Moc05g00180.1 (mRNA) Bitter gourd (OHB3-1) v2

BLAST of Moc05g00180.1 vs. NCBI nr
Match: XP_023540226.1 (probable L-gulonolactone oxidase 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 960.3 bits (2481), Expect = 9.2e-276
Identity = 445/579 (76.86%), Postives = 516/579 (89.12%), Query Frame = 0

Query: 11  SFRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVY 70
           +FR  T   FL  L+  SE +PPEDPIKCRSP  N++CTI N+Y TFPDR+ CR   V Y
Sbjct: 6   AFRFTTFILFLVLLVSVSESTPPEDPIKCRSP--NANCTITNAYATFPDRAICRVGEVKY 65

Query: 71  PTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEA 130
           PTT+AE ISAVA ATRA+RK+KV TRFYHSIPKLACPDG DG++ISTKYLNR LKVD E 
Sbjct: 66  PTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEG 125

Query: 131 MTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYV 190
           M+ITVEGGVT+R++IE AA  GLALPH PYWWGLTIGG++STGAHGSSLWGKGSAVHDYV
Sbjct: 126 MSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYV 185

Query: 191 VEIQMVTPGGPQDGYAKLRRLLDGDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLK 250
           V I+MV+PGG QDGYAK+RRL+DGDQHLNAAKVSLGVLGIITQVTLKL+PMFKRSITY +
Sbjct: 186 VGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFE 245

Query: 251 RHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSV 310
           R D DL D+V+SFGR+YEF D+ WYPSQR VVYR+D+RVSSNTSGNGLYDFIPFRPTPS 
Sbjct: 246 RDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRRDTRVSSNTSGNGLYDFIPFRPTPSP 305

Query: 311 GLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQS 370
            L ++RASEE QE+RRDA GKC SA+++TSLLS SAYGLTNNGITFLKYPVIGY NR+QS
Sbjct: 306 ALAIVRASEEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQS 365

Query: 371 SGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCG 430
           SGSCL  +  D+RIT CPWDP IKGEFFH+  +S++L+VVK FIEDV+KL ELEPMAFCG
Sbjct: 366 SGSCL-DSVNDLRITACPWDPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCG 425

Query: 431 LELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYG 490
           LELYNGILMRYVKAS+AYLGK+ DG++FDFTYYRS+DPMSPRLFEDVLEEVEQMA+FKYG
Sbjct: 426 LELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYG 485

Query: 491 GTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDG 550
           G+PHWGKNRN+AF +A+ KY+++E FL++KNEYDP+GLFSN+WT+Q+LG++G V+IDKDG
Sbjct: 486 GSPHWGKNRNIAFLKAVGKYKNREGFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDG 545

Query: 551 CALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYLG 590
           CALEGLC+CSKDSHCAP+KGYFCR GR+YK+ARVCSYLG
Sbjct: 546 CALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLG 581

BLAST of Moc05g00180.1 vs. NCBI nr
Match: XP_022943972.1 (probable L-gulonolactone oxidase 6 [Cucurbita moschata])

HSP 1 Score: 954.9 bits (2467), Expect = 3.9e-274
Identity = 444/579 (76.68%), Postives = 514/579 (88.77%), Query Frame = 0

Query: 11  SFRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVY 70
           +FR  T  FFL  L+  SE +PPEDPIKCRSP  N++CTI N+Y TFPDR+ CR   V Y
Sbjct: 6   AFRFTTFIFFLVLLVSVSESTPPEDPIKCRSP--NANCTITNAYATFPDRAICRVGEVKY 65

Query: 71  PTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEA 130
           PTT+AE ISAVA ATRA+RK+KV TRFYHSIPKLACP G DG++ISTKYLNR LKVD E 
Sbjct: 66  PTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPHGVDGVVISTKYLNRVLKVDGEG 125

Query: 131 MTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYV 190
           M ITVEGGVT+R++IE AA  GLALPH PYWWGLTIGG++STGAHGSSLWGKGSAVHDYV
Sbjct: 126 MRITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYV 185

Query: 191 VEIQMVTPGGPQDGYAKLRRLLDGDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLK 250
           V I+MV+PGG QDGYAK+RRL+DG QHLNAAKVSLGVLGIITQVTLKL+PMFKRSITY +
Sbjct: 186 VGIKMVSPGGAQDGYAKVRRLVDGHQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFE 245

Query: 251 RHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSV 310
           R D DL D+V+SFGR+YEF D+ WYPSQR V+YR+D+RVSSNTSGNGLYDFIPFRPTPS 
Sbjct: 246 RDDTDLSDQVISFGRSYEFADMTWYPSQRKVMYRRDTRVSSNTSGNGLYDFIPFRPTPSA 305

Query: 311 GLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQS 370
            L ++RASEE QE+RRDA GKC SA+++TSLLS SAYGLTNNGITFLKYPVIGY NR+QS
Sbjct: 306 ALAIVRASEEYQEARRDAAGKCRSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQS 365

Query: 371 SGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCG 430
           SGSCL  +  D+RIT CPW+P IKGEFFH+  +S++L+VVK FIEDV+KL ELEPMAFCG
Sbjct: 366 SGSCL-DSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCG 425

Query: 431 LELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYG 490
           LELYNGILMRYVKAS+AYLGK+ DG++FDFTYYRS+DPMSPRLFEDVLEEVEQMA+FKYG
Sbjct: 426 LELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYG 485

Query: 491 GTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDG 550
           G+PHWGKNRN+AF EAI KY+++E FL++KNEYDP+GLFSN+WT+Q+LG++G V+IDKDG
Sbjct: 486 GSPHWGKNRNIAFLEAIGKYKNRERFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDG 545

Query: 551 CALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYLG 590
           CALEGLC+CSKDSHCAP+KGYFCR GR+YK+ARVCSYLG
Sbjct: 546 CALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLG 581

BLAST of Moc05g00180.1 vs. NCBI nr
Match: XP_038882597.1 (LOW QUALITY PROTEIN: probable L-gulonolactone oxidase 6 [Benincasa hispida])

HSP 1 Score: 950.3 bits (2455), Expect = 9.5e-273
Identity = 452/580 (77.93%), Postives = 509/580 (87.76%), Query Frame = 0

Query: 17  AFFFLFFLILGSECSPPEDPIKCR--SPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQ 76
           A  FL  +IL S+CSPPEDPIKC   SP+ +++CTI N+Y  FPDR+ CRA  V+YPTT+
Sbjct: 12  ALVFLLVVIL-SDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPDRTICRAAEVIYPTTE 71

Query: 77  AEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTIT 136
           AE + AVAAATR +RKMKV TRF HSIPKL+CP+G+DG++ISTKYLNRTL VD E M+I+
Sbjct: 72  AEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKYLNRTLMVDVEGMSIS 131

Query: 137 VEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQ 196
           VEGG T+RQ+IEE A AGLALPHTPYWWGLTIGG++STGAHGSSLWGKGSAVHDYVV IQ
Sbjct: 132 VEGGATVRQIIEEVAAAGLALPHTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAIQ 191

Query: 197 MVTPGGPQDGYAKLRRLLDGDQ-HLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHD 256
           MVTPGGPQDGYAKLR LL  DQ HLNAAKVSLGVLG+ITQVTLKLEPMFKRSITYLKR D
Sbjct: 192 MVTPGGPQDGYAKLRTLLPADQYHLNAAKVSLGVLGVITQVTLKLEPMFKRSITYLKRDD 251

Query: 257 RDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQ 316
           +DLGDEVVSFGR+YEFGD+IWYPSQR VVYR DSRV SNTSGNG YDFIPFRPTPS+ L 
Sbjct: 252 KDLGDEVVSFGRSYEFGDMIWYPSQRKVVYRIDSRVPSNTSGNGFYDFIPFRPTPSLALA 311

Query: 317 LLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGS 376
           L+RASEE QESRRDA GKC SA L TS LS  AYGLTNNGI+FLKYPVIG  NR+QSSGS
Sbjct: 312 LVRASEEKQESRRDAVGKCISANLFTSTLSQLAYGLTNNGISFLKYPVIGDSNRLQSSGS 371

Query: 377 CLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLEL 436
           CL+ +  D RIT CPWDPLIKGEFFHQTA+S++L VVKSF++DVQKLAELEPMAFCGLEL
Sbjct: 372 CLH-SLDDFRITACPWDPLIKGEFFHQTAISIDLGVVKSFVQDVQKLAELEPMAFCGLEL 431

Query: 437 YNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYGGTP 496
           YNGILMRYV AS+AYLGKQ  GVDFDFTYYRSRDPMSPRLFEDV EEVEQMAVFKYGG P
Sbjct: 432 YNGILMRYVTASTAYLGKQQHGVDFDFTYYRSRDPMSPRLFEDVFEEVEQMAVFKYGGIP 491

Query: 497 HWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCAL 556
           HWGKNRNVAF +A  KYR++++F+++  E+DPHGLFSN+WTDQ+LGLKG V I KDGCAL
Sbjct: 492 HWGKNRNVAFHQAFRKYRNRDSFVKIMKEFDPHGLFSNEWTDQILGLKGTVNIYKDGCAL 551

Query: 557 EGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYLGGSHK 594
           EGLCICS+ +HCAP +GYFC PGR+YK+ARVCSYLG S++
Sbjct: 552 EGLCICSQHTHCAPTEGYFCTPGRIYKEARVCSYLGSSYR 589

BLAST of Moc05g00180.1 vs. NCBI nr
Match: XP_008437774.1 (PREDICTED: probable L-gulonolactone oxidase 6 [Cucumis melo])

HSP 1 Score: 948.7 bits (2451), Expect = 2.8e-272
Identity = 444/569 (78.03%), Postives = 504/569 (88.58%), Query Frame = 0

Query: 23  FLILGSECSPPEDPIKCR--SPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQAEAISA 82
           FL+L SECSPPEDPIKC   SP+  ++CTI NSYG FPDRS CR   V+YPTT+AE + A
Sbjct: 23  FLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFPDRSICRVAEVIYPTTEAEVMLA 82

Query: 83  VAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTITVEGGVT 142
           VAAATR LRKMKV TRF HSIPKLACP+G++G++ISTKYLNRT+KVD E M+I+VEGG T
Sbjct: 83  VAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGAT 142

Query: 143 LRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQMVTPGG 202
           LRQ+IE+AATAGLALPHTPYWWGLTIGG++STGAHGSSLWGKGS+VHDYVV ++MV+PGG
Sbjct: 143 LRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAHGSSLWGKGSSVHDYVVAVKMVSPGG 202

Query: 203 PQDGYAKLRRLLDGDQ-HLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHDRDLGDE 262
            +DGYAKLR +L  DQ HLNAAKVSLGVLG+ITQVTLKL+PMFKRSITYLKR D+DLGDE
Sbjct: 203 SEDGYAKLRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGDE 262

Query: 263 VVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQLLRASE 322
           V+ FG +YEFGD+ WYPS R +VYR D+RV SNTSGNG+YDFIPFRPTPS+GL L+RASE
Sbjct: 263 VLRFGTHYEFGDMTWYPSHRKIVYRIDTRVPSNTSGNGVYDFIPFRPTPSIGLALIRASE 322

Query: 323 EDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGSCLYRNA 382
           E+QESRRDA  KC S  L+TS LS  AYGLTNNGITFLKYPVIGY NR+QSSGSCL  + 
Sbjct: 323 EEQESRRDAAAKCSSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCL-DSL 382

Query: 383 KDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLELYNGILM 442
            D+RIT CPWDP IKGEFFHQTA+S+EL +VKSFIEDVQ+LAELEPMAFCGLELYNGILM
Sbjct: 383 NDLRITTCPWDPSIKGEFFHQTAISIELTMVKSFIEDVQRLAELEPMAFCGLELYNGILM 442

Query: 443 RYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYGGTPHWGKNR 502
           RYV+AS+AYLGKQ DGVDFDFTYYRSRDPMSPRLFEDV+EEVEQMAVFKYGG PHWGKNR
Sbjct: 443 RYVRASTAYLGKQQDGVDFDFTYYRSRDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNR 502

Query: 503 NVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCALEGLCIC 562
           NVAF +   KYRD+E FL++  EYDPHGLFSN+WTDQ+LG++G V I KDGCALEGLCIC
Sbjct: 503 NVAFHQVFHKYRDREKFLKIMKEYDPHGLFSNEWTDQILGVEGTVIIYKDGCALEGLCIC 562

Query: 563 SKDSHCAPNKGYFCRPGRVYKDARVCSYL 589
           S+ +HCAP+KGYFCR GR+YKDARVCSYL
Sbjct: 563 SQHNHCAPSKGYFCRAGRIYKDARVCSYL 590

BLAST of Moc05g00180.1 vs. NCBI nr
Match: XP_022975260.1 (LOW QUALITY PROTEIN: probable L-gulonolactone oxidase 6 [Cucurbita maxima])

HSP 1 Score: 946.8 bits (2446), Expect = 1.1e-271
Identity = 441/579 (76.17%), Postives = 512/579 (88.43%), Query Frame = 0

Query: 11  SFRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVY 70
           +FR  T   FL  L+  SE +PPEDPIKCRSP  N++CTI N+Y TFPDR+ CR   V Y
Sbjct: 6   AFRFTTLILFLVLLVSVSESTPPEDPIKCRSP--NANCTITNAYATFPDRAICRVGEVKY 65

Query: 71  PTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEA 130
           PTT+AE ISAVAAATRA+RK+KV TRFYHSIPKLACPDG DG++ISTK+LN  LKVD E 
Sbjct: 66  PTTEAEMISAVAAATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKFLNWVLKVDGEG 125

Query: 131 MTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYV 190
           M+ITVEGGVT+R++IE AA  GLALPH PYW GLTIGG++STGAHGSSLWGKGSAVHDYV
Sbjct: 126 MSITVEGGVTVREIIEVAAAVGLALPHAPYWXGLTIGGLLSTGAHGSSLWGKGSAVHDYV 185

Query: 191 VEIQMVTPGGPQDGYAKLRRLLDGDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLK 250
           V ++MV+PGG QDGYAK+RRL+DGDQHLNAAKVSLGVLGIITQ TLKL+PMFKRSITY +
Sbjct: 186 VGMKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQFTLKLQPMFKRSITYFE 245

Query: 251 RHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSV 310
           R D DL DEV+SFGR+YEF D+ WYPSQR VVYR D+RVSSNTSGNGLYDFIPF PTPS 
Sbjct: 246 RDDTDLSDEVISFGRSYEFADMTWYPSQRKVVYRTDTRVSSNTSGNGLYDFIPFCPTPSA 305

Query: 311 GLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQS 370
           GL ++RAS+E QE+RRDA GKC SA+++TSLLS SAYGLTN+GITFLKYPVIGY NR+QS
Sbjct: 306 GLAIVRASKEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNDGITFLKYPVIGYNNRLQS 365

Query: 371 SGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCG 430
           SGSCL  +  D+RIT CPW+P IKGEFFH+  +S++L+VVK FIEDV+KL ELEPMAFCG
Sbjct: 366 SGSCL-DSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCG 425

Query: 431 LELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYG 490
           LELYNGILMRYVKAS+AYLGK+ DG++FDFTYYRS+DPMSPRLFEDVLEEVEQMA+FKYG
Sbjct: 426 LELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYG 485

Query: 491 GTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDG 550
           G PHWGKNRN+AF EAI KYR++E FL++KNEYDP+GLFSN+WT+Q+LG++G V+IDKDG
Sbjct: 486 GIPHWGKNRNIAFLEAIGKYRNREGFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDG 545

Query: 551 CALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYLG 590
           CALEGLC+CSKDSHCAP+KGYFCR GR+YK+ARVCSY+G
Sbjct: 546 CALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYIG 581

BLAST of Moc05g00180.1 vs. ExPASy Swiss-Prot
Match: O81032 (Probable L-gulonolactone oxidase 6 OS=Arabidopsis thaliana OX=3702 GN=GULLO6 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 3.6e-222
Identity = 365/565 (64.60%), Postives = 444/565 (78.58%), Query Frame = 0

Query: 24  LILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQAEAISAVAA 83
           L+  +  +PPEDP+KC S   N++CT+ NSYG FPDRSTCRA +V YPTT+AE IS VAA
Sbjct: 29  LVFTTISTPPEDPVKCVS--GNTNCTVTNSYGAFPDRSTCRAANVAYPTTEAELISVVAA 88

Query: 84  ATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTITVEGGVTLRQ 143
           AT+A RKM+V TR+ HSI KLAC DG DGLLISTK+LN T++ DA AMT+TVE GVTLRQ
Sbjct: 89  ATKAGRKMRVTTRYSHSITKLACTDGTDGLLISTKFLNHTVRTDATAMTLTVESGVTLRQ 148

Query: 144 LIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQMVTPGGPQD 203
           LI EAA  GLALP+ PYWWGLT+GGM+ TGAHGSSLWGKGSAVHDYV EI++V+PG   D
Sbjct: 149 LIAEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRIVSPGSVND 208

Query: 204 GYAKLRRLLD--GDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHDRDLGDEVV 263
           G+AK+R L +    +  NAAKVSLGVLG+I+QVTLKL+PMFKRS+ Y+ R+D D GD+ V
Sbjct: 209 GFAKVRVLRETTTPKEFNAAKVSLGVLGVISQVTLKLQPMFKRSLRYVMRNDSDFGDQAV 268

Query: 264 SFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQLLRASEED 323
           +FG  +EF D IW PSQ  VVYR D RV+ NTSGNGL DF+PFR   S  L ++R+SEE 
Sbjct: 269 TFGMKHEFADFIWLPSQGKVVYRMDDRVAVNTSGNGLLDFMPFRSQLSAALAIIRSSEET 328

Query: 324 QESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGSCLYRNAKD 383
           QE  RDA GKC  A L+TS L  ++YGLTNNG+ F  YPVIG QNR+ SSGSCL  +  D
Sbjct: 329 QERFRDANGKCAGATLITSTLFATSYGLTNNGMIFTGYPVIGSQNRMMSSGSCL-DSLHD 388

Query: 384 MRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLELYNGILMRY 443
             IT CPWD  IK EFFHQT  S+ L  VKSFI D++ L ++E  + C LELY+GILMRY
Sbjct: 389 GLITACPWDSRIKSEFFHQTTFSIPLTQVKSFINDIKSLVKIESKSLCVLELYDGILMRY 448

Query: 444 VKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYGGTPHWGKNRNV 503
           V +S AYLGK+ + +DFD TYYR++DP+SPRL+ED +EE+EQ+A+FKY   PHWGKNRN+
Sbjct: 449 VTSSPAYLGKETEALDFDLTYYRAKDPLSPRLYEDFIEEIEQIALFKYNALPHWGKNRNL 508

Query: 504 AFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCALEGLCICSK 563
           AF   I KY++  AFL+VK  YDP GLFS++WTDQ+LG+KG VTI KDGCALEGLC+CS+
Sbjct: 509 AFDGVIKKYKNVPAFLKVKESYDPMGLFSSEWTDQILGIKGNVTIIKDGCALEGLCVCSE 568

Query: 564 DSHCAPNKGYFCRPGRVYKDARVCS 587
           D+HCAP KGYFCRPG+VYK+ARVC+
Sbjct: 569 DAHCAPTKGYFCRPGKVYKEARVCT 590

BLAST of Moc05g00180.1 vs. ExPASy Swiss-Prot
Match: Q6NQ66 (L-gulonolactone oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GULLO2 PE=1 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.4e-218
Identity = 356/582 (61.17%), Postives = 448/582 (76.98%), Query Frame = 0

Query: 11  SFRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVY 70
           S+R     +++F L+  +  +PP+DP+KC S   N++CT+ NSYG FPDRSTCRA +V Y
Sbjct: 8   SYRTLVGLYYIFTLMHTAVSTPPDDPVKCVS--GNTNCTVTNSYGAFPDRSTCRAANVAY 67

Query: 71  PTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEA 130
           P T+AE +S VAAAT+A RKM+V TR+ HSI KL C DG +GL ISTK+LN T++ DA A
Sbjct: 68  PKTEAELVSVVAAATQAGRKMRVTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQADATA 127

Query: 131 MTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYV 190
           MT+TVE G+TLRQLI EAA  GLALP+ PYWWGLT+GGM+ TGAHGSSLWGKGSAVHDYV
Sbjct: 128 MTMTVESGMTLRQLIVEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYV 187

Query: 191 VEIQMVTPGGPQDGYAKLRRLLD--GDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITY 250
            EI+MV+PG   +G+AK+R L +       NAAKVSLGVLG+I+QVT +L+PMFKRS+TY
Sbjct: 188 TEIRMVSPGSVNEGFAKIRILSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLTY 247

Query: 251 LKRHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTP 310
             R+D D  D+ V+FG+ +EF D IW PSQ  VVYR+D RV+ NTSGNGL+DF+PFR   
Sbjct: 248 TMRNDSDFEDQAVTFGKKHEFADFIWLPSQGKVVYRRDDRVAVNTSGNGLFDFLPFRSQL 307

Query: 311 SVGLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRV 370
           S  + ++R SEE QE  RDA GKC  A +++S L   +YGLTNNGI F  YPV+G QNR+
Sbjct: 308 SAAIAIIRTSEETQERFRDANGKCVGATIISSTLFAPSYGLTNNGIIFTGYPVVGSQNRM 367

Query: 371 QSSGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAF 430
            SSGSCL  + +D  IT C WD  IKGEFFHQT +SV L  VKSFI D++ L ++E  + 
Sbjct: 368 MSSGSCL-DSLQDGLITACAWDSRIKGEFFHQTTLSVPLTQVKSFISDIKSLVKIEQKSL 427

Query: 431 CGLELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFK 490
           CGLEL+ GILMRYV +S AYLGK+ + +DFD TYYR++DP++PRL+ED +EE+EQ+A+FK
Sbjct: 428 CGLELHYGILMRYVTSSPAYLGKETEALDFDITYYRAKDPLTPRLYEDFIEEIEQIALFK 487

Query: 491 YGGTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDK 550
           Y   PHWGKNRN+AF   I KY +  AFL+VK+ YDP GLFS++WTDQ+LG+KG  +I K
Sbjct: 488 YNALPHWGKNRNLAFDGVIRKYNNAPAFLKVKDSYDPKGLFSSEWTDQILGIKGNASIVK 547

Query: 551 DGCALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYLGG 591
           DGCALEGLCICSKD+HCAP KGY CRPG+VYK+ARVC+ + G
Sbjct: 548 DGCALEGLCICSKDAHCAPAKGYLCRPGKVYKEARVCTRVDG 586

BLAST of Moc05g00180.1 vs. ExPASy Swiss-Prot
Match: O81030 (L-gulonolactone oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=GULLO5 PE=1 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 2.5e-215
Identity = 352/579 (60.79%), Postives = 440/579 (75.99%), Query Frame = 0

Query: 12  FRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVYP 71
           +R     + LF L+     +PPEDP+KC S   N++C + NS G FPDRSTCRA +V YP
Sbjct: 13  WRTLLGLYCLFTLVHTVISTPPEDPVKCVS--GNTNCIVTNSLGAFPDRSTCRAANVAYP 72

Query: 72  TTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAM 131
           TT+AE +S VAAAT+A RKM+V TR+ HSIPKL C DG DGL ISTK+LN T++ DA+AM
Sbjct: 73  TTEAELVSIVAAATKAGRKMRVTTRYSHSIPKLTCTDGNDGLFISTKFLNHTVQADAKAM 132

Query: 132 TITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVV 191
           T+TVE GVTLRQLI EAA  GLALP+ PYWWG+T+GGM+ TGAHGSSLWGKGSAVHDYV 
Sbjct: 133 TLTVESGVTLRQLIAEAAKVGLALPYAPYWWGVTVGGMMGTGAHGSSLWGKGSAVHDYVT 192

Query: 192 EIQMVTPGGPQDGYAKLRRLLD--GDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYL 251
           EI+MV+PG   DG+AK+R L +       NAAKVSLGVLG+I+QVT +L+PMFKRS+ Y+
Sbjct: 193 EIRMVSPGSVNDGFAKIRVLSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLKYV 252

Query: 252 KRHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPS 311
            R+D D  DE ++FG+ +EF D +W PSQ  VVYR D RV+ NT GNGLYDF PFR   S
Sbjct: 253 MRNDLDFNDEALTFGKKHEFADFVWLPSQGKVVYRMDDRVAVNTLGNGLYDFFPFRSQLS 312

Query: 312 VGLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQ 371
             L   R+SEE QE+ RDA GKC +A  ++S L  ++YGLTNNGITF  YPVIG QNR+ 
Sbjct: 313 AVLATTRSSEETQETLRDAHGKCVTATTISSTLFSTSYGLTNNGITFTGYPVIGSQNRMM 372

Query: 372 SSGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFC 431
           SSGSCL    +D   + C WD  +KG F+HQT  S+ L  VKSFI D++ L +++  + C
Sbjct: 373 SSGSCL-DGLEDKLTSACAWDSRVKGVFYHQTTFSIPLTQVKSFINDIKSLLKIDSKSLC 432

Query: 432 GLELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKY 491
           GLELY GILMRYV +S AYLGK+ + +DFD TYYR+ DP++PRL+ED +EE+EQ+A+ KY
Sbjct: 433 GLELYYGILMRYVTSSPAYLGKETEAIDFDITYYRANDPLTPRLYEDFIEEIEQIALLKY 492

Query: 492 GGTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKD 551
              PHWGKNRN+AF   I KY++  AFL+VK  YDP+GLFS++WTDQ+LG+KG  TI KD
Sbjct: 493 NALPHWGKNRNLAFDGVIKKYKNAPAFLKVKESYDPNGLFSSEWTDQILGIKGNPTIVKD 552

Query: 552 GCALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYL 589
           GCALEGLCICS D+HCAP+KGY CRPG+VYK+ARVC+++
Sbjct: 553 GCALEGLCICSDDAHCAPSKGYLCRPGKVYKEARVCTHV 588

BLAST of Moc05g00180.1 vs. ExPASy Swiss-Prot
Match: Q9C614 (Probable L-gulonolactone oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GULLO1 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 8.5e-208
Identity = 345/574 (60.10%), Postives = 430/574 (74.91%), Query Frame = 0

Query: 19  FFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQAEAI 78
           FF F     S  +PP+DP+KC S   N+ CT+ NSYG FPDRS C A  V YP T+AE +
Sbjct: 9   FFCFCTFASS--TPPDDPVKCES--GNNMCTVTNSYGAFPDRSICEAAKVEYPKTEAELV 68

Query: 79  SAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTITVEGG 138
           S VAAATRA +K++V TR+ HSIPKL C DG+DG+LISTK+LN  +  + EA T+TVE G
Sbjct: 69  SIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEAKTLTVESG 128

Query: 139 VTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQMVTP 198
           VTLRQLI EAA   LALPH PYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV EI+MV+P
Sbjct: 129 VTLRQLIGEAAELELALPHAPYWWGLTVGGLMGTGAHGSSLWGKGSAVHDYVSEIRMVSP 188

Query: 199 GGPQDGYAKLRRL---LDGDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHDRD 258
           G   DGY K+R L   +D D+   AAKVSLGVLG+I+QVT +L+PMFKRS+T++ ++D D
Sbjct: 189 GLASDGYVKVRVLSETIDPDE-FRAAKVSLGVLGVISQVTFQLQPMFKRSLTFVMQNDSD 248

Query: 259 LGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQLL 318
            GD+ V+FG  +EF D +W PSQ  VVYR D RV  NTSGNGL+DF PFRP  SV L ++
Sbjct: 249 FGDQAVTFGEKHEFADFLWLPSQGKVVYRMDDRVPVNTSGNGLFDFFPFRPQLSVALAII 308

Query: 319 RASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGSCL 378
           R+ EE +ES  DA  KC  A  +TS L   +YG+TNNG+ F  YPVIG QN + SSG+CL
Sbjct: 309 RSLEESEESSGDANDKCARAEQITSFLFSISYGVTNNGMEFTGYPVIGKQNHMMSSGTCL 368

Query: 379 YRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLELYN 438
             + +D  IT CPWDP IKG+FFHQTA S+ L  VK FI D++ L ++EP + C LE  N
Sbjct: 369 -DSHQDGLITSCPWDPRIKGQFFHQTAFSIPLTRVKGFINDIKALVKIEPKSLCALERSN 428

Query: 439 GILMRYVKASSAYLGKQHDGVDFDFTYYRSR-DPMSPRLFEDVLEEVEQMAVFKYGGTPH 498
           GIL+RYV +S A+LGK+   +DFD TYYRS+ DP++PRL+ED +EE+EQMA+FKY   PH
Sbjct: 429 GILIRYVTSSPAFLGKEEKALDFDLTYYRSKDDPLAPRLYEDFIEEIEQMAIFKYNALPH 488

Query: 499 WGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCALE 558
           WGKNRN+AF   I KY++   FL+VK  +DP GLFS +WT+Q+LGLKG VTI K+GCALE
Sbjct: 489 WGKNRNLAFDGVIRKYKNANTFLKVKERFDPLGLFSTEWTNQILGLKGNVTIVKEGCALE 548

Query: 559 GLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYL 589
           GLC+CS D+HCAP KGY CRPG+VY  ARVC+++
Sbjct: 549 GLCVCSDDAHCAPKKGYLCRPGKVYTKARVCTHV 576

BLAST of Moc05g00180.1 vs. ExPASy Swiss-Prot
Match: Q9FM82 (Probable L-gulonolactone oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GULLO4 PE=2 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 1.2e-206
Identity = 345/573 (60.21%), Postives = 428/573 (74.69%), Query Frame = 0

Query: 17  AFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQAE 76
           +  F FF    S  SPP+DP+ C     N+ CT+ NSYG FPDRS C+A  V YP T+AE
Sbjct: 6   SLIFCFFTFASS--SPPDDPVNCE--FGNTMCTVTNSYGAFPDRSICKAAKVEYPRTEAE 65

Query: 77  AISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTITVE 136
            +S VAAATRA +KM+V TR+ HS PKL C DG+DG LISTK+LN  +  + EA T+TVE
Sbjct: 66  LVSIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEAKTLTVE 125

Query: 137 GGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQMV 196
            GVTLRQLIEEAA   LALP+ PYWWGLT+GGM+ TGAHGSSLWGKGSAVHDYV EI++V
Sbjct: 126 SGVTLRQLIEEAAKFDLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRLV 185

Query: 197 TPGGPQDGYAKLRRLLD--GDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHDR 256
           +PG   DGY K++ L +    +   AAKVSLGVLG+I+QVT +L+PMFKRS+ Y+ R+D 
Sbjct: 186 SPGLASDGYVKVQVLSETIDPEEFRAAKVSLGVLGVISQVTFELQPMFKRSLNYVMRNDS 245

Query: 257 DLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQL 316
           D GD+ VSFG  +EF D +W PSQ  VVYR D RV  NTSG+GL++F PFR   S+ L +
Sbjct: 246 DFGDQAVSFGERHEFADFLWLPSQGKVVYRMDGRVPLNTSGDGLFEFFPFRSQLSLVLAI 305

Query: 317 LRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGSC 376
            R+ EE +ES  DA  KC  A+LV+S +   +YG+TNNG+ F  YPVIG QN + SSGSC
Sbjct: 306 DRSLEESEESLEDANMKCVRAKLVSSSMFLMSYGVTNNGLIFTGYPVIGMQNHMMSSGSC 365

Query: 377 LYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLELY 436
           L  + +D  IT CPWDP IKG+FFHQT  SV L  VKSFI D++ L ++EP + C LE  
Sbjct: 366 L-DSRQDGLITACPWDPRIKGQFFHQTTFSVSLTNVKSFINDIKALVKIEPKSLCVLEGS 425

Query: 437 NGILMRYVKASSAYLGKQHDGVDFDFTYYRSR-DPMSPRLFEDVLEEVEQMAVFKYGGTP 496
           NGIL+RYV +S A+LGK+   +DFD TYYRS+ DP++PRL+ED +EE+EQMA+FKY   P
Sbjct: 426 NGILIRYVTSSLAFLGKEEKALDFDLTYYRSKNDPLAPRLYEDYIEEIEQMAIFKYNALP 485

Query: 497 HWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCAL 556
           HWGKNRN+AF  AI KY++  AFL+VK ++D  GLFS +WTDQ+LGLKG VTI K GCAL
Sbjct: 486 HWGKNRNLAFDGAIRKYKNANAFLKVKEKFDSLGLFSTEWTDQILGLKGNVTIVKQGCAL 545

Query: 557 EGLCICSKDSHCAPNKGYFCRPGRVYKDARVCS 587
           EGLCICS+DSHCAP KGY CRPG+VY++ARVC+
Sbjct: 546 EGLCICSEDSHCAPTKGYLCRPGKVYREARVCT 573

BLAST of Moc05g00180.1 vs. ExPASy TrEMBL
Match: A0A6J1FUH8 (L-gulonolactone oxidase OS=Cucurbita moschata OX=3662 GN=LOC111448536 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.9e-274
Identity = 444/579 (76.68%), Postives = 514/579 (88.77%), Query Frame = 0

Query: 11  SFRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVY 70
           +FR  T  FFL  L+  SE +PPEDPIKCRSP  N++CTI N+Y TFPDR+ CR   V Y
Sbjct: 6   AFRFTTFIFFLVLLVSVSESTPPEDPIKCRSP--NANCTITNAYATFPDRAICRVGEVKY 65

Query: 71  PTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEA 130
           PTT+AE ISAVA ATRA+RK+KV TRFYHSIPKLACP G DG++ISTKYLNR LKVD E 
Sbjct: 66  PTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPHGVDGVVISTKYLNRVLKVDGEG 125

Query: 131 MTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYV 190
           M ITVEGGVT+R++IE AA  GLALPH PYWWGLTIGG++STGAHGSSLWGKGSAVHDYV
Sbjct: 126 MRITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYV 185

Query: 191 VEIQMVTPGGPQDGYAKLRRLLDGDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLK 250
           V I+MV+PGG QDGYAK+RRL+DG QHLNAAKVSLGVLGIITQVTLKL+PMFKRSITY +
Sbjct: 186 VGIKMVSPGGAQDGYAKVRRLVDGHQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFE 245

Query: 251 RHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSV 310
           R D DL D+V+SFGR+YEF D+ WYPSQR V+YR+D+RVSSNTSGNGLYDFIPFRPTPS 
Sbjct: 246 RDDTDLSDQVISFGRSYEFADMTWYPSQRKVMYRRDTRVSSNTSGNGLYDFIPFRPTPSA 305

Query: 311 GLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQS 370
            L ++RASEE QE+RRDA GKC SA+++TSLLS SAYGLTNNGITFLKYPVIGY NR+QS
Sbjct: 306 ALAIVRASEEYQEARRDAAGKCRSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQS 365

Query: 371 SGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCG 430
           SGSCL  +  D+RIT CPW+P IKGEFFH+  +S++L+VVK FIEDV+KL ELEPMAFCG
Sbjct: 366 SGSCL-DSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCG 425

Query: 431 LELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYG 490
           LELYNGILMRYVKAS+AYLGK+ DG++FDFTYYRS+DPMSPRLFEDVLEEVEQMA+FKYG
Sbjct: 426 LELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYG 485

Query: 491 GTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDG 550
           G+PHWGKNRN+AF EAI KY+++E FL++KNEYDP+GLFSN+WT+Q+LG++G V+IDKDG
Sbjct: 486 GSPHWGKNRNIAFLEAIGKYKNRERFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDG 545

Query: 551 CALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYLG 590
           CALEGLC+CSKDSHCAP+KGYFCR GR+YK+ARVCSYLG
Sbjct: 546 CALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLG 581

BLAST of Moc05g00180.1 vs. ExPASy TrEMBL
Match: A0A1S3AUG3 (L-gulonolactone oxidase OS=Cucumis melo OX=3656 GN=LOC103483108 PE=3 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 1.3e-272
Identity = 444/569 (78.03%), Postives = 504/569 (88.58%), Query Frame = 0

Query: 23  FLILGSECSPPEDPIKCR--SPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQAEAISA 82
           FL+L SECSPPEDPIKC   SP+  ++CTI NSYG FPDRS CR   V+YPTT+AE + A
Sbjct: 23  FLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFPDRSICRVAEVIYPTTEAEVMLA 82

Query: 83  VAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTITVEGGVT 142
           VAAATR LRKMKV TRF HSIPKLACP+G++G++ISTKYLNRT+KVD E M+I+VEGG T
Sbjct: 83  VAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGAT 142

Query: 143 LRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQMVTPGG 202
           LRQ+IE+AATAGLALPHTPYWWGLTIGG++STGAHGSSLWGKGS+VHDYVV ++MV+PGG
Sbjct: 143 LRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAHGSSLWGKGSSVHDYVVAVKMVSPGG 202

Query: 203 PQDGYAKLRRLLDGDQ-HLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHDRDLGDE 262
            +DGYAKLR +L  DQ HLNAAKVSLGVLG+ITQVTLKL+PMFKRSITYLKR D+DLGDE
Sbjct: 203 SEDGYAKLRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGDE 262

Query: 263 VVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQLLRASE 322
           V+ FG +YEFGD+ WYPS R +VYR D+RV SNTSGNG+YDFIPFRPTPS+GL L+RASE
Sbjct: 263 VLRFGTHYEFGDMTWYPSHRKIVYRIDTRVPSNTSGNGVYDFIPFRPTPSIGLALIRASE 322

Query: 323 EDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGSCLYRNA 382
           E+QESRRDA  KC S  L+TS LS  AYGLTNNGITFLKYPVIGY NR+QSSGSCL  + 
Sbjct: 323 EEQESRRDAAAKCSSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCL-DSL 382

Query: 383 KDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLELYNGILM 442
            D+RIT CPWDP IKGEFFHQTA+S+EL +VKSFIEDVQ+LAELEPMAFCGLELYNGILM
Sbjct: 383 NDLRITTCPWDPSIKGEFFHQTAISIELTMVKSFIEDVQRLAELEPMAFCGLELYNGILM 442

Query: 443 RYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYGGTPHWGKNR 502
           RYV+AS+AYLGKQ DGVDFDFTYYRSRDPMSPRLFEDV+EEVEQMAVFKYGG PHWGKNR
Sbjct: 443 RYVRASTAYLGKQQDGVDFDFTYYRSRDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNR 502

Query: 503 NVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCALEGLCIC 562
           NVAF +   KYRD+E FL++  EYDPHGLFSN+WTDQ+LG++G V I KDGCALEGLCIC
Sbjct: 503 NVAFHQVFHKYRDREKFLKIMKEYDPHGLFSNEWTDQILGVEGTVIIYKDGCALEGLCIC 562

Query: 563 SKDSHCAPNKGYFCRPGRVYKDARVCSYL 589
           S+ +HCAP+KGYFCR GR+YKDARVCSYL
Sbjct: 563 SQHNHCAPSKGYFCRAGRIYKDARVCSYL 590

BLAST of Moc05g00180.1 vs. ExPASy TrEMBL
Match: A0A6J1IG87 (L-gulonolactone oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474382 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 5.1e-272
Identity = 441/579 (76.17%), Postives = 512/579 (88.43%), Query Frame = 0

Query: 11  SFRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVY 70
           +FR  T   FL  L+  SE +PPEDPIKCRSP  N++CTI N+Y TFPDR+ CR   V Y
Sbjct: 6   AFRFTTLILFLVLLVSVSESTPPEDPIKCRSP--NANCTITNAYATFPDRAICRVGEVKY 65

Query: 71  PTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEA 130
           PTT+AE ISAVAAATRA+RK+KV TRFYHSIPKLACPDG DG++ISTK+LN  LKVD E 
Sbjct: 66  PTTEAEMISAVAAATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKFLNWVLKVDGEG 125

Query: 131 MTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYV 190
           M+ITVEGGVT+R++IE AA  GLALPH PYW GLTIGG++STGAHGSSLWGKGSAVHDYV
Sbjct: 126 MSITVEGGVTVREIIEVAAAVGLALPHAPYWXGLTIGGLLSTGAHGSSLWGKGSAVHDYV 185

Query: 191 VEIQMVTPGGPQDGYAKLRRLLDGDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLK 250
           V ++MV+PGG QDGYAK+RRL+DGDQHLNAAKVSLGVLGIITQ TLKL+PMFKRSITY +
Sbjct: 186 VGMKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQFTLKLQPMFKRSITYFE 245

Query: 251 RHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSV 310
           R D DL DEV+SFGR+YEF D+ WYPSQR VVYR D+RVSSNTSGNGLYDFIPF PTPS 
Sbjct: 246 RDDTDLSDEVISFGRSYEFADMTWYPSQRKVVYRTDTRVSSNTSGNGLYDFIPFCPTPSA 305

Query: 311 GLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQS 370
           GL ++RAS+E QE+RRDA GKC SA+++TSLLS SAYGLTN+GITFLKYPVIGY NR+QS
Sbjct: 306 GLAIVRASKEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNDGITFLKYPVIGYNNRLQS 365

Query: 371 SGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCG 430
           SGSCL  +  D+RIT CPW+P IKGEFFH+  +S++L+VVK FIEDV+KL ELEPMAFCG
Sbjct: 366 SGSCL-DSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCG 425

Query: 431 LELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYG 490
           LELYNGILMRYVKAS+AYLGK+ DG++FDFTYYRS+DPMSPRLFEDVLEEVEQMA+FKYG
Sbjct: 426 LELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYG 485

Query: 491 GTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDG 550
           G PHWGKNRN+AF EAI KYR++E FL++KNEYDP+GLFSN+WT+Q+LG++G V+IDKDG
Sbjct: 486 GIPHWGKNRNIAFLEAIGKYRNREGFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDG 545

Query: 551 CALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYLG 590
           CALEGLC+CSKDSHCAP+KGYFCR GR+YK+ARVCSY+G
Sbjct: 546 CALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYIG 581

BLAST of Moc05g00180.1 vs. ExPASy TrEMBL
Match: A0A0A0L3K4 (L-gulonolactone oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G116800 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 8.7e-264
Identity = 445/605 (73.55%), Postives = 502/605 (82.98%), Query Frame = 0

Query: 4   WRRSCDLSFRRFTAFFFLFFLILGSECSPPEDPIKC--RSPHDNSDCTIANSYGTFPDRS 63
           WR  C +S      F    FL+L SECSPP DPIKC   SP+  ++CTI N YG+FPDRS
Sbjct: 6   WRSYCVVSCPSSVVF---MFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRS 65

Query: 64  TCRARHVVYPTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLN 123
            CR   V+YPTT+AE + AVAAATR LRKMKV TRF HSIPKLACP+G++G++ISTKYLN
Sbjct: 66  ICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLN 125

Query: 124 RTLKVDAEAMTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWG 183
           RT+KVD E M+I+VEGG TLRQ+IE+AATAGLALP TPYWWGLTIGG++STGAHGSSLWG
Sbjct: 126 RTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWG 185

Query: 184 KGSAVHDYVVEIQMVTPGGPQDGYAKLRRLLDGDQ-HLNAAKVSLGVLGIITQVTLKLEP 243
           KGSAVHDYVV +QMV+PGGP+DGYAK R +L  DQ HLNAAKVSLGVLG+ITQVTLKL+P
Sbjct: 186 KGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQP 245

Query: 244 MFKRSITYLKRHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYD 303
           MFKRSITYLKR D+DLGDEV+ FG++YEFGD+ WYPSQR VVYR D+RV SNTSGNGLYD
Sbjct: 246 MFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMSWYPSQRKVVYRIDTRVPSNTSGNGLYD 305

Query: 304 FIPFRPTPSVGLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNN-------- 363
           FIPFRPT SV L LLRASEE+QESRRDA  KC S  L+TS LS  AYGLTNN        
Sbjct: 306 FIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNVILNKMYN 365

Query: 364 ---------GITFLKYPVIGYQNRVQSSGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAV 423
                    GITFLKYPVIGY NR+QSSGSCL  +  D RIT CPWDP IKGEFF++T+V
Sbjct: 366 IYMSYINIVGITFLKYPVIGYNNRLQSSGSCL-DSLNDFRITACPWDPSIKGEFFYETSV 425

Query: 424 SVELRVVKSFIEDVQKLAELEPMAFCGLELYNGILMRYVKASSAYLGKQHDGVDFDFTYY 483
           S+EL VVKSFIEDVQKLAELEPMAFCGLELYNGILMRYV AS+AYLGKQ D V+FD TYY
Sbjct: 426 SIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYY 485

Query: 484 RSRDPMSPRLFEDVLEEVEQMAVFKYGGTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEY 543
           RS+DPMSPRLFEDV+EEVEQMAVFKYGG PHWGKNRNVAF +   KYR+ E FL++  EY
Sbjct: 486 RSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEY 545

Query: 544 DPHGLFSNQWTDQVLGLKGAVTIDKDGCALEGLCICSKDSHCAPNKGYFCRPGRVYKDAR 589
           DPH LFSN+WTDQ+LG++G V I KDGCALEGLCICSK +HCAP+KGYFCR G++YKDAR
Sbjct: 546 DPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDAR 605

BLAST of Moc05g00180.1 vs. ExPASy TrEMBL
Match: A0A5C7I1X0 (L-gulonolactone oxidase OS=Acer yangbiense OX=1000413 GN=EZV62_010088 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 7.4e-239
Identity = 393/580 (67.76%), Postives = 468/580 (80.69%), Query Frame = 0

Query: 11  SFRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVY 70
           +FR       LF L+    C+PP+DPIKC S  + S CTI NSYG FPDRS C+A +V Y
Sbjct: 13  TFRHLFRSKCLFLLVFIVSCTPPDDPIKC-SSSNTSSCTITNSYGMFPDRSVCKAANVAY 72

Query: 71  PTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEA 130
           PTT+ E IS V+AAT+A+RKMKVATRF HSIPKL CPDG+DGLLISTKYLNR LK+DA+ 
Sbjct: 73  PTTEQELISVVSAATKAMRKMKVATRFSHSIPKLVCPDGEDGLLISTKYLNRVLKIDAQV 132

Query: 131 MTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYV 190
           MT+TVE GVTLRQ+IEEAA AGLALP+ PYWWGLTIGG++ TGAHGS+LW KGS+VHDYV
Sbjct: 133 MTMTVESGVTLRQIIEEAAKAGLALPYAPYWWGLTIGGLIGTGAHGSTLWSKGSSVHDYV 192

Query: 191 VEIQMVTPGGPQDGYAKLRRLLDGD---QHLNAAKVSLGVLGIITQVTLKLEPMFKRSIT 250
            E+++V+PGG ++ Y K+R L + D   Q L+AAKVSLGVLG+I+QVTLKL+P+FKRSI 
Sbjct: 193 SELKIVSPGGRENNYVKVRVLNENDQDRQDLDAAKVSLGVLGVISQVTLKLQPLFKRSIR 252

Query: 251 YLKRHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPT 310
           Y+  +D DLGD+  SFG  +EF D++WYPSQR  VYR D R+SSNTSGNGLYD+IPFR T
Sbjct: 253 YMTTNDSDLGDQAASFGHQHEFADIVWYPSQRKAVYRIDDRISSNTSGNGLYDYIPFRST 312

Query: 311 PSVGLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNR 370
           PS+ L L+R +EE+QE+R DA GKCF A+ +TS L  SA+GLTNNG+TF  YPVIGY NR
Sbjct: 313 PSLALSLVRTTEENQETRSDADGKCFVAKFLTSTLWTSAFGLTNNGVTFTGYPVIGYHNR 372

Query: 371 VQSSGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMA 430
           +QSSG+CL  +  D  IT CPWDP IKGEFFHQT  SV L VVKSFI+DVQKL  LEP A
Sbjct: 373 LQSSGTCL-DSLGDSMITSCPWDPRIKGEFFHQTTFSVGLSVVKSFIQDVQKLVSLEPKA 432

Query: 431 FCGLELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVF 490
            CG+ELYNGILMRYVKASSAYLGKQ D +DFD TYYRS+DPM+PRL++DVLEE+EQ+AVF
Sbjct: 433 LCGIELYNGILMRYVKASSAYLGKQEDSLDFDITYYRSKDPMTPRLYQDVLEEIEQLAVF 492

Query: 491 KYGGTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTID 550
           KYG  PHWGKNRNV F   I KY++   F +VK  YDP GLFS++WTDQVL LK  +TID
Sbjct: 493 KYGALPHWGKNRNVVFDGVIKKYKNAGKFFKVKVAYDPLGLFSSEWTDQVLSLKAGMTID 552

Query: 551 KDGCALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSY 588
           + GCALEGLCICS+DSHCAP KGY C+PG+VYK ARVC++
Sbjct: 553 RQGCALEGLCICSQDSHCAPEKGYLCKPGKVYKHARVCTF 590

BLAST of Moc05g00180.1 vs. TAIR 10
Match: AT2G46760.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 772.7 bits (1994), Expect = 2.5e-223
Identity = 365/565 (64.60%), Postives = 444/565 (78.58%), Query Frame = 0

Query: 24  LILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQAEAISAVAA 83
           L+  +  +PPEDP+KC S   N++CT+ NSYG FPDRSTCRA +V YPTT+AE IS VAA
Sbjct: 29  LVFTTISTPPEDPVKCVS--GNTNCTVTNSYGAFPDRSTCRAANVAYPTTEAELISVVAA 88

Query: 84  ATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTITVEGGVTLRQ 143
           AT+A RKM+V TR+ HSI KLAC DG DGLLISTK+LN T++ DA AMT+TVE GVTLRQ
Sbjct: 89  ATKAGRKMRVTTRYSHSITKLACTDGTDGLLISTKFLNHTVRTDATAMTLTVESGVTLRQ 148

Query: 144 LIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQMVTPGGPQD 203
           LI EAA  GLALP+ PYWWGLT+GGM+ TGAHGSSLWGKGSAVHDYV EI++V+PG   D
Sbjct: 149 LIAEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRIVSPGSVND 208

Query: 204 GYAKLRRLLD--GDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHDRDLGDEVV 263
           G+AK+R L +    +  NAAKVSLGVLG+I+QVTLKL+PMFKRS+ Y+ R+D D GD+ V
Sbjct: 209 GFAKVRVLRETTTPKEFNAAKVSLGVLGVISQVTLKLQPMFKRSLRYVMRNDSDFGDQAV 268

Query: 264 SFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQLLRASEED 323
           +FG  +EF D IW PSQ  VVYR D RV+ NTSGNGL DF+PFR   S  L ++R+SEE 
Sbjct: 269 TFGMKHEFADFIWLPSQGKVVYRMDDRVAVNTSGNGLLDFMPFRSQLSAALAIIRSSEET 328

Query: 324 QESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGSCLYRNAKD 383
           QE  RDA GKC  A L+TS L  ++YGLTNNG+ F  YPVIG QNR+ SSGSCL  +  D
Sbjct: 329 QERFRDANGKCAGATLITSTLFATSYGLTNNGMIFTGYPVIGSQNRMMSSGSCL-DSLHD 388

Query: 384 MRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLELYNGILMRY 443
             IT CPWD  IK EFFHQT  S+ L  VKSFI D++ L ++E  + C LELY+GILMRY
Sbjct: 389 GLITACPWDSRIKSEFFHQTTFSIPLTQVKSFINDIKSLVKIESKSLCVLELYDGILMRY 448

Query: 444 VKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKYGGTPHWGKNRNV 503
           V +S AYLGK+ + +DFD TYYR++DP+SPRL+ED +EE+EQ+A+FKY   PHWGKNRN+
Sbjct: 449 VTSSPAYLGKETEALDFDLTYYRAKDPLSPRLYEDFIEEIEQIALFKYNALPHWGKNRNL 508

Query: 504 AFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCALEGLCICSK 563
           AF   I KY++  AFL+VK  YDP GLFS++WTDQ+LG+KG VTI KDGCALEGLC+CS+
Sbjct: 509 AFDGVIKKYKNVPAFLKVKESYDPMGLFSSEWTDQILGIKGNVTIIKDGCALEGLCVCSE 568

Query: 564 DSHCAPNKGYFCRPGRVYKDARVCS 587
           D+HCAP KGYFCRPG+VYK+ARVC+
Sbjct: 569 DAHCAPTKGYFCRPGKVYKEARVCT 590

BLAST of Moc05g00180.1 vs. TAIR 10
Match: AT2G46750.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 760.8 bits (1963), Expect = 9.9e-220
Identity = 356/582 (61.17%), Postives = 448/582 (76.98%), Query Frame = 0

Query: 11  SFRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVY 70
           S+R     +++F L+  +  +PP+DP+KC S   N++CT+ NSYG FPDRSTCRA +V Y
Sbjct: 8   SYRTLVGLYYIFTLMHTAVSTPPDDPVKCVS--GNTNCTVTNSYGAFPDRSTCRAANVAY 67

Query: 71  PTTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEA 130
           P T+AE +S VAAAT+A RKM+V TR+ HSI KL C DG +GL ISTK+LN T++ DA A
Sbjct: 68  PKTEAELVSVVAAATQAGRKMRVTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQADATA 127

Query: 131 MTITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYV 190
           MT+TVE G+TLRQLI EAA  GLALP+ PYWWGLT+GGM+ TGAHGSSLWGKGSAVHDYV
Sbjct: 128 MTMTVESGMTLRQLIVEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYV 187

Query: 191 VEIQMVTPGGPQDGYAKLRRLLD--GDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITY 250
            EI+MV+PG   +G+AK+R L +       NAAKVSLGVLG+I+QVT +L+PMFKRS+TY
Sbjct: 188 TEIRMVSPGSVNEGFAKIRILSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLTY 247

Query: 251 LKRHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTP 310
             R+D D  D+ V+FG+ +EF D IW PSQ  VVYR+D RV+ NTSGNGL+DF+PFR   
Sbjct: 248 TMRNDSDFEDQAVTFGKKHEFADFIWLPSQGKVVYRRDDRVAVNTSGNGLFDFLPFRSQL 307

Query: 311 SVGLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRV 370
           S  + ++R SEE QE  RDA GKC  A +++S L   +YGLTNNGI F  YPV+G QNR+
Sbjct: 308 SAAIAIIRTSEETQERFRDANGKCVGATIISSTLFAPSYGLTNNGIIFTGYPVVGSQNRM 367

Query: 371 QSSGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAF 430
            SSGSCL  + +D  IT C WD  IKGEFFHQT +SV L  VKSFI D++ L ++E  + 
Sbjct: 368 MSSGSCL-DSLQDGLITACAWDSRIKGEFFHQTTLSVPLTQVKSFISDIKSLVKIEQKSL 427

Query: 431 CGLELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFK 490
           CGLEL+ GILMRYV +S AYLGK+ + +DFD TYYR++DP++PRL+ED +EE+EQ+A+FK
Sbjct: 428 CGLELHYGILMRYVTSSPAYLGKETEALDFDITYYRAKDPLTPRLYEDFIEEIEQIALFK 487

Query: 491 YGGTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDK 550
           Y   PHWGKNRN+AF   I KY +  AFL+VK+ YDP GLFS++WTDQ+LG+KG  +I K
Sbjct: 488 YNALPHWGKNRNLAFDGVIRKYNNAPAFLKVKDSYDPKGLFSSEWTDQILGIKGNASIVK 547

Query: 551 DGCALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYLGG 591
           DGCALEGLCICSKD+HCAP KGY CRPG+VYK+ARVC+ + G
Sbjct: 548 DGCALEGLCICSKDAHCAPAKGYLCRPGKVYKEARVCTRVDG 586

BLAST of Moc05g00180.1 vs. TAIR 10
Match: AT2G46740.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 750.0 bits (1935), Expect = 1.8e-216
Identity = 352/579 (60.79%), Postives = 440/579 (75.99%), Query Frame = 0

Query: 12  FRRFTAFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVYP 71
           +R     + LF L+     +PPEDP+KC S   N++C + NS G FPDRSTCRA +V YP
Sbjct: 13  WRTLLGLYCLFTLVHTVISTPPEDPVKCVS--GNTNCIVTNSLGAFPDRSTCRAANVAYP 72

Query: 72  TTQAEAISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAM 131
           TT+AE +S VAAAT+A RKM+V TR+ HSIPKL C DG DGL ISTK+LN T++ DA+AM
Sbjct: 73  TTEAELVSIVAAATKAGRKMRVTTRYSHSIPKLTCTDGNDGLFISTKFLNHTVQADAKAM 132

Query: 132 TITVEGGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVV 191
           T+TVE GVTLRQLI EAA  GLALP+ PYWWG+T+GGM+ TGAHGSSLWGKGSAVHDYV 
Sbjct: 133 TLTVESGVTLRQLIAEAAKVGLALPYAPYWWGVTVGGMMGTGAHGSSLWGKGSAVHDYVT 192

Query: 192 EIQMVTPGGPQDGYAKLRRLLD--GDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYL 251
           EI+MV+PG   DG+AK+R L +       NAAKVSLGVLG+I+QVT +L+PMFKRS+ Y+
Sbjct: 193 EIRMVSPGSVNDGFAKIRVLSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLKYV 252

Query: 252 KRHDRDLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPS 311
            R+D D  DE ++FG+ +EF D +W PSQ  VVYR D RV+ NT GNGLYDF PFR   S
Sbjct: 253 MRNDLDFNDEALTFGKKHEFADFVWLPSQGKVVYRMDDRVAVNTLGNGLYDFFPFRSQLS 312

Query: 312 VGLQLLRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQ 371
             L   R+SEE QE+ RDA GKC +A  ++S L  ++YGLTNNGITF  YPVIG QNR+ 
Sbjct: 313 AVLATTRSSEETQETLRDAHGKCVTATTISSTLFSTSYGLTNNGITFTGYPVIGSQNRMM 372

Query: 372 SSGSCLYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFC 431
           SSGSCL    +D   + C WD  +KG F+HQT  S+ L  VKSFI D++ L +++  + C
Sbjct: 373 SSGSCL-DGLEDKLTSACAWDSRVKGVFYHQTTFSIPLTQVKSFINDIKSLLKIDSKSLC 432

Query: 432 GLELYNGILMRYVKASSAYLGKQHDGVDFDFTYYRSRDPMSPRLFEDVLEEVEQMAVFKY 491
           GLELY GILMRYV +S AYLGK+ + +DFD TYYR+ DP++PRL+ED +EE+EQ+A+ KY
Sbjct: 433 GLELYYGILMRYVTSSPAYLGKETEAIDFDITYYRANDPLTPRLYEDFIEEIEQIALLKY 492

Query: 492 GGTPHWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKD 551
              PHWGKNRN+AF   I KY++  AFL+VK  YDP+GLFS++WTDQ+LG+KG  TI KD
Sbjct: 493 NALPHWGKNRNLAFDGVIKKYKNAPAFLKVKESYDPNGLFSSEWTDQILGIKGNPTIVKD 552

Query: 552 GCALEGLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYL 589
           GCALEGLCICS D+HCAP+KGY CRPG+VYK+ARVC+++
Sbjct: 553 GCALEGLCICSDDAHCAPSKGYLCRPGKVYKEARVCTHV 588

BLAST of Moc05g00180.1 vs. TAIR 10
Match: AT1G32300.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 724.9 bits (1870), Expect = 6.0e-209
Identity = 345/574 (60.10%), Postives = 430/574 (74.91%), Query Frame = 0

Query: 19  FFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQAEAI 78
           FF F     S  +PP+DP+KC S   N+ CT+ NSYG FPDRS C A  V YP T+AE +
Sbjct: 9   FFCFCTFASS--TPPDDPVKCES--GNNMCTVTNSYGAFPDRSICEAAKVEYPKTEAELV 68

Query: 79  SAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTITVEGG 138
           S VAAATRA +K++V TR+ HSIPKL C DG+DG+LISTK+LN  +  + EA T+TVE G
Sbjct: 69  SIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEAKTLTVESG 128

Query: 139 VTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQMVTP 198
           VTLRQLI EAA   LALPH PYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV EI+MV+P
Sbjct: 129 VTLRQLIGEAAELELALPHAPYWWGLTVGGLMGTGAHGSSLWGKGSAVHDYVSEIRMVSP 188

Query: 199 GGPQDGYAKLRRL---LDGDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHDRD 258
           G   DGY K+R L   +D D+   AAKVSLGVLG+I+QVT +L+PMFKRS+T++ ++D D
Sbjct: 189 GLASDGYVKVRVLSETIDPDE-FRAAKVSLGVLGVISQVTFQLQPMFKRSLTFVMQNDSD 248

Query: 259 LGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQLL 318
            GD+ V+FG  +EF D +W PSQ  VVYR D RV  NTSGNGL+DF PFRP  SV L ++
Sbjct: 249 FGDQAVTFGEKHEFADFLWLPSQGKVVYRMDDRVPVNTSGNGLFDFFPFRPQLSVALAII 308

Query: 319 RASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGSCL 378
           R+ EE +ES  DA  KC  A  +TS L   +YG+TNNG+ F  YPVIG QN + SSG+CL
Sbjct: 309 RSLEESEESSGDANDKCARAEQITSFLFSISYGVTNNGMEFTGYPVIGKQNHMMSSGTCL 368

Query: 379 YRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLELYN 438
             + +D  IT CPWDP IKG+FFHQTA S+ L  VK FI D++ L ++EP + C LE  N
Sbjct: 369 -DSHQDGLITSCPWDPRIKGQFFHQTAFSIPLTRVKGFINDIKALVKIEPKSLCALERSN 428

Query: 439 GILMRYVKASSAYLGKQHDGVDFDFTYYRSR-DPMSPRLFEDVLEEVEQMAVFKYGGTPH 498
           GIL+RYV +S A+LGK+   +DFD TYYRS+ DP++PRL+ED +EE+EQMA+FKY   PH
Sbjct: 429 GILIRYVTSSPAFLGKEEKALDFDLTYYRSKDDPLAPRLYEDFIEEIEQMAIFKYNALPH 488

Query: 499 WGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCALE 558
           WGKNRN+AF   I KY++   FL+VK  +DP GLFS +WT+Q+LGLKG VTI K+GCALE
Sbjct: 489 WGKNRNLAFDGVIRKYKNANTFLKVKERFDPLGLFSTEWTNQILGLKGNVTIVKEGCALE 548

Query: 559 GLCICSKDSHCAPNKGYFCRPGRVYKDARVCSYL 589
           GLC+CS D+HCAP KGY CRPG+VY  ARVC+++
Sbjct: 549 GLCVCSDDAHCAPKKGYLCRPGKVYTKARVCTHV 576

BLAST of Moc05g00180.1 vs. TAIR 10
Match: AT5G56490.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 721.1 bits (1860), Expect = 8.7e-208
Identity = 345/573 (60.21%), Postives = 428/573 (74.69%), Query Frame = 0

Query: 17  AFFFLFFLILGSECSPPEDPIKCRSPHDNSDCTIANSYGTFPDRSTCRARHVVYPTTQAE 76
           +  F FF    S  SPP+DP+ C     N+ CT+ NSYG FPDRS C+A  V YP T+AE
Sbjct: 6   SLIFCFFTFASS--SPPDDPVNCE--FGNTMCTVTNSYGAFPDRSICKAAKVEYPRTEAE 65

Query: 77  AISAVAAATRALRKMKVATRFYHSIPKLACPDGQDGLLISTKYLNRTLKVDAEAMTITVE 136
            +S VAAATRA +KM+V TR+ HS PKL C DG+DG LISTK+LN  +  + EA T+TVE
Sbjct: 66  LVSIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEAKTLTVE 125

Query: 137 GGVTLRQLIEEAATAGLALPHTPYWWGLTIGGMVSTGAHGSSLWGKGSAVHDYVVEIQMV 196
            GVTLRQLIEEAA   LALP+ PYWWGLT+GGM+ TGAHGSSLWGKGSAVHDYV EI++V
Sbjct: 126 SGVTLRQLIEEAAKFDLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRLV 185

Query: 197 TPGGPQDGYAKLRRLLD--GDQHLNAAKVSLGVLGIITQVTLKLEPMFKRSITYLKRHDR 256
           +PG   DGY K++ L +    +   AAKVSLGVLG+I+QVT +L+PMFKRS+ Y+ R+D 
Sbjct: 186 SPGLASDGYVKVQVLSETIDPEEFRAAKVSLGVLGVISQVTFELQPMFKRSLNYVMRNDS 245

Query: 257 DLGDEVVSFGRNYEFGDLIWYPSQRMVVYRQDSRVSSNTSGNGLYDFIPFRPTPSVGLQL 316
           D GD+ VSFG  +EF D +W PSQ  VVYR D RV  NTSG+GL++F PFR   S+ L +
Sbjct: 246 DFGDQAVSFGERHEFADFLWLPSQGKVVYRMDGRVPLNTSGDGLFEFFPFRSQLSLVLAI 305

Query: 317 LRASEEDQESRRDAGGKCFSARLVTSLLSFSAYGLTNNGITFLKYPVIGYQNRVQSSGSC 376
            R+ EE +ES  DA  KC  A+LV+S +   +YG+TNNG+ F  YPVIG QN + SSGSC
Sbjct: 306 DRSLEESEESLEDANMKCVRAKLVSSSMFLMSYGVTNNGLIFTGYPVIGMQNHMMSSGSC 365

Query: 377 LYRNAKDMRITGCPWDPLIKGEFFHQTAVSVELRVVKSFIEDVQKLAELEPMAFCGLELY 436
           L  + +D  IT CPWDP IKG+FFHQT  SV L  VKSFI D++ L ++EP + C LE  
Sbjct: 366 L-DSRQDGLITACPWDPRIKGQFFHQTTFSVSLTNVKSFINDIKALVKIEPKSLCVLEGS 425

Query: 437 NGILMRYVKASSAYLGKQHDGVDFDFTYYRSR-DPMSPRLFEDVLEEVEQMAVFKYGGTP 496
           NGIL+RYV +S A+LGK+   +DFD TYYRS+ DP++PRL+ED +EE+EQMA+FKY   P
Sbjct: 426 NGILIRYVTSSLAFLGKEEKALDFDLTYYRSKNDPLAPRLYEDYIEEIEQMAIFKYNALP 485

Query: 497 HWGKNRNVAFQEAIAKYRDKEAFLEVKNEYDPHGLFSNQWTDQVLGLKGAVTIDKDGCAL 556
           HWGKNRN+AF  AI KY++  AFL+VK ++D  GLFS +WTDQ+LGLKG VTI K GCAL
Sbjct: 486 HWGKNRNLAFDGAIRKYKNANAFLKVKEKFDSLGLFSTEWTDQILGLKGNVTIVKQGCAL 545

Query: 557 EGLCICSKDSHCAPNKGYFCRPGRVYKDARVCS 587
           EGLCICS+DSHCAP KGY CRPG+VY++ARVC+
Sbjct: 546 EGLCICSEDSHCAPTKGYLCRPGKVYREARVCT 573