE TT+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 417 KNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGCLVAAHVNEFNTI 476

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ ++EV+ + LL SLP SWE M+ AVSN +    LKF+ + D  L++E RR
Sbjct: 477 VNQLSSVEIEFEDEVRALILLASLPNSWEPMRAAVSNFVGTQKLKFNDVKDRILAEEVRR 536

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 537 -IDSGEASTSSA 547

BLAST of Moc07g00370 vs. ExPASy TrEMBL
Match: A0A0D3CS45 (Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 3.1e-20
Identity = 58/132 (43.94%), Postives = 86/132 (65.15%), Query Frame = 0

Query: 25  KDLLTLVAKETTTKGLLKALQDKYEKPFANTKILLWTKYFNIHIEQGTSMNSHINELTDI 84
           K++   VAKE  T+GL+K L D YEKP AN K+ L  K F++ +E+G  + +H+NE   I
Sbjct: 68  KNVAHNVAKEKITEGLMKVLSDMYEKPSANNKVFLMKKLFHLKMEEGGLVAAHVNEFNTI 127

Query: 85  LNKLEGMCIKIDEEVKPMRLLTSLPGSWETMKTAVSNSLRDNSLKFSAICDAALSKETRR 144
           +N+L  + I+ ++EV+ + LL SLP SWE M+ AVSNS+    LKF+ + D  L++E RR
Sbjct: 128 VNQLSSVEIEFEDEVRALILLASLPNSWEPMRAAVSNSVGTQKLKFNDVRDRILAEEVRR 187

Query: 145 KLEKMSVSTSGA 157
            ++    STS A
Sbjct: 188 -IDSGEASTSSA 198

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7593230.17.5e-2145.45Pentatricopeptide repeat [Arabidopsis thaliana x Arabidopsis arenosa][more]
KAG7584790.11.3e-2046.67Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa][more]
KAG7561662.11.3e-2044.70Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa][more]
VDD56318.14.9e-2043.94unnamed protein product [Brassica oleracea][more]
KAE8735860.11.1e-1941.72Cytochrome P450 90B1 [Hibiscus syriacus][more]
Match NameE-valueIdentityDescription
P109784.5e-0826.67Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Match NameE-valueIdentityDescription
A0A0D3BM552.1e-2145.45Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1[more]
A0A0D3AEM13.7e-2144.70CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376... [more]
A0A0D3DMW71.8e-2043.18CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376... [more]
A0A3P6FTI22.4e-2043.94Abhydrolase_3 domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC8T49... [more]
A0A0D3CS453.1e-2043.94Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 26..145
e-value: 2.3E-21
score: 76.0
NoneNo IPR availablePANTHERPTHR34676FAMILY NOT NAMEDcoord: 32..145
NoneNo IPR availablePANTHERPTHR34676:SF1ZINC FINGER, CCHC-TYPE, TUBBY C-TERMINAL-LIKE DOMAIN PROTEIN-RELATEDcoord: 32..145

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc07g00370.1Moc07g00370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044260 cellular macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
molecular_function GO:1901363 heterocyclic compound binding
molecular_function GO:0043167 ion binding
molecular_function GO:0097159 organic cyclic compound binding
molecular_function GO:0016772 transferase activity, transferring phosphorus-containing groups