Moc04g36440.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g36440.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Calcium-binding EF-hand family protein
Location	chr4: 27351723 .. 27352277 (+)
Sequence length	555
RNA-Seq Expression	Moc04g36440.1
Synteny	Moc04g36440.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAAGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCTGGTTTCAATTGA

mRNA sequence

ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAAGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCTGGTTTCAATTGA

Coding sequence (CDS)

ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAAGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCTGGTTTCAATTGA

Protein sequence

MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGFN

Homology

[Close]

BLAST of Moc04g36440.1 vs. NCBI nr
Match: XP_022133625.1 (probable calcium-binding protein CML45 [Momordica charantia])

HSP 1 Score: 373.2 bits (957), Expect = 1.3e-99
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP
Sbjct: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60

Query: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120
           KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA
Sbjct: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120

Query: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180
           FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME
Sbjct: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180

Query: 181 SGFN 185
           SGFN
Sbjct: 181 SGFN 184

[Close]

BLAST of Moc04g36440.1 vs. NCBI nr
Match: XP_038889669.1 (probable calcium-binding protein CML46 [Benincasa hispida])

HSP 1 Score: 203.4 bits (516), Expect = 1.7e-48
Identity = 110/184 (59.78%), Postives = 134/184 (72.83%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSP-I 60
           MD  +YN+SM +KT   +NPS RFLI+ID L FQKL SWL    + FA+T+      P  
Sbjct: 1   MDFGVYNVSMDDKT-TTQNPSFRFLIVIDFLRFQKLASWL----YAFATTKQKPSSFPAT 60

Query: 61  PKAVVDGGES--VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEV 120
            K    GGE   ++ EEMK VMG+LGI CGE   ES+PLFGS EE+A++F+ER     EV
Sbjct: 61  SKTEEHGGEERLMTTEEMKWVMGNLGIDCGE---ESVPLFGSTEEVAEMFDERAASTVEV 120

Query: 121 KRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVK 180
           K+AFDVFD NGDGFID  ELQRV+CVLG KEGE ++NC+RMI+KFD N DG+IDFEEF+K
Sbjct: 121 KKAFDVFDVNGDGFIDSEELQRVMCVLGFKEGERIQNCERMIRKFDHNGDGRIDFEEFLK 176

Query: 181 LMES 182
           LMES
Sbjct: 181 LMES 176

[Close]

BLAST of Moc04g36440.1 vs. NCBI nr
Match: XP_022993675.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 199.5 bits (506), Expect = 2.5e-47
Identity = 104/170 (61.18%), Postives = 121/170 (71.18%), Query Frame = 0


Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAV-----VDGGESVS 76
           P NPS RFLIMIDC  FQKL SWL    + FAS +      P  +        +GGE VS
Sbjct: 7   PANPSFRFLIMIDCFRFQKLGSWL----YAFASGKRKQWSLPASETAKSEQRKEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VM +LG+  GEE +ESLP F S+EE+ K+F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMES 182
           IDV ELQRVLCVLG KEGE ++NC+RMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]

BLAST of Moc04g36440.1 vs. NCBI nr
Match: XP_031741770.1 (probable calcium-binding protein CML47 [Cucumis sativus])

HSP 1 Score: 198.4 bits (503), Expect = 5.5e-47
Identity = 107/184 (58.15%), Postives = 135/184 (73.37%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPI- 60
           M+L LYN+SM +K+  P+NPS RFL++ID L FQ L SWL ++     ST+      P  
Sbjct: 132 MNLGLYNVSMDDKS-TPQNPSFRFLLIIDFLRFQNLASWLCSS----TSTKLKQSTPPAS 191

Query: 61  PKAVVDGGES--VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEV 120
            K+V    E   VS EEMK VMG+LGI C E    S+PLFGS EE+A++F++    +AEV
Sbjct: 192 SKSVESRAEERLVSTEEMKWVMGNLGIDCSE--GSSVPLFGSAEEVAEMFDQTAASVAEV 251

Query: 121 KRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVK 180
           K+AF VFD NGDGFIDV ELQRV+CVLG KEGEG+ENC++MI+KFD N+DG+IDFEEFVK
Sbjct: 252 KQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVK 308

Query: 181 LMES 182
           LME+
Sbjct: 312 LMEA 308

[Close]

BLAST of Moc04g36440.1 vs. NCBI nr
Match: XP_008459700.2 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 197.2 bits (500), Expect = 1.2e-46
Identity = 108/186 (58.06%), Postives = 133/186 (71.51%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           M+  LYN+SM +K+  P+NPS RFLI+ID L FQ L SWL +    FAST+      P  
Sbjct: 1   MNFGLYNVSMDDKS-TPQNPSFRFLIIIDFLRFQNLASWLCS----FASTKLKQSTLPAS 60

Query: 61  KAVVD--GGES---VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIA 120
              V+   GE    VS EEMK VMG+LG+ C E    S+PLFGS EE+A++F++     A
Sbjct: 61  SKSVESSAGERERLVSTEEMKWVMGNLGMDCSE--GSSVPLFGSAEEVAEMFDQTAASAA 120

Query: 121 EVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEF 180
           EVK+AFDVFD N DGFIDV ELQRV+CVLG KEGEG+ENC++MI KFD N+DG+IDFEEF
Sbjct: 121 EVKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEF 179

Query: 181 VKLMES 182
           VKLME+
Sbjct: 181 VKLMEA 179

[Close]

BLAST of Moc04g36440.1 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 6.6e-27
Identity = 61/119 (51.26%), Postives = 85/119 (71.43%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D  + + RE++  VM  LG+   +E +E L    S +E++ LFEE+ P + EVK+AFDVF
Sbjct: 86  DDDDGLCREDVGMVMKSLGLSTDQE-NEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVF 145

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGF 184
           D N DGFID ++LQRVL +LGLK+G  +ENC+RMI+ FD ++DG+IDF  FVK ME+ F
Sbjct: 146 DENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNF 203

[Close]

BLAST of Moc04g36440.1 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-25
Identity = 61/119 (51.26%), Postives = 79/119 (66.39%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D    +SREE + VM  LG+      D+ L    S +E++ LFEE+   + EVK+AFDVF
Sbjct: 78  DDDIDISREEAEMVMRSLGLF---YNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVF 137

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGF 184
           D N DGFID +ELQRVL +LG K+G  ++NC  MI+  D N+DGKIDF EFVK ME+ F
Sbjct: 138 DENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]

BLAST of Moc04g36440.1 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.7e-14
Identity = 51/144 (35.42%), Postives = 73/144 (50.69%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFG----------SVEEIAKLF------- 124
           +G   ++REE++  +G LGI      DE   +             VEE  +L+       
Sbjct: 103 NGDGRITREELEDSLGKLGIPV--PADELAAVIARIDANGDGCVDVEEFGELYRSIMAGG 162

Query: 125 ---------EERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRM 183
                    EE      +++ AF VFDANGDG+I V EL  VL  LGLK+G   E C+RM
Sbjct: 163 DDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQGRTAEECRRM 222

[Close]

BLAST of Moc04g36440.1 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.2e-14
Identity = 61/154 (39.61%), Postives = 87/154 (56.49%), Query Frame = 0


Query: 39  WLSATYHGFASTRNSNRCSPIP---KAVVDGGESV--SREEMKSVMGD---LGIVCGEEG 98
           + S  Y  F S RN+ + + +P   K VV     +  S EE+K+++ D   L     EEG
Sbjct: 42  FFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEG 101

Query: 99  DESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGE 158
           +E L     +E+   + +E       VK AF +FD N DGFID  EL+ VL +LG  E  
Sbjct: 102 EEYL-----LEKNEMMGKE------IVKEAFRLFDENQDGFIDENELKHVLSLLGYDECT 161

Query: 159 GVENCKRMIQKFDENEDGKIDFEEFVKLMESGFN 185
            +E C++M++ +DEN DGKIDF EFVKL+E  F+
Sbjct: 162 KME-CRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of Moc04g36440.1 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.8e-14
Identity = 47/129 (36.43%), Postives = 76/129 (58.91%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEG----DESLPLFG----SVEEIAKLFE---ERGPC 124
           +G   ++++E+   + +LGI   ++      E + L G     +EE   L++   E    
Sbjct: 16  NGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDE 75

Query: 125 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFE 183
             +++ AF+VFD N DGFI V EL+ VL  LGLK+G  +E+CKRMI K D + DG ++F+
Sbjct: 76  EEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFK 135

[Close]

BLAST of Moc04g36440.1 vs. ExPASy TrEMBL
Match: A0A6J1BVS4 (probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC111006164 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 6.1e-100
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP
Sbjct: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60

Query: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120
           KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA
Sbjct: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120

Query: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180
           FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME
Sbjct: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180

Query: 181 SGFN 185
           SGFN
Sbjct: 181 SGFN 184

[Close]

BLAST of Moc04g36440.1 vs. ExPASy TrEMBL
Match: A0A6J1JZ70 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111489598 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.2e-47
Identity = 104/170 (61.18%), Postives = 121/170 (71.18%), Query Frame = 0


Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAV-----VDGGESVS 76
           P NPS RFLIMIDC  FQKL SWL    + FAS +      P  +        +GGE VS
Sbjct: 7   PANPSFRFLIMIDCFRFQKLGSWL----YAFASGKRKQWSLPASETAKSEQRKEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VM +LG+  GEE +ESLP F S+EE+ K+F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMES 182
           IDV ELQRVLCVLG KEGE ++NC+RMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]

BLAST of Moc04g36440.1 vs. ExPASy TrEMBL
Match: A0A0A0KS94 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.7e-47
Identity = 107/184 (58.15%), Postives = 135/184 (73.37%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPI- 60
           M+L LYN+SM +K+  P+NPS RFL++ID L FQ L SWL ++     ST+      P  
Sbjct: 2   MNLGLYNVSMDDKS-TPQNPSFRFLLIIDFLRFQNLASWLCSS----TSTKLKQSTPPAS 61

Query: 61  PKAVVDGGES--VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEV 120
            K+V    E   VS EEMK VMG+LGI C E    S+PLFGS EE+A++F++    +AEV
Sbjct: 62  SKSVESRAEERLVSTEEMKWVMGNLGIDCSE--GSSVPLFGSAEEVAEMFDQTAASVAEV 121

Query: 121 KRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVK 180
           K+AF VFD NGDGFIDV ELQRV+CVLG KEGEG+ENC++MI+KFD N+DG+IDFEEFVK
Sbjct: 122 KQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVK 178

Query: 181 LMES 182
           LME+
Sbjct: 182 LMEA 178

[Close]

BLAST of Moc04g36440.1 vs. ExPASy TrEMBL
Match: A0A1S3CAB3 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 6.0e-47
Identity = 108/186 (58.06%), Postives = 133/186 (71.51%), Query Frame = 0


Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           M+  LYN+SM +K+  P+NPS RFLI+ID L FQ L SWL +    FAST+      P  
Sbjct: 1   MNFGLYNVSMDDKS-TPQNPSFRFLIIIDFLRFQNLASWLCS----FASTKLKQSTLPAS 60

Query: 61  KAVVD--GGES---VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIA 120
              V+   GE    VS EEMK VMG+LG+ C E    S+PLFGS EE+A++F++     A
Sbjct: 61  SKSVESSAGERERLVSTEEMKWVMGNLGMDCSE--GSSVPLFGSAEEVAEMFDQTAASAA 120

Query: 121 EVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEF 180
           EVK+AFDVFD N DGFIDV ELQRV+CVLG KEGEG+ENC++MI KFD N+DG+IDFEEF
Sbjct: 121 EVKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEF 179

Query: 181 VKLMES 182
           VKLME+
Sbjct: 181 VKLMEA 179

[Close]

BLAST of Moc04g36440.1 vs. ExPASy TrEMBL
Match: A0A6J1FKT4 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111445127 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 4.3e-45
Identity = 100/170 (58.82%), Postives = 119/170 (70.00%), Query Frame = 0


Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVV-----DGGESVS 76
           P  PS RFLIMIDC  FQKL SWL    + FA+ +      P  +        +GGE VS
Sbjct: 7   PAIPSFRFLIMIDCFRFQKLGSWL----YAFATGKRKQWSLPASETAKSEQRDEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VMG+LG+   EE +ESLP F S+EE+ ++F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMES 182
           ID  ELQRVLCVLG KEGE ++NC+RMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]

BLAST of Moc04g36440.1 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 122.1 bits (305), Expect = 4.7e-28
Identity = 61/119 (51.26%), Postives = 85/119 (71.43%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D  + + RE++  VM  LG+   +E +E L    S +E++ LFEE+ P + EVK+AFDVF
Sbjct: 86  DDDDGLCREDVGMVMKSLGLSTDQE-NEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVF 145

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGF 184
           D N DGFID ++LQRVL +LGLK+G  +ENC+RMI+ FD ++DG+IDF  FVK ME+ F
Sbjct: 146 DENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNF 203

[Close]

BLAST of Moc04g36440.1 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-26
Identity = 61/119 (51.26%), Postives = 79/119 (66.39%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D    +SREE + VM  LG+      D+ L    S +E++ LFEE+   + EVK+AFDVF
Sbjct: 78  DDDIDISREEAEMVMRSLGLF---YNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVF 137

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGF 184
           D N DGFID +ELQRVL +LG K+G  ++NC  MI+  D N+DGKIDF EFVK ME+ F
Sbjct: 138 DENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]

BLAST of Moc04g36440.1 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.6e-15
Identity = 61/154 (39.61%), Postives = 87/154 (56.49%), Query Frame = 0


Query: 39  WLSATYHGFASTRNSNRCSPIP---KAVVDGGESV--SREEMKSVMGD---LGIVCGEEG 98
           + S  Y  F S RN+ + + +P   K VV     +  S EE+K+++ D   L     EEG
Sbjct: 42  FFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEG 101

Query: 99  DESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGE 158
           +E L     +E+   + +E       VK AF +FD N DGFID  EL+ VL +LG  E  
Sbjct: 102 EEYL-----LEKNEMMGKE------IVKEAFRLFDENQDGFIDENELKHVLSLLGYDECT 161

Query: 159 GVENCKRMIQKFDENEDGKIDFEEFVKLMESGFN 185
            +E C++M++ +DEN DGKIDF EFVKL+E  F+
Sbjct: 162 KME-CRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of Moc04g36440.1 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-15
Identity = 47/129 (36.43%), Postives = 76/129 (58.91%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEEG----DESLPLFG----SVEEIAKLFE---ERGPC 124
           +G   ++++E+   + +LGI   ++      E + L G     +EE   L++   E    
Sbjct: 16  NGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDE 75

Query: 125 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFE 183
             +++ AF+VFD N DGFI V EL+ VL  LGLK+G  +E+CKRMI K D + DG ++F+
Sbjct: 76  EEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFK 135

[Close]

BLAST of Moc04g36440.1 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 79.3 bits (194), Expect = 3.5e-15
Identity = 45/132 (34.09%), Postives = 76/132 (57.58%), Query Frame = 0


Query: 65  DGGESVSREEMKSVMGDLGIVCGEE------------GDESLPL--FGSVEEIAKLFEER 124
           DG   ++ +E+     +LGI+  E+            GD  + +  FG + +   + +E 
Sbjct: 16  DGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTIMVEDED 75

Query: 125 GPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKI 183
                ++K AF+VFD NGDGFI V EL+ VL  LGLK+G+ +E C++MI + D + DG++
Sbjct: 76  EVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRV 135

The following BLAST results are available for this feature:

BLAST of Moc04g36440.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133625.1	1.3e-99	100.00	probable calcium-binding protein CML45 [Momordica charantia]	[more]
XP_038889669.1	1.7e-48	59.78	probable calcium-binding protein CML46 [Benincasa hispida]	[more]
XP_022993675.1	2.5e-47	61.18	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]
XP_031741770.1	5.5e-47	58.15	probable calcium-binding protein CML47 [Cucumis sativus]	[more]
XP_008459700.2	1.2e-46	58.06	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]

BLAST of Moc04g36440.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z27	6.6e-27	51.26	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9MBG5	2.1e-25	51.26	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q0JC44	1.7e-14	35.42	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SN89	2.2e-14	39.61	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
Q9SRR7	3.8e-14	36.43	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]

BLAST of Moc04g36440.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVS4	6.1e-100	100.00	probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1JZ70	1.2e-47	61.18	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]
A0A0A0KS94	2.7e-47	58.15	Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1	[more]
A0A1S3CAB3	6.0e-47	58.06	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 P...	[more]
A0A6J1FKT4	4.3e-45	58.82	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of Moc04g36440.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39670.1	4.7e-28	51.26	Calcium-binding EF-hand family protein	[more]
AT3G29000.1	1.5e-26	51.26	Calcium-binding EF-hand family protein	[more]
AT3G47480.1	1.6e-15	39.61	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	2.7e-15	36.43	ARF-GAP domain 11	[more]
AT4G03290.1	3.5e-15	34.09	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 116..144 e-value: 0.0027 score: 26.9 coord: 154..182 e-value: 1.7E-4 score: 31.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 116..180 e-value: 1.7E-12 score: 47.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..184 score: 13.202322
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 112..147 score: 14.262346
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 116..179 e-value: 2.21093E-17 score: 70.6545
None	No IPR available	GENE3D	1.10.238.10		coord: 34..184 e-value: 5.0E-21 score: 77.2
None	No IPR available	PANTHER	PTHR10891:SF805	CALCIUM-BINDING PROTEIN CML45-RELATED	coord: 58..183
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 58..183
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 125..137
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 64..180

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc04g36440	Moc04g36440	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc04g36440.1.exon1	Moc04g36440.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc04g36440.1	cds.Moc04g36440.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc04g36440.1	Moc04g36440.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding